Consulta: subjectFacets:"RNA"
Registros recuperados: 22
Data/hora: 14/06/2024 06:28:44

Comparison of commercially-available RNA extraction methods for effective bacterial RNA isolation from milk spiked samples
Provedor de dados: 69
Autores: García-Nogales,Paula; Serrano,Alicia; Secchi,Sonia; Gutiérrez,Serafín; Arís,Anna.
Nucleic-acid based methods for bacterial identification are extremely useful in diagnostic applications due to their specificity and sensitivity. However, they require an optimal purification of the target molecules. As part of the development of a new diagnostic method for the detection of bacterial RNA in cow milk, we have compared four commercially available RNA extraction kits for the isolation of bacterial RNA from spiked UHT milk samples. The kits were compared in terms of extraction efficiency and RNA purity using two bacterial species, the Gram negative Escherichia coli and the Gram positive Staphylococcus aureus. Two kits are based in silica-matrix extraction, and the other two in the guanidinium thiocyanate-phenol-chloroform extraction. In our...
Tipo: Journal article
Palavras-chave: Bacteria; Milk; Purification; RNA.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500019

Differential gene expression profiles in peripheral blood in Northeast Chinese Han people with acute myocardial infarction
Provedor de dados: 74
Autores: Fan,Lin; Meng,Heyu; Guo,Xudong; Li,Xiangdong; Meng,Fanbo.
Abstract This study aimed to use gene chips to investigate differential gene expression profiles in the occurrence and development of acute myocardial infarction (AMI). The study included 12 AMI patients and 12 healthy individuals. Total mRNA of peripheral bloodwas extracted and reversed-transcribed to cDNA for microarray analysis. After establishing two pools with three subjects each (3 AMI patients and 3 healthy individuals), the remaining samples were used for RT-qPCR to confirm the microarray data. From the microarray results, seven genes were randomly selected for RT-qPCR. RT-qPCR results were analyzed by the 2-ΔΔCt method. Microarray analysis showed that 228 genes were up- regulated and 271 were down-regulated (p ≤ 0.05, |logFC| &gt; 1). Gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute myocardial infarction; RNA; Differential expression.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100059

Биохимические эффекты полихлорированных бифенилов на организм черноморского моллюска Mytilus galloprovincialis Lam.
Provedor de dados: 13
Autores: Солдатов, А. А.; Бочко, О. Ю.; Головина, И. В.; Щербань, С. А.; Вялова, О. Ю.
Исследован характер распределения полихлорированных бифенилов (ПХБ) в тканях Mytilus galloprovincials Lam. в условиях хронического токсикологического эксперимента с применением ароклора 1254. У животных из естественных популяций (контрольная группа) данные химические соединения аккумулируются преимущественно в гепатопанкреасе и ноге. При содержании моллюсков в воде с концентрацией ароклора 1254 - 110 нг л-1 (экспозиция 21 день) существенный прирост уровня ПХБ отмечен в жабрах и ноге на 57 и 160 % (р&lt;0.05) соответственно. На этом фоне в данных тканевых структурах отмечали развитие гипоксии гистоток-сического типа. Индекс МДГ/ЛДГ вырос в 2.2 - 2.6 раза (р&lt;0.001). Одновременно происходило угнетение скорости процессов регенерации в жабрах. Индекс РНК/ДНК...
Tipo: Journal Contribution
Palavras-chave: Полихлорированные бифенилы; Mytilus galloprovincials; Малатдегидрогеназа; Лактатдегид-рогеназа; РНК; ДНК; Каротиноиды; Polychlorinated biphenyls; Mytilus galloprovincials Lam.; Malate dehydrogenase; Lactate dehydrogenase; RNA; DNA; Carotinoids.
Ano: 2005
URL: http://repository.ibss.org.ua/dspace/handle/99011/236

RNA na saúde e na agricultura.
Provedor de dados: 14
Autores: LOPES, M. A.
A possibilidade de produzir com rapidez vacinas seguras contra zoonoses ou doenças infecciosas poderá ter enorme impacto na saúde pública e na pecuária.
Tipo: Artigo de periódico
Palavras-chave: Doenças infecciosas; Vacina; Zoonose; RNA.
Ano: 2020
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1137928

Engenharia genética: conceitos básicos, ferramentas e aplicações.
Provedor de dados: 14
Autores: CORDEIRO, M. C. R.
ABSTRACT: Basic concepts and methodologies concerning genetic engineering are presented in this review and is directed to people or professionales not familiarized to it. Moreover, it can be a document to clarity what is genetic engineering, the importance of its contributions to modern agriculture, medicine or livestock research and to support discussions on genetic research ethics. Covers information on structure of nucleic acids, types, functions and main processes such us DNA duplication, transcription and traduction. Important methodologies are covered about DNA/RNA extraction, electrophoresis, PCR, nucleic acid libraries production, cloning, sequencing, gene characterization in connection to main historical events of genetic engineering. It...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Ácido Nucléico; Biologia Molecular; Biotecnologia; Clonagem; DNA; Engenharia Genética; Gene; Marcador Genético; RNA; Organismo Transgênico.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/568132

Development of genomic probes for detection of hepatitis A virus and enteroviruses in shellfish
Provedor de dados: 5
Autores: Apaire, M; Leguyader, F; Besse, B; Billaudel, S.
The development of molecular biology techniques has modified research procedures for certain viruses, particularly hepatitis A virus (HAV) and enteroviruses. Accordingly, we designed cDNA and RNA genomic probes to detect viruses in shellfish in the environment. Better sensitivity was noted with RNA than cDNA probes: only 17/83 shellfish samples were positive with HAV and enterovirus DNA probes, where as 42/83 were positive with HAV and enterovirus RNA probes.
Tipo: Text
Palavras-chave: Mollusca; Enterovirus; Hepatitis A virus; Picornaviridae; RNA probes; DNA probes; Seafood; Biology; Sensors; DNA; RNA; Viruses; Detection; Marine molluscs; Shellfish.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/1992/acte-1613.pdf

Liaison de l'oestradiol 17 beta dans les hepatocytes de truite Arc-en-ciel et induction de la vitellogenine et de son ARNm specifique
Provedor de dados: 5
Autores: Maitre, J; Mercier, L; Dolo, L; Valotaire, Y.
In the liver of rainbow trout (Salmo gairdnerii ), vitellogenin (Vg) synthesis is influenced by oestradiol (E sub(2)) which is believed to act through the classical mechanism of steroid hormone action. After binding of the hormone to a soluble specific receptor protein, the oestradiol-receptor complex is translocated to the nucleus, where it interacts with DNA and modulates expression of Vg genes, leading to increased synthesis of specific mRNA and Vg. The authors show here: (i) the presence of specific oestrogen receptors in the cytosol of the male trout liver. (ii) The male liver, offering, an ideal experimental control of "zero" background, the authors followed - in the liver of male trouts - the kinetics of induction of Vg mRNA by hybridization with Vg...
Tipo: Text
Palavras-chave: Salmonidae; Pisces; Proteins; Liver; RNA; Steroids; Biosynthesis; Receptors; Sex hormones; Sexual reproduction.
Ano: 1983
URL: http://archimer.ifremer.fr/doc/1983/acte-1230.pdf

The effect of nucleic acids on growth, ureagenesis and nitrogen excretion of gilthead sea bream Sparus aurata juveniles
Provedor de dados: 5
Autores: Oliva Teles, A; Guedes, M; Vachot, Christianne; Kaushik, Sadasivam.
The aim of this trial was to evaluate the effect of dietary nucleic acids on ureagenesis and nitrogen balance in gilthead sea bream juveniles. For that purpose, 5 isonitrogenous (7.25% N) diets were formulated based on fish meal as protein source (FM-control diet) and to partially replace the fish meal nitrogen with two levels of nucleic acids (diets RNA1 and RNA2) or brewers yeast (diets BY1 and BY2). Each diet was fed for 10 weeks, to apparent visual satiety, to triplicate groups of 50 fish each with an average body weight of 12.7 g.   Inclusion of nucleic acid N either as brewers yeast or RNA led to significant improvement of feed intake and growth, except for fish fed diet RNA2. There were no differences in final weight between groups fed the yeast-...
Tipo: Text
Palavras-chave: Sea bream; N accretion; Ureagenesis; Brewers yeast; RNA.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-6681.pdf

病原体媒介蚊が有するウイルスとの相互作用の分子基盤
Provedor de dados: 19
Autores: 嘉糠, 洋陸; KANUKA, HIROTAKA.
2008年～2010年科学研究費補助金若手研究(A)研究成果報告書20688013
Palavras-chave: 蚊; ウイルス; RNA; 干渉; 自然免疫; 感染症; 節足動物.
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3284

coordinated research on groundnut of Rosette virus disease
Provedor de dados: 30
Autores: D. McDonald.
Palavras-chave: Groundnuts; Viruses; RNA; Grafting; ELISA; Genotypes; Backcrossing; Breeds (animals); Resistance varieties; Scions.
Ano: 1987
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3144

Working together on groundnut virus diseases
Provedor de dados: 30
Autores: D.V.R.
Palavras-chave: Viruses; Groundnuts; RNA; Genes; ELISA; Genotypes; Transgenics; PCR; Genetic structures.
Ano: 1994
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4178

Groundnut Virus Diseases in Africa
Provedor de dados: 30
Autores: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT).
Palavras-chave: Groundnuts; Viruses; Genotypes; RNA; Quarantine; Breeds (animals); Genetic markers; Genes; Recessive genes; Resistance varieties.
Ano: 1991
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4093

Working Together on Groundnut Virus Diseases
Provedor de dados: 30
Autores: D.V.R.( editor).
Palavras-chave: Viruses; Groundnuts; RNA; Genes; ELISA; Genotypes; Transgenics; PCR; Genetic structures.
Ano: 1994
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3121

Transformation and Regeneration of Groundnut, and Utilization of Viral Genes to Induce Resistance to Virus Diseases
Provedor de dados: 30
Autores: D.V.R.
Palavras-chave: Genes; RNA; Transgenics; Protoplasts; Groundnuts; Transgenic plants; Biotechnology; Regeneration; Genomes; Nucleotides.
Ano: 1992
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4105

Coordinated Research on Groundnut Rosette Virus Disease
Provedor de dados: 30
Palavras-chave: Groundnuts; Viruses; RNA; Grafting; ELISA; Genotypes; Backcrossing; Breeds (animals); Resistance varieties; Scions.
Ano: 1988
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4054

Effects of cottonseed meal supplemented with lysine and enzyme (Hydroenzyme XP) on egg quality and performance of laying hens
Provedor de dados: 96
Autores: Saki,Ali Asghar; Pournia,Khashayar; Tabatabaie,Mohamad Mehdi; Zamani,Pouya; Haghighat,Mojtaba; Salary,Jalal.
This study was conducted to evaluate the effect of cottonseed meal supplemented with lysine and enzyme on laying hens performance and egg quality. Eighty Hy-Line W-36 white Leghorns were allotted for 12 weeks in a 2 × 2 factorial experiment in a completely randomized design, including four treatments and five replications with four birds in each. Treatments included: basal diet + 1% lysine + 0% enzyme; basal diet + 1% lysine + 0.025% enzyme; basal diet + 2% lysine + 0% enzyme; and basal diet + 2% lysine + 0.025% enzyme. Protein content in the magnum tissue was not significantly affected by different levels of lysine and enzyme, although magnum Protein:RNA ratio increased with 2% of lysine as compared with 1%. Moreover, jejunum DNA's concentration was not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cottonseed meal; DNA; Performance; Protein synthesis; RNA.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000013

Molecular detection of Potato yellow vein virus in the natural whitefly vector Trialeurodes vaporariorum, Westwood
Provedor de dados: 100
Autores: Barragan,Carlos Eduardo; Guzmán-Barney,Mónica.
Potato yellow vein virus (PYVV) expresses yellowing symptoms on plants and it is transmitted by the whitefly vector Trialeurodes vaporariorum Westwood. No molecular viral detection from the vector extracts has been reported to date. The objectives of this study were I) amplification of the PYVV major coat protein (CP) gene from viruliferous vector extracts by RT-PCR using specific primers, II) cloning and III) sequencing and sequences analysis. Some Colombian Solanum phureja (Jus et Buk) field potato plants expressing yellowing symptoms and confirmed PYVV positive by RT-PCR were used as virus donor plants for feeding whiteflies. Viruliferous vectors were obtained by depositing aviruliferous whiteflies on symptomatic leaves and kept in a muslin...
Tipo: Journal article
Palavras-chave: PYVV; RNA; Whitefly; Detection; Vector.
Ano: 2014
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522014000300002

LA DUALIDAD ANÁLOGO DIGITAL DE LA INFORMACIÓN SE EJEMPLIFICA EN EL ESTUDIO DE LAS MÓLECULAS DE RNA
Provedor de dados: 37
Autores: ANDRADE,EUGENIO.
Las investigaciones sobre las moléculas de RNA, han contribuido a precisar y formalizar la noción física de información considerada tanto en su aspecto analógico como digital. El dogma central de la biología molecular, restringe la noción de información al aspecto digital de la misma, y por tanto al copiado fiel de una cadena de símbolos en los procesos de replicación (DNA-DNA) y transcripción (DNA-RNA), e igualmente en la traducción a proteínas mediante el código genético. Esta visión genera la paradoja sobre qué surgió primero si DNA o proteínas, la cual se resuelve en parte recurriendo a la hipótesis según la cual existió un mundo prebiótico RNA, anterior a las proteínas codificadas genéticamente y al DNA. Esta hipótesis tiene soporte empírico sólido...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNA; DNA; Proteínas; Información digital; Información analógica; Dogma central; Mundo RNA.
Ano: 2011
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2011000300002

Campomanesia adamantium (Cambess.) O. Berg seed desiccation: influence on vigor and nucleic acids
Provedor de dados: 42
Autores: DRESCH,DAIANE M.; MASETTO,TATHIANA E.; SCALON,SILVANA P.Q.
The aim of this study was to evaluate the sensitivity of Campomanesia adamantium seeds to desiccation by drying in activated silica gel (fast) and under laboratory conditions (slow). To assess the sensitivity of the seeds to desiccation, we used drying with silica gel and drying under laboratory conditions (25 °C), in order to obtain seeds with moisture content of 45, 35, 30, 25, 20, 15, 10 and 5%. The physiological potential of the seeds after desiccation was evaluated by measuring primary root protrusion, percentage of normal seedlings, germination seed index, seedling length, total seedling dry mass, electrical conductivity and DNA and RNA integrities. The C. adamantium seeds were sensitive to desiccation and to a reduction in moisture content to 21.1%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerrado; DNA; Drying; RNA; Viability.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000502217

De novo transcriptome assembly, functional annotation and differential gene expression analysis of juvenile and adult E. fetida, a model oligochaete used in ecotoxicological studies
Provedor de dados: 48
Autores: Thunders,Michelle; Cavanagh,Jo; Li,Yinsheng.
Abstract  Background  Earthworms are sensitive to toxic chemicals present in the soil and so are useful indicator organisms for soil health. Eisenia fetida are commonly used in ecotoxicological studies; therefore the assembly of a baseline transcriptome is important for subsequent analyses exploring the impact of toxin exposure on genome wide gene expression.  Results  This paper reports on the de novo transcriptome assembly of E. fetida using Trinity, a freely available software tool. Trinotate was used to carry out functional annotation of the Trinity generated transcriptome file and the transdecoder generated peptide sequence file along with BLASTX, BLASTP and HMMER searches and were loaded into a Sqlite3 database. To identify differentially expressed...
Tipo: Journal article
Palavras-chave: Earthworm; Trinity; Transcriptome; E. fetida; Ecotoxicology; RNA; Sequence; Gene expression.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100401

Response of Paenibacillus polymyxa to iron: alternations in cellular chemical composition and the production of fusaricidin type antimicrobial compounds
Provedor de dados: 52
Autores: Raza,Waseem; Hongsheng,Wu; Qirong,Shen.
In this work, growth, cellular chemical composition and production of fusaricidin type antimicrobial compounds by P. polymyxa SQR-21 were compared in tryptone broth supplemented with four concentrations of iron (25, 50, 100 and 200 µM). The data revealed that the growth of P. polymyxa SQR-21 was increased by 3-8% with the increase in concentration of ferric ion (Fe3+). The production of fusaricidin type compounds was increased by 33-49% only up to 50 µM Fe3+ and the highest level of Fe3+ was inhibitory. Increase in the liquid culture Fe3+concentration increased the intracellular protein (2%), intracellular carbohydrate (14%), extracellular protein (7%) and polysaccharide contents (18%) while the intracellular lipid contents were increased (11%) only up to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemical composition; Fusaricidin; Iron; Paenibacillus polymyxa; RNA.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500019

Control of gene expression in Trypanosomatidae
Provedor de dados: 56
Autores: Teixeira,S.M.R.
The study of mechanisms which control gene expression in trypanosomatids has developed at an increasing rate since 1989 when the first successful DNA transfection experiments were reported. Using primarily Trypanosoma brucei as a model, several groups have begun to elucidate the basic control mechanisms and to define the cellular factors involved in mRNA transcription, processing and translation in these parasites. This review focuses on the most recent studies regarding a subset of genes that are expressed differentially during the life cycle of three groups of parasites. In addition to T. brucei, I will address studies on gene regulation in a few species of Leishmania and the results obtained by a much more limited group of laboratories studying gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma; Leishmania; Gene expression; RNA; Antigenic variation; VSG.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200001