Consulta: subjectFacets:"Phagocytose"
Registros recuperados: 7
Data/hora: 10/06/2024 07:45:46

Modulation des paramètres hémocytaires par la nutrition de l'huitre creuse Crassostrea gigas. Implication dans les mortalités estivales
Provedor de dados: 5
Autores: Delaporte, Maryse.
In the context of Pacific oyster C. gigas summer mortality, the present study demonstrated that the reproductive effort reduced not only the energetic parameters (carbohydrates and CEA) but also the immune parameters (concentration, phagocytosis and adhesive capacity of hemocytes). Increasing food supply from 4% to 12% (of oyster dry weight) did not counteract this phenomena as most of the additional energy was dedicated to the gametogenesis processes. However, after the gametogenesis, the 12% diet clearly favored energy storage restoration and hemocyte production. Hemocyte parameters were also modulated by the food quality. Indeed, hemocyte concentration tended to increase 20:5n-3 and/or 20:4n-6 were supplied by micro-algae or artificial diets. When these...
Tipo: Text
Palavras-chave: Summer mortalities; Flow cytometer; Immunity; Oxidative burst; Phagocytosis; Biochemical composition hemocyte parameters; Essential fatty acids; Nutrition; Crassostrea gigas; Mortalités estivales; Cytomètre de flux; Immunité; Poussée respiratoire; Phagocytose; Réponses hémocytaires; Composition biochimique; Acides gras essentiels; Nutrition; Crassostrea gigas.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/these-357.pdf

Etude des amibes marines et de leurs interactions avec les vibrios pathogènes d’huître
Provedor de dados: 5
Autores: Robino, Etienne.
Free living amoebae inhabit aquatic environments and use phagocytosis of bacteria for their nutrition. According to the hypothesis of coincidental evolution of virulence, the cellular and molecular mechanisms of phagocytosis being preserved from amoebae to the immune cells of animals, the predation exerted by amoebae could favor the emergence of pathogenic bacteria resistant to phagocytosis. Since 2008, Crassostrea gigas oysters have suffered from over-mortality in France. This poly-microbial disease involves the Herpes OsHV-1 μvar virus which causes an immunosuppression of oysters that are then colonized by various opportunistic pathogenic bacteria including vibrios inducing the death of the animal. V. tasmaniensis LGP32 is a facultative intracellular...
Tipo: Text
Palavras-chave: Amibes libres; Vibrios pathogènes; Phagocytose; Évolution fortuite; Diversité; Free-Living amoebae; Pathogenic vibrios; Phagocytosis; Coincidental evolution; Diversity.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00692/80419/83555.pdf

Bonamia ostreae, parasite de l'huître plate, Ostrea edulis: sa position taxonomique parmi les parasites du groupe "microcell", analyses des interactions hôte/parasite chez plusieurs populations d'huîtres plates
Provedor de dados: 5
Autores: Besnard-cochennec, Nathalie.
Bonamia ostreae is the causative agent of hemocyte disease. It has been detected first during mortalities occured in Franc in 1979. Bonamiosis spread rapidly among oysters farming in France and Europ. Another species, Bonamia sp. has been detected in New Zealand and Australia. These paasites were both included in the "microcell" group. This group contains 2 others parasites, Mikrocytos mackini affecting Crassostrea gigas in British Columbia (Canada) and M. roughleyi affecing Saccostrea glomerata in Australia. Structural and molecular caracterisation of all these parasites were realised. Results show tha B. ostreae, B. sp., B. exitiosus. Molecular tools are available for genus detection, Bonamia spp. and Mikrocytos and species identification (B. ostreae, B....
Tipo: Text
Palavras-chave: Bonamia ostreae; Ostrea edulis; Taxonomie; Diagnotic; PCR; In situ hybridation; Hémocytes; Lectines; Phagocytose; Résistance; Bonamia ostreae; Ostrea edulis; Taxonomy; Diagnotic; PCR; In situ hybridization; Hemocytes; Lectins; Phagocytosis; Resistance.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00211/32223/30671.pdf

Caractérisation morphologique et fonctionnelle des hémocytes d'Ostrea edulis et de Crassostrea gigas, mollusques bivalves. Etude in vitro de leurs interactions avec le protozoaire Bonamia ostreae (Ascetospora)
Provedor de dados: 5
Autores: Jean Chagot, Dominique.
La morphologie et l'équipement enzymatique des hémocytes de deux espèces d'huîtres, Ostea edulis et Crassostrea gigas ont été étudiés. L'analyse des fonctions des hémocytes dans les mécanismes immunitaires a permis de mettre en évidence leur rôle de macrophage, mais n'a pas fait apparaître de différence interspécifique dans les phénomènes des cytocidie oxygène dépendants, susceptible d'expliquer la permissivité d'Ostrea edulis pour Bonamia ostreae et la résistance de Crassostrea gigas. L'infection in vitro de primocultures d'hémocytes des deux espèces démontre un mécanisme d'entrée par phagocytose, doublé chez Ostrea edulis d'une possible pénétration active. L'analyse des récepteurs impliqués dans la phagocytose (récepteurs pour des opsonines plasmatiques,...
Tipo: Text
Palavras-chave: Ostrea edulis; Crassostrea gigas; Bonamia ostreae; Hémocyte; Morphologie; Macrophage; Caractérisation enzymatiques; Chimioluminescence; Interaction in vitro; Phagocytose; Récepteur.
Ano: 1989
URL: http://archimer.ifremer.fr/doc/00210/32102/30559.pdf

Etude des effets de polluants sur les paramètres hémocytaires de l'huître creuse, Crassostrea gigas - Interactions entre environnement, mécanismes de défense et maladies infectieuses
Provedor de dados: 5
Autores: Gagnaire, Beatrice.
Shellfish industry is mostly realized in estuary zones, which are subjected to pollutions due to anthropic activities. The harmful effects of pollutants on animals inhabiting these estuarine zones are poorly known. Among these animals, Pacific oyster, Crassostrea gigas, may represent a model because they are sedentary and they filter water intensively. Among all physiological functions possibly disturbed by pollutants, defence mechanisms are poorly studied in bivalves. Moreover, animals presenting impaired defence mechanisms may be more sensitive to infectious diseases. In this context, effects of pollutants on hemocyte functions and on sensitiveness to diseases were tested in C. gigas. After adjusting the protocols for the monitoring of hemocyte...
Tipo: Text
Palavras-chave: Real time PCR; Flow cytometry; Pesticides; Pollutants; Phagocytosis; Hemocytes; Crassostrea gigas; Pacific oyster; Pathology; Immunotoxicology; PCR en temps réel; Cytométrie de flux; Pesticides; Polluants; Phagocytose; Hémocytes; Crassostrea gigas; Huître creuse; Pathologie; Immunotoxicologie.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/these-730.pdf

Contribution à l'étude de vibrions pathogènes d'huîtres creuses Crassostrea gigas - Interactions hémocytes-vibrions et développement de tests de diagnostic d'intérêt taxonomique
Provedor de dados: 5
Autores: Duperthuy, Marylise.
Les bactéries du genre Vibrio représentent le groupe le plus fréquemment associé à des maladies infectieuses chez les mollusques marins et notamment l'huître creuse Crassostrea gigas. A ce jour trois espèces de Vibrio pathogènes d'huîtres creuses V. splendidus, V. aestuarianus et récemment V. coralliitycus ont été identifiés en France. La prévention des mortalités associées à ces vibrioses repose sur une meilleure compréhension des interactions hôte-pathogène et sur l'utilisation de techniques de diagnostic rapides à mettre en oeuvre et fiables. Ce travail a permis d'une part de caractériser la dynamique de phagocytose de vibrions par les cellules immunocompétentes de l'huître, les hémocytes. La mise au point d'un protocole d'analyse en cytométrie de flux...
Tipo: Text
Palavras-chave: Huître creuse; Crassostrea gigas; Vibrio splendidus; Vibrio aestuarianus; Vibrio corralliitycus; Hémocytes; Phagocytose; Cytométrie de flux; Diagnostic; PCRq.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/00033/14452/11753.pdf

Effect of a mono-specific algal diet on immune functions in two bivalve species - Crassostrea gigas and Ruditapes philippinarum
Provedor de dados: 5
Autores: Delaporte, Maryse; Soudant, Philippe; Moal, Jeanne; Lambert, Christophe; Quere, Claudie; Miner, Philippe; Choquet, Gwenaelle; Paillard, Christine; Samain, Jean-francois.
The impact of diets upon the fatty acid composition of haemocyte polar lipids and consequently upon immune parameters has been tested in the oyster Crassostrea gigas and the clam Ruditapes philippinarum. Oysters and clams were fed each of three cultured algae: Chaetoceros calcitrans, which is rich in 20:5(n-3) and 20:4(n-6) and poor in 22:6(n-3) fatty acids; T-Iso (Isochrysis sp.), which is rich in 22:6(n-3) and deficient in 20:5(n-3) and 20:4(n-6); and Tetraselmis suecica, which is deficient in 22:6(n-3) and contains only small amounts of 20:5(n-3) and 20:4(n-6). Fatty acid composition of haemocyte polar lipids was greatly affected by the diet. Oysters and clams fed C. calcitrans maintained a higher proportion of 20:5(n-3) and 20:4(n-6) in their haemocyte...
Tipo: Text
Palavras-chave: Ruditapes philippinarum; Crassostrea gigas; Polyunsaturated fatty acids; Phagocytosis; Oxidative activity; Haemocyte membrane; Nutrition; Immunology; Bivalve; Immunologie; Bivalve; Ruditapes philippinarum; Crassostrea gigas; Acides gras poly insatures; Phagocytose; Flambee respiratoire; Membrane; Hemocyte; Nutrition.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-354.pdf