Consulta: subjectFacets:"Polyhydroxyalkanoates"
Registros recuperados: 10
Data/hora: 06/06/2024 02:17:03

Biosynthesis of medium chain length poly-3-hydroxyalkanoates by Pseudomonas guezennei from various carbon sources
Provedor de dados: 5
Autores: Simon-colin, Christelle; Raguenes, Gerard; Costa, Bernard; Guezennec, Jean.
The biosynthesis of poly-(3-hydroxyalkanoates) (PHAs) by Pseudomonas guezennei using glucose and/or fatty acids with chain-length from 3 to 18 carbon atoms as carbon sources was investigated. Glucose, acetate, pyruvate, propionate, valerate, hexanoate, heptanoate, octanoate, decanoate, and oleic acid were supplemented in the mineral medium as the sole carbon source or as a mixture for PHAs accumulation. Chemical composition of the resulting PHAs was analysed by coupled gas chromatography mass spectroscopy (GCMS), Fourier Transform Infrared Spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR). PHAs contained up to 10 different types of 3-hydroxyalkanoic acid units, including saturated 3-hydroxyacids from C4 to C14, and unsaturated monomers...
Tipo: Text
Palavras-chave: Carbon substrates; Pseudomonas guezennei; Polymer characterization; Polyhydroxyalkanoates.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-6115.pdf

Orange and Passion Fruit Wastes Characterization, Substrate Hydrolysis and Cell Growth of Cupriavidus necator, as Proposal to Converting of Residues in High Value Added Product
Provedor de dados: 42
Autores: LOCATELLI,GABRIEL O.; FINKLER,LEANDRO; FINKLER,CHRISTINE L.L.
Abstract: Brazil is the world’s largest producer of orange and passion fruit, which are destined mainly for industrialization, generating grand volumes of wastes. The solid portion of these residues is a rich source of pectin - composed mainly of galacturonic acid and neutral sugars, which through the hydrolysis process can be used in biological conversion processes, as the production of polyhydroxyalkanoates (PHAs). This way, we characterized these wastes, followed by the extraction and hydrolysis of pectin for employ as a substrate for the cell growth of Cupriavidus necator. The results confirmed the large portion of pectin (almost 40 g.100g-1) and soluble sugars, present in these wastes. The hydrolyzed extract showed as a good source of carbon for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological conversion processes; Enzymatic hydrolyses; Orange wastes; Passion fruit wastes; Pectin; Polyhydroxyalkanoates.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100629

Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition
Provedor de dados: 52
Autores: Khan,Abdul Basit; Khattak,Mehmood Iqbal; Tarar,Omer Mukhtar; Habib,Farzana; Jamil,Khalid; Yasmin,Arfa; Parvez,Shoukat.
In this study, a variety of samples were screened for the presence of PHA synthase gene. Results showed that 16 out of 102 isolated were positive for PHA respective genes. The highest prevalence was observed in Pseudomonas aeruginosa. The capability of PHA production was also shown by growing these strains on the defined medium and subsequent analysis using intracellular granules staining and Fourier transform infrared spectroscopy (FT-IR). The microscopic analysis showed that the positive strains accumulated PHA in the cell. The FT-IR analysis showed the presence of PHA peaks in the dried cells as well as in extraction product. P aeruginosa strain P7 showed higher concentration of PHA compared to the others as demonstrated by the highest respective peaks...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyhydroxyalkanoates; Biodegradable polymers; PHA synthase gene; FTIR.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400015

Production of polyhydroxyalkanoates (PHAs) by Bacillus subtilis and Escherichia coli grown on cane molasses fortified with ethanol
Provedor de dados: 52
Autores: Gomaa,Eman Zakaria.
The aim of this work was to study the production of polyhydroxyalkanoates (PHAs) by Bacillus subtilis and Escherichia coli isolated from the industrial contaminated soil samples using cane molasses as an inexpensive substrate. The amount of PHA accumulated followed a similar pattern to its growth for each of treatment indicating a growth-related production, yielding maximum PHA production of 54.1 and 47.16% for B. subtilis and E. coli, respectively after 96 h cultivation in the medium containing 6 and 8 % molasses, respectively and decreased thereafter. The growth and PHA yields were improved by introducing 1% ethanol into the molasses medium. Ammonium sulphate and ammonium nitrate at a concentration of 1 g/L served as the best nitrogen sources for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyhydroxyalkanoates; Cane molasses; Infrared spectroscopy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100020

Microbial Synthesis and Characterization of Biodegradable Polyester Copolymers from Burkholderia Cepacia and Cupriavidus Necator Strains Using Crude Glycerol as Substrate
Provedor de dados: 52
Autores: Rodrigues,Plínio Ribeiro; Silvério,Tatiane Aparecida Barroso; Druzian,Janice Izabel.
Abstract Polymers are materials of pronounced importance in the modern world, since they are massively present in everyday life, especially in the form of packaging. However, most of these materials are derived from non-renewable sources and their disposal generates large volumes of waste, which is extremely damaging to natural environments. In this context, microbial biopolymers appear as a powerful alternative in the substitution of several applications of synthetic plastics, causing less harm to the environment, as they are biodegradable and produced from renewable sources. In this study, evaluation and characterization of polyhydroxyalkanoates (PHAs) produced by Cupriavidus necator (IPT 026 and IPT 027) and Burkholderia cepacia (IPT 119 and IPT 400),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crude glycerol; Biosynthesis; Polyhydroxyalkanoates; Characterization.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100209

Volatile fatty acids influence on the structure of microbial communities producing PHAs
Provedor de dados: 58
Autores: Ciesielski,Slawomir; Przybylek,Grzegorz.
Polyhydroxyalkanoates (PHAs) can be produced by microorganisms and are a biodegradable alternative to fossil-fuel based plastics. Currently, the focus is on reducing production costs by exploring alternative substrates for PHAs production, and on producing copolymers which are less brittle than monomers. Accordingly, this study used a substrate consisting of wastewater from waste-glycerol fermentation, supplemented with different amounts of acetic and propionic acids. These substrates were used to feed mixed microbial communities enriched from activated sludge in a sequencing batch reactor. A reactor supplemented with 2 mL of acetic acid produced 227.8 mg/L of a homopolymer of hydroxybutyrate (3HB); 4 mL of acetic acid produced 279.8 mg/L 3HB; whereas 4 mL...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biopolymers; Microbial community; Polyhydroxyalkanoates; RISA; Thauera sp.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200005

Variation analysis of bacterial polyhydroxyalkanoates production using saturated and unsaturated hydrocarbons
Provedor de dados: 58
Autores: Tufail,Saiqa; Munir,Sajida; Jamil,Nazia.
ABSTRACT Polyhydroxyalkanoates (PHA) are efficient, renewable and environment friendly polymeric esters. These polymers are synthesized by a variety of microbes under stress conditions. This study was carried out to check the suitability of waste frying oil in comparison to other oils for economical bioplastic production. Six bacterial strains were isolated and identified as Bacillus cereus (KF270349), Klebsiella pneumoniae (KF270350), Bacillus subtilis (KF270351), Brevibacterium halotolerance (KF270352), Pseudomonas aeruginosa (KF270353), and Stenotrophomonas rhizoposid (KF270354) by ribotyping. All strains were PHA producers so were selected for PHA synthesis using four different carbon sources, i.e., waste frying oil, canola oil, diesel and glucose....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Waste frying oil; Polyhydroxyalkanoates; Fluorescence microscopy; FTIR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400629

Production and characterization of PHA from recombinant E. coli harbouring phaC1 gene of indigenous Pseudomonas sp. LDC-5 using molasses
Provedor de dados: 58
Autores: Saranya,V; Shenbagarathai,R.
Polyhydroxyalkanoates (PHA) are biodegradable and biocompatible green thermoplastics, synthesized by wide variety of bacteria as an intracellular carbon and energy storage intermediate. They are used as an alternative to nonrenewable petroleum derived plastics. The current interest in these biopolyesters is stimulated by the search for cost-effective capitalized production. This paper attempts to achieve maximized production rate from recombinant system using inexpensive substrate. Molasses from agro-industrial waste was used to produce PHA from recombinant E.coli in batch culture. PHA yield in molasses (3.06g/L ± 0.05-75.5%) was higher than that of sucrose (2.5g/L ± 0.05 - 65.1%). Properties of the polymer produced from molasses and sucrose were analyzed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyhydroxyalkanoates; Molasses; Cost effective production.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300032

New PHA products using unrelated carbon sources
Provedor de dados: 58
Autores: Matias,Fernanda; Rodrigues,Maria Filomena de Andrade.
Polyhydroxyalkanoates (PHA) are natural polyesters stored by a wide range of bacteria as carbon source reserve. Due to its chemical characteristics and biodegradability PHA can be used in chemical, medical and pharmaceutical industry for many human purposes. Over the past years, few Burkholderia species have become known for production of PHA. Aside from that, these bacteria seem to be interesting for discovering new PHA compositions which is important to different industrial applications. In this paper, we introduce two new strains which belong either to Burkholderia cepacia complex (Bcc) or genomovar-type, Burkholderia cepacia SA3J and Burkholderia contaminans I29B, both PHA producers from unrelated carbon sources. The classification was based on 16S...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Burkholderia; Burkholderia cepacia complex; Genomovar-type; Mcl-PHA; Polyhydroxyalkanoates; Polyhydroxybutyrate.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400017

Growth kinetics, effect of carbon substrate in biosynthesis of mcl-PHA by Pseudomonas putida Bet001
Provedor de dados: 58
Autores: Gumel,A.M.; Annuar,M.S.M.; Heidelberg,T.
Growth associated biosynthesis of medium chain length poly-3-hydroxyalkanoates (mcl-PHA) in Pseudomonas putida Bet001 isolated from palm oil mill effluent was studied. Models with substrate inhibition terms described well the kinetics of its growth. Selected fatty acids (C8:0 to C18:1) and ammonium were used as carbon and nitrogen sources during growth and PHA biosynthesis, resulting in PHA accumulation of about 50 to 69% (w/w) and PHA yields ranging from 10.12 g L-1 to 15.45 g L-1, respectively. The monomer composition of the PHA ranges from C4 to C14, and was strongly influenced by the type of carbon substrate fed. Interestingly, an odd carbon chain length (C7) monomer was also detected when C18:1 was fed. Polymer showed melting temperature (Tm) of 42.0...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biopolymers; Kinetics; Polyesters; Polyhydroxyalkanoates; Pseudomonas.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200009