Consulta: subjectFacets:"Prostaglandin"
Registros recuperados: 6
Data/hora: 01/06/2024 07:21:47

Acumulación de ácido araquidónico en fosfolípidos y triglicéridos y producción de prostaglandinas con relación a estrés térmico en semilla de ostión Crassostrea gigas
Provedor de dados: 5
Autores: Duran Encinas, Yazmin.
Crassostrea gigas is a marine invertebrate that inhabits the intertidal zone and therefore, is commonly exposed to multiple environmental stressors, including temperature. In recent years mass mortality events have been reported in Mexico in C. gigas associated with the increase in temperature during the summer. Organisms under thermal stress present an increase in prostaglandins (PG) production by cyclooxygenases (COX), an enzymatic route using arachidonic acid (ARA) present in the phospholipids (FL) of the cell membrane as substrate, but PG can also increase non-enzymatically by reactive oxygen species (ROS) that are produced during increased metabolism. It has been reported that some organisms exposed to stress accumulate a higher concentration of ARA...
Tipo: Text
Palavras-chave: Arachidonic acid; Stress; Phospholipid; Isotope; Mollusk; Temperature; Triglyceride; Prostaglandin.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00620/73193/72387.pdf

Determination of Prostaglandins in the Colostrum of Dairy Cattle by Thermospray High-Performance Liquid Chromatography-Mass Spectrometry.
Provedor de dados: 19
Autores: FUKUSHIMA, Michihiro; NAKANO, Masuo; ENDO, Tsuyoshi; NEGISHI, Takashi; 福島, 道広.
A thermospray high-performance liquid chromatography-mass spectrometry (TSP HPLC-MS) method is described for the analysis of prostaglandins (PGs) E1 and E2, which have several physiological actions at the trace level. HPLC separation was carried out on a reversed-phase column by using 50mM ammonium acetate (pH 4.5)-acetonitrile (7:3, v/v) as the mobile phase at a flow rate of 1.0ml/min. In MS detection, the vaporizer temperature was set at 220°C and the ion-source block temperature at 264°C. The mass spectra obtained from PGs typically exhibited the [M+NH4]+ ion (5 to 30% relative intensity) and several fragment ions due to sequential losses of water from both the [M+H]+ and [M+NH4]+ ions. PGE1 (46.9ppb) and PGE2 (1.9ppb) in the colostrum of dairy cattle...
Palavras-chave: Thermospray liquid chromatography-mass spectrometry; Prostaglandin; Colostrum; Dairy cattle.
Ano: 1993
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4042

INHIBITION OF MAYARO VIRUS REPLICATION BY PROSTAGLANDIN A1 IN Aedes albopictus CELLS
Provedor de dados: 59
Autores: Barbosa,Joel Antonio; Rebello,Moacyr Alcoforado.
Prostaglandin A1 (PGA1) inhibits Mayaro virus replication in Aedes albopictus cells at nontoxic doses to uninfected cells. At 10 µg/ml, PGA1 decreases virus production by 90%. The presence of PGA1 during virus adsorption, with no treatment after infection, reduces virus yield by 41%. Antiviral activity is observed even when treatment starts at one or two hours post-infection. However, in cells pre-treated with PGA1 during 24 hours, virus replication is not impaired. Thus, events ocurring during initial stages of infection and after virus adsorption and penetration must be the target of PGA1 action. SDS-PAGE analysis of 35S-methionine labelled proteins shows that PGA1 inhibits the synthesis of viral proteins and induces the synthesis of polypeptides with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mayaro virus; Prostaglandin; Aedes albopictus cells; Heat-shock proteins; Actinomycin D.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300013

Role of non-nitric oxide non-prostaglandin endothelium-derived relaxing factor(s) in bradykinin vasodilation
Provedor de dados: 56
Autores: Resende,A.C.; Ballejo,G.; Salgado,M.C.O.
The most conspicuous effect of bradykinin following its administration into the systemic circulation is a transient hypotension due to vasodilation. In the present study most of the available evidence regarding the mechanisms involved in bradykinin-induced arterial vasodilation is reviewed. It has become firmly established that in most species vasodilation in response to bradykinin is mediated by the release of endothelial relaxing factors following the activation of B2-receptors. Although in some cases the action of bradykinin is entirely mediated by the endothelial release of nitric oxide (NO) and/or prostacyclin (PGI2), a large amount of evidence has been accumulated during the last 10 years indicating that a non-NO/PGI2 factor accounts for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostaglandin; Bradykinin; Nitric oxide; Cytochrome P450; Potassium channels; Endothelium-dependent vasodilation.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900017

Effect of prostaglandin A1 in the induction of stress proteins in Aedes albopictus cells
Provedor de dados: 56
Autores: Barbosa,J.A.; Rebello,M.A.
Prostaglandins are natural fatty acid derivatives with diverse physiological effects, including immune function and the control of cell growth. While the action of prostaglandins in the induction of stress proteins in vertebrate cells is well documented, their functions in invertebrate cells have been poorly investigated. The purpose of the present study was to investigate the effect of prostaglandin A1 (PGA1; 0.25, 1.25 and 12.5 µg/ml) on protein synthesis during the growth of Aedes albopictus cells. We found that PGA1 stimulates the synthesis of several polypeptides with molecular masses of 87, 80, 70, 57, 29, 27 and 23 kDa in Aedes albopictus cells. When the proteins induced by PGA1 and those induced by heat treatment were compared by polyacrylamide gel...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Prostaglandin; Stress proteins; Aedes albopictus cells.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400004

The L-arginine/nitric oxide/cyclic-GMP pathway apparently mediates the peripheral antihyperalgesic action of fentanyl in rats
Provedor de dados: 56
Autores: Maegawa,F.A.B.; Tonussi,C.R.
There are only a few studies on the molecular mechanisms underlying the peripheral antihyperalgesic effect of opioids. The aim of this study was to investigate the molecular bases of the peripheral antihyperalgesic effect of fentanyl in a model of prostaglandin-induced chemical hyperalgesia. Prostaglandin E2 (1.4 nmol) injected into one hind paw of male Wistar rats (200-250 g, N = 6 in each experimental or control group) pretreated with indomethacin (2.5 mg/kg) potentiated the nocifensive response to formalin (1%) injection made 60 min later. Drugs applied locally 30 min after prostaglandin E2 induced the following effects: fentanyl (0.1-1.0 nmol) caused a dose-dependent reversal of the hyperalgesic state, naloxone (2 nmol) co-injected with fentanyl (1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Opioid; Guanylate cyclase; Prostaglandin; PDE5; UK-114; 542-27.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200012