Consulta: subjectFacets:"RT-PCR"
Registros recuperados: 100
Data/hora: 05/06/2024 10:32:17

Detección de transgenes en variedades nativas de maíz en dos regiones del estado de Puebla.
Provedor de dados: 32
Autores: Carreón Herrera, Nayelli Itzell.
La presencia de transgenes en variedades nativas de maíz mexicano ha sido motivo de controversia. Se sospecha una posible introducción a través de las tiendas rurales DICONSA (TRD). El objetivo de este estudio fue precisar si plantas provenientes del grano expendido por las TRD al sembrarse llegan a antesis, y si esta coincide con la floración femenina de los maíces nativos de municipios de la Mixteca Baja en el Estado de Puebla. También se buscó  cuantificar secuencias transgénicas, particularmente el promotor  del Virus  Mosaico de la coliflor (CaMV, por sus siglas en inglés) -35S en variedades nativas de maíz y en granos provenientes de TRD. Para el primer objetivo, en 2008 se establecieron dos experimentos en campo evaluando 46 poblaciones nativas y 23...
Palavras-chave: Maíz transgénico; Variedades nativas; Maíces nativos; Dinámica de floración; Promotor CaMV-35S; PCR Tiempo-Real; Flujo génico; Transgenic maize; Maize landraces; CaMV-35S promoter; RT-PCR; Gene flow; Flowering dynamics; Maestría; EDAR; Estrategias para el Desarrollo Agrícola Regional.
Ano: 2011
URL: http://hdl.handle.net/10521/439

Detección de transgenes en variedades nativas de maíz en dos regiones del estado de Puebla.
Provedor de dados: 32
Autores: Carreón Herrera, Nayelli Itzell.
La presencia de transgenes en variedades nativas de maíz mexicano ha sido motivo de controversia. Se sospecha una posible introducción a través de las tiendas rurales DICONSA (TRD). El objetivo de este estudio fue precisar si plantas provenientes del grano expendido por las TRD al sembrarse llegan a antesis, y si esta coincide con la floración femenina de los maíces nativos de municipios de la Mixteca Baja en el Estado de Puebla. También se buscó  cuantificar secuencias transgénicas, particularmente el promotor  del Virus  Mosaico de la coliflor (CaMV, por sus siglas en inglés) -35S en variedades nativas de maíz y en granos provenientes de TRD. Para el primer objetivo, en 2008 se establecieron dos experimentos en campo evaluando 46 poblaciones nativas y 23...
Palavras-chave: Maíz transgénico; Variedades nativas; Maíces nativos; Dinámica de floración; Promotor CaMV-35S; PCR Tiempo-Real; Flujo génico; Transgenic maize; Maize landraces; CaMV-35S promoter; RT-PCR; Gene flow; Flowering dynamics; Maestría; EDAR; Estrategias para el Desarrollo Agrícola Regional.
Ano: 2011
URL: http://hdl.handle.net/10521/439

Identificación de virus asociados a la nochebuena de sol (Euphorbia pulcherrima Willd. Ex Klotzch) en México.
Provedor de dados: 32
Autores: Ocampo Ocampo, Tania.
El Campo Experimental “Zacatepec”, del INIFAP, inició un programa de mejoramiento  genético de plantas silvestres y semicultivadas de nochebuena (“nochebuena de sol”). Dicho programa requiere de la generación de una amplia base de datos fitosanitarios que permitan la liberación permanente de nuevas variedades e híbridos con tolerancia a las principales enfermedades. En México no hay con información sobre presencia de virus en nochebuena de sol, por lo que el objetivo de la presente investigación fue generar información de los virus asociados a esta planta. La identificación de estos patógenos se realizó mediante pruebas ELISA, inmunotiras y RT-PCR; adicionalmente, se inocularon plantas indicadoras y se hizo microscopia electrónica de transmisión. Con...
Palavras-chave: DAS-ELISA; RT-PCR; TMV; PnMV; Nochebuena de sol; Wild poinsettia; Fitopatología; Maestría.
Ano: 2012
URL: http://hdl.handle.net/10521/1732

Efficient protocol for isolation of functional RNA from different grape tissue rich in polyphenols and polysaccharides for gene expression studies
Provedor de dados: 69
Autores: Vasanthaiah,Hemanth K.N.; Katam,Ramesh; Sheikh,Mehboob B.
Extraction of RNA from plant tissue containing high levels of polyphenols and polysaccharides is tedious and difficult in grapes. Although several protocols have been published for plant RNA isolation, most have failed to yield high quality RNA in sufficient quantity from mature and diseased grape tissue. We describe a protocol for isolating intact and high quality RNA from various grape tissues as evident by high A260/A280 absorbance ratio (1.8 to 1.9) and electrophoretic profile on denaturing formaldehyde agarose gel. On an average, 205 µg RNA per g of fresh tissue were obtained using this modified protocol. RNA quality was further assessed through RT-PCR, differential display RT-PCR and subtractive hybridization, and found to be suitable for molecular...
Tipo: Journal article
Palavras-chave: Differential display RT-PCR; Grape; RNA isolation; RT-PCR; Subtractive hybridization; Vitis.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300005

Comparison of different methods for total RNA extraction from sclerotia of Rhizoctonia solani
Provedor de dados: 69
Autores: Shu,Canwei; Sun,Si; Chen,Jieling; Chen,Jianyi; Zhou,Erxun.
Background Rhizoctonia solani (teleomorph: Thanatephorus cucumeris) is one of the most important pathogens of rice (Oryza sativa L.) that causes severe yield losses in all rice-growing regions. Sclerotia, formed from the aggregation of hyphae, are important structures in the life cycles of R. solani and contain a large quantity of polysaccharides, lipids, proteins and pigments. In order to extract high-quality total RNA from the sclerotia of R. solani, five methods, including E.Z.N.A.&amp;#8482; Fungal RNA Kit, sodium dodecyl sulfate (SDS)-sodium borate, SDS-polyvinylpyrrolidone (PVP), guanidinium thiocyanate (GTC) and modified Trizol, were compared in this study. Results The electrophoresis results showed that it failed to extract total RNA from the...
Tipo: Journal article
Palavras-chave: Polyvinylpyrrolidone; Rhizoctonia solani; RT-PCR; Sclerotia; Sodium dodecyl sulfate; Total RNA extraction.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100009

Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
Provedor de dados: 74
Autores: Paiva,Marcelo Henrique Santos; Guedes,Duschinka Ribeiro Duarte; Leal,Walter Soares; Ayres,Constância Flávia Junqueira.
Abstract Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (&lt; 106 RNA copies/ml).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ZIKV; RT-PCR; Aedes aegypti; Vector competence.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400597

Frequencies of BCR-ABL1 fusion transcripts among Sudanese chronic myeloid leukaemia patients
Provedor de dados: 74
Autores: Osman,Emad-Aldin I.; Hamad,Kamal; Elmula,Imad M. Fadl; Ibrahim,Muntaser E.
The incidence of one or other rearrangement in chronic myeloid leukemia (CML) patients varies in different reported series. In this study we report the frequencies of BCR-ABL1 fusion transcript variants studied in 43 CML patients from Sudan. The study includes 46 Sudanese patients, three of which negative for the BCR-ABL1 fusion transcript. More than half of 43 positive patients showed b2a2 fusion transcript (53.5%), while (41.9%) showed b3a2 transcript and the remaining (4.6%) coexpression of b3a2/ b2a2 and b3a2/b2a2/e19a2. We detected neither coexpression of p210/p190 nor e1a2 alone. Male patients showed a tendency to express b2a2, while female tende to express b3a2 (p = 0.017). Moreover, a single nucleotide polymorphism was detected in BCR exon 13 in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BCR-ABL; Chronic myeloid leukemia; Ph chromosome; Sudanese; RT-PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200005

Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis
Provedor de dados: 74
Autores: Almeida-Val,Vera Maria Fonseca; Oliveira,Alice Reis; Silva,Maria de Nazaré Paula da; Ferreira-Nozawa,Monica Stropa; Araújo,Roziete Mendes; Val,Adalberto Luis; Nozawa,Sérgio Ricardo.
Adaptation or acclimation to hypoxia occurs via the modulation of physiologically relevant genes, such as erythropoietin, transferrin, vascular endothelial growth factor, phosphofructokinase and lactate dehydrogenase A. In the present study, we have cloned, sequenced and examined the modulation of the LDH-A gene after an Amazonian fish species, Astronotus crassipinis (the Oscar), was exposed to hypoxia and anoxia. In earlier studies, we have discovered that adults of this species are extremely tolerant to hypoxia and anoxia, while the juveniles are less tolerant. Exposure of juveniles to acute hypoxia and anoxia resulted in increased LDH-A gene expression in skeletal and cardiac muscles. When exposed to graded hypoxia juveniles show decreased LDH-A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazon; Fishes; Gene expression; RT-PCR; LDH-A; Hypoxia; Anoxia.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200025

Development of a quantitative competitive reverse transcriptase polymerase chain reaction for the quantification of growth hormone gene expression in pigs
Provedor de dados: 74
Autores: Franco,Maurício Machaim; Almeida,Juliana Franco; Souza,Guilherme Rocha Lino de; Antunes,Robson Carlos; Goulart,Luiz Ricardo.
After the advent of the genome projects, followed by the discovery of DNA polymorphisms, basic understanding of gene expression is the next focus to explain the association between polymorphisms and the level of gene expression, as well as to demonstrate the interaction among genes. Among the various techniques for the investigation of transcriptional profiling involving patterns of gene expression, quantitative PCR is the simplest analytical laboratory technique. The objective of this work was to analyze two strategies of a competitive PCR technique for the quantification of the pig growth hormone (GH) gene expression. A pair of primers was designed targeting exons 3 and 5, and two competitive PCR strategies were performed, one utilizing a specific...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pig; GH gene; Gene expression; RT-PCR.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100003

Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements
Provedor de dados: 74
Autores: Liang,Qiaoyi; Ding,Jiayi; Zheng,Shu.
Up-regulation of human endogenous retroviruses (HERVs) is associated with many diseases, including cancer. In this study, an H family HERV (HERV-H)-related gene was identified and characterized. Its spliced transcript lacks protein-coding capacity and may belong to the emerging class of noncoding RNAs (ncRNAs). The 1.3-kb RNA consisting of four exons is transcribed from an Alu element upstream of a 5.0-kb structurally incomplete HERV-H element. RT-PCR and quantitative RT-PCR results indicated that expression of this HERV-related transcript was negatively associated with colon, stomach, and kidney cancers. Its expression was induced upon treatment with DNA methylation and histone deacetylation inhibitors. A BLAT search using long terminal repeats (LTRs)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endogenous retrovirus; Noncoding RNA; RT-PCR; Quantitative real-time PCR; Transcription.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400005

In silicio expression analysis of PKS genes isolated from Cannabis sativa L.
Provedor de dados: 74
Autores: Flores-Sanchez,Isvett J.; Linthorst,Huub J.M.; Verpoorte,Robert.
Cannabinoids, flavonoids, and stilbenoids have been identified in the annual dioecious plant Cannabis sativa L. Of these, the cannabinoids are the best known group of this plant's natural products. Polyketide synthases (PKSs) are responsible for the biosynthesis of diverse secondary metabolites, including flavonoids and stilbenoids. Biosynthetically, the cannabinoids are polyketide substituted with terpenoid moiety. Using an RT-PCR homology search, PKS cDNAs were isolated from cannabis plants. The deduced amino acid sequences showed 51%-73% identity to other CHS/STS type sequences of the PKS family. Further, phylogenetic analysis revealed that these PKS cDNAs grouped with other non-chalcone-producing PKSs. Homology modeling analysis of these cannabis PKSs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cannabis sativa; Homology modeling; Polyketide synthases; RT-PCR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400017

Crude alkaloid extract of Rhazya stricta inhibits cell growth and sensitizes human lung cancer cells to cisplatin through induction of apoptosis
Provedor de dados: 74
Autores: Elkady,Ayman I.
There is an urgent need to improve the clinical management of non-small cell lung cancer (NSCLC), one of the most frequent causes of cancer-related deaths in men and women worldwide. Rhazya stricta, an important medicinal plant used in traditional Oriental medicine, possesses anti-oxidant, anti-carcinogenic and free radical scavenging properties. This study was done to explore the potential anticancer activity of a crude alkaloid extract of R. stricta (CAERS) against the NSCLC line A549. CAERS markedly suppressed the growth of A549 cells and considerably enhanced the anti-proliferative potential of cisplatin. CAERS-mediated inhibition of A549 cell growth correlated with the induction of apoptosis that was accompanied by numerous morphological changes, DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Lung cancer; Medicinal plant; RT-PCR; Western blot.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100003

Considerações estatísticas na análise de dados de expressão gênica gerados pela técnica de RT-qPCR.
Provedor de dados: 14
Autores: LOBO, A. M. B. O.; LOBO, R. N. B.
A transcriptase reversa (RT) seguida de quantificação utilizando a técnica da reação em cadeia da polimerase (qPCR) em tempo real ou, simplesmente reverse transcription-qPCR (RT-qPCR), é um método in vitro para amplificação enzimática de sequências definidas de DNA complementar obtido a partir do RNA. A técnica de RT-qPCR é uma das mais utilizadas em experimentos de quantificação da expressão gênica por ser considerado um dos métodos mais sensível e específico de quantificação gênica dos que outros utilizados para esta finalidade. No entanto, para obter resultados, pela técnica de RT-qPCR, que possam ser adequadamente analisados é necessário que o experimento tenha um desenho experimental com tratamentos, ensaios, unidade experimental e delineamento bem...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: RT-qPCR; Expressão gênica; Expressão gênica relativa; RT-PCR; Genetic processes.; Genética animal; Genética molecular; Gene expression; Genetics.
Ano: 2014
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1007398

Diagnóstico de mastite subclínica caprina por meio da detecção de Staphylococcus aureus utilizando a técnica de RT-PCR.
Provedor de dados: 14
Autores: MONTEIRO, J. P.; SOUZA, V. de.
A presente publicação descreve os procedimentos necessários para a identificação e confirmação molecular de estirpes de S. aureus causadoras de mastite subclínica, provenientes de amostras de leite de cabra, por meio da técnica de RT-PCR.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: RT-PCR; PCR.; Leite de cabra; Caprino; Teste de mamite; Staphylococcus aureus; Diagnóstico; Infecção; Doença animal; Goat milk; Mastitis; Diagnostic techniques; Animal diseases.
Ano: 2015
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1047787

Les Toxi-Infections Alimentaires Collectives (TIAC) virales associées à la consommation d'huîtres en France, hiver 2000-2001
Provedor de dados: 5
Autores: Zidane, Mohamed; Le Guyader, Soizick; Pommepuy, Monique.
During the winter 2000/2001, several outbreaks associated with the consumption of shellfish were declared in France. Following these events, a close cooperation was organized between administrations and laboratories (DGAL, DPMA, InVS, AFSSA, Ifremer, CHU-Dijon and CNC) to gather competences and to manage the situation. This report synthesizes the information collected during these outbreaks.  From the outset, the epidemiologic investigations directed the research towards viral contamination and shellfish as contamination source. Several outbreaks were investigated. Oysters and stools samples from ill patients were analysed by RT-PCR for viruses (enterovirus, rotavirus, norovirus and astrovirus). The genic amplification was done by using the same primers in...
Tipo: Text
Palavras-chave: Épidémie; Gastro-entérites; Huîtres; Contamination virale; RT-PCR; Norovirus; TIAC.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/00068/17941/15476.pdf

Differential gene expression in Litopenaeus vannamei shrimp in response to diet changes
Provedor de dados: 5
Autores: Chavez-calvillo, G.; Perez-rueda, E.; Lizama, G.; Zuniga Aguilar, J. J.; Gaxiola, G.; Cuzon, Gerard; Arena-ortiz, L.
We evaluated changes in gene expression of Litopenaeus vannamei shrimp in response to a change in diet by comparing specimens which were fed with an animal protein diet against those which underwent a change from an animal protein diet to a plant protein diet. In addition, we also compared specimens which were fed with a plant protein diet with another group which underwent a change from plant to animal protein diet. Results showed that, in the case of hepatopancreas tissue, a total of six genes were differentially expressed and exhibited a high degree of homology with sequences which codify for proteins related to metabolism, transcription regulation, and rRNA subunits. In the case of muscle tissue, four differentially expressed sequences were recorded...
Tipo: Text
Palavras-chave: Differential display; RT-PCR; Litopenaeus vannamei; Protein diet; Alternative protein sources.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00002/11298/7853.pdf

Genotoxicity and activation of cellular defenses in transplanted zebra mussels Dreissena polymorpha along the Seine river
Provedor de dados: 5
Autores: Chatel, Annelle; Faucet-marquis, Virginie; Gourlay-france, Catherine; Pfohl-leszkowicz, Annie; Hubert, Francoise.
The aim of the present study was to confirm the relevance of studying DNA adduct formation in a field study. In that context, freshwater mussels Dreissena polymorpha, collected in a reference station, were transplanted in different sites with a pollution gradient. After one and two months, mussels were collected and DNA adduct formation was analyzed using the 32P post labelling technique on both gills and digestive glands. In addition, the expression of genes involved in the detoxification system (catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), HSP70, aryl hydrocarbon receptor (AHR), P glycoprotein (PgP), metallothionein (MT)) was assessed by RT-PCR. DNA adducts were observed at amount comparable to data from literature....
Tipo: Text
Palavras-chave: Biomarker; Pollution; DNA adducts; Detoxification; Dreissena polymorpha; RT-PCR.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00195/30639/32450.pdf

Contamination de l'environnement littoral par les rotavirus du groupe A. Détection par amplification enzymatique en chaîne et analyse du polymorphisme de restriction de séquences virales dans les eaux de surfaces et les coquillages
Provedor de dados: 5
Autores: Dubois, Eric.
Les rotavirus font partie des principaux virus entériques humains susceptibles d'être rencontrés dans l'environnement. Leur mise en évidence par culture cellulaire dans des prélèvements est longue et peu sensible, ce qui explique le manque de données sur la contamination du littoral. La RT-seminested PCR a été développée pour pallier ces inconvénients. Après une mise au point méthodologique cette technique a été utilisée lors d'études in vitro et in situ. Les études in vitro sur le devenir des rotavirus en eau de mer, suivi par RT-PCR quantitative et par multiplication en culture cellulaire, montrent que dans des conditions proches de celles du milieu naturel (eau de mer non stérile et à une température relativement basse), la détection d'ARN viral peut...
Tipo: Text
Palavras-chave: Rotavirus; Contamination; Coquillages; Eaux de stations d'épuration; Echantillons cliniques; RT-PCR; RFLP; Epidémiologie moléculaire.
Ano: 1992
URL: http://archimer.ifremer.fr/doc/00442/55376/56894.pdf

TRANSCRIPTOS DE FUSIÓN DEL GEN BCR/ABL EN PACIENTES CON LEUCEMIA MIELOIDE CRÓNICA
Provedor de dados: 77
Autores: Artigas,Carmen Gloria; Melo,Angélica; Roa,Juan Carlos; Roa,Iván; Quijada,Ingrid; Vittini,Cecilia; Cabrera,María Elena; Risueño,Concepción.
La anormalidad citogenética más común en la leucemia mieloide crónica (LMC) es el cromosoma Philadelphia, producida por la t(9;22), cuya expresión molecular es el gen de fusión BCR-ABL, que codifica proteínas con actividad tirosinquinasa. Según el punto de ruptura de los genes BCR o ABL se produce una proteína de fusión de 210-kD(p210) o 190-kD(p190). La presencia de este gen de fusión en pacientes con LMC tiene implicancia diagnóstica. Con el propósito de detectar transcriptos de fusión del gen BCR/ABL en pacientes con leucemia mieloide crónica, procedentes de la IX Región de Chile, se estudiaron 14 muestras de sangre de 11 pacientes con LMC. A 2 de ellos, se les realizó seguimiento durante su tratamiento con Gleevec. Se aplicó la técnica de reacción en...
Tipo: Journal article
Palavras-chave: BCR/ABL; RT-PCR; Leucemia mieloide crónica; Chile.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000300004

Virus de Distemper Canino: detección molecular de diferentes aislamientos provenientes de perros de la provincia de santa fe, argentina, entre los años 2000 y 2010
Provedor de dados: 78
Autores: Pinotti,M.; Gollan,A; Canavesio,M.; Passeggi,C.; Larrateguy,M.V.; Paz,M.E.; Formentini,E.
En la ciudad de Santa Fe, Argentina, durante los años 2000 y 2010, se aislaron 13 cepas autóctonas del virus Distemper Canino. Las muestras se tomaron de caninos con signos clínicos de la enfermedad y consistieron en hisopados nasales, oculares, faríngeos (tonsilas) y conjuntivales. Los aislamientos se realizaron en células MDCK, las cuales mostraron efecto citopatogénico característico. Los virus provenientes de la cosecha, se detectaron por RT-PCR de una región que codifica la nucleoproteína NP asociada al RNA (posición 769 a la 1055 del genoma) obteniéndose en todos los casos un producto de 287 pb comparable con el obtenido con cepas vacunales (cepas Ondersterpoort y Lederle), lo que indica presencia viral en todas las muestras procesadas. Las cepas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Virus; Distemper canino; Estudio molecula; RT-PCR.
Ano: 2016
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982016000200007

Rabies virus in a pregnant naturally infected southern yellow bat (Lasiurus ega)
Provedor de dados: 80
Autores: Allendorf,SD; Albas,A; Cipriano,JRB; Antunes,JMAP; Appolinário,CM; Peres,MG; da Rosa,AR; Sodré,MM; Megid,J.
Current knowledge on bat lyssavirus infections in their native hosts is limited and little is known about the virulence, virus dissemination and transmission among free-living insectivorous bats. The present study is a brief description of rabies virus (RABV) dissemination in tissues of a naturally infected pregnant southern yellow bat (Lasiurus ega) and its fetuses, obtained by reverse-transcriptase polymerase chain reaction (RT-PCR). The RT-PCR was positive in samples from the brain, salivary gland, tongue, lungs, heart, kidneys and liver. On the other hand, the placenta, three fetuses, spleen, intestine and brown fat tissue tested negative. This research demonstrated the absence of rabies virus in the fetuses, thus, in this specific case, the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rabies; Bats; Vertical transmission; RT-PCR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200014

Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART)
Provedor de dados: 80
Autores: Meira,DA; Almeida,RAMB; Barbosa,AN; de Souza,LR; Olivo,TET; Henriques,RMS; Golim,MA; Araújo Jr,JP; Nagoshi,LR; Orikaza,CM; Calvi,SA.
A cross-sectional study was performed on HIV-1 infected individuals with or without antiretroviral treatment (ARV) in the AIDS Day Hospital, Botucatu Medical School, UNESP. Between August 2004 and October 2005, 73 HIV-1 infected individuals were divided into three groups: infected individuals with or without AIDS who had never received ARV (G1 = 15); patients on HAART that had had plasma HIV-1 RNA viral load (VL) equal to or greater than 50 copies/mL (G2 = 27); and patients on HAART with undetectable VL for at least the past six months (G3 = 31). There was also an additional group that comprised blood donors without any sign of the disease and with negative HIV serum tests (G4 = 20), which was the control group. Serum cytokine levels (values in pg/mL) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RT-PCR; ELISA; Cytokines; HIV; AIDS; HAART; Apoptosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400011

Detection of three Allexivirus species infecting garlic in Brazil
Provedor de dados: 86
Autores: Melo Filho,Péricles de Albuquerque; Nagata,Tatsuya; Dusi,André Nepomuceno; Buso,José Amauri; Torres,Antonio Carlos; Eiras,Marcelo; Resende,Renato de Oliveira.
Garlic viruses often occur in mixed infections under field conditions. In this study, garlic samples collected in three geographical areas of Brazil were tested by Dot-ELISA for the detection of allexiviruses using monoclonal specific antibodies to detect Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C) and a polyclonal antiserum able to detect the three virus species mentioned plus Garlic virus D (GarV-D). The detected viruses were biologically isolated by successive passages through Chenopodium quinoa. Reverse Transcriptase Polimerase Chain Reaction (RT-PCR) was performed using primers designed from specific regions of the coat protein genes of Japanese allexiviruses available in the Genetic Bank of National Center of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium sativum; Virus detection; Coat protein; Dot-ELISA; RT-PCR.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000800002

Variabilidade genética de Sugarcane mosaic virus, causando mosaico em milho no Brasil
Provedor de dados: 86
Autores: Gonçalves,Marcos Cesar; Galdeano,Diogo Manzano; Maia,Ivan de Godoy; Chagas,César Martins.
O objetivo deste trabalho foi caracterizar biológica e molecularmente três isolados de Sugarcane mosaic virus (SCMV) de lavouras de milho, analisá-los filogeneticamente e discriminar polimorfismos do genoma. Plantas com sintomas de mosaico e nanismo foram coletadas em lavouras de milho, no Estado de São Paulo e no Município de Rio Verde, GO, e seus extratos foliares foram inoculados em plantas indicadoras e submetidos à análise sorológica com antissoros contra o SCMV, contra o Maize dwarf mosaic virus (MDMV) e contra o Johnsongrass mosaic virus (JGMV). Mudas de sorgo 'Rio' e 'TX 2786' apresentaram sintomas de mosaico após a inoculação dos três isolados, e o DAS-ELISA confirmou a infecção pelo SCMV. O RNA total foi extraído e usado para amplificação por...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Potyviridae; Zea mays; Mosaico comum do milho; RFLP; RT-PCR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000400004

Primer reporte de Tobacco mosaic virus (TMV) y Poinsettia mosaic virus (PnMV) en nochebuena de sol (Euphorbia pulcherrima Willd. Ex Klotzch) en México
Provedor de dados: 85
Autores: Ocampo Ocampo,T; Ochoa Martínez,DL; Ramírez Rojas,S; Valdovinos Ponce,G; Nava Díaz,C.
El Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), a través del Campo Experimental "Zacatepec" en el estado Morelos, inició un programa de mejoramiento genético de plantas silvestres y semicultivadas de nochebuena ("nochebuena de sol"). Dicho programa requiere de la generación de una base de datos fitosanitarios con tolerancia a los principales patógenos. México no cuenta con información sobre las enfermedades causadas por virus en la nochebuena de sol, por lo que el objetivo de la presente investigación fue generar datos preliminares sobre los virus asociados a estos materiales. Con base en los resultados obtenidos en las técnicas DAS-ELISA y RT-PCR, se reportan por primera vez en México los virus Tobacco mosaic virus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nochebuena silvestre; Nochebuena semicultivada; Virus; DAS-ELISA; RT-PCR.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572013000200011

Modulation of Physiologic Parameters in Molted Isa Brown Laying-Hens by Glutamine + Glutamic Acid Supplementation
Provedor de dados: 89
Autores: Morales,W; Rodríguez,V; Garcia,NV.
ABSTRACT Feed costs are the main limiting factors in poultry industry and alternative sources of food and/or feed supplements to optimize the bird´s life cycle and to extend their production period need to be explored. This study evaluated morphometric parameters of the small intestine and gonadotropin transcript levels in Isa Brown laying hens supplemented with glutamine + glutamic acid (Aminogut®) during a second production cycle. Molting was induced and groups of 100 hens each, were supplemented with 0, 0.8, 1.6 or 2.4% Aminogut® in their diet. At the end of the experimental period, tissue sections from duodenum, jejunum and ileum were processed by the Hematoxylin-Eosin technique and samples from hypothalamus and hypophysis were collected for RT-PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amino acids; Gonadotropin; Morphometry; RT-PCR; Small intestine.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000200255

Correlation Analysis of Relative Expression of Apob, Adfp and Fatp1 with Lipid Metabolism in Daweishan Mini Chickens
Provedor de dados: 89
Autores: Decai,X; Zhiyong,Z; Bin,Z; Zhongcheng,H; Quanshu,W; Jing,L.
ABSTRACT Quantitative RT-PCR was applied to measure the relative expression levels of the adipose differentiation-related protein (ADFP) gene, fatty acid transport protein 1 (FATP1) gene and apolipoprotein B (ApoB) gene in subcutaneous fat, abdominal fat, liver and muscle at five growth stages (28, 49, 70, 91 and 112 d) to determine the effect of the expression ofthese genes on fat deposition in Daweishan Mini chickens.The relative expression of ADFP gene mRNA in the abdominal fat andthe liver was significantly different between 49 d and 70 d (p&lt;0.05). The relative ApoB gene expression on 91d was higher in the liver, followed by muscles,subcutaneous fat, and abdominal fat, and was significantly higher in the liver than in the other three tissues. FATP1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ADFP; FATP1; ApoB; Daweishan Mini chicken; Fat traits; RT-PCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000100151

Investigation of Influenza A, West Nile and Newcastle Disease Viruses in Birds from the Pantanal Wetlands of Mato Grosso, Brazil
Provedor de dados: 89
Autores: Pinto,LB; Ometto,T; Araújo,J; Thomazelli,LM; Seixas,MM; Barbosa,CM; Ramos,DGS; Melo,ALT; Pinho,JB; Durigon,EL; Aguiar,DM.
ABSTRACT The Pantanal is the world's largest wetland biome with a seasonal flood pulse that attracts a great diversity of birds, many of which are migratory. Birds can be natural reservoirs Influenza A, West Nile and Newcastle Disease viruses. However, the occurrence of carriers for these viruses in the Pantanal was not verified yet. The present study evaluated the occurrence of natural infection by Influenza A, WN and ND virus of birds in the municipality of Poconé, a subregion of the Pantanal in the state of Mato Grosso, Brazil. A total of 76 birds belonging to 11 orders and 20 families were captured using mist nets. The most representative order was Passeriformes, followed by the other nine orders, which included Columbiformes, Psittaciformes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pantanal birds; Infectious Diseases; RT-PCR; QRT-PCR; Virus.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200291

Effect of maternally-derived antibodies on the performance and immunity of broilers induced by in ovo or post-hatching immunizations with a live vaccine against infectious bursal disease
Provedor de dados: 89
Autores: Michell,BC; Gomes,AD; Baião,NC; Resende,M; Lara,LJC; Martins,NRS.
The interference of low or high maternal antibodies titers on the attenuated infectious bursal disease (IBD) virus (IBDV) vaccine infection and its effects on the performance of broilers vaccinated at the 18th day of incubation (in ovo), at one day of age (subcutaneously-SC), or at 15 days of age (drinking water-DW) were investigated. After a series of three live vaccinations, breeders were given or not an IBD oil emulsion vaccine (IBD-OEV) prior to sexual maturity. At day 18 of incubation (in ovo), a commercial vaccine containing HVT and an intermediate IBDV strain or the single HVT vaccine was given. An intermediate IBDV vaccine was given SC at one day of age, or at 15 days of age via DW. The progeny of unvaccinated breeders presented higher neutralizing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Attenuated IBDV; Attenuated IBDV neutralization; Broiler chicken; Infectious bursal disease; Infectious bursal disease virus; IBDV; In-ovo vaccination; Maternal antibody; RT-PCR; Serum neutralization; Vaccination versus performance.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000100009

Padronização da metodologia do RT-PCR utilizado para identificação do mRNA da alfa-amilase em sementes de milho
Provedor de dados: 95
Autores: Dantas,Bárbara França; Aragão,Carlos Alberto; Araújo-Junior,João Pessoa; Rodrigues,João Domingos; Cavariani,Cláudio; Nakagawa,João.
Durante a germinação das sementes, os carboidratos de reserva são degradados pela atividade de a-amilase. A identificação de mRNA é uma ferramenta fundamental para a definição da cinética de síntese de alfa-amilase. Objetivou-se padronizar a metodologia do RT-PCR para identificar o mRNA do gene de a-amilase em sementes de milho. Após três dias de germinação das cultivares Saracura-BRS 4154 e CATI-AL34, extraiu-se o RNA total pelo método do tiocianato de guanidina-fenol-clorofórmio, com algumas modificações. A partir do RNA total extraído foi obtido cDNA com utilização de "random primers". A amplificação por PCR de uma porção do gene da alfa-amilase foi realizada com os "primers": "sense" - CGACATCGACCACCTCAAC; "antisense" - TTGACCAGCTCCTGCCTGTC; gelatina;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays; Sementes; Alfa-amilase; RT-PCR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222002000200019

Molecular cloning and expression patterns of the Vasa gene from Rana nigromaculata (Amphibia: Anura)
Provedor de dados: 103
Autores: Jia,Rui; Nie,Liu-Wang; Wang,Ning; Wang,Jingjing.
The Vasa protein is a member of the DEAD (Asp-Glu-Alu-Asp) box family of ATP-dependent RNA helicases. The Vasa gene is specifically expressed in germ-line cells of many metazoans and is known to play a critical role in gametogenesis and reproductive regulation. In this paper, we isolate the full length cDNA sequence of the Vasa gene from the frog Rana nigromaculata Hallowell, 1861. The open reading frame (ORF) encoding 398 amino acid residues has nine conserved motifs. According to the similarities at the amino acid sequenceythe phylogenetic analysis of Vasa gene was consistent with the evolution relationships from chordates to mammals. Furthermore, the expression pattern analysis of RnVasa mRNA, using the technique of Reverse Transcriptase-Polymerase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DEAD-box; Phylogenetic analysis; RT-PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702009000200014

Feed additives can differentially modulate NF-κB (RelA/p65), IGF-1, GLUT2, and SGLT1 gene expression in porcine jejunal explants
Provedor de dados: 96
Autores: Silveira,Hebert; Melo,Antonio Diego Brandão; Bortoluzzi,Cristiano; Costa,Leandro Batista; Rostagno,Marcos Horácio; Schinckel,Allan Paul; Garbossa,Cesar Augusto Pospissil; Cantarelli,Vinícius de Souza.
ABSTRACT The intestinal gene expression of RelA/p65 (NF-κB), insulin growth factor 1 (IGF-1), glucose transporter 2 (GLUT2), and Na+/dependent glucose transporter 1 (SGLT1) were evaluated in response to benzoic acid, yeast culture, L-glutamine, and oregano essential oil, using an ex vivo model. Six piglets weighing approximately 20 kg each were sacrificed, and their jejunum was collected and segmented into five 2-cm explants. Each explant was immersed in cell culture medium according to one of the following treatments: control (without additive), 0.5% benzoic acid, 1% yeast culture, 1% L-glutamine, and 0.015% oregano oil. Gene expression was evaluated using RT-PCR. Yeast culture up-regulated the gene expression of RelA/p65, IGF-1, GLUT2, and SGLT1 in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemosensors; Glucose; Piglet; RT-PCR; Swine; Trophic effects.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100414

Efecto del virus del síndrome reproductivo y respiratorio porcino (PRRS) en células dendríticas de cerdo derivadas de monocitos
Provedor de dados: 108
Autores: Flores-Mendoza,Lilian; Silva-Campa,Erika; Reséndiz,Mónica; Mata-Haro,Verónica; Osorio,Fernando A.; Hernández,Jesús.
Las células dendríticas (DC) son las presentadoras de antígeno más importantes del sistema inmune. Su localización anatómica (piel, mucosas y sangre periférica), la expresión de receptores para reconocer patógenos, la expresión de moléculas de coestimulación (CD80/86), del complejo principal de histocompatibilidad (MHC) clases I y II, y la producción de citocinas (IFN-α, IL-10, IL-12), les confiere una característica única para regular las respuestas inmune innata y adaptativa. El objetivo de este trabajo fue evaluar el efecto del virus de síndrome reproductivo y respiratorio porcino (PRRS) en DC maduras. Se generaron células dendríticas a partir de monocitos utilizando IL-4 y GM-CSF y se estimularon con lipopolisacárido (LPS) para inducir su maduración....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Células dendríticas; Cerdo; CD80; CD86; MHC-II; RT-PCR.
Ano: 2009
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922009000100005

Secuenciación de un fragmento de ADNc homólogo a interleucina-1 alfa humana derivado de leucocitos de armadillo (Dasypus novemcinctus)
Provedor de dados: 108
Autores: Flores-Medina,Saúl; Díaz-García,Francisco J.; Guerra-Infante,Fernando M.
Se realizó detección del ARN mensajero (ARNm) mediante un ensayo de transcripción reversa acoplada a la reacción en cadena de la polimerasa (RT-PCR) específico para la detección de Interleucina-1 alfa humana en leucocitos de armadillo estimulados con acetato de forbol miristato (PMA, por su siglas en inglés); esta estrategia permitió amplificar un fragmento de ADN de 491 pares de bases. Adicionalmente, la secuencia mostró alta homología nucleotídica con las secuencias de ADNc humano (99%), mono (93%), cerdo (82%), caballo (81%) y llama (81%), mientras que la secuencia deducida de aminoácidos fue compatible con el precursor de la proteína IL-1a humana. Estos resultados sugieren presencia del gen de interleucina-1 en el genoma del armadillo; sin embargo, es...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Dasypus novemcinctus; Interleucina-1; RT-PCR; Secuenciación.
Ano: 2008
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922008000300008

Occurrence of congenital in Theileria equi Lusitano pure blood foals, diagnosed by RT-PCR
Provedor de dados: 195
Autores: Roncati, Neimar V.; Baccarin, Raquel Yvonne Arantes; Massoco, Cristina de Oliveira; Fernandes, Wilson Roberto.
In order to determine the occurrence of transplacentary transmission of Theileria equi in equine neonates, 50 foals of the Lusitano breed, both colts and fillies, along with their respective mothers were evaluated shortly after parturition. Total blood samples were collected from the mothers and the neonates within the first five hours after parturition for the detection of Theileria equi, through use of the RT-PCR technique. The detection kit based on the intercalating dye of DNA SYBERgreen was used. A total of 46% of the mares presented a positive result for Theileria equi and 54% presented negative ones, while 66% of the foals presented positive results, seeing as 73.9% of them were born from also positive mothers. The remaining 34% of foals presented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Theileria equi; Babesia equi; Babesiosis; RT-PCR; Transplacentary transmission; Congenital Theileria equi; Bacteria equi; Babesiose; RT-PCR; Transmissão transplacentária; Congênita.
Ano: 2011
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/386

Canine distemper diagnosis by RT-PCR in dog samples from São Paulo State submitted to rabies laboratory diagnosis
Provedor de dados: 195
Autores: Macedo, Carla Isabel; Peixoto, Zélia Maria Pinheiro; Castilho, Juliana Galera; Oliveira, Rafael de Novaes; Rodrigues, Adriana Cândido; Achkar, Samira Maria.
Canine distemper (CD) is one of the most important infectious diseases in domestic dogs. In Brazil, CD is still the principal cause of mortality of dogs in some urban populations. Although different viral gene sequences have been identified for detecting the canine distemper virus (CDV), the N gene appears to be a better target for the amplification of specific fragments from all CDV strains. Using RT-PCR targeted to the CDV N gene, during the year 2014 we analyzed 190 central nervous system (CNS) samples of dogs from the state of São Paulo, Brazil, with symptoms suggestive of encephalitis and sent them to the Pasteur Institute for the diagnosis of rabies. Positivity for CD was over 50%, indicating that the disease remains an important cause of canine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canine Distemper; RT-PCR; Encephalitis; Diagnosis cinomose canina; RT-PCR; Encefalite; Diagnóstico.
Ano: 2016
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/31032

Detección y caracterización molecular del Virus X de la Papa (PVX) en regiones productoras de papa de Colombia
Provedor de dados: 100
Autores: Gil Ramírez,J.F; Cotes Torres,J.M; Marín Montoya,M.
El cultivo de papa (Solanum tuberosum L.) se ve afectado por diferentes virus que reducen su rendimiento y la calidad del tubérculo-semilla. Determinar la distribución y la variabilidad genética de dichos patógenos, es fundamental para los programas de manejo integrado y mejoramiento genético. En este trabajo se evaluó la presencia y la variabilidad molecular de aislados de PVX en cultivos de papa de los departamentos de Antioquia, Cundinamarca, Boyacá y Nariño (Colombia). La evaluación de presencia se realizó mediante pruebas de ELISA, mientras que el análisis de variabilidad incluyó la secuenciación de una región parcial de la cápsida viral en 14 aislamientos y de porciones de los genes codificantes para las proteínas RdRp y Tgb1 para dos aislamientos....
Tipo: Journal article
Palavras-chave: Alphaflexiviridae; ELISA; RT-PCR; Solanum tuberosum; Variabilidad genética.
Ano: 2012
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522012000200001

Detección de potyvirus en plantas de pimiento (Capsicum annuum L.) y áfidos asociados al cultivo en Cuba
Provedor de dados: 100
Autores: Arana Labrada,Franklyn; Martínez Rivero,María A; Piñol Pérez,Bertha; Duarte Martínez,Leticia; Pacheco Sánchez,Ronal; Quiñones Pantoja,Madelaine.
El objetivo del trabajo fue detectar, mediante RT-PCR con cebadores genéricos, la presencia de potyvirus en plantas de pimiento (Capsicum annuum L.) con síntomas de moteado y en áfidos asociados al cultivo en los principales polos productivos de las regiones oriental, central y occidental de Cuba. Se extrajo el ARN total y se sintetizó el ADNc de las muestras colectadas. Se identificaron los áfidos como Myzus persicae (Sulzer). Se logró la detección de potyvirus en las muestras de las tres regiones, lo que demuestra que los potyvirus están ampliamente distribuidos en las áreas de producción del cultivo del pimiento en el país.
Tipo: Journal article
Palavras-chave: Myzus persicae; Pimiento; Potyvirus; RT-PCR.
Ano: 2015
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522015000300009

EVALUACIÓN DE LOS PARÁMETROS ANALÍTICOS PARA LA DETECCIÓN MOLECULAR DE POTYVIRUS QUE AFECTAN AL CULTIVO DEL PIMIENTO EN CUBA
Provedor de dados: 100
Autores: Díaz,Acela; Quiñones,Madelaine; Hernández,Annia; del Barrio,Gloria.
El género Capsicum (Solanaceae), es originario del continente americano y comprende alrededor de 25 especies, de las cuales cinco son cultivables. Las condiciones ambientales en que se desarrolla esta hortaliza, su forma de explotación, así como los tipos y variedades utilizadas, han traído consigo una amplia diversidad en cuanto a plagas y epifitias que causan importantes mermas en su producción. Entre ellas, las enfermedades virales constituyen la principal dificultad para el desarrollo del cultivo y de estas, las causadas por los miembros del género Potyvirus, se consideran entre las de mayor importancia económica. Este trabajo tiene como objetivo validar un ensayo de RT-PCR para el diagnóstico molecular de potyvirus, empleando cebadores genéricos. Los...
Tipo: Journal article
Palavras-chave: Potyvirus; Pimiento; Diagnóstico; RT-PCR.
Ano: 2010
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522010000200002

COMPARACIÓN DEL LÍMITE DE DETECCIÓN DE MÉTODOS DE ANÁLISIS DE ÁCIDOS NUCLEICOS PARA EL VIROIDE DEL ENANISMO DEL LÚPULO (HSVd) EN CÍTRICOS
Provedor de dados: 100
Autores: Soto,Marianela; González,Lien; Peralta,Esther Lilia; Duran-Vila,Nuria.
Se determinó y comparó el límite de detección de la electroforesis secuencial en geles de poliacrilamida (sPAGE), hibridación de ácidos nucleicos (NASH) y transcripción reversa-reacción en cadena de la polimerasa (RT-PCR) para el diagnóstico del viroide del enanismo del lúpulo (HSVd) en cítricos, basados en el análisis de ácidos nucleicos de plantas de cidro y pepino como amplificadores biológicos. El límite de detección de la sPAGE fue mayor (5x10-3) en plantas de pepino que en plantas de cidro (5x10-2); sin embargo, este fue 50 veces menos sensible que el de la NASH (10-4). El límite de detección de la RT-PCR en pepino fue el mayor de todos los métodos analizados (10-6). Dicho valor se potenció cuando los productos de RT-PCR fueron detectados por NASH en...
Tipo: Journal article
Palavras-chave: Viroide; HSVd; SPAGE; NASH; RT-PCR; Diagnóstico molecular.
Ano: 2007
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522007000200005

Characterization of Citrus tristeza virus isolates from Colombia
Provedor de dados: 100
Autores: Morales,Juan; Orlando,Acosta; Tamayo,Pablo; Peñaranda,José.
Isolates of Citrus tristeza virus around the world differ widely in their biological properties. These properties may depend on strain mixtures within individual trees. Monoclonal Antibody 13 (MCA13) is an antibody that detects almost all the severe strains causing Quick Decline and Stem Pitting CTV syndromes. The objectives of this work were: I) to determine the percentage of CTV isolates reactive to the MCA13 antibody in each Colombian region evaluated and II) to analyze the genetic diversity of isolates from these regions. In almost all the regions evaluated, CTV MCA13 reactive isolates were identified; however, only one was found in Mompox area. The coat protein gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and analyzed...
Tipo: Journal article
Palavras-chave: Virus cross-protection; Citrus tristeza disease; Citrus spp; RT-PCR; SSCP.
Ano: 2013
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522013000100006

DETECCIÓN RÁPIDA DEL VIRUS DE LA GASTROENTERITIS TRANSMISIBLE EN CERDO A TRAVÉS DE LA REACCIÓN EN CADENA DE LA POLIMERASA
Provedor de dados: 101
Autores: Rodríguez,Edisleidy; Betancourt,A; Barrera,Maritza; Lee,Changee; Yoo,Dongwan.
El objetivo de este estudio fue desarrollar una PCR anidada para la detección rápida del virus de TGE. Los cebadores fueron diseñados a partir de regiones altamente conservadas de varias secuencias de este virus incluidas en el análisis. Con el fin de lograr una buena especificidad de la técnica se incluyeron en el diseño, secuencias del virus de la diarrea epidémica porcina (PEDv), el cual pertenece a la misma familia viral y posee una estrecha relación genética con el virus de TGE. Como ambos virus producen los mismos signos clínicos fue importante obtener cebadores que no amplificaran el PEDv. Con los cebadores externos se obtuvo la amplificación de un fragmento de la talla esperada de 441 pb en todas las muestras evaluadas por RT-PCR, pero de muy baja...
Tipo: Journal article
Palavras-chave: Virus de la Gastroenteritis Transmisible del cerdo; RT-PCR; PCR anidado.
Ano: 2008
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2008000200011

Characterization of a new potyvirus causing mosaic and flower variegation in Catharanthus roseus in Brazil
Provedor de dados: 63
Autores: Maciel,Sheila Conceição; Silva,Ricardo Ferreira da; Reis,Marcelo Silva; Jadão,Adriana Salomão; Rosa,Daniel Dias; Giampan,José Segundo; Kitajima,Elliot Watanabe; Rezende,Jorge Alberto Marques; Camargo,Luis Eduardo.
Catharanthus roseus is a perennial, evergreen herb in the family Apocynaceae, which is used as ornamental and for popular medicine to treat a wide assortment of human diseases. This paper describes a new potyvirus found causing mosaic symptom, foliar malformation and flower variegation in C. roseus. Of 28 test-plants inoculated mechanically with this potyvirus, only C. roseus and Nicotiana benthamiana developed systemic mosaic, whereas Chenopodium amaranticolor and C. quinoa exhibited chlorotic local lesions. The virus was transmitted by Aphis gossypii and Myzus nicotianae. When the nucleotide sequence of the CP gene (768nt) was compared with other members of the Potyviridae family, the highest identities varied from 67 to 76 %. For the 3' UTR (286nt),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RT-PCR; Apocynaceae; Periwinkle; Diagnose; Genome sequencing.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000600013

Breakdown of resistance in sweet pepper against Pepper yellow mosaic virus in Brazil
Provedor de dados: 63
Autores: Gioria,Ricardo; Braga,Renato de Souza; Krause-Sakate,Renate; Roullier,Caroline; Rosa,Daniel Dias; Moura,Monika Fecury; Souza-Dias,José Alberto Caram de; Sawazaki,Haiko Enok; Camargo,Luis Eduardo Aranha; Rezende,Jorge Alberto Marques.
Plants of Capsicum annuum cv. Magali R, resistant to Pepper yellow mosaic virus (PepYMV), which showed severe yellow mosaic, leaf malformation and stunting were observed during the 2003/04 growing season in Lins, São Paulo State, Brazil. Potyvirus-like particles observed in leaf sap from infected plants under the electron microscope reacted with an antiserum against PepYMV in PTA-ELISA. In addition to C. annuum cv. Magali R, this potyvirus also infected systemically the resistant C. annuum cv. Rubia R. The nucleotide sequence of part of the CP gene of this potyvirus shared 96-98% identity with that of other PepYMV isolates. The partial nucleotide sequence of the 3' NTR showed 94-96% identity with that of PepYMV. These data indicate that this potyvirus is a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Potyvirus; RT-PCR; ELISA; Resistance gene.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000200017

Tomato chlorotic spot virus in hydroponically-grown lettuce in São Paulo State, Brazil
Provedor de dados: 102
Autores: Colariccio,Addolorata; Eiras,Marcelo; Chaves,Alexandre L. R.; Harakava,Ricardo; Chagas,César M.
In the regions of Campinas and Sumaré, São Paulo, Brazil, hidroponically grown crops of Lettuce (Lactuca sativa) cv. Verônica, which showed virus-like symptoms were examined by electron microscope, biological, serological and molecular tests. Pleomorphic, enveloped particles (80-100 nm in diameter) were always detected in these samples. Experimentally inoculated host plants, including lettuce, reacted with tospoviruses-induced symptoms. Some differences were observed in Gomphrena globosa, which reacted by showing local lesions and systemic mosaic. Two isolates of Tomato chlorotic spot virus (TCSV) were identified by DAS-ELISA and by RT-PCR. The sequencing and alignment of the RT-PCR coat protein amplified fragments have indicated a high degree of homology...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tospovirus; Serology; RT-PCR; Sequencing.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000300012

An isolate of Apple stem grooving virus associated with Cleopatra mandarin fruit intumescence
Provedor de dados: 102
Autores: Lovisolo,Osvaldo; Accotto,Gian Paolo; Masenga,Vera; Colariccio,Addolorata.
A citrus tatter leaf isolate (CTLV-Cl) of Apple stem grooving virus (ASGV) has been found to be associated with a fruit rind intumescence in Cleopatra mandarin (Citrus reshni) in Limeira (SP). The CTLV-Cl was mechanically transmitted to the main experimental herbaceous hosts of CTLV. Chenopodium quinoa and C. amaranticolor reacted with local lesions and systemic symptoms while other test plants reacted somewhat differently than what is reported for CTLV. A pair of primers designed for specific detection of ASGV and CTLV amplified the expected 801 bp fragment from the CTLV-Cl-infected plants. Typical capillovirus-like particles were observed by the electron microscope in experimentally infected C. quinoa and C. amaranticolor leaves.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus tatter leaf virus; Capillovirus; RT-PCR.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582003000100008

Sequence analysis of the capsid protein gene of an isolate of Apple stem grooving virus, and its survey in Southern Brazil
Provedor de dados: 102
Autores: NICKEL,OSMAR; FAJARDO,THOR V.M.; JELKMANN,WILHELM; KUHN,GILMAR B.
Apple stem grooving virus (ASGV) is one of the most important viruses infecting fruit trees. This study aimed at the molecular characterization of ASGV infecting apple (Malus domestica) plants in Santa Catarina (SC). RNA extracted from plants infected with isolate UV01 was used as a template for RT-PCR using specific primers. An amplified DNA fragment of 755 bp was sequenced. The coat protein gene of ASGV isolate UV01 contains 714 nucleotides, coding for a protein of 237 amino acids with a predicted Mr of approximately 27 kDa. The nucleotide and the deduced amino acid sequences of the coat protein gene showed identities of 90.9% and 97.9%, respectively, with a Japanese isolate of ASGV. Very high amino acid homologies (98.7%) were also found with Citrus...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Molecular characterization; Capillovirus; RT-PCR.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582001000300014

Detecção de Closterovirus em videira e caracterização parcial de um isolado do Grapevine leafroll-associated virus 3
Provedor de dados: 102
Autores: FAJARDO,THOR V.M.; KUHN,GILMAR B.; EIRAS,MARCELO; NICKEL,OSMAR.
O enrolamento da folha da videira (Vitis spp.) é uma doença causada por até oito vírus, Grapevine leafroll-associated virus (GLRaV) 1 a 8, sorologicamente distintos e associados ao floema de videiras infetadas. Neste trabalho, foram detectados GLRaV-1 e -3 por DAS-ELISA em 6,9 e 14,7% das amostras analisadas, respectivamente, e provenientes de duas importantes regiões vitícolas do Brasil (Serra Gaúcha e Vale do São Francisco). Os GLRaV-2, -5 e -7 não foram detectados. O GLRaV-3 também foi detectado por dot-ELISA e western blot, observando-se a provável proteína capsidial com cerca de 36 kDa. Um fragmento de 340 pb, compreendendo o terminal 3' do gene da polimerase viral de GLRaV-3, foi amplificado por PCR e seqüenciado. As seqüências de nucleotídeos e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitis; GLRaV-1; GLRaV-3; Sorologia; RT-PCR; RNA polimerase.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000100009

Diagnóstico biológico e molecular e análise da seqüência de nucleotídeos do gene da proteína capsidial de um isolado do Apple stem pitting virus
Provedor de dados: 102
Autores: Radaelli,Paula; Nickel,Osmar; Schons,Jurema; Aragão,Francisco J.L.; Fajardo,Thor V. M.
O Apple stem pitting virus (ASPV) foi detectado por RT-PCR em amostras de cultivares de pereiras européias (Pyrus communis L.) cvs. Starkrimson e Abate Fetel, e asiáticas (P. pyrifolia var. culta) cvs. Kousui e Housui coletadas no início do outono de 2003 em pomar da Estação Experimental da Embrapa Uva e Vinho, Vacaria, RS. Utilizando várias combinações de oligonucleotídeos, foram amplificados fragmentos de DNA de 269 e 1554 pb, este último contendo o gene completo (1131 nt) da proteína capsidial do ASPV. Outro fragmento amplificado de 291 pb compreende parte do gene da polimerase viral. Estes fragmentos constituem-se em um excelente instrumento de diagnóstico do ASPV em pereiras. A comparação das seqüências de nucleotídeos do gene da proteína capsidial do...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pyrus communis; P. pyrifolia; Malus spp.; Flexiviridae; ASPV; Dot-ELISA; Imunoblot; RT-PCR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582006000100009

Ocurrence of pepper yellow mosaic virus and cucumber mosaic virus on Capsicum chinense in the state of Amazonas, Brazil
Provedor de dados: 36
Autores: SOUZA,Leonor Cristina Silva; HANADA,Rogerio Eiji; ASSIS,Luiz Alberto Guimarães; CAMELO-GARCÍA,Viviana M.; REZENDE,Jorge Alberto Marques; YUKI,Valdir A.; KITAJIMA,Elliot W.
ABSTRACT The habanero chilli pepper, Capsicum chinense is an important crop in the Amazon Basin, mainly grown by small-scale producers. Capsicum chinense plants in an experimental field in the northern Brazilian state of Amazonas were found exhibiting characteristic symptoms of viral infection. Leaf sap from symptomatic plants examined under a transmission electron microscope revealed the presence of elongated flexuous particles and isometric particles. Using molecular assays, the viruses were identified as pepper yellow mosaic virus (PepYMV) and cucumber mosaic virus (CMV). Aphids, identified as Aphis gossypii, were found colonizing the C. chinense plants in the field and may be the vector for both PepYMV and CMV. We report the first occurrence of these...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Cucumovirus; Potyvirus; Transmission electron microscopy; RT-PCR; Aphis gossypii.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000100005

EXPRESIÓN DE DOS GENES CANDIDATOS A RESISTENCIA CONTRA LA BACTERIOSIS VASCULAR EN YUCA
Provedor de dados: 37
Autores: CONTRERAS NIETO,ELÍZABETH; LÓPEZ CARRASCAL,CAMILO E.
La yuca (Manihot esculenta Crantz, Euphorbiaceae) es el cuarto cultivo en importancia a nivel mundial como fuente de calorías para la población humana y cuya producción se ve afectada por la bacteriosis vascular, enfermedad ocasionada por Xanthomonas axonopodis pv. manihotis (Xam). La resistencia a enfermedades en plantas depende de la presencia de genes de resistencia (R), los cuales reconocen a los patógenos y simultáneamente permiten desencadenar la respuesta de defensa. A pesar de recientes esfuerzos encaminados a la identificación de genes R en yuca, aún no se ha logrado clonar el primer gen R en este cultivo. En el presente trabajo se estudió el perfil de expresión de dos Genes Candidatos a Resistencia (RGCs) asociados a QTLs de defensa contra la...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Xam; RGC; RT-PCR.
Ano: 2008
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2008000200012

SECUENCIACIÓN DEL GENOMA COMPLETO DEL Potato yellow vein virus (PYVV) EN TOMATE (Solanum lycopersicum) EN COLOMBIA
Provedor de dados: 37
Autores: MUÑOZ BAENA,Laura; GUTIÉRREZ SÁNCHEZ,Pablo Andrés; MARÍN MONTOYA,Mauricio.
RESUMEN Potato yellow vein virus (PYVV), es uno de los fitopatógenos más limitantes para la producción de papa en la región de Los Andes. A pesar que se le ha detectado infectando tomate en Colombia, el conocimiento de las características biológicas de las cepas presentes en este hospedante es muy limitado. En este estudio, utilizando secuenciación masiva de nueva generación (NGS), se obtuvo la secuencia completa de los tres segmentos genómicos del PYVV en plantas de tomate en Marinilla (Antioquia) y se evalúo la utilidad de tres juegos de cebadores para su detección mediante pruebas de RT-PCR convencional y en tiempo real (RT-qPCR). El genoma de la secuencia consenso presentó tamaños de 8043 nt (ARN1), 5346 nt (ARN2) y 3896 nt (ARN3) y se identificaron...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crinivirus; RT-PCR; RT-qPCR; Secuenciación de nucleótidos de alto rendimiento; Solanaceae.
Ano: 2017
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2017000100001

CARACTERIZACIÓN SEROLÓGICA Y MOLECULAR DE POTYVIRUS ASOCIADOS A LA VIROSIS DEL TOMATE DE ÁRBOL EN ANTIOQUIA (COLOMBIA)
Provedor de dados: 37
Autores: AYALA VÁSQUEZ,MARILUZ; GONZÁLEZ JAIMES,PAOLA; GUTIÉRREZ,PABLO ANDRÉS; COTES,JOSÉ MIGUEL; MARÍN MONTOYA,MAURICIO.
El tomate de árbol (Solanum betaceum) es una fruta andina altamente nutritiva, con propiedades organolépticas únicas para el procesamiento industrial y el mercado internacional. En Antioquia, Colombia, este cultivo presenta diferentes problemas fitosanitarios, siendo especialmente limitante la Virosis, causada por un complejo viral del que hacen parte entre otras, especies del género Potyvirus. En este trabajo se evaluaron mediante pruebas de ELISA los niveles de incidencia de este grupo viral en cultivos de siete zonas del departamento de Antioquia y se determinó la identidad taxonómica de dos de los potyvirus asociados al cultivo. Las pruebas serológicas muestran la presencia de potyvirus en seis de las siete zonas evaluadas, alcanzando niveles...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ELISA; Potyvirus; PVY; RT-PCR; Tamarillo.
Ano: 2010
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2010000300011

Detección de Cymbidium mosaic virus (CymMV) y Odontoglossum ringspot virus (ORSV) en orquídeas en México
Provedor de dados: 115
Autores: López-Hernández,M. Siboney; Palacios-Popo,P. Eloisa; De La Torre-Almaraz,Rodolfo.
En recorridos realizados en 2011 para la detección de enfermedades en orquídeas en invernaderos comerciales en el Estado de Morelos, se observaron plantas dañadas con moteados cloróticos, rayados tenues de color amarillo y manchas cloróticas y necróticas anulares. Hojas dañadas fueron recolectadas de plantas de los géneros Brassia, Brassocattleya, Cattleya, Encyclia, Epidendrum, Guariathe, Laelia, Oncidium, Shomburghia, Vainilla y Xilobium. Pruebas de detección serológica de proteína viral de doble anticuerpo (DAS)-ELISA) fueron realizadas con antisueros específicos para diversos virus que afectan a orquídeas. Al menos una planta de cada género presentó los virus Cymbidium mosaic virus (CymMV; Potexvirus) y Odontoglossum ringspot virus (ORSV; Tobamovirus),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Replicasa (RdRp); RT-PCR; Ornamentales.
Ano: 2014
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952014000500006

Detección cuantitativa del virus psorosis de cítricos mediante RT-PCR tiempo real
Provedor de dados: 115
Autores: Barragán-Valencia,Gabriela; Morales-Loredo,Alberto; Álvarez-Ojeda,M. Genoveva; Peña-del Río,M. Ángeles; Quintero-Zapata,Isela.
La RT-PCR tiempo real es útil para detectar el virus psorosis de cítricos (CPsV) y además permite evaluar la concentración viral que se puede usar para estudiar algunos aspectos de la enfermedad. En este trabajo se implementó un protocolo de RT-PCR tiempo real para la detección cuantitativa del CPsV, por lo que se diseñó un conjunto de iniciadores y una sonda Taqman con fundamento en una secuencia de 559 bases del gen responsable de la síntesis de la proteína de la cápside del CPsV procedente de tejido infectado de un árbol de campo. En el desarrollo de la curva estándar se obtuvo una R² = 0.998 de la cantidad de ARN molde con respecto a los valores de C T y eficiencia de amplificación de 94.5%. Se determinó la concentración viral en muestras de campo y se...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CPsV; Concentración viral; Descortezamiento; Manchas anulares; RT-PCR.
Ano: 2008
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952008000200010

Detección del Prunus necrotic ringspot virus en durazno (Prunus persica (L.) en México y caracterización molecular de su componente ARN-3
Provedor de dados: 115
Autores: De La Torre-Almaraz,Rodolfo; Sánchez-Navarro,Jesús; Pallás,Vicente.
Durante recorridos realizados de 2008 a 2012 en huertas comerciales de durazno en el Estado de México, estados de Morelos y Puebla, se observaron daños foliares de moteado amarillo, anillos cloróticos, patrones lineales y mosaico. Se transmitió mecánicamente un virus de macerados de hojas de durazno con daños de moteado amarillo, a plántulas de varias especies de tabaco causando manchas blanquecinas. En diagnósticos serológicos (DAS-ELISA) y de hibridación tipo dot-bot, utilizando ribosondas marcadas con digoxigenina, para la detección de seis virus que infectan al durazno, se detectó sólo al virus Prunus necrotic ringspot virus (PNRSV. Ilarvirus) en las muestras recolectadas con síntomas. El análisis electroforético de ARN-dc de origen viral, obtenido de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Moteado amarillo; Ilarvirus; RT-PCR; Diagnóstico.
Ano: 2014
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952014000600002

Detecção do vírus da cinomose canina por RT-PCR utilizando-se oligonucleotídeos para os genes da fosfoproteína, hemaglutinina e neuraminidase
Provedor de dados: 45
Autores: Pozza,M.; Simonetti,A.B.; Esteves,P.A.; Rijsewijk,F.A.M.; Roehe,P.M.
Empregou-se a técnica de reação em cadeia pela polimerase precedida de transcrição reversa para detecção do vírus da cinomose canina (CC). Para a padronização da técnica foram selecionados quatro pares de oligonucleotídeos (P1, P2, N1, H1), baseados em seqüências dos genes da fosfoproteína, neuraminidase e hemaglutinina, sendo utilizadas três cepas vacinais de vírus da CC como controles positivos. Foram analisadas três amostras isoladas de cães com cinomose e quatro amostras provenientes de cães com suspeita clínica de cinomose. Não houve amplificação nas amostras com suspeita clínica da doença. Os resultados obtidos com os oligonucleotídeos P1 e N1 foram superiores aos de H1. Os oligonucleotídeos P2 foram considerados inapropriados para a detecção do...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cão; Cinomose; RT-PCR; Polimorfismo.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000500010

Detecção de ácidos nucléicos de Brucella spp., Leptospira spp., herpesvirus bovino e vírus da diarréia viral bovina, em fetos bovinos abortados e em animais mortos no perinatal
Provedor de dados: 45
Autores: Cortez,A.; Castro,A.M.G.; Heinemann,M.B.; Soares,R.M.; Leite,R.C.; Scarcelli,E.; Genovez,M.E.; Alfieri,A.A.; Richtzenhain,L.J.
Samples of 114 bovine fetuses and 10 calves, which dead in perinatal period, were examined for detection of DNA. The most common detected agent was Brucella spp. in 17 samples (13.7%) followed by Leptospira spp. in 4 cases (3.2%),bovine herpesvirus (BHV) and bovine viral diarrhea (BVDV) in 3 animals (2.4%) each, and 1 for the association of BVDV and BHV. In 77.4 % (96/124) of the samples it was not possible to detect any agent.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Aborto; Feto; PCR; RT-PCR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600036

Avaliação da urina e de leucócitos como amostras biológicas para a detecção ante mortem do vírus da cinomose canina por RT-PCR em cães naturalmente infectados
Provedor de dados: 45
Autores: Negrão,F.J.; Alfieri,A.A.; Alfieri,A.F.
Urine and leucocytes were comparatively evaluated as clinical samples for ante mortem detection of the canine distemper virus (CDV) by a reverse transcription-polymerase chain reaction (RT-PCR) assay. One hundred and eighty eight dogs with clinical symptoms of distemper, were distributed in three groups. The group A was constituted of 93 dogs with systemic signs of distemper; the group B by 11 dogs with neurological signs, and the group C by 84 dogs that presented simultaneously systemic and neurological signs. In 66.5% (125/188) of the dogs was amplified an amplicon with 287 base pair of the CDV nucleoprotein gene. In 60.8% (76/125) of the animals the CDV was detected simultaneously in the urine and leucocytes, and in 39.2% (49/125) of the dogs just a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cão; Cinomose; Diagnóstico; RT-PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000100042

Immunohistochemical and molecular expression of laminin-332 gamma-2 chain in canine mammary tumors
Provedor de dados: 45
Autores: Zuccari,D.A.P.C; Castro,R; Jardim,B.V; Mancini,U.M; Polachini,G.M.
Forty-eight cases of canine mammary cancer were investigated to evaluate the immunohistochemical distribution of the γ2 chain of laminin-332. Tumor cells were compared to a pool of normal mammary tissues using quantitative RT-PCR. The western blot was performed in eight tumor samples as complementary test to evaluate protein integrity. Immunohistochemistry experiments showed negative, focal, and weak expression of laminin-332 γ2 in tumors with the worst prognosis. Quantitative PCR revealed downregulation of the gene in 27 (56.2%) of the animals. Out of the 16 dogs with γ2 chain overexpression, seven were still alive. The western blot results showed bands generation of 36, 50, and 98kDa, suggesting degradation of laminin-332 γ2 in malignant tumors. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Adhesion molecule; Cancer; Western blot; RT-PCR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000100005

Cloprostenol administration in the first week postpartum reduces expression of oxytocin receptors in the endometrium in Holstein-Zebu cows
Provedor de dados: 45
Autores: Camargos,A.; Wohlres-Viana,S.; Costa,I.F.; Camargo,L.S.; Ferreira,J.C.; Ramos,A.A.; Oba,E.
ABSTRACT The present study investigated the hormonal profile and expression of prostaglandin F2α (PGF2α), oxytocin and estrogen receptors in uterine tissues of postpartum cows treated with cloprostenol. Twenty Holstein-Zebu crossbred cows were treated with saline solution (treatment CONT) or cloprostenol (treatment CLO), both administered two and five days postpartum. Blood samples were collected on days two, seven, 14, 21 and 28 postpartum for progesterone, PGF2α metabolite (PGFM) and estradiol determination, and endometrial biopsy was performed in order to quantify the expression of oxytocin receptor (OXTR), prostaglandin F receptor (PTGFR) and estrogen receptor 1 (ERS1) genes. In the CLO treatment, expression of OXTR was reduced (P&lt;0.05) but no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PTGFR; ESR1; Progesterone; Estradiol; RT-PCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000400821

Lesões histológicas no sistema nervoso central de cães com encefalite e diagnóstico molecular da infecção pelo vírus da cinomose canina
Provedor de dados: 45
Autores: Gebara,C.M.S.; Wosiacki,S.R.; Negrão,F.J.; Alfieri,A.A.; Alfieri,A.F.
Dez amostras de urina de cães que apresentavam sinais clínicos sistêmicos e neurológicos indicativos da infecção pelo vírus da cinomose canina (CDV) foram analisadas pela técnica da reação em cadeia pela polimerase precedida de transcrição reversa (RT-PCR) para a detecção do RNA do CDV. O exame histopatológico foi realizado em fragmentos de cérebro, cerebelo e bexiga. Como controle negativo foram colhidos fragmentos de órgãos e urina de quatro cães sem sinais clínicos de doença infecciosa e que morreram por outras causas. Todos os cães (9/10) positivos em RT-PCR apresentaram alterações histológicas no cérebro e cerebelo, características de encefalite aguda (5/9) ou crônica (4/9) compatíveis com as causadas pelo CDV. Um dos cães com alterações clínicas...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Cão; Cinomose canina; Vírus da cinomose canina; RT-PCR; Histopatologia.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000200005

Apoptose e expressão de VP2 e GAPDH na infecção precoce pelo vírus da doença infecciosa da bursa de Fabricius em pintos SPF
Provedor de dados: 45
Autores: Batista,J.J.; Martins,A.S.; Moro,L.; Resende,J.S.; Martins,N.R.S.; Vasconcelos,A.C.
Vinte e nove pintos SPF de um dia foram inoculados com o vírus da doença infecciosa da bursa de Fabricius (VDIB) para avaliar a ocorrência precoce de apoptose e a expressão da proteína viral 2 (VP2) e da enzima gliceraldeído fosfato dehidrogenase (GAPDH). Os animais foram distribuídos em cinco grupos: 1-controle; e 2 a 5- com 24, 48, 72 e 96 horas pós-inoculação, respectivamente. Fragmentos da bursa de Fabricius foram colhidos para processamento histológico e extração de RNA. Lâminas coradas em HE e TUNEL (marcação in situ da fragmentação do genoma com transferase terminal de deoxinucleotídeo) foram utilizadas na morfometria do índice apoptótico. Amostras de mRNA foram testadas para a expressão dos genes VP2 e GAPDH utilizando-se transcrição reversa e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptose; IBDV; Bursa de Fabricius; RT-PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000200007

First molecular and serological detection of Epizootic Hemorrhagic Disease virus in white tailed deer ( Odocoileus virginianus ) from Tamaulipas, Mexico
Provedor de dados: 45
Autores: Merino,J.O.; Cruz,NI. De la; Galvan,G.; León,A.P. De; Burnes,J.
ABSTRACT Epizootic hemorrhagic disease viruses (EHDV) are dsRNA arboviruses transmitted by biting midges of the genus Culicoides that cause disease in domestic and wild ruminants. Epizootic hemorrhagic disease (EHD) is considered the most important infectious disease of white tailed deer (WTD) in North America, some studies in Northeast Mexico reported EHDV-seropositive WTD and EHDV-infected Culicoides vectors. The increasing population of WTD that share habitat with livestock in Northeast México highlights the importance of EHD for the livestock industry in the transboundary region with the U.S. One hundred and twenty two samples from WTD in Tamaulipas state, Mexico were tested by ELISA and RT-PCR for EHDV antibodies and nucleic acid, respectively. Twelve...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Deer; Detection; EHDV; RT-PCR; Serology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100077

RT-PCR em pools de soros sangüíneos para o diagnóstico da infecção aguda e de animais persistentemente infectados pelo vírus da diarréia viral bovina
Provedor de dados: 45
Autores: Pilz,D.; Alfieri,A.F.; Lunardi,M.; Alfieri,A.A.
Utilizou-se a técnica da RT-PCR para a detecção da região 5' UTR do genoma do vírus da diarréia viral bovina (BVDV) em pools de soros sangüíneos provenientes de um rebanho, constituído por 226 animais, que apresentava distúrbios da reprodução. A partir das amostras individuais de soro e de acordo com a categoria dos animais e o número de animais por categoria foram formados 10 pools (A a J) de soros. A primeira avaliação revelou a amplificação de um produto com 290pb nas reações referentes aos grupos D (35 vacas) e H (25 bezerros lactentes) que, após o desmembramento em amostras individuais, resultou na identificação de 11 vacas lactantes e 12 bezerros em amamentação positivos. Para a identificação de animais persistentemente infectados (PI) entre os 23...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Diarréia viral bovina; BVDV; RT-PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000100001

Expressão gênica de caspases 3 e 8 em timo e baço de ratas recém-desmamadas e imunossuprimidas por glicocorticóide
Provedor de dados: 45
Autores: Batista,J.J.; Martins,A.S.; Moro,L.; Vasconcelos,A.C.
Determinou-se a expressão gênica das caspases 3 e 8 mediante transcrição reversa de mRNA total e reação em cadeia da polimerase (RT-PCR) para avaliar a apoptose em timo e baço de ratas imunossuprimidas por glicocorticóides. Utilizou-se dexametasona para indução da apoptose e atrofia linfóide. Quarenta e cinco fêmeas Wistar recém-desmamadas foram separadas em três grupos: as ratas de A (n=18) e B (n=18) foram tratadas com 250 e 500mg de glicocorticóide, via intramuscular, respectivamente, e as do C (n=9) não foram tratadas. Após 24, 48 e 72 horas, seis animais de cada grupo tratado e três do controle foram anestesiados, pesados e sacrificados. O baço e o timo foram coletados e pesados. Fragmentos dos órgãos foram fixados em formol tamponado a 10% e...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rato; Apoptose; Dexametasona; Timo; Baço; Caspase 3; Caspase 8; RT-PCR.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000400006

Prevalence of hepatitis A virus in sea food in Iran
Provedor de dados: 45
Autores: Momtaz,H.; Rahimi,E.; Hajian,S.; Momeni,M.
The objective of this study was to determine the prevalence of Hepatitis A Virus (HAV) in sea food samples in the Isfahan and Shahrekord townships in Iran. From September 2010 to April 2011, a total of 300 samples of fresh fish, shrimp, crab and lobster were obtained from randomly selected retail stores in the Isfahan and Shahrekord townships in Iran. The samples were tested for the presence of HAV using a reverse transcriptase- polymerase chain reaction method. Out of the total number of samples examined, 8 (2.7%) were found to be positive for HAV. This virus was detected in 5% and 1.7% of fresh fish and shrimp, respectively. This study shows the importance of sea food as potential sources of HAV infection in people in Iran.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HAV; Sea food; RT-PCR; Iran.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400015

Perfil de restrição de um fragmento do gene da hemaglutinina amplificado pela RT-PCR a partir de estirpes vacinais e selvagens do vírus da cinomose canina
Provedor de dados: 45
Autores: Negrão,F.J.; Wosiacki,S.H.; Alfieri,A.A.; Alfieri,A.F.
Fragmentos com 721 pares de bases do gene da hemaglutinina (H) do vírus da cinomose canina (CDV), amplificados pela RT-PCR a partir de três estirpes vacinais (Snyder Hill, Onderstepoort e Rockborn) e de 27 amostras de campo, provenientes de cães com cinomose, foram clivados com as endonucleases Hinf I and Rsa I. A seleção das enzimas foi realizada por meio de análises in silico de seqüências do CDV depositadas em bases públicas de dados. Tanto as estirpes vacinais quanto as amostras selvagens do CDV apresentaram com a enzima Hinf I o mesmo perfil de restrição, confirmando a identidade do fragmento amplificado pela RT-PCR, uma vez que todas as estirpes com seqüências disponíveis (GenBank) têm sítios de restrição para essa enzima nas mesmas posições. O...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cão; Cinomose; Vírus da cinomose canina; Gene H; RT-PCR; RFLP.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600019

Detecção do gene da nucleoproteína do vírus da cinomose canina por RT-PCR em urina de cães com sinais clínicos de cinomose
Provedor de dados: 45
Autores: Gebara,C.M.S.; Wosiacki,S.R.; Negrão,F.J.; Oliveira,D.B de; Beloni,S.N.E.; Alfieri,A.A.; Alfieri,A.F.
A presença do vírus da cinomose canina (CDV) foi avaliada pela reação em cadeia da polimerase, precedida de transcrição reversa (RT-PCR), em 87 amostras de urina de cães que apresentavam sinais clínicos sugestivos de cinomose. Os animais foram distribuídos em três grupos. No grupo A foram incluídos 41 cães com alterações sistêmicas; no grupo B, 37 cães com alterações neurológicas; e no grupo C, nove cães com alterações sistêmicas e neurológicas simultâneas. O grupo D (controle) foi composto por 20 cães assintomáticos. Os resultados da RT-PCR foram correlacionados com a forma clínica da infecção e com as alterações hematológicas encontradas. Foi possível a amplificação parcial do gene da nucleoproteína do CDV em 41 (47,1%) das 87 amostras de urina...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cinomose canina; Vírus da cinomose; Urina; RT-PCR.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000400009

Detection of mouse hepatitis virus in mouse colonies using the nested polymerase chain reaction
Provedor de dados: 45
Autores: Cecílio,A.B.; Cândido,A.L.; Resende,M.; Bontempo,E.D.; Martins,A.S.
A reverse transcriptase polymerase chain reaction (RT-PCR) to detect mouse hepatitis virus (MHV) in hepatic tissue was developed. To circumvent possible failures in RT-PCR amplifications, a second round of PCR with internal primers was used to confirm the specificity and increase the sensitivity of the test. Using this method specific amplification of MHV sequences was observed in 18 out of 20 mouse colonies examined.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mouse hepatitis virus; RT-PCR.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400003

Molecular analysis of the N gene of canine distemper virus in dogs in Brazil
Provedor de dados: 45
Autores: Castilho,J.G.; Brandão,P.E.; Carnieli Jr,P.; Oliveira,R.N.; Macedo,C.I.; Peixoto,Z.M.P.; Carrieri,M.L.; Kotait,I.
Eleven central-nervous-system samples collected from stray dogs between 2000 and 2004 were found positive by RT-PCR, which amplified a 480bp fragment of the N gene of canine distemper virus (CDV). Phylogenetic analysis based on partial N-gene sequences showed four major clusters. All dog strains segregated into cluster I, with a mean nucleotide identity of 95.8% and 95.6% with the Onderstepoort and Lederle vaccine strains, respectively. Cluster II contained all the raccoon-related strains, cluster III Orient strains and Cluster IV the Onderstepoort and Lederle vaccine strains, with a mean nucleotide identity of 99.7% between them. This is the first report of phylogenetic analysis of CDV strains in Brazil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Canine distemper virus; RT-PCR; Phylogeny.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300016

Detection of Borrelia-specific 16S rRNA sequence in total RNA extracted from Ixodes ricinus ticks
Provedor de dados: 45
Autores: Radulović,Ž.; Milutinović,M.; Tomanović,S.; Mulenga,A.
A reverse transcriptase - polymerase chain reaction based assay for Borrelia species detection in ticks was developed. The method was based on amplification of 552 nucleotide bases long sequence of 16S rRNA, targeted by Borrelia specific primers. In the present study, total RNA extracted from Ixodes ricinus ticks was used as template. The results showed higher sensitivity for Borrelia detection as compared to standard dark-field microscopy. Method specificity was confirmed by cloning and sequencing of obtained 552 base pairs long amplicons. Phylogenetic analysis of obtained sequences showed that they belong to B. lusitaniae and B. afzelii genospecies. RT-PCR based method presented in this paper could be very useful as a screening test for detecting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Borrelia; RT-PCR; 16S rRNA; Ixodes ricinus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400015

Effect of the standardized Cimicifuga foetida extract on Hsp 27 expression in the MCF-7 cell line
Provedor de dados: 48
Autores: Soler,Maritza C; Molina,Jessica L; Díaz,Hugo A; Pinto,Vivian C; Barrios,Yasenka L; He,Kan; Roller,Marc; Weinstein-Oppenheimer,Caroline R.
Cimicifuga foetida, an Asian Cimicifuga species, has been employed as a cooling and detoxification agent in traditional Chinese medicine since ancient times. For this herb, two cycloartane triterpene glycosides isolated from the rhizomes have demonstrated cytotoxicity on rat tumor and human cancer cell lines. Since human Hsp27 is increased in various human cancers and exhibits cytoprotective activity that affects tumorigenesis and the susceptibility of tumours to cancer treatment, the purpose of this research was to study the expression of this protein in MCF-7 breast cancer cells. To accomplish this aim, MCF-7 cells were exposed to different concentrations of Cimicifuga foetida extract showing a reduction in cell number measured by the sulforhodamine...
Tipo: Journal article
Palavras-chave: Cimicifuga foetida; Cytotoxicity; Hsp-27; Immunofluorescence; MCF-7 cells; RT-PCR.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300005

A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses
Provedor de dados: 48
Autores: MEISEL,LEE; FONSECA,BEATRIZ; GONZÁLEZ,SUSANA; BAEZA-YATES,RICARDO; CAMBIAZO,VERONICA; CAMPOS,REINALDO; GONZALEZ,MAURICIO; ORELLANA,ARIEL; RETAMALES,JULIO; SILVA,HERMAN.
Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the...
Tipo: Journal article
Palavras-chave: CDNA library; Fruit; Fruit trees; Functional genomics; Peach; Polysaccharides; Prunus persica; RNA isolation; Rosaceae; RT-PCR.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010

Hypercholesterolemia and tissue-specific differential mRNA expression of type-1 5'-iodothyronine deiodinase under different selenium status in rats
Provedor de dados: 48
Autores: DHINGRA,SANJIV; BANSAL,MOHINDER P.
Type-1 5'-iodothyronine deiodinase (5'-DI) is responsible for conversion of T4 to T3. Selenium (Se) is an integral part of this enzyme. Keeping in view the strong association between atherosclerosis and hypothyroidism, the present study examined the behavior of 5'-DI in liver, aorta and thyroid during hypercholesterolemia following different Se status, i.e., Se deficiency (0.02ppm), adequate (0.2ppm) and excess dose (1ppm) in SD male rats. Animals were fed a control or high-cholesterol diet (2%) for 1 and 2 months. 5'-DI activity and mRNA expression was measured by RIA and RT-PCR respectively. In liver and aorta, 5'-DI expression significantly decreased with the Se-deficient and the high-cholesterol diet. The trend was opposite in thyroid, i.e., mRNA...
Tipo: Journal article
Palavras-chave: Selenium; Hypercholesterolemia; 5'-DI; Cholesterol; RT-PCR.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200013

mRNA expression profile of selected oxygen sensing genes in the lung during recovery from chronic hypoxia
Provedor de dados: 48
Autores: Panariti,Alice; Miserocchi,Giuseppe; Rivolta,Ilaria.
This study analyzed the time dependence decay of the mRNA of selected genes important for the hypoxia response. The genes chosen were the two isoforms of hypoxia-inducible factors, the three isoforms of the prolyl hydroxylase domain protein, the vascular endothelial growth factor and endothelial nitric oxide synthase. mRNA and proteins were extracted from lungs obtained from control, hypoxic and 15 minutes normoxic recovered rats and analyzed by Real-time RT-PCR or by the Western Blot technique. Results indicated that in normoxia isoform 2á was the more represented hypoxia-inducible factor mRNA, and among the prolyl hydroxylase domain transcripts, isoform 3 was the least abundant. Moreover, in chronic hypoxia only hypoxia-inducible factor 1&amp;#945; and...
Tipo: Journal article
Palavras-chave: Chronic hypoxia; Hypoxia-inducible Factor; Prolyl Hydroxylase Domain proteins; Lung; RT-PCR.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200008

Isolation, Identification and Molecular Characterization of Highly Pathogenic Newcastle Disease Virus From Field Outbreaks
Provedor de dados: 52
Autores: Ashraf,Asma; Shah,Muhammad Salah-ud-Din; Habib,Mudasser; Hussain,Mujahid; Mahboob,Shahid; Al-Ghanim,Khalid.
ABSTRACT Newcastle disease (ND) is a major infectious disease of the poultry caused by a virulent strain of Avian Paramyxovirus - 1, that is a single strand non-segmented negative sense RNA virus. ND virus is major threat to the poultry industry in many countries of the world. The study was aimed to isolate and identify Newcastle disease virus (NDV) by using a haemagglutination inhibition (HI) test and reverse transcription-polymerase chain reaction (RT-PCR) assay. A total 100 samples of infected and dead birds were collected from different poultry farms. The weight of the birds was ranged 1000-1200g. The birds were divided into 3 groups. Haemagglutination assay (HA) was performed to detect the presence of NDV in suspension of infected homogenized tissues...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Newcastle disease; Epidemic; Molecular characterization; Avian virus; RT-PCR.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100332

Molecular epidemiology of the human group A rotavirus in the Paraná State, Brazil
Provedor de dados: 52
Autores: Santos,Jucélia Stadinicki dos; Alfieri,Alice Fernandes; Leite,José Paulo Gagliardi; Skraba,Irene; Alfieri,Amauri Alcindo.
From January/2000 to December/2003, 550 diarrheic fecal samples from the children and adults were collected in several geographical regions of Paraná State, Brazil. The enzyme immunoassay showed 120 (21.8%) samples positive for the group A rotaviruses. One hundred and fourteen samples were genotyped by multiplex-nested-PCR assay. The highest frequency (77.5%) of the positive samples (n=93) was observed in the children under 5 years old. Rotavirus diarrhea was more frequent in the cold and dry seasons of the four evaluated years. The most frequent genotypes were: G1 (50.9%), G4 (9.6%), G9 (7.0%), G2 (1.7%), G3 (0.9%), P[ 8] (71.9%), and P[ 4] (3.5%). The P[ 8] G1 (46.5%) and P[ 8] G4 (9.6%) were the main combinations found to P and G genotypes. The mixed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrhea; Rotavirus; Diagnostic; PAGE; RT-PCR; Genotypes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200008

Expression of Ghrelin and GHSR-1a in Long Term Diabetic Rat's Kidney
Provedor de dados: 52
Autores: Yildirim,Aysegul Burcin; Karabulut,Derya; Dundar,Munis; Ulusoy,Hasan Basri; Sonmez,Mehmet Fatih.
The aim of this work was to study the relative ghrelin and growth hormone secretagogue receptor (GHS-R)1a gene expression in the kidney of long-term diabetic rats. Forty male Wistar albino rats were divided into four groups: C- control group, DI- one month diabetic rats group, DII- two months diabetic rats group, and DIII- three months diabetic rats group. Diabetes was induced by streptozotocin STZ (40mg/kg i.p). The rats were decapitated under ketamine anesthesia and their kidney tissues were removed. Tissue GHS-R mRNA levels, ghrelin expression, and histopathological damage scores were compared. Dilatation in the distal tubules, epithelial desquamation into the lumen of the tubules and transparent tubules showing glycogen vacuolation were observed in all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes mellitus; Ghrelin; GHSR-1a; Kidney; Immunohistochemistry; RT-PCR.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100300

RT-PCR for confirmation of echovirus 30 isolated in Belém, Brazil
Provedor de dados: 55
Autores: Gomes,Maria de Lourdes C.; Ferreira,Lauze Lee A.; Gomes,Rodrigo Henryque; Lamarão,Letícia M.; Silveira,Edna da; Rodrigues,Lílian da Silva; Silva,Cláudio Ferreira da; Almeida,Eduardo Ribeiro de.
Echovirus (Echo) 30 or human enterovirus B is the most frequent enterovirus associated with meningitis cases. Epidemics and outbreaks of this disease caused by Echo 30 have occurred in several countries. In Brazil, Echo 30 has been isolated from sporadic cases and outbreaks that occurred mainly in the south and southeast regions. We used RT-PCR to examine Echo 30 isolates from meningitis cases detected from March 2002 to December 2003 in Belém, state of Pará, in northern Brazil. The patients were attended in a Basic Health Unit (State Health Secretary of Pará), where cerebrospinal fluid (CSF) was collected and stored in liquid nitrogen. Weekly visits were made by technicians from Evandro Chagas Institute to the health unit and samples were stored at -70ºC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Echovirus 30; Aseptic meningitis; RT-PCR; Belém; Brazil.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000400006

Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples
Provedor de dados: 55
Autores: Macedo,Carla Isabel; Carnieli Jr,Pedro; Brandão,Paulo Eduardo; Rosa,Elizabeth S. Travassos da; Oliveira,Rafael de Novaes; Castilho,Juliana Galera; Medeiros,Rita; Machado,Rosangela Rocha; Oliveira,Rosely Cerqueira de; Carrieri,Maria Luiza; Kotait,Ivanete.
Rapid diagnosis of rabies in suspected human cases influences post-exposure prophylaxis for potential contacts of the patient and ensures appropriate patient management. Apart from the central nervous system (CNS), rabies virus (RABV) is usually present in small sensory nerves adjacent to hair follicles of infected humans. We used an RT-PCR, with primers targeted to the 3' terminal portion of the nucleoprotein gene (N), to test neck-skin samples of nine patients who had rabies in order to validate a diagnostic method that could serve as an additional tool for rabies diagnosis, particularly in antemortem samples. Six of eight postmortem samples were found to be positive for rabies by RT-PCR, and one of two samples collected antemortem was positive with this...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human rabies; Neck-skin; Diagnosis; RT-PCR; DNA sequencing.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500008

Virus isolation and genotype identification of human respiratory syncytial virus in Guizhou Province, China
Provedor de dados: 55
Autores: Zhao,Ting; Ye,Zhixu; Wang,Binlin; Cui,Yuxia; Nie,Yingjie; Yang,Bin; Chen,Kun; Zhang,Hua; Hu,Fangfang; Yu,Fuxun.
ABSTRACT To investigate the genetic variation and molecular epidemiology characteristics of Human Respiratory Syncytial Virus (HRSV) in Guizhou Province, nasopharyngeal aspirates were collected from patients with acute respiratory infection (ARI) in Guizhou Provincial People's Hospital, from December 2017 to March 2018, and inoculated to Hep-2 cells to isolate HRSV. Cells that showed cytopathic effect (CPE) were then confirmed by indirect immunofluorescence assay and reverse transcription. The sequence of the PCR products was determined for HRSV isolates, and the genetic variation was analyzed. Out of 196 nasopharyngeal aspirate samples, HRSV were isolated in 39. The second hypervariable region at the 3' terminal of glycoprotein gene (HVR2) sequence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human respiratory syncytial virus; Virus isolation; RT-PCR; HVR2; Phylogenetic analysis; Molecular epidemiology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000600427

Comparison of four extraction methods to detect hepatitis A virus RNA in serum and stool samples
Provedor de dados: 55
Autores: Paula,Vanessa Salete de; Villar,Livia Melo; Gaspar,Ana Maria Coimbra.
The efficiency of extraction methods for hepatitis A virus (HAV) RNA in clinical samples is of great importance for molecular diagnosis, especially in regions endemic for HAV, such as Brazil. We compared the efficiency of four different extraction techniques in serum and stool samples for the detection of hepatitis A virus by reverse transcription PCR (RT-PCR). We used PCR to analyse serum and stool samples of 12 patients who were referred to the Brazilian Reference Center for Viral Hepatitis (BRCVH) in Rio de Janeiro. The methods tested were Proteinase K, Silica, TRIzol and Guanidine isothiocyanate. Proteinase K extraction was the best method for serum samples; it detected the HAV-RNA in 11 of the 12 samples. The guanidine isothiocyanate method was the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HAV; Extraction methods; Serum and stool; RT-PCR.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000200007

Dynamic expression of desmin, α-SMA and TGF-β1 during hepatic fibrogenesis induced by selective bile duct ligation in young rats
Provedor de dados: 56
Autores: Gonçalves,J.O.; Tannuri,A.C.A.; Coelho,M.C.M.; Bendit,I.; Tannuri,U.
We previously described a selective bile duct ligation model to elucidate the process of hepatic fibrogenesis in children with biliary atresia or intrahepatic biliary stenosis. Using this model, we identified changes in the expression of alpha smooth muscle actin (α-SMA) both in the obstructed parenchyma and in the hepatic parenchyma adjacent to the obstruction. However, the expression profiles of desmin and TGF-β1, molecules known to be involved in hepatic fibrogenesis, were unchanged when analyzed by semiquantitative polymerase chain reaction (RT-PCR). Thus, the molecular mechanisms involved in the modulation of liver fibrosis in this experimental model are not fully understood. This study aimed to evaluate the molecular changes in an experimental model...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha smooth muscle actin; Desmin; Hepatic fibrogenesis; Selective bile duct ligation; RT-PCR; TGF-β1.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000850

In vitro cytotoxicity of the LDE: daunorubicin complex in acute myelogenous leukemia blast cells
Provedor de dados: 56
Autores: Dorlhiac-Llacer,P.E.; Marquezini,M.V.; Toffoletto,O.; Carneiro,R.C.G.; Maranhão,R.C.; Chamone,D.A.F.
Acute myelogenous leukemia (AML) blast cells show high-affinity degradation of low-density lipoprotein (LDL), suggesting an increased expression of cellular LDL receptors. LDE is a lipid microemulsion easily synthesized in vitro which is known to mimic the metabolic pathway of LDL. We used LDE as a carrier for daunorubicin and assayed the cytotoxicity of the complex using AML blast cells since RT-PCR analysis showed that AML cells express LDL receptor mRNA. The LDE:daunorubicin complex killed 46.7% of blast cells and 20.2% of normal bone marrow cells (P&lt;0.001; Student t-test). Moreover, this complex destroyed AML blast cells as efficiently as free daunorubicin. Thus, LDE might be a suitable carrier of chemotherapeutic agents targeting these drugs to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Acute myelogenous leukemia; Daunorubicin; Low-density lipoprotein; LDE complex; RT-PCR.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000004

Sm14 gene expression in different stages of the Schistosoma mansoni life cycle and immunolocalization of the Sm14 protein within the adult worm
Provedor de dados: 56
Autores: Brito,C.F.A.; Oliveira,G.C.; Oliveira,S.C.; Street,M.; Riengrojpitak,S.; Wilson,R.A.; Simpson,A.J.G.; Correa-Oliveira,R.
Sm14 is a 14-kDa vaccine candidate antigen from Schistosoma mansoni that seems to be involved in cytoplasmic trafficking of fatty acids. Although schistosomes have a high requirement for lipids, they are not able to synthesize fatty acids and sterols de novo. Thus, they must acquire host lipids. In order to determine whether Sm14 is present in different stages of the life cycle of the parasite, we performed RT-PCR. Sm14 mRNA was identified in all stages of the life cycle studied, mainly schistosomulum, adult worm and egg. Additionally, we used a rabbit anti-Sm14 polyclonal antibody in an indirect immunofluorescence assay to localize Sm14 in adult worm sections. The basal lamella of the tegument and the gut epithelium were strongly labeled. These tissues...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sm14; Fatty acid-binding protein; FABP; Immunolocalization; RT-PCR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300014

CaV 3.1 and CaV 3.3 account for T-type Ca2+ current in GH3 cells
Provedor de dados: 56
Autores: Mudado,M.A.; Rodrigues,A.L.; Prado,V.F.; Beirão,P.S.L.; Cruz,J.S.
T-type Ca2+ channels are important for cell signaling by a variety of cells. We report here the electrophysiological and molecular characteristics of the whole-cell Ca2+ current in GH3 clonal pituitary cells. The current inactivation at 0 mV was described by a single exponential function with a time constant of 18.32 ± 1.87 ms (N = 16). The I-V relationship measured with Ca2+ as a charge carrier was shifted to the left when we applied a conditioning pre-pulse of up to -120 mV, indicating that a low voltage-activated current may be present in GH3 cells. Transient currents were first activated at -50 mV and peaked around -20 mV. The half-maximal voltage activation and the slope factors for the two conditions are -35.02 ± 2.4 and 6.7 ± 0.3 mV (pre-pulse of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium channel; T-type; Electrophysiology; RT-PCR; GH3 cells.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600020

Human group C rotavirus in children with diarrhea in the Federal District, Brazil
Provedor de dados: 56
Autores: Teixeira,J.M.S.; Camara,G.N.N.L.; Pimentel,P.F.V.; Ferreira,M.N.R.; Ferreira,M.S.R.; Alfieri,A.A.; Gentsch,J.R.; Leite,J.P.G.
Group C rotaviruses are fastidious in their in vitro cell culture requirements. Recent serosurveys indicate that antibody to group C rotavirus is present in 3-45% of the human population in certain geographic locations, suggesting that rotavirus group C infection is more prevalent than previously believed and that the low rate of detection of these agents is probably due to the lack of sensitive diagnostic assays. From March to December 1994, 406 fecal specimens were collected from children under five years of age who were outpatients at the emergency services of nine public hospitals in Brasília, Federal District, Brazil. In addition to the samples from children, one public outpatient unit requested virological investigation of a stool sample from an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Group C rotavirus; Diarrhea; HIV; RT-PCR; Probe hybridization.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100005

Up-regulated expression of CD147 gene in malignant bone tumor and the possible induction mechanism during osteoclast formation
Provedor de dados: 56
Autores: Qiao,Suchi; Liu,Chang; Xu,Weijie; AZhaTi,WuBuLi; Li,Cheng; Wang,Zhiwei.
It is increasingly evident that the microenvironment of bone can influence cancer phenotype in many ways that favor growth in bone. CD147, a transmembrane protein of the immunoglobulin (Ig) superfamily, was identified independently in different species and has many designations across different species. However, expression levels of CD147 mRNA in bone cancer have not been described. In this study, we have used real-time fluorescence quantification (RT-PCR) to demonstrate CD147 expression in malignant bone cancer and benign bone tumor tissues. The results suggested that the expression of CD147 gene was significantly up-regulated in malignant bone cancer. Moreover, we found that over-expressed RANKL progressively enhanced osteoclast formation up to 48 h,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone cancer; RT-PCR; Western blot; RANKL; Human.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900608

Kinetics of TNF-alpha and IFN-gamma mRNA expression in islets and spleen of NOD mice
Provedor de dados: 56
Autores: Ventura-Oliveira,D.; Vilella,C.A.; Zanin,M.E.; Castro,G.M.; Moreira Filho,D.C.; Zollner,R.L.
Insulin-dependent diabetes mellitus is caused by autoimmune destruction of pancreatic ß cells. Non-obese diabetic (NOD) mice spontaneously develop diabetes similar to the human disease. Cytokines produced by islet-infiltrating mononuclear cells may be directly cytotoxic and can be involved in islet destruction coordinated by CD4+ and CD8+ cells. We utilized a semiquantitative RT-PCR assay to analyze in vitro the mRNA expression of TNF-alpha and IFN-gamma cytokine genes in isolated islets (N = 100) and spleen cells (5 x 10(5) cells) from female NOD mice during the development of diabetes and from female CBA-j mice as a related control strain that does not develop diabetes. Cytokine mRNAs were measured at 2, 4, 8, 14 and 28 weeks of age from the onset of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; NOD mice; Cytokines; RT-PCR; Pancreatic islets; Time course.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100013

PPM1B and P-IKKβ expression levels correlated inversely with rat gastrocnemius atrophy after denervation
Provedor de dados: 56
Autores: Wei,Jian; Liang,Bing-Sheng.
Activated inhibitor of nuclear factor-κB kinase β (IKKβ) is necessary and sufficient for denervated skeletal muscle atrophy. Although several studies have shown that Mg2+/Mn2+-dependent protein phosphatase 1B (PPM1B) inactivated IKKβ, few studies have investigated the role of PPM1B in denervated skeletal muscle. In this study, we aim to explore the expression and significance of PPM1B and phosphorylated IKKβ (P-IKKβ) during atrophy of the denervated gastrocnemius. Thirty young adult female Wistar rats were subjected to right sciatic nerve transection and were sacrificed at 0 (control), 2, 7, 14, and 28 days after denervation surgery. The gastrocnemius was removed from both the denervated and the contralateral limb. The muscle wet weight ratio was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Muscular atrophy; Denervation; PPM1B; P-IKKβ; IKKβ; RT-PCR; Western blot.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800004

Acute promyelocytic leukemia: the study of t(15;17) translocation by fluorescent in situ hybridization, reverse transcriptase-polymerase chain reaction and cytogenetic techniques
Provedor de dados: 56
Autores: Chauffaille,M.L.L.F.; Figueiredo,M.S.; Beltrani,R.; Antunes,S.V.; Yamamoto,M.; Kerbauy,J.
Acute promyelocytic leukemia (AML M3) is a well-defined subtype of leukemia with specific and peculiar characteristics. Immediate identification of t(15;17) or the PML/RARA gene rearrangement is fundamental for treatment. The objective of the present study was to compare fluorescent in situ hybridization (FISH), reverse transcriptase-polymerase chain reaction (RT-PCR) and karyotyping in 18 samples (12 at diagnosis and 6 after treatment) from 13 AML M3 patients. Bone marrow samples were submitted to karyotype G-banding, FISH and RT-PCR. At diagnosis, cytogenetics was successful in 10 of 12 samples, 8 with t(15;17) and 2 without. FISH was positive in 11/12 cases (one had no cells for analysis) and positivity varied from 25 to 93% (mean: 56%). RT-PCR was done...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute promyelocytic leukemia; Karyotype; FISH; RT-PCR; PML/RARA genem rearrangement.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600006

Experimental infection with Brazilian Newcastle disease virus strain in pigeons and chickens
Provedor de dados: 58
Autores: Carrasco,Adriano de Oliveira Torres; Seki,Meire Christina; Benevenute,Jyan Lucas; Ikeda,Priscila; Pinto,Aramis Augusto.
Abstract This study was designed with the goal of adding as much information as possible about the role of pigeons (Columba livia) and chickens (Gallus gallus) in Newcastle disease virus epidemiology. These species were submitted to direct experimental infection with Newcastle disease virus to evaluate interspecies transmission and virus-host relationships. The results obtained in four experimental models were analyzed by hemagglutination inhibition and reverse transcriptase polymerase chain reaction for detection of virus shedding. These techniques revealed that both avian species, when previously immunized with a low pathogenic Newcastle disease virus strain (LaSota), developed high antibody titers that significantly reduced virus shedding after...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gallus gallus; Columba livia; Experimental infection; RT-PCR; Serology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100231

Detection of poliovirus type 2 in oysters by using cell culture and RT-PCR
Provedor de dados: 58
Autores: Vinatea,Cecília E.B.; Sincero,Thaís C.M.; Simões,Claúdia M.O.; Barardi,Célia R.M.
Shellfish are readily contaminated with viruses present in water containing sewage due to the concentrating effect of filter feeding. Enteroviruses are generally used as a model for the detection of viruses from shellfish due to their public health significance. In the present work, oysters were placed in glass aquaria containing seawater plus unicellular algae. Two experiments were performed: 1) oysters bioaccumulating four different poliovirus type 2 concentrations: 5 x 10(4), 2.5 x 10(4), 5 x 10³ and 5 x 10² PFU/mL during 20h; 2) oyster tissues directly inoculated with 6.0 x 10(5) and 1.0 x 10(5) PFU/mL. After viruses seeding, tissue samples were processed by an adsorption-elution-precipitation method. Positive controls were performed by seeding 6.0 x...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oysters; Poliovirus; Bioaccumulation; Cell culture; RT-PCR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100012

Virus isolation and molecular characterization of canine distemper virus by RT-PCR from a mature dog with multifocal encephalomyelit
Provedor de dados: 58
Autores: Amude,Alexandre Mendes; Carvalho,Glei dos Anjos; Alfieri,Amauri Alcindo; Alfieri,Alice Fernandes.
A case of multifocal distemper encephalomyelitis in a mature dog is described. In the presented case the ante mortem clinical diagnosis of canine distemper virus (CDV) infection could not be ideally performed due to the absence of typical signs of distemper, such as myoclonus and systemic signs accompanying the nervous signs. The definitive diagnosis of distemper encephalomyelitis was only carried out at post mortem through virus isolation in cell culture from fresh central nervous system (CNS) fragments and CDV nucleoprotein gene detection in the CNS by RT-PCR.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Canine distemper virus; Encephalomyelitis; RT-PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200031

Identification and molecular characterization of the infectious bursal disease virus (IBDV) from an outbreak in a broiler flock in midwestern Brazil
Provedor de dados: 58
Autores: Paula,Márcia Beatriz Cardoso de; Yokosawa,Jonny; Coutinho,Márcio Danilo Botrel; Silva,Paulo Lourenço; Ferraz,Rogério Avelar; Oliveira,Thelma Fátima Mattos; Queiróz,Divina Aparecida Oliveira.
In order to identify and characterize the agent of a suggestive clinical case of Gumboro disease (GD) that affected a 34-day-old broiler flock in Buriti Alegre (Goias State, Midwestern Brazil) in the year 2001, we carried out a combination of classic and modern virological methods. Histopathological analysis of the bursa revealed necrosis, presence of depleted follicles, some infiltration of heterophils, edema and formation of cystic cavities that are compatible with lesions observed in GD. Inoculation of embryonated eggs of specific pathogen-free (SPF) chickens with macerated bursa suspension resulted in embryo mortality and lesions which were also compatible with those caused by IBDV. A sample of bursa was submitted to a nested reverse...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken broiler flock; Gumboro disease; Infectious bursal disease virus; IBDV; RT-PCR; Molecular characterization.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300016

Human respiratory syncytial virus detection in children admitted at a community hospital in Botucatu, SP, Brazil
Provedor de dados: 58
Autores: Bosso,Patrícia Alves Ramos; Candeias,João Manuel Grisi; Paduan,Karina dos Santos; Ricchetti,Sandra Mara Queiroz; Miranda,Antero Frederico Macedo de; Rugolo,Lígia Maria Suppo de Souza; Durigon,Edison Luiz; Ventura,Armando Morais.
Human Respiratory Syncytial Virus (HRSV) is recognized as the single most important pathogen responsible for acute viral infection of the lower respiratory tract in young children. In this study, 84 samples from children under two years of age presenting acute respiratory symptoms were obtained from September 2000 to November 2001. Analysis by immunofluorescence and reverse-transcription followed by PCR, revealed that 18% (15/84) of all the samples were positive for HRSV, in which 80% (12/15) of the cases was observed in children under six months of age and the subgroups A and B co-circulated in the studied period. These are the first data obtained for Botucatu city and seasonality is evident by the higher circulation of the virus between May and July.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human Respiratory Syncytial Virus (HRSV); RT-PCR; Immunofluorescence; Seasonality.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300015

Sequencing and phylogenetic analysis of the infectious bursal disease virus isolates from outbreak in layer flocks in the state of Minas Gerais
Provedor de dados: 58
Autores: Dias,Camila Cristina Almeida; Souza,Fernanda de Oliveira; Silva,Elisa Monteiro Sant'Anna da; Eller,Monique Renon; Barrios,Priscilla Rochele; Santos,Bernadete Miranda dos; Moraes,Mauro Pires; Almeida,Márcia Rogéria de.
Sequencing and phylogenetic analysis based on the nucleotide sequence of the gene encoding VP2 protein was carried out in order to characterize the agent of two outbreaks of infectious bursal disease in layer flocks in the state of Minas Gerais in 2004. The results indicate the outbreaks could be related to the vaccinal virus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IBDV; RT-PCR; Molecular characterization; Phylogenic analysis; Infectious bursal disease.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100036

Evaluation of the effect of natural peptide 'Urocortin' on corticotrophin releasing factor (CRF) receptor expression in ND7/23 cells
Provedor de dados: 60
Autores: Mohiuddin,Omair Anwar; Biggs,Chris.
CRF receptors are involved in the stress management of the cells and are believed to have a cytoprotective role in the body. CRF receptors have been reported to be potential drug targets for the treatment of neurodegenerative disorders. The cell line used in the study is ND7/23 (mouse neuroblastoma and rat dorsal root ganglion neuron hybridoma). The aim of the study was to confirm the expression of CRF receptors in ND7/23 cells and to determine if urocortin (Ucn) can enhance the expression of CRF receptors. ND7/23 cells were cultured in RPMI 1640 media and cells grown after the second passage were used for the experiments. RNA was extracted from the cells and amplified by RT-PCR to confirm the presence of CRF receptors. The cells were then subjected to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urocortin; ND7/23 cells; Corticotrophin releasing factor; RT-PCR; Neuronal cells.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100233

Expression of EGF receptors in canine prostate with proliferative inflammatory atrophy and carcinoma
Provedor de dados: 65
Autores: Faleiro,Mariana Batista Rodrigues; Cintra,Lorena Cardoso; Jesuino,Rosália Santos Amorim; Araújo,Eugênio Gonçalves de; Rocha,Rafael Malagoli; Moura,Veridiana Maria Brianezi Dignani de.
ABSTRACT: Gene expression of ErbB1 and ErbB2, and immunostaining of EGFR (Her1) and Her2 (c-erbB-2) were evaluated in this study to ascertain whether these receptors are involved in the evolution of canine premalignant and malignant prostatic lesions, as proliferative inflammatory atrophy (PIA) and prostatic carcinoma (PC). With regards to the intensity of EGFR immunostaining, there was no difference between normal prostatic tissue and tissues with PIA or PC. In relation to Her2 immunostaining, there were differences between normal prostatic tissue and those with PIA and PC, as also differences between prostates with PIA and PC. There was no correlation between EGFR and Her2 immunostaining. ErbB1 gene product was detected in two normal tissue samples, in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth factor receptor; Her1; C-erbB-2; PIA; RT-PCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200553