Consulta: subjectFacets:"Phagocytosis"
Registros recuperados: 49
Data/hora: 10/06/2024 13:57:52

FISH IMMUNE SYSTEM
Provedor de dados: 72
Autores: Olabuenaga,Susana E.
The defence against infections is the essential function of the immune system of all Vertebrates. Fish present an efficient and developed immunologic response and in the case of Teleosts, with some similarities to the higher vertebrates. It can be divided into two types: innate or non specific and adquired or specific, both with a humoral and cellular compartments. Their relative importance can change according to the age of fish and is always under the influence of different factors. The most relevant components are mentioned here. There are two factors in fishes which strongly influence the immune response and antibody production, seasonal changes and temperature. The main lymphoid organs are described here: the thymus, the kidney and the spleen, as well...
Tipo: Journal article
Palavras-chave: Antigen; Antibody; Leukocytes; Phagocytosis; Lymphokines; Inflammation; Immunostimulants.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200010

Effects of extracellular products from the pathogenic Vibrio aestuarianus strain 01/32 on lethality and cellular immune responses of the oyster Crassostrea gigas
Provedor de dados: 5
Autores: Labreuche, Yannick; Soudant, Philippe; Goncalves, Madeleine; Lambert, Christophe; Nicolas, Jean-louis.
Vibrio aestuarianus strain 01/32 was previously shown to be pathogenic to Crassostrea gigas juveniles. To investigate virulence mechanisms of this pathogen, we studied the toxicity to oysters of its extracellular products (ECPs). ECPs displayed lethality to animals, with a LD50 value of 3.3 mu g/g body weight. To determine the oyster cellular immune responses induced by these ECPs, we further examined in vitro their effects on C. gigas hemocytes, using flow cytometric-based hemocyte assays. Treatment of hemolymph with ECPs caused a significant inhibition of hemocyte phagocytosis and adhesive capabilities. In contrast, the pathway of reactive oxygen species production was enhanced by higher ECP concentrations. Exposure of hemocytes to live bacteria induced...
Tipo: Text
Palavras-chave: Vibrio aestuarianus; ECPs; Crassostrea gigas; ROS production; Adhesion; Phagocytosis; Flow cytometry; Bivalve immunity.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1035.pdf

Immune response of the Antarctic sea urchin Sterechinus neumayeri: cellular, molecular and physiological approach
Provedor de dados: 5
Autores: Gonzalez-aravena, Marcelo; Perez-troncoso, Carolina; Urtubia, Rocio; Branco, Paola; Machado Cunha Da Silva, Jose Roberto; Mercado, Luis; De Lorgeril, Julien; Bethke, Jorn; Paschke, Kurt.
In the Antarctic marine environment, the water temperature is usually between 2 and - 1.9 degrees C. Consequently, some Antarctic species have lost the capacity to adapt to sudden changes in temperature. The study of the immune response in Antarctic sea urchin (Sterechinus neumayeri) could help us understand the future impacts of global warming on endemic animals in the Antarctic Peninsula. In this study, the Antarctic sea urchins were challenged with lipopolysaccharides and Vibrio alginolitycus. The cellular response was evaluated by the number of coelomocytes and phagocytosis. A significant increase was observed in red sphere cells and total coelomocytes in animals exposed to LPS. A significant rise in phagocytosis in animals stimulated by LPS was also...
Tipo: Text
Palavras-chave: Sea urchin; Antarctica; Sterechinus neumayeri; Coelomocytes; Phagocytosis; Gene expression.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00439/55012/56441.pdf

Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat
Provedor de dados: 5
Autores: Luna-acosta, Andrea; Renault, Tristan; Thomas-guyon, Helene; Faury, Nicole; Saulnier, Denis; Budzinski, Helene; Le Menach, K.; Pardon, P.; Fruitier-arnaudin, I.; Bustamante, Paco.
In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially hostile environment, i.e. to an herbicide alone or within a mixture. Following 2 h of exposure to the herbicide diuron at 1 mu g L-1 the repression of different genes implicated in immune defence mechanisms in the haemocytes and the inhibition of enzyme activities, such as laccase-type phenoloxidase (PO) in the plasma, were observed. The inhibition of superoxide dismutase (SOD) activity in the plasma was also observed after 6 and 24 h of exposure. In the mixture with the herbicides...
Tipo: Text
Palavras-chave: Bivalve; Pollution; Gene expression; Laccase; Superoxide dismutase; Phagocytosis.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00083/19435/17475.pdf

Fine cytochemical localization of lysosomal organelles in phagocytic cells of bivalve molluscs.
Provedor de dados: 5
Autores: Auffret, Mathieu; Diouris, M; Herry, A.
Bivalve molluscs possess several types of hemolymph cells (hemocytes), involved in internal defense and other physiological functions. Studies in pathological and experimental conditions have shown their micro- and macrophagic ability. However, the mechanisms of intracellular digestion are incompletely known in these organisms. The lysosomal apparatus of hemocytes was therefore investigated in several species by hydrolase localization with the electron microscope. The cytoplasmic granules of granulocytes displayed a strong acid beta -glycerophosphatase activity. Previous observations had shown that these granules may contain phagocytized material. This indicates that intracellular digestion is achieved in these organelles. Acid beta -glycerophosphatase...
Tipo: Text
Palavras-chave: Bivalvia; Phagocytosis; Cells; Ultrastructure; Haemolymph.
Ano: 1991
URL: http://archimer.ifremer.fr/doc/1991/acte-1631.pdf

Modulation des paramètres hémocytaires par la nutrition de l'huitre creuse Crassostrea gigas. Implication dans les mortalités estivales
Provedor de dados: 5
Autores: Delaporte, Maryse.
In the context of Pacific oyster C. gigas summer mortality, the present study demonstrated that the reproductive effort reduced not only the energetic parameters (carbohydrates and CEA) but also the immune parameters (concentration, phagocytosis and adhesive capacity of hemocytes). Increasing food supply from 4% to 12% (of oyster dry weight) did not counteract this phenomena as most of the additional energy was dedicated to the gametogenesis processes. However, after the gametogenesis, the 12% diet clearly favored energy storage restoration and hemocyte production. Hemocyte parameters were also modulated by the food quality. Indeed, hemocyte concentration tended to increase 20:5n-3 and/or 20:4n-6 were supplied by micro-algae or artificial diets. When these...
Tipo: Text
Palavras-chave: Summer mortalities; Flow cytometer; Immunity; Oxidative burst; Phagocytosis; Biochemical composition hemocyte parameters; Essential fatty acids; Nutrition; Crassostrea gigas; Mortalités estivales; Cytomètre de flux; Immunité; Poussée respiratoire; Phagocytose; Réponses hémocytaires; Composition biochimique; Acides gras essentiels; Nutrition; Crassostrea gigas.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/these-357.pdf

Comparison of haemocytic parameters among flat oyster Ostrea edulis stocks with different susceptibility to bonamiosis and the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Comesana, Pilar; Casas, Sandra M.; Cao, Asuncion; Abollo, Elvira; Arzul, Isabelle; Morga, Benjamin; Villalba, Antonio.
Farming of the flat oyster Ostrea edulis in Europe is severely constrained by the protozoan Bonamia ostreae. The introduction of the resistant species Crassostrea gigas has been a relief for the farmers, while the pilot programmes to select O. edulis strains resistant to bonamiosis performed in various countries can be seen as a promising strategy to minimise the effects of bonamiosis. However, the physiological bases of this differential susceptibility remain unknown. A search for an explanation of the intra and interspecific differences in oyster susceptibility to bonamiosis was accomplished by comparing some immune parameters among various O. edulis stocks and C gigas. On December 2003, naive and Bonamia-relatively resistant flat oysters from Ireland....
Tipo: Text
Palavras-chave: Ostrea edulis; Crassostrea gigas; Bonamia ostreae; Haemocyte; Phagocytosis; Respiratory burst; Nitric oxide.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00074/18501/16092.pdf

Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Duchemin, M; Auffret, Mathieu; Thomas-guyon, Helene; Renault, Tristan.
Parameters of hemocyte populations have been considered as relevant indicators of bivalve health and are currently used in immunotoxicological studies. Hemocytes in hemolymph can be collected by puncturing either the pericardial cavity or the adductor muscle sinus with a syringe. Flow cytometry is a methodological approach that is increasingly being used in laboratories for the study of hemocyte parameters in aquatic invertebrates. However, various protocols for hemocyte processing in laboratories equipped with different types of cytometers have been published. In this context, two flow cytometers ( EPICS XL4 (R), Beckman Coulter and FacsCalibur (R), Becton Dickinson) and two sites of hemocyte collection ( pericardial cavity and adductor muscle sinus) were...
Tipo: Text
Palavras-chave: Crassostrea gigas; Phagocytosis; Hemocytes; Flow cytometry.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-3898.pdf

Etude des amibes marines et de leurs interactions avec les vibrios pathogènes d’huître
Provedor de dados: 5
Autores: Robino, Etienne.
Free living amoebae inhabit aquatic environments and use phagocytosis of bacteria for their nutrition. According to the hypothesis of coincidental evolution of virulence, the cellular and molecular mechanisms of phagocytosis being preserved from amoebae to the immune cells of animals, the predation exerted by amoebae could favor the emergence of pathogenic bacteria resistant to phagocytosis. Since 2008, Crassostrea gigas oysters have suffered from over-mortality in France. This poly-microbial disease involves the Herpes OsHV-1 μvar virus which causes an immunosuppression of oysters that are then colonized by various opportunistic pathogenic bacteria including vibrios inducing the death of the animal. V. tasmaniensis LGP32 is a facultative intracellular...
Tipo: Text
Palavras-chave: Amibes libres; Vibrios pathogènes; Phagocytose; Évolution fortuite; Diversité; Free-Living amoebae; Pathogenic vibrios; Phagocytosis; Coincidental evolution; Diversity.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00692/80419/83555.pdf

Bonamia ostreae, parasite de l'huître plate, Ostrea edulis: sa position taxonomique parmi les parasites du groupe "microcell", analyses des interactions hôte/parasite chez plusieurs populations d'huîtres plates
Provedor de dados: 5
Autores: Besnard-cochennec, Nathalie.
Bonamia ostreae is the causative agent of hemocyte disease. It has been detected first during mortalities occured in Franc in 1979. Bonamiosis spread rapidly among oysters farming in France and Europ. Another species, Bonamia sp. has been detected in New Zealand and Australia. These paasites were both included in the "microcell" group. This group contains 2 others parasites, Mikrocytos mackini affecting Crassostrea gigas in British Columbia (Canada) and M. roughleyi affecing Saccostrea glomerata in Australia. Structural and molecular caracterisation of all these parasites were realised. Results show tha B. ostreae, B. sp., B. exitiosus. Molecular tools are available for genus detection, Bonamia spp. and Mikrocytos and species identification (B. ostreae, B....
Tipo: Text
Palavras-chave: Bonamia ostreae; Ostrea edulis; Taxonomie; Diagnotic; PCR; In situ hybridation; Hémocytes; Lectines; Phagocytose; Résistance; Bonamia ostreae; Ostrea edulis; Taxonomy; Diagnotic; PCR; In situ hybridization; Hemocytes; Lectins; Phagocytosis; Resistance.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00211/32223/30671.pdf

Combination of a pesticide exposure and a bacterial challenge: In vivo effects on immune response of Pacific oyster, Crassostrea gigas (Thunberg)
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Gay, Melanie; Huvet, Arnaud; Daniel, Jean-yves; Saulnier, Denis; Renault, Tristan.
To assess the impact of pollution induced by pesticides on Pacific oyster, Crassostrea gigas, health in France, in vivo effects of combined pesticide exposure and bacterial challenge on cell activities and gene expression in hemocytes were tested using flow cytometry and real-time PCR. As a first step, an in vivo model of experimental contamination was developed. Pacific oysters were exposed to a mixture of eight pesticides (atrazine, glyphosate, alachlor, metolachlor, fosetyl-alumimium, terbuthylazine, diuron and carbaryl) at environmentally relevant concentrations over a 7-day period. Hemocyte parameters (cell mortality, enzyme activities and phagocytosis) were monitored using flow cytometry and gene expression was evaluated by real-time PCR (RT-PCR)....
Tipo: Text
Palavras-chave: Gene expression; Flow cytometry; Vibrio splendidus; Pathogenesis; Phagocytosis; Hemocyte; Pesticides; Bivalve immunity; Crassostrea gigas; Pacific oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3022.pdf

Impact of food availability on energy storage and defense related hemocyte parameters of the Pacific oyster Crassostrea gigas during an experimental reproductive cycle
Provedor de dados: 5
Autores: Delaporte, Maryse; Soudant, P; Lambert, C; Moal, Jeanne; Pouvreau, Stephane; Samain, Jean-francois.
The aim of this study was to test the effect of food quantity on energy storage and defense capacities of oysters during a reproductive cycle. One-year-old Crassostrea gigas oysters were fed two different dietary rations (4% and 12% of oyster dry weight in algal dry weight per day) in controlled experimental conditions over an annual cycle. Oyster dry weights, carbohydrate and lipid contents, energetic adenylate charge, and hemocyte parameters of oysters were significantly affected by reproductive processes related to seasonal temperature variation and, to a lesser extent, by the dietary rations. Energy parameters decreased during gametogenesis as gonads developed then increased during the gonad resorption phase. The additional energy provided to oysters...
Tipo: Text
Palavras-chave: Reactive oxygen species production; Reproduction; Phagocytosis; Hemocyte parameters; Energy storage; Crassostrea gigas.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1549.pdf

Characterization of abalone Haliotis tuberculata-Vibrio harveyi interactions in gill primary cultures
Provedor de dados: 5
Autores: Pichon, Delphine; Cudennec, Benoit; Huchette, Sylvain; Djediat, Chakib; Renault, Tristan; Paillard, Christine; Auzoux-bordenave, Stephanie.
The decline of European abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vitro studies on the interactions between abalone H. tuberculata and V. harveyi. This work reports the development of primary cell cultures from abalone gill tissue, a target tissue for bacterial colonisation, and their use for in vitro study of host cell—V. harveyi interactions. Gill cells originated from four-day-old explant primary cultures were successfully sub-cultured in multi-well plates and maintained in vitro for up to 24 days....
Tipo: Text
Palavras-chave: Haliotis tuberculata; Vibrio harveyi; Gill cell culture; Pathogenicity; Phenoloxidase; Phagocytosis.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00146/25683/23745.pdf

Etude des effets de polluants sur les paramètres hémocytaires de l'huître creuse, Crassostrea gigas - Interactions entre environnement, mécanismes de défense et maladies infectieuses
Provedor de dados: 5
Autores: Gagnaire, Beatrice.
Shellfish industry is mostly realized in estuary zones, which are subjected to pollutions due to anthropic activities. The harmful effects of pollutants on animals inhabiting these estuarine zones are poorly known. Among these animals, Pacific oyster, Crassostrea gigas, may represent a model because they are sedentary and they filter water intensively. Among all physiological functions possibly disturbed by pollutants, defence mechanisms are poorly studied in bivalves. Moreover, animals presenting impaired defence mechanisms may be more sensitive to infectious diseases. In this context, effects of pollutants on hemocyte functions and on sensitiveness to diseases were tested in C. gigas. After adjusting the protocols for the monitoring of hemocyte...
Tipo: Text
Palavras-chave: Real time PCR; Flow cytometry; Pesticides; Pollutants; Phagocytosis; Hemocytes; Crassostrea gigas; Pacific oyster; Pathology; Immunotoxicology; PCR en temps réel; Cytométrie de flux; Pesticides; Polluants; Phagocytose; Hémocytes; Crassostrea gigas; Huître creuse; Pathologie; Immunotoxicologie.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/these-730.pdf

Immune priming and portal of entry effectors improve response to vibrio infection in a resistant population of the European abalone
Provedor de dados: 5
Autores: Dubief, Bruno; Nunes, Flavia; Basuyaux, Olivier; Paillard, Christine.
Since 1997, populations of the European abalone Haliotis tuberculata suffer mass mortalities attributed to the bacterium Vibrio harveyi. These mortalities occur at the spawning season, when the abalone immune system is depressed, and when temperatures exceed 17 °C, leading to favorable conditions for V. harveyi proliferation. In order to identify mechanisms of disease resistance, experimental successive infections were carried out on two geographically distinct populations: one that has suffered recurrent mortalities (Saint-Malo) and one that has not been impacted by the disease (Molène). Furthermore, abalone surviving these two successive bacterial challenges and uninfected abalone were used for several post-infection analyses. The Saint-Malo population...
Tipo: Text
Palavras-chave: Immunity; Hemocyte; Abalone; Disease; Extracellular products; Immune priming; Vibrio harveyi; Flow cytometry; Resistance; Phagocytosis; Bacterial growth; QPCR; Gill.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00357/46800/46667.pdf

Effect of in vivo pesticide exposure and injection of bacteria on immune gene expression in the Pacific oyster, Crassostrea gigas
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Renault, Tristan; Thomas Guyon, Hélène; Burgeot, Thierry.
Bay of Marennes-Oleron is an important French area of reproduction for sea bass, Dicentrarchus labrax. This area is often subjected to many pollutions due to anthropic activities, including pesticides and polycyclic aromatic hydrocarbons (PAH) which cause adverse effects in aquatic organisms. The harmful effects of such   contaminants on fish immune system leading to a decreased resistance against pathogens begin to be described, but remain poorly  known. In this context, influence of micropolluants, like PAH, on the defense mechanisms developed by sea bass was tested. Fish were exposed five days to a soluble fraction of PAH with environmental concentration (760 ng/L). At the end of the contamination, fish were transferred to non contaminated sea water and...
Tipo: Text
Palavras-chave: Phagocytosis; Vibrio; Pesticides; Pollutant; Crasssotrea gigas; Oysters.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/acte-3317.pdf

Changes of gill and hemocyte-related bio-indicators during long term maintenance of the vent mussel Bathymodiolus azoricus held in aquaria at atmospheric pressure
Provedor de dados: 5
Autores: Bettencourt, R; Dando, P; Rosa, D; Riou, V; Colaco, A; Sarrazin, Jozee; Sarradin, Pierre-marie; Santos, R.
The deep-sea hydrothermal vent mussel Bathymodiolus azoricus has been the subject of several studies aimed at understanding the physiological adaptations that vent animals have developed in order to cope with the particular physical and chemical conditions of hydrothermal environments. In spite of reports describing successful procedures to maintain vent mussels under laboratory conditions at atmospheric pressure, few studies have described the mussel's physiological state after a long period in aquaria. In the present study, we investigate changes in mucocytes and hemocytes in B. azoricus over the course of several months after deep-sea retrieval. The visualization of granules of mucopolysaccharide or glycoprotein was made possible through their inherent...
Tipo: Text
Palavras-chave: Vent; Hydrothermal vent; Bathymodiolus azoricus; Aquarium acclimatization; Phagocytosis; Alcian blue Periodic Acid Schiff staining; Mucopolysaccharide.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4357.pdf

Analysis of hemocyte parameters in Pacific oysters, Crassostrea gigas, reared in the field — Comparison of hatchery diploids and diploids from natural beds
Provedor de dados: 5
Autores: Gagnaire, Beatrice; Soletchnik, Patrick; Faury, Nicole; Kerdudou, Nolwenn; Le Moine, Olivier; Renault, Tristan.
Internal defence mechanisms of bivalves include the hemocytes which assume several functions. Relationships exist between environmental factors, physiological state of oysters, defence system and pathogens. Hatchery oysters are more and more produced and reared in the field. The characteristics and particularly the defence system of these animals are poorly documented. In this context, two field trials were carried out from May to September 2002 and from April to July 2003 in order to monitor hemocyte parameters of diploid oysters from natural beds and hatchery-produced diploid oysters using flow cytometry. Several hemocyte parameters (cell mortality, granulocyte percentage, phagocytosis, esterase and peroxidase activities) were monitored. For both oyster...
Tipo: Text
Palavras-chave: Hatchery; Natural beds; Diploids; Flow cytometry; Peroxidase activity; Esterase activity; Phagocytosis; Hemocytes; Crassostrea gigas; Pacific oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3119.pdf

Effect of a mono-specific algal diet on immune functions in two bivalve species - Crassostrea gigas and Ruditapes philippinarum
Provedor de dados: 5
Autores: Delaporte, Maryse; Soudant, Philippe; Moal, Jeanne; Lambert, Christophe; Quere, Claudie; Miner, Philippe; Choquet, Gwenaelle; Paillard, Christine; Samain, Jean-francois.
The impact of diets upon the fatty acid composition of haemocyte polar lipids and consequently upon immune parameters has been tested in the oyster Crassostrea gigas and the clam Ruditapes philippinarum. Oysters and clams were fed each of three cultured algae: Chaetoceros calcitrans, which is rich in 20:5(n-3) and 20:4(n-6) and poor in 22:6(n-3) fatty acids; T-Iso (Isochrysis sp.), which is rich in 22:6(n-3) and deficient in 20:5(n-3) and 20:4(n-6); and Tetraselmis suecica, which is deficient in 22:6(n-3) and contains only small amounts of 20:5(n-3) and 20:4(n-6). Fatty acid composition of haemocyte polar lipids was greatly affected by the diet. Oysters and clams fed C. calcitrans maintained a higher proportion of 20:5(n-3) and 20:4(n-6) in their haemocyte...
Tipo: Text
Palavras-chave: Ruditapes philippinarum; Crassostrea gigas; Polyunsaturated fatty acids; Phagocytosis; Oxidative activity; Haemocyte membrane; Nutrition; Immunology; Bivalve; Immunologie; Bivalve; Ruditapes philippinarum; Crassostrea gigas; Acides gras poly insatures; Phagocytose; Flambee respiratoire; Membrane; Hemocyte; Nutrition.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-354.pdf

Comparative Quantitative and Qualitative Attributes of the Surface Respiratory Macrophages in the Domestic Duck and the Rabbit
Provedor de dados: 77
Autores: Mutua,P. M; Gicheru,M. M; Makanya,A. N; Kiama,S. G.
In mammals, surface respiratory macrophages (SM) are known to play a foremost role in protecting the respiratory system by providing first line of defense through engulfing pathogens and particulate matter respired with air. It has been reported that the pulmonary cellular defense system of domestic birds is inadequate. In particular, low number of SM and even lack of the cells in a healthy avian respiratory system have been associated with susceptibility of domestic birds to respiratory diseases. In an endeavor to resolve the existing controversy, the quantitative and qualitative attributes of the surface respiratory macrophages of the domestic duck and of the domestic rabbit were compared under similar experimental conditions. Quantitatively, the rabbit...
Tipo: Journal article
Palavras-chave: Avian; Cellular defense; Lung; Phagocytosis; Rabbit; Surface macrophages.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200008

Effect of Bothrops alternatus snake venom on macrophage phagocytosis and superoxide production: participation of protein kinase C
Provedor de dados: 80
Autores: Setubal,SS; Pontes,AS; Furtado,JL; Kayano,AM; Stábeli,RG; Zuliani,JP.
Envenomations caused by different species of Bothrops snakes result in severe local tissue damage, hemorrhage, pain, myonecrosis, and inflammation with a significant leukocyte accumulation at the bite site. However, the activation state of leukocytes is still unclear. According to clinical cases and experimental work, the local effects observed in envenenomation by Bothrops alternatus are mainly the appearance of edema, hemorrhage, and necrosis. In this study we investigated the ability of Bothrops alternatus crude venom to induce macrophage activation. At 6 to 100 ¼g/mL, BaV is not toxic to thioglycollate-elicited macrophages; at 3 and 6 ¼g/mL, it did not interfere in macrophage adhesion or detachment. Moreover, at concentrations of 1.5, 3, and 6 ¼g/mL...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops; Macrophages; Phagocytosis; Superoxide; Protein kinase C; Inflammation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400010

Bioactivities of extracts from the marine sponge Halichondria panicea
Provedor de dados: 80
Autores: Purushottama,GB; Venkateshvaran,K; Pani Prasad,K; Nalini,P.
In the present study, we screened the biological activity of extracts from the marine sponge Halichondria panicea collected in the Arabian Sea. Crude toxin was obtained by methanol, chloroform-methanol (2:1) and aqueous extraction. Subsequently, the protein concentration of each crude extract was determined. The impact of both sponge methanolic and aqueous extracts was found to increase activities of Na+-K+ ATP-ase and Mg++ ATP-ase. In the case of chloroform-methanol extract, higher concentrations increased acetylcholine esterase (AchE) activity. The methanolic and chloroform-methanol extracts exhibited hemolytic activity on chicken and human erythrocytes, whereas the aqueous extract failed to do so. Methanol and aqueous extracts produced an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Halichondria panicea; Phagocytosis; Neuromodulation; Hemolysis; Angiogenesis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300007

Depletion of Phagocytic Cells during Nonlethal Plasmodium yoelii Infection Causes Severe Malaria Characterized by Acute Renal Failure in Mice
Provedor de dados: 19
Autores: Terkawi, Mohamad Alaa; Nishimura, Maki; Furuoka, Hidefumi.
In the current study, we examined the effects of depletion of phagocytes on the progression of Plasmodium yoelii 17XNL infection in mice. Strikingly, the depletion of phagocytic cells, including macrophages, with clodronate in the acute phase of infection significantly reduced peripheral parasitemia but increased mortality. Moribund mice displayed severe pathological damage, including coagulative necrosis in liver and thrombi in the glomeruli, fibrin deposition, and tubular necrosis in kidney. The severity of infection was coincident with the increased sequestration of parasitized erythrocytes, the systematic upregulation of inflammation and coagulation, and the disruption of endothelial integrity in the liver and kidney. Aspirin was administered to the...
Palavras-chave: Phagocytes; Macrophages; Phagocytosis; Plasmodium yoelii.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4479

An Acute-phase Protein as a Regulator of Sperm Survival in the Bovine Oviduct: Alpha 1-acid-glycoprotein Impairs Neutrophil Phagocytosis of Sperm In Vitro
Provedor de dados: 19
Autores: LIU, Jinghui; A. MAREY, Mohamed; KOWSAR, Rasoul; HAMBRUCH, Nina; SHIMIZU, Takashi; HANEDA, Shingo; MATSUI, Motozumi; SASAKI, Motoki; HAYAKAWA, Hiroyuki; PFARRER, Christiane; MIYAMOTO, Akio; 羽田, 真悟; 松井, 基純; 佐々木, 基樹; 宮本, 明夫.
We have previously shown that polymorphonuclear neutrophils (PMNs) are present in bovine oviduct fluid under physiological conditions, and that the oviduct provides a microenvironment that protects sperm from phagocytosis by PMNs. Alpha 1-acid glycoprotein (AGP) is a major acute-phase protein produced mainly in the liver that has immunomodulatory functions. AGP mRNA is expressed in extrahepatic organs, such as the lung, kidney, spleen, lymph node, uterus, and ovary. Therefore, in this study, we investigated, 1) the local production of AGP in the bovine oviduct, 2) the effect of AGP on the phagocytic activity of PMNs for sperm and superoxide production and 3) the impact of AGP desialylation on the PMN phagocytosis of sperm. The AGP gene was expressed in...
Palavras-chave: Alpha 1-acid glycoprotein (AGP); Bovine; Oviduct; Phagocytosis; Sperm.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3946

Endothelin-1 downregulates sperm phagocytosis by neutrophils in vitro: a physiological implication in bovine oviduct immunity
Provedor de dados: 19
Autores: MAREY, Mohamed Ali; YOUSEF, Mohamed Samy; LIU, Jinghui; MORITA, Kazuhiro; SASAKI, Motoki; HAYAKAWA, Hiroyuki; SHIMIZU, Takashi; ELSHAHAWY, Ibrahim I.; MIYAMOTO, Akio; 佐々木, 基樹; 清水, 隆; 宮本, 明夫.
The oviduct is an active contractile tube that provides the proper environment for sperm transport, capacitation and survival. Oviductal contractions are regulated by autocrine/paracrine secretion of several factors, such as prostaglandins (PGs) and endothelin-1 (EDN-1). We have previously shown that during the preovulatory stage, sperm are exposed to polymorphonuclear neutrophils (PMNs) in the bovine oviduct, and the bovine oviduct epithelial cells (BOECs) secrete molecules including PGE2 that suppress sperm phagocytosis by PMNs in vitro. In this study, we investigated the possible effects of EDN-1 on the phagocytic activity of PMNs toward sperm. The local concentrations of EDN-1 in oviduct fluid and BOEC culture medium ranged from 10–10 to 10–11 M as...
Palavras-chave: Endothelin-1; Oviduct; Phagocytosis; Polymorphonuclear neutrophils (PMN); Sperm.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4310

Bovine S protein (vitronectin) increases phagocytosis of Streptococcus dysgalactiae
Provedor de dados: 59
Autores: Filippsen,Laerte Francisco.
The effects of bovine S protein (vitronectin) on phagocytosis of Streptococcus dysgalactiae strains isolated from cattle with mastitis were investigated. Phagocytized streptococci were determined by a fluorometric microassay using glass adherent polymorphonuclear neutrophils (PMN). Preincubation of S. dysgalactiae with bovine S protein significantly increased their phagocytosis by PMN. Bovine S protein had no effect on phagocytic killing of non-S protein binding S. pyogenes cultures. Enzymatic digestion of the bovine S protein binding sites on S. dysgalactiae with pronase resulted in a significative reduction of the effects of S protein on phagocytosis. It could thus be concluded that in addition to its role as a promoter of cellular adhesion and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus dysgalactiae; Bovine S protein; Phagocytosis; Bovine mastitis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100003

Hemócitos fagocitários em larvas de Diatraea saccharalis (Fabricius) (Lepidoptera, Pyralidae)
Provedor de dados: 104
Autores: Falleiros,Angela M.F; Gregório,Elisa A.
The ultrastructural observation of the hemocytes of D. saccharalis larvae showed different hemocyte tvpes in various stage of interaction with microorganisms, which were interpreted as bacteria. The microorganisms were observed either free in the hemolymph or into the insect hemocytes. The bacteria were detected mostly into lhe granulocytes and plasmatocytes: the spherule cells and the oenocytoids also presented intracytoplasmic microorganisms, but less frequently. The fagocytic capacity of the different hemocyte tvpes is discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lepidoptera; Pyralidae; Diairaea saccharalis; Hemocyte; Phagocytosis; Ultrastructure.
Ano: 1995
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751995000400004

Dietary lipid sources affect freshwater catfish jundiá, Rhamdia quelen, survival, when challenged with Aeromonas hydrophila - doi: 10.4025/actascianimsci.v35i4.19617
Provedor de dados: 2
Autores: Vargas, Rodrigo Javier; Universidade Federal de Santa Catarina (UFSC); Dotta, Geovana; Universidade Federal de Santa Catarina (UFSC); Mouriño, José Luiz; Universidade Federal de Santa Catarina (UFSC); Silva, Bruno Correia da; Universidade Federal de Santa Catarina (UFSC); Fracalossi, Débora Machado; Universidade Federal de Santa Catarina (UFSC).
The immune modulatory activity of diet long chain polyunsaturated fatty acids in fish has been previously demonstrated, although results for freshwater species are controversial.  This study evaluates the effect of different dietary lipids on freshwater catfish jundiá, Rhamdia quelen, survival and its non-specific response (phagocytosis) after being inoculated with the pathogen  Aeromonas hydrophila. Five diets were offered to jundiá fingerlings during 150 days prior to challenge: cod liver oil (FO), sunflower oil (SO), linseed oil (LO), canola oil (CO) and coconut oil (CNO). Accumulated mortality was significantly lower in fish fed FO and CNO diets and higher in fish fed LO. In spite of the highest values observed for phagocytotic activity in CNO-fed fish...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.06.03.00-0 mortality; Phagocytosis; Oils; Immune response Mortality; Phagocytosis; Oils; Immune response.
Ano: 2013
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/19617

Acute inflammatory response in Nile tilapia fed probiotic Lactobacillus plantarum in the diet - doi: 10.4025/actascibiolsci.v33i3.8011
Provedor de dados: 3
Autores: Dotta, Geovana; Laboratório AQUOS-Sanidade de Organismos Aquáticos, Depto. de Aqüicultura, CCA, UFSC - Rod. Admar Gozaga 1346 - 88040-900 - Florianópolis, SC - Brasil; Mouriño, José Luis Pedreira; Laboratório de Camarões Marinhos, Depto. de Aqüicultura, CCA, UFSC, Beco dos Coroas, Barra da Lagoa - Florianópolis, SC - Brasil; Jatobá, Adolfo; Laboratório de Camarões Marinhos, Depto. de Aqüicultura, CCA, UFSC, Beco dos Coroas, Barra da Lagoa - Florianópolis, SC - Brasil; Burgos Morán, Ricardo Ernesto; Universidad Politécnica de Catalunya, Campus del Baix Llobregat, Av. del Canal Olímpic, s/n - 08860 - Castelldefels, Catalunya - España; Pilati, Celso; UFSC; Martins, Maurício Laterça; UFSC.
The present study evaluated the acute inflammatory response induced by carrageenin (500 &amp;micro;g) injected in the swim bladder of Nile tilapia, after fed or not probiotic supplemented diet. Fifty four fish were distributed in six treatments and three replicates: Group A: Fish fed unsupplemented diet: 0.5 mL saline-injected fish; fish injected with 500 &amp;micro;g carrageenin diluted in 0.5 mL saline; Non-injected. Group B: Fish fed probiotic supplemented diet: saline-injected fish; carrageenin-injected fish; Non-injected. Fifteen days after feeding the fish were injected with carrageenin or saline. After six hours, inflammatory exudate was collected, as well as the blood for hematocrit, red blood cell (RBC) and white blood cell (WBC) counts,...
Tipo: Composição bioquímica
Palavras-chave: 2.00.00.00-6 Ciencias Biologicas Tilapia; Probiotic; Lactobacillus plantarum; Inflammation; Haematology; Phagocytosis 2.00.00.00-6 Ciencias Biologicas Tilapia; Probiotic; Lactobacillus plantarum; Inflammation; Haematology; Phagocytosis.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8011

Iron transportation across the placenta
Provedor de dados: 42
Autores: Oliveira,Claudia M. de; Rodrigues,Marcio N.; Miglino,Maria Angélica.
According to the classification of placental types among animals, the transfer of iron through the placenta can occur via: absorption connected to transferin through the outer surface of the trophoblast in direct contact with circulating maternal blood; absorption of the erythrocytes by the chorionic epithelium in direct contact with accumulation of blood extravased from haemotophagous areas; absorption by the chorionic epithelium in direct contact with iron enriched secretions from the endometrial glands and absorption by extravasations of the blood in the maternal-fetal surface and the subsequent phagocytosis of the erythrocytes by trophoblast cells described in bovine, small ruminants, canine and feline. The function of erythrophagocytosis observed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endocitosis; Iron; Phagocytosis; Placental hematoma.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000400025

Influence of orchiectomy of seven month old bulls on bronchoalveolar immune function
Provedor de dados: 45
Autores: Bertagnon,H.G.; Batista,C.F.; Santos,B.P. dos; Lima,M.G.B.; Bellinazzi,J.B.; Libera,A.M.M.P. Della.
ABSTRACT The present study evaluated the impact that orchiectomy, a routine but painful intervention in bovine husbandry, can cause on pulmonary immunity. To identify whether orchiectomy can impair lung defense, analyses of serum cortisol concentration and of alveolar macrophage and their function (phagocytosis and respiratory burst) were evaluated. Sixteen Holstein bulls (7 mo old, 250±50kg of body weight BW) were divided in two homogeneous groups - the castrated group and the sham group - and the sample were collected on Days -7, 1 and 7 relative to the day of the procedure. Serum cortisol concentration statistically increased on Days 1 and 7 (D-7: 4,97±1,28ng/ml; D1: 6,35 ±1,10ng/ml; D7: 8,28±1,94ng/ml) after castration and these variables seem to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Cortisol; Innate immunity; Phagocytosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000200310

Increased leishmanicidal activity of alveolar macrophages from mature horses with mild equine asthma
Provedor de dados: 45
Autores: Lessa,D.A.B.; Alencar,N.X.; Torres Filho,R.A.; Costa,M.F.M.; Fernandes,W.R.; Saraiva,E.M.
ABSTRACT Alveolar macrophages (AMs) are an essential part of defense mechanisms within the lungs and their phagocytic activity is important for organ homeostasis. The phagocytic ability of AMs obtained from bronchoalveolar lavage from 17 mature mixed-breed pleasure horses (8 healthy and 9 diagnosed with mild equine asthma) was studied through assays with Leishmania (Viannia) braziliensis promastigotes, which enabled the calculation of a phagocytic index (PI) and a survival index (SI). Results indicate that phagocytic activity of AMs in asthma affected horses is similar to healthy horses, while leishmanicidal activity is significantly increased in horses with asthma.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Horses; Equine asthma; Alveolar macrophage; Phagocytosis; Leishmania (Viannia) braziliensis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300939

Probiotic technological and functional characteristics of Lactobacillus strains isolated from chicken gut
Provedor de dados: 45
Autores: Dornas,R.M.; Silva,G.A.X.; Martins,C.H.V.; Moreira,J.L.S.; Jensen,C.E.M.; Souza,M.R.; Nicoli,J.R.; Nunes,A.C.; Neumann,E.
Lactobacillus spp. isolated from different portions of chickens' gastrointestinal tract were evaluated concerning their ability to survive in a water-in-oil (W/0) emulsion containing sesame and sunflower oil. After sixty days of emulsion storage under refrigeration, three of five strains tested survived in number equal to or higher than 10(6)cfu/g. Lactobacillus reuteri 2M14C, which presented the highest survival in W/O emulsion (10(7)cfu/g), was tested for its capacity to resist throughout the passage through gnotobiotic mice gastrointestinal tract and for the ability to stimulate murine peritoneal macrophages phagocytosis. This strain remained at a number above 10(9)cfu/g feces during ten days of monoassociation, and monoassociated mice showed phagocytic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken; Probiotic; Lactobacillus; Emulsion; Phagocytosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000100014

Defense reactions of Dermatobia hominis (Diptera: Cuterebridae) larval hemocytes
Provedor de dados: 47
Autores: Faraldo,Ana Carolina; Lello,Edy.
The defense reactions against biological (Histoplasma capsulatum and Escherichia coli) and non-biological materials (China ink and nylon thread) were tested in vivo in third instar larvae of Dermatobia hominis. The cellular defense performed by larval hemocytes was observed under electron microscopy. China ink particles were phagocytosed by granular cells 5 h after injection. E. coli cells were internalized by granular cells as early as 5 min after injection and totally cleared 180 min post-injection, when many hemocytes appeared disintegrated and others in process of recovering. H. capsulatum yeasts provoked, 24 h after being injected, the beginning of nodule formation. Nylon thread was encapsulated 24 h after the introduction into the hemocoel. Our...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dermatobia Hominis; Hemocytes; Phagocytosis; Nodule Formation; Encapsulation.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000200005

Performance and macrophage activity of broilers fed with a sorghum meal with different yeast wall levels
Provedor de dados: 52
Autores: Ferreira,Suelen Regina; Murakami,Alice Eiko; Silveira,Thais Gomes Verzignassi; Santos,José Maurício Gonçalves dos; Fernandes,Jovanir Inês Muller.
The aim of this work was to evaluate the effect of broiler breeders' age and yeast wall (YW) levels on broilers' performance and macrophage activitys. The experiment consisted in a completely randomized design and a 2 X 5 factorial arrangement and two controls of two broiler breeders age (34 and 57 weeks of age) and five YW levels (zero, one, two, three or four kg of YW/ton of diet). They received sorghum diet compared to a control corn/soybean meal diet. The age of the broiler breeders influenced the performance, but did not affect macrophage activity. At 34 weeks age broiler breeders' progeny, all diets were similar considering the average weight. For 57 weeks age broiler breeders' progeny, at 21 days, only average weight of sorghum diet supplemented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carcass yield; Mananoligossacarydes; Phagocytosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200019

Evaluation of possible failure of the mononuclear phagocyte system after total splenectomy in rats
Provedor de dados: 52
Autores: Marques,Ruy Garcia; Petroianu,Andy; Oliveira,Márcia Betânia Nunes de; Bernardo-Filho,Mário; Portela,Margareth Crisóstomo.
Young and adult Wistar rats were submitted to total splenectomy and compared to animals not submitted to any surgical manipulation in order to evaluate the phagocytic function of spleen. The animals were infected with Escherichia coli labeled with technetium-99m and killed 20 minutes later. Liver, lung, spleen and a blood clot sample were taken. No significant differences were found in the percentage of bacterial radioactivity uptake in mononuclear phagocyte system (MPS) organs in young and adult splenectomized rats. However, phagocytosis index by macrophages of MPS organs was smaller in splenectomized animals than in control group. Splenectomized rats were associated with a higher blood bacterial radioactivity uptake than animals of the control group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spleen; Splenectomy; Technetium-99m; Escherichia coli; Phagocytosis; Mononuclear phagocyte system.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200006

Role of previous stimulation on the neutrophil migration protects against the lethal effect of Salmonella typhimurium
Provedor de dados: 62
Autores: MORAES,Flávio Ruas de; KLEIN Jr.,Manoel Henrique; MORAES,Julieta Rodini Engrácia de.
The intraperitoneal inoculation of 3.8 x 10(9) CFU of alive Salmonella typhimurium caused 100% mortality in rats. In these animals a small number of neutrophils migrated to the peritoneal cavity. The intraperitoneal inoculation of the animals with thioglycollate (Tg, 90 mg), carrageenin (Cg, 500 mu g), or killed S. typhimurium (KS, 1.9 x 10(9) CFU) 24 hours before challenge with alive S. typhimurium caused a significant neutrophil migration to the peritoneal cavity (determined 6 hours after challenge) and protected the animals against the lethal effect of the bacteria at a rate of 50%, 83% and 80%, respectively. However, similar treatment of the animals with brain heart infusion broth (BHI, S. typhimurium grout medium) protected the animals by only 23%....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Migration; Neutrophils; Salmonella typhimurium; Peritonitis; Septicemia; Phagocytosis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000100002

Phagocytosis and killing of epidemic methicillin-resistant Staphylococcus aureus by human neutrophils and monocytes
Provedor de dados: 55
Autores: Salgado,M.M.; Pignatari,A.C.C.; Bellinati-Pires,R.
Staphylococcus aureus is a pathogen that has been associated with nosocomial infections since the preantibiotic era. Since the introduction of antibiotics in medical practice in the 1940 s, methicillin-resistant S. aureus (MRSA) strains have been emerging in various parts of the world. In view of the important role of the phagocytic system in the defense against this bacteria, we decided to study phagocytosis by neutrophils and monocytes of an epidemic MRSA strain in São Paulo, Brazil, in comparison with methicillin-sensitive strains. Complement system opsonins are fundamental for efficient ingestion of the resistant and sensitive strains by both types of phagocytes. We found no association of the opsonic requirement of the MRSA strain with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Human phagocytes; Phagocytosis; Epidemic methicillin-resistant.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000100005

Phagocytosis by macrophages mediated by receptors for denatured proteins - dependence on tyrosine protein kinases
Provedor de dados: 56
Autores: Hespanhol,M.R.; Mantovani,B.
Previous studies have demonstrated that some components of the leukocyte cell membrane, CR3 (Mac-1, CD11b/CD18) and p150/95, are able to bind to denatured proteins. Thus, it is of interest to know which effector functions of these cells can be triggered by these receptors when they interact with particles or surfaces covered with denatured proteins. In the present study we analyzed their possible role as mediators of phagocytosis of red cells covered with denatured bovine serum albumin (BSA) by mouse peritoneal macrophages. We observed that a) macrophages are able to recognize (bind to) these red cells, b) this interaction can be inhibited by denatured BSA in the fluid phase, c) there is no phagocytosis of these particles by normal macrophages, d)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phagocytosis; Denatured proteins; Macrophage; SS2 Integrins; Tyrosine protein kinases.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300015

MFG-E8, a clearance glycoprotein of apoptotic cells, as a new marker of disease severity in chronic obstructive pulmonary disease
Provedor de dados: 56
Autores: Zhang,S.; Xie,J.G.; Su,B.T.; Li,J.L.; Hu,N.; Chen,J.; Luo,G.W.; Cui,T.P.
Milk fat globule epidermal growth factor 8 (MFG-E8) is an opsonin involved in the phagocytosis of apoptotic cells. In patients with chronic obstructive pulmonary disease (COPD), apoptotic cell clearance is defective. However, whether aberrant MFG-E8 expression is involved in this defect is unknown. In this study, we examined the expression of MFG-E8 in COPD patients. MFG-E8, interleukin (IL)-1β and transforming growth factor (TGF)-β levels were measured in the plasma of 96 COPD patients (93 males, 3 females; age range: 62.12±10.39) and 87 age-matched healthy controls (85 males, 2 females; age range: 64.81±10.11 years) using an enzyme-linked immunosorbent assay. Compared with controls, COPD patients had a significantly lower plasma MFG-E8 levels (P&lt;0.01)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; COPD; MFG-E8; Phagocytosis; Smoking.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001101032

Zymosan phagocytosis by mouse peritoneal macrophages is increased by apoHDL- and not by intact HDL-covered particles
Provedor de dados: 56
Autores: Carvalho,M.D.T.; Tobias,V.E.; Vendrame,C.M.V.; Shimabukuro,A.F.M.; Gidlund,M.; Quintão,E.C.R.
The uptake of lipids and lipoprotein particles by macrophages undergoes phagocytic activation and the formation of foam cells are key events in atherosclerosis. In this study we determined how intact high density lipoproteins (HDL) and apolipoproteins-HDL (removal of the lipid component from HDL, i.e., apoHDL) influence the phagocytosis of zymosan by mouse peritoneal macrophages. Zymosan particles preincubated together with lipoproteins or alone (control) were incubated with the macrophages. Phagocytosis activity was reported as the percent of macrophages that internalized three or more zymosan particles. HDL co-incubated with zymosan did not influence the over-all uptake of zymosan particles compared to apoHDL, which greatly enhanced the ability of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HDL; ApoHDL; Zymosan particles; Atherosclerosis; Phagocytosis; Macrophages.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300009

Fluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell lines
Provedor de dados: 56
Autores: Teixeira,R.C.; Baêta,B.A.; Ferreira,J.S.; Medeiros,R.C.; Maya-Monteiro,C.M.; Lara,F.A.; Bell-Sakyi,L.; Fonseca,A.H.
This study aimed to describe the association of Borrelia burgdorferi s.s. with ixodid tick cell lines by flow cytometry and fluorescence and confocal microscopy. Spirochetes were stained with a fluorescent membrane marker (PKH67 or PKH26), inoculated into 8 different tick cell lines and incubated at 30°C for 24 h. PKH efficiently stained B. burgdorferi without affecting bacterial viability or motility. Among the tick cell lines tested, the Rhipicephalus appendiculatus cell line RA243 achieved the highest percentage of association/internalization, with both high (90%) and low (10%) concentrations of BSK-H medium in tick cell culture medium. Treatment with cytochalasin D dramatically reduced the average percentage of cells with internalized spirochetes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Borrelia burgdorferi; Tick cell lines; Phagocytosis; Fluorescent membrane marker.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000700601

Influence of acute renal failure on the mononuclear phagocytic system
Provedor de dados: 56
Autores: Sousa,V.R.A.; Sousa,A.A.; Petroianu,A.; Simal,C.J.R.; Barbosa,A.J.A.
Several studies show the ability of macrophages to remove particles injected into the bloodstream. This function seems to be increased in the presence of acute renal failure. The objective of the present study was to assess the phagocytic function of the main organs (spleen, liver and lung) of the mononuclear phagocytic system in renal and postrenal failures. Fifteen rats (250-350 g) were divided into three groups (N = 5): group I - control; group II - ligature of both ureters, and group III - bilateral nephrectomy. On the third postoperative day, all animals received an iv injection of 1 ml/kg 99mTc sulfur colloid. Blood samples were collected for the assessment of plasma urea, creatinine, sodium, and potassium concentrations and arterial gasometry....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renal failure; Reticuloendothelial system; Phagocytosis; Macrophage; Scintigraphy.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900010

Human monocytes tolerant to LPS retain the ability to phagocytose bacteria and generate reactive oxygen species
Provedor de dados: 56
Autores: Fernandes,M.L.; Mendes,M.E.; Brunialti,M.K.C.; Salomão,R.
Tolerance to lipopolysaccharide (LPS) occurs when animals or cells exposed to LPS become hyporesponsive to a subsequent challenge with LPS. This mechanism is believed to be involved in the down-regulation of cellular responses observed in septic patients. The aim of this investigation was to evaluate LPS-induced monocyte tolerance of healthy volunteers using whole blood. The detection of intracellular IL-6, bacterial phagocytosis and reactive oxygen species (ROS) was determined by flow cytometry, using anti-IL-6-PE, heat-killed Staphylococcus aureus stained with propidium iodide and 2',7'-dichlorofluorescein diacetate, respectively. Monocytes were gated in whole blood by combining FSC and SSC parameters and CD14-positive staining. The exposure to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IL-6; ROS; Phagocytosis; MALP-2; Flagellin; LPS tolerance.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900008

Role of Tamm-Horsfall protein in the binding and in vivo phagocytosis of type 1 fimbriated Escherichia coli by mouse peritoneal macrophages
Provedor de dados: 56
Autores: Bastos,A.C.S.C.; Santos,L.B.; Tamashiro,W.M.S.C.; Yamada,A.T.; Oliveira,U.M.; Yano,T.
Tamm-Horsfall glycoprotein (THP) contains manno-oligosaccharides that are recognized by type 1 fimbriae (F1) of Escherichia coli. In the present study, we examined the in vivo phagocytic activity of mouse peritoneal macrophages after treatment of bacteria with THP. At low THP concentrations (12.5 µg/ml and 50 µg/ml) no significant difference was observed in the phagocytosis of E. coli F1+. However, at high THP concentrations (500 µg/ml and 1250 µg/ml) we obtained a reduction of bacterial phagocytosis by mouse peritoneal macrophages.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tamm-Horsfall protein; Type 1 fimbriated E. coli; Phagocytosis.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700011

Modulation of peritoneal macrophage activity by the saturation state of the fatty acid moiety of phosphatidylcholine
Provedor de dados: 56
Autores: Grando,F.C.C.; Felício,C.A.; Twardowschy,A.; Paula,F.M.; Batista,V.G.; Fernandes,L.C.; Curi,R.; Nishiyama,A.
To determine the effects of saturated and unsaturated fatty acids in phosphatidylcholine (PC) on macrophage activity, peritoneal lavage cells were cultured in the presence of phosphatidylcholine rich in saturated or unsaturated fatty acids (sat PC and unsat PC, respectively), both used at concentrations of 32 and 64 µM. The treatment of peritoneal macrophages with 64 µM unsat PC increased the production of hydrogen peroxide by 48.3% compared to control (148.3 ± 16.3 vs 100.0 ± 1.8%, N = 15), and both doses of unsat PC increased adhesion capacity by nearly 50%. Moreover, 64 µM unsat PC decreased neutral red uptake by lysosomes by 32.5% compared to the untreated group (67.5 ± 6.8 vs 100.0 ± 5.5%, N = 15), while both 32 and 64 µM unsat PC decreased the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phosphatidylcholine; Lecithin; Macrophages; Fatty acids; Reactive oxygen species; Phagocytosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700003

Treatment of serum with supernatants from cultures of Candida albicans reduces its serum-dependent phagocytosis
Provedor de dados: 58
Autores: Santos,Aderbal Antonio dos; Sá,Eglius Alexandre Colignese de; Gaziri,Luis Carlos Jabur; Felipe,Ionice.
Candida albicans is a potent activator of the complement system, and heat labile opsonins produced by activation of C3 (C3b and iC3b) enhance phagocytosis of C. albicans mediated by complement receptors. In this study we treated mouse serum with supernatants from cultures of a protease producer strain of C. albicans and evaluated the ability of this serum to enhance phagocytosis of C. albicans. Cell-free supernatants from cultures of C. albicans were concentrated 5 fold and added to mouse serum for 30 min at 37ºC, before using this serum for opsonization of glutaraldehyde-fixed yeast cells. We observed that normal mouse serum increased about 3 fold the phagocytosis of C. albicans by mice peritoneal macrophages, whereas supernatant-treated serum did not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candida albicans; Phagocytosis; Proteinase.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100016

Identification and biological activity of potential probiotic bacterium isolated from the stomach mucus of breast-fed lamb
Provedor de dados: 58
Autores: Sepová,H. Kiňová; Dubničková,M; Bilková,A; Bukovský,M; Bezáková,L.
The lactic acid bacterium E isolated from the stomach mucus of breast-fed lamb was identified by sequencing of 16S rDNA fragment and species-specific PCR as Lactobacillus reuteri. Its potential antimicrobial activity and ability to modulate immune system in vitro and in vivo was determined. The growth inhibition of potential pathogens decreased from Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica ser. Minnesota to Escherichia coli. The lowest inhibition activity was observed in the case of Candida albicans. The ability of L. reuteri E to modulate biological activities of human and mouse mononuclear cells was estimated in vitro and in vivo, respectively. The production of IL-1β by monocytes in vitro was significantly induced by L. reuteri...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactobacillus reuteri; Mononuclear cells; PCR; Antagonistic activity; Phagocytosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300044

WBC count and functional changes induced by co-administration of clofazimine and clarithromycin, in single and multiple doses, in Wistar rats
Provedor de dados: 60
Autores: Paina,Flávia Aparecida; Miranda,Julise Cunha; Tavares,Cristiane Fernandes de Freitas; Queiroz,Regina Helena da Costa; Souza,Ana Maria de.
Clofazimine and clarithromycin are used to treat leprosy and infections caused by Mycobacterium avium complex. Little data on the toxicity of co-administration of these two drugs are available. Here we evaluated the potential adverse effects of polytherapy with these two drugs in male Wistar rats by determining WBCs counts and other blood cell counts, neutrophilic phagocytosis, and burst oxidative, by flow cytometry. We observed an increase in WBCs, in multiple-dose regimens, and in polymorphonuclear cells, in both single- clarithromycin only and multiple dose regimens. We also observed a reduction in mononuclear cell counts in single and multiple doses. The drugs seem to reverse the mononuclear and polymorphonuclear cell ratio. An increase in oxidative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clofazimine/adverse effects; Clofazimine/experimental study; Clarithromycin/adverse effects; Clarithromycin/experimental study; Leprosy/treatment; Mycobacterium avium/infections; Mycobacterium avium/treatment; Leukocytes; Phagocytosis; Oxidative burst.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000300008