Consulta: subjectFacets:"Plant mitochondria"
Registros recuperados: 4
Data/hora: 16/06/2024 17:22:04

RNA editing in plant mitochondria, cytoplasmic male sterility and plant breeding.
Provedor de dados: 69
Autores: Araya,Alexandre; Zabaleta,Eduardo; Blanc,Valérie; Bégu,Dominique; Hernould,Michel; Mouras,Armand; Litvak,Simon.
RNA editing in plant mitochondria is a post-transcriptional process involving the partial change of C residues into U. These C to U changes lead to the synthesis of proteins with an amino acid sequence different to that predicted from the gene. Proteins produced from edited mRNAs are more similar to those from organisms where this process is absent. This biochemical process involves cytidine deamination. The cytoplasmic male sterility (CMS) phenotype generated by the incompatibility between the nuclear and the mitochondrial genomes is an important agronomical trait which prevents inbreeding and favors hybrid production. The hypothesis that RNA editing leads to functional proteins has been proposed. This hypothesis was tested by constructing transgenic...
Tipo: Journal article
Palavras-chave: Plant mitochondria; RNA editing; Cytidine deamination; Cytoplasmic male sterility (CMS; Fertility restoration.
Ano: 1998
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000100004

Improved method for isolation of coupled mitochondria of Araucaria angustifolia (Bert.) O. Kuntze
Provedor de dados: 52
Autores: Mariano,André Bellin; Kovalhuk,Leonardo; Valente,Caroline; Maurer-Menestrina,Juliana; Pereira-Netto,Adaucto Bellarmino; Guerra,Miguel Pedro; Carnieri,Eva Gunilla Skare.
A method for the isolation of coupled mitochondria from the callus of Araucaria angustifolia is described for the first time. Mitochondria were isolated from embryogenic callus of A. angustifolia. They were metabolically active, able to sustain oxidative phosphorylation as shown by respiratory control ratio values, which were about 2.4 when respiring on succinate as substrate. Oxygen uptake experiments, using freeze-thawed disrupted mitochondria, showed the presence of alternative rotenone-insensitive NAD(P)H dehydrogenases, which were stimulated by Ca2+. The procedure now described for the isolation of A. angustifolia mitochondria is an important new tool, allowing the investigation of mitochondrial bioenergetics and metabolism and physiology of plants.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Araucaria angustifolia; Callus; Plant mitochondria; Respiratory chain.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600006

Important amino acid residues of potato plant uncoupling protein (StUCP)
Provedor de dados: 56
Autores: Jezek,P.; Costa,A.D.T.; Vercesi,A.E.
Chemical modifications were used to identify some of the functionally important amino acid residues of the potato plant uncoupling protein (StUCP). The proton-dependent swelling of potato mitochondria in K+-acetate in the presence of linoleic acid and valinomycin was inhibited by mersalyl (Ki = 5 µM) and other hydrophilic SH reagents such as Thiolyte MB, iodoacetate and 5,5'-dithio-bis-(2-nitrobenzoate), but not by hydrophobic N-ethylmaleimide. This pattern of inhibition by SH reagents was similar to that of brown adipose tissue uncoupling protein (UCP1). As with UCP1, the arginine reagent 2,3-butadione, but not N-ethylmaleimide or other hydrophobic SH reagents, prevented the inhibition of StUCP-mediated transport by ATP in isolated potato mitochondria or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant mitochondria; Uncoupling protein; Chemical modification; Mitochondrial swelling; Reconstitution.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200003

Activity and functional interaction of alternative oxidase and uncoupling protein in mitochondria from tomato fruit
Provedor de dados: 56
Autores: Sluse,F.E.; Jarmuszkiewicz,W.
Cyanide-resistant alternative oxidase (AOX) is not limited to plant mitochondria and is widespread among several types of protists. The uncoupling protein (UCP) is much more widespread than previously believed, not only in tissues of higher animals but also in plants and in an amoeboid protozoan. The redox energy-dissipating pathway (AOX) and the proton electrochemical gradient energy-dissipating pathway (UCP) lead to the same final effect, i.e., a decrease in ATP synthesis and an increase in heat production. Studies with green tomato fruit mitochondria show that both proteins are present simultaneously in the membrane. This raises the question of a specific physiological role for each energy-dissipating system and of a possible functional connection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant mitochondria; Alternative oxidase; Uncoupling protein; Regulation; Electron partitioning.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300002