Consulta: subjectFacets:"Recombination"
Registros recuperados: 12
Data/hora: 01/06/2024 08:31:53

Engineering Escherichia coli for autoinducible production of n-butanol
Provedor de dados: 69
Autores: Wang,Qinglong; ding,Yi; Liu,Li; Shi,Jiping; Sun,Junsong; Xue,Yongchang.
Background Escherichia coli does not produce n-butanol naturally, but can be butanologenic when related enzymes were expressed using inducible elements on plasmids. In this study we attempted to confer E. coli strain capability of automatic excretion of the chemical by employing a native anaerobic promoter. Also, a novel DNA kit was designed for PCR preparation of linear DNA fragments to perform strain modification. The kit is primarily composed of two mother vectors, co-transformation of linear DNAs into E. coli can simultaneously introduce two butanol synthetic operons into the chromosome and create two in-frame gene deletions at targeted native loci. Results E. coli strain Bw2V carries plasmid pCNA-PHC and pENA-TA, both utilizes native anaerobic...
Tipo: Journal article
Palavras-chave: Anaerobic promoter; Escherichia coli; Metabolic engineering; N-Butanol; Recombination.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200013

PCR-mediated recombination in development of microsatellite markers: mechanism and implications
Provedor de dados: 74
Autores: Roratto,Paula A.; Buchmann,Darine; Santos,Sandro; Bartholomei-Santos,Marlise L.
Protocols for microsatellite-enrichment libraries have been widely applied to several species in order to supply the most informative molecular markers for population and inbreeding studies. One drawback of these protocols is the ratio of designed primer pairs that fail to amplify the expected fragment, even after exhaustive optimization attempts. A possible cause of unsuccessful microsatellite primers may be that such loci are artifacts resulting from chimeric PCR products, instead of real genomic sequences. The microsatellite-enriched library constructed for Aegla longirostri (Crustacea, Decapoda, Anomura) showed that 29% of sequenced clones were chimeric products because these sequences shared one of the flanking regions around the same repeat motif but...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Chimeric PCR product; Microsatellite isolation; Recombination; Simple sequence repeats (SSR).
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100012

Estimating genomic instability mediated by Alu retroelements in breast cancer
Provedor de dados: 74
Autores: Fazza,Ana Cristina; Sabino,Flavia Cal; Setta,Nathalia de; Bordin Jr.,Newton Antonio; Silva,Eloiza Helena Tajara da; Carareto,Claudia Marcia Aparecida.
Alu-PCR is a relatively simple technique that can be used to investigate genomic instability in cancer. This technique allows identification of the loss, gain or amplification of gene sequences based on the analysis of segments between two Alu elements coupled with quantitative and qualitative analyses of the profiles obtained from tumor samples, surgical margins and blood. In this work, we used Alu-PCR to identify gene alterations in ten patients with invasive ductal breast cancer. Several deletions and insertions were identified, indicating genomic instability in the tumor and adjacent normal tissue. Although not associated with specific genes, the alterations, which involved chromosomal bands 1p36.23, 1q41, 11q14.3, 13q14.2, occurred in areas of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alu-PCR; Breast cancer; Gene insertions; Gene deletions; Invasive ductal carcinoma; Recombination.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100004

Positive selection, molecular recombination structure and phylogenetic reconstruction of members of the family Tombusviridae: implication in virus taxonomy
Provedor de dados: 74
Autores: Boulila,Moncef.
A detailed study of putative recombination events and their evolution frequency in the whole genome of the currently known members of the family Tombusviridae, comprising 79 accessions retrieved from the international databases, was carried out by using the RECCO and RDP version 3.31β algorithms. The first program allowed the detection of potential recombination sites in seven out of eight virus genera (Aureusvirus, Avenavirus, Carmovirus, Dianthovirus, Necrovirus, Panicovirus, and Tombusvirus), the second program provided the same results except for genus Dianthovirus. On the other hand, both methods failed to detect recombination breakpoints in the genome of members of genus Machlomovirus. Furthermore, based on Fisher's Exact Test of Neutrality, positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinformatics; Phylogeny; Recombination; Positive selection; Tombusviridae; Sequence; Taxonomy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400018

Wolbachia in guilds of Anastrepha fruit flies (Tephritidae) and parasitoid wasps (Braconidae)
Provedor de dados: 74
Autores: Mascarenhas,Rodrigo O; Prezotto,Leandro F; Perondini,André Luiz P; Marino,Celso Luiz; Selivon,Denise.
Abstract The endosymbiont Wolbachia is efficiently transmitted from females to their progenies, but horizontal transmission between different taxa is also known to occur. Aiming to determine if horizontal transmission might have occurred between Anastrepha fruit flies and associated braconid wasps, infection by Wolbachia was screened by amplification of a fragment of the wsp gene. Eight species of the genus Anastrepha were analyzed, from which six species of associated parasitoid wasps were recovered. The endosymbiont was found in seven Anastrepha species and in five species of braconids. The WSP Typing methodology detected eight wsp alleles belonging to Wolbachia supergroup A. Three were already known and five were new ones, among which four were found to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteria; Fruit flies; Horizontal transmission; Wsp gene; Recombination.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400600

Milk and fat production of crossbred Holstein-Gir cows (Bos taurus taurus-Bos taurus indicus) in the agreste region of the Brazilian state of Pernambuco
Provedor de dados: 74
Autores: Barbosa,Severino B.P.; Ramalho,Ricardo P.; Monardes,Humberto G.; Dias,Flávio M.; Santos,Djalma C. dos; Batista,Ângela M.V.
The productive performance of four Holstein-Gir genetic groups (1/2H, 1/4H, 5/8H, 5/8Hinter se) and the effects of non-genetic factors on production traits was evaluated using a data set of 7,951 test-day samples collected between January 1980 and December 1999 from the Arcoverde Experimental Station in the Brazilian state of Pernambuco (Empresa Pernambucana de Pesquisa Agropecuária). The statistical model included the fixed effects of calving year (1980 to 1999) and month (January to December), genetic group of the cow, age at months of calving (33 to 190) and days in milk at each test-day. Random effects were sire, cow and residual with test-days treated as repeated records within each cow within each lactation. For the four genetic groups the milk yield...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic groups; Epistasis; Genetics groups; Recombination; Repeated measures; Test-day.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300012

A model for the RecA protein of Mycoplasma synoviae
Provedor de dados: 74
Autores: Fonseca,Marbella Maria; Alarcon,Frank J.B.; Vasconcelos,Ana Tereza de; Agnez-Lima,Lucymara Fassarela.
In this work, we predict a structural model for the RecA protein from M. synoviae (MsRecA) by theoretical homology modeling and evaluate the occurrence of polymorphisms in this protein within several isolates of this species. The structural model suggested for MsRecA conserves the main domains present in MtRecA and EcRecA. The L1 and L2 regions showed six and three amino acid substitutions, respectively, which apparently do not affect the conformation and function of MsRecA. The C-terminal domain is shorter than that found in EcRecA and MtRecA, which may increase its capacity to bind dsDNA and displace SSB, compensating the absence of recombination initiation enzymes. The MS59 isolate RecA sequence showed one polymorphism which does not affect its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA repair; Recombination; RecA; Mycoplasma synoviae.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200018

A hybrid zone between Bathymodiolus mussel lineages from eastern Pacific hydrothermal vents
Provedor de dados: 5
Autores: Johnson, Shannon B.; Won, Yong-jin; Harvey, Julio B. J.; Vrijenhoek, Robert C.
Background: The inhabitants of deep-sea hydrothermal vents occupy ephemeral island-like habitats distributed sporadically along tectonic spreading-centers, back-arc basins, and volcanically active seamounts. The majority of vent taxa undergo a pelagic larval phase, and thus varying degrees of geographical subdivision, ranging from no impedance of dispersal to complete isolation, often exist among taxa that span common geomorphological boundaries. Two lineages of Bathymodiolus mussels segregate on either side of the Easter Microplate, a boundary that separates the East Pacific Rise from spreading centers connected to the Pacific-Antarctic Ridge.  Results: A recent sample from the northwest flank of the Easter Microplate contained an admixture of northern...
Tipo: Text
Palavras-chave: Bathymodiolus antarcticus n. sp; Hybridization; Recombination; Linkage disequilibrium; Deep-sea; Hydrothermal vent; Bathymodiolus thermophilus.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00185/29626/28000.pdf

Highly diverse recombining populations of Vibrio cholerae and Vibrio parahaemolyticus in French Mediterranean coastal lagoons
Provedor de dados: 5
Autores: Esteves, Kevin; Mosser, Thomas; Aujoulat, Fabien; Hervio-heath, Dominique; Monfort, Patrick; Jumas-bilak, Estelle.
Vibrio parahaemolyticus and Vibrio cholerae are ubiquitous to estuarine and marine environments. Thes two species found in Mediterranean coastal systems can induce infection in humans. Environmental isolates of V. cholerae (n = 109) and V. parahaemotycius (n = 89) sampled at different dates, stations and water salinities were investigated for virulence genes and by a mutilocus sequence-based analysis (MLSA). V. cholerae isolate were all ctxA negative and only one isolate of V. parahaemolyticus displayed trh2 gene. Most Sequence Types (ST) corresponded to uique ST isolated at one date or one station. Frequent recombination events were detected among different pathogenic species, V. parahaemotyticus, V. cholerae, Vibrio mimicus, and Vibrio metoecus....
Tipo: Text
Palavras-chave: Vibrio; Human pathogens; French coastal lagoons; Phylogeny; Recombination; Multi-locus sequence analysis; Virulence factor.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00274/38528/37039.pdf

Efficiency of pathotype mixture in screening for Downy Mildew resistance in Pearl Millet
Provedor de dados: 30
Autores: Sharma, Y.K.
Palavras-chave: Millets; Recombination; Fungi; Replication; Planting; Breeds (animals); Pathogens; Diseases; Hybrids; Application methods.
Ano: 2006
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3208

Further molecular characterization of weed-associated begomoviruses in Brazil with an emphasis on Sida spp
Provedor de dados: 87
Autores: Tavares,S.S.; Ramos-Sobrinho,R.; González-Aguilera,J.; Lima,G.S.A.; Assunção,I.P.; Zerbini,F.M.
Begomoviruses are whitefly-transmitted, single-stranded DNA viruses that are often associated with weed plants. The aim of this study was to further characterize the diversity of begomoviruses infecting weeds (mostly Sida spp.) in Brazil. Total DNA was extracted from weed samples collected in Viçosa (Minas Gerais state) and in some municipalities of Alagoas state in 2009 and 2010. Viral genomes were amplified by RCA, cloned and sequenced. A total of 26 DNA-A clones were obtained. Sequence analysis indicated the presence of 10 begomoviruses. All viral isolates from Blainvillea rhomboidea belonged to the same species, Blainvillea yellow spot virus (BlYSV ), thereby suggesting that BlYSV may be the only begomovirus present in this weed species. Four isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Geminivirus; Recombination; Blainvillea rhomboidea; Sida micrantha; Sida urens; Sida santaremnensis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582012000200009

Molecular epidemiology and evolution of avian infectious bronchitis virus
Provedor de dados: 89
Autores: Montassier,HJ.
Mutation and recombination processes are involved in the genetic and phenotypic variations of RNA viruses, leading to the emergence of new variant strains, and give rise to virus population diversity to be modeled by the host, particularly by the immune system, as occurred with infectious bronchitis virus (IBV) in chickens. The consequence is a continuous emergence of new IBV variants with regard to pathotypes, serotypes, and protectotypes. Nucleotide sequencing and subsequent genetic analysis of the S1 and N protein gene sequences provide a fast and accurate method to classify and predict IBV genotype, and a powerful instrument to monitor phylogenetic and epidemiological evolution of IBV variants. Despite the use of vaccination programmes, infectious...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken; Coronavirus; Genetic evolution; Mutation; Prophylaxis; Recombination; Variant strains.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000200003