Consulta: subjectFacets:"Oyster vasa like gene"
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Data/hora: 03/06/2024 11:51:44

Origine et développement des cellules germinales chez l'huître creuse Crassostrea gigas : Intérêt pour le contrôle de la reproduction en écloserie.
Provedor de dados: 5
Autores: Fabioux, Caroline.
A comprehensive knowledge of Crassostrea gigas oyster reproduction processes is essential to optimize controlled reproduction in hatchery. This study aimed to determine the origin and the mechanisms of annual renewal of germ cells, still unknown in oysters, and the influence of temperature and photoperiod parameters on the regulation of the reproductive cycle. We characterized an orthologue of the vasa gene, the Oyvlg (Oyster vasa-like gene), the first germ cell specific marker isolated in molluscs. This marker was used to study C. gigas germline development. Oyster germ cells appeared specified during early development from mesentoblast 4d, as primordial germ cells (PGCs) determined by maternal cytoplasmic determinants, including Oyvlg mRNA. In adult...
Tipo: Text
Palavras-chave: Controlled reproduction; Regulation; Oyster vasa like gene; Gametogenesis; Germ cells; Crassostrea; Reproduction contrôlée; Régulation; Oyster vasa like gene; Gamétogenèse; Cellules germinales; Crassostrea.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/these-1380.pdf

Oyster vasa-like gene as a marker of the germline cell development in Crassostrea gigas
Provedor de dados: 5
Autores: Fabioux, Caroline; Huvet, Arnaud; Lelong, C; Robert, Rene; Pouvreau, Stephane; Daniel, Jean-yves; Mingant, Christian; Le Pennec, M.
The oyster vasa-like gene was previously demonstrated to be specifically expressed in germline cells of adult oysters Crassostrea gigas. In the present study, this gene was used as a molecular marker to establish the developmental pattern of germline cells during oyster ontogenesis. using whole-mount in situ hybridization and real-time PCR. The Oyvlg transcripts appeared to be localized to the vegetal pole of unfertilized oocytes and maternally transmitted to embryos. At early development, these maternal transcripts were observed to segregate into a single blastomere, from the CD macromere of 2-cell stage to the 4d mesentoblast of blastula. From late blastula stage, the mesentoblast divided into two cell clumps that migrated to both sides of the larvae...
Tipo: Text
Palavras-chave: Primordial germ cells; Oyster vasa like gene; Germline; Development; Crassostrea gigas.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-648.pdf