Consulta: subjectFacets:"PCR en temps réel"
Registros recuperados: 3
Data/hora: 06/06/2024 13:00:46

Quantification des bactéries histaminogènes et maîtrise de la formation d'histamine dans les produits marins par biopréservation
Provedor de dados: 5
Autores: Podeur, Gaetan.
Histamine-producing bacteria were isolated from naturally contaminated seafood, some of responsible for histamine-poisoning. At the same time, lactic acid bacteria were isolated on the same products and clustered according to their inhibitory activity against 4 histamine-producing species. Most of the strains were identified as Lactobacillus fuchuensis or Lactobacillus sakei. Twelve lactic acid bacteria were selected for challenge-test on canned, cooked or smoked tuna. Challenge-test combined with sensory analysis performed on cooked vacuum-packed tuna against Morganella psychrotolerans and Morganella morganii, demonstrated an important inhibitory effect on growth and production of histamine with L. sakei CNCM I-4707. Bacterial count was reduced by 3 to 5...
Tipo: Text
Palavras-chave: Biopréservation; Thon; Bactérie lactique; Intoxication histaminique; Morganella morganii; Morganella psychrotolerans; Lactobacillus sakei; Analyse sensorielle; PCR en temps réel; Étape d'enrichissement; RT-qPCR; Biopreservation; Tuna; Lactic acid bacteria; Scombroid poisoning; Morganella morganii; Morganella psychrotolerans; Lactobacillus sakei; Sensory analysis; Real-time PCR; Enrichment step; RT-qPCR.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00241/35203/33709.pdf

Etude des effets de polluants sur les paramètres hémocytaires de l'huître creuse, Crassostrea gigas - Interactions entre environnement, mécanismes de défense et maladies infectieuses
Provedor de dados: 5
Autores: Gagnaire, Beatrice.
Shellfish industry is mostly realized in estuary zones, which are subjected to pollutions due to anthropic activities. The harmful effects of pollutants on animals inhabiting these estuarine zones are poorly known. Among these animals, Pacific oyster, Crassostrea gigas, may represent a model because they are sedentary and they filter water intensively. Among all physiological functions possibly disturbed by pollutants, defence mechanisms are poorly studied in bivalves. Moreover, animals presenting impaired defence mechanisms may be more sensitive to infectious diseases. In this context, effects of pollutants on hemocyte functions and on sensitiveness to diseases were tested in C. gigas. After adjusting the protocols for the monitoring of hemocyte...
Tipo: Text
Palavras-chave: Real time PCR; Flow cytometry; Pesticides; Pollutants; Phagocytosis; Hemocytes; Crassostrea gigas; Pacific oyster; Pathology; Immunotoxicology; PCR en temps réel; Cytométrie de flux; Pesticides; Polluants; Phagocytose; Hémocytes; Crassostrea gigas; Huître creuse; Pathologie; Immunotoxicologie.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/these-730.pdf

Etude des interactions hôte/virus chez l’huître creuse Crassostrea gigas et son virus Ostreid herpesvirus 1
Provedor de dados: 5
Autores: Segarra, Amelie.
Ostreid herpesvirus type 1 (OsHV-1) can be considered one of the major infectious agents in Pacific oysters, Crassostrea gigas, in France and all around the world. Differences in terms of mortality among infected animals were reported suggesting a genetic basis of the susceptibility to the OsHV-1 infection. In this context, the main objective of this thesis was to understand the interactions between Pacific oysters and OsHV-1, in particular, the molecular basis of the viral cycle. Results showed that the virus is able to replicate in the host regardless of its stage of development or its susceptibility. However, multiplication kinetics is faster in the most susceptible individuals compared to less susceptible ones. After an active replication phase, it...
Tipo: Text
Palavras-chave: Bivalves; Huître creuse; Crassostrea gigas; Mortalités; OsHV-1; Gènes viruax; Interaction hôte-virus; Transcriptome; Sensibilité; PCR en temps réel; Bivalve; Pacific oyster; Crassostrea ggias; Mortality; OsHV-1; Viral gene; Interaction host-virus; Transcriptome; Susceptibility; Real-time PCR.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00252/36297/34826.pdf