Consulta: subjectFacets:"SELEX"
Registros recuperados: 3
Data/hora: 06/06/2024 00:34:41

First report of in vitro selection of RNA aptamers targeted to recombinant Loxosceles laeta spider toxins
Provedor de dados: 48
Autores: Sapag,Amalia; Salinas-Luypaert,Catalina; Constenla-Muñoz,Carlos.
BACKGROUND: Loxoscelism is the envenomation caused by the bite of Loxosceles spp. spiders. It entails severe necrotizing skin lesions, sometimes accompanied by systemic reactions and even death. There are no diagnostic means and treatment is mostly palliative. The main toxin, found in several isoforms in the venom, is sphingomyelinase D (SMD), a phospholipase that has been used to generate antibodies intended for medical applications. Nucleic acid aptamers are a promising alternative to antibodies. Aptamers may be isolated from a combinatorial mixture of oligonucleotides by iterative selection of those that bind to the target. In this work, two Loxosceles laeta SMD isoforms, Ll1 and Ll2, were produced in bacteria and used as targets with the aim of...
Tipo: Journal article
Palavras-chave: Antivenom; Loxosceles laeta; RNA aptamers; SELEX; SMD-Ll1; SMD-Ll2; Sphingomyelinase D.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100002

Aptamer-based therapeutics and their potential in radiopharmaceutical design
Provedor de dados: 52
Autores: Ferreira,Catia S. M.; Missailidis,Sotiris.
Aptamers, short, single stranded oligonucleotide entities, have been developed in the past 15 years against a plethora of targets and for a variety of applications. These range from inhibition of receptors and enzymes to the identification of small molecules in sensor applications, and from the development of targeted therapeutic to the design of novel diagnostic and imaging agents. Furthermore, aptamers have been designed for targets that cover a wide range of diseases, from HIV to tropical diseases, cancer and inflammation. Their easy development and flexibility of use and manipulation, offers further potential. In this paper we review their selection and consider some of the recent applications of aptamers in the design of radiopharmaceuticals for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aptamers; Radiopharmaceuticals; SELEX; Radiolabeling; Medical imaging.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600008

RNA and DNA aptamers as potential tools to prevent cell adhesion in disease
Provedor de dados: 56
Autores: Ulrich,H.; Alves,M.J.M.; Colli,W.
Recent research has shown that receptor-ligand interactions between surfaces of communicating cells are necessary prerequisites for cell proliferation, cell differentiation and immune defense. Cell-adhesion events have also been proposed for pathological conditions such as cancer growth, metastasis, and host-cell invasion by parasites such as Trypanosoma cruzi. RNA and DNA aptamers (aptus = Latin, fit) that have been selected from combinatorial nucleic acid libraries are capable of binding to cell-adhesion receptors leading to a halt in cellular processes induced by outside signals as a consequence of blockage of receptor-ligand interactions. We outline here a novel approach using RNA aptamers that bind to T. cruzi receptors and interrupt host-cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SELEX; Aptamers; Cell adhesion; Cell invasion; Trypanosoma cruzi.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300002