Consulta: subjectFacets:"Petunia × atkinsiana D. Don"
Registros recuperados: 1
Data/hora: 12/06/2024 11:14:24

BSA and molecular markers screening for salt stress tolerant mutant of Petunia obtained in in vitro culture
Provedor de dados: 65
Autores: Krupa-Małkiewicz,Marcelina; Bienias,Anna.
ABSTRACT: In this study, we performed BSA to identify genetic markers linked to salt tolerance. We tested the genetic diversity among four bulked DNA samples of EMS induced mutant clones and one bulked DNA sample of non-mutated clone of Petunia for salt tolerance in in vitro callus cultures using RAPD and ISSR markers. Out of the 36 RAPD and 16 ISSR primers identified, 25 and 13 were effectively used to amplify genomic DNA of all the five bulked samples, respectively. In total, 114 RAPD amplifications products were obtained, of which 28% were polymorphic and 2% were genotype-specific bands. Out of the 64 ISSR amplification products obtained, 51% were polymorphic and 1% was genotype-specific bands. Results of this study indicated the existence of two...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bulk segregant analysis; Chemical mutagenesis; ISSR; RAPD; Petunia × atkinsiana D. Don; Salt stress; Tissue culture.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001200400