Consulta: subjectFacets:"Paralytic shellfish poisoning"
Registros recuperados: 11
Data/hora: 11/06/2024 07:59:04

Paralytic shellfish poison accumulation yields and feeding time activity in the Pacific oyster (Crassostrea gigas) and king scallop (Pecten maximus)
Provedor de dados: 5
Autores: Bougrier, Serge; Lassus, Patrick; Bardouil, Michele; Masselin, Pierre; Truquet, Philippe.
Pacific oysters and king scallops placed individually in a recirculating flume were fed for 2 weeks with a constant concentration (120 cell ml(-1)) of a toxic strain of Alexandrium minutum. Fluorescence at the outlet of each experimental unit was measured continuously, and biodeposits were recovered twice daily to evaluate feeding time activity (FTA) and rates of organic filtration (OFR), ingestion (OIR) and organic absorption (OAR). Ion-pairing high performance liquid chromatography (IP-HPLC) was performed concurrently to quantify paralytic phycotoxins both (i) individually in shellfish at the end of the contamination period and (ii) in A. minutum cultures to estimate cellular toxin concentration. These data allowed comparison of the actual tissue toxin...
Tipo: Text
Palavras-chave: Alexandrium minutum; Crassostrea gigas; Feeding activity; Paralytic shellfish poisoning.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-576.pdf

Pre-purification by membrane filtration of paralytic shellfish toxins from Alexandrium minutum dinoflagellate
Provedor de dados: 5
Autores: Balti, Rafik; Brodu, Nicolas; Zhang, Jiaxuan; Amzil, Zouher; Drouin, Delphine; Sechet, Veronique; Masse, Anthony.
The Paralytic shellfish neurotoxins (PST) are of increasing interest for biomedical applications. The chemical synthesis is often complex and expensive that’s why the purification by membrane filtration of PST from Alexandrium minutum dinoflagellate was investigated. Disrupted micro-alga cells by ultrasonic treatment were diafiltred to let pass toxins through an ultrafiltration membrane. Then, the mean permeate was concentrated and diafiltrated by nanofiltration. Mean permeate fluxes equal to 187, 135 and 135 L.h–1.m-2 were obtained during the first diafiltration, the concentration step and the final diafiltration respectively. Up to 57 % (mol/mol) and 78 % (mol/mol) of organic matters and salts were removed respectively. Divalent ions were sparsely...
Tipo: Text
Palavras-chave: Paralytic shellfish poisoning; Nanofiltration; Membrane; Alexandrium minutum; Purification.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00451/56286/57865.pdf

Viability, growth and toxicity of Alexandrium catenella and Alexandrium minutum (Dinophyceae) following ingestion and gut passage in the oyster Crassostrea gigas
Provedor de dados: 5
Autores: Laabir, Mohamed; Amzil, Zouher; Lassus, Patrick; Masseret, Estelle; Tapilatu, Yosmina; De Vargas, Romain; Grzebyk, Daniel.
Adult oysters Crassostrea gigas were experimentally fed with Alexandrium catenella and Alexandrium minutum which are responsible for recurrent toxic blooms in French coastal waters. C. gigas produced faeces and pseudofaeces containing intact and viable temporary pellicular cysts of these two Paralytic toxin producing species. When incubated in favourable conditions, these pellicular cysts were able to germinate at high rates (between 74 and 94%) and the resulting vegetative cells divided with growth rates close to the non-ingested cells (control). The toxin profile of the vegetative cells originated from the germinated temporary cysts was analyzed by liquid chromatography/ fluorescence detection. Total toxin content of newly germinated cells was lower than...
Tipo: Text
Palavras-chave: Paralytic shellfish poisoning; Oysters; Cysts; Alexandrium minutum; Alexandrium catenella.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2622.pdf

Effect of the PSP-causing dinoflagellate, Alexandrium sp. on the initial feeding response of Crassostrea gigas
Provedor de dados: 5
Autores: Wildish, David; Lassus, Patrick; Martin, Jennifer; Saulnier, Alinne; Bardouil, Michele.
The effects of toxic and non-toxic strains of Alexandrium sp. on the initial feeding responses of the Pacific oyster Crassostrea gigas were determined by continuous monitoring of the clearance rate of individual oysters. This was determined fluorometrically by loss of fluorescence of live microalgae introduced as food. Feeding oysters with toxic or non-toxic A. tamarense and toxic A. fundyense resulted in stop/start clearance behaviour. In contrast, control oysters fed a reference microalga, Isochrysis sp., known to support their growth, did not exhibit this behavior. Pacific oysters acclimated to Isochrysis sp., fed mixtures of Alexandrium/Isochrysis, showed further evidence of stop/start clearance. Exposure for periods up to 48 hours with non-toxic A....
Tipo: Text
Palavras-chave: Crassostrea gigas; Physiologie alimentaire; Microalgues toxiques; Dinoflagellés; Paralytic shellfish poisoning; Crassostrea gigas; Feeding physiology; Toxic microalgae; Dinoflagellate; Paralytic shellfish poisoning.
Ano: 1998
URL: https://archimer.ifremer.fr/doc/1998/publication-877.pdf

Influence of Crassostrea gigas (Thunberg) sexual maturation stage and ploidy on uptake of paralytic phycotoxins
Provedor de dados: 5
Autores: Gueguen, Marielle; Baron, Regis; Bardouil, Michele; Haberkorn, Hansy; Soudant, Philippe; Truquet, Philippe; Lassus, Patrick.
The purpose of this study was to assess paralytic phycotoxin uptake in diploid and triploid oysters at two stages of their sexual cycle corresponding to their status in early summer (June) and winter (November). Samples of diploid and triploid oysters were exposed to a toxic culture of Alexandrium minutum for 4 days in each season. No significant differences in filtration or clearance rates were observed during either November or June experiments. When diploid oysters were at resting stage (November), toxin uptake showed no significant difference between the ploidy classes. In contrast, when the diploid oysters were at the peak of their sexual maturation (June), the triploid oysters were seen to accumulate almost double the amount of paralytic toxins as...
Tipo: Text
Palavras-chave: Paralytic shellfish poisoning; Crassostrea gigas; Diploid; Triploid; Contamination.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00087/19796/17487.pdf

Sources of paralytic shellfish toxin accumulation variability in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Pousse, Emilien; Flye-sainte-marie, Jonathan; Alunno-bruscia, Marianne; Hegaret, Helene; Jean, Fred.
This study was designed to assess the contribution of feeding behavior to inter-individual variability of paralytic shellfish toxin (PST) accumulation in the Pacific oyster Crassostrea gigas. For this purpose 42 oysters were exposed for 2 days to non-toxic algae and then for 2 other days to the PST producer Alexandrium minutum. Individual clearance rate (CR) of oysters was continuously monitored over the 4 days using an ecophysiological measurement system. Comparison of CR values when exposed to toxic and non toxic algae allowed to estimate a clearance rate inhibition index (CRII). Toxin concentration of oysters was quantified at the end of the experiment. These data allowed to estimate the toxin accumulation efficiency (TAE) as the ratio of toxin...
Tipo: Text
Palavras-chave: Alexandrium minutum; Paralytic shellfish poisoning; Accumulation; Clearance rate; Feeding behavior; Pacific oyster.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00416/52789/53688.pdf

Saxitoxin Modulates Immunological Parameters and Gene Transcription in Mytilus chilensis Hemocytes
Provedor de dados: 5
Autores: Astuya, Allisson; Carrera, Crisleri; Ulloa, Viviana; Aballay, Ambbar; Nunez-acuna, Gustavo; Hegaret, Helene; Gallardo-escarate, Cristian.
Saxitoxin (STX) is a neurotoxin produced by dinoflagellates in diverse species, such as Alexandrium spp., and it causes paralytic shellfish poisoning (PSP) in humans after the ingestion of contaminated shellfish. Recent studies have suggested that the immune functions of bivalves could be affected by harmful algae and/or by their toxins. Herein, hemocytes are the main effector cells of the immune cellular response. In this study, we evaluated the response of hemocytes from the mussel Mytilus chilensis to STX exposure in a primary culture. Cell cultures were characterized according to size and complexity, while reactive oxygen species (ROS) production was evaluated using a dichlorofluorescein diacetate (DCFH-DA) assay. Finally, phagocytic activity was...
Tipo: Text
Palavras-chave: Saxitoxin; Hemocytes; Immune response; Paralytic shellfish poisoning; Reactive oxygen species (ROS).
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00278/38954/73180.pdf

Modelling the accumulation of PSP toxins in Thau Lagoon oysters (Crassostrea gigas) from trials using mixed cultures of Alexandrium catenella and Thalassiosira weissflogii
Provedor de dados: 5
Autores: Lassus, Patrick; Amzil, Zouher; Baron, Regis; Sechet, Veronique; Barille, Laurent; Abadie, Eric; Bardouil, Michele; Sibat, Manoella; Truquet, Philippe; Berard, Jean-baptiste; Gueguen, Marielle.
In October and November 2003 a bloom of the toxic dinoflagellate Alexandrium catenella was observed in the North-east zone of Thau lagoon (French Mediterranean coast). Sea water samples were collected every hours to evaluate time-related variations of phytoplankton concentrations and to compare the relative ratio of A. catenella versus other phytoplankton species during the outbreak. From these observations, trials using recirculated sea water systems were performed to: i) evaluate the physiological effects on oyster of increasing proportions of A. catenella within a mixed microalgal diet where the non-toxic diatom Thalassiosira weissflogii was present at concentration: 1500 cells ml(-1), ii) compare the effect of two temperatures (12 degrees C and 18...
Tipo: Text
Palavras-chave: Mediterranean Sea; Alexandrium catenella; Toxin content; Model; Crassostrea gigas; Paralytic shellfish poisoning.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2624.pdf

Detoxification of Pacific oyster Crassostrea gigas fed on diets of Skeletonema costatum with and without silt, following PSP contamination by Alexandrium minutum
Provedor de dados: 5
Autores: Gueguen, Marielle; Bardouil, Michele; Baron, Regis; Lassus, Patrick; Truquet, Philippe; Massardier, Julie; Amzil, Zouher.
Contamination of shellfish by paralytic shellfish poisoning ( PSP) toxins poses an economic threat to shellfish farmers. As contaminated shellfish cannot be harvested for long periods of time, it would be very useful to develop processes to optimise and shorten their detoxification. In this study, Pacific oysters Crassostrea gigas were first experimentally contaminated over a period of 13 days with a continuous flow of toxic Alexandrium minutum cultures at concentrations ranging from 150 to 200 cell ml(-1) ( toxin content after 13 days of contamination 438 mu g STX equiv. 100 g(-1) wet weight). Then, two different detoxification treatments were tested and showed detoxification rates greater than those observed in coastal environments. The first treatment...
Tipo: Text
Palavras-chave: Alexandrium minutum; Crassostrea gigas; Detoxification; Inorganic matter; Organic matter; PSP; Paralytic shellfish poisoning.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3896.pdf

Detection of shellfish toxins from scallops in Guangzhou seafood market
Provedor de dados: 80
Autores: Huazhang,L; Wen-xue,L; Quan-xin,Z; Guang-yu,Y; Jun-tao,L; Li,M; Wei,Z.
To evaluate scallop safety in the Guangzhou seafood market, contents of shellfish toxins in adductor muscle, mantle skirts, gills and visceral mass of scallops were examined using enzyme-linked immunosorbent assay (ELISA) and mouse unit assay. The results showed that: paralytic shellfish poisoning contents were up to 37.44 μg/100 g by ELISA and 319.99 MU/100 g by mouse unit assay, which did not exceed the limits of national standards (80 μg/100g and 400 MU/100 g); the contents of diarrhetic shellfish poisoning were 142.04 μg/100g and 0.2 MU/100 g, which exceeded the national standard limits (60 μg/100g); neurotoxic shellfish poisoning was undetectable; the contents of amnesic shellfish poisoning reached 220.12 μg/100g (no limit value could be referred to)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paralytic shellfish poisoning; Diarrheic shellfish poisoning; Neurotoxic shellfish poisoning; Amnesic shellfish poisoning; Enzyme-linked immunosorbent assay; Mouse unit assay.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100010

Human intoxication with paralytic shellfish toxins: Clinical parameters and toxin analysis in plasma and urine
Provedor de dados: 48
Autores: GARCÍA,CARLOS; LAGOS,MARCELO; TRUAN,DOMINIQUE; LATTES,KARINNA; VÉJAR,OMAR; CHAMORRO,BEATRIZ; IGLESIAS,VERÓNICA; ANDRINOLO,DARÍO; LAGOS,NÉSTOR.
This study reports the data recorded from four patients intoxicated with shellfish during the summer 2002, after consuming ribbed mussels (Aulacomya ater) with paralytic shellfish toxin contents of 8,066 ± 61.37 mg/100 gr of tissue. Data associated with clinical variables and paralytic shellfish toxins analysis in plasma and urine of the intoxicated patients are shown. For this purpose, the evolution of respiratory frequency, arterial blood pressure and heart rate of the poisoned patients were followed and recorded. The clinical treatment to reach a clinically stable condition and return to normal physiological parameters was a combination of hydration with saline solution supplemented with Dobutamine (vasoactive drug), Furosemide (diuretic) and Ranitidine...
Tipo: Journal article
Palavras-chave: Paralytic shellfish poisoning; PSP human intoxication; Chilean fjords.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200009