Consulta: subjectFacets:"Real-Time PCR"
Registros recuperados: 3
Data/hora: 01/06/2024 13:38:53

Discrimination between human, pig and ruminant fecal contaminations in a river catchment by real-time PCR using host-specific markers
Provedor de dados: 5
Autores: Mieszkin, Sophie; Furet, Jean-pierre; Corthier, Gerard; Pommepuy, Monique; Le Saux, Jean-claude; Bougeard, Morgane; Hervio Heath, Dominique; Gourmelon, Michele.
The microbiological quality of coastal waters and shellfish harvesting areas in Brittany (France) can be affected by faecal pollutions from human activities and animal breeding (especially pigs and cattle). To discriminate among faecal pollution of human and animal origin, a library-independent microbial source tracking method was selected: Bacteroidales host-specific 16S rRNA gene markers by real-time Polymerase Chain Reaction (PCR). A human-specific Bacteroidales marker (Hum-1-Bac) was designed. Tested on faecal samples, the Hum-1-Bac marker showed 95 % sensitivity and 95 % specificity (n= 80). Average values (± STD) of the Hum-1-Bac marker were found to be 7.3 ± 1.4 16S rRNA gene copies per g wet faeces in human faeces samples (n=10) and 5.7 ± 1.3 log10...
Tipo: Text
Palavras-chave: Microbial Source Tracking; Host-Specific Bacteroidales Marker; 16S rRNA Gene; Real-Time PCR; Faecal Contamination.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00021/13259/10306.pdf

Expression of non-neuronal cholinergic system in maxilla of rat in vivo
Provedor de dados: 48
Autores: Guo,Jie; Wang,Lue; Xu,Haihua; Che,Xiaoxia.
BACKGROUND: Acetylcholine (ACh) is known to be a key neurotransmitter in the central and peripheral nervous systems, which is also produced in a variety of non-neuronal tissues and cell. The existence of ACh in maxilla in vivo and potential regulation role for osteogenesis need further study. RESULTS: Components of the cholinergic system (ACh, esterase, choline acetyltransferase, high-affinity choline uptake, n- and mAChRs) were determined in maxilla of rat in vivo, by means of Real-Time PCR and immunohistochemistry. Results showed RNA for CarAT, carnitine/acylcarnitine translocase member 20 (Slc25a20), VAChT, OCTN2, OCT1, OCT3, organic cation transporter member 4 (Slc22a4), AChE, BChE, nAChR subunits &amp;#945;1, &amp;#945;2, &amp;#945;3, &amp;#945;5,...
Tipo: Journal article
Palavras-chave: Non-neuronal cholinergic system; Maxilla; Real-Time PCR; Immunohistochemistry.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100066

Principles and applications of polymerase chain reaction in medical diagnostic fields: a review
Provedor de dados: 58
Autores: Valones,Marcela Agne Alves; Guimarães,Rafael Lima; Brandão,Lucas André Cavalcanti; Souza,Paulo Roberto Eleutério de; Carvalho,Alessandra de Albuquerque Tavares; Crovela,Sergio.
Recent developments in molecular methods have revolutionized the detection and characterization of microorganisms in a broad range of medical diagnostic fields, including virology, mycology, parasitology, microbiology and dentistry. Among these methods, Polymerase Chain Reaction (PCR) has generated great benefits and allowed scientific advancements. PCR is an excellent technique for the rapid detection of pathogens, including those difficult to culture. Along with conventional PCR techniques, Real-Time PCR has emerged as a technological innovation and is playing an ever-increasing role in clinical diagnostics and research laboratories. Due to its capacity to generate both qualitative and quantitative results, Real-Time PCR is considered a fast and accurate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conventional PCR; Real-Time PCR; Molecular Biology; Methods of molecular detection.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100001