Consulta: subjectFacets:"Reverse transcription PCR"
Registros recuperados: 3
Data/hora: 01/06/2024 12:05:53

Development of a reverse transcription-PCR-DNA enzyme immunoassay for detection of "Norwalk-like" viruses and hepatitis A virus in stool and shellfish
Provedor de dados: 5
Autores: Schwab, Kellogg; Neill, Frederick; Le Guyader, Soizick; Estes, Mary; Atmar, Robert.
Outbreaks of food- and waterborne gastroenteritis are being increasingly reported throughout the world. The analysis of environmental samples by ne,ver diagnostic techniques such as reverse transcription-PCR (RT-PCR) amplification of nucleic acid has begun to identify human enteric viruses (predominantly "Norwalklike" viruses [NLVs]) as the cause of many of these outbreaks. To streamline NLV detection from environmental samples such as shellfish, we have developed an RT-PCR-oligoprobe amplification and detection method using several new procedures that enable confirmed RT-PCR amplification and product detection in 1 day. The new steps include replacing reverse transcriptase and Tag polymerase with rTth polymerase, a heat-stable enzyme that functions as...
Tipo: Text
Palavras-chave: Bivalvia; Immuoessay; DNA enzyme; Reverse transcription PCR; Detection  method; Enteric virus.
Ano: 2001
URL: https://archimer.ifremer.fr/doc/2001/publication-1267.pdf

Detection of cytotoxin-hemolysin mRNA in nonculturable populations of environmental and clinical Vibrio vulnificus strains in artificial seawater
Provedor de dados: 5
Autores: Fischer Le Saux, Marion; Hervio Heath, Dominique; Loaec, Solen; Colwell, Rita; Pommepuy, Monique.
The objective of this study was to develop a molecular detection method that better estimates the potential risk associated with the presence of Vibrio vulnificus. For that purpose, we applied seminested reverse transcription-PCR (RT-PCR) to viable but nonculturable (VBNC) populations of V. vulnificus and targeted the cytotoxin-hemolysin virulence gene vvhA. Three strains, two environmental, IF Vv10 and IF Vv18, and one clinical, C7184, were used in this study. Artificial seawater, inoculated with mid-log-phase cells, was maintained at 4degreesC. VBNC cells resulted after 3, 6, and 14 days for C7184, IF Vv18, and IF Vv10, respectively. Our data indicate that seminested RT-PCR is sensitive for the detection of vvhA mRNA in artificial seawater when...
Tipo: Text
Palavras-chave: Artificial seawater; Reverse transcription PCR; Cytotoxin Hemolysin; Molecular detection method; Vibrio vulnificus.
Ano: 2002
URL: https://archimer.ifremer.fr/doc/2002/publication-1269.pdf

Detection of human enteric viruses in shellfish collected in Tunisia
Provedor de dados: 5
Autores: Elamri, D; Aouni, M; Parnaudeau, Sylvain; Le Guyader, Soizick.
Aims: The aim of this study was to detect the main pathogenic human RNA enteric viruses able to persist in the environment such as astrovirus, enterovirus, norovirus and hepatitis A virus (HAV) in shellfish collected from two locations in northern Tunisia.   Methods and Results: Viruses were eluted from digestive tissues and concentrated by polyethylene glycol precipitation before nucleic acid extraction and purification. After checking for inhibitors, all viruses were detected by reverse transcription-polymerase chain reaction (RT-PCR) and confirmed by hybridization. Overall, 83% of the samples were found positive for at least one virus. Astrovirus was detected in 61% of the samples, norovirus in 35% and HAV in 26%. Surprisingly, only one sample was found...
Tipo: Text
Palavras-chave: Shellfish; Reverse transcription PCR; Human enteric viruses.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1881.pdf