Consulta: subjectFacets:"Periodontitis"
Registros recuperados: 13
Data/hora: 05/06/2024 12:44:47

Stem Cells and Androgen Receptor in Human Periapical Periodontitis
Provedor de dados: 77
Autores: Rodríguez,H; Espinoza-Navarro,O; Peñafiel,C; Escobar,E; Silva,I; Quiroz,P; Arriaza,C; Castro,M. E; Sánchez,C; Arias,M; Michea,A.
Activation of macrophages in periapical granulomas occurs through the presence of cytokines, endotoxin and other genetic and epigenetic factors, allowing the initiation of inflammation and bone resorption. The present study aims to analyze the presence of CD133 protein (marker of stem cells) and the AR (androgen receptor) protein in biopsies of human odontogenic periapical granuloma. Biopsies from 14 adult male patients with diagnosis of periapical granuloma included in paraffin blocks were processed histologically to obtain 5-µm thick sections. Protein presence was detected and analyzed by immunohistochemistry of CD133 and AR. The quantification considered the number of positive cells in 0.17 mm2 random areas under the microscope using a 1000X objective....
Tipo: Journal article
Palavras-chave: Stem cell; Androgen receptor; Granuloma; Periodontitis.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300048

Porphyromonas gingivalis LIBRE DE POLISACÁRIDOS UTILIZANDO CROMATOGRAFÍA DE ALTA RESOLUCIÓN SEPHACRYL S-200
Provedor de dados: 37
Autores: GUALTERO,DIEGO; CASTELLANOS,JAIME E; PÉREZ,GERARDO; LAFAURIE,GLORIA I.
El objetivo de este trabajo fue mejorar un método estándar para la purificación de lipopolisacárido (LPS) de Porphyromonas gingivalis libre de polisacáridos usando una estrategia de extracción, digestión enzimática y cromatografía de alta resolución. La bacteria P. gingivalis se cultivó en condiciones de anaerobiosis y se hizo extracción de las membranas con el método de fenol-agua. Luego de una digestión enzimática (DNAsa, RNAsa y proteasa) se separó el extracto por filtración por gel con Sephacryl S-200. La muestra purificada se caracterizó por electroforesis en gel de acrilamida con tinción de plata y por el método Purpald se detecto el ácido 2-ceto-3-desoxioctu-losónico (KDO). Se obtuvo una preparación libre de ácidos nucleicos, proteínas y...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Periodontitis; Lipopolisacáridos; Endotoxinas; Cromatografía.
Ano: 2008
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2008000300012

Low-dose doxycycline prevents inflammatory bone resorption in rats
Provedor de dados: 56
Autores: Bezerra,M.M.; Brito,G.A.C.; Ribeiro,R.A.; Rocha,F.A.C.
Matrix metalloproteinases (MMP) are considered to be key initiators of collagen degradation, thus contributing to bone resorption in inflammatory diseases. We determined whether subantimicrobial doses of doxycycline (DX) (&lt;=10 mg kg-1 day-1), a known MMP inhibitor, could inhibit bone resorption in an experimental periodontitis model. Thirty male Wistar rats (180-200 g) were subjected to placement of a nylon thread ligature around the maxillary molars and sacrificed after 7 days. Alveolar bone loss (ABL) was measured macroscopically in one hemiarcade and the contralateral hemiarcade was processed for histopathologic analysis. Groups of six animals each were treated with DX (2.5, 5 or 10 mg kg-1 day-1, sc, 7 days) and compared to nontreated (NT) rats. NT...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tetracyclines; Doxycycline; Metalloproteinases; Periodontitis; Osteoporosis; Bone resorption; Inflammation.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500015

Antimicrobial peptides and nitric oxide production by neutrophils from periodontitis subjects
Provedor de dados: 56
Autores: Mariano,F.S.; Campanelli,A.P.; Nociti Jr.,F.H.; Mattos-Graner,R.O.; Gonçalves,R.B.
Neutrophils play an important role in periodontitis by producing nitric oxide (NO) and antimicrobial peptides, molecules with microbicidal activity via oxygen-dependent and -independent mechanisms, respectively. It is unknown whether variation in the production of antimicrobial peptides such as LL-37, human neutrophil peptides (HNP) 1-3, and NO by neutrophils influences the pathogenesis of periodontal diseases. We compared the production of these peptides and NO by lipopolysaccharide (LPS)-stimulated neutrophils isolated from healthy subjects and from patients with periodontitis. Peripheral blood neutrophils were cultured with or without Aggregatibacter actinomycetemcomitans-LPS (Aa-LPS), Porphyromonas gingivalis-LPS (Pg-LPS) and Escherichia coli-LPS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial peptides; Nitric oxide; Neutrophils; Periodontitis; Innate immunity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100004

Effects of fluoride on proliferation and mineralization in periodontal ligament cells in vitro
Provedor de dados: 56
Autores: Li,K.Q.; Jia,S.S.; Ma,M.; Shen,H.Z.; Xu,L.; Liu,G.P.; Huang,S.Y.; Zhang,D.S.
Fluoride, which is often added to toothpaste or mouthwash in order to protect teeth from decay, may be a novel therapeutic approach for acceleration of periodontal regeneration. Therefore, we investigated the effects of fluoride on proliferation and mineralization in human periodontal ligament cells in vitro. The periodontal ligament cells were stimulated with various concentrations of NaF added into osteogenic inductive medium. Immunohistochemistry of cell identification, cell proliferation, alkaline phosphatase (ALP) activity assay, Alizarin red S staining and quantitative real-time-polymerase chain reaction (RT-PCR) were performed. Moderate concentrations of NaF (50-500 μmol/L) had pro-proliferation effects, while 500 μmol/L had the best effects. ALP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluoride; Periodontitis; Periodontal ligament cells; Mineralization.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800601

Comparison of etoricoxib and indomethacin for the treatment of experimental periodontitis in rats
Provedor de dados: 56
Autores: Azoubel,M.C.F.; Menezes,A.M.A.; Bezerra,D.; Oriá,R.B.; Ribeiro,R.A.; Brito,G.A.C.
We investigated the effect of etoricoxib, a selective cyclooxygenase-2 inhibitor, and indomethacin, a non-selective cyclooxygenase inhibitor, on experimental periodontitis, and compared their gastrointestinal side effects. A ligature was placed around the second upper left molars of female Wistar rats (160 to 200 g). Animals (6 per group) were treated daily with oral doses of 3 or 9 mg/kg etoricoxib, 5 mg/kg indomethacin, or 0.2 mL saline, starting 5 days after the induction of periodontitis, when bone resorption was detected, until the sacrifice on the 11th day. The weight and survival rate were monitored. Alveolar bone loss (ABL) was measured as the sum of distances between the cusp tips and the alveolar bone. The gastric mucosa was examined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alveolar bone loss; Inflammation; Periodontitis; Cyclooxygenase inhibitors; Etoricoxib; Indomethacin.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100015

Distribution of biotypes and leukotoxic activity of Aggregatibacter actinomycetemcomitans isolated from Brazilian patients with chronic periodontitis
Provedor de dados: 58
Autores: Gaetti-Jardim Jr.,Elerson; Wahasugui,Thais Cristiane; Tomazinho,Paulo Henrique; Marques,Márcia Martins; Nakano,Viviane; Avila-Campos,Mario Julio.
Aggregatibacter actinomycetemcomitans is an important etiologic agent of the periodontitis and is associated with extra-oral infections. In this study, the detection of the ltxA gene as well as the ltx promoter region from leukotoxic A. actinomycetemcomitans isolated from 50 Brazilian patients with periodontitis and 50 healthy subjects was performed. The leukotoxic activity on HL-60 cells was also evaluated. Leukotoxic activity was determined using a trypan blue exclusion method. The 530 bp deletion in the promoter region was evaluated by PCR using a PRO primer pair. A. actinomycetemcomitans was detected by culture and directly from crude subgingival biofilm by PCR using specific primers. By culture, A. actinomycetemcomitans was detected in nine (18%) of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotype; Leukotoxin; Periodontitis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400011

In vitro antagonistic growth effects of Lactobacillus fermentum and Lactobacillus salivarius and their fermentative broth on periodontal pathogens
Provedor de dados: 58
Autores: Chen,Ling-Ju; Tsai,Hsiu-Ting; Chen,Wei-Jen; Hsieh,Chu-Yang; Wang,Pi-Chieh; Chen,Chung-Shih; Wang,Lina; Yang,Chi-Chiang.
As lactobacilli possess an antagonistic growth property, these bacteria may be beneficial as bioprotective agents for infection control. However, whether the antagonistic growth effects are attributed to the lactobacilli themselves or their fermentative broth remains unclear. The antagonistic growth effects of Lactobacillus salivarius and Lactobacillus fermentum as well as their fermentative broth were thus tested using both disc agar diffusion test and broth dilution method, and their effects on periodontal pathogens, including Streptococcus mutans, Streptococcus sanguis, and Porphyromonas gingivalisin vitro at different concentrations and for different time periods were also compared. Both Lactobacillus salivarius and Lactobacillus fermentum and their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus fermentum; Lactobacillus salivarius; Streptococcus mutans; Streptococcus sanguis; Porphyromonas gingivalis; Periodontitis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400019

Expression of HMGB1 and HMGN2 in gingival tissues, GCF and PICF of periodontitis patients and peri-implantitis
Provedor de dados: 58
Autores: Xie,Ping; Deng,Lu-Xia; Gong,Ping; Ding,Yi; Tang,Xiao-Hai.
High mobility group chromosomal protein B1 (HMGB1) and N2 (HMGN2), two members of High mobility group (HMG) family, play important role in inflammation. The purposes of this study were to investigate the expression of HMGB1 and HMGN2 in periodontistis. The expression of HMGB1 and HMGN2 mRNA in gingival tissues and gingival crevicular fluid (GCF) in chronic periodontitis (CP), generalized aggressive periodontitis (G-AgP) patients and healthy subjects was detected by real-time PCR. The protein level of HMGB1 and HMGN2 in peri-implant crevicular fluid (PICF), peri-implant crevicular fluid of peri-implantitis (PI-PICF) and normal patients was determined by Western blotting. Furthermore, IL-1β, IL-6, IL-8, TNF-α and HMGB1 levels in GCF, PI-PICF and healthy-PICF...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HMGB1; HMGN2; Periodontitis; Peri-implantitis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300047

Detection of putative periodontal pathogens in subgingival specimens of dogs
Provedor de dados: 58
Autores: Nishiyama,Sheila Alexandra Belini; Senhorinho,Gerusa Neyla Andrade; Gioso,Marco Antônio; Avila-Campos,Mario Julio.
In this study, the presence of putative periodontal organisms, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, Fusobacterium nucleatum,Dialister pneumosintes,Actinobacillus actinomycetemcomitans,Campylobacter rectus,Eikenella corrodens and Treponema denticola were examined from subgingival samples of 40 dogs of different breeds with (25) and without (15) periodontitis, by using the PCR method. The PCR products of each species showed specific amplicons. Of the 25 dogs with periodontitis, P. gingivalis was detected in 16 (64%) samples, C. rectus in 9 (36%), A. actinomycetemcomitans in 6 (24%), P. intermedia in 5 (20%), T. forsythensis in 5 (20%), F. nucleatum in 4 (16%) and E. corrodens in 3 (12%). T. denticola and D. pneumosintes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Periodontal microorganisms; Dogs; Periodontitis; Oral anaerobic bacteria.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100006

Prevalence of Pseudomonas aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with chronic periodontal infection
Provedor de dados: 58
Autores: Souto,Renata; Silva-Boghossian,Carina M.; Colombo,Ana Paula Vieira.
P. aeruginosa and Acinetobacter spp. are important pathogens associated with late nosocomial pneumonia in hospitalized and institutionalized individuals. The oral cavity may be a major source of these respiratory pathogens, particularly in the presence of poor oral hygiene and periodontal infection. This study investigated the prevalence of P. aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with periodontal disease or health. Samples were obtained from 55 periodontally healthy (PH) and 169 chronic periodontitis (CP) patients. DNA was obtained from the samples and detection of P. aeruginosa and Acinetobacter spp. was carried out by multiplex and nested PCR. P. aeruginosa and Acinetobacter spp. were detected in 40% and 45% of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pseudomonas aeruginosa; Acinetobacter spp; Subgingival biofilm; Saliva; Periodontitis; PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200017

Staphylococcus spp. in the oral cavity and periodontal pockets of chronic periodontitis patients
Provedor de dados: 58
Autores: Loberto,Jussara Cia S.; Martins,Clélia Ap. de Paiva; Santos,Silvana S. Ferreira dos; Cortelli,José Roberto; Jorge,Antonio Olavo Cardoso.
Staphylococcus spp are not usually isolated from the oral cavity, and when this occurs, they are considered to belong to the transitory microbiota. Individuals with periodontal disease represent possible reservoirs of these opportunistic bacteria in the oral cavity. The use of antibiotics for treatment of periodontal disease or other infections may predispose to the increase of the number of Staphylococcus spp. in the oral cavity. These microorganisms easily become resistant to antibiotics, and may result in superinfection. The purpose of this study was to evaluate the presence of Staphylococcus spp. in the oral cavity and in periodontal pockets of patients with chronic periodontitis, identify the isolates and verify the relationship between the presence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus; Opportunistic bacteria; Periodontitis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100010

Occurrence of periodontal pathogens among patients with chronic periodontitis
Provedor de dados: 58
Autores: Farias,B.C.; Souza,P.R.E.; Ferreira,B.; Melo,R.S.A.; Machado,F.B.; Gusmão,E.S.; Cimões,R.
The aim of the present study was to evaluate the presence of the periodontal pathogens that form the red complex (Tannerella forsythia, Porphyromonas gingivalis and Treponema denticola) and Aggregatibacter actinomycetemcomitans in patients with chronic periodontitis. The sample consisted of 29 patients with a clinical and radiographic diagnosis of chronic periodontitis based on the criteria of the American Academy of Periodontology (3). Samples for microbiological analysis were collected from the four sites of greatest probing depth in each patient, totaling 116 samples. These samples were processed using conventional polymerase chain reaction, which achieved the following positive results: 46.6% for P. gingivalis, 41.4% for T. forsythia, 33.6% for T....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Periodontitis; Pathogens; Tannerella forsythia; Porphyromonas gingivalis; Treponema denticola; Aggregatibacter actinomycetemcomitans.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300009