Consulta: subjectFacets:"Pollen viability"
Registros recuperados: 23
Data/hora: 02/06/2024 05:11:09

Successful crosses between fungal-resistant wild species of Arachis (section Arachis) and Arachis hypogaea
Provedor de dados: 74
Autores: Fávero,Alessandra Pereira; Santos,Rodrigo Furtado dos; Simpson,Charles E.; Valls,José Francisco Montenegro; Vello,Natal Antonio.
Peanut (Arachis hypogaea) is the fifth most produced oil crop worldwide. Besides lack of water, fungal diseases are the most limiting factors for the crop. Several species of Arachis are resistant to certain pests and diseases. This study aimed to successfully cross the A-genome with B-K-A genome wild species previously selected for fungal disease resistance, but that are still untested. We also aimed to polyplodize the amphihaploid chromosomes; cross the synthetic amphidiploids and A. hypogaea to introgress disease resistance genes into the cultivated peanut; and analyze pollen viability and morphological descriptors for all progenies and their parents. We selected 12 A-genome accessions as male parents and three B-genome species, one K-genome species,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peanut; Interspecific hybrids; Polyploidization; Colchicine; Pollen viability.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300353

Genetic and ecological niche modeling of Calydorea crocoides (Iridaceae): an endemic species of Subtropical Highland Grasslands
Provedor de dados: 74
Autores: de Alencar,Juliana Lustosa Matos; Kaltchuk-Santos,Eliane; Fachinetto,Juliana; Tacuatiá,Luana Olinda; Forni-Martins,Eliana Regina; Stiehl-Alves,Eudes Maria; de Souza-Chies,Tatiana Teixeira.
Abstract Evolutionary analyses have been widely used for evaluation of genetic diversity of natural populations and correlate these data to the fitness of the species, especially in the case of threatened species. Calydorea crocoides occurs in a restricted area at altitudes from 800 to 1500 m in southern Brazil and is considered endangered. A study assessing genetic diversity, cytogenetic features and ecological niche was performed aiming to characterize C. crocoides by multidisciplinary approaches. Molecular data highlighted that most of the total variation (76%; p &lt; 0.001) was found within populations and the parameters of genetic diversity were high at the species level (PPB = 98.97%; I = 0.4319; h = 0.2821). Gene flow (Nm) was estimated in 0.97...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic differentiation; ISSR markers; Pollen viability; Cytogenetic analyses; Restricted distributed species.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200327

Cytological and genome size data analyzed in a phylogenetic frame: Evolutionary implications concerning Sisyrinchium taxa (Iridaceae: Iridoideae)
Provedor de dados: 74
Autores: Burchardt,Paula; Souza-Chies,Tatiana T.; Chauveau,Olivier; Callegari-Jacques,Sidia M.; Brisolara-Corrêa,Lauís; Inácio,Camila Dellanhese; Eggers,Lilian; Siljak-Yakovlev,Sonja; de Campos,José Marcello Salabert; Kaltchuk-Santos,Eliane.
Abstract Sisyrinchium is the largest genus of Iridaceae in the Americas and has the greatest amount of cytological data available. This study aimed at investigating how genomes evolved in this genus. Chromosome number, genome size and altitude from species of sect. Viperella were analyzed in a phylogenetic context. Meiotic and pollen analyses were performed to assess reproductive success of natural populations, especially from those polyploid taxa. Character optimizations revealed that the common ancestor of sect. Viperella was probably diploid (2n = 2x =18) with two subsequent polyplodization events. Total DNA content (2C) varied considerably across the phylogeny with larger genomes detected mainly in polyploid species. Altitude also varied across the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ancestral chromosome number; DNA content; Meiotic behavior; Pollen viability; Polyploidy.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200288

Combining capacity and heterosis in eggplant hybrids under high temperatures
Provedor de dados: 75
Autores: Valadares,Ricardo de N; A Nóbrega,Danieli; Lima,Lilian B de; Silva,Jordana Antônia dos S; Santos,Ana Maria M dos; Melo,Roberto de A; Menezes,Dimas.
ABSTRACT  The objective of this work was to estimate the combinatorial capacity and heterosis of eggplant hybrids under high temperature conditions. Seven genitors, twelve hybrid combinations, originated from a partial diallel, and the Ciça F1 hybrid, as control, were evaluated. The experiment was conducted under greenhouse conditions in randomized block design with four replications, from April to December 2017. The assessed traits related to high temperatures were pollen viability (PV) and fruit fixation index (FFI); the morphoagronomic traits were number of fruits per plant (NFP), fruit weight (FWe), production per plant (PP), fruit length (FL), fruit width (FWi), fruit length/width ratio (FLWR) and plant height (PH). The variance analysis showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum melongena; Partial diallel; Fixation index of fruits; Pollen viability; Productivity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362019000300348

Pollen viability and meiotic irregularities in a collection of Stevia rebaudiana (Bertoni) Bertoni cultivated in Tucumán, Argentina
Provedor de dados: 46
Autores: Budeguer,C.J; Camadro,E.L; Erazzú,L.E.
Stevia rebaudiana (Bertoni) Bertoni (2n=2x=22) (Asteraceae family) is a species of economic value due to the presence of steviol glycosides in leaves -mainly stevioside and rebaudioside A- which are non-caloric sugars. In 2013, a collection of plants (genotypes) from four contrasting environments was established in Tucuman, Argentina, for evaluation under local conditions and, eventually, breeding purposes. As a first step, pollen viability and meiosis was studied in a sample of 56 plants. The percentage of pollen viability varied from medium (69.4%) to high (99.6%) in 52 of them, but from low (36.5%) to relatively low (51.5%) in the remaining four. The latter four plants also presented pollen grains of heterogeneous size, which were classified as n...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stevia; Pollen viability; Meiosis; Sporads.
Ano: 2018
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332018000200005

Biologia floral da candeia (Eremanthus erythropappus, Asteraceae)
Provedor de dados: 190
Autores: Vieira, Fábio de Almeida; Fajardo, Cristiane Gouvêa; Carvalho, Dulcinéia de.
O objetivo deste trabalho foi examinar e documentar a biologia floral e os polinizadores da candeia (Eremanthus erythropappus) em uma população natural, sendo estudados a antese, a morfologia floral, a receptividade estigmática, a viabilidade de pólen e os visitantes florais. As flores pequenas (10,17 mm de comprimento) são cor de rosa, hermafroditas e organizadas em densos capítulos (média = 29 flores). Foi observada alta porcentagem de pólen viável (77,25%) e relativamente pouca disponibilidade de néctar para os visitantes florais (0,63 μL). As abelhas Apis mellifera e Trigona sp. foram os visitantes mais frequentes. Os comprimentos dos botões, estiletes e flores variaram significativamente entre plantas.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antese; Viabilidade de pólen; Visitantes florais; Óleo essencial Recursos Florestais; Engenharia Florestal; Botânica; Reprodução Anthesis; Pollen viability; Floral visitors; Essential oil.
Ano: 2012
URL: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/402

Obtaining tetraploid plants of ruzigrass (Brachiaria ruziziensis)
Provedor de dados: 96
Autores: Timbó,Ana Luiza de Oliveira; Souza,Patrícia Nirlane da Costa; Pereira,Roselaine Cristina; Nunes,Juliane Dornellas; Pinto,José Eduardo Brasil Pereira; Souza Sobrinho,Fausto de; Davide,Lisete Chamma.
The objective of the present work was to induce chromosome doubling in genotypes of Brachiaria ruziziensis with potential for production of good-quality biomass and tolerance/resistance to stress caused by aluminum and spittlebugs. Germinated seeds of B. ruziziensis were immersed in 0.1% colchicine solution for 2 or 3 hours. Ploidy level was determined by flow cytometry and confirmed by chromosome count. Fertility of the tetraploid plants was assessed through evaluation of pollen viability by staining. There was no statistical difference with regard to seedling survival and number of duplicate seedlings between the treatments. Survival rate was 8%, of which 11.45% were tetraploid genotypes. The mean viability of pollen grains from the evaluated tetraploid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA content; Forage plant breeding; Number of chromosomes; Polyploidy induction; Pollen viability.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000300004

Storage of pollen and properties of olive stigma for breeding purposes
Provedor de dados: 124
Autores: Zambon,Carolina Ruiz; Silva,Luiz Fernando de Oliveira da; Pio,Rafael; Bianchini,Flávio Gabriel; Oliveira,Adelson Francisco de.
ABSTRACT In order to ensure success in controlled hybridizations in olive tree cultivation, the information on pollen viability and stigma receptivity is essential. The aim was to establish methodologies that increase the preservation of pollen viability and to establish the time to perform crossbreeds in hybridization studies with olive trees. Three experiments were performed with plants from the cultivar Arbequina, in Maria da Fé, MG, Brazil. In the first experiment, the description of the flower events was performed. In the second, anthers were desiccated in eppendorfs, being stored at three different conditions for pollen viability test: room temperature (27 ºC), refrigerator (8 ºC) and freezer (-10 ºC). In order to evaluate the in vitro germination,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Olea europaea L.; Pollen viability; Pollen grains; Hybridization.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902018000200291

‘URS Campestre’ seedless orange: a new mutant with female sterility
Provedor de dados: 63
Autores: Bender,Renar João; Santos,Rinaldo Pires dos; Guerra,Divanilde; Schwarz,Sergio Francisco; Bender,Stefan da Silveira.
ABSTRACT: Seedlessness in fruit is a trait that is much sought after by juice making industries. Close to the city of São Sebastião do Caí, in the state of Rio Grande do Sul (RS), Brazil, a new mutant orange originating from natural mutation was identified and selected as a seedless material. To determine the mechanisms involved in the absence of seeds, the reproductive structures of this new mutant by comparison with a Valencia sweet orange as control, a cultivar with a profusion of seeds, was analyzed in terms of meiotic behavior, meiotic index, pollen viability, in vitro germination, and ovule features to determine the grounds for seed absence. Other morphological analyzes allowed for visualizing the structures of normal appearance and size in both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetic analysis; Embryo sac abortion; Female sterility; Meiotic analysis; Pollen viability.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000500371

Influence of high temperature on the reproductive biology of dry edible bean (Phaseolus vulgaris L.)
Provedor de dados: 63
Autores: Silva,Daiana Alves da; Pinto-Maglio,Cecília Alzira Ferreira; Oliveira,Érica Cristina de; Reis,Raquel Luiza de Moura dos; Carbonell,Sérgio Augusto Morais; Chiorato,Alisson Fernando.
ABSTRACT: The aim of this study was to investigate the effect of heat stress on 12 bean genotypes through the analysis of their reproductive biology in terms of flowering, pollen viability, meiotic behavior, and production. Plants were grown in a climate chamber at 25-20 °C (day and night) and at a high temperature treatment 37-26 °C (day and night) from the vegetative (V4) development stage to physiological maturity. The experimental design was 2 × 12 factorial arrangement with six replications and the factors consisted of heat treatments and genotypes. In three replications, the number of newly opened flowers was checked daily. At physiological maturity, the following traits were evaluated: percentage of pod set, number of pods, number of viable seeds,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Common bean; Heat stress; Pollen viability; Meiosis; Seed yield.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000301100

Cytogenetic evaluation of chili ( Capsicum spp ., Solanaceae) genotypes cultivated in Valle del Cauca, Colombia
Provedor de dados: 109
Autores: Souza-Macedo,Viviane de; García-Dávila,Mario Augusto; Castro,Gisele Renata de; Garzón-Bautista,Yuri Marcela; Caetano,Creucí Maria.
Abstract The genus Capsicum comprises a large group of hot and sweet peppers, with different morphological varieties and degree of fruits pungency. Due to its active principles is widely used in cooking and in traditional medicine, being sold in large worldwide. In order to provide cytogenetic information which supporting plant breeding programs, this study aimed to analyze the meiotic behavior, chromosome number and pollen viability of the three species of greater economic importance of Capsicum (C. annuum, C. frutescens and C. chinense). Therefore, fruits and flower buds were collected from the Experimental Center of the Universidad Nacional de Colombia, campus Palmira- CEUNP to the corresponding mitotic, meiosis and pollen viability analysis. Plants...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome number; Meiotic behavior; Meiotic index; Plant genetic resources conservation; Ploidy level; Pollen viability.
Ano: 2017
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122017000400612

Floral and reproductive biology of Alcantarea nahoumii (Bromeliaceae), a vulnerable endemic species of the Atlantic Forest
Provedor de dados: 39
Autores: Bastos,Maria Josirene Souza Moreira; Bastos,Lucimário Pereira; Souza,Everton Hilo de; Soares,Taliane Leila; Morais,Daniel Vieira; Souza,Fernanda Vidigal Duarte de; Costa,Maria Angélica Pereira de Carvalho.
ABSTRACT Alcantarea nahoumii occurs exclusively in the state of Bahia, Brazil, and is classified as vulnerable due to deforestation and frequent fires in the region. Knowledge of floral and reproductive biology is fundamental to understanding ecological interactions, as well as the reproductive success of plant species. The objective of this study was to evaluate the floral and reproductive biology of A. nahoumii in an Atlantic Forest fragment with regard to phenology, pollen viability, stigma receptivity, pollination ecology and reproductive systems, all of which are important parameters for of the development of conservation strategies for the species. Anthesis is diurnal and heterogeneous, starting at 6:30 a.m. and lasting until 8:00 a.m. Highest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bromeliaceae; Floral visitors; Nectar; Pollen viability; Reproductive systems; Stigma receptivity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000400665

A holoparasitic plant severely reduces the vegetative and reproductive performance of its host plant in the Caatinga, a Brazilian seasonally dry forest
Provedor de dados: 39
Autores: Cruz Neto,Oswaldo; Leal,Inara Roberta; Santos,Jean Carlos; Lopes,Ariadna Valentina.
ABSTRACT Host-parasite interactions between plants may reduce the vegetative and reproductive performance of the host plant. Although it is well established that parasitic plants may negatively affect the metabolism and the number of vegetative/reproductive structures of their hosts, the effects of this interaction on the reproductive characteristics of the host plant are poorly understood. Here we document the interaction between Cuscuta partita (Convolvulaceae) and its main host, Zornia diphylla (Fabaceae), in the Caatinga of northeastern Brazil. We measured diverse reproductive/vegetative attributes of Z. diphylla in 60 plots randomly distributed in patches that were parasitized and not parasitized by C. partita. Both vegetative and reproductive...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Caatinga; Cuscuta partita; Floral traits; Haustoria; Holoparasitic plants; Plant-plant interaction; Pollen viability; Reproductive success; Zornia diphylla.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000100147

Comparative pollination role of stamens and breeding system in three species of Commelina (Commelinaceae) in Ile-Ife, Nigeria
Provedor de dados: 39
Autores: Oziegbe,Matthew; Akinlua,Isaiah Opeyemi; Olalekan,Adebiyi Abdul-hakeem.
This study investigated pollination mechanisms and breeding systems in five Commelina variants: two subspecies of Commelina diffusa (C. diffusa subsp. aquatica and C. diffusa subsp. montana, each having two staminodes); two variants of C. erecta (with three staminodes); and C. lagosensis (also with three staminodes). In all three species, the staminodes have six lobes but only two fertile lateral lobes. We collected field samples and cultivated new plants from stem cuttings. When the plants flowered, we evaluated floral morphology, floral biology and breeding systems, creating eight separate experimental treatments, involving spontaneous and hand pollination, as well as varying degrees of emasculation of the flowers. Although the pollen from the lateral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Commelina; Pollen viability; Stamen type; Autogamy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000300012

Stigmatic receptivity of peach flowers submitted to heat stress
Provedor de dados: 1
Autores: Carpenedo, Silvia; Raseira, Maria do Carmo Bassols; Franzon, Rodrigo Cezar; Byrne, David Hawkins; da Silva, João Baptista.
Because of climatic changes, the cultivation of temperate climate plants such as peach in subtropical climates has become a challenge. In these areas, temperatures exceeding 25°C often occur during the pre-flowering and flowering phases. The high temperature causes damages by acting during the early stages of pollen-pistil interaction processes. The objective of this work was to evaluate the stigmatic receptivity of peach flowers at 18°C and 30°C. The pollen adherence was evaluated as well as the germination and presence of pollen tubes in the transmitting tissue of the style. The genotypes responded differently to temperature. ‘Granada,’ ‘Diamante’, and ‘Sensação’ had a stigmatic receptivity that was less affected when flowers were exposed to the higher...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prunus persica; Pollen viability; Pollen tubes; Flowering.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42450

Pollen grain viability in accessions of Crotalaria juncea L. (FABACEAE)
Provedor de dados: 115
Autores: Coelho,Ana P. D.; Morais,Katiule P.; Dail Laughinghouse IV,Haywood; Giacomini,Sandro J.; Tedesco,Solange B.
Studies on pollen viability are useful for breeding Crotalaria juncea L. since they provide information that will increase the chance of successful intraspecific crossing. The aim of this study was to evaluate pollen viability in C. juncea accessions cultivated in Rio Grande do Sul, Brazil. Inflorescences of 10 accessions of C. juncea were collected, fixed in absolute ethanol: glacial acetic acid (3:1) for 72 h at room temperature and placed in 70 % ethanol. Analysis of pollen viability was performed with slides of anthers using the squashing technique and staining with acetic orcein 2 % and Alexanders stain. Means were compared using the Tukey test (p≤0.05). Pollen viability was high and significantly different among the accessions; the highest value was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalaria juncea Alexander's stain; Acetic orcein; Pollen viability; Plant cytogenetics.
Ano: 2012
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952012000500005

Botanical aspects of Heteropterys umbellata (Malpighiaceae): a cytological and palynological approach
Provedor de dados: 42
Autores: Sousa,Saulo M.; Reis,Aryane C.; Gomes,Shaiany S.L.; Bernardo,Karina B.; Salimena,Fátima R.G.; Viccini,Lyderson F.
The genus Heteropterys is one of the major genera in Malpighiaceae. However, few cytological and palynological studies were reported. The present work described for the first time the chromosome number, heterochromatin pattern, meiotic behavior, pollen viability and palynological aspects of Heteropterys ubellata, a very spread species. One large Brazilian population was evaluated using conventional techniques for meiotic studies and acetolyse to access the pollen morphology. The species showed 2n = 20 chromosomes, normal meiotic development and viable pollens. Great blocks of heterochromatin were observed around the centromers. DAPI staining was positive for centroeric heterochroatin, hile CMA3 ark as observed just at terinal regions of one pair of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome number; Heterochromatin pattern; Heteropterys umbellata; Malpighiaceae; Meiosis; Pollen viability.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000400009

Relationship between meiotic instability and fertility in F2 generation Arabusta coffee plants
Provedor de dados: 53
Autores: Granato,Laís Moreira; Ramos,Luis Carlos da Silva; Pinto-Maglio,Cecília Alzira Ferreira.
ABSTRACT Parental plants, an F1 interspecific hybrid and the F2 generation from Arabusta coffee plants were investigated for meiotic behavior and viability of pollen to understand part of their reproductive biology that affects their possible use in coffee breeding programs. Both parental plants (C. canephora var. Robusta 4x and C. arabica var. dihaploid Bourbon Vermelho) showed a meiosis diploid-like behavior, despite presenting a small percentage of irregularities, just as occurred for the F1 Arabusta hybrid. On the other hand, all F2 plants showeda higher frequency of anomalies that compromised pollen viability. The highest meiotic indices were registered for three analyzed plants of the F2 generation, and the pollen viability tests revealed the highest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsporogenesis; Meiotic index; Pollen viability; Post-meiotic products.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000200215

A putative mutant of a self-compatible yellow passion fruit with the corona color as a phenotypic marker
Provedor de dados: 53
Autores: Souza,Margarete Magalhães; Viana,Alexandre Pio; Pereira,Telma Nair Santana.
Yellow passion fruit (Passiflora edulis f. flavicarpa) is a self-incompatible crop with purple corona flowers (PCF). Plants exhibiting white corona flowers (WCF) were observed in an experimental field located in Campos dos Goytacazes - RJ, Brazil. This study was performed in order to test the fertility of these genotypes through studies of in vivo pollination, meiotic behavior and pollen viability. Using in vivo pollination, the mean fruit set percentage, obtained from self-pollinations at the moment that flowers open, was 70.83% in WCF and 0% in PCF. In terms of cytological analysis, for both kinds of plants, WCF and PCF, the meiotic behavior was considered regular, considering the high meiotic index estimated for both of them, 96.3% and 97.7%, and pollen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passion flower; Self-compatibility; In vivo pollination; Meiotic behavior; Pollen viability.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052010000100003

Cytogenetic studies in some species of Passiflora L. (Passifloraceae): a review emphasizing Brazilian species
Provedor de dados: 52
Autores: Souza,Margarete Magalhães; Pereira,Telma N. Santana; Vieira,Maria Lúcia Carneiro.
The Passifloraceae is represented by species of tropical and subtropical origin. The Passiflora is the richest genus with approximately 450 species, 200 of them being native to Brazil. Recent karyological studies have reported the basic chromosome number for the Passiflora genus as x = 6, whereas x = 9, x = 10 and x = 12 were established as secondary basic numbers. High rates of fertility occur in most Passiflora species, since both meiotic index and pollen viability are above 90%. Unusual meiotic behavior has been described in some taxa. Unviable pollen were observed in some diploids species. The genome size varies from 1.83 to 5.36 pg, and significant interspecific variance has been observed. Studies using the FISH methodology have shown that there are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; Banding; Meiotic behavior; Pollen viability; Nuclear DNA content; FISH.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200003

Pollen treatment in high osmotic potential: a simple tool for in vitro preservation and manipulation of viability in gametophytic populations
Provedor de dados: 61
Autores: Loguercio,Leandro Lopes.
A method for in vitro preservation and manipulation of pollen viability based on simple changes in osmotic potential (sucrose concentrations) in culture media was developed using tobacco pollen as the experimental model. High osmotic potentials were capable of reversibly inhibiting pollen germination, preserving its viability at room temperature for long periods, as assessed by subsequent incubation in germination medium. When pollen was pre-germinated for different periods and subsequently incubated in 80 % sucrose medium (inhibiting medium) there was a progressive decrease in its viability, which was a trend best described by a quadratic regression line. Nevertheless, very small variations in pollen-tube lengths and viable pollen grains more resistant to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gametophytic selection; In vitro pollen germination; Nicotiana tabacum; Pollen storage; Pollen viability.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000100009

Meiotic analysis of the germoplasm of three medicinal species from Asteraceae family
Provedor de dados: 65
Autores: Olkoski,Denise; Laughinghouse,Haywood Dail; Silva,Antonio Carlos Ferreira da; Tedesco,Solange Bosio.
Cytogenetic characterization was carried out on 12 accessions from Aster squamatus (Spreng.) Hieron., Pterocaulon polystachyum DC, and Solidago microglossa DC by studying their meiotic behavior and pollen viability. These species are from the Asteraceae family, native to Rio Grande do Sul State, Brazil, and are important for medicinal use. Young inflorescences with four accessions of each species were collected, fixed in ethanol-acetic acid (3:1), and conserved in ethanol 70% until use. The method used was that of squashing the anthers and coloring with acetic orcein 2%. Meiosis was regular in all accessions, presenting chromosomal associations preferentially bivalent, where n=10 was found for Aster squamatus and n=9 for Pterocaulon polystachyum, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asteraceae; Meiotic behavior; Pollen viability.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000600047

Determination of cardinal temperatures for sugar apple ( Annona squamosa L.)
Provedor de dados: 64
Autores: Rodrigues,Bruno Rafael Alves; Santos,Rayane Carneiro dos; Nietsche,Silvia; Mercadante-Simões,Maria Olívia; Cunha,Isabella Renata Gomes da; Pereira,Marlon Cristian Toledo.
ABSTRACT The sugar apple is highly dependent on the pollination and fruit tree pollen performance is crucial for successful fertilization and fruit set. The objective of this study was to determine the cardinal temperatures for in vitro pollen grains germination and evaluate the effect of temperature on pollen tube growth of sugar apple. The experimental design was in a completely randomized with eight treatments (10, 15, 20, 25, 30, 35, 40 and 45 ºC), four replicates and each plot was constituted by two slides per parcel. The cardinal temperatures were determined by evaluating in vitro germination of pollen grains and pollen tube growth in standard culture medium. It also assessed the pollen tube growth and the percentage of germination in vitro depending...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro germination; Pollen viability; Monads; Tetrads; Pollen tube.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542016000200145