Consulta: subjectFacets:"Representational difference analysis"
Registros recuperados: 2
Data/hora: 03/06/2024 11:24:58

Application of representational difference analysis to identify genomic differences between Bradyrhizobium elkanii and B. Japonicum species
Provedor de dados: 58
Autores: Soares,René Arderius; Passaglia,Luciane Maria Pereira.
Bradyrhizobium elkanii is successfully used in the formulation of commercial inoculants and, together with B. japonicum, it fully supplies the plant nitrogen demands. Despite the similarity between B. japonicum and B. elkanii species, several works demonstrated genetic and physiological differences between them. In this work Representational Difference Analysis (RDA) was used for genomic comparison between B. elkanii SEMIA 587, a crop inoculant strain, and B. japonicum USDA 110, a reference strain. Two hundred sequences were obtained. From these, 46 sequences belonged exclusively to the genome of B. elkanii strain, and 154 showed similarity to sequences from B. japonicum genome. From the 46 sequences with no similarity to sequences from B. japonicum, 39...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bradyrhizobium; Genome comparison; Nitrogen fixation; Representational difference analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400036

Identification of genes expressed by Cryptococcus gattii during iron deprivation
Provedor de dados: 58
Autores: Paula,Daphine Ariadne Jesus de; Silva,Lívia Kmetzsch Rosa e; Staats,Charley Christian; Vainstein,Marilene H.; Joanoni,Ana Lúcia Pinto; Nakazato,Luciano; Dutra,Valéria.
Cryptococcus neoformans and C. gattii are pathogenic yeasts that cause life-threatening diseases in humans and animals. Iron is an essential nutrient for virtually every organism as it functions as a cofactor in numerous essential enzymatic reactions. In the literature, the competition for iron between microbes and mammalian hosts during infection is well documented. In this study, we used representational difference analysis (RDA) in order to gain a better understanding of how C. gattii responds to iron starvation. A total of 15 and 29 genes were identified as having altered expression levels due to iron depletion after 3 h and 12 h, respectively. Of these, eight genes were identified in both libraries. The transcripts were related to many biological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryptococcus gattii; Iron; Representational difference analysis; Yeast.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300008