Consulta: subjectFacets:"RT-qPCR"
Registros recuperados: 24
Data/hora: 06/06/2024 08:04:47

Total RNA quality of lyophilized and cryopreserved dormant grapevine buds
Provedor de dados: 69
Autores: García-Baldenegro,Claudia Vanessa; Vargas-Arispuro,Irasema; Islas-Osuna,María; Rivera-Domínguez,Marisela; Aispuro-Hernández,Emmanuel; Martínez-Téllez,Miguel Ángel.
Background Plant tissues must be preserved in their collection state, especially for genome-wide expression profile studies. Lyophilization is a feasible, affordable tool when fresh tissues cannot be shipped at ultralow temperatures from their origin to the place of analysis. In this study, the total RNA quality of dormant grapevine buds (Vitis vinifera L. cv. ‘Flame Seedless’) of freeze-dried samples stored at room temperature conditions was evaluated and compared to that of cryopreserved (-80°C) grapevine buds. Results Good yield and quality of RNA were obtained from freeze-dried dormant buds stored at room temperature for 0, 3 and 6 weeks after they were lyophilized. Further experiments confirmed that the extracted total RNA could be used for actin and...
Tipo: Journal article
Palavras-chave: Freeze-dried; RNA integrity number; RT-qPCR; Vitis vinifera L; Woody tissue.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200012

Gene expression analysis reveals important pathways for drought response in leaves and roots of a wheat cultivar adapted to rainfed cropping in the Cerrado biome
Provedor de dados: 74
Autores: Poersch-Bortolon,Liane Balvedi; Pereira,Jorge Fernando; Nhani Junior,Antonio; Gonzáles,Hebert Hernán Soto; Torres,Gisele Abigail Montan; Consoli,Luciano; Arenhart,Rafael Augusto; Bodanese-Zanettini,Maria Helena; Margis-Pinheiro,Márcia.
Abstract Drought limits wheat production in the Brazilian Cerrado biome. In order to search for candidate genes associated to the response to water deficit, we analyzed the gene expression profiles, under severe drought stress, in roots and leaves of the cultivar MGS1 Aliança, a well-adapted cultivar to the Cerrado. A set of 4,422 candidate genes was found in roots and leaves. The number of down-regulated transcripts in roots was higher than the up-regulated transcripts, while the opposite occurred in leaves. The number of common transcripts between the two tissues was 1,249, while 2,124 were specific to roots and 1,049 specific to leaves. Quantitative RT-PCR analysis revealed a 0.78 correlation with the expression data. The candidate genes were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 454 sequencing; Candidate genes; RT-qPCR; Triticum aestivum; Water deficit.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400629

Molecular cloning, characterization and expression analysis of a novel PDRG1 gene from black tiger shrimp (Penaeus monodon)
Provedor de dados: 74
Autores: Zhao,Chao; Dai,Wenting; Qiu,Lihua.
Abstract P53 And DNA Damage-Regulated Gene 1 (PDRG1) is a novel gene which plays an important role in chaperone-mediated protein folding. In the present study, the full-length complementary DNA (cDNA) sequence of the PDRG1 gene from Penaeus monodon (PmPDRG1) was cloned by the rapid amplification of cDNA ends (RACE) method. The cDNA of PmPDRG1 spans 1,613 bp, interrupted by only one short intron, and encodes a protein of 136 amino acids with calculated molecular weight of 15.49 kDa. The temporal expression profile of PmPDRG1 in different tissues and in different developmental stages of the ovary was investigated by real-time quantitative PCR (RT-qPCR). An RNA interference (RNAi) experiment was performed to study the relationship between P. monodon p53...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PDRG1; Gene cloning; RT-qPCR; Ovarian development; Black tiger shrimp.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100093

Considerações estatísticas na análise de dados de expressão gênica gerados pela técnica de RT-qPCR.
Provedor de dados: 14
Autores: LOBO, A. M. B. O.; LOBO, R. N. B.
A transcriptase reversa (RT) seguida de quantificação utilizando a técnica da reação em cadeia da polimerase (qPCR) em tempo real ou, simplesmente reverse transcription-qPCR (RT-qPCR), é um método in vitro para amplificação enzimática de sequências definidas de DNA complementar obtido a partir do RNA. A técnica de RT-qPCR é uma das mais utilizadas em experimentos de quantificação da expressão gênica por ser considerado um dos métodos mais sensível e específico de quantificação gênica dos que outros utilizados para esta finalidade. No entanto, para obter resultados, pela técnica de RT-qPCR, que possam ser adequadamente analisados é necessário que o experimento tenha um desenho experimental com tratamentos, ensaios, unidade experimental e delineamento bem...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: RT-qPCR; Expressão gênica; Expressão gênica relativa; RT-PCR; Genetic processes.; Genética animal; Genética molecular; Gene expression; Genetics.
Ano: 2014
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1007398

Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts
Provedor de dados: 5
Autores: Akcha, Farida; Barranger, Audrey; Bachere, Evelyne; Berthelin, C. Heude; Piquemal, D.; Alonso, P.; Sallan, R. Rondon; Dimastrogiovanni, G.; Porte, C.; Menard, Dominique; Szczybelski, A.; Benabdelmouna, Abdellah; Auffret, M.; Rouxel, Julien; Burgeot, Thierry.
Genitors of the Pacific oyster Crassostrea gigas were submitted during gametogenesis to a short pulse exposure to the herbicide diuron at a realistic environmental concentration. Histological analysis showed no effect of diuron on gametogenesis course, sex ratio and reproductive effort. A non-significant increase in testosterone and progesterone levels was observed in genitors exposed to the herbicide. At cell level, diuron exposure was shown to modulate the phagocytic activity of circulating hemocytes. The results of a transcriptional analysis showed that diuron affected the expression of genes belonging to functions known to play a major role during oyster gametogenesis such as gene transcription regulation, DNA replication and repair, DNA methylation...
Tipo: Text
Palavras-chave: Oyster; Pesticide; Reproduction; Histology; Gene expression; RT-qPCR; Steroid; Immunity.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00309/41983/83232.pdf

Quantification des bactéries histaminogènes et maîtrise de la formation d'histamine dans les produits marins par biopréservation
Provedor de dados: 5
Autores: Podeur, Gaetan.
Histamine-producing bacteria were isolated from naturally contaminated seafood, some of responsible for histamine-poisoning. At the same time, lactic acid bacteria were isolated on the same products and clustered according to their inhibitory activity against 4 histamine-producing species. Most of the strains were identified as Lactobacillus fuchuensis or Lactobacillus sakei. Twelve lactic acid bacteria were selected for challenge-test on canned, cooked or smoked tuna. Challenge-test combined with sensory analysis performed on cooked vacuum-packed tuna against Morganella psychrotolerans and Morganella morganii, demonstrated an important inhibitory effect on growth and production of histamine with L. sakei CNCM I-4707. Bacterial count was reduced by 3 to 5...
Tipo: Text
Palavras-chave: Biopréservation; Thon; Bactérie lactique; Intoxication histaminique; Morganella morganii; Morganella psychrotolerans; Lactobacillus sakei; Analyse sensorielle; PCR en temps réel; Étape d'enrichissement; RT-qPCR; Biopreservation; Tuna; Lactic acid bacteria; Scombroid poisoning; Morganella morganii; Morganella psychrotolerans; Lactobacillus sakei; Sensory analysis; Real-time PCR; Enrichment step; RT-qPCR.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00241/35203/33709.pdf

Metallothionein mRNA induction is correlated with the decrease of DNA strand breaks in cadmium exposed zebra mussels
Provedor de dados: 5
Autores: Vincent-hubert, Francoise; Chatel, Amelie; Gourlay-france, Catherine.
We have previously shown that cadmium (Cd) and Benzo[a]pyrene (BaP) induced early DNA damages in zebra mussels, and that the level of DNA strand breaks (SB) returned to a basal level after 3 days of exposure to Cd. The aim of the present study was to go further in the mechanisms of Cd and BaP detoxification. For that purpose, expression of genes encoding for metallothionein (MT), Aryl Hydrocarbon Receptor (AHR), P-gP, catalase, glutathione S-transferase and Heat shock protein 70 (HSP70) proteins have been measured using RT-qPCR. Data reported here show that Cd is a strong inducer of MT and HSP70 genes, and that BaP is a strong inducer of P-gP and AHR genes. Exposure to Cd and BaP resulted in moderate changes in antioxidant enzymes mRNA. Since the increase...
Tipo: Text
Palavras-chave: Benzo[a]pyrene; Cadmium; Metallothionein; Aryl hydrocarbon receptor; Antioxidant enzymes; RT-qPCR.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00184/29547/27873.pdf

Optimization of PMAxx pretreatment to distinguish between human norovirus with intact and altered capsids in shellfish and sewage samples
Provedor de dados: 5
Autores: Randazzo, Walter; Khezri, Mohammad; Ollivier, Joanna; Le Guyader, Soizick; Rodriguez-diaz, Jesus; Aznar, Rosa; Sanchez, Gloria.
Shellfish contamination by human noroviruses (HuNoVs) is a serious health and economic problem. Recently an ISO procedure based on RT-qPCR for the quantitative detection of HuNoVs in shellfish has been issued, but these procedures cannot discriminate between inactivated and potentially infectious viruses. The aim of the present study was to optimize a pretreatment using PMAxx to better discriminate between intact and heat-treated HuNoVs in shellfish and sewage. To this end, the optimal conditions (30 min incubation with 100 μM of PMAxx and 0.5% of Triton, and double photoactivation) were applied to mussels, oysters and cockles artificially inoculated with thermally-inactivated (99 °C for 5 min) HuNoV GI and GII. This pretreatment reduced the signal of...
Tipo: Text
Palavras-chave: Intercalating dyes; Viability PCR; Norovirus; Shellfish; Sewage; RT-qPCR.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00411/52208/52970.pdf

Identification of reference genes for expression analysis by real-time quantitative PCR in drought-stressed soybean
Provedor de dados: 86
Autores: Stolf-Moreira,Renata; Lemos,Eliana Gertrudes de Macedo; Abdelnoor,Ricardo Vilela; Beneventi,Magda Aparecida; Rolla,Amanda Alves Paiva; Pereira,Selma dos Santos; Oliveira,Maria Cristina Neves de; Nepomuceno,Alexandre Lima; Marcelino-Guimarães,Francismar Corrêa.
The objective of this work was to validate, by quantitative PCR in real time (RT-qPCR), genes to be used as reference in studies of gene expression in soybean in drought-stressed trials. Four genes commonly used in soybean were evaluated: Gmβ-actin, GmGAPDH, GmLectin and GmRNAr18S. Total RNA was extracted from six samples: three from roots in a hydroponic system with different drought intensities (0, 25, 50, 75 and 100 minutes of water stress), and three from leaves of plants grown in sand with different soil moistures (15, 5 and 2.5% gravimetric humidity). The raw cycle threshold (Ct) data were analyzed, and the efficiency of each primer was calculated for an overall analysis of the Ct range among the different samples. The GeNorm application was used to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Expression stability; RT-qPCR; Water deficit.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000100008

Variations in Tissue-Specific Expression of Adipose Differentiation-Related Protein Gene in Two Native Yunnan Chicken Breeds
Provedor de dados: 89
Autores: Zhang,B; Xiang,D; Yang,R; Yang,L; Li,J; Zhao,Z.
ABSTRACT Adipose differentiation-related protein (ADFP) is a fatty acid-binding protein that can promote the absorption of long-chain fatty acids. However, few results have been published regarding its role in Yunnan Native chicken breeds. The aim of this study was to determine ADFP gene tissue-specific expression in Piao chickens (PC) and Wuliangshan black-bone chickens (WBC) by RT-qPCR. The ontogenetic expression levels of the ADFP gene were significantly different during growth and development phases in the subcutaneous fat, liver, and pectoralis muscle of PC, and in the subcutaneous fat, liver, and pectoralis muscle of WBC (p&lt;0.05). Individual tissue-differential expression levelswere detectedon d 91 and 112 in PC, with highest levels determined in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ADFP; Carcass fatness; Piao chickens; RT-qPCR; Wuliangshan black-bone chickens.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100318

Diagnóstico serológico y molecular del Potato leafroll virus (PLRV) en tubérculos-semilla de papa en Antioquia, Colombia
Provedor de dados: 109
Autores: Mesa Medina,Mauricio; González Ramírez,Martha; Gutiérrez Sánchez,Pablo; Marín Montoya,Mauricio.
Las virosis son uno de los problemas fitosanitarios más limitantes para la producción de papa, siendo los virus Potato leafroll virus (PLRV), Potato virus Y (PVY), Potato virus S (PVS) y Potato yellow vein virus (PYVV) los que generan mayores pérdidas económicas en la región andina. Generalmente estos virus son transmitidos por insectos hemípteros y por tubérculos-semilla. En esta investigación se evaluó la presencia y distribución del PLRV en tubérculos de Solanum tuberosum variedad Diacol-Capiro y S. phureja variedad Criolla-Colombia obtenidos en Antioquia, utilizando pruebas serológicas y moleculares. Mediante DAS-ELISA se evaluaron 128 muestras consistentes en tres tipos de tejidos (piel, yemas latentes y brotes), mientras que con RT-PCR en tiempo real...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DAS-ELISA; Detección viral; Polerovirus; RT-qPCR; Solanum phureja; Solanum tuberosum.
Ano: 2016
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122016000200015

Detection and sequencing of Potato virus Y (PVY) and Potato leafroll virus (PLRV) in a volunteer plant of Solanum tuberosum L. cv. Diacol-Capiro
Provedor de dados: 109
Autores: Cárdenas,Héctor Medina; Sánchez,Pablo Gutiérrez; Montoya,Mauricio Marín.
Abstract Viral diseases are among the most limiting factors in the production of potato in Colombia and the rest of the world. The best strategy to control plant viruses consists on the use of certified seed tubers, control of arthropod vectors and the use of adequate crop management practices that reduce mechanical transmission and the presence of viral reservoirs like weeds and volunteer plants. However, the successful implementation of these practices relies on the availability of highly sensitive techniques that allow for the asymptomatic detection of viruses. In this work, we tested the performance of Next-generation sequencing (NGS) and real time RT-PCR (RT-qPCR) on a single volunteer potato plant (cv. Diacol-Capiro) growing naturally in a seed-tuber...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polerovirus; Potyvirus; RT-qPCR; High-throughput sequencing; Solanaceae.
Ano: 2017
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122017000400625

ANÁLISIS FILOGENÉTICO Y VARIABILIDAD MOLECULAR DEL Potato virus X (PVX) EN CULTIVOS DE PAPA DE ANTIOQUIA
Provedor de dados: 37
Autores: GARCÍA RUÍZ,Daniela; GUTIÉRREZ SÁNCHEZ,Pablo; MARÍN MONTOYA,Mauricio.
En este estudio se determinaron las relaciones filogenéticas y los niveles de variación de aislamientos de PVX obtenidos en tejidos foliares de plantas de Solanum tuberosum subsp. andigena var. Diacol-Capiro y S. phureja var. Criolla Colombia en Antioquia, utilizando métodos de secuenciación de nueva generación (NGS) y de Sanger. Inicialmente, se detectó el PVX mediante DAS-ELISA (Agdia-PSA10000), confirmándose su presencia en ocho de las muestras por Inmunocaptura-RT-PCR en tiempo real (IC-RT-qPCR). Los resultados de las pruebas serológicas indicaron la infección de PVX en 14,7 % y 13,3 % de las muestras de Diacol-Capiro y Criolla Colombia, respectivamente. Su identidad fue confirmada por IC-RT-qPCR, con valores de ciclo umbral (Ct) de 15,04 a 27,59 y dos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DAS-ELISA; NGS; Potexvirus; RT-qPCR; Solanum spp; Variantes.
Ano: 2016
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2016000100011

CARACTERIZACIÓN MOLECULAR DEL Potato virus V (PVV) INFECTANDO Solanum phureja MEDIANTE SECUENCIACIÓN DE NUEVA GENERACIÓN
Provedor de dados: 37
Autores: ÁLVAREZ YEPES,Daniela; GUTIÉRREZ SÁNCHEZ,Pablo Andrés; MARÍN MONTOYA,Mauricio.
Las enfermedades virales son uno de los problemas más limitantes para la producción de papa en el mundo. Uno de los materiales de papa más susceptibles a los virus corresponde a Solanum phureja; sin embargo, en Colombia son pocos los estudios adelantados sobre los agentes causales que lo afectan. En este trabajo se realizó una caracterización molecular del Potato virus V (PVV) infectando plantas de S. phureja en Antioquia, utilizando métodos de secuenciación de nueva generación (NGS), pruebas de DAS-ELISA, RT-PCR en tiempo real (RT-qPCR) y RT-PCR convencional. Los resultados indican la ocurrencia de niveles muy variables de incidencia del virus entre lotes de cultivo (6,7 % a 86 %). El PVV tiene un genoma de 9828 nt que codifica para una poliproteína de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DAS-ELISA; NGS; Papa; Potyvirus; RT-qPCR.
Ano: 2016
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2016000300007

COINFECCIÓN NATURAL DE VIRUS DE ARN EN CULTIVOS DE PAPA (Solanum tuberosum subsp. Andigena) EN ANTIOQUIA (COLOMBIA)
Provedor de dados: 37
Autores: GALLO GARCÍA,Yuliana; SIERRA MEJIA,Andrea; DONAIRE SEGARRA,Livia; ARANDA,Miguel A.; GUTIERREZ,Pablo Andrés; MARÍN MONTOYA,Mauricio.
RESUMEN Las enfermedades virales son uno de los principales problemas fitopatológicos de la papa. Con el fin de determinar los virus más prevalentes en cultivos de papa var. Diacol Capiro en el oriente Antioqueño (Colombia), se evaluó mediante RT-qPCR la presencia de diez virus de ARN (PVY, PVA, PVV, TaLMV, PVS, PLRV, PYVV, PVX, ToRSV y PMTV) en 36 muestras de tejido foliar. Los resultados indicaron la ocurrencia de cinco de los diez virus evaluados, con niveles de prevalencia de 88,9 %, 75 %, 75 %, 41,7 % y 25 % para PVY, PVX, PYVV, PLRV y PVS, respectivamente. Con fines comparativos, cuatro virus también se evaluaron mediante ELISA, siendo detectados PVS (80,5 %), PVY (55 %) y PLRV (5,5 %); mientras que PVX no fue encontrado con esta prueba. La...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ELISA; RT-qPCR; Secuenciación de nueva generación (NGS); Solanaceae; Virus de ARN.
Ano: 2019
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000300546

SECUENCIACIÓN DEL GENOMA COMPLETO DEL Potato yellow vein virus (PYVV) EN TOMATE (Solanum lycopersicum) EN COLOMBIA
Provedor de dados: 37
Autores: MUÑOZ BAENA,Laura; GUTIÉRREZ SÁNCHEZ,Pablo Andrés; MARÍN MONTOYA,Mauricio.
RESUMEN Potato yellow vein virus (PYVV), es uno de los fitopatógenos más limitantes para la producción de papa en la región de Los Andes. A pesar que se le ha detectado infectando tomate en Colombia, el conocimiento de las características biológicas de las cepas presentes en este hospedante es muy limitado. En este estudio, utilizando secuenciación masiva de nueva generación (NGS), se obtuvo la secuencia completa de los tres segmentos genómicos del PYVV en plantas de tomate en Marinilla (Antioquia) y se evalúo la utilidad de tres juegos de cebadores para su detección mediante pruebas de RT-PCR convencional y en tiempo real (RT-qPCR). El genoma de la secuencia consenso presentó tamaños de 8043 nt (ARN1), 5346 nt (ARN2) y 3896 nt (ARN3) y se identificaron...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crinivirus; RT-PCR; RT-qPCR; Secuenciación de nucleótidos de alto rendimiento; Solanaceae.
Ano: 2017
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2017000100001

Effect of osmopriming on germination and initial growth of Physalis angulata L. under salt stress and on expression of associated genes
Provedor de dados: 42
Autores: SOUZA,MANUELA O. DE; PELACANI,CLAUDINÉIA R.; WILLEMS,LEO A.J.; CASTRO,RENATO D. DE; HILHORST,HENK W.M.; LIGTERINK,WILCO.
ABSTRACT This study aimed to evaluate the effects of priming on seed germination under salt stress and gene expression in seeds and seedlings of P. angulata L. After priming for 10 days, seed germination was tested in plastic trays containing 15 ml of water (0 dS m-1 - control) or 15 ml of NaCl solution (2, 4, 6, 8, 10, 12, 14 and 16 dS m-1). Fresh and dry weight of shoots and roots of seedlings were evaluated at 0, 2, 4, 6, 8 dS m-1. Total RNA was extracted from whole seeds and seedlings followed by RT-qPCR. The target genes selected for this study were: ascorbate peroxidase (APX), glutathione-S-transferase (GST), thioredoxin (TXN), high affinity potassium transporter protein 1 (HAK1) and salt overly sensitive 1 (SOS1). At an electroconductivity of 14 dS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RT-qPCR; Germinability; Seedling growth; Salinity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200503

Expression of cell cycle inhibitors in canine prostate with proliferative inflammatory atrophy and carcinoma
Provedor de dados: 45
Autores: Faleiro,M.B.R.; Cintra,L.C.; Jesuino,R.S.A.; Damasceno,A.D.; Moura,V.M.B.D.
ABSTRACT Gene expression of CDKN1A, CDKN1B, and TP53, and immunostaining of p21, p27 and p53 were evaluated to verify the role of these cell cycle inhibitors in canine prostates with proliferative inflammatory atrophy-PIA and prostatic carcinoma-PC. Seventy samples, 15 normal, 30PIA and 25PC. Regarding number of p27 and p53 labeled cells, difference between normal and PIA and PC was observed, as well as between PIA and PC for p53. Immunostaining intensities of p21, p27 and p53 were different when comparing normal tissues to PIA and PC. Sixteen cDNA of canine prostatic FFPE tissue were subjected to RT-PCR and RT-qPCR, four normal, three PIA, and nine PC. CDKN1A mRNA was detected in four PC by RT-PCR, and it was overexpressed when compared to normal by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: P21; P27; P53; PIA; RT-qPCR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000100082

Differential expression of the genes involved in responses to water-deficit stress in peach trees cv. Chimarrita grafted onto two different rootstocks
Provedor de dados: 53
Autores: Rickes,Leticia Neutzling; Klumb,Elsa Kuhn; Benitez,Letícia Carvalho; Braga,Eugenia Jacira Bolacel; Bianchi,Valmor João.
ABSTRACT Water deficit is responsible for a negative impact on agricultural systems. Several physiological, biochemical, and molecular processes are initiated as a response to water-deficit stress. Quantifying the differential expression of the genes involved in the response to water-deficit stress plays a key role in the development of molecular strategies for engineering water-stress tolerance in plants. Therefore, the present study aims to evaluate the expression of key genes involved in the water-deficit response process in peach trees cv. Chimarrita grafted onto two different rootstocks. The experimental design was completely randomized, with four treatments that correspond to the evaluation periods: day zero (control), the fourth, seventh, and ninth...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prunus persica; Osmotic adjustment; Water stress; RT-qPCR; S6PDH; P5CS.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000100060

Rice genotypes for drought tolerance: morphological and transcriptional evaluation of auxin-related genes
Provedor de dados: 53
Autores: Madabula,Frederico Pedro; Santos,Railson Schreinert dos; Machado,Nata; Pegoraro,Camila; Kruger,Mariana Madruga; Maia,Luciano Carlos da; Sousa,Rogerio Oliveira de; Oliveira,Antonio Costa de.
ABSTRACT Drought stress affects crop quality and productivity. The challenge of increasing food availability for a growing worldwide demand relies on the development of tolerant cultivars that will need to be adapted to arid and semi-arid areas. In order to help the understanding of rice response to stress, the phenotypic response of 6 Brazilian rice cultivars and 2 different crosses between them were characterized under drought conditions. Since gene regulation is an important part of root morphological responses to stressful conditions, 4 genes related to auxin response and root modifications (OsGNOM1 CRL4, OsIAA1, OsCAND1 and OsRAA1) were evaluated. The expression of these genes was analyzed in stressed rice using public available microarray data and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oryza sativ; RT-qPCR; Root; Abiotic stress.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052016000400428

Identification of Suitable Reference Genes for Gene Expression Normalization in Jatropha curcas L During Development and Under Stress Conditions Using Real Time Quantitative PCR
Provedor de dados: 52
Autores: Rocha,Antonio José; Maranhão,Paulo Abraão; Silva,Rafaela Oliveira; Pohl,Simone; Fonteles,Cristiane S.R.
Jatropha curcas L represent a potential source of raw material for the production of biodiesel. The aim this study was to find potential candidate reference genes in J. curcas tissues. Three softwares were utilized to verify which would be the most stable reference genes in qPCR assay: GeNorm, NormFinder and BestKeeper. The most stable reference genes in developing J. curcas seeds suggested by GeNorm were GAPDH, UCP, actin. However, the best combinations of stable genes in each tissue were identified separately under stress conditions: EF1-α, PP2A2 and GAPDH in total stress, however, in SA stress, four genes were required for normalization: PP2A2, EF1-α, GAPDH and PUB. In PEG stress, four genes also were required: PP2A2, EF1-α, GAPDH and PUB, while in NaCl...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene expression; RT-qPCR; Reference genes; Jatropha curcas L.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100404

Immune response elicited by the oral administration of an intermediate strain of IBDV in chickens
Provedor de dados: 58
Autores: Carballeda,Juan Manuel; Zoth,Silvina Chimeno; Gómez,Evangelina; Lucero,María Soledad; Gravisaco,María José; Berinstein,Analía.
The immune response elicited by the oral inoculation of an intermediate strain of infectious bursal disease virus was studied in chickens. A strong over expression of IL-6, IL-8, IFNα and IFNγ was observed in bursa at 3 days post inoculation together with an increase in splenic NO2 release. An influx of T-lymphocytes was also detected.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Infectious Bursal Disease virus; Innate immunity; Avian cytokines; Flow cytometry; RT-qPCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400049

Absolute quantification of viruses by TaqMan real-time RT-PCR in grapevines
Provedor de dados: 65
Autores: Fajardo,Thor Vinícius Martins; Vanni,Marcos Fernando; Nickel,Osmar.
ABSTRACT: The absolute quantification determines the absolute amount of a targeted nucleic acid expressed as a copy number or concentration. The knowledge of virus concentrations in commercial crops possesses high relevance to ensure a reliable diagnosis. The objective of this study was to perform an absolute quantification of five viruses in infected grapevines (Vitis spp.). Different known amounts of the standard sample (cloned viral cDNA or in vitro transcribed viral RNA) were quantified by TaqMan RT-qPCR. Based on these data, standard curves were generated plotting Ct values (threshold cycle) against the log of the standard sample amount. Infected grapevine samples were evaluated to determine virus titers, which were highly variable. This result may...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RT-qPCR; GRSPaV; GVA; GVD; GLRaV-3 and -4.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000600155

IDENTIFICATION AND QUANTIFICATION OF DIFFERENTIALLY EXPRESSED GENES ASSOCIATED WITH CITRUS BLIGHT (Citrus spp.)
Provedor de dados: 64
Autores: Abreu,José Renato de; Paiva,Luciano Vilela; Rodríguez,Miguel Angel Dita; Silva,Anderson Tadeu; Henriques,Ariadne Ribeiro; Chalfun-Junior,Antonio.
Brazil is the largest citrus producer in the world, being responsible for more than 20% of its production, which is, however still low due to phytosanitary issues such as citrus blight. Citrus blight is an anomaly whose causes still have not yet been determined, therefore there are no efficient control measures to minimize the production losses with the use of resistant varieties being considered the most appropriate method. However, little is known about the genes involved in the defense response of the plants to this anomaly. Considering that many physiological alterations associated with plant stress responses are controlled at a transcriptional level, in this study we sought the identification and characterization of the gene expression products...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Differential gene expression; Subtractive hybridization; RT-qPCR.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000100032