Consulta: subjectFacets:"Pulsed field gel electrophoresis"
Registros recuperados: 6
Data/hora: 11/06/2024 09:19:01

BABESIA CABALLI : ANALYSIS OF CHROMOSOMES SEPARATED BY PULSE FIELD GEL ELECTROPHORESIS
Provedor de dados: 19
Autores: Ikadai, Hiromi; Kudo, Noboru; Oyamada, Takashi; Igarashi, Ikuo; 五十嵐, 郁男.
Palavras-chave: Babesia caballi; Pulsed field gel electrophoresis; Chromosome; Rhoptry gene.
Ano: 2004
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/642

Genetic Similarities of Escherichia Coli Isolated from Different Substrates of the Broiler Production Chain
Provedor de dados: 89
Autores: Teles,PFS; Boiago,MM; Frigo,A; Rampazzo,L; Araújo,DN; Kich,JD; Rebelatto,R; Furian,TQ; Stefani,LM.
ABSTRACT Brazil is the largest exporter of chicken meat and poultry farming is one of the most important productive segments, despite major losses due to the bacterium Escherichia coli, which is also a zoonotic microorganism. The objetive of this study was to isolate E. coli and to evaluate its transmissibility potential from the field to chicken meat using the Pulsed Field Gel Electrophoresis (PFGE) technique. Environmental samples (poultry litter, soil and water) were collected from broiler farms located in the South of Brazil where the majority of the Brazilian poultry production occurs. In addition, chicken meat (gizzard, heart, drumette and tulip) samples were collected from local supermarkets. As results, 47.36% of the samples were positives for E....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Environment; Epidemiology; Microbiology; Public health; Pulsed field gel electrophoresis.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000100308

Distribution of serotypes and evaluation of antimicrobial susceptibility among human and bovine Streptococcus agalactiae strains isolated in Brazil between 1980 and 2006
Provedor de dados: 55
Autores: Pinto,Tatiana Castro Abreu; Costa,Natália Silva; Souza,Aline Rosa Vianna; Silva,Ligia Guedes da; Corrêa,Ana Beatriz de Almeida; Fernandes,Flavio Gimenis; Oliveira,Ivi Cristina Menezes; Mattos,Marcos Corrêa de; Rosado,Alexandre Soares; Benchetrit,Leslie Claude.
Streptococcus agalactiae is a common agent of clinical and subclinical bovine mastitis and an important cause of human infections, mainly among pregnant women, neonates and nonpregnant adults with underlying diseases. The present study describes the genetic and phenotypic diversity among 392 S. agalactiae human and bovine strains isolated between 1980 and 2006 in Brazil. The most prevalent serotypes were Ia, II, III and V and all the strains were susceptible to penicillin, vancomycin and levofloxacin. Resistance to clindamycin, chloramphenicol, erythromycin, rifampicin and tetracycline was observed. Among the erythromycin resistant strains, mefA/E, ermA and, mainly, ermB gene were detected, and a shift of prevalence from the macrolide resistance phenotype...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus agalactiae; Pulsed field gel electrophoresis; Epidemiology; Serotyping; Resistance profile.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200003

Staphylococcus aureus in tonsils of patients with recurrent tonsillitis: prevalence, susceptibility profile, and genotypic characterization
Provedor de dados: 55
Autores: Cavalcanti,Veraluce Paolini; Camargo,Leandro Azevedo de; Moura,Fabiano Santana; Melo Fernandes,Edson Júnior de; Lamaro-Cardoso,Juliana; Braga,Carla Afonso da Silva Bitencourt; André,Maria Cláudia Porfirio.
ABSTRACT  Introduction: Bacterial tonsillitis is an upper respiratory tract infection that occurs primarily in children and adolescents. Staphylococcus aureus is one of the most frequent pathogens in the etiology of tonsillitis and its relevance is due to its antimicrobial resistance and persistence in the internal tissues of the tonsils. Tonsillectomy is indicated in cases of recurrent tonsillitis after several failures of antibiotic therapy.  Material and methods: In this study we evaluated 123 surgically removed tonsils from patients who had history of recurrent tonsillitis. The tonsils were submitted to microbiological analysis for detection of S. aureus. The isolates were identified by PCR for femA gene. Antimicrobial susceptibility of the isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tonsillitis; Tonsillectomy; Staphylococcus aureus; Pulsed field gel electrophoresis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000100008

Genetic profiling of Klebsiella pneumoniae: comparison of pulsed field gel electrophoresis and random amplified polymorphic DNA
Provedor de dados: 58
Autores: Ashayeri-Panah,Mitra; Eftekhar,Fereshteh; Ghamsari,Maryam Mobarak; Parvin,Mahmood; Feizabadi,Mohammad Mehdi.
In this study, the discriminatory power of pulsed field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) methods for subtyping of 54 clinical isolates of Klebsiella pneumoniae were compared. All isolates were typeable by RAPD, while 3.6% of them were not typeable by PFGE. The repeatability of both typing methods were 100% with satisfying reproducibility (≥ 95%). Although the discriminatory power of PFGE was greater than RAPD, both methods showed sufficient discriminatory power (DI &gt; 0.95) which reflects the heterogeneity among the K. pneumoniae isolates. An optimized RAPD protocol is less technically demanding and time consuming that makes it a reliable typing method and competitive with PFGE.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella pneumoniae; Pulsed field gel electrophoresis; Random amplified polymorphic DNA; Genotyping.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300024

Molecular characterization of Listeria monocytogenes isolated from animal products in a city of Northern Brazil
Provedor de dados: 65
Autores: Monteiro,Lilyan Rosmery Luizaga de; Mesquita,Albenones José de; André,Maria Cláudia Dantas Porfirio Borges; Cardoso,Juliana Lamaro.
Listeria monocytogenes, a foodborne pathogen causes listeriosis, a fatal disease in about 30% of cases that affects mainly immunocompromised persons. The aim of this research was to characterize L. monocytogenes pulsed-field gel electrophoresis (PFGE) types isolated from meat products collected at public markets in Araguaina city, TO. Sixty samples of raw ground beef and frescal sausage were analyzed during the second half of 2008. Five out of 30 samples (16.7%) of raw ground beef tested positive for L. monocytogenes, three of which were classified as serotype 1/2b and two as serotype 4b. Among the 30 samples of sausage collected, two strains of L. monocytogenes were isolated (6.7%), one of them belonging to serotype 1/2a and the other belonging to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pulsed field gel electrophoresis; Ground beef; Frescal sausage; L. monocytogenes.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000800016