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An Improved Protocol for Flow Cytometry Analysis of Phytoplankton Cultures and Natural Samples
Provedor de dados: 5
Autores: Marie, Dominique; Rigaut-jalabert, Fabienne; Vaulot, Daniel.
Preservation of cells, choice of fixative, storage, and thawing conditions are recurrent issues for the analysis of phytoplankton by flow cytometry. We examined the effects of addition of the surfactant Pluronic F68 to glutaraldehyde-fixed photosynthetic organisms in cultures and natural samples. In particular, we examined cell losses and modifications of side scatter (a proxy of cell size) and fluorescence of natural pigments. We found that different marine phytoplankton species react differently to the action of Pluronic F68. In particular, photosynthetic prokaryotes are less sensitive than eukaryotes. Observed cell losses may result from cell lysis or from cell adhesion to the walls of plastic tubes that are commonly used for flow cytometry analysis....
Tipo: Text
Palavras-chave: Phytoplankton; Flow cytometry; Preservation; Pluronic F68; Glutaraldehyde.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00374/48493/48858.pdf