Consulta: subjectFacets:"Protein synthesis"
Registros recuperados: 13
Data/hora: 13/06/2024 13:58:29

Тканевые особенности белкового синтеза у двустворчатого моллюска Anadaria inaeguivalvis (Bruguiere) в условиях нормы и при дефиците пищи
Provedor de dados: 13
Autores: Щербань, С. А.
Results of the comparative investidation of protein synthesis levels in gills, hepatopancreas, legs and somatic tissues of different age–size groups of mussels Anadara inaeguivalvis Br. (normal condition) and under nutrition stress are present. It was shown the protein synthesis process have the expressed tissue pecilitiry, differences of synthesis levels were observed for somatic tissues only. Biosynthesis process in anadara tissues were charactirised by different directions under the nutrition stress. Thus, the tendency of biosynthesis levels increase or decrease in gills was absent; the synthesis level in legs tissues in 1,3 times decreased.
Tipo: Book Section
Palavras-chave: Anadara inaeguivalvis; Protein synthesis; Tissue features; RNK; Index RNK/DNA; Deficit food.
Ano: 2010
URL: http://repository.ibss.org.ua/dspace/handle/99011/1884

The anti-fecundity effect of 5-azacytidine (5-AzaC) on Schistosoma mansoni is linked to dis-regulated transcription, translation and stem cell activities
Provedor de dados: 5
Autores: Geyer, Kathrin K.; Munshi, Sabrina E.; Vickers, Martin; Squance, Michael; Wilkinson, Toby J.; Berrar, Daniel; Chaparro, Cristian; Swain, Martin T.; Hoffmann, Karl F.
Uncontrolled host immunological reactions directed against tissue-trapped eggs precipitate a potentially lethal, pathological cascade responsible for schistosomiasis. Blocking schistosome egg production, therefore, presents a strategy for simultaneously reducing immunopathology as well as limiting disease transmission in endemic or emerging areas. We recently demonstrated that the ribonucleoside analogue 5-azacytidine (5-AzaC) inhibited Schistosoma mansoni oviposition, egg maturation and ovarian development. While these anti-fecundity effects were associated with a loss of DNA methylation, other molecular processes affected by 5-AzaC were not examined at the time. By comparing the transcriptomes of 5-AzaC-treated females to controls, we provide evidence...
Tipo: Text
Palavras-chave: Schistosoma mansoni; Epigenetics; 5-Azacytidine; Fecundity; RNA-Seq; Protein synthesis; Stem cells.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00615/72703/71747.pdf

Effects of cottonseed meal supplemented with lysine and enzyme (Hydroenzyme XP) on egg quality and performance of laying hens
Provedor de dados: 96
Autores: Saki,Ali Asghar; Pournia,Khashayar; Tabatabaie,Mohamad Mehdi; Zamani,Pouya; Haghighat,Mojtaba; Salary,Jalal.
This study was conducted to evaluate the effect of cottonseed meal supplemented with lysine and enzyme on laying hens performance and egg quality. Eighty Hy-Line W-36 white Leghorns were allotted for 12 weeks in a 2 × 2 factorial experiment in a completely randomized design, including four treatments and five replications with four birds in each. Treatments included: basal diet + 1% lysine + 0% enzyme; basal diet + 1% lysine + 0.025% enzyme; basal diet + 2% lysine + 0% enzyme; and basal diet + 2% lysine + 0.025% enzyme. Protein content in the magnum tissue was not significantly affected by different levels of lysine and enzyme, although magnum Protein:RNA ratio increased with 2% of lysine as compared with 1%. Moreover, jejunum DNA's concentration was not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cottonseed meal; DNA; Performance; Protein synthesis; RNA.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000013

Endogenous ADP-ribosylation of eukaryotic elongation factor 2 and its 32 kDa tryptic fragment
Provedor de dados: 47
Autores: Ergen,,Kivanç; Bektas¸,Muhammet; Gökçe,Sina; Nurten,Rüstem.
Eukaryotic elongation factor 2 (eEF-2) can undergo ADP-ribosylation in the absence of diphtheria toxin. The binding of free ADP-ribose and endogenous transferase-dependent ADP-ribosylation were distinct reactions for eEF-2, as indicated by different findings. Incubation of eEF-2 tryptic fragment 32/33 kDa (32F) with NAD was ADP-ribosylated and gave rise to the covalent binding of ADP-ribose to eEF-2. 32F was revealed to be at the C-terminal by Edman degradation sequence analysis. In our study, the elution of 32F from SDS-PAGE was ADP-ribosylated both in the presence and absence of diphtheria toxin. These results suggest that endogenous ADP-ribosylation of 32F might be related to protein synthesis. This modification appears to be important for the cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eukaryotic elongation factor 2; Endogenous ADP-ribosylation; Protein synthesis; Trypsin digestion; Diphtheria toxin.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100007

Protein synthesis in eukaryotes: The growing biological relevance of cap-independent translation initiation
Provedor de dados: 48
Autores: LÓPEZ-LASTRA,MARCELO; RIVAS,ANDREA; BARRÍA,MARÍA INÉS.
Ribosome recruitment to eukaryotic mRNAs is generally thought to occur by a scanning mechanism, whereby the 40S ribosomal subunit binds in the vicinity of the 5'cap structure of the mRNA and scans until an AUG codon is encountered in an appropriate sequence context. Study of the picornaviruses allowed the characterization of an alternative mechanism of translation initiation. Picornaviruses can initiate translation via an internal ribosome entry segment (IRES), an RNA structure that directly recruits the 40S ribosomal subunits in a cap and 5' end independent fashion. Since its discovery, the notion of IRESs has extended to a number of different virus families and cellular RNAs. This review summarizes features of both cap-dependent and IRES-dependent...
Tipo: Journal article
Palavras-chave: Protein synthesis; Translation initiation; Internal ribosome entry segment.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200003

Local regulation of the axonal phenotype, a case of merotrophism
Provedor de dados: 48
Autores: COURT,FELIPE A; ÁLVAREZ,JAIME.
In this essay, we show that several anatomical features of the axon, namely, microtubular content, caliber and extension of sprouts, correlate on a local basis with the particular condition of the glial cell, i.e., the anatomy of axons is dynamic, although it is seen usually in its `normal' state. The occurrence of ribosomes and messenger RNAs in the axon suggests that axoplasmic proteins are most likely synthesized locally, at variance with the accepted notion that they are supplied by the cell body. We propose that the supporting cell (oligodendrocyte or Schwann cell) regulates the axonal phenotype by fine-tuning the ongoing axonal protein synthesis.
Tipo: Journal article
Palavras-chave: Axon; Protein synthesis; Nerve regeneration; Schwann cell; Wld s.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000400009

Slow axoplasmic transport under scrutiny
Provedor de dados: 48
Autores: Court,Felipe A; Álvarez,Jaime.
The origin of axoplasmic proteins is central for the biology of axons. For over fifty years axons have been considered unable to synthesize proteins and that cell bodies supply them with proteins by a slow transport mechanism. To allow for prolonged transport times, proteins were assumed to be stable, i.e., not degraded in axons. These are now textbook notions that configure the slow transport model (STM). The aim of this article is to cast doubts on the validity of STM, as a step toward gaining more understanding about the supply of axoplasmic proteins. First, the stability of axonal proteins claimed by STM has been disproved by experimental evidence. Moreover, the evidence for protein synthesis in axons indicates that the repertoire is extensive and the...
Tipo: Journal article
Palavras-chave: Protein synthesis; Radioactive wave; Maintenance of axons; Schwann cell; Glia; Transgenic mouse.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400001

Inhibition of Mayaro virus replication by prostaglandin A1 and B2 in Vero cells
Provedor de dados: 56
Autores: Ishimaru,D.; Marcicano,F.G.P.; Rebello,M.A.
The effect of prostaglandins (PGA1 and PGB2) on the replication of Mayaro virus was studied in Vero cells. PGA1 and PGB2 antiviral activity was found to be dose-dependent. However, while 10 µg/ml PGB2 inhibited virus yield by 60%, at the same dose PGA1 suppressed virus replication by more than 90%. SDS-PAGE analysis of [35S]-methionine-labelled proteins showed that PGA1 did not alter cellular protein synthesis. In infected cells, PGA1 slightly inhibited the synthesis of protein C, while drastically inhibiting the synthesis of glycoproteins E1 and E2.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Prostaglandins; Mayaro virus; Vero cells; Protein synthesis.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900003

Thyroid hormone regulates protein expression in C6 glioma cells
Provedor de dados: 56
Autores: Trentin,A.G.; Alvarez-Silva,M.
Thyroid hormone (T3) is essential to normal brain development. Previously, we have shown that T3 induces cerebellar astrocyte proliferation. This effect is accompanied by alteration in glial fibrillary acidic protein (GFAP) and fibronectin organization. In the present study, we report that the C6 glioma cell line, which expresses GFAP and is classified as an undifferentiated astrocytic cell type, is a target for T3 action. The C6 monolayers were treated with 50 nM T3 for 3 days, after which the cells were maintained for 2 days without medium changes. In C6 cells, T3 induced the expression of proteins of 107, 73 and 62 kDa. The hormone also up-regulated protein bands of 100 (+50%), 37 (+50%) and 25.5 kDa (+50%) and down-regulated proteins of 94 (-100%),...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Thyroid hormone; Glioma; Protein synthesis; GFAP; Vimentin; Differentiation.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000008

Short-term creatine supplementation changes protein metabolism signaling in hindlimb suspension
Provedor de dados: 56
Autores: Marzuca-Nassr,G.N.; Fortes,M.A.S.; Guimarães-Ferreira,L.; Murata,G.M.; Vitzel,K.F.; Vasconcelos,D.A.A.; Bassit,R.A.; Curi,R.
The effect of a short-term creatine supplementation on hindlimb suspension (HS)-induced muscle atrophy was investigated. Creatine monohydrate (5 g/kg b.w. per day) or placebo, divided in 2 daily doses, was given by oral gavage for 5 days. Rats were maintained in HS with dietary supplementation concomitantly for 5 days. Body weight, soleus and EDL muscle masses, and cross-sectional areas (CSA) of the muscle fibers were measured. Signaling pathways associated with skeletal muscle mass regulation (FST, MSTN, FAK, IGF-1, MGF, Akt, mTOR, atrogin-1, and MuRF1 expressions, and Akt, S6, GSK3B, and 4EBP1 proteins) were evaluated in the muscles. Soleus muscle exhibited more atrophy than the EDL muscle due to HS. Creatine supplementation attenuated the decrease of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Creatine supplementation; Hindlimb suspension; Muscle disuse atrophy; Protein synthesis; Protein degradation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000606

Myostatin promotes distinct responses on protein metabolism of skeletal and cardiac muscle fibers of rodents
Provedor de dados: 56
Autores: Manfredi,L.H.; Paula-Gomes,S.; Zanon,N.M.; Kettelhut,I.C.
Myostatin is a novel negative regulator of skeletal muscle mass. Myostatin expression is also found in heart in a much less extent, but it can be upregulated in pathological conditions, such as heart failure. Myostatin may be involved in inhibiting protein synthesis and/or increasing protein degradation in skeletal and cardiac muscles. Herein, we used cell cultures and isolated muscles from rats to determine protein degradation and synthesis. Muscles incubated with myostatin exhibited an increase in proteolysis with an increase of Atrogin-1, MuRF1 and LC3 genes. Extensor digitorum longus muscles and C2C12 myotubes exhibited a reduction in protein turnover. Cardiomyocytes showed an increase in proteolysis by activating autophagy and the ubiquitin proteasome...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myostatin; Skeletal muscle; Cardiomyocytes; Protein degradation; Protein synthesis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200604

Dengue virus-induced regulation of the host cell translational machinery
Provedor de dados: 56
Autores: Villas-Bôas,C.S.A.; Conceição,T.M.; Ramírez,J.; Santoro,A.B.M.; Da Poian,A.T.; Montero-Lomelí,M.
Dengue virus (DV)-induced changes in the host cell protein synthesis machinery are not well understood. We investigated the transcriptional changes related to initiation of protein synthesis. The human hepatoma cell line, HepG2, was infected with DV serotype 2 for 1 h at a multiplicity of infection of one. RNA was extracted after 6, 24 and 48 h. Microarray results showed that 36.5% of the translation factors related to initiation of protein synthesis had significant differential expression (Z-score ≥ ±2.0). Confirmation was obtained by quantitative real-time reverse transcription-PCR. Of the genes involved in the activation of mRNA for cap-dependent translation (eIF4 factors), eIF4A, eIF4G1 and eIF4B were up-regulated while the negative regulator of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dengue virus; Flavivirus; Protein synthesis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100004

Protein synthesis regulation by leucine
Provedor de dados: 60
Autores: Vianna,Daiana; Teodoro,Gabriela Fullin Resende; Torres-Leal,Francisco Leonardo; Tirapegui,Julio.
In vivo and in vitro studies have demonstrated that high protein diets affect both protein synthesis and regulation of several cellular processes. The role of amino acids as substrate for protein synthesis has been established in the literature. However, the mechanism by which these amino acids modulate transcription and regulate the mRNA translation via mTOR-dependent signaling pathway has yet to be fully determined. It has been verified that mTOR is a protein responsible for activating a cascade of biochemical intracellular events which result in the activation of the protein translation process. Of the aminoacids, leucine is the most effective in stimulating protein synthesis and reducing proteolysis. Therefore, it promotes a positive nitrogen balance,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leucine; Protein synthesis; Transcription Factors; MTOR; Translation Protein.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000100004