Consulta: subjectFacets:"SCE"
Registros recuperados: 2
Data/hora: 10/06/2024 06:43:55

Clastogenic activity of 2-chlorodeoxyadenosine in mammalian somatic cells
Provedor de dados: 74
Autores: Antonucci,Gilmara Ausech; Takahashi,Catarina Satie.
The base analogue 2-chlorodeoxyadenosine (2-CdA) used for therapy in chronic resistant and advanced lymphoproliferative disorders, is cytotoxic for both dividing and non-dividing lymphocytes. The present work evaluated the clastogenic potential of this drug in vitro in human lymphocytes in culture and in vivo in BALB/c mice bone marrow cells. In human lymphocytes, the clastogenic effect of 2-CdA was studied in G1, S and G2 phases of the cell cycle, using three different concentrations (10, 20 and 40 mug/mL). The endpoints analyzed included mitotic index (MI), proliferation index (PI), sister chromatid exchange (SCE), and chromosomal aberration (CA). Statistical analysis by a variance (ANOVA) test showed a significant increase (p &lt; 0.05) in CA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human lymphocytes; 2-CdA; Chromosomal aberrations; SCE; Cell cycle.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200027

A critical view to explanations for the maximal value of 50 % of genetic recombination
Provedor de dados: 97
Autores: VALENZUELA,CARLOS Y; PÉREZ-ALZOLA,PATRICIA; SANTOS,MANUEL J.
The maximal value of 50 % of genetic recombination (RF = recombination fraction) between two linked gene loci has been explained by two models: (i) the restriction of crossovers to only two of the four chromatids of a tetrad (tetrad model); (ii) the limit of the occurrence of an odd number of crossovers between two loci, when the expected number of crossovers tends to infinite (odd-even model). This article shows that (i) is wrong. If tetrads were responsible for the maximal 50 % RF, sister chromatid exchanges (SCE) should display a maximal 100 % RF. In mammalian CHO cells we found that SCE yield a maximal 50 % recombination. Also, we demonstrated that the maximal 50 % RF for tetrads occurs because the sum of odd order coefficients in the binomial...
Tipo: Journal article
Palavras-chave: 50 % recombination; Tetrads; Odd-even crossovers; SCE.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2001000000015