Consulta: subjectFacets:"Phenotypic characterization"
Registros recuperados: 3
Data/hora: 10/06/2024 01:45:50

Genetic diversity of Gossypium barbadense from the central Brazilian Amazon
Provedor de dados: 36
Autores: HOFFMANN,Lúcia Vieira; CARDOSO,Kálita Cristina Moreira; ROCHA,Antônio Sabino Neto da Costa; OLIVEIRA,Aryanny Irene Domingues de; ABREU,Aluana Gonçalves; PEREIRA,Caio César de Oliveira; MALAFAIA,Guilherme; MENEZES,Ivandilson Pessoa Pinto de.
ABSTRACT The Amazon Basin is a center of diversity of Gossypium barbadense and the strategy for conservation of this genetic resource depends on the knowledge of the diversity maintained in Amazonas State. During two expeditions, in 2012 and 2014, plants were collected in ten municipalities in the state of Amazonas, in the central Brazilian Amazon region. The molecular diversity was estimated by SSR markers for 50 samples collected in 2012. The morphological diversity of 24 plants collected in 2014 was assessed ex situ and compared to that of 50 plants of the same and other cotton varieties from other Brazilian states. Most of plants evaluated in situ in Amazonas had purple petioles and veins (82%), associated to medicinal use, and kidney seeds (78%). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Genetic resources; Cotton tree; Phenotypic characterization.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672018000100001

Rat dental pulp stem cells: isolation and phenotypic characterization method aiming bone tissue bioengineering
Provedor de dados: 52
Autores: Bertassoli,Bruno Machado; Costa,Emanuela Silva; Sousa,Cristiane Aparecida; Albergaria,Juliano Douglas Silva; Maltos,Kátia L. Melo; Goes,Alfredo Miranda; Matins,Thais Maria da Mata; Silva,Gerluza Aparecida Borges; Jorge,Erika Cristina.
ABSTRACT: Dental pulp stem cells (DPSC) have been showing a considerable potential for regenerative medicine. Pulps were collected from lower incisors (n=2) through direct access of the tooth pulp chamber. The isolated cells were cultured in alfa-MEM 10% FBS, in standard culture conditions. At the third passage, DPSC were characterized by flow cytometry (MHCI, CD54, CD73, CD90, CD45, CD11 and CD34); RT-PCR for Nanog gene; and their differentiation capacity in osteogenic, adipogenic and chondrogenic cell lines. Isolated cells exhibited adhesion capacity to plastic; fusiform morphology, and 80% confluence reached in approximately 3 days. These cells have also revealed positive expression for CD54, CD73 and CD90 markers; and negative expression for CD11, CD34...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mesenchymal stem cells; Dental pulp; Murine; Phenotypic characterization; Cellular differentiation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100335

Phenotypic characterization and species-specific PCR of promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented sausages
Provedor de dados: 58
Autores: Sawitzki,Maristela Cortez; Fiorentini,Ângela Maria; Brod,Fábio Cristiano Angonesi; Tagliari,Caroline; Bertol,Teresinha Marisa; Arisi,Ana Carolina Maisonnave; Sant'Anna,Ernani Sebastião.
The purpose of the present work was to characterize promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented artisanal sausage manufactured in the northwestern region of Rio Grande do Sul state, Brazil. From 127 isolates of homofermentative, Gram-positive and catalase-negative lactic acid bacteria, ten isolates were randomly selected and the phenotypic characterization and species-specific PCR were performed. Genomic DNA from each isolated strain and from the reference strains L. plantarum ATCC 8014 and L. pentosus ATCC 8041 were amplified using two pairs of L. plantarum species-specific primers (16/Lpl and LbP11/LbP12). The results of the phenotypic characterization and species-specific PCR indicated that five out of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fermented sausage; L. plantarum; Phenotypic characterization; PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300031