Consulta: subjectFacets:"Protein interactions"
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Data/hora: 06/06/2024 01:18:46

A calcineurin inhibitory protein overexpressed in Down's syndrome interacts with the product of a ubiquitously expressed transcript
Provedor de dados: 56
Autores: Silveira,H.C.S.; Sommer,C.A.; Soares-Costa,A.; Henrique-Silva,F.
The Down's syndrome candidate region 1 (DSCR1) protein, encoded by a gene located in the human chromosome 21, interacts with calcineurin and is overexpressed in Down's syndrome patients. As an approach to clarifying a putative function for this protein, in the present study we used the yeast two-hybrid system to identify DSCR1 partners. The two-hybrid system is a method that allows the identification of protein-protein interactions through reconstitution of the activity of the yeast GAL 4 transcriptional activator. The gene DSCR1 fused to the GAL 4 binding domain (BD) was used to screen a human fetal brain cDNA library cloned in fusion with the GAL 4 activation domain (AD). Three positive clones were found and sequence analysis revealed that all the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Down's syndrome; Down's syndrome candidate region 1; Calcineurin; Two-hybrid system; Ubiquitously expressed transcript; Protein interactions.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600002