Consulta: subjectFacets:"RDNA"
Registros recuperados: 39
Data/hora: 02/06/2024 04:38:35

Characterization of the nuclear ribosomal DNA unit in Oxalis tuberosa (Oxalidacea) and related species
Provedor de dados: 69
Autores: Tosto,Daniela; Hopp,Esteban.
Oxalis tuberosa is an octoploid Andean tuber crop called "oca" that belongs to the worldwide distributed genus Oxalis. The genus is very heterogeneous and its systematics is still problematic. It has been proposed that O. tuberosa evolved by polyploidization of a still not defined ancestor that belongs to an alliance of species sharing the same basic chromosome number (x = 8). Nuclear rDNA units of O. tuberosa and a selected group of four related diploid species were characterised by RFLP using different restriction endonucleases and southern hybridization probes to produce a restriction map for EcoRI and BamHI. The major rDNA unit length in O. tuberosa was estimated at 10.7 kbp. As expected, restriction site variation was observed mainly in the intergenic...
Tipo: Journal article
Palavras-chave: Andean crops; Oca; Oxalis; RDNA; RFLPs.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300002

High levels of chromosomal differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae)
Provedor de dados: 74
Autores: Moura,Rita de Cássia de; Melo,Natoniel Franklin de; Souza,Maria José de.
Euchroma gigantea was karyotypically studied using conventional staining, C-banding, silver nitrate staining and ribosomal fluorescent in situ hybridization (rDNA FISH). Broad wide autosomal polymorphism and a complex sex determination system were found in this beetle. Karyotype complements ranging from 2n = 32, X1X2X3Y1Y2Y3 to 2n = 36,X1X2X3Y1Y2Y3 were detected in the sample analyzed. Punctiform supernumerary chromosomes were present in the different karyotypes. The karyotypic evolution of Brazilian E. gigantea may have taken two directions, reduction in the diploid number of 2n = 36 to 24 through centric fusions or 2n = 24 to 36 due to chromosomal fissions. In addition, pericentric inversions were also involved. The complex multiple sex mechanism of this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromosome polymorphism; C-banding; Multiple sex system; NOR; RDNA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300007

Cytogenetic variation of repetitive DNA elements in Hoplias malabaricus (Characiformes - Erythrinidae) from white, black and clear water rivers of the Amazon basin
Provedor de dados: 74
Autores: Santos,Fabíola Araújo dos; Marques,Diego Ferreira; Terencio,Maria Leandra; Feldberg,Eliana; Rodrigues,Luís Reginaldo R.
Abstract Hoplias malabaricus is a common fish species occurring in white, black and clear water rivers of the Amazon basin. Its large distribution across distinct aquatic environments can pose stressful conditions for dispersal and creates possibilities for the emergence of local adaptive profiles. We investigated the chromosomal localization of repetitive DNA markers (constitutive heterochromatin, rDNA and the transposable element REX-3) in populations from the Amazonas river (white water), the Negro river (black water) and the Tapajós river (clear water), in order to address the variation/association of cytogenomic features and environmental conditions. We found a conserved karyotypic macrostructure with a diploid number of 40 chromosomes (20...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenomics; FISH; RDNA; REX 3; Tapajós river.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100040

Cytogenetic studies in six species of Scinax (Anura, Hylidae) clade Scinax ruber from northern and northeastern Brazil
Provedor de dados: 74
Autores: Nogueira,Lídia; Zanoni,Juliani Bruna; Solé,Mirco; Affonso,Paulo Roberto Antunes de Mello; Siqueira,Sérgio; Sampaio,Iracilda.
Scinax species are still underrepresented in cytogenetic studies, mainly with respect to populations from northeastern and northern Brazil. In this study, we provide new chromosomal information on Scinax boesemani, S. camposseabrai, S. garbei, S. pachycrus, S. trilineatus and S. x-signatus, all belonging to clade S. ruber. They were collected at two locations in the Caatinga biome (northeastern Brazil) and at one in the Amazon (northern Brazil) biomes. Chromosomes were analyzed by conventional staining, C-banding, Ag-NOR staining, and fluorochrome staining. All species shared a modal diploid value of 2n = 24 and fundamental arm number (FN) of 48. Moreover, both chromosomal size and morphology were similar to other species in this Scinax clade. C-banding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amphibians; Chromosomes; Fluorochromes; Heterochromatin; RDNA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200156

Efficiency of IRAP and ITS-RFLP marker systems in accessing genetic variation of Pyrenophora graminea
Provedor de dados: 74
Autores: Zein,Imad; Jawhar,Mohammed; Arabi,Mohammed Imad Eddin.
The usefulness of IRAP (inter-retrotransposon amplified polymorphism) and ITS-RFLP (restriction of PCR-amplified internal transcribed spacers of the rDNA) markers in the analysis of 39 Pyrenophora graminea isolates was determined. Each marker system could discriminate between all of the isolates in detecting polymorphism, albeit with variable efficiency. IRAP and ITS-RFLP produced 85% and 77% polymorphic bands, respectively, with a corresponding mean polymorphic information content (PIC) of 0.38 and 0.36. The IRAP marker index ratio (2.41) was higher than ITS-RFLP (1.50). On one hand, the quality nature of data (QND) was higher for ITS-RFLP (0.169) than IRAP (0.093). However, correlation between both marker similarity matrices was significant (r = 0.34, p...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pyrenophora graminea; IRAP; Internal transcribed spacer; RDNA.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200021

Identification and taxonomy of some entomopathogenic Paecilomyces spp. (Ascomycota) isolates using rDNA-ITS Sequences
Provedor de dados: 74
Autores: Inglis,Peter W.; Tigano,Myrian S.
A phylogenetic analysis of the 5.8S rDNA and internal transcribed spacer (ITS1 and ITS2) sequences from some entomogenous Paecilomyces species supports the polyphyly of the genus and showed the existence of cryptic species. In the Eurotiales, anamorphs Paecilomyces variotii and Paecilomyces leycettanus were related to the teleomorphs Talaromyces and Thermoascus. In the Hypocreales, three major ITS subgroups were found, one of which included Paecilomyces viridis, Paecilomyces penicillatus, Paecilomyces carneus and isolates identified as Paecilomyces lilacinus and Paecilomyces marquandii. However, the majority of the P. lilacinus and P. marquandii isolates formed a distinct and distantly related subgroup, while the other major subgroup contained Paecilomyces...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Paecilomyces spp.; Phylogenetic analysis; RDNA; Molecular taxonomy.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100025

Karyotype and genome size comparative analyses among six species of the oilseed-bearing genus Jatropha (Euphorbiaceae)
Provedor de dados: 74
Autores: Marinho,Anne C.T.A.; Vasconcelos,Santelmo; Vasconcelos,Emanuelle V.; Marques,Daniela A.; Benko-Iseppon,Ana Maria; Brasileiro-Vidal,Ana Christina.
Abstract Jatropha is an important genus of Euphorbiaceae, with species largely used for various purposes, including the manufacturing of soaps and pharmaceutical products and applications in the bioenergetic industry. Although there have been several studies focusing J. curcas in various aspects, the karyotype features of Jatropha species are poorly known. Therefore, we analyzed six Jatropha species through fluorochrome staining (CMA/DAPI), fluorescent in situ hybridization (FISH) with 5S and 45S rDNA probes and genome size estimation by flow cytometry. Our results revealed several chromosome markers by both CMA/DAPI and FISH for the analyzed species. Five Jatropha species (J. curcas, J. gossypiifolia, J. integerrima, J. multifida and J. podagrica) showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotaxonomy; DNA C-value; Heterochromatin; Physic nut; RDNA.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300442

Phylogenomic and single nucleotide polymorphism analyses revealed the hybrid origin of Spondias bahiensis (family Anacardiaceae): de novo genome sequencing and comparative genomics
Provedor de dados: 74
Autores: Nobre,Lydayanne Lilás de Melo; Santos,José Daniel Oliveira dos; Leite,Rychard; Almeida,Cícero.
Abstract The genus Spondias (family Anacardiaceae) comprises 19 taxa, ten of which occur in Neotropical regions. Spondias bahiensis has been suggested to be a hybrid, although initial evidence does not support this hypothesis. The aim of this study was to test the hypothesis of the hybrid origin of S. bahiensis using high-throughput sequencing with single nucleotide polymorphism (SNP) analysis, characterization of intragenomic nuclear ribosomal DNA (nrDNA), and nuclear and chloroplast phylogenomic analyses. The SNP analysis revealed a high number of SNPs in the S. bahiensis genome, and with respect to nrDNA, S. bahiensis shared approximately half of the SNP alleles with S. tuberosa, but not with S. mombin. Combining the SNP analysis with nrDNA phylogeny...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RDNA; Phylogenetics; Evolution; Hybrid offspring.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500878

Genetic analysis reveals candidate species in the Scinax catharinae clade (Amphibia: Anura) from Central Brazil
Provedor de dados: 74
Autores: Nogueira,Lídia; Solé,Mirco; Siqueira,Sérgio; Affonso,Paulo Roberto Antunes de Mello; Strüssmann,Christine; Sampaio,Iracilda.
Abstract Scinax (Anura: Hylidae) is a species-rich genus of amphibians (113 spp.), divided into five species groups by morphological features. Cladistic analyses however revealed only two monophyletic clades in these groups: Scinax catharinae and Scinax ruber. Most species from the S. catharinae clade are found in Atlantic rainforest, except for Scinax canastrensis,S. centralis, S. luizotavioi, S. machadoi,S. pombali and S. skaios. In the present work, specimens of Scinax collected in Chapada dos Guimarães, central Brazil, were morphologically compatible with species from theS. catharinae group. On the other hand, genetic analysis based on mitochondrial (16S and 12S) and nuclear (rhodopsin) sequences revealed a nucleotide divergence of 6 to 20% between...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Amphibians; DNA barcode; Cerrado; RDNA.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100049

Cytogenetic analyses of two endemic fish species from the São Francisco River basin: Conorhynchus conirostris and Lophiosilurus alexandri (Siluriformes)
Provedor de dados: 74
Autores: Marques,Marilza Barbosa de Almeida; Moreira-Filho,Orlando; Garcia,Caroline; Margarido,Vladimir Pavan.
Two Siluriformes species endemic to the São Francisco River basin were characterized by conventional and differential cytogenetic analyses involving C-banding, Ag-nucleolar organizer region (NOR) and chromomycin A3 (CMA3) staining, and FISH (fluorescent in situ hybridization) with 18S and 5S rDNA probes. Conorhynchus conirostris presents a higher diploid number (2n = 60) than those detected in Pimelodidae representatives, whereas Lophiosilurus alexandri, with a karyotype of 2n = 54 chromosomes, presents a chromosomal constitution similar to that found in the family Pseudopimelodidae. Plesiomorphic characteristics such as single NORs at terminal positions are found in both species, as revealed by CMA3 and silver nitrate staining, and FISH with a 18S rDNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Incertae sedis; Pimelodidae; Pseudopimelodidae; RDNA; Siluriformes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200008

Chromosome mapping of ribosomal genes and histone H4 in the genus Radacridium (Romaleidae)
Provedor de dados: 74
Autores: Anjos,Allison; Loreto,Vilma; Souza,Maria José de.
In this study, two species of Romaleidae grasshoppers, Radacridium mariajoseae and R.nordestinum, were analyzed after CMA3/DA/DAPI sequential staining and fluorescence in situ hybridization (FISH) to determine the location of the 18S and 5S rDNA and histone H4 genes. Both species presented karyotypes composed of 2n = 23, X0 with exclusively acrocentric chromosomes. CMA3+ blocks were detected after CMA3/DA/DAPI staining in only one medium size autosome bivalent and in the X chromosome in R. mariajoseae. On the other hand, all chromosomes, except the L1 bivalent, of R. nordestinum presented CMA3+ blocks. FISH analysis showed that the 18S genes are restricted to the X chromosome in R. mariajoseae, whereas these genes were located in the L2,S9 and S10...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytogenetics; FISH; Fluorochromes; Grasshoppers; RDNA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300006

Sequence analysis of the rDNA intergenic spacer of Metarhizium strains isolated in Brazil
Provedor de dados: 74
Autores: Yanaka-Schäfer,Fabiana Y.; Dall'Onder,Leonara P.; Panichi,Mariana C.; Mendes,Roberta G.; Fagundes,Nelson J.R.; Bandinelli,Josiane B.; Bogo,Maurício R.
To assess the extent of genetic variability of rDNA intergenic spacer (IGS) in Metarhizium sp., 34 strains (27 isolated in Brazil) were sequenced and analyzed together with an additional 20 Metarhizium anisopliae var. anisopliae sequences retrieved from GenBank. Overall, the global nucleotide diversity for the region under study was of 0.090, while for the Brazilian isolates it was only 0.016. Phylogenetic analyses showed four well-supported groups (A, B, C, and D), one of which (D) has not been previously identified. All but one of the Brazilian strains cluster in this novel D phylogroup, suggesting that the genetic variation found in Brazil is a subset of the worldwide M. anisopiliae var. anisopliae variation.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Intergenic spacer; Metarhizium; RDNA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100020

Differential transcription of ribosomal cistrons denoting nucleolar dominance in hybrids of Drosophila mulleri and Drosophila navojoa (mulleri complex, Repleta group)
Provedor de dados: 74
Autores: Commar,Leliane Silva; Bicudo,Hermione E.M.C.; Rahal,Paula; Ceron,Carlos Roberto.
The fruit flies Drosophila mulleri and Drosophila navojoa are included in the mulleri complex of the mulleri subgroup and Repleta group. Although there is no demonstration that interspecific crosses between them occur in nature, they intercross in the laboratory in both cross directions. Previous data have shown the occurrence of nucleolar dominance in interspecific hybrids of some species in the mulleri complex. We investigated nucleolar dominance in D. mulleri/D. navojoa hybrids using the transcription profiles of the rDNA internal transcribed spacer (ITS-1) region. The results showed that the ribosomal cistrons present in the X chromosome and in the microchromosome of D. navojoa are exclusively or preferentially transcribed in these hybrids depending on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drosophila mulleri; D. navojoa; Interspecific hybrids; Nucleolar dominance; RDNA.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600028

Diferenciação de biótipos de Bemísia tabaci utilizando PCR-RFLP e sequenciamento da região ITS1 rDNA.
Provedor de dados: 14
Autores: RABELLO, A. R.; QUEIROZ, P. R.; SIMÕES, K. C.; HIRAGI, C. O.; LIMA, L. H. C.; OLIVEIRA, M. R. V.; MEHTA, A.
bitstream/CENARGEN/26632/1/bp095.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Biótipos; PCR-RFLP; ITS1; RDNA; Biotype.; Bemisia Tabaci; Mosca Branca.; Bemisia.
Ano: 2005
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/187042

Phylogenetic position and taxonomy of Cycloseris explanulata and C. wellsi (Scleractinia: Fungiidae): lost mushroom corals find their way home
Provedor de dados: 5
Autores: Benzoni, Francesca; Arrigoni, Roberto; Stefani, Fabrizio; Reijnen, Bastian T.; Montano, Simone; Hoeksema, Bert W.
The scleractinian species Psammocora explanulata and Coscinaraea wellsi were originally classified in the family Siderastreidae, but in a recent morpho-molecular study it appeared that they are more closely related to each other and to the Fungiidae than to any siderastreid taxon. A subsequent morpho-molecular study of the Fungiidae provided new insights regarding the phylogenetic relationships within that family. In the present study existing molecular data sets of both families were analyzed jointly with those of new specimens and sequences of P. explanulata and C. wellsi. The results indicate that both species actually belong to the Cycloseris clade within the family Fungiidae. A reappraisal of their morphologic characters based on museum specimens and...
Tipo: Text
Palavras-chave: COI; Coscinaraea wellsi; Psammocora explanulata; RDNA.
Ano: 2012
URL: https://archimer.ifremer.fr/doc/00145/25580/23718.pdf

Prorocentrum bimaculatum sp nov (Dinophyceae, prorocentrales), a new benthic dinoflagellate species from Kuwait (Arabian gulf)
Provedor de dados: 5
Autores: Chomerat, Nicolas; Saburova, Maria; Bilien, Gwenael; Al-yamani, Faiza.
A new benthic dinoflagellate species, Prorocentrum bimaculatum sp. nov., is studied from Kuwaits marine sediments, based on detailed morphological and molecular data. Cells are large, oblong oval in shape. They are 49.955.3 mu m long and 38.443.2 mu m wide. The ornamentation of this new species is peculiar, and characterized by smooth valves with large pores (0.320.50 mu m) scattered on their surface, except in two circular patches of similar to 15 mu m in diameter, devoid of ornamentation and located on both sides of the valve centers. The periflagellar area is widely triangular, located in a moderate excavation of the right valve, and comprises nine platelets. The intercalary band of P. bimaculatum is smooth. The molecular phylogenetic position of this...
Tipo: Text
Palavras-chave: Arabian Gulf; Benthic dinoflagellates; Kuwait; Molecular phylogeny; Morphology; Nuclear DNA; Prorocentrum; RDNA; SEM; Taxonomy.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00066/17756/15698.pdf

Madanidinium loirii gen. et sp nov. (Dinophyceae), a new marine benthic dinoflagellate from Martinique Island, Eastern Caribbean
Provedor de dados: 5
Autores: Chomerat, Nicolas; Bilien, Gwenael.
A new benthic phototrophic dinoflagellate is described from sediments of a tropical marine cove at Martinique Island and its micromorphology is studied by means of light and electron microscopy. The cell contains small golden-brown chloroplasts and the oval nucleus is posterior. It is laterally compressed, almost circular in shape when viewed laterally. It consists of a small epitheca tilted toward the right lateral side and a larger hypotheca. In the left view, the cingulum is more anterior and the epitheca is reduced. The cingulum is displaced and left-handed. This organism is peculiar in having no apical pore and its thecal plate arrangement is 2 ' 1a 7 '' 5c 3s 5 ''' 1 ''''. The plates are smooth with small groups of pores scattered on their surface....
Tipo: Text
Palavras-chave: Caribbean; Benthic; Dinophyceae; SEM; Phylogeny; Morphology; Taxonomy; Martinique; RDNA.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00195/30622/29458.pdf

Prorocentrum shikokuense Hada and P. donghaiense Lu are junior synonyms of P. obtusidens Schiller, but not of P. dentatum Stein (Prorocentrales, Dinophyceae)
Provedor de dados: 5
Autores: Shin, Hyeon Ho; Li, Zhun; Mertens, Kenneth; Seo, Min Ho; Gu, Haifeng; Lim, Weol Ae; Yoon, Yang Ho; Soh, Ho Young; Matsuoka, Kazumi.
In Japanese, Chinese and Korean coastal waters, recurrent blooms of a small, elongate Prorocentrum species have been observed in recent years. In previous studies, this species has been respectively identified as P. shikokuense, P. donghaiense and P. dentatum, despite morphological similarity and identical rDNA sequences. To resolve the confusion, morphological features, including the architectural details of the periflagellar area, were examined and ribosomal DNA (rDNA) sequences were obtained from specimens collected from the East China Sea and Korean coast, and a strain established in the waters off the Canary Islands of Spain. In addition, the descriptions of the three species and allied species were reviewed. Morphological observations and a phylogeny...
Tipo: Text
Palavras-chave: Morphology; Periflagellar area; RDNA; Prorocentrum veloi; P. monacense.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00566/67839/69555.pdf

Taxonomy and phylogenetic relationships of the coral genera Australomussa and Parascolymia (Scleractinia, Lobophylliidae)
Provedor de dados: 5
Autores: Arrigoni, Roberto; Richards, Zoe T.; Chen, Chaolun Allen; Baird, Andrew H.; Benzoni, Francesca.
Novel micromorphological characters in combination with molecular studies have led to an extensive revision of the taxonomy and systematics of scleractinian corals. In the present work, we investigate the macro- and micromorphology and the phylogenetic position of the genera Australomussa and Parascolymia, two monotypic genera ascribed to the family Lobophylliidae. The molecular phylogeny of both genera was addressed using three markers, the partial mitochondrial COI gene and the nuclear histone H3 and the ribosomal ITS region. Based on molecular data, Australomussa and Parascolymia belong to the Lobophylliidae and they cluster together with the genera Lobophyllia and Symphyllia within the same clade. While A. rowleyensis and P. vitiensis are closely...
Tipo: Text
Palavras-chave: COI; Evolution; Histone H3; Lobophyllia; Pacific Ocean; RDNA; Symphyllia; Systematics; Taxonomic revision.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00298/40912/39970.pdf

Molecular systematics of the cotton root rot pathogen, Phymatotrichopsis omnivora
Provedor de dados: 16
Autores: Marek, S.M.; Hansen, K.; Romanish, M.; Thorn, R.G.
Cotton root rot is an important soilborne disease of cotton and numerous dicot plants in the south-western United States and Mexico. The causal organism, Phymatotrichopsis omnivora (= Phymatotrichum omnivorum), is known only as an asexual, holoanamorphic (mitosporic) fungus, and produces conidia resembling those of Botrytis. Although the corticoid basidiomycetes Phanerochaete omnivora (Polyporales) and Sistotrema brinkmannii (Cantharellales; both Agaricomycetes) have been suggested as teleomorphs of Phymatotrichopsis omnivora, phylogenetic analyses of nuclear small- and large-subunit ribosomal DNA and subunit 2 of RNA polymerase II from multiple isolates indicate that it is neither a basidiomycete nor closely related to other species of Botrytis...
Tipo: Article / Letter to the editor
Palavras-chave: Ozonium; Pezizales; Phylogeny; Phymatotrichum root rot; Pulchromyces fimicola; RDNA; RPB2; Texas.
Ano: 2009
URL: http://www.repository.naturalis.nl/record/531880

Plectosphaerella species associated with root and collar rots of horticultural crops in southern Italy
Provedor de dados: 16
Autores: Carlucci, A.; Raimondo, M.L.; Santos, J.; Phillips, A.J.L.
Plectosphaerella cucumerina, most frequently encountered in its Plectosporium state, is well known as a pathogen of several plant species causing fruit, root and collar rot, and collapse. It is considered to pose a serious threat to melon (Cucumis melo) production in Italy. In the present study, an intensive sampling of diseased cucurbits as well as tomato and bell pepper was done and the fungal pathogens present on them were isolated. Phylogenetic relationships of the isolates were determined through a study of ribosomal RNA gene sequences (ITS cluster and D1/D2 domain of the 28S rRNA gene). Combining morphological, culture and molecular data, six species were distinguished. One of these (Pa. cucumerina) is already known. Four new species are described as...
Tipo: Article / Letter to the editor
Palavras-chave: D1/D2; ITS; LSU; Phylogeny; Plectosporium; RDNA; Systematics; Taxonomy.
Ano: 2012
URL: http://www.repository.naturalis.nl/record/531914

The phylogenetic position of Artematopodidae (Coleoptera: Elateroidea), with description of the first two Eurypogon species from China
Provedor de dados: 16
Autores: Kundrata, R.; Bocakova, M.; Bocak, L.
The Artematopodidae is a species-poor beetle family with contentious relationships to byrrhoid and elateroid families. Recent molecular phylogenetic analyses brought ambiguous results based on a single sequenced species. We investigated the taxonomic placement of Artematopodidae within Elateriformia using ribosomal (18S, 28S) and mitochondrial (rrnL, cox1) molecular markers and three artematopodid species. Our analyses placed Artematopodidae close to Omethidae+Telegeusidae in a basal position of broadly defined Elateroidea. Additionally, we described the first artematopodid species from China – Eurypogon jaechi sp. nov. and E. heishuiensis sp. nov. These species are reported from mountains of Yunnan and can be easily distinguished from their Palaearctic...
Tipo: Article / Letter to the editor
Palavras-chave: MtDNA; RDNA; Diversity; Elateriformia; New species; Palaearctic Region; Phylogeny; Taxonomy; 42.75; 42.64.
Ano: 2013
URL: http://www.repository.naturalis.nl/record/462591

A new species of bird’s nest fungi: characterisation of Cyathus subglobisporus sp. nov. based on morphological and molecular data
Provedor de dados: 16
Autores: Zhao, R.-L.; Desjardin, D.E.; Soytong, K.; Hyde, K.D.
Recent collections of bird’s nest fungi (i.e. Crucibulum, Cyathus, Mycocalia, Nidula, and Nidularia species) in northern Thailand resulted in the discovery of a new species of Cyathus, herein described as C. subglobisporus. This species is distinct by a combination of ivory-coloured fruiting bodies covered with shaggy hairs, plications on the inner surface of the peridium and subglobose basidiospores. Phylogenetic analyses based on ITS and LSU ribosomal DNA sequences using neighbour-joining, maximum likelihood and weighted maximum parsimony support Cyathus subglobisporus as a distinct species and sister to a clade containing C. annulatus, C. renweii and C. stercoreus in the Striatum group.
Tipo: Article / Letter to the editor
Palavras-chave: Bird’s nest fungi; Gasteromycetes; New species; Phylogeny; RDNA.
Ano: 2008
URL: http://www.repository.naturalis.nl/record/532073

Phylogenetic position and taxonomy of Cycloseris explanulata and C. wellsi (Scleractinia: Fungiidae): lost mushroom corals find their way home
Provedor de dados: 16
Autores: Benzoni, F.; Arrigoni, R.; Stefani, F.; Reijnen, B.T.; Montano, S.; Hoeksema, B.
The scleractinian species Psammocora explanulata and Coscinaraea wellsi were originally classified in the family Siderastreidae, but in a recent morpho-molecular study it appeared that they are more closely related to each other and to the Fungiidae than to any siderastreid taxon. A subsequent morpho-molecular study of the Fungiidae provided new insights regarding the phylogenetic relationships within that family. In the present study existing molecular data sets of both families were analyzed jointly with those of new specimens and sequences of P. explanulata and C. wellsi. The results indicate that both species actually belong to the Cycloseris clade within the family Fungiidae. A reappraisal of their morphologic characters based on museum specimens and...
Tipo: Article / Letter to the editor
Palavras-chave: COI; Coscinaraea wellsi; Psammocora explanulata; RDNA; 42.72.
Ano: 2012
URL: http://www.repository.naturalis.nl/record/421558

Taxonomy and phylogenetic relationships of the coral genera Australomussa and Parascolymia (Scleractinia, Lobophylliidae)
Provedor de dados: 16
Autores: Arrigoni, R.; Richards, Z.T.; Chen, C.A.; Baird, A.H.; Benzoni, F.
Novel micromorphological characters in combination with molecular studies have led to an extensive revision of the taxonomy and systematics of scleractinian corals. In the present work, we investigate the macro- and micromorphology and the phylogenetic position of the genera Australomussa and Parascolymia, two monotypic genera ascribed to the family Lobophylliidae. The molecular phylogeny of both genera was addressed using three markers, the partial mitochondrial COI gene and the nuclear histone H3 and the ribosomal ITS region. Based on molecular data, Australomussa and Parascolymia belong to the Lobophylliidae and they cluster together with the genera Lobophyllia and Symphyllia within the same clade. While A. rowleyensis and P. vitiensis are closely...
Tipo: Article / Letter to the editor
Palavras-chave: COI; Evolution; Histone H3; Lobophyllia; Pacific Ocean; RDNA; Symphyllia; Systematics; Taxonomic revision; 42.72.
Ano: 2014
URL: http://www.repository.naturalis.nl/record/499033

Phylogenetic and morphological assessment of two new species of Amniculicola and their allies (Pleosporales)
Provedor de dados: 16
Autores: Zhang, Y.; Fournier, J.; Crous, P.W.; Pointing, S.B.; Hyde, K.D.
Two new species of Amniculicola, A. immersa sp. nov. and A. parva sp. nov. from submerged wood in a freshwater environment in Denmark and France are respectively described and illustrated. In addition, partial 28S rDNA sequence data is analysed to investigate their phylogenetic relationships with other pleosporalean taxa. All presently known Amniculicola species, A. immersa, A. lignicola and A. parva, form a robust clade together with the anamorphic species Anguillospora longissima, Spirosphaera cupreorufescens and Repetophragma ontariense. These six species, which are all from freshwater and mostly from Europe, constitute a well-supported group containing Pleospora rubicunda and Massariosphaeria typhicola. This putative monophyletic assemblage may...
Tipo: Article / Letter to the editor
Palavras-chave: Anamorphs; Freshwater fungi; Phylogeny; Pseudoparaphyses; RDNA.
Ano: 2009
URL: http://www.repository.naturalis.nl/record/531919

Molecular Tool for Identification of Closely Related Species of Trichogramma (Hymenoptera: Trichogrammatidae): T. rojasi Nagaraja &amp;amp; Nagarkatti and T. lasallei Pinto
Provedor de dados: 82
Autores: CIOCIOLA JR.,AMÉRICO I.; QUERINO,RANYSE B.; ZUCCHI,ROBERTO A.; STOUTHAMER,RICHARD.
The sequence of the ITS2 (internally transcribed spacer 2) region of the rDNA was used to identify two closely related Trichogramma species: T. rojasi and T. lasallei, the second species recently reported in Brazil. The DNA of the samples was extracted using Chelex 100. The PCR product was linked to a pGEM-T® vector and both were then placed into Petri dishes to grow white colonies, which demonstrate the correct ligation between the vector and the DNA. From these colonies, 10µl were purified and sequenced using a special kit, and the sequences aligned using the ESEE 3.0 software. Specific primers were efficient to amplify the ITS2 rDNA region of the Brazilian Trichogramma. The sequence of each species differed in length and in nucleotide position, showing...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Insecta; ITS2 region; Biological control; RDNA; Parasitoids.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2001000400010

Chromosomal characterization of the bonytongue Arapaima gigas (Osteoglossiformes: Arapaimidae)
Provedor de dados: 83
Autores: Marques,Debora Karla; Venere,Paulo Cesar; Galetti Junior,Pedro Manoel.
The mitotic chromosomes of the pirarucu Arapaima gigas inhabiting the middle Araguaia River and collected in the municipality of Araguaiana (MT, Brazil) were studied. The chromosomes were analyzed through Giemsa staining, C-banding, Ag-NOR staining and in situ hybridization using an 18S rRNA gene probe. The karyotype had 2n=56 comprising 14 biarmed and 14 uniarmed chromosome pairs in both sexes. No cytologically distinguishable sex chromosome was identified. A single NOR-bearing chromosome pair was detected by Ag-NOR staining and confirmed by 18S rDNA- FISH. Faint constitutive heterochromatin was C-banded in the centromeric region of some chromosomes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pirarucu; Karyotype; RDNA; Heterochromatin.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252006000200007

Cytogenetic analysis of Astyanax laticeps (Cope, 1894) (Ostariophysi: Characidae) from the laguna dos Patos system
Provedor de dados: 83
Autores: Rosa,Renata da; Rubert,Marceléia; Malabarba,Luiz R.; Martins-Santos,Isabel Cristina; Giuliano-Caetano,Lucia.
The genus Astyanax is prominent among Characiformes, due to the large number of species found and its wide geographic distribution. In this work, Astyanax laticeps specimens from the laguna dos Patos system were cytogenetically analyzed. A diploid number of 2n = 50 chromosomes distributed into 6m+16sm+16st+12a (FN = 88) was found, without differences between males and females. A few small heterochromatin blocks were observed, besides three more conspicuous C-bands, corresponding to NORs, as confirmed by silver nitrate and CMA3 staining, FISH, and DAPI negative staining. These regions were located in a medium-sized subtelocentric and in a large subtelocentric chromosomal pair, probably because of a deletion of this region in one homologous chromosome, or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Astyanax scabripinnis species complex; RDNA; Homologous transposition.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000400007

Karyotype analysis of three species of Corydoras (Siluriformes: Callichthyidae) from southern Brazil: rearranged karyotypes and cytotaxonomy
Provedor de dados: 83
Autores: Barbosa,Patrícia; Pucci,Marcela B.; Nogaroto,Viviane; Almeida,Mara C.; Artoni,Roberto F.; Vicari,Marcelo R.
ABSTRACT The genus Corydoras comprises a diversity of species with different diploid numbers. We compared cytogenetic data among Corydoras species from different rivers of the Ponta Grossa Arch region in southern Brazil. Corydoras ehrhardti and C. aff. paleatus have a similar karyotype formula and the same diploid number (2n = 44). Corydoras lacrimostigmata has a higher diploid number, with 2n = 58 chromosomes. Fluorescence in situ hybridization using 5S and 18S ribosomal DNA probes suggests that these ribosomal DNA sequences are involved in chromosomal rearrangements in these Corydoras species. 5S rDNA is a chromosomal marker that is considered to be unique to the species analyzed in this study. Signals of interstitial telomeric sites are seen in a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytosystematics; Heterokaryotypes; Karyotype description; RDNA; Vicariance.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000100222

Cytogenetic analysis of Baryancistrus xanthellus (Siluriformes: Loricariidae: Ancistrini), an ornamental fish endemic to the Xingu River, Brazil
Provedor de dados: 83
Autores: Medeiros,Larissa A.; Ginani,Eduardo G.; Sousa,Leandro M.; Py-Daniel,Lúcia H. Rapp; Feldberg,Eliana.
ABSTRACT Baryancistrus xanthellus is a species from the Ancistrini tribe known commonly as "amarelinho " or "golden nugget pleco". It is one of the most popular and valued ornamental fishes due to its color pattern. Also, it is an endemic species from the Xingu River occurring from Volta Grande do Xingu, region where the Belo Monte Hydropower Dam is being built, to São Félix do Xingu. The current study aimed to cytogenetically characterize B. xanthellus . Results point to the maintenance of 2n=52, which is considered the most common condition for the tribe, and a single nucleolus organizer region (NOR). Mapping of the 18S rDNA confirmed the NOR sites, and the 5S rDNA was mapped in the interstitial position of a single chromosome pair. The 18S and 5S rDNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FISH; Habitat endangered; RDNA; Volta Grande do Xingu.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000200212

Microstructural chromosome reorganization in the genus Trichomycterus (Siluriformes: Trichomycteridae)
Provedor de dados: 83
Autores: Oliveira,Maria Lígia M. de; Utsunomia,Ricardo; Pansonato-Alves,José Carlos; Scacchetti,Priscilla C.; Primo,Cleberson C.; Vicari,Marcelo R.; Artoni,Roberto F.; Centofante,Liano; Moreira-Filho,Orlando; Oliveira,Claudio; Foresti,Fausto.
Trichomycterus is a specious fish genus within Trichomycterinae and displays remarkable karyotype diversity. However, knowledge about their genomic structure and location of repetitive sequence is still limited. In order to better understand the karyotype diversification, we analyzed nine species of Trichomycterus using classical and molecular cytogenetic techniques. Results revealed a conserved diploid chromosome number of 2n=54 chromosomes in all analyzed species, although remarkable differences on the constitutive heterochromatin distribution were observed. In addition, while the 18S rDNA showed a conserved distribution pattern, the 5S rDNA sites showed a quite diverse location considering the analyzed species. Remarkably, both ribosomal genes were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype diversity; Repetitive DNA; RDNA; Supernumerary chromosomes.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000200211

Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential
Provedor de dados: 96
Autores: Maia,Natália da Costa; Souza,Patrícia Nirlane da Costa; Godinho,Bárbara Temponi Vilarino; Moreira,Silvino Intra; Abreu,Lucas Magalhães de; Jank,Liana; Cardoso,Patrícia Gomes.
ABSTRACT This study aimed to isolate and identify endophytic fungi from the forage grass P. maximum and evaluate their ability to inhibit the growth of plant pathogenic fungi. One sample from P. purpureum grass was also included. Surface disinfected stem fragments were used for endophytic fungal isolation. One hundred and twenty-six endophytic fungi were isolated, of which 118 were from P. maximum and eight from P. purpureum. Morphological characteristics and internal transcribed spacer (ITS) and 18S (NS) sequence comparisons identified most isolated endophytic fungi as belonging to the phylum Ascomycota, with Sarocladium being the dominant genus. The isolates were subjected to in vitro antagonism tests against pathogenic fungi, and 31 endophytic fungi...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antagonism; Grasses; Molecular identification; Plant pathogens; RDNA.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100303

Morphological and molecular characterization of Puccinia kuehnii, the causal agent of sugarcane orange rust, in Cub
Provedor de dados: 63
Autores: Perera,María Francisca; Bertani,Romina Priscila; Arias,Marta Eugenia; Hechavarría,María de la Luz La O; Navarro,María de los Ángeles Zardón; Debes,Mario Alberto; Luque,Ana Catalina; Cuenya,María Inés; Rojas,Ricardo Acevedo; Castagnaro,Atilio Pedro.
ABSTRACT Sugarcane orange rust caused by Puccinia kuehnii has recently become an important disease in sugarcane crops and its spread is causing great concern to growers. In this study, we analyzed spores from symptomatic orange rust sugarcane leaves collected in multiple locations in Cuba in a 4-year-period in order to characterize morphological traits of P. kuehnii, establish an adequate molecular technique to characterize it, and determine its infection court in sugarcane. Orange rust caused by P. kuehnii was confirmed by polymerase chain reaction (PCR) and morphological characterization. AFLP markers detected high diversity in P. kuenhnii samples. Sequencing of rDNA regions, as expected, did not reveal differences and SSR markers designed for P....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; RDNA; SSR; Infection courts.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000201200

Two new species of the industrially relevant genus Absidia (Mucorales) from soil of the Brazilian Atlantic Forest
Provedor de dados: 39
Autores: Cordeiro,Thalline Rafhaella Leite; Nguyen,Thuong Thuong Thi; Lima,Diogo Xavier; Silva,Suzana Brito Gomes da; Lima,Catarina Ferreira de; Leitão,Joana D’Arc Alves; Gurgel,Luciana Melo Sartori; Lee,Hyang Burm; Santiago,André Luiz Cabral Monteiro de Azevedo.
ABSTRACT During a survey of Mucorales in soil from an upland forest area in Pernambuco, Brazil, two specimens were isolated and characterized based on their morphological, physiological, and molecular data (ITS and LSU rDNA). Phylogenetic analyses of the isolates revealed that the strains URM 8209 and URM 8210 are closely related to species of Absidia. URM 8209 forms conical, subglobose, and strawberry-shaped columellae and the sporangiospores are cylindrical and ellipsoid. URM 8210 produces hemispheric, subglobose, and strawberry-shaped columellae and the sporangiospores are globose, subglobose, ellipsoid, and short cylindrical. Based on evidence obtained through analysis of datasets (LSU and ITS rDNA regions), A. saloaensis sp. nov. (URM 8209) and A....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cunninghamellaceae; Mucoromyceta; RDNA; Soil; Taxonomy.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000300549

Karyotype characterization and evolution of chromosome number in Cactaceae with special emphasis on subfamily Cactoideae
Provedor de dados: 39
Autores: Castro,Juliana P.; Moraes,Ana Paula; Chase,Mark W.; Santos,Angeline M. S.; Batista,Fabiane R. C.; Felix,Leonardo P.
ABSTRACT Cactaceae species are karyotypically well-known with x = 11 and chromosome number variation due mainly to polyploidization. However, both assumptions are based on descriptive observations without taking an evolutionary framework of Cactaceae into account. Aiming to confirm these hypotheses in an evolutionary context, we obtained chromosome numbers for 20 species of Cactoideae, performed an extensive review of chromosome number for the family, and analyzed these data using a phylogenetic approach. The karyotypes presented here were characterized by CMA/DAPI banding, and for six species 5S and 45S rDNA sites were located. Our data, along with a survey of the literature, reinforce the long-standing hypothesis of a x = 11 as the base chromosome number...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Base chromosome number; CMA/DAPI; Dysploidy; Heterochromatin; Polyploidy; RDNA.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000100135

Isolamento e caracterização parcial de sequências homólogas a genes ribossomais (rDNA) em Blastocladiella emersonii - DOI: 10.4025/actascibiolsci.v25i2.2037
Provedor de dados: 3
Autores: Gasques, Luciano Seraphim; Universidade Paranaense; Fernandez, Maria Aparecida; UEM; Correa, Luiz Carlos; UEM.
A definição e a caracterização de regiões de origens de replicação nos eucariotos superiores são ainda controversas. A iniciação da replicação é sítio-específica em alguns sistemas e, em outros, parece estar contida em regiões extensas. Regiões rDNA são modelos atrativos para o estudo de origens de replicação pela sua organização in tandem, reduzindo a área de estudo para o espaço restrito que codifica uma unidade de transcrição. Neste trabalho nós isolamos e caracterizamos parcialmente um clone que contém uma sequência ribossomal do fungo aquático Blastocladiella emersonii, Be97M20. Southern blots mostraram diversos sítios para enzimas de restrição Eco RI, HindIII e SalI. Northern blot de RNA total hibridado contra uma sonda feita com Be97M20 confirmou a...
Palavras-chave: 2.01.00.00-0    Biologia Geral Blastocladiella emersonii; RDNA; Genes ribossomais 2.01.00.00-0    Biologia Geral.
Ano: 2003
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2037

Genomic organization of the rDNA cistron of the teleost fish Cyprinus carpio
Provedor de dados: 48
Autores: VERA,MARÍA INÉS; MOLINA,ALFREDO; PINTO,RODRIGO; REYES,MAURICIO; ÁLVAREZ,MARCO; KRAUSKOPF,ERWIN; QUEZADA,CLAUDIA; TORRES,JORGE; KRAUSKOPF,MANUEL.
The seasonal adaptation of the teleost Cyprinus carpio to the cyclical changes of its habitat demands physiological compensatory responses. The process involves profound nucleolar adjustments and remarkable changes in rRNA synthesis, which affects ribosomal biosynthesis. In this context, we have demonstrated that the synthesis of several proteins involved in ribosomal biogenesis as protein kinase CK2, ribosomal protein L41 and nucleolin, as well as U3 snoRNP, are differentially regulated in summer-acclimatized carp compared to the cold-season adapted fish. To understand the mechanisms involved in the seasonal regulation of rRNA gene transcription, we have been studying the carp rDNA cistron structure. Because the cis-elements that regulate the expression...
Tipo: Journal article
Palavras-chave: Ribosomal gene; RRNA; RDNA; Fish; Carp.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200014

Colletotrichum isolates related to Anthracnose of cashew trees in Brazil: morphological and molecular description using LSU rDNA sequences
Provedor de dados: 52
Autores: Lopez,Ana Maria Queijeiro; Lucas,John Alexander.
Thirty six isolates of fungi obtained from anthracnose lesions of cashew and associated host plants in Brazil, were compared by their cultural, morphological and partial sequences of the 28S ribosomal DNA characters. They showed a high degree of cultural variability. The average mycelial growth rate on all tested media ranged from 10.2-13.3 mm/day between the isolates. Most of them produced perithecia (sterile and fertile) and some produced setae (sterile and fertile). All the isolates produced acervuli with predominantly cylindrical conidia (12.4-17.7 µmX 4.8-6.0 µm in width) with round ends, which became septate on germination, and produced unlobed or slightlylobed appressoria. Comparison of the D2 domain of the large subunit (LSU) rDNA sequences with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anthracnose; Inflorescence blight; Colletotrichum; RDNA; Cashew; Anacardium occidentale.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400001