Consulta: subjectFacets:"Proliferation"
Registros recuperados: 60
Data/hora: 01/06/2024 10:07:08

Knockdown of E2f1 by RNA interference impairs proliferation of rat cells in vitro
Provedor de dados: 74
Autores: Vasques,Luciana dos Reis; Pujiz,Regiane Simoni; Strauss,Bryan Eric; Krieger,José Eduardo.
E2F1 plays a key role in cell-cycle regulation in mammals, since its transcription factor activity controls genes required for DNA synthesis and apoptosis. E2F1 deregulation is a common feature among different tumor types and can be a major cause of cell proliferation. Thus, blocking E2F1 expression by RNA interference represents a promising therapeutic approach. In this study, the introduction of specific short hairpin RNAs (shRNAs) reduced E2f1 expression by up to 77%, and impaired rat glioma cell proliferation by approximately 70%, as compared to control cells. Furthermore, we investigated the expression of E2f1 target genes, Cyclin A and Cyclin E. Cyclin A was found to be down-regulated, whereas Cyclin E had similar expression to control cells,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNAi; ShRNA; E2F1; Proliferation; Cancer.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100005

Di and tripeptides from marine sources can target adipogenic process and contribute to decrease adipocyte number and functions
Provedor de dados: 5
Autores: Ben Henda, Yesmine; Laamari, Mariem; Lanneluc, Isabelle; Travers, Marie-agnes; Agogue, Helene; Arnaudin, Ingrid; Bridiau, Nicolas; Maugard, Thierry; Piot, Jean-marie; Sannier, Frederic; Bordenave-juchereau, Stephanie.
The effect of 11 marine-derived cryptides was investigated on proliferation, differentiation and maturation of human white pre-adipocytes (HWP). They were all formerly identified as potent Angiotensin-Converting-Enzyme inhibitors.Val-Trp (VW),Val-Tyr (VY), Lys-Tyr (KY), Lys-Trp (KW), Ile-Tyr (IY), Ala-Pro (AP),Val-Ile-Tyr (VIY), Leu-Lys-Pro (LKP), Gly-Pro-Leu (GPL), Ala-Lys-Lys (AKK) and Val-Ala-Pro (VAP) were previously found in fish products and coproducts as well as other marine resources like wakame. Treatment with AP, VAP and AKK greatly affected viability of HWP during the proliferation period while KW and VW treatment reduced the number of viable cells during the differentiation stage. A GPL and IY incubation during the differentiation stage allowed...
Tipo: Text
Palavras-chave: Bioactive peptides; Obesity; Human white pre-adipocytes'; Proliferation; Differentiation; Adipocyte differentiation markers.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00269/38014/36308.pdf

Inventaire des Ulves en Bretagne année 1998 - rapport de synthèse
Provedor de dados: 5
Autores: Merceron, Michel.
The 1998 survey of green tides on the Brittany coast was performed in the same way as that of 1997 in order to allow comparison. 11 includes an inventory ofail the concerned sites, and a precise assessment ofthirteen ofthem, the most loaded. On these ones, nitrogen fluxes were surveyed during the critical period, and the green seaweed stock on the beach and in the first waves was quantified. The climatic conditions were opposite between the two years, very dry in spring and summer 1997, and wet in 1998. The irradiance contrast was inverse, and less pronounced. The nitrate contents and mostly the flows were more important in 1998. They generated nitrogen fluxes about 3.3 times those of 1997. The total number of sites concerned in 1998 is clearly higher than...
Tipo: Text
Palavras-chave: Bretagne; Nitrate; Prolifération; Ulve; Eutrophisation; Brittany; Nitrate; Proliferation; Ulva; Eutrophication.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/rapport-2665.pdf

Marées vertes en Bretagne : état actuel des connaissances
Provedor de dados: 5
Autores: Merceron, Michel.
On tens of sandy beaches of Brittany, a green seaweed (Ulva) blooms during spring and summer. In the sites where the proliferation is the most important, the total biomass amounted to 20 000 t of wet weight in 1997, at the time of maximum. These "green tides" occur every year in nearly the same locations. They have a very harmful impact on local tourism. In many communes, beached and rotting algae are collected and dumped (43 000 m 3 as a whole in 1997). The main development of biomass takes place between the surf zone and the bare sand.The outbreak requires three coexisting conditions : 1) a sandy beach gently sloping, 2) a weak residual current (i.e. once the tidal back and forth movement has been filtered), 3) a nitrogen loading, even moderate (ammonium...
Tipo: Text
Palavras-chave: Brittany; Eutrophication; Proliferation; Ulva; Marine algae; Bretagne; Eutrophisation; Prolifération; Ulves; Algues marines.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/acte-46.pdf

Etude de la prolifération des algues vertes dans le bassin d'Arcachon
Provedor de dados: 5
Autores: Manaud, Francois; Maurer, Daniele; Trut, Gilles; Auby, Isabelle.
Introduction  Massive proliferations of algae and, in particular green macroalgae, have been known all over the continents for several decades. They affect various littoral sites and belong to diverse species (Ulva, Enteromorpha and especially Cladophora). In 1990, on the North Brittany shore, more than 80 000 m3 ulvas have been mechanically removed by affected communities. Up until recently, the Arcachon basin escaped from such phenomenon, even if the diversity and quantity of algae found there had already undergone increasing periods in the past. However, the first algae proliferations only started in the 80's, which scale was large enough to be a nuisance to all activities taking place in the basin.  At first (starting in1982), those proliferations were...
Tipo: Text
Palavras-chave: Basin of Arcachon; Green seaweed; Proliferation; Bassin d'Arcachon; Algue verte; Proliferation.
Ano: 1994
URL: http://archimer.ifremer.fr/doc/1994/rapport-1653.pdf

Inventaire des ulves en Bretagne. Année 1997. Rapport de synthese
Provedor de dados: 5
Autores: Merceron, Michel.
The yearly study of Ulva in Brittany aims at building a time series of its proliferations and corresponding nutrient loadings. The goal is to detect results of wilful loading reductions, and to distinguish them from natural variations. This first actually quantified study is the starting bench-mark of the process. Forty-five concerned sites were listed in 1997. They are nearly the same as in 1988, 1991 and 1994 (previous rough census). The Côtes d'Armor and then the Finistère are the most affected departments. The Morbihan is less and file et Vilaine not at all. Ulva biomass was precisely quantified in about fifteen locations during the growing season, along with nitrogen loadings of local streams (mostly nitrate). The fluxes of nitrogen - the limiting...
Tipo: Text
Palavras-chave: Bretagne; Nitrate; Prolifération; Ulve; Eutrophisation; Brittany; Nitrate; Proliferation; Ulva; Eutrophication.
Ano: 1998
URL: http://archimer.ifremer.fr/doc/1998/rapport-1580.pdf

Antiproliferative Properties of Scandium Exopolysaccharide Complexes on Several Cancer Cell Lines
Provedor de dados: 5
Autores: Muñoz-garcia, Javier; Mazza, Mattia; Alliot, Cyrille; Sinquin, Corinne; Colliec-jouault, Sylvia; Heymann, Dominique; Huclier-markai, Sandrine.
Antimetastatic properties on both murine and human osteosarcoma cell lines (POS-1 and KHOS) have been evidenced using exopolysaccharide (EPS) derivatives, produced by Alteromonas infernus bacterium. These derivatives had no significant effect on the cell cycle neither a pro-apoptotic effect on osteosarcoma cells. Based on this observation, these EPSs could be employed as new drug delivery systems for therapeutic uses. A theranostic approach, i.e., combination of a predictive biomarker with a therapeutic agent, has been developed notably by combining with true pair of theranostic radionuclides, such as scandium 47Sc/44Sc. However, it is crucial to ensure that, once complexation is done, the biological properties of the vector remain intact, allowing the...
Tipo: Text
Palavras-chave: Exopolysaccharides; Scandium; Theranostic; Cancer cell lines; Proliferation.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00686/79826/82630.pdf

Tumorhead distribution to cytoplasmic membrane of neural plate cells is positively regulated by Xenopus p21-activated kinase 1 (X-PAK1)
Provedor de dados: 5
Autores: Wu, Chuan-fen; Delsert, Claude; Faure, Sandrine; Traverso, Edwin E.; Kloc, Malgorzata; Kuang, Jian; Etkin, Laurence D.; Morin, Nathalie.
Tumorhead (TH) regulates neural plate cell proliferation during Xenopus early development, and gain or loss of function prevents neural differentiation. TH shuttles between the nuclear and cytoplasmic/cortical cell compartments in embryonic cells. In this study, we show that subcellular distribution of TH is important for its functions. Targeting TH to the cell cortex/membrane potentiates a TH gain of function phenotype and results in neural plate expansion and inhibition of neuronal differentiation. We have found that TH subcellular localization is regulated, and that its shuttling between the nucleus and the cell cortex/cytoplasm is controlled by the catalytic activity of p21-activated kinase, X-PAK1. The phenotypes of embryos that lack, or have excess,...
Tipo: Text
Palavras-chave: Actin cytoskeleton; Differentiation; Proliferation; Neural plate; P21 activated kinase; Tumorhead.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2996.pdf

Effects of Supplemental Boron on Intestinal Proliferation and Apoptosis in African Ostrich Chicks
Provedor de dados: 77
Autores: Sun,PengPeng; Luo,You; Wu,Xin Tong; Ansari,Abdur Rahman; Wang,Jing; Yang,KeLi; Xiao,Ke; Peng,KeMei.
Boron is an essential trace element which plays an important role in process of metabolism and the function of the tissues. However, the effects of boron on the intestinal cells in African ostrich chicks are poorly reported. Therefore, this study was designed to investigate the role of boron on proliferation and apoptosis of the intestinal cells. A total of 36, ten day-old ostrich chicks were randomly divided into six groups and fed on the same basal diet supplemented with 0, 40, 80, 160, 320 and 640 mg/L boric acid in drinking water for 80 days. Proliferatingcell nuclearantigen (PCNA) wasused to test the proliferation indexof intestine in different group byimmunohistochemicalstaining (IHC). Apoptoticcellsofintestinewere detectedbyDutp-biotin nick end...
Tipo: Journal article
Palavras-chave: Apoptosis; Boron; Intestine; Ostrich chicks; Proliferation.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000300002

Early Proliferation of Bone Marrow Mononuclear Cells on Collagen Membrane, Bone Graft and Tooth Cementum
Provedor de dados: 77
Autores: Milagres,Débora; Rueff-Barroso,Carlos R; Carvalho,Simone N; Carvalho,Laís de; Monte-Alto-Costa,Andréa; Fischer,Ricardo G; Porto,Luís Cristóvão.
The ultimate goal of periodontal therapy is to repair the damaged periodontal supporting tissues, permitting regeneration of the periodontal ligament. However, the cell response, the supportive matrix and the bioactive molecules use have not yet been well established. Bone marrow mononuclear cells were extracted from rat femurs and tibiae and cultured on a cross-linked collagen membrane, bone graft, or molar tooth to compare cell attachment and early proliferation on these materials. Cell attachment was quantified by light microscopy at 24, 48 and 72h, and cell proliferation was observed under a SEM after 72h. After 24h, the number of cells on bone graft was similar to that of the control and more than twice compared to collagen membrane (q=7.473...
Tipo: Journal article
Palavras-chave: Stem cells; Collagen membrane; Bone graft; Cementum; Proliferation.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400045

MICE TESTICULAR DAMAGE ELICITED BY MALATHION
Provedor de dados: 77
Autores: Bustos-Obregón,Eduardo; González-Hormazábal,Patricio.
Organophosphorous pesticides are a healh problem worldwide, mostly for agroworkers, which are around 2600 millions of person in the year 2001. Little is known about male reproductive damage elicited by these chemicals. The acute effects of malathion (single intraperitoneal injection), 1/12 the LD50 to mice were analyzed at 1, 8, 16, 35 and 40 days after injection, in terms of spermatogenic cell proliferation and apoptosis and of Sertoli cell compromise as revealed by immunocytochemical detection of CK-18 (cytokeratin). The micronuclei test was done to assess for genotoxic activity of the agropesticide. The results showed decreased germ cell number followed by compensatory spermatogonial proliferation by 16 days (as shown by thymidine-H3 testicular uptake),...
Tipo: Journal article
Palavras-chave: Spermatogenesis; Organophosphorous agropesticides; Proliferation; Apoptosis.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000200010

Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines
Provedor de dados: 77
Autores: Ili,Carmen G; Brebi,Priscilla; Garcia,Patricia; Leal,Pamela; Lopez,Jaime; Tapia,Oscar; Letelier,Pablo; Weber,Helga; Castillo,Jonathan; Roa,Juan C.
Overexpression of Short and Raji variants of Cellular FLICE-like inhibitory protein (c-FLIP) is capable of inhibiting apoptosis, while the function of the Long isoform depends of c-FLIPL concentration in cells. The aim of this study was to determine the effects of c-FLIPL knockdown in cervical cell lines. SiHa, C-4I and C-33A cervical cancer cell lines were analyzed. c-FLIPL level expression was determined by quantitative real-time PCR and western blotting. c-FLIPL was transiently downregulated by siRNA. The effects of knockdown of c-FLIPL on cell viability, proliferation and apoptosis were assessed by comparing with scrambled siRNA-transfected cells. SiHa and C-4I c-FLIPL knockdown cells showed increased viability compared with scrambled siRNA-transfected...
Tipo: Journal article
Palavras-chave: Apoptosis; Proliferation; Cervical cancer; SiRNA; C-FLIPL.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200036

Malathion Affects Spermatogenic Proliferation in Mouse
Provedor de dados: 77
Autores: Penna-Videau,S; Bustos-Obregón,E; Cermeño-Vivas,J. R; Chirino,D.
The restriction of the mechanisms of cell proliferation in murine seminiferous epithelium, in terms of induction of programmed cell death until recently has not been fully analyzed. The aim of this work was to assess the effect of Malathion (MP) on testicular morphology and function in mouse spermatogenesis. For the experiments, male albino mice of strain NMRI-IVIC, weighing between 30-40 g were used, and divided into control and experimental groups of 5 each. The animals of the experimental groups were injected with a single dose of MP: 241mg/kg weight (1/12 LD 50 ) resuspended in 0.9% saline, intraperitoneally. Animals were sacrificed at 8.3, 16.6 and 33.2 days post-injection (first, second and third spermatogenic cycles). Testicular samples were...
Tipo: Journal article
Palavras-chave: Malathion; Testis; Mouse; Apoptosis; Morphology; P53; Proliferation.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400023

Cytokinins in the in vitro multiplication and analysis of the volatile fraction of Hyptis marrubioides
Provedor de dados: 124
Autores: Rodrigues,Mariane Aparecida; Bertolucci,Suzan Kelly Vilela; Alvarenga,Ivan Caldeira Almeida; Silva,Sâmia Torres; Carvalho,Alexandre Alves de; Pinto,José Eduardo Brasil Pereira.
ABSTRACT The aim of this study was to evaluate different concentrations of two cytokinins in the in vitro propagation of the medicinal plant Hyptis marrubioides Epl. and to analyse the volatile fraction of the compounds. Nodal segments of plants were inoculated onto Murashige and Skoog (MS) solid medium with different concentrations of benzylaminopurine (BAP) or tidiazuron (TDZ) under a 16/8 h light/dark cycle at 25 ± 2 °C. After 45 days growth, the plants were evaluated for the number and length of the shoots and for shoot dry matter. The volatile constituents were analysed by headspace-GC/MS. The greatest number of shoots was obtained with 1.0 mg L-1 TDZ in the MS medium. The greatest values ​​for shoot length and dry matter were obtained with BAP....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth regulators; Proliferation; Terpenes; Medicinal plant; Hyptis smarrubioides.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902019000100090

Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279
Provedor de dados: 1
Autores: Kavand, Somayeh; Agricultural Biotechnology Research Institute of Iran; Kermani, Maryam Jafarkhani; Agricultural Biotechnology Research Institute of Iran; Haghnazari, Ali; Agricultural Biotechnology Research Institute of Iran; Khosravi, Pegah; Agricultural Biotechnology Research Institute of Iran; Azimi, Mohamad Reza; Agricultural Biotechnology Research Institute of Iran.
In Iran, a large number of Rosa species have been exposed to extinction and therefore preservation techniques are necessary to safeguard their future. In the present investigation, the objectives were to optimize the micropropagation and medium-term conservation of one of these wild species, Rosa pulverulenta. At proliferation stage, the maximum number of new leaves (9.6) were produced on the medium containing 4 &amp;micro;M BAP + 0 &amp;micro;M GA3, whereas the maximum number of axillary shoots (4.1) were observed in the medium containing 4 &amp;micro;M BAP + 3 &amp;micro;M GA3. The results for rooting experiments suggested that the highest increase in stem height (49.5 mm) at the acclimatization stage was observed in plantlets treated with 1 &amp;micro;M...
Tipo: Laboratory analysis
Palavras-chave: In vitro conservation; Micropropagation; Proliferation; Recovery; Rooting; Rosa pulverulenta in vitro conservation; Micropropagation; Proliferation; Recovery; Rooting; Rosa pulverulenta.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279

Entry, dispersion and differentiation of microglia in the developing central nervous system
Provedor de dados: 42
Autores: NAVASCUÉS,JULIO; CALVENTE,RUTH; MARÍN-TEVA,JOSÉ L.; CUADROS,MIGUEL A.
Microglial cells within the developing central nervous system (CNS) originate from mesodermic precursors of hematopoietic lineage that enter the nervous parenchyma from the meninges, ventricular space and/or blood stream. Once in the nervous parenchyma, microglial cells increase in number and disperse throughout the CNS; these cells finally differentiate to become fully ramified microglial cells. In this article we review present knowledge on these phases of microglial development and the factors that probably influence them.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microglia; Hematopoietic lineage; Meninges; Migration; Proliferation.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000100013

Neurochemical phenotype and birthdating of specific cell populations in the chick retina
Provedor de dados: 42
Autores: calaza,Karin da Costa; Gardino,Patricia F.
The chick embryo is one of the most traditional models in developing neuroscience and its visual system has been one of the most exhaustively studied. The retina has been used as a model for studying the development of the nervous system. Here, we describe the morphological features that characterize each stage of the retina development and studies of the neurogenesis period of some specific neurochemical subpopulations of retinal cells by using a combination of immunohistochemistry and autoradiography of tritiated-thymidine. It could be concluded that the proliferation period of dopaminergic, GABAergic, cholinoceptive and GABAceptive cells does not follow a common rule of the neurogenesis. In addition, some specific neurochemical cell groups can have a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Developing; Neurogenesis; Neurotransmitter systems; Ontogenesis; Proliferation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000300007

A spleen tyrosine kinase inhibitor attenuates the proliferation and migration of vascular smooth muscle cells
Provedor de dados: 48
Autores: Seo,Hyang&amp;#8209;Hee; Kim,Sang Woo; Lee,Chang Youn; Lim,Kyu Hee; Lee,Jiyun; Choi,Eunhyun; Lim,Soyeon; Lee,Seahyoung; Hwang,Ki&amp;#8209;Chul.
Abstract  Background  Pathologic vascular smooth muscle cell (VSMC) proliferation and migration after vascular injury promotes the development of occlusive vascular disease. Therefore, an effective chemical agent to suppress aberrant proliferation and migration of VSMCs can be a potential therapeutic modality for occlusive vascular disease such as atherosclerosis and restenosis. To find an anti-proliferative chemical agent for VSMCs, we screened an in-house small molecule library, and the selected small molecule was further validated for its anti-proliferative effect on VSMCs using multiple approaches, such as cell proliferation assays, wound healing assays, transwell migration assays, and ex vivo aortic ring assay.  Results  Among 43 initially screened...
Tipo: Journal article
Palavras-chave: Syk kinase inhibitor; BAY61-3606 VSMC; Proliferation; Migration.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100403

Knockdown of ubiquitin-specific peptidase 39 inhibited the growth of osteosarcoma cells and induced apoptosis in vitro
Provedor de dados: 48
Autores: Gan,Zhihua; Han,Kun; Lin,Shuchen; Hu,Haiyan; Shen,Zan; Min,Daliu.
Abstract  Background  Ubiquitin specific peptidase 39 (USP39), an essential factor in the assembly of the mature spliceosome complex, has an aberrant expression in several cancer. However, its function and the corresponding mechanism on human osteosarcoma has not been fully explored yet.  Methods  The mRNA and DNA copies of USP39 were increased in osteosarcoma cancer tissues compared with the one in human normal tissues according to datasets from the publicly available Oncomine database. A further western blot analysis also demonstrated an aberrant endogenous expression of USP39 in three different osteosarcoma cells. Then lentivirus-mediated short hairpin RNA (shRNA) was designed to silence USP39 in human osteosarcoma cell line U2OS, which is used to test...
Tipo: Journal article
Palavras-chave: Cell cycle; Migration; Osteosarcoma; Proliferation; USP39.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100210

The possible role of Myosin light chain in myoblast proliferation
Provedor de dados: 48
Autores: ZHANG,SU-ZHEN; XU,YONG; XIE,HUI-QI; LI,XIU-QUN; WEI,YU-QUAN; YANG,ZHI-MING.
Skeletal muscles have the potential to regenerate by activation of quiescent satellite cells, however, the molecular signature that governs satellite cells during muscle regeneration is not well defined. Myosin light chains (Myls) are sarcomere-related proteins as traditional regulator of muscle contraction. In this report, we studied the possible role of Myl in the proliferation of skeletal muscle-derived myoblasts. Compared to diaphragm-derived myoblasts, the extraocular muscle-derived myoblasts with lower levels of Myl proliferated faster, maintained a longer proliferation phase, and formed more final myotubes. It was found that blockading Myl with anti-Myl antibody or knockdown of Myll by siRNA targeted against Myll could enhance the myoblast...
Tipo: Journal article
Palavras-chave: Myosin light chain; Proliferation; Myoblast; Extraocular muscle.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100013

Simultaneous silencing of VEGF and KSP by siRNA cocktail inhibits proliferation and induces apoptosis of hepatocellular carcinoma Hep3B cells
Provedor de dados: 48
Autores: Doan,Chung Chinh; Le,Long Thanh; Hoang,Son Nghia; Do,Si Minh; Van Le,Dong.
BACKGROUND: Vascular endothelial growth factor (VEGF) is involved in the growth of new blood vessels that feed tumors and kinesin spindle protein (KSP) plays a critical role in mitosis involving in cell proliferation. Simultaneous silencing of VEGF and KSP, an attractive and viable approach in cancer, leads on restricting cancer progression. The purpose of this study is to examine the therapeutic potential of dual gene targeted siRNA cocktail on human hepatocellular carcinoma Hep3B cells. RESULTS: The predesigned siRNAs could inhibit VEGF and KSP at mRNA level. siRNA cocktail showed a further downregulation on KSP mRNA and protein levels compared to KSP-siRNA or VEGF-siRNA, but not on VEGF expression. It also exhibited greater suppression on cell...
Tipo: Journal article
Palavras-chave: Vascular endothelial cell growth factor (VEGF); Kinesin spindle protein (KSP); SiRNA cocktail; Proliferation; Apoptosis; Hepatocellular carcinoma.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100064

TMPYP4 exerted antitumor effects in human cervical cancer cells through activation of p38 mitogen-activated protein kinase
Provedor de dados: 48
Autores: Cheng,Ming&amp;#8209;Jun; Cao,Yun&amp;#8209;Gui.
Abstract  Background  The aim of the present study was to investigate the potential effects of the 5,10,15,20&amp;#8209;tetrakis (1&amp;#8209;meth&amp;#8209; ylpyridinium&amp;#8209;4&amp;#8209;yl) porphyrin (TMPyP4) on the proliferation and apoptosis of human cervical cancer cells and the underlying mechanisms by which TMPyP4 exerted its actions.  Results  After human cervical cancer cells were treated with different doses of TMPyP4, cell viability was determined by 3&amp;#8209;(4,5&amp;#8209;dimethyl&amp;#8209;2&amp;#8209;thiazolyl)&amp;#8209;2,5&amp;#8209;diphenyl&amp;#8209;2&amp;#8209;H&amp;#8209;tetrazolium bromide (MTT) method, the apoptosis was observed by flow cytometry (FCM), and the expression of p38 mitogen&amp;#8209;activated protein kinase...
Tipo: Journal article
Palavras-chave: TMPyP4; P38 MAPK; Human cervical cancer cells; Proliferation; Apoptosis.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100217

MicroRNA-98 inhibits the cell proliferation of human hypertrophic scar fibroblasts via targeting Col1A1
Provedor de dados: 48
Autores: Bi,Sheng; Chai,Linlin; Yuan,Xi; Cao,Chuan; Li,Shirong.
Abstract  Background  Hypertrophic scarring (HS) is a severe disease, and results from unusual wound healing. Col1A1 could promote the hypertrophic scar formation, and the expression of Col1A1 in HS tissue was markedly higher than that in the normal. In present study, we aimed to identify miRNAs as post-transcriptional regulators of Col1A1 in HS.  Methods  MicroRNA-98 was selected as the key miRNA comprised in HS. The mRNA levels of miR-98 in HS tissues and the matched normal skin tissues were determined by qRT-PCR. MTT and flow cytometry were used to determine the influence of miR-98 on cell proliferation and apoptosis of HSFBs, respectively. Col1A1 was found to be the target gene of miR-98 using luciferase reporter assay. Luciferase assay was performed...
Tipo: Journal article
Palavras-chave: MiR-98; Col1A1; Apoptosis; Proliferation; HSFBs.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100215

Protective effects of lycopene on oxidative stress, proliferation and autophagy in iron supplementation rats
Provedor de dados: 48
Autores: Liu,Chongbin; Wang,Rui; Zhang,Bo; Hu,Chen; Zhang,Hong.
Lycopene is common in diet and known for its antioxidant activities. However, the impact of lycopene on iron metabolism is poorly investigated. In this study, we hypothesize that lycopene can prevent iron-mediated oxidative stress, proliferation and autophagy in liver and use a rat model of nutritional iron supplementation to confirm its intervention in these defence mechanisms. We found that iron supplementation induced cell proliferation predominantly in non parenchymal cells compared with hepatocytes, but not apoptosis. In addition, iron was accumulated within the hepatic lysosomes where it triggered autophagy as evidenced by the formation of autophagic vesicles detected by LC3-B staining. Iron supplementation also induced morphologic alterations of the...
Tipo: Journal article
Palavras-chave: Autophagy; Iron supplementation; Lycopene; Oxidative stress; Proliferation.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200011

BRD4 inhibition suppresses cell growth, migration and invasion of salivary adenoid cystic carcinoma
Provedor de dados: 48
Autores: Wang,Limei; Wu,Xiuyin; Wang,Ruolin; Yang,Chengzhe; Li,Zhi; Wang,Cunwei; Zhang,Fenghe; Yang,Pishan.
Abstract  Background: Bromodomain-containing protein 4 (BRD4) inhibition is a new therapeutic strategy for many malignancies. In this study, we aimed to explore the effect of BRD4 inhibition by JQ1 on in vitro cell growth, migration and invasion of salivary adenoid cystic carcinoma (SACC).  Methods: The human normal epithelial cells and SACC cells (ACC-LM and ACC-83) were treated with JQ1 at concentrations of 0, 0.1, 0.5 or 1 &amp;#956;M. Cell Counting Kit-8 (CCK-8) assay was performed to evaluate cell proliferation. Cell apoptosis and cell cycle distribution was evaluated by Flow cytometry. Immunofluorescence staining was used to examine the expression of BRD4 in SACC cells. The quantitative real-time polymerase chain reaction (qRT-PCR) assay and western...
Tipo: Journal article
Palavras-chave: Salivary adenoid cystic carcinoma; BRD4 inhibition; Proliferation; Migration; Epithelial-mesenchymal transition.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100213

Galectin-8 promotes migration and proliferation and prevents apoptosis in U87 glioblastoma cells
Provedor de dados: 48
Autores: Metz,Claudia; Döger,Remziye; Riquelme,Elizabeth; Cortés,Priscilla; Holmes,Christopher; Shaughnessy,Ronan; Oyanadel,Claudia; Grabowski,Catalina; González,Alfonso; Soza,Andrea.
BACKGROUND: Glioblastoma is one of the most aggressive cancers of the brain. Malignant traits of glioblastoma cells include elevated migration, proliferation and survival capabilities. Galectins are unconventionally secreted glycan-binding proteins that modulate processes of cell adhesion, migration, proliferation and apoptosis by interacting with beta-galactosides of cell surface glycoproteins and the extracellular matrix. Galectin-8 is one of the galectins highly expressed in glioblastoma cells. It has a unique selectivity for terminally sialylated glycans recently found enhanced in these highly malignant cells. A previous study in glioblastoma cell lines reported that Gal-8 coating a plastic surface stimulates two-dimensional motility. Because in other...
Tipo: Journal article
Palavras-chave: Galectin-8; Glioblastoma; Cancer; Cell cycle; Apoptosis; Proliferation; Migration.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100033

Fetal Cerebrospinal Fluid Promotes Proliferation and Neural Differentiation of Stromal Mesenchymal Stem Cells Derived from Bone Marrow
Provedor de dados: 52
Autores: Shokohi,Rozmehr; Nabiuni,Mohammad; Moghaddam,Parisa; Irian,Saeed; Miyan,Jaleel A.
ABSTRACT Embryonic cerebrospinal fluid (E-CSF) contains many neurotrophic and growth factors, acts as a growth medium for cortical progenitors, and can modulate proliferation and differentiation of neural stem cells. Mesenchymal stem cells (MSCs) are multipotential stem cells that can differentiate into several types of mesenchymal cells as well as nonmesenchymal cells, such as neural cells. In the present study, the effect of E-CSF on proliferation and neural differentiation of bone marrow mesenchymal stem cells (BM-MSCs) was investigated to test whether E-CSF is capable of driving these cells down the neuronal line. To verify the multipotential characteristics of BM-MSCs, the cells were analyzed for their osteogenic and adipogenic potential. Expression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebrospinal fluid; Neural differentiation; Proliferation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100441

Effects of plant lectins on in vitro fibroblast proliferation
Provedor de dados: 52
Autores: Sell,Ana Maria; Costa,Celso Paulino da.
Lectins are carbohydrate-binding proteins that have been isolated from various sources and presented a wide spectrum of biological activities. The effects of four lectins, namely, Phaseolus vulgaris phytohemagglutinin, PHA, wheat germ agglutinin, WGA, Artocarpus integrifolia seed lectins, jacalin and artocarpin, on in vitro fibroblasts proliferation were investigated. The lectins did not influence the initial cell adhesion to the plate. PHA and WGA at 10-20 µg/mL concentrations significantly decreased fibroblasts proliferation. At these concentrations, they caused morphological alterations on cells and over 80 µg/mL, promoted cell death. Neither jacalin nor artocarpin significantly affected cell proliferation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lectins; Fibroblasts; Proliferation; MTT.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300006

Efficient Regeneration of Eucalyptus urophylla x Eucalyptus grandis from Stem Segment
Provedor de dados: 52
Autores: Ouyang,Lejun; Huang,Zhenchi; Zhao,Longyan; Sha,Yuee; Zeng,Fuhua; Lu,Xiangyang.
The aim of the present study was to establish an efficient regeneration system for the hybrid E. urophylla x E. grandis by means of organogenesis. Stem segments from seedlings were used as explants and cultured in a modified Murashige and Skoog medium (MS), supplemented with 13.2 µM N-phenyl-N'-[6-(2-chlorobenzothiazol)-yl] urea (PBU) and 0.285 µM indole-3-acetic acid (IAA). PBU was a useful growth regulator. After cultivating for 5 d, 96% explants formed callus. After 30 d, the calli obtained were transferred to MS medium containing different combinations of 6-benzyladenine (BA) and naphthalene acetic acid (NAA). Compared with other growth regulator combinations, PBU stimulated more vigorous calli and restrained browning. In addition, a large percentage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro regeneration; Bud induction; Proliferation; Substituted urea.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300001

In vitro micropropagation of Stevia rebaudiana Bertoni in Malaysia
Provedor de dados: 52
Autores: Razak,Ummi Nur Ain Abdul; Ong,Chong Boon; Yu,Tiew Sing; Lau,Li Kiaw.
Stevia rebaudiana Bertoni is a medicinal plants and commercially use as non-caloric sweetener for diabetic patient. In the present study, a protocol was developed for in vitro micropropagation using 6-benzylamino purine (BAP) and Kinetin (Kn) for the formation of multiple shoot proliferation and Indole-3-acetic acid (IAA), Indole-3-butyric acid (IBA) and 1-Naphthaleneacetic acid (NAA) for the induction of roots. Maximum shoot formation (7.82 ± 0.7 shoots per explants) was observed on a Murashige and Skoog (MS) medium supplemented with 0.5 mg L-1 BAP and 0.25 mg L-1 Kn. The maximum number of roots (30.12 ± 2.1 roots per explants) was obtained on a MS medium containing 1.0 mg L-1 IBA. The well rooted plantlets were successfully weaned and acclimatized in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MS medium; Non-caloric sweetener; Proliferation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100004

The miR-17-92 cluster regulates FOG-2 expression and inhibits proliferation of mouse embryonic cardiomyocytes
Provedor de dados: 56
Autores: Xiang,Rui; Lei,Han; Chen,Mianzhi; Li,Qinwei; Sun,Huan; Ai,Jianzhong; Chen,Tielin; Wang,Honglian; Fang,Yin; Zhou,Qin.
MicroRNAs (miRNAs) have gradually been recognized as regulators of embryonic development; however, relatively few miRNAs have been identified that regulate cardiac development. A series of recent papers have established an essential role for the miRNA-17-92 (miR-17-92) cluster of miRNAs in the development of the heart. Previous research has shown that the Friend of Gata-2 (FOG-2) is critical for cardiac development. To investigate the possibility that the miR-17-92 cluster regulates FOG-2 expression and inhibits proliferation in mouse embryonic cardiomyocytes we initially used bioinformatics to analyze 3’ untranslated regions (3’UTR) of FOG-2 to predict the potential of miR-17-92 to target it. We used luciferase assays to demonstrate that miR-17-5p and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNAs; MiR-17-92; FOG-2; Proliferation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200007

Interleukin-27 augments the inhibitory effects of sorafenib on bladder cancer cells
Provedor de dados: 56
Autores: Cao,J.Y.; Yin,H.S.; Li,H.S.; Yu,X.Q.; Han,X.
Both sorafenib and interleukin-27 (IL-27) are antineoplastic drugs. This study aimed to investigate the synergistic effect of these two drugs on bladder cancer cells. HTB-9 and T24 cells were stimulated with IL-27 (50 ng/mL), sorafenib (2 μM) or the synergistic action of these two drugs. The cells without treatment acted as control. Cell proliferation, apoptosis and invasion were measured by bromodeoxyuridine assay, flow cytometry and modified Boyden chamber, respectively. Simultaneously, both modified Boyden chamber and scratch assay were used to assess cell migration. Finally, the phosphorylation levels of key kinases in the Akt/mechanistic target of rapamycin (mTOR)/mitogen-activated protein kinase (MAPK) pathway, and expression levels of matrix...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sorafenib; Interleukin 27; Proliferation; Apoptosis; Invasion; Akt/mTOR/MAPK.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800610

In vitro proliferation and differentiation of hepatic oval cells and their potential capacity for intrahepatic transplantation
Provedor de dados: 56
Autores: Li,Z.; Chen,J.; Li,L.; Ran,J.H.; Liu,J.; Gao,T.X.; Guo,B.Y.; Li,X.H.; Liu,Z.H.; Liu,G.J.; Gao,Y.C.; Zhang,X.L.
Hepatic oval cells (HOCs) are recognized as facultative liver progenitor cells that play a role in liver regeneration after acute liver injury. Here, we investigated the in vitro proliferation and differentiation characteristics of HOCs in order to explore their potential capacity for intrahepatic transplantation. Clusters or scattered HOCs were detected in the portal area and interlobular bile duct in the liver of rats subjected to the modified 2-acetylaminofluorene and partial hepatectomy method. Isolated HOCs were positive for c-kit and CD90 staining (99.8% and 88.8%, respectively), and negative for CD34 staining (3.6%) as shown by immunostaining and flow cytometric analysis. In addition, HOCs could be differentiated into hepatocytes and bile duct...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatic oval cells; Proliferation; Differentiation; Liver transplantation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800681

A DNA enzyme targeting Egr-1 inhibits rat vascular smooth muscle cell proliferation by down-regulation of cyclin D1 and TGF-β1
Provedor de dados: 56
Autores: Wu,Y.; Han,W.; Liu,G.-N.
We have demonstrated that a synthetic DNA enzyme targeting early growth response factor-1 (Egr-1) can inhibit neointimal hyperplasia following vascular injury. However, the detailed mechanism of this inhibition is not known. Thus, the objective of the present study was to further investigate potential inhibitory mechanisms. Catalytic DNA (ED5) and scrambled control DNA enzyme (ED5SCR) were synthesized and transfected into primary cultures of rat vascular smooth muscle cells (VSMCs). VSMC proliferation and DNA synthesis were analyzed by the MTT method and BrdU staining, respectively. Egr-1, TGF-β1, p53, p21, Bax, and cyclin D1 expression was detected by RT-PCR and Western blot. Apoptosis and cell cycle assays were performed by FACS. Green fluorescence could...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA enzyme; Egr-1; VSMC; Proliferation; Apoptosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100004

MicroRNA-455 suppresses the oncogenic function of HDAC2 in human colorectal cancer
Provedor de dados: 56
Autores: Mao,Q.D.; Zhang,W.; Zhao,K.; Cao,B.; Yuan,H.; Wei,L.Z.; Song,M.Q.; Liu,X.S.
Colorectal cancer (CRC) is the fourth leading cause of cancer-induced mortality. Histone deacetylase 2 (HDAC2) is involved in prognosis and therapy of CRC. This study aimed to explore novel therapeutic targets for CRC. The alteration of HDAC2 expression in CRC tissues was estimated by qRT-PCR. After lentivirus transfection, HDAC2 knockdown was confirmed by western blot analysis. The effect of HDAC2 knockdown on cell proliferation was then assessed by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Screened by TargetScan, microRNA (miR)-455 was predicted to bind to 3′UTR of HDAC2 and the prediction was verified by luciferase assay. Finally, cells were transfected, respectively, with miR-455 mimics or miR-455 negative control...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colorectal cancer; Histone deacetylase 2; MiR-455; Proliferation; Apoptosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600608

Proliferation and osteogenic activity of fibroblasts induced with fibronectin
Provedor de dados: 56
Autores: Zhang,W.-H.; Li,X.-L.; Guo,Y.; Zhang,Y.
The aim of this study was to determine the proliferation and osteogenic activity of fibroblasts induced with fibronectin and their possible dose-dependent relationship. The fibroblasts obtained by tissue explants adherent method were induced with fibronectin at different concentrations of 0, 10, 20, 40, 60, and 80 μg/mL for 14 days. The 3H-thymidine and 3H-proline incorporation test was used to evaluate the synthesis of DNA and collagen by fibroblasts, respectively. The mineralized nodules and osteocalcin secretion, as vital osteogenic indicators, were detected with tetracycline labeling and 125I-labeled competitive immunoassay, respectively. Fibronectin significantly increased the synthesis of DNA and collagen by fibroblasts, especially at the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibroblast; Fibronectin; Proliferation; Osteogenesis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000604

Adipose tissue-derived stem cells promote pancreatic cancer cell proliferation and invasion
Provedor de dados: 56
Autores: Ji,S.Q.; Cao,J.; Zhang,Q.Y.; Li,Y.Y.; Yan,Y.Q.; Yu,F.X.
To explore the effects of adipose tissue-derived stem cells (ADSCs) on the proliferation and invasion of pancreatic cancer cells in vitroand the possible mechanism involved, ADSCs were cocultured with pancreatic cancer cells, and a cell counting kit (CCK-8) was used to detect the proliferation of pancreatic cancer cells. ELISA was used to determine the concentration of stromal cell-derived factor-1 (SDF-1) in the supernatants. RT-PCR was performed to detect the expression of the chemokine receptor CXCR4 in pancreatic cancer cells and ADSCs. An in vitro invasion assay was used to measure invasion of pancreatic cancer cells. SDF-1 was detected in the supernatants of ADSCs, but not in pancreatic cancer cells. Higher CXCR4 mRNA levels were detected in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adipose tissue-derived stem cells; Pancreatic cancer; Proliferation; Invasion; SDF-1.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900758

Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU 145 human prostate cancer cells
Provedor de dados: 56
Autores: Vucic,V.; Isenovic,E.R.; Adzic,M.; Ruzdijic,S.; Radojcic,M.B.
Gamma-irradiation (gamma-IR) is extensively used in the treatment of hormone-resistant prostate carcinoma. The objective of the present study was to investigate the effects of 60Co gamma-IR on the growth, cell cycle arrest and cell death of the human prostate cancer cell line DU 145. The viability of DU 145 cells was measured by the Trypan blue exclusion assay and the 3(4,5-dimethylthiazol-2-yl)-2,5,diphenyltetrazolium bromide test. Bromodeoxyuridine incorporation was used for the determination of cell proliferation. Cell cycle arrest and cell death were analyzed by flow cytometry. Superoxide dismutase (SOD), specifically CuZnSOD and MnSOD protein expression, after 10 Gy gamma-IR, was determined by Western immunoblotting analysis. gamma-IR treatment had a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gamma-irradiation; Prostate cancer; Proliferation; Cell death; Cell cycle arrest; Superoxide dismutase.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200009

NOP14 inhibits melanoma proliferation and metastasis by regulating Wnt/β-catenin signaling pathway
Provedor de dados: 56
Autores: Li,Jingrong; Fang,Ruihua; Wang,Jianqin; Deng,Liehua.
Malignant melanoma is an aggressive skin cancer with a high mortality rate. Nucleolar protein 14 (NOP14) has been implicated in cancer development. However, the role of NOP14 in malignant melanoma progression remains largely unclear. In this study, we observed that malignant melanoma tissue showed NOP14 down-regulation compared to melanocytic nevi tissues. Moreover, we observed that NOP14 expression was significantly associated with melanoma tumor thickness and lymph node metastasis. NOP14 overexpression in melanoma cells suppressed proliferation, caused G1 phase arrest, promoted apoptosis, and inhibited melanoma cell migration and invasion. Further investigations revealed that NOP14 overexpression reduced the expression levels of Wnt3a, β-catenin, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malignant melanoma; Metastasis; NOP14; Proliferation; Wnt/β-catenin pathway.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100604

Targeting PPM1D by lentivirus-mediated RNA interference inhibits the tumorigenicity of bladder cancer cells
Provedor de dados: 56
Autores: Wang,W.; Zhu,H.; Zhang,H.; Zhang,L.; Ding,Q.; Jiang,H.
Protein phosphatase magnesium/manganese-dependent 1D (PPM1D) is a p53-induced phosphatase that functions as a negative regulator of stress response pathways and has oncogenic properties. However, the functional role ofPPM1D in bladder cancer (BC) remains largely unknown. In the present study, lentivirus vectors carrying small hairpin RNA (shRNA) targeting PPM1D were used to explore the effects ofPPM1D knockdown on BC cell proliferation and tumorigenesis. shRNA-mediated knockdown of PPM1D significantly inhibited cell growth and colony forming ability in the BC cell lines 5637 and T24. Flow cytometric analysis showed that PPM1D silencing increased the proportion of cells in the G0/G1 phase. Downregulation of PPM1Dalso inhibited 5637 cell tumorigenicity in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protein phosphatase magnesium/manganese-dependent 1D; Bladder cancer; Gene silencing; RNA interference; Proliferation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201044

Nuclear calcium signaling: a cell within a cell
Provedor de dados: 56
Autores: Rodrigues,M.A.; Gomes,D.A.; Nathanson,M.H.; Leite,M.F.
Calcium (Ca2+) is a versatile second messenger that regulates a wide range of cellular functions. Although it is not established how a single second messenger coordinates diverse effects within a cell, there is increasing evidence that the spatial patterns of Ca2+ signals may determine their specificity. Ca2+ signaling patterns can vary in different regions of the cell and Ca2+ signals in nuclear and cytoplasmic compartments have been reported to occur independently. No general paradigm has been established yet to explain whether, how, or when Ca2+ signals are initiated within the nucleus or their function. Here we highlight that receptor tyrosine kinases rapidly translocate to the nucleus. Ca2+ signals that are induced by growth factors result from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium; Proliferation; Receptor tyrosine kinases; Nucleus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100004

Effect of curcumin on the proliferation and apoptosis of hepatic stellate cells
Provedor de dados: 56
Autores: Shu,J.C.; He,Y.J.; Lv,X.; Zhao,J.R.; Zhao,J.; Shen,Y.; Ye,G.R.; Wang,L.X.
This study was designed to investigate the effect of curcumin (diferuloylmethane) on the proliferation and apoptosis of hepatic stellate cells (HSC). The cell line HSC-T6 (1.25 x 10(5) cells/mL) was incubated with curcumin and HSC proliferation was detected by a methyl thiazolyl tetrazolium colorimetric assay. HSC apoptosis was detected by flow cytometry, transmission electron microscope and agarose gel electrophoresis. HSC proliferation was significantly inhibited in a concentration-dependent manner (10.6 to 63.5%) after incubation with 20-100 μM curcumin, compared with a control group. At 20, 40, and 60 μM, after 24 h of incubation, curcumin was associated with a significant increase in the number of HSC in the G2/M phase, and a significant decrease in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curcumin; Hepatic stellate cells; Proliferation; Apoptosis; Hepatic fibrosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200009

Retinoic acid and cAMP inhibit rat hepatocellular carcinoma cell proliferation and enhance cell differentiation
Provedor de dados: 56
Autores: Ionta,M.; Rosa,M.C.; Almeida,R.B.; Freitas,V.M.; Rezende-Teixeira,P.; Machado-Santelli,G.M.
Hepatocellular carcinoma (HCC) is the third highest cause of cancer death worldwide. In general, the disease is diagnosed at an advanced stage when potentially curative therapies are no longer feasible. For this reason, it is very important to develop new therapeutic approaches. Retinoic acid (RA) is a natural derivative of vitamin A that regulates important biological processes including cell proliferation and differentiation. In vitro studies have shown that RA is effective in inhibiting growth of HCC cells; however, responsiveness to treatment varies among different HCC cell lines. The objective of the present study was to determine if the combined use of RA (0.1 µM) and cAMP (1 mM), an important second messenger, improves the responsiveness of HCC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell adhesion; CAMP; Differentiation; Proliferation; Hepatocarcinoma; Retinoic acid.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800006

SULF2 promotes tumorigenesis and inhibits apoptosis of cervical cancer cells through the ERK/AKT signaling pathway
Provedor de dados: 56
Autores: Jiang,Tao; Chen,Zhao-Hui; Chen,Zhe; Tan,Dan.
The objective of this study was to explore the role of the SULF2-mediated ERK/AKT signaling pathway in cervical cancer. SULF2 expression was detected in tumor tissues and tumor-adjacent normal tissues from cervical cancer patients. HeLa cells were divided into six groups: control group, NC group, SULF2 siRNA group, SULF2 group, SULF2 + LY294002 group, and SULF2 + U0125 group. In each group, HeLa cells received the corresponding treatment, followed by measurement of the cellular biological characteristics and expression of the ERK/AKT signaling pathway. We also confirmed the effect of SULF2 in vivo using a xenograft model in nude mice. SULF2 was upregulated in cervical cancer tissues, which was specifically associated with the clinical stage, histological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SULF2; Cervical cancer; ERK/AKT; Proliferation; Apoptosis.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200608

Whole-body vibration decreases the proliferativeb response of TCD4+ cells in elderly individuals with knee osteoarthritis
Provedor de dados: 56
Autores: Tossige-Gomes,R.; Avelar,N.C.P.; Simão,A.P.; Neves,C.D.C.; Brito-Melo,G.E.A.; Coimbra,C.C.; Rocha-Vieira,E.; Lacerda,A.C.R.
The aim of this study was to investigate the effect of adding whole-body vibration (WBV; frequency = 35 to 40 Hz; amplitude = 4 mm) to squat training on the T-cell proliferative response of elderly patients with osteoarthritis (OA) of the knee. This study was a randomized controlled trial in which the selected variables were assessed before and after 12 weeks of training. Twenty-six subjects (72 ± 5 years of age) were divided into three groups: 1) squat training with WBV (WBV, N = 8); 2) squat training without WBV (N = 10), and 3) a control group (N = 8). Women who were ≥60 years of age and had been diagnosed with OA in at least one knee were eligible. The intervention consisted of 12 uninterrupted weeks of squatting exercise training performed 3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vibratory platform; Lymphocyte; Proliferation; Elderly; Osteoarthritis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200022

Expression of CHPF modulates cell proliferation and invasion in lung cancer
Provedor de dados: 56
Autores: Cao,Chengsong; Liu,Yong; Wang,Qun; Zhao,Jing; Shi,Ming; Zheng,Junnian.
Lung cancer is the most common malignancy worldwide and is characterized by rapid progression, aggressive behavior, frequent recurrence, and poor prognosis. The TCGA database indicates that chondroitin polymerizing factor (CHPF) is overexpressed in human lung cancer tissues compared with normal tissues and this overexpression corresponds to shorter overall survival in lung cancer patients. In this study, to investigate the function of CHPF in lung cancer, lentiviral vectors expressing CHPF shRNA were stably transduced into A549 and H1299 cells. Compared to shCtrl cells, CHPF knockdown cells had significantly reduced proliferation. Furthermore, the silencing of CHPF in A549 and H1299 cells resulted in apoptotic induction, which led to decreased colony...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CHPF; Lung cancer; Proliferation; Invasion; TCGA; Affymetrix Gene Chip.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500610

Leukocytosis, muscle damage and increased lymphocyte proliferative response after an adventure sprint race
Provedor de dados: 56
Autores: Tossige-Gomes,R.; Ottone,V.O.; Oliveira,P.N.; Viana,D.J.S.; Araújo,T.L.; Gripp,F.J.; Rocha-Vieira,E.
The effect of an adventure sprint race (ASR) on T-cell proliferation, leukocyte count and muscle damage was evaluated. Seven young male runners completed an ASR in the region of Serra do Espinhaço, Brazil. The race induced a strong leukocytosis (6.22±2.04×103 cells/mm3 beforevs 14.81±3.53×103 cells/mm3after the race), marked by a significant increase of neutrophils and monocytes (P&lt;0.05), but not total lymphocytes, CD3+CD4+ or CD3+CD8+ cells. However, the T-cell proliferative response to mitogenic stimulation was increased (P=0.025) after the race, which contradicted our hypothesis that ASR, as a high-demand competition, would inhibit T-cell proliferation. A positive correlation (P=0.03, r=0.79) was observed between the proliferative response of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Strenuous exercise; Lymphocyte; Proliferation; Aspartate amino transferase; Immunosuppression.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600492

Serotonin regulates osteoblast proliferation and function in vitro
Provedor de dados: 56
Autores: Dai,S.Q.; Yu,L.P.; Shi,X.; Wu,H.; Shao,P.; Yin,G.Y.; Wei,Y.Z.
The monoamine serotonin (5-hydroxytryptamine, 5-HT), a well-known neurotransmitter, also has important functions outside the central nervous system. The objective of this study was to investigate the role of 5-HT in the proliferation, differentiation, and function of osteoblasts in vitro. We treated rat primary calvarial osteoblasts with various concentrations of 5-HT (1 nM to 10 µM) and assessed the rate of osteoblast proliferation, expression levels of osteoblast-specific proteins and genes, and the ability to form mineralized nodules. Next, we detected which 5-HT receptor subtypes were expressed in rat osteoblasts at different stages of osteoblast differentiation. We found that 5-HT could inhibit osteoblast proliferation, differentiation, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Serotonin; Osteoblast; Proliferation; Differentiation; 5-HT receptors; 5-HT.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900759

Low-intensity pulsed ultrasound promotes proliferation and migration of HaCaT keratinocytes through the PI3K/AKT and JNK pathways
Provedor de dados: 56
Autores: Leng,Xiaoyan; Shang,Jing; Gao,Danhui; Wu,Jiang.
Although the effects of low-intensity pulsed ultrasound (LIPUS) on diverse cell types have been fully studied, the functional role of LIPUS in keratinocytes remains poorly understood. This study aimed to investigate the effects of LIPUS on proliferation and migration of HaCaT cells as well as the regulatory mechanisms associated with signaling pathways. Human HaCaT cells were exposed or not to LIPUS, and cell proliferation and migration were measured by BrdU incorporation assay and Transwell assay, respectively. Expression of proteins associated with proliferation and migration was evaluated by western blot analysis. Expression of key kinases in the PI3K/AKT and JNK pathways was also evaluated by western blot analysis. Effects of LIPUS on the PI3K/AKT and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Low-intensity pulsed ultrasound; HaCaT cells; Proliferation; Migration; PI3K/AKT/JNK.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200603

Aging reduces the primary humoral response and the in vitro cytokine production in mice
Provedor de dados: 56
Autores: Simioni,P.U.; Costa,E.H.; Tamashiro,W.M.S.C.
Aging is accompanied by a decrease in several physiological functions that make older individuals less responsive to environmental challenges. In the present study, we analyzed the immune response of female BALB/c mice (N = 6) of different ages (from 2 to 96 weeks) and identified significant age-related alterations. Immunization with hapten-protein (trinitrophenyl-bovine serum albumin) conjugates resulted in lower antibody levels in the primary and secondary responses of old mice (72 weeks old). Moreover, young mice (2, 16, and 32 weeks old) maintained specific antibodies in their sera for longer periods after primary immunization than did old mice. However, a secondary challenge efficiently induced memory in old mice, as shown by the increased antibody...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Cytokines; Antibody; Proliferation; T cell response; T cell subsets.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800012

HtrA1 suppresses the growth of pancreatic cancer cells by modulating Notch-1 expression
Provedor de dados: 56
Autores: Cheng,Hao; Zhu,Hao; Cao,Meng; Lu,Chenglin; Bao,Shanhua; Pan,Yiming.
Pancreatic cancer is well known to be the most deadly malignancy with the worst survival rate of all cancers. High temperature requirement factor A1 (HtrA1) plays an important role in cancer cell proliferation, migration, apoptosis, and differentiation. This study aimed to explore the function of HtrA1 in pancreatic cancer cell growth and its underlying mechanism. We found that the expression of HtrA1 was lower in pancreatic cancer tissue compared to the adjacent normal tissue. Consistently, HtrA1 levels were also decreased in two human pancreatic cancer cell lines, PANC-1 and BXPC-3. Moreover, enforced expression of HtrA1 inhibited cell viability and colony formation of PANC-1 and BXPC-3 cells. Overexpression of HtrA1 promoted apoptosis and suppressed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pancreatic cancer; Proliferation; HtrA1; Notch-1; Molecular target.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100606

Tetramethylpyrazine potentiates arsenic trioxide activity against HL-60 cell lines
Provedor de dados: 56
Autores: Wu,Yuni; Xu,Youhua; Shen,Yali; Wang,Cuicui; Guo,Gaili; Hu,Tiantian.
The objective of this study was to evaluate the effects of tetramethylpyrazine (TMP) in combination with arsenic trioxide (As2O3) on the proliferation and differentiation of HL-60 cells. The HL-60 cells were treated with 300 µg/mL TMP, 0.5 µM As2O3, and 300 µg/mL TMP combined with 0.5 µM As2O3, respectively. The proliferative inhibition rates were determined with MTT. Differentiation was detected by the nitroblue tetrazolium (NBT) reduction test, Wright’s staining and the distribution of CD11b and CD14. Flow cytometry was used to analyze cell cycle distribution. RT-PCR and Western blot assays were employed to detect the expressions of c-myc, p27, CDK2, and cyclin E1. Combination treatment had synergistic effects on the proliferative inhibition rates. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tetramethylpyrazine; Acute promyelocytic leukemia; Arsenic trioxide; HL-60 cell; Proliferation; Differentiation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300002

Silencing of augmenter of liver regeneration inhibited cell proliferation and triggered apoptosis in U266 human multiple myeloma cells
Provedor de dados: 56
Autores: Zeng,H.Q.; Luo,Y.; Lou,S.F.; Liu,Q.; Zhang,L.; Deng,J.C.
Augmenter of liver regeneration (ALR) is a thermostable cytokine that was originally identified to promote the growth of hepatocytes. This study was conducted to explore the expression and function of ALR in multiple myeloma (MM), a common hematologic malignancy. Real-time PCR and western blot analysis were performed to detect the expression of ALR in U266 human MM cells and healthy peripheral blood mononuclear cells (PBMCs). U266 MM cells were exposed to 20 or 40 μg/mL of recombinant ALR and tested for cell proliferation. Small interfering RNA-mediated silencing of ALR was done to investigate the role of ALR in cell proliferation, apoptosis, and cytokine production. Compared to PBMCs, U266 MM cells exhibited significantly higher levels of ALR at both the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Augmenter of liver regeneration; Hematologic cancer; Proliferation; Small interfering RNA.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001000611

High expression of the circadian gene mPer2 diminishes the radiosensitivity of NIH 3T3 cells
Provedor de dados: 56
Autores: Chang,L.; Liu,Y.Y.; Zhu,B.; Li,Y.; Hua,H.; Wang,Y.H.; Zhang,J.; Jiang,Z.; Wang,Z.R.
Period2 is a core circadian gene, which not only maintains the circadian rhythm of cells but also regulates some organic functions. We investigated the effects of mPeriod2 (mPer2) expression on radiosensitivity in normal mouse cells exposed to 60Co-γ-rays. NIH 3T3 cells were treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) to induce endogenous mPer2 expression or transfected with pcDNA3.1(+)-mPer2 and irradiated with 60Co-γ-rays, and then analyzed by several methods such as flow cytometry, colony formation assay, RT-PCR, and immunohistochemistry. Flow cytometry and colony formation assay revealed that irradiated NIH 3T3 cells expressing high levels of mPer2 showed a lower death rate (TPA: 24 h 4.3% vs 12 h 6.8% and control 9.4%; transfection:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Circadian; MPer2; Radiation; Cell death; Proliferation; DNA repair.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000003

Polydatin inhibits cell proliferation, invasion and migration, and induces cell apoptosis in hepatocellular carcinoma
Provedor de dados: 56
Autores: Jiao,Yang; Wu,Yan; Du,Dong.
Polydatin, a small molecule from Polygonum cuspidatum, has many biological functions, particularly anti-cancer effects. However, the anti-cancer effects of polydatin in hepatocellular carcinoma (HCC) have not been examined yet. In the present study, MTT assay, BrdU assay, transwell invasion assay, and wound healing assay were performed to determine cell proliferation, invasion and migration. Flow cytometry and TUNEL assay were used to measure cell apoptosis. Quantitative real-time PCR and western blotting assays were used to determine mRNA and protein expression levels. Xenograft experiment was performed to determine the in vivo anti-tumor effect of polydatin. Immunostaining was performed to analyze the expression of caspase-3 and Ki-67. Our results showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polydatin; Hepatocellular carcinoma; Proliferation; Apoptosis; Wnt/beta-catenin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400608

Effect of aging and oral tolerance on dendritic cell function
Provedor de dados: 56
Autores: Simioni,P.U.; Fernandes,L.G.R.; Gabriel,D.L.; Tamashiro,W.M.S.C.
Oral tolerance can be induced in some mouse strains by gavage or spontaneous ingestion of dietary antigens. In the present study, we determined the influence of aging and oral tolerance on the secretion of co-stimulatory molecules by dendritic cells (DC), and on the ability of DC to induce proliferation and cytokine secretion by naive T cells from BALB/c and OVA transgenic (DO11.10) mice. We observed that oral tolerance could be induced in BALB/c mice (N = 5 in each group) of all ages (8, 20, 40, 60, and 80 weeks old), although a decline in specific antibody levels was observed in the sera of both tolerized and immunized mice with advancing age (40 to 80 weeks old). DC obtained from young, adult and middle-aged (8, 20, and 40 weeks old) tolerized mice were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; Oral tolerance; Dendritic cell; Antibody; Proliferation; Cytokine secretion.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100010

Disintegrins: integrin selective ligands which activate integrin-coupled signaling and modulate leukocyte functions
Provedor de dados: 56
Autores: Barja-Fidalgo,C.; Coelho,A.L.J.; Saldanha-Gama,R.; Helal-Neto,E.; Mariano-Oliveira,A.; Freitas,M.S. de.
Extracellular matrix proteins and cell adhesion receptors (integrins) play essential roles in the regulation of cell adhesion and migration. Interactions of integrins with the extracellular matrix proteins lead to phosphorylation of several intracellular proteins such as focal adhesion kinase, activating different signaling pathways responsible for the regulation of a variety of cell functions, including cytoskeleton mobilization. Once leukocytes are guided to sites of infection, inflammation, or antigen presentation, integrins can participate in the initiation, maintenance, or termination of the immune and inflammatory responses. The modulation of neutrophil activation through integrin-mediated pathways is important in the homeostatic control of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Integrin; Disintegrin; Leukocytes; Activation; Proliferation; Signal transduction.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000008

Circular RNA hsa_circ_0000523 regulates the proliferation and apoptosis of colorectal cancer cells as miRNA sponge
Provedor de dados: 56
Autores: Jin,Y.; Yu,L.L.; Zhang,B.; Liu,C.F.; Chen,Y.
Among the novel class of endogenous long non-coding RNAs, circular RNA (circRNA) is known as a key regulator in the development and progression of different cancers. Its function and mechanism in the tumorigenesis of colorectal cancer, however, has not been well studied. This study thus aimed to investigate potential regulation of colorectal cancer by circRNAs and the corresponding regulatory mechanism. We demonstrated that the expression of circRNA hsa_circ_0000523 (also known as circ_006229) was down-regulated in different colorectal cancer cell lines. It was also found that interference of hsa_circ_0000523 induced proliferation and suppressed apoptosis of colorectal cancer cells, the proliferation rate of which was reduced by the overexpression of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Circular RNA hsa_circ_0000523; Colorectal cancer; Proliferation; Sponge; Interference.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200611

microRNA-3129 promotes cell proliferation in gastric cancer cell line SGC7901 via positive regulation of pRb
Provedor de dados: 56
Autores: Yang,Shaofeng; Sheng,Nan; Pan,Lili; Cao,Jing; Liu,Jiao; Ma,Ran.
Several microRNAs (miRNAs) have been reported as oncogenes or tumor suppressors in many cancers, including gastric cancer (GC). However, the role and molecular mechanism of miR-3129 in GC is largely unknown. We aimed to explore the function and the underlying molecular mechanism of miR-3129 in GC. Cancer tissues and corresponding adjacent tissues were collected from 50 patients with GC, and the expression of miR-3129 was detected by RT-qPCR. The expression of miR-3129 and pRb in human GC cell line SCG7091 was altered by transient transfection. Thereafter, MTT and flow cytometry assays were used to analyze cell viability and cell cycle. The expression of cyclin E, CDK2, CDK2 inhibitors (p16 and 21), and pRb were detected by RT-qPCR and western blot. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNA-3129; Gastric cancer; PRb; Proliferation; Cell cycle.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600612

PRODUCTION OF OVOGONIA BY GERMINATIVE EPITHELIUM OF THE OVARY AT SOME MAMMALIA AND BIRDS
Provedor de dados: 65
Autores: Sincai,Mariana; Marcu,Adrian.
The studies were effected on ovaries from sows, cows, bitches, dous and hens. Our investigations revealed the generation of cellular rows of germinative epithelium into the cortex not only during the embrionary period but also in the adult ovary. The histological investigations revealed an intense proliferative process of the germinative epithelium of adult ovary which generated groups of ovogonia under the lamina basalis. From ovogonia developed oocytes and then ovarian follicles. The cause for the proliferation of ovary germinative epithelium could be: the multiple folliculary dehiscences, the premature degeneration of ovarian follicles and formation of the corpus luteum, phenomena that reduce continually the number of embrionary ovogonia.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovary; Epithelium; Mammalian; Hens; Proliferation; Ovogonia; Oocytes; Ovarian follicles.
Ano: 1993
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781993000300018