Consulta: subjectFacets:"Osteoblast"
Registros recuperados: 11
Data/hora: 09/06/2024 13:21:43

Potential effects of a low molecular weight Fucoidan extracted from brown algae on bone biomaterial osteoconductive properties
Provedor de dados: 5
Autores: Igondjo Tchen Changotade, S; Korb, G; Bassil, J; Barroukh, B; Willig, C; Colliec-jouault, Sylvia; Durand, Patrick; Godeau, G; Senni, K.
In this work we firstly tested the influence of low molecular weight fucoidan extracted from pheophicae cell wall on bidimensional cultured normal human osteoblasts behaviours. Secondly impregnation procedure with LMW fucoidan of bone biomaterial (Lubboc®) we explored in this bone extracellular matrix context its capabilities to support human osteoblastic behaviour in 3D culture. In bidimensionnal cultures we evidenced that: LMW fucoidan promotes human osteoblast proliferation, collagen type I expression and favours precocious alkaline phosphatase activity. Furthermore with LMW fucoidan von Kossa's staining was positive at 30 days and only positive at 45 days in absence of LMW fucoidan. In our three dimensional culture models with the biomaterial...
Tipo: Text
Palavras-chave: Heparan mimetics; Biomaterial; Bone; Osteoblast; Fucoidan.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2007/publication-2695.pdf

Cytocompatibility, cytotoxicity and genotoxicity analyses of dental implants
Provedor de dados: 46
Autores: Reigosa,M.; Labarta,V.; Molinari,G.; Bernales,D.
Different materials are used for prostheses in dental medicine, though titanium is the most frequent. In the present report, several commercial dental implants and their potential capacity to integrate to bone tissues have been analysed by means of cytotoxicity, genotoxicity and cytocompatibility techniques. Cultures from osteosarcoma-derived UMR 106 cell line were used for bioassays since they showed several osteoblast properties. Samples of dental implants provided by B&amp;M national company (ASTM titanium 8348 GR2, acid-etched surface) were compared with some others commercialized by foreign firms in Argentina (Nobel: anodic-oxidized surface, and 3i: sandblasted surface). Using the neutral red uptake test, lysosome and alkaline phosphatase enzymatic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Cytocompatibility; Single cell electrophoresis; Osteoblast.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332008000100007

Integrin-&amp;#946;1, not integrin-&amp;#946;5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain
Provedor de dados: 48
Autores: Zeng,Qiangcheng; Guo,Yong; Liu,Yongming; Li,Ruixin; Zhang,Xinchang; Liu,Lu; Wang,Yang; Zhang,Xizheng; Zou,Xianqiong.
BACKGROUND: Mechanical strain plays a great role in growth and differentiation of osteoblast. A previous study indicated that integrin-&amp;#946; (&amp;#946;1, &amp;#946;5) mediated osteoblast proliferation promoted by mechanical tensile strain. However, the involvement of integrin-&amp;#946; in osteoblastic differentiation and extracellular matrix (ECM) formation induced by mechanical tensile strain, remains unclear. RESULTS: After transfection with integrin-&amp;#946;1 siRNA or integrin-&amp;#946;5 siRNA, mouse MC3T3-E1 preosteoblasts were cultured in cell culture dishes and stimulated with mechanical tensile strain of 2500 microstrain (µ&amp;#949;) at 0.5 Hz applied once a day for 1 h over 3 or 5 consecutive days. The cyclic tensile strain promoted...
Tipo: Journal article
Palavras-chave: Tensile strain; Osteoblast; Extracellular matrix; Integrin; SiRNA.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100025

In vitro culture and characterization of alveolar bone osteoblasts isolated from type 2 diabetics
Provedor de dados: 56
Autores: Sun,Dao-Cai; Li,De-Hua; Ji,Hui-Cang; Rao,Guo-Zhou; Liang,Li-Hua; Ma,Ai-Jie; Xie,Chao; Zou,Gui-Ke; Song,Ying-Liang.
In order to understand the mechanisms of poor osseointegration following dental implants in type 2 diabetics, it is important to study the biological properties of alveolar bone osteoblasts isolated from these patients. We collected alveolar bone chips under aseptic conditions and cultured them in vitro using the tissue explants adherent method. The biological properties of these cells were characterized using the following methods: alkaline phosphatase (ALP) chemical staining for cell viability, Alizarin red staining for osteogenic characteristics, MTT test for cell proliferation, enzyme dynamics for ALP contents, radio-immunoassay for bone gla protein (BGP) concentration, and ELISA for the concentration of type I collagen (COL-I) in the supernatant....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alveolar bone; Cell culture; Implant; Osteoblast; Type 2 diabetics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600005

Effect of magnesium ion on human osteoblast activity
Provedor de dados: 56
Autores: He,L.Y.; Zhang,X.M.; Liu,B.; Tian,Y.; Ma,W.H.
Magnesium, a promising biodegradable metal, has been reported in several studies to increase bone formation. Although there is some information regarding the concentrations of magnesium ions that affect bone remodeling at a cellular level, little is known about the effect of magnesium ions on cell gap junctions. Therefore, this study aimed to systematically investigate the effects of different concentrations of magnesium on bone cells, and further evaluate its effect on gap junctions of osteoblasts. Cultures of normal human osteoblasts were treated with magnesium ions at concentrations of 1, 2 and 3 mM, for 24, 48 and 72 h. The effects of magnesium ions on viability and function of normal human osteoblasts and on gap junction intercellular communication...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Magnesium; Osteoblast; Gap junction intercellular communication.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700604

Tissue transglutaminase (TG2) activity regulates osteoblast differentiation and mineralization in the SAOS-2 cell line
Provedor de dados: 56
Autores: Yin,Xiaoxue; Chen,Zhongqiang; Liu,Zhongjun; Song,Chunli.
Tissue transglutaminase (type II, TG2) has long been postulated to directly promote skeletal matrix calcification and play an important role in ossification. However, limited information is available on the expression, function and modulating mechanism of TG2 during osteoblast differentiation and mineralization. To address these issues, we cultured the well-established human osteosarcoma cell line SAOS-2 with osteo-inductive conditioned medium and set up three time points (culture days 4, 7, and 14) to represent different stages of SAOS-2 differentiation. Osteoblast markers, mineralization, as well as TG2 expression and activity, were then assayed in each stage. Furthermore, we inhibited TG activity with cystamine and then checked SAOS-2 differentiation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transglutaminase; Osteoblast; Differentiation; Mineralization; Bone morphogenetic protein-2 (BMP-2).
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800002

In vitro studies of multiwalled carbon nanotube/ultrahigh molecular weight polyethylene nanocomposites with osteoblast-like MG63 cells
Provedor de dados: 56
Autores: Reis,J.; Kanagaraj,S.; Fonseca,A.; Mathew,M.T.; Capela-Silva,F.; Potes,J.; Pereira,A.; Oliveira,M.S.A.; Simões,J.A.
Carbon nanotubes are highly versatile materials; new applications using them are continuously being developed. Special attention is being dedicated to the possible use of multiwalled carbon nanotubes in biomaterials contacting with bone. However, carbon nanotubes are also controversial in regards to effects exerted on living organisms. Carbon nanotubes can be used to improve the tribological properties of polymer/composite materials. Ultrahigh molecular weight polyethylene (UHMWPE) is a polymer widely used in orthopedic applications that imply wear and particle generation. We describe here the response of human osteoblast-like MG63 cells after 6 days of culture in contact with artificially generated particles from both UHMWPE polymer and multiwalled carbon...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanocomposites; Carbon nanotubes; Wear particle; Osteoblast; Orthopedic; MG63 cells.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500009

The effects of 6-gingerol on proliferation, differentiation, and maturation of osteoblast-like MG-63 cells
Provedor de dados: 56
Autores: Fan,J.Z.; Yang,X.; Bi,Z.G.
We investigated whether 6-gingerol affects the maturation and proliferation of osteoblast-like MG63 cells in vitro. Osteoblast-like MG63 cells were treated with 6-gingerol under control conditions, and experimental inflammation was induced by tumor necrosis factor-α (TNF-α). Expression of different osteogenic markers and cytokines was analyzed by real-time PCR, Western blotting, and enzyme-linked immunosorbent assay. In addition, alkaline phosphatase (ALP) enzyme activity and biomineralization as markers for differentiation were measured. Treatment with 6-gingerol resulted in insignificant effects on the proliferation rate. 6-Gingerol induced the differentiation of osteoblast-like cells with increased transcription levels of osteogenic markers, upregulated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 6-Gingerol; Interleukin-6; Nuclear factor-κB; Osteogenesis; Osteoblast.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700637

Serotonin regulates osteoblast proliferation and function in vitro
Provedor de dados: 56
Autores: Dai,S.Q.; Yu,L.P.; Shi,X.; Wu,H.; Shao,P.; Yin,G.Y.; Wei,Y.Z.
The monoamine serotonin (5-hydroxytryptamine, 5-HT), a well-known neurotransmitter, also has important functions outside the central nervous system. The objective of this study was to investigate the role of 5-HT in the proliferation, differentiation, and function of osteoblasts in vitro. We treated rat primary calvarial osteoblasts with various concentrations of 5-HT (1 nM to 10 µM) and assessed the rate of osteoblast proliferation, expression levels of osteoblast-specific proteins and genes, and the ability to form mineralized nodules. Next, we detected which 5-HT receptor subtypes were expressed in rat osteoblasts at different stages of osteoblast differentiation. We found that 5-HT could inhibit osteoblast proliferation, differentiation, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serotonin; Osteoblast; Proliferation; Differentiation; 5-HT receptors; 5-HT.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900759

Single-walled carbon nanotubes functionalized with sodium hyaluronate enhance bone mineralization
Provedor de dados: 56
Autores: Sá,M.A.; Ribeiro,H.J.; Valverde,T.M.; Sousa,B.R.; Martins-Júnior,P.A.; Mendes,R.M.; Ladeira,L.O.; Resende,R.R.; Kitten,G.T.; Ferreira,A.J.
The aim of this study was to evaluate the effects of sodium hyaluronate (HY), single-walled carbon nanotubes (SWCNTs) and HY-functionalized SWCNTs (HY-SWCNTs) on the behavior of primary osteoblasts, as well as to investigate the deposition of inorganic crystals on titanium surfaces coated with these biocomposites. Primary osteoblasts were obtained from the calvarial bones of male newborn Wistar rats (5 rats for each cell extraction). We assessed cell viability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay and by double-staining with propidium iodide and Hoechst. We also assessed the formation of mineralized bone nodules by von Kossa staining, the mRNA expression of bone repair proteins, and the deposition of inorganic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Osteoblast; Nanotechnology; Carbon nanotubes; Sodium hyaluronate; Tissue engineering; Osseointegration.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200602

Neuropeptide Y, substance P, and human bone morphogenetic protein 2 stimulate human osteoblast osteogenic activity by enhancing gap junction intercellular communication
Provedor de dados: 56
Autores: Ma,W.H.; Liu,Y.J.; Wang,W.; Zhang,Y.Z.
Bone homeostasis seems to be controlled by delicate and subtle “cross talk” between the nervous system and “osteo-neuromediators” that control bone remodeling. The purpose of this study was to evaluate the effect of interactions between neuropeptides and human bone morphogenetic protein 2 (hBMP2) on human osteoblasts. We also investigated the effects of neuropeptides and hBMP2 on gap junction intercellular communication (GJIC). Osteoblasts were treated with neuropeptide Y (NPY), substance P (SP), or hBMP2 at three concentrations. At various intervals after treatment, cell viability was measured by the MTT assay. In addition, cellular alkaline phosphatase (ALP) activity and osteocalcin were determined by colorimetric assay and radioimmunoassay,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuropeptide; Bone morphogenetic protein; Osteoblast; Gap junction intercellular communication.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400299