Consulta: subjectFacets:"Quantitative RT-PCR"
Registros recuperados: 4
Data/hora: 17/06/2024 13:42:50

Expression profiles of sugarcane under drought conditions: Variation in gene regulation
Provedor de dados: 74
Autores: Andrade,Júlio César Farias de; Terto,Jackeline; Silva,José Vieira; Almeida,Cícero.
Abstract Drought is a major factor in decreased sugarcane productivity because of the resulting morphophysiological effects that it causes. Gene expression studies that have examined the influence of water stress in sugarcane have yielded divergent results, indicating the absence of a fixed pattern of changes in gene expression. In this work, we investigated the expression profiles of 12 genes in the leaves of a drought-tolerant genotype (RB72910) of sugarcane and compared the results with those of other studies. The genotype was subjected to 80–100% water availability (control condition) and 0–20% water availability (simulated drought). To analyze the physiological status, the SPAD index, Fv/Fm ratio, net photosynthesis (A), stomatal conductance (gs) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene expression; Plant breeding; Quantitative RT-PCR.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400465

Validation of reference genes for RT-qPCR analysis of CYP4T expression in crucian carp
Provedor de dados: 74
Autores: Mo,Fei; Zhao,Jie; Liu,Na; Cao,Li-hua; Jiang,Shan-xiang.
Reference genes are commonly used for normalization of target gene expression during RT-qPCR analysis. However, no housekeeping genes or reference genes have been identified to be stable across different tissue types or under different experimental conditions. To identify the most suitable reference genes for RT-qPCR analysis of target gene expression in the hepatopancreas of crucian carp (Carassius auratus) under various conditions (sex, age, water temperature, and drug treatments), seven reference genes, including beta actin (ACTB), beta-2 microglobulin (B2M), embryonic elongation factor-1 alpha (EEF1A), glyceraldehyde phosphate dehydrogenase (GAPDH), alpha tubulin (TUBA), ribosomal protein l8 (RPL8) and glucose-6-phosphate dehydrogenase (G6PDH), were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene expression; Quantitative RT-PCR; Housekeeping genes; CYP4T; Crucian carp.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400005

Differential expression of the pr1A gene in Metarhizium anisopliae and Metarhizium acridum across different culture conditions and during pathogenesis
Provedor de dados: 74
Autores: Leão,Mariele Porto Carneiro; Tiago,Patricia Vieira; Andreote,Fernando Dini; de Araújo,Welington Luiz; de Oliveira,Neiva Tinti.
The entomopathogenic fungi of the genus Metarhizium have several subtilisin-like proteases that are involved in pathogenesis and these have been used to investigate genes that are differentially expressed in response to different growth conditions. The identification and characterization of these proteases can provide insight into how the fungus is capable of infecting a wide variety of insects and adapt to different substrates. In addition, the pr1A gene has been used for the genetic improvement of strains used in pest control. In this study we used quantitative RT-PCR to assess the relative expression levels of the pr1A gene in M. anisopliae and M. acridum during growth in different culture conditions and during infection of the sugar cane borer,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Entomopathogen; Diatraea saccharalis; Quantitative RT-PCR; Expression pattern.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100086

Standard-curve competitive RT-PCR quantification of myogenic regulatory factors in chicken embryos
Provedor de dados: 56
Autores: Alvares,L.E.; Mantoani,A.; Corrente,J.E.; Coutinho,L.L.
The reverse transcription-polymerase chain reaction (RT-PCR) is the most sensitive method used to evaluate gene expression. Although many advances have been made since quantitative RT-PCR was first described, few reports deal with the mathematical bases of this technique. The aim of the present study was to develop and standardize a competitive PCR method using standard-curves to quantify transcripts of the myogenic regulatory factors MyoD, Myf-5, Myogenin and MRF4 in chicken embryos. Competitor cDNA molecules were constructed for each gene under study using deletion primers, which were designed to maintain the anchorage sites for the primers used to amplify target cDNAs. Standard-curves were prepared by co-amplification of different amounts of target cDNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quantitative RT-PCR; Standard-curve; Myogenic regulatory factors; Gene expression.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200004