Consulta: subjectFacets:"Rhinella schneideri"
Registros recuperados: 5
Data/hora: 14/06/2024 02:17:59

Assessment of neuropharmacological potential of low molecular weight components extracted from Rhinella schneideri toad poison
Provedor de dados: 80
Autores: Baldo,Mateus Amaral; Cunha,Alexandra Olimpio Siqueira; Godoy,Lívea Dornela; Liberato,José Luiz; Yoneda,Juliana Sakamoto; Fornari-Baldo,Elisa Correa; Ciancaglini,Pietro; Santos,Wagner Ferreira dos; Arantes,Eliane Candiani.
Abstract  Background: Studies on toad poison are relevant since they are considered a good source of toxins that act on different biological systems. Among the molecules found in the toad poison, it can be highlighted the cardiotonic heterosides, which have a known mechanism that inhibit Na+/K+-ATPase enzyme. However, these poisons have many other molecules that may have important biological actions. Therefore, this work evaluated the action of the low molecular weight components from Rhinella schneideri toad poison on Na+/K+-ATPase and their anticonvulsive and / or neurotoxic effects, in order to detect molecules with actions of biotechnological interest.  Methods: Rhinella schneideri toad (male and female) poison was collected by pressuring their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhinella schneideri; Toad poison; Bufadienolides; Seizures; Neuroprotection.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100309

First serine protease inhibitor isolated from Rhinella schneideri poison
Provedor de dados: 80
Autores: Shibao,Priscila Y T; Anjolette,Fernando A P; Lopes,Norberto P.; Arantes,Eliane C.
Background Toad secretions are a source of molecules with potential biotechnological application on a wide spectrum of diseases. Toads from theRhinella family have two kinds of poisonous glands, namely granular and mucous glands. Rhinella schneideritoads produce granular secretions that comprise a great number of molecules, including serine proteases inhibitors. Serine proteases, such as trypsin, chymotrypsin and elastase, are enzymes that have a serine amino acid into its catalytic site and can be found in a large number of vertebrate species and pathogenic microorganisms. Therefore, the present work aims to purify a serine protease inhibitor from Rhinella schneiderigranular secretions.Findings This study presents the protocol used to purify a serine...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bufadienolide; Rhinella schneideri; Serine protease inhibitor.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100501

Deep sequencing analysis of toad Rhinella schneideri skin glands and partial biochemical characterization of its cutaneous secretion
Provedor de dados: 80
Autores: Shibao,Priscila Yumi Tanaka; Cologna,Camila Takeno; Morandi-Filho,Romualdo; Wiezel,Gisele Adriano; Fujimura,Patricia Tiemi; Ueira-Vieira,Carlos; Arantes,Eliane Candiani.
Abstract  Background: Animal poisons and venoms are sources of biomolecules naturally selected. Rhinella schneideri toads are widespread in the whole Brazilian territory and they have poison glands and mucous gland. Recently, protein from toads’ secretion has gaining attention. Frog skin is widely known to present great number of host defense peptides and we hypothesize toads present them as well. In this study, we used a RNA-seq analysis from R. schneideri skin and biochemical tests with the gland secretion to unravel its protein molecules.  Methods: Total RNA from the toad skin was extracted using TRizol reagent, sequenced in duplicate using Illumina Hiseq2500 in paired end analysis. The raw reads were trimmed and de novo assembled using Trinity. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNA-seq; Rhinella schneideri; Toad secretion; Transcriptome; Illumina; Cutaneous secretion; Skin secretion; Toad protein.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100326

Biological characterization of compounds from Rhinella schneideri poison that act on the complement system
Provedor de dados: 80
Autores: Anjolette,Fernando A. P.; Leite,Flávia P.; Bordon,Karla C. F.; Azzolini,Ana Elisa C. S; Pereira,Juliana C.; Pereira-Crott,Luciana S.; Arantes,Eliane C.
Background The skin secretions of toads of the family Bufonidae contain biogenic amines, alkaloids, steroids (bufotoxins), bufodienolides (bufogenin), peptides and proteins. The poison of Rhinella schneideri, formerly classified as Bufo paracnemis, presents components that act on different biological systems, including the complement system. The aim of this study was to isolate and examine the activity ofRhinella schneideri poison (RsP) components on the complement system.Methods The components active on the complement system were purified in three chromatographic steps, using a combination of cation-exchange, anion-exchange and gel filtration chromatography. The resulting fractions were analyzed by SDS-PAGE and screened for their activity in the hemolytic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Complement system; Hemolytic activity; Neutrophil chemotaxis; Rhinella schneideri; Terminal complement complex (SC5b-9); Toad poison.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100334

Presynaptic neuromuscular action of a methanolic extract from the venom of Rhinella schneideri toad
Provedor de dados: 80
Autores: Rostelato-Ferreira,Sandro; Belo,Cháriston A Dal; Leite,Gildo B; Hyslop,Stephen; Rodrigues-Simioni,Léa.
BackgroundRhinella schneideri, previously known as Bufo paracnemis, is a common toad in many regions of Brazil. Its venom exerts important cardiovascular effects on humans and other animals. Although this toad venom has been the subject of intense investigations, little is known about its neuromuscular activity.MethodsThe neurotoxicity of a methanolic extract of R. schneideri venom was tested on mouse phrenic nerve-diaphragm (PND) preparations mounted for conventional twitch tension recording – in response to indirect stimulation – and for electrophysiological measurements.ResultsVenom extract (50 μg/mL) increased the muscle twitch tension in PND preparations but did not significantly alter the resting membrane potential values. Electrophysiological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neurotransmitter release; Ouabain; Presynaptic; Rhinella schneideri; Toad venom.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200332