Consulta: subjectFacets:"Phenotypic heterogeneity Bioreporter Flagellum expression"
Registros recuperados: 1
Data/hora: 11/06/2024 11:02:48

Rapid and efficient protocol to introduce exogenous DNA in Vibrio harveyi and Pseudoalteromonas sp.
Provedor de dados: 5
Autores: Delavat, Francois; Bidault, Adeline; Pichereau, Vianney; Paillard, Christine.
Vibrio campbellii BAA-1116 is renowned for its bioluminescence properties, and genetic tools are available to genetically track this strain. However, many other ecologically important V. harveyi strains exist, for which only few genetic tools are available. In this study, a rapid electroporation protocol was developed to transform replicative plasmids in various environmental V. harveyi and Pseudoalteromonas strains. Moreover, a mini-Tn7 delivery system was modified to site-specifically integrate mini-transposons in the genome of V. harveyi ORM4. As a proof-of-principle, replicative plasmids carrying bioreporters were introduced by electroporation in V. harveyi ORM4 cells, and gene expression was followed at the single cell level. We could demonstrate that...
Tipo: Text
Palavras-chave: Electroporation Mini-transposon Vibrio harveyi Pseudoalteromonas; Phenotypic heterogeneity Bioreporter Flagellum expression.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00459/57035/58934.pdf