Consulta: subjectFacets:"SSR"
Registros recuperados: 87
Data/hora: 04/06/2024 00:37:29

Análisis de la diversidad genética de variedades nativas de chile "Poblano" por medio de microsatélites.
Provedor de dados: 32
Autores: Contreras Toledo, Aremi Rebeca.
A partir de la domesticación de chile (Capsicum annuum L.) en México, se ha generado una gran diversidad dentro de esta especie en nuestro país, derivada de la selección de los agricultores para diferentes usos, siendo el chile Poblano uno de los más importantes y el más representativo del Estado de Puebla; sin embargo, el rendimiento de chile poblano en esta región ha presentado una reducción en los últimos años, debido, en parte, a la falta de semilla mejorada que debe ser seleccionada con base en las necesidades de los propios productores, existiendo la potencial pérdida de este germoplasma. Los objetivos de este estudio fueron estimar la diversidad genética y determinar sus posibles patrones de distribución, diferenciar grupos genéticos de variedades y...
Palavras-chave: Capsicum annuum; SSR; Polimorfismo; Diferenciación genética; Polymorphism; Genetic differentiation; Maestría; Genética.
Ano: 2011
URL: http://hdl.handle.net/10521/400

Marcadores moleculares de secuencias simples repetidas en la caracterización de Lolium perenne, Lolium multiflorum, Lolium hybridum, y Dactylis glomerata
Provedor de dados: 32
Autores: Aguirre Robert, Cinthia Jimena.
En Valles Altos Centrales de México ha aumentado el cultivo de Lolium, Festuca,  Dactylis y Vicia, géneros de especies forrajeras mejor adaptadas a la zona, pero su  desventaja son recursos genéticos limitados para continuar la mejora genética de las  especies. Por tanto, el objetivo del presente estudio fue realizar una evaluación  agronómica y molecular de 8 accesiones de Lolium: Ansyl France (AF), New Zealand  (NZ), Uruguay (U), Netherland Barenza (NB), USA Manhattan II (UMII), Canada Uri (4n)  (CU4n), Australia Wimmera 62 (AW62) y France Itaque (FI); y tres de Dactylis: Canada  Hércules (CH), USA Potomac (UP) y USA Napier (UN). La estructura poblacional y  similitud genética entre accesiones se determinó con 13 loci-SSR, y se estimó la  asociación...
Tipo: Tesis
Palavras-chave: Accesiones; Loci; Caracteres morfológicos; SSR accesions; Loci; Morphological traits; SSR.
Ano: 2009
URL: http://hdl.handle.net/10521/1100

Marcadores moleculares de secuencias simples repetidas en la caracterización de Lolium perenne, Lolium multiflorum, Lolium hybridum, y Dactylis glomerata
Provedor de dados: 32
Autores: Aguirre Robert, Cinthia Jimena.
En Valles Altos Centrales de México ha aumentado el cultivo de Lolium, Festuca,  Dactylis y Vicia, géneros de especies forrajeras mejor adaptadas a la zona, pero su  desventaja son recursos genéticos limitados para continuar la mejora genética de las  especies. Por tanto, el objetivo del presente estudio fue realizar una evaluación  agronómica y molecular de 8 accesiones de Lolium: Ansyl France (AF), New Zealand  (NZ), Uruguay (U), Netherland Barenza (NB), USA Manhattan II (UMII), Canada Uri (4n)  (CU4n), Australia Wimmera 62 (AW62) y France Itaque (FI); y tres de Dactylis: Canada  Hércules (CH), USA Potomac (UP) y USA Napier (UN). La estructura poblacional y  similitud genética entre accesiones se determinó con 13 loci-SSR, y se estimó la  asociación...
Tipo: Tesis
Palavras-chave: Accesiones; Loci; Caracteres morfológicos; SSR; Maestría; Ganadería; Accesions; Loci; Morphological traits.
Ano: 2009
URL: http://hdl.handle.net/10521/1448

Análisis de la diversidad genética de variedades nativas de chile "Poblano" por medio de microsatélites.
Provedor de dados: 32
Autores: Contreras Toledo, Aremi Rebeca.
A partir de la domesticación de chile (Capsicum annuum L.) en México, se ha generado una gran diversidad dentro de esta especie en nuestro país, derivada de la selección de los agricultores para diferentes usos, siendo el chile Poblano uno de los más importantes y el más representativo del Estado de Puebla; sin embargo, el rendimiento de chile poblano en esta región ha presentado una reducción en los últimos años, debido, en parte, a la falta de semilla mejorada que debe ser seleccionada con base en las necesidades de los propios productores, existiendo la potencial pérdida de este germoplasma. Los objetivos de este estudio fueron estimar la diversidad genética y determinar sus posibles patrones de distribución, diferenciar grupos genéticos de variedades y...
Palavras-chave: Capsicum annuum; SSR; Polimorfismo; Diferenciación genética; Polymorphism; Genetic differentiation; Maestría; Genética.
Ano: 2011
URL: http://hdl.handle.net/10521/400

Evaluation of cassava plants generated by somatic embryogenesis at different stages of development using molecular markers
Provedor de dados: 69
Autores: Osorio,Mayra; Gámez,Elizabeth; Molina,Sandy; Infante,Diógenes.
Background: Cassava (Manihot esculenta Crantz) is a crop that is high in carbohydrates in the roots and in protein in the leaves, important for both human consumption and animal feed, and also has a significant industrial use for its starches. In this study we evaluated the genetic variability with molecular markers in different stages in micropropagated plants from somatic embryos of Venezuelan native clone 56. Results: Three markers were used: ISTR, AFLP and SSR, finding that ISTR showed the highest polymorphism among individuals tested. With AFLP a high similarity between the evaluated individuals was observed and with SSR total monomorphism was seen. Using cluster analysis it was found that individuals from an embryo labeled as fasciated at the...
Tipo: Journal article
Palavras-chave: AFLP; Genetic stability; ISTR; SSR; UPGMA.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400003

Assessment of genetic diversity in Venezuelan rice cultivars using simple sequence repeats markers
Provedor de dados: 69
Autores: Ghneim Herrera,Thaura; Posso Duque,Duina; Pérez Almeida,Iris; Torrealba Núñez,Gelis; Pieters,Alejandro J; Martinez,César P; Tohme,Joe M.
In Venezuela, pedigree analyses indicate that the rice varieties currently under cultivation are closely related. Effective breeding programs, based on knowledge of the genetic diversity of cultivars, are needed to broaden the genetic bases of rice germplasm in the country. In this study, we used a set of 48 simple-sequence-repeat (SSR) markers to assess the genetic diversity of 11 Venezuelan rice cultivars, released by the National Rice Breeding Program between 1978 and 2007. A total of 203 alleles were detected, the number of alleles (NA) per marker ranged from 2 to 9, with an average of 4.23. The average genic diversity (H) over all SSR loci for the 18 genotypes was 0.524, ranging from 0.105 to 0.815. Positive correlations were found between H at each...
Tipo: Journal article
Palavras-chave: DNA fingerprinting; Genetic diversity; Rice; SSR.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500003

Identification of groundnut (Arachis hypogaea) SSR markers suitable for multiple resistance traits QTL mapping in African germplasm
Provedor de dados: 69
Autores: Ncube Kanyika,Busisiwe T; Lungu,Davies; Mweetwa,Alice M; Kaimoyo,Evans; Njung'e,Vincent M; Monyo,Emmanuel S; Siambi,Moses; He,Guohao; Prakash,Channapata S; Zhao,Yongli; de Villiers,Santie M.
Background This study aimed to identify and select informative Simple Sequence Repeat (SSR) markers that may be linked to resistance to important groundnut diseases such as Early Leaf Spot, Groundnut Rosette Disease, rust and aflatoxin contamination. To this end, 799 markers were screened across 16 farmer preferred and other cultivated African groundnut varieties that are routinely used in groundnut improvement, some with known resistance traits. Results The SSR markers amplified 817 loci and were graded on a scale of 1 to 4 according to successful amplification and ease of scoring of amplified alleles. Of these, 376 markers exhibited Polymorphic Information Content (PIC) values ranging from 0.06 to 0.86, with 1476 alleles detected at an average of 3.7...
Tipo: Journal article
Palavras-chave: Arachis; African varieties; Disease; SSR.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200001

Genetic mapping of EST-SSR, SSR and InDel to improve saturation of genomic regions in a previously developed sunflower map
Provedor de dados: 69
Autores: Talia,Paola; Nishinakamasu,Verónica; Hopp,Horacio Esteban; Heinz,Ruth Amelia; Paniego,Norma.
In order to saturate a sunflower genetic map and facilitate marker-assisted selection (MAS) breeding for stress response, it is necessary to enhance map saturation with molecular markers localized in linkage groups associated to genomic regions involved in these traits. This work describes the identification and characterization of 1,134 simple sequence repeat (SSR) containing expressed sequence tags (EST) from unigenes available databases. Twelve of these functional markers as well as 41 public SSR markers were successfully localized in linkage groups, thus contributing to the saturation of specific regions on a reference genetic-linkage-map derived from recombinant inbred lines (RIL) mapping population from the cross between PAC2 x RHA266 lines. The...
Tipo: Journal article
Palavras-chave: EST-SSR; InDels; Linkage map; SSR; Sunflower.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600007

Development of cDNA-derived SSR markers and their efficiency in diversity assessment of Cymbidium accessions
Provedor de dados: 69
Autores: Moe,Kyaw Thu; Hong,Woo-Ju; Kwon,Soon-Wook; Park,Yong-Jin.
Cymbidium spp. are popular &amp;#64258;owering plants. Assessment of the genetic diversity in cultivated Cymbidium facilitates conservation of germplasm and subsequent cultivar improvement. Thus, it is important to develop more efficient polymorphic DNA markers. Although more motifs (403) were identified and more primers (206) were designed in the genomic library compared to the cDNA library, a larger number of successful primers were obtained from the cDNA library (59.9%) than from genomic DNA library (51.1%). However, higher PIC and gene diversity were identified in genomic SSRs. The average allele number per locus was also higher in genomic SSRs (7.3) than EST-SSRs (5.2), among the 24 evaluated Cymbidium accessions. AT/TA was comparatively high in...
Tipo: Journal article
Palavras-chave: CDNA library; Cymbidium; Enriched library; Molecular diversity; SSR.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200004

Identification of SSR markers using soybean (Glycine max) ESTs from globular stage embryos
Provedor de dados: 69
Autores: Li,Ai Qin; Zhao,Chuan Zhi; Wang,Xing Jun; Liu,Zhan Ji; Zhang,Li Feng; Song,Guo Qi; Yin,Juan; Li,Chang Sheng; Xia,Han; Bi,Yu Ping.
Microsatellites, or simple sequence repeats (SSRs), in expressed sequence tags (ESTs) provide an opportunity for low cost SSR development. We looked for EST-SSRs in 403,511 ESTs (generated by 454 sequencing and representing 70,654 contigs and 52,082 singletons) from soybean globular stage embryos. Among 122,736 unique ESTs, 3,729 contained one or more SSRs. In total, 3,989 SSRs were identified including 304 mono, 1,374 di, 2,208 tri, 70 tetra, 13 penta and 20 hexanucleotide SSRs. Thirty three EST-SSRs were selected for primer design and polymorphism analysis using twenty soybean cultivars and one wild-type soybean. Successful amplification was obtained using 21 of these primer pairs, 11 of which detected polymorphisms in these soybean cultivars. These...
Tipo: Journal article
Palavras-chave: 454 sequencing; EST; Glycine max; Polymorphism; SSR.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500006

Comparison of whole-genome amplifications for microsatellite genotyping of Rotylenchulus reniformis
Provedor de dados: 69
Autores: Arias,Renée S; Stetina,Salliana R; Scheffler,Brian E.
Currently, a large number of microsatellites are available for Rotylenchulus reniformis (reniform nematode); however, two barriers exist for genotyping samples from different geographical areas. The limited amount of nucleic acids obtained from single nematodes which would require their multiplication to obtain enough DNA for testing; and the strictly regulated transport of live samples and multiplication in greenhouse for being a plant pathogen. Whole-genome amplification (WGA) of samples consisting of one and five dead gravid females with their associated egg masses was successfully performed on disrupted tissue using three commercial kits. DNA yield after WGA ranged from 0.5 to 8 Âµg and was used to test 96 microsatellite markers we previously developed...
Tipo: Journal article
Palavras-chave: Genotyping; Reniform nematode; SSR; STR; WGA.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300014

Microsatellite markers in candidate genes for wood properties and its application in functional diversity assessment in Eucalyptus globulus
Provedor de dados: 69
Autores: Acuña,Cintia V; Villalba,Pamela V; García,Martín; Pathauer,Pablo; Hopp,H. Esteban; Marcucci Poltri,Susana N.
Background: Functional genetic markers have important implications for genetic analysis by providing direct estimation of functional diversity. Although high throughput sequencing techniques for functional diversity analysis are being developed nowadays, the use of already well established variable markers present in candidate genes is still an interesting alternative for mapping purposes and functional diversity studies. SSR markers are routinely used in most plant and animal breeding programs for many species including Eucalyptus. SSR markers derived from candidate genes (SSR-CG) can be used effectively in co-segregation studies and marker-assisted diversity management. Results: In the present study, eight new non reported SSRs were identified in seven...
Tipo: Journal article
Palavras-chave: Functional markers; Genetic diversity; Lignin pathway; SSR; Wood density.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200002

Genetic diversity in two Italian almond collections
Provedor de dados: 69
Autores: Rigoldi,Maria Pia; Rapposelli,Emma; De Giorgio,Donato; Resta,Paolo; Porceddu,Andrea.
Background Sweet-seeded domesticated almonds were brought to the Mediterranean Basin from central Asia about 4000 years ago. In Italy, most of the almonds produced are cultivated in the southern part of the country. Local populations of the tree in Sardinia are largely seed-derived and mostly self-incompatible, so have developed extensive genetic diversity. The need to protect biodiversity has prompted a revived interest in local genetic materials in almond. Two Italian collections have been established, one in Sardinia and the other in Apulia. These collections were the focus of the present evaluation of genetic diversity. Results Eleven SSRs (microsatellites) were used for fingerprinting. The Sardinian germplasm was highly polymorphic, revealing a mean...
Tipo: Journal article
Palavras-chave: Biodiversity; Genetic structure; Prunus amygdalus; SSR.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100008

Agronomic and molecular analyses for the characterisation of accessions in Tunisian olive germplasm collections
Provedor de dados: 69
Autores: Taamalli,Wael; Geuna,Filippo; Banfi,Riccardo; Bassi,Daniele; Daoud,Douja; Zarrouk,Mokhtar.
In this study, a total of 14 agronomic traits, five AFLP primer combinations and ten SSR loci were used to describe and to classify a group of Tunisian olive genotypes into groups based on molecular profiles and agronomic traits. The analysis of variance of the agronomical data revealed significant differences among accessions for all measured traits. The mean phenotypic dissimilarity (0.34 with a range of 0.08-0.6) was low in comparison to dissimilarity calculated using AFLP (0.50 with a range of 0.16-0.70) and SSR markers (0.76 with a range 0.35-0.94). The correlation between the agronomical dissimilarity matrix and the matrices of genetic dissimilarity based on SSR and AFLP markers was very weak: 0.156 (p = 0.05) and 0.185 (p = 0.05), respectively. The...
Tipo: Journal article
Palavras-chave: Agronomic traits; AFLP; Olea europaea; SSR.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500002

Identification of a minimal microsatellite marker panel for the fingerprinting of peach and nectarine cultivars
Provedor de dados: 69
Autores: Rojas,Gabriela; Méndez,Marco A; Muñoz,Carlos; Lemus,Gamalier; Hinrichsen,Patricio.
The genetic characterization of 117 peach and nectarine cultivars (Prunus persica (L.) Batsch) using microsatellite (SSR) markers is presented. Analyzed genotypes include the complete list of cultivars under intellectual property (IP) protection in Chile. One hundred and two out of the 117 cultivars under study could be identified using only 7 SSRs. Other 5 cultivars were differentiated using 3 additional markers, but 5 pairs of genotypes were not differentiable. The average expected heterozygosity for the set of markers was 0.55, ranging from 0.28 in BPPCT-008 to 0.81 in CPPCT-022, with an F value of 0.37. A Neighbor-Joining dendrogram showed that, with few exceptions, peaches and nectarines clustered separately. These results are the basis for the...
Tipo: Journal article
Palavras-chave: Cultivar identification; Fingerprinting; Prunus persica; Simple sequence repeats; SSR.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500004

Quantitative trait loci mapping of pubescence density and flowering time of insect-resistant soybean (Glycine max L. Merr.)
Provedor de dados: 74
Autores: Komatsu,Kunihiko; Okuda,Shiori; Takahashi,Masakazu; Matsunaga,Ryoichi; Nakazawa,Yoshinori.
Analysis of antibiosis resistance to common cutworm (Spodoptera litura Fabricius) in soybean (Glycine max (L.) Merr.) has progressed significantly, but the immediate cause remains unknown. We performed quantitative trait loci (QTL) analysis of pubescence density and plant development stage because these factors are assumed to be the immediate cause of resistance to cutworm. The QTLs for pubescence appeared to be identical to the previously detected the Pd1 and Ps loci controlling pubescence density. We found no candidate loci for flowering time QTLs, although one could be identical to the gene governing the long-juvenile trait or to the E6 loci controlling maturity. None of the QTLs overlapped with the QTLs for antibiosis resistance.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glycine max; Hair; Maturity; Pest resistance; QTL; SSR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400022

Genetic diversity in Brazilian sweet potato (Ipomoea batatas (L.) Lam., Solanales, Convolvulaceae) landraces assessed with microsatellite markers
Provedor de dados: 74
Autores: Veasey,Elizabeth Ann; Borges,Aline; Rosa,Mariana Silva; Queiroz-Silva,Jurema R.; Bressan,Eduardo de Andrade; Peroni,Nivaldo.
We used simple sequence repeat (SSR) markers to investigate the genetic diversity of 78 sweet potato (Ipomoea batatas) accessions (58 landraces and 20 putative clones) from traditional agricultural households from 19 local communities in the Vale do Ribeira, São Paulo, Brazil. Eight SSR loci were assessed using 6% (w/v) polyacrylamide gels stained with silver nitrate and the accessions genotyped considering the presence or absence of bands. The results were subjected to analysis of molecular variance (AMOVA), and cluster and principal coordinate analyses. Spatial structure was assessed using Mantel's test to compare genetic and geographic distances. Each primer pair generated between three and ten clearly scorable polymorphic fragments. Cluster analyses...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Landraces; SSR; Traditional agriculture; Vale do Ribeira.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400020

Tandem repeat distribution of gene transcripts in three plant families
Provedor de dados: 74
Autores: Maia,Luciano Carlos da; Souza,Velci Queiróz de; Kopp,Mauricio Marini; Carvalho,Fernando Irajá Félix de; Oliveira,Antonio Costa de.
Tandem repeats (microsatellites or SSRs) are molecular markers with great potential for plant genetic studies. Modern strategies include the transfer of these markers among widely studied and orphan species. In silico analyses allow for studying distribution patterns of microsatellites and predicting which motifs would be more amenable to interspecies transfer. Transcribed sequences (Unigene) from ten species of three plant families were surveyed for the occurrence of micro and minisatellites. Transcripts from different species displayed different rates of tandem repeat occurrence, ranging from 1.47% to 11.28%. Both similar and different patterns were found within and among plant families. The results also indicate a lack of association between genome size...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; EST; Comparative genomics; Molecular markers.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400025

In silico analysis of polymorphic microsatellites in penaeid shrimp and construction of a free-access database
Provedor de dados: 74
Autores: Freitas,Patrícia Domingues de; Pinheiro,Allysson Pontes; Silva,Thiago Buosi; Galetti Junior,Pedro Manoel.
We performed an in silico analysis of all microsatellites so far described for penaeid shrimp and for which the polymorphic behavior has previously been analyzed. The objective of the study was to evaluate the structural characteristics of these microsatellites and identifying patterns which allow the characterization of the nature of these sequences in the penaeid genome. All data were compiled in a free-access database specially constructed for this study. Three hundred non-mononucleotide polymorphic microsatellite loci described for 12 shrimp species belonging to the family Penaeidae were analyzed and simple and compound microsatellites with di-, tri-, tetra-, penta- and hexanucleotide motifs were found. Dinucleotides and trinucleotides were the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crustacea; Microsatellite structure; Penaeidae; SSR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600027

SSR and AFLP based genetic diversity of soybean germplasm differing in photoperiod sensitivity
Provedor de dados: 74
Autores: Singh,Ram K.; Bhatia,Virendra S.; Bhat,K.V.; Mohapatra,Trilochan; Singh,Nagendra K.; Bansal,Kailash C.; Koundal,K.R.
Forty-four soybean genotypes with different photoperiod response were selected after screening of 1000 soybean accessions under artificial condition and were profiled using 40 SSR and 5 AFLP primer pairs. The average polymorphism information content (PIC) for SSR and AFLP marker systems was 0.507 and 0.120, respectively. Clustering of genotypes was done using UPGMA method for SSR and AFLP and correlation was 0.337 and 0.504, respectively. Mantel's correlation coefficients between Jaccard's similarity coefficient and the cophenetic values were fairly high in both the marker systems (SSR = 0.924; AFLP = 0.958) indicating very good fit for the clustering pattern. UPGMA based cluster analysis classified soybean genotypes into four major groups with fairly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photoperiod response; SSR; AFLP; Genetic diversity; Soybean.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200019

The genetic structure and mating system of Acrocomia aculeata (Arecaceae)
Provedor de dados: 74
Autores: Abreu,Aluana Gonçalves; Priolli,Regina Helena Geribello; Azevedo-Filho,Joaquim Adelino; Nucci,Stella Maris; Zucchi,Maria Imaculada; Coelho,Ricardo Marques; Colombo,Carlos Augusto.
Acrocomia aculeata is a perennial, fruit-producing palm tree, native to tropical forests. Its fruits have spurred interest because of their significant potential for use in the cosmetic industry and as feedstock for biofuel. In the present study, the genetic structure and mating system in Acrocomia aculeata were analyzed, using eight nuclear microsatellite loci and samples from São Paulo and Minas Gerais states, Brazil. By means of Bayesian analysis, these populations were clustered into two or three groups. A high multilocus outcrossing rate suggests that outcrosses were predominant, although a certain degree of biparental inbreeding also occurred. Thus, although monoecious and self-compatible, there is every indication that A. aculeata bears a mixed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; Mating system.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100016

Mining plant genome browsers as a means for efficient connection of physical, genetic and cytogenetic mapping: an example using soybean
Provedor de dados: 74
Autores: Belarmino,Luis C.; Oliveira,Ana R. da S.; Brasileiro-Vida,Ana C.; Bortoleti,Kyria C. de A.; Bezerra-Neto,João Pacífico; Abdelnoor,Ricardo V.; Benko-Iseppon,Ana M.
Physical maps are important tools to uncover general chromosome structure as well as to compare different plant lineages and species, helping to elucidate genome structure, evolution and possibilities regarding synteny and colinearity. The increasing production of sequence data has opened an opportunity to link information from mapping studies to the underlying sequences. Genome browsers are invaluable platforms that provide access to these sequences, including tools for genome analysis, allowing the integration of multivariate information, and thus aiding to explain the emergence of complex genomes. The present work presents a tutorial regarding the use of genome browsers to develop targeted physical mapping, providing also a general overview and examples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene families; FISH; BAC; SSR; Aquaporin; Bioinformatics.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200015

A novel statistical method for assessing SSR variation in autotetraploid alfalfa (Medicago sativa L.)
Provedor de dados: 74
Autores: Liu,Zhi-Peng; Liu,Gong-She; Yang,Qing-Chuan.
The level of variation of simple sequence repeat (SSR) markers in cultivated alfalfa from American, Australian and Chinese sources was evaluated using a novel autotetraploid statistical method to calculate the effective number of alleles, the allele frequencies and heterozygosity. We used 19 SSR primers to screen seven polymorphic SSR loci in 320 plants from eight populations. The genetic distance and phylogenetic analysis (DISPAN) program was used to calculate the inter- and intra-population genetic relationships using the conventional binary absence/presence (0/1) method and our novel autotetraploid method. The autotetraploid method resulted in significantly higher heterozygosity (p &lt; 0.01), average effective number (p &lt; 0.01) and lower standard...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Autotetraploid; Genetic variation; Medicago sativa L.; SSR; Statistical genetics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300015

Development of microsatellite markers for identifying Brazilian Coffea arabica varieties
Provedor de dados: 74
Autores: Vieira,Elisa S.N.; Von Pinho,Édila V. de R.; Carvalho,Maria G.G.; Esselink,Danny G.; Vosman,Ben.
Microsatellite markers, also known as SSRs (Simple Sequence Repeats), have proved to be excellent tools for identifying variety and determining genetic relationships. A set of 127 SSR markers was used to analyze genetic similarity in twenty five Coffea arabica varieties. These were composed of nineteen commercially important Brazilians and six interspecific hybrids of Coffea arabica, Coffea canephora and Coffea liberica. The set used comprised 52 newly developed SSR markers derived from microsatellite enriched libraries, 56 designed on the basis of coffee SSR sequences available from public databases, 6 already published, and 13 universal chloroplast microsatellite markers. Only 22 were polymorphic, these detecting 2-7 alleles per marker, an average of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; Coffee; Genetic similarity; Molecular marker.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300022

Cross-species microsatellite amplification in South American Caimans (Caiman spp and Paleosuchus palpebrosus)
Provedor de dados: 74
Autores: Zucoloto,Rodrigo Barban; Villela,Priscilla Marqui Schimidt; Verdade,Luciano Martins; Coutinho,Luiz Lehmann.
Microsatellite DNA markers have been used to assess genetic diversity and to study ecological behavioral characteristics in animals. Although these markers are powerful tools, their development is labor intensive and costly. Thus, before new markers are developed it is important to prospect the use of markers from related species. In the present study we investigated the possibility of using microsatellite markers developed for Alligator mississipiensis and Caiman latirostris in South American crocodilians. Our results demonstrate the use of microsatellite markers for Paleosuchus palpebrosus, Caiman crocodilus and Caiman yacare.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; STR; Primers; Crocodilians; Alligatorinae.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100015

Satellyptus: analysis and database of microsatellites from ESTs of Eucalyptus
Provedor de dados: 74
Autores: Ceresini,Paulo Cezar; Silva,Cristina Lacerda Soares Petrarolha; Missio,Robson Fernando; Souza,Elaine Costa; Fischer,Carlos Norberto; Guillherme,Ivan Rizzo; Gregorio,Ivo; Silva,Eloiza Helena Tajara da; Cicarelli,Regina Maria Barreto; Silva,Marco Túlio Alves da; Garcia,José Fernando; Avelar,Gustavo Arbex; Porto Neto,Laercio Ribeiro; Marçon,André Ricardo; Bacci Junior,Maurício; Marini,Danyelle Cristine.
The main goal of our research was to search for SSRs in the Eucalyptus EST FORESTs database (using a software for mining SSR-motifs). With this objective, we created a database for cataloging Eucalyptus EST-derived SSRs, and developed a bioinformatics tool, named Satellyptus, for finding and analyzing microsatellites in the Eucalyptus EST database. The search for microsatellites in the FORESTs database containing 71,115 Eucalyptus EST sequences (52.09 Mb) revealed 20,530 SSRs in 15,621 ESTs. The SSR abundance detected on the Eucalyptus ESTs database (29% or one microsatellite every four sequences) is considered very high for plants. Amongst the categories of SSR motifs, the dimeric (37%) and trimeric ones (33%) predominated. The AG/CT motif was the most...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioinformatics tool; Genetic markers; Gene ontology; Small sequence repeats; SSR.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400014

Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers
Provedor de dados: 74
Autores: Zucchi,Maria Imaculada; Brondani,Rosana Pereira Vianello; Pinheiro,José Baldin; Chaves,Lázaro José; Coelho,Alexandre Siqueira Guedes; Vencovsky,Roland.
The ''cagaita tree'' (Eugenia dysenterica) is a plant found widespread in the Brazilian Cerrado. Its fruit is used for popular consumption and for industrial purposes. This study opens a new perspective for the generation of population genetic data and parameters estimates for devising sound collection and conservation procedures for Eugenia dysenterica. A battery of 356 primer pairs developed for Eucalyptus spp. was tested on the ''cagaita tree''. Only 10 primer pairs were found to be transferable between the two species. Using a polyacrilamide gel, an average of 10.4 alleles per locus was detected, in a sample of 116 individuals from 10 natural ''cagaita tree'' populations. Seven polymorphic loci allowed estimation of genetic parameters, including...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; Myrtaceae; Cerrado; Tropical tree; Genetic diversity and transferability.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400008

High levels of genetic differentiation and selfing in the Brazilian cerrado fruit tree Dipteryx alata Vog. (Fabaceae)
Provedor de dados: 74
Autores: Tarazi,Roberto; Moreno,Maria Andréia; Gandara,Flávio Bertin; Ferraz,Elza Martins; Moraes,Mário Luiz Teixeira; Vinson,Christina Cleo; Ciampi,Ana Yamaguishi; Vencovsky,Roland; Kageyama,Paulo Yoshio.
Dipteryx alata is a native fruit tree species of the cerrado (Brazilian savanna) that has great economic potential because of its multiple uses. Knowledge of how the genetic variability of this species is organized within and among populations would be useful for genetic conservation and breeding programs. We used nine simple sequence repeat (SSR) primers developed for Dipteryx odorata to evaluate the genetic structure of three populations of D. alata located in central Brazil based on a leaf sample analysis from 101 adults. The outcrossing rate was evaluated using 300 open-pollinated offspring from 25 seed-trees. Pollen dispersal was measured by parentage analysis. We used spatial genetic structure (SGS) to test the minimal distance for harvesting seeds...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dipteryx alata; Genetic structure; Mating system; SSR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100015

Genetic diversity in populations of African mahogany (Khaya grandioliola C. DC.) introduced in Brazil
Provedor de dados: 74
Autores: Soares,Sabrina Delgado; Bandeira,Ludmila Ferreira; Ribeiro,Stela Barros; Telles,Mariana Pires de Campos; Silva,João Augusto da; Borges,Canrobert Tormin; Coelho,Alexandre Siqueira Guedes; Novaes,Evandro.
Abstract Given its high-valued wood, the African mahogany (Khaya grandifoliola) has been envisaged as a renewable source of tropical hardwoods in Brazil. However, there are concerns about the hypothesized low diversity among the few K. grandifoliola germplasm sources introduced in the country. Using eight microsatellite markers, we evaluated the genetic diversity and divergence among 53 superior trees selected from three provenances of K. grandifoliola located in the state of Para. These populations are among the oldest plantations (&gt;15 years) in Brazil and, therefore, the country's main seed sources. The average number of alleles per locus was 5.9, expected heterozygosity was moderate (^=0.56) and lower than the high observed heterozygosity (HO=0.74)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; SSR; Genetic structure; Genetic divergence; Hardwood.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400301

Intraspecific differentiation of Chilean isolates of the entomopathogenic fungi Metarhizium anisopliae var. anisopliae as revealed by RAPD, SSR and ITS markers
Provedor de dados: 74
Autores: Becerra Velásquez,Viviana; Paredes Cárcamo,Mario; Rojo Meriño,Carmen; France Iglesias,Andrés; Franco Durán,Jorge.
The genus Metarhizium consists of a diverse group of asexual entomopathogenic fungi, which have a wide geographical distribution. The Chilean National Agricultural Research Institute (Instituto de Investigaciones Agropecuarias - INIA, Quilamapu Chile) has collected about 350 isolates of Metarhizium anisopliae var. anisopliae from central and southern Chile. These isolates have been partially characterized using morphological traits such as conidia size and shape, colony color, growth pattern and the efficiency of the isolates in controlling specific pests. However, further characterization with molecular markers could detect differences in DNA which could help to better understand the genetic diversity and structure of Chilean populations of this fungus....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metarhizium; Genetic diversity; RAPD; SSR; ITS markers.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100017

Polymorphic heterologous microsatellite loci for population genetics studies of the white-faced ibis Plegadis chihi (Vieillot, 1817) (Pelecaniformes, Threskiornithidae)
Provedor de dados: 74
Autores: Souza,Andiara Silos Moraes de Castro e; Miño,Carolina Isabel; Lama,Silvia Nassif Del.
We screened 44 heterologous microsatellites isolated in species of the families Threskiornithidae, Ciconiidae and Ardeidae for their use in a migratory waterbird, the white-faced ibis Plegadis chihi (Vieillot, 1817) (Threskiornithidae). Of the screened loci, 57% amplified successfully and 24% were polymorphic. In two breeding colonies from southern Brazil (N = 131) we detected 32 alleles (2-10 alleles/ locus). Average He over all loci and colonies was 0.55, and the combined probability of excluding false parents, 98%. There was no departure from HWE in any loci or population. Eru6 and Eru4 loci were in non-random association in the Alvorada colony, and NnNF5 and Eru5 in both populations. AMOVA analysis indicated that most of the genetic diversity was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cross-species amplification; Genetic diversity; Molecular markers; White-faced ibis; SSR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100010

Chromosomal localization of microsatellite loci in Drosophila mediopunctata
Provedor de dados: 74
Autores: Cavasini,Renato; Batista,Marcos Roberto Dias; Klaczko,Louis Bernard.
Drosophila mediopunctata has been used as a model organism for genetics and evolutionary studies in the last three decades. A linkage map with 48 microsatellite loci recently published for this species showed five syntenic groups, which had their homology determined to Drosophila melanogaster chromosomes. Then, by inference, each of the groups was associated with one of the five major chromosomes of D. mediopunctata. Our objective was to carry out a genetic (chromosomal) analysis to increase the number of available loci with known chromosomal location. We made a simultaneous analysis of visible mutant phenotypes and microsatellite genotypes in a backcross of a standard strain and a mutant strain, which had each major autosome marked. Hence, we could...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Tripunctata; SSR; Genetic chromosomal analysis; BLAST search; Muller’s elements.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100055

Population structures of Brazilian Tall coconut (Cocos nucifera L.) by microsatellite markers
Provedor de dados: 74
Autores: Ribeiro,Francisco E.; Baudouin,Luc; Lebrun,Patricia; Chaves,Lázaro J.; Brondani,Claudio; Zucchi,Maria I.; Vencovsky,Roland.
Coconut palms of the Tall group were introduced to Brazil from the Cape Verde Islands in 1553. The present study sought to evaluate the genetic diversity among and within Brazilian Tall coconut populations. Samples were collected of 195 trees from 10 populations. Genetic diversity was accessed by investigating 13 simple sequence repeats (SSR) loci. This provided a total of 68 alleles, ranging from 2 to 13 alleles per locus, with an average of 5.23. The mean values of gene diversity (He) and observed heterozygosity (Ho) were 0.459 and 0.443, respectively. The genetic differentiation among populations was estimated at θ = 0.1600 and the estimated apparent outcrossing rate was t a = 0.92. Estimates of genetic distances between the populations varied from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coconut; Genetic diversity; Genetic variability; Molecular markers; SSR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400016

Molecular characterization of watermelon cultivars using microsatellite markers
Provedor de dados: 75
Autores: Gama,Renata Natália CS; Santos,Carlos Antônio F; Dias,Rita de Cássia S; Souza,Flávio F.
Allelic patterns and genetic similarity among 17 watermelon cultivars were established using microsatellite markers. For visualization of the genetic similarity, the dendrogram UPGMA (Unweighted Pair Group Method with Arithmetic Means) was generated by the similarity matrix of the Jacquard coefficient, based on 34 alleles of ten microsatellite loci. Total DNA was extracted by the CTAB 2x method and PCR (Polymerase Chain Reaction) products were analyzed in denaturing polyacrylamide 6% gels, stained with silver nitrate. The number of base pairs was estimated by the method of inverse mobility, based on known size product regression. Similarity ranged from 34 to 100%, reflecting high genetic variability. Analyzed loci were not enough to distinguish all 17...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrullus lanatus; Genetic divergence; SSR; Plant variety protection.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000400003

DNA fingerprinting of water yam (Dioscorea alata) cultivars in Brazil based on microsatellite markers
Provedor de dados: 75
Autores: Siqueira,Marcos VBM; Dequigiovanni,Gabriel; Corazon-Guivin,Mike A; Feltran,José C; Veasey,Elizabeth A.
This study aimed to fingerprint 36 water yam (Dioscorea alata) accessions using microsatellite markers. Ten accessions were collected in local markets from several municipalities in Brazil, eight were obtained from the 'Instituto Agronômico de Campinas' (IAC) germplasm collection and eighteen were collected directly from growers from São Paulo state. A total of nine microsatellite loci were used in the analysis. Loci revealed high polymorphism verified by elevated PIC values (0.57-0.77), and by high gene diversity and Shannon-Wiener indices (0.69 and 1.29 on average, respectively). The accessions were classified into two groups based on clustering analysis. One group contained mostly accessions from the IAC collection, including a commercial cultivar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dioscorea alata; Commercial varieties; Genetic diversity; Germplasm collection; SSR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362012000400015

Characterization of pepper accessions using molecular markers linked to pungency and SSR
Provedor de dados: 75
Autores: Jesus,Rafaela de; Santos,Gabriel do N; Piccin,Andressa S; Balsalobre,Thiago WA; Sala,Fernando C; Carneiro,Monalisa S.
ABSTRACT Peppers of the genus Capsicum are of great socioeconomic importance, being pungency trait their main attraction. Pungency characterization, genetic distance estimates and population structure analysis of the accessions belonging to germplasm banks are important for parent selection which allows to obtain superior progenies. Therefore, the aims of this study were: i) evaluate 81 accessions of the Capsicum spp. Germplasm Bank of Universidade Federal de São Carlos (BGC-UFSCar) with molecular markers linked to pungency; ii) estimate the genetic diversity among accessions of the BGC-UFSCar using microsatellite markers (SSR); and iii) evaluate the efficiency of these markers in the distinction among species of Capsicum spp. We noticed that pun11 and SNP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capsicum spp.; SSR; Germplasm; Dissimilarity; Polimorphism.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362019000200152

Estrutura genética de populações silvestres de Oryza glumaepatula em três biomas brasileiros utilizando marcadores microssatélites.
Provedor de dados: 14
Autores: BRONDANI, R. P. V.; RANGEL, P. H. N.; ZUCCHI, M. I.; MAGALHÃES, M. R.; BORBA, T. C. O.; VENCOVSKY, R.; BRONDANI, C.
Oryza glumaepatula, uma das 21 espécies silvestres do gênero Oryza, no qual está incluído o arroz cultivado (O. sativa), constitui uma valiosa fonte doadora de alelos favoráveis para características de interesse agronômico desta espécie. Diversas áreas de ocorrência natural de O. glumaepatula estão sob ameaça de devastação e, desta forma, a implementação de estratégias de conservação destes recursos genéticos a partir de estudos de genética de populações é de extrema relevância. Marcadores moleculares microssatélites, ou SSR, são uma ferramenta útil para investigar questões relacionadas ao sistema de cruzamento, fluxo gênico e estrutura genética das populações naturais de O. glumaepatula. Este trabalho objetivou caracterizar a variabilidade genética e...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Oryza glumaepatula; Silvestre; SSR; Variabilidade genética.; Arroz; Conservação; Marcador Molecular.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/212641

Mapeamento e validação de marcadores microssatélites associados à restauração da fertilidade em sorgo.
Provedor de dados: 14
Autores: PINTO, M. de O.; SANTOS, C. V. dos; MENEZES, C. B. de; PARRELLA, R. A. da C.; SCHAFFERT, R. E.; MAGALHAES, J. V.
Os locos Rf1, Rf2 e Rf5 são responsáveis pela restauração da fertilidade em genótipos de sorgo em citoplasma A1. O objetivo desse trabalho foi mapear e validar marcadores moleculares associados com a restauração da fertilidade em genótipos-elite de sorgo do programa de melhoramento de sorgo sacarino da Embrapa Milho e Sorgo. Foram avaliados 37 locos microssatélites (Simple Sequence Repeats, SSR) na região cromossômica dos locos Rf. Para o mapeamento foram utilizados 166 progênies F2 (CMSXS222B x BR505R) e para a validação foram utilizadas 90 famílias F2:4 (CMSXS157B x BR505R). Apenas os marcadores localizados em uma janela de 47,4 cM, a qual inclui o loco Rf1, apresentaram associação com a restauração da fertilidade na população F2 (CMSXS222B x BR505R),...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Locos Rf; SSR; Mapeamento genético; Seleção assistida; Sorghum bicolor; Marcador molecular; Melhoramento.
Ano: 2014
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1010346

Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
Provedor de dados: 83
Autores: Souza-Shibatta,Lenice; Ferreira,Dhiego Gomes; Oliveira,Claudio; Almeida,Fernanda Simões de; Shibatta,Oscar Akio; Sofia,Silvia Helena.
Thirteen microsatellite loci were isolated and characterized in Microglanis cottoides. Of these, two were monomorphic and 11 were polymorphic. These polymorphic loci tested on 24 individuals from a wild population produced a total of 108 different alleles, with levels of variability high, ranging from 2 to 20, with an average of 8.3 alleles per locus. The observed and expected heterozygosity ranged from 0.125 to 0.958 and from 0.119 to 0.931, respectively. A high combined probability of paternity exclusion value and a low probability combined genetic identity value obtained show that the set of loci described herein displays good suitability for paternity studies and differentiation of M. cottoides. Additionally, all thirteen microsatellite primers...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brazilian Eastern basin; Catfish; Enriched genomic library; Genetic marker; SSR.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300581

Development and characterization of fifteen polymorphic microsatellite loci in Bryconamericus aff. iheringii (Teleostei: Characidae) and cross-amplification in related Characidae species
Provedor de dados: 83
Autores: Apolinário-Silva,Caroline; Ferreira,Dhiego G.; Cavenagh,Analiza F.; Aprígio,Nícollas G. O.; Galindo,Bruno A.; Carlsson,Jens; Sofia,Silvia H.
ABSTRACT Data on 15 novel microsatellite loci from the Neotropical fish Bryconamericus aff. iheringii are presented here. Analyses of 32 individuals from four different streams revealed 192 different alleles, ranging from four to 32 alleles per locus (mean of 12.8 per locus). Observed and expected heterozygosities ranged from 0.094 to 0.813 and 0.205 to 0.952, respectively. These loci showed high polymorphic information content and will be a resource for genetic studies of B. aff. iheringii. Furthermore, several loci also amplified other small Neotropical Characidae (Piabarchus stramineus and Piabina argentea) and should be useful for these species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Lambarizinho; Molecular markers; Neotropical fish; SSR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000100218

Electric eels galore: microsatellite markers for population studies
Provedor de dados: 83
Autores: Souza-Shibatta,Lenice; Ferreira,Dhiego G.; Santos,Kátia F.; Galindo,Bruno A.; Shibatta,Oscar A.; Sofia,Silvia H.; Giacomin,Renata M.; Bastos,Douglas A.; Mendes-Júnior,Raimundo N. G.; Santana,Carlos David de.
Abstract Fourteen novel microsatellite loci are described and characterized in two species of electric eels, Electrophorus variiand E. voltaifrom floodplains and rivers of the Amazon rainforest. These loci are polymorphic, highly informative, and have the capacity to detect reliable levels of genetic diversity. Likewise, the high combined probability of paternity exclusion value and low combined probability of genetic identity value obtained demonstrate that the new set of loci displays suitability for paternity studies on electric eels. In addition, the cross-amplification of electric eel species implies that it may also be useful in the study of the closely related E. electricus, and to other Neotropical electric fishes (Gymnotiformes) species as tested...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazon Rainforest; Electrophorus; Genetic diversity; Gymnotiformes; SSR.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000400202

第40回アメリカ生殖学会 (40th Annual Meeting of the Society for the Study of Reproduction) への参加及び発表
Provedor de dados: 19
Autores: 笹原, 希笑実.
Palavras-chave: SSR; サンアントニオ; 黄体.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1824

Identificação de marcadores microssatélites relacionados ao escurecimento de grãos em feijão
Provedor de dados: 86
Autores: Couto,Karla Rodrigues; Santos,João Bosco dos; Ramalho,Magno Antônio Patto; Silva,Graciele Simoneti da.
O objetivo deste trabalho foi identificar marcadores microssatélites ligados ao loco de características quantitativas (QTL) responsável pelo escurecimento tardio do tegumento de feijões do tipo carioca, a fim de reduzir o tempo de avaliação necessário para seleção quanto a essa característica. Foram utilizados dados de avaliação fenotípica de 185 progênies F2:3 derivadas do cruzamento VC-3 x 'BRSMG Majestoso', para o estudo do controle genético do escurecimento dos grãos. Com esses dados, foram confeccionados dois "bulks" segregantes de DNA, empregados para a avaliação de 444 pares de primers SSR. Os aplicativos computacionais GQMOL e Sisvar foram utilizados para avaliar as segregações, confeccionar um grupo de ligação e realizar análises de marca simples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Locos de características quantitativas; Marcador molecular; Seleção assistida; SSR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010001100006

Validation of microsatellite markers for assisted selection of soybean resistance to cyst nematode races 3 and 14
Provedor de dados: 86
Autores: Silva,Marcia Flores da; Schuster,Ivan; Silva,João Flávio Veloso da; Ferreira,Adésio; Barros,Everaldo Gonçalves de; Moreira,Maurilio Alves.
The objective of this work was to validate microsatellite markers associated with resistance to soybean cyst nematode (Heterodera glycines Ichinohe) races 3 and 14, in soybean (Glycine max L.) genotypes, for use in marker-assisted selection (MAS) programs. Microsatellites of soybean linkage groups A2, D2 and G were tested in two populations, and their selection efficiencies were determined. The populations were 65 F2:3 families from Msoy8001 (resistant) x Conquista (susceptible) cross, and 66 F2:3 families of S5995 (resistant) x Renascença (susceptible) cross, evaluated for resistance to races 3 and 14, respectively. Families with female index up to 30% were considered moderately resistant. Markers of A2 and G linkage groups were associated with resistance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Heterodera glycines; Soybean breeding; SCN; SSR; MAS.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000800011

Outcrossing rate between 'Haden' and 'Tommy Atkins' mangoes estimated using microsatellite and AFLP markers
Provedor de dados: 86
Autores: Santos,Carlos Antonio Fernandes; Lima Neto,Francisco Pinheiro.
The objective of this work was to estimate outcrossing rates between Haden and Tommy Atkins mango cultivars, using AFLP and microsatellite markers. Progenies of an isolated 'Haden' plant, identified in a 'Tommy Atkins' commercial orchard, in Petrolina, PE, Brazil, were analyzed. Total DNA was isolated from the progeny leaves and used for AFLP and microsatellite reactions. Multilocus outcrossing rates (t m) were estimated by direct count of AFLP or microsatellite markers and by the mLTR software. Outcrossing rates ranged from 0.85 to 0.87 with the analysis based on seven AFLP markers, and from 0.83 to 0.91 based on three microsatellite primers. No unexpected band patterns were observed for 'Haden' and 'Tommy Atkins'. The estimates obtained with the mLTR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mangifera indica; MLTR; Multilocus estimation; SSR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000800016

Correlação da heterose de híbridos de milho com divergência genética entre linhagens
Provedor de dados: 86
Autores: Guimarães,Paula de Souza; Paterniani,Maria Elisa Ayres Guidetti Zagatto; Lüders,Reginaldo Roberto; Souza,Anete Pereira de; Laborda,Prianda Rios; Oliveira,Karine Miranda.
O objetivo deste trabalho foi avaliar a produtividade de híbridos simples de milho obtidos de cruzamentos dialélicos entre linhagens divergentes, estimar a capacidade de combinação das linhagens e confirmar se a divergência genética entre as linhagens, obtida por marcadores moleculares, é correlacionada com a heterose dos híbridos simples no campo. Trinta e seis híbridos resultantes do dialelo parcial e as 12 linhagens parentais foram avaliadas em Campinas em blocos ao acaso, com três repetições e duas testemunhas. A capacidade de combinação das linhagens foi estimada de acordo com o modelo 4 de Griffing. Estimaram-se a correlação matricial, mediante a estatística de Mantel, entre heterose, produtividade e capacidade específica de combinação com...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays; Marcadores moleculares; Dialelo parcial; Capacidade de combinação; AFLP; SSR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000600007

Identificação de QTL associados à simbiose entre Bradyrhizobium japonicum, B. elkanii e soja
Provedor de dados: 86
Autores: Santos,Maria Aparecida dos; Nicolás,Marisa Fabiana; Hungria,Mariangela.
O objetivo deste trabalho foi identificar QTL (locos de caráter quantitativo), utilizando marcadores do tipo microssatélites (SSR), relacionados à fixação biológica de nitrogênio (FBN), em uma população F2:7 de cultivares de soja (Glycine max) com diferentes capacidades de FBN, Bossier (alta) e Embrapa 20 (média). Foram mapeados 16 marcadores, distribuídos em seis grupos de ligação, cobrindo uma região de 5% do genoma (151,6 cM). A análise de regressão identificou 12 associações significativas em quatro grupos de ligação (B1, C2, D1b e H): três para a massa da parte aérea seca, quatro para número de nódulos, duas para a massa de nódulos e três para a massa média de nódulos. Todos os QTL detectados foram de efeitos menores. Contudo, sete marcadores foram...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fixação biológica do nitrogênio; Marcadores moleculares; Mapa genético; Microssatélites; Nodulação; SSR.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000100010

Diversidade genética da soja entre períodos e entre programas de melhoramento no Brasil
Provedor de dados: 86
Autores: Priolli,Regina Helena Geribello; Mendes-Junior,Celso Teixeira; Sousa,Sandra Mara Bispo; Sousa,Neylson Eustáquio Arantes; Contel,Eucleia Primo Betioli.
O objetivo deste trabalho foi detectar os efeitos do melhoramento sobre a diversidade do germoplasma da soja cultivada nas três ultimas décadas, por meio da comparação de seis programas de melhoramento e períodos de lançamento de cultivares, utilizando locos microssatélites. Em relação aos programas de melhoramento, todos os locos apresentaram diferenças significativas em suas distribuições alélicas. Alguns locos eram compostos de alelos exclusivos em alguns programas de melhoramento, enquanto outros foram compostos sempre dos mesmos alelos em maior freqüência para todos os programas. A AMOVA indicou maior porção da variância devido a cultivares dentro de programas e somente 5,3% (p&lt;0,05) devido à diferença entre programas. Quando comparados os...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; SSR; Germoplasma; Erosão genética.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004001000004

Variabilidade genética de Phakopsora pachyrhizi avaliada por meio de marcadores microssatélites
Provedor de dados: 86
Autores: Tschurtschenthaler,Nely Norder; Vieira,Elisa Serra Negra; Nora,Tatiane Dalla; Schuster,Ivan.
O objetivo deste trabalho foi avaliar a variabilidade genética da ferrugem-asiática-da-soja no Brasil, com uso de marcadores microssatélites. Populações de esporos de Phakopsora pachyrhizi coletadas nas regiões Sul, Sudeste e Centro Oeste do país foram submetidas à análise de variabilidade genética, avaliada por meio de marcadores microssatélites específicos para o fungo. Foram coletadas, também, populações de fungo em diversas variedades de soja em uma mesma localidade, incluindo populações com lesão "reddish-brown" (RB). Entre essas populações, não houve variabilidade. Tecidos com lesões RB não apresentaram esporos do fungo e não amplificaram com os marcadores específicos para P. pachyrhizi. A variabilidade genética entre as populações coletadas nas três...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Diversidade genética; Ferrugem-asiática-da-soja; Resistência genética; Resistência vertical; SSR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000200005

New microsatellite markers developed from an enriched microsatellite common bean library
Provedor de dados: 86
Autores: Cardoso,Juliana Morini Kupper; Oblessuc,Paula Rodrigues; Campos,Tatiana de; Sforça,Danilo Augusto; Carbonell,Sérgio Augusto Moraes; Chioratto,Alisson Fernando; Formighieri,Eduardo Fernandes; Souza,Anete Pereira de; Benchimol,Luciana Lasry.
The objective of this work was to develop new microsatellite markers in common bean. Ninety nine new microsatelitte loci were developed from a microsatellite enriched library for (CT)8 and (GT)8 motifs, from CAL-143 line. The majority of microsatellite sequences (51%) was related to cellular metabolism. The remaining sequences were associated to transcription functions. Only 17.2% of the sequences presented some level of similarity with other plant species genes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Molecular markers; Plant breeding; QTL; SSR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000700019

Microsatellites for genetic studies and breeding programs in common bean
Provedor de dados: 86
Autores: Campos,Tatiana de; Benchimol,Luciana Lasry; Carbonell,Sérgio Augusto Moraes; Chioratto,Alisson Fernando; Formighieri,Eduardo Fernandes; Souza,Anete Pereira de.
Twenty microsatelitte loci were identified and characterized in common bean. Microsatellites were tested in 14 genotypes. The allele number ranged from 1 to 3, and the polymorphism information content (PIC) was between 0.14 and 0.65. These polymorphic markers are available to be used for breeding programs.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; SSR; Molecular markers.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000400018

Caracterização molecular de cultivares de cebola com marcadores microssatélites
Provedor de dados: 86
Autores: Santos,Carlos Antonio Fernandes; Oliveira,Valter Rodrigues; Rodrigues,Marciene Amorim; Ribeiro,Hugo Leonardo Coelho.
O objetivo deste trabalho foi estabelecer padrões alélicos e estimar as distâncias genéticas baseadas em marcador SSR de 44 cultivares de cebola adaptadas às condições de cultivo tropicais e subtropicais do Brasil. Para visualização da similaridade genética, utilizou-se o dendrograma UPGMA gerado da matriz de distâncias do coeficiente de Jaccard, com base em 40 alelos de 13 locos SSR. O DNA total foi extraído pelo método CTAB 2x, e os produtos de PCR foram analisados em géis de poliacrilamida desnaturante 6% e corados com nitrato de prata. O número de pares de bases foi estimado pelo método da mobilidade inversa, com base em regressão de produtos de tamanho conhecido. A média da heterozigosidade dos locos SSR foi 0,58. Os 40 alelos dos 13 locos SSR foram...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allium cepa; Dendrograma; Similaridade; SSR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000100007

Identificação e validação de marcadores microssatélites ligados ao gene Rpp5 de resistência à ferrugem-asiática-da-soja
Provedor de dados: 86
Autores: Morceli,Thaiza Galhardo Silva; Trevisoli,Sandra Helena Unêda; Morceli Junior,Antonio Ayrton; Kiihl,Romeu Afonso de Souza; Calvo,Eberson Sanches; Di Mauro,Antonio Orlando; Garcia,Alexandre.
O objetivo deste trabalho foi identificar novos marcadores microssatélites, ligados ao gene Rpp5 de resistência à ferrugem-da-soja, e validar os marcadores previamente mapeados, para que possam ser utilizados em programas de seleção assistida por marcadores moleculares (SAM). Para tanto, uma população F2 com 100 indivíduos, derivada do cruzamento entre a PI 200526 e a cultivar Coodetec 208, suscetível à ferrugem, foi artificialmente infectada e avaliada quanto à sua reação de resistência à ferrugem. Marcadores microssatélites foram testados nos genitores e em dois "bulks" contrastantes, para a identificação de marcadores ligados. Dois novos marcadores, potencialmente associados à resistência, foram testados em plantas individuais, e se constatou que eles...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Phakopsora pachyrhizi; Seleção assistida por marcadores; SSR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001100011

Otimização da utilização de marcadores moleculares microssatélites e sua aplicação em estudos com plantas daninhas
Provedor de dados: 87
Autores: Goulart,I.C.G.R; Merotto Junior,A; Nunes,A.L; Bered,F.
Apesar de existirem marcadores moleculares mais específicos, os marcadores microssatélites apresentam grande potencialidade de utilização na área de plantas daninhas devido à sua crescente disponibilização em outras espécies e à qualidade das informações proporcionadas. O uso convencional dos marcadores moleculares microssatélites demanda grande quantidade de trabalho e recursos financeiros. O objetivo deste trabalho foi descrever a técnica da cauda fluorescente como forma de otimização da utilização de marcadores moleculares microssatélites, utilizando como exemplo um estudo de identificação de híbridos entre arroz-vermelho e cultivado. Foram utilizadas como modelo plantas de arroz cultivado, arroz-vermelho e o híbrido originado do cruzamento artificial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arroz-vermelho; Cauda M13; Hibridização; Primer marcado; Simple sequence repeats; SSR.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582011000500025

Genetic Dissimilarity in Conyza sumatrensis Revealed by Simple Sequence Repeat (SSR) Markers
Provedor de dados: 87
Autores: SCHNEIDER,T.; RIZZARDI,M.A.; BRAMMER,S.P.; SCHEFFER-BASSO,S.M.; NUNES,A.L.
ABSTRACT: In view of the rapid evolution of Conyza sumatrensis populations resistant to glyphosate, it is necessary to understand the genetic diversity aimed to improve strategies for managing this weed. We investigated the genetic dissimilarity among 15 biotypes of C. sumatrensis from different geographic regions using microsatellite loci. The biotypes, were cultivated in a greenhouse to obtain vegetal material for DNA extraction. Nineteen microsatellite markers (SSR), were developed for C. sumatrensis biotypes. The genetic dissimilarity was estimated by the Jaccard coefficient (JC) and the biotypes grouped by the UPGMA method. The results demonstrated a high dissimilarity (JC = 7.14 to 82.62) of the analyzed material, with the biotypes forming five...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polymorphism; SSR; Tall fleabane; Genetic variability.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582020000100218

Caracterização de genótipos de mirtilo utilizando marcadores moleculares
Provedor de dados: 94
Autores: Silva,Sergio Delmar dos Anjos e; Antunes,Luis Eduardo Correa; Anthonisen,Denilson Gouvea; Lemões,Juliana Silva; Gonçalves,Emerson Dias.
O cultivo do mirtilo está em expansão no Brasil, em especial em regiões de clima temperado, onde há grande demanda em relação a cultivares adaptadas às condições edafoclimáticas regionais. O objetivo deste trabalho foi caracterizar genótipos de mirtilo do programa de melhoramento da Embrapa Clima Temperado, utilizando marcadores moleculares do tipo RAPD e SSR. Foram caracterizados 40 genótipos de mirtilo por RAPD e oito cultivares por microssatélites. Os nove primers utilizados na técnica de RAPD geraram 89 marcadores. A similaridade genética entre os genótipos variou de 64 a 89%. Utilizando a similaridade média (66%), foram obtidos quatro grupos. Foram gerados 11 marcadores a partir de três pares de primers de microssatélites. A similaridade genética...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RAPD; SSR; Vaccinium.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452008000100033

Identificação de microssatélites para o mamoeiro por meio da exploração do banco de dados de DNA
Provedor de dados: 94
Autores: Oliveira,Eder Jorge de; Dantas,Jorge Luiz Loyola; Castellen,Milene da Silva; Machado,Marlos Dourado.
Os marcadores microssatélites são ferramentas úteis em diversas análises genéticas em plantas. No caso do mamoeiro (Carica papaya L.), poucos locos de microssatélites foram descritos até o momento. Assim, o objetivo deste trabalho foi explorar a base de dados do GenBank / NCBI (National Center of Biotechnoloy Information) à procura de microssatélites de mamoeiro, visando a seu futuro uso em estudos genéticos e moleculares aplicados ao melhoramento genético. As seqüências foram obtidas no GenBank / NCBI, no formato FASTA, e analisadas para a presença de microssatélites com um mínimo de 20; 7 e 5 repetições dos motivos de mono-, di- e trinucleotídeos, respectivamente, e acima de 4 repetições para tetra- e pentanucleotídeos. Seqüências com mais de 90% de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marcador molecular; Carica papaya L.; SSR; GenBank.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452008000300049

Caracterização molecular e diversidade genética de diferentes variedades de abacate por marcadores microssatélites
Provedor de dados: 94
Autores: Martins,Antonio Baldo Geraldo; Rodrigues,Maria Gabriela Fontanetti; Paula,Deise Reis de; Mendes,Hélio Sandoval Junqueira; Arantes,Flávio Cese; Silva,Cristina Lacerda Soares Petrarolha.
Pertencente à família Lauraceae, o abacateiro compreende três raças hortícolas: antilhana, guatemalense e mexicana. Os marcadores moleculares são uma ferramenta rápida e eficaz para estudos genômicos, uma vez que detectam o polimorfismo diretamente ao nível do DNA e não sofrem qualquer tipo de influência ambiental. Com base nesse polimorfismo, é possível fazer inferências sobre as relações entre o genótipo e o fenótipo dos indivíduos, o que, em última análise, permite aumentar a eficiência dos programas de melhoramento. Diante o exposto, o objetivo foi investigar a diversidade genética entre sete variedades de abacate a partir de 5 lócus de marcadores moleculares microssatélites (SSR). Nas amostras de abacateiros avaliadas, encontrou-se um total de 18...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Persea americana; SSR; Fingerprinting; Banco de germoplasma.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452011000400016

CARACTERÍSTICAS MORFOLÓGICAS E VARIABILIDADE GENÉTICA DE ARATICUM UTILIZANDO MARCADORES RAPD E MICROSSATÉLITES
Provedor de dados: 94
Autores: PIRES,MARCELA VERSIANI VENÂNCIO; FALEIRO,FÁBIO GELAPE; SILVA,JOSÉ CARLOS SOUSA; MELO,JOSÉ TEODORO DE; PEIXOTO,JOSÉ RICARDO.
RESUMONeste trabalho, objetivou-se avaliar a variabilidade genética da coleção de trabalho de acessos de araticum da Embrapa Cerrados e outros materiais próximos ao Distrito Federal, utilizando marcadores moleculares RAPD, microssatélites e análise de características morfológicas. Folhas de 18 acessos de araticum foram coletadas e utilizadas para a extração das amostras de DNA genômico, as quais foram amplificadas para obtenção de marcadores moleculares RAPD e microssatélites. Na análise morfológica, foram avaliadas 23 características dos acessos de araticum. As dissimilaridades genéticas entre os 18 genótipos de araticum evidenciaram a variabilidade genética dos acessos e as análises de agrupamento levaram à formação de três grupos de similaridade....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marcadores moleculares; SSR; Caracterização; Recursos genéticos.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452015000100149

Diversity and genetic relatedness among genotypes of Vitis spp. using microsatellite molecular markers
Provedor de dados: 94
Autores: Leão,Patrícia Coelho de Souza; Cruz,Cosme Damião; Motoike,Sérgio Yoshimitsu.
The purpose of this research was to study the genetic diversity and genetic relatedness of 60 genotypes of grapevines derived from the Germplasm Bank of Embrapa Semiárido, Juazeiro, BA, Brazil. Seven previously characterized microsatellite markers were used: VVS2, VVMD5, VVMD7, VVMD27, VVMD3, ssrVrZAG79 and ssrVrZAG62. The expected heterozygosity (He) and polymorphic information content (PIC) were calculated, and the cluster analysis were processed to generate a dendrogram using the algorithm UPGMA. The He ranged from 81.8% to 88.1%, with a mean of 84.8%. The loci VrZAG79 and VVMD7 were the most informative, with a PIC of 87 and 86%, respectively, while VrZAG62 was the least informative, with a PIC value of 80%. Cluster analysis by UPGMA method allowed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Grape; Multivariate analysis; Genealogy; SSR.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452013000300017

Marcadores moleculares microssatélites na avaliação de sementes de soja com variação na coloração do hilo
Provedor de dados: 95
Autores: Rabel,Marcelo; Vieira,Elisa Serra Negra; Lana,Ubiraci Gomes de Paula; Paiva,Edilson; Sehnem,Margarete Aparecida Sella; Schuster,Ivan.
Marcadores microssatélite têm sido utilizados na determinação da pureza genética em programas de melhoramento e de produção de sementes, visando garantir a qualidade genética das sementes. Objetivou-se neste trabalho demonstrar, com auxílio de marcadores moleculares microssatélites, que variações na coloração do hilo em sementes de soja, muitas vezes não constituem variação genética. Foram avaliadas sementes de soja da cultivar CD 222 que apresenta sementes com hilo preto, e de duas linhagens (CD 02RV-8444 e CD 01RV-7618), as quais apresentam sementes com hilo marrom. As sementes com variações na coloração e tonalidade do hilo encontradas em cada genótipo foram separadas visualmente em grupos. O DNA de cada semente foi extraído e analisado com marcadores...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Cor do hilo; Pureza genética; SSR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222010000200002

Genetic monitoring of a Santa Ines herd using microsatellite markers near or linked to the sheep MHC
Provedor de dados: 96
Autores: Petroli,César Daniel; Paiva,Samuel Rezende; Corrêa,Marilma Pachêco Chediak; McManus,Concepta.
This study aimed to analyze genetic diversity in a conservation nucleus of Santa Inês sheep using thirteen microsatellite loci on chromosome 20 (where the Sheep Major Histocompatibility Complex - Ovar-MHC - is found). Seventy three animals from one herd born from 2004 to 2006 were evaluated as a principal nucleus. Seventy one animals from two other herds were used as control comparison. There was a reduction in heterozygosity over the years in relation to the whole population. This may be due to the repeated use of the same sires. The estimates of molecular coancestrality also indicated an increase in genetic similarity between individuals with the herd over the years. A high number of alleles occurred exclusively in the principal nucleus herd, but with a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal genetic resources; Genetic diversity; Ovar-MHC; Ovis aries; Molecular coancestrality; SSR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009000400012

Microsatellite markers for identification of Prunus spp. rootstocks
Provedor de dados: 63
Autores: Bianchi,Valmor João; Sansavini,Silviero; Fachinello,José Carlos.
Cultivar characterization for fruit trees certification requires fast, efficient and reliable techniques. Microsatellite markers (SSR) were used in the molecular characterization of 29 Prunus spp. rootstocks. The DNA from the rootstocks was analyzed using five pre-selected SSR primers (UDP96-005, UDP96-008, UDP96-013, UDP96-18 and UDP98-414) and revealed 81 alleles, which allowed each genotype to be identified. The UDP96-005 marker generated the most information, i.e., 23 well-distributed, polymorphic alleles among all genotypes. The 21 polymorphisms produced by UDP96-013 occurred mainly as a result of high degree of variability among genotypes of the Prunophora subgenus. In the dendrogram, the five markers allowed the 29 rootstocks to be grouped into...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peach tree; SSR; Fingerprinting.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000300011

Morphological and molecular characterization of Puccinia kuehnii, the causal agent of sugarcane orange rust, in Cub
Provedor de dados: 63
Autores: Perera,María Francisca; Bertani,Romina Priscila; Arias,Marta Eugenia; Hechavarría,María de la Luz La O; Navarro,María de los Ángeles Zardón; Debes,Mario Alberto; Luque,Ana Catalina; Cuenya,María Inés; Rojas,Ricardo Acevedo; Castagnaro,Atilio Pedro.
ABSTRACT Sugarcane orange rust caused by Puccinia kuehnii has recently become an important disease in sugarcane crops and its spread is causing great concern to growers. In this study, we analyzed spores from symptomatic orange rust sugarcane leaves collected in multiple locations in Cuba in a 4-year-period in order to characterize morphological traits of P. kuehnii, establish an adequate molecular technique to characterize it, and determine its infection court in sugarcane. Orange rust caused by P. kuehnii was confirmed by polymerase chain reaction (PCR) and morphological characterization. AFLP markers detected high diversity in P. kuenhnii samples. Sequencing of rDNA regions, as expected, did not reveal differences and SSR markers designed for P....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; RDNA; SSR; Infection courts.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000201200

Genetic structure analysis of Mauritia flexuosa natural population from the Lençóis Maranhenses region using microsatellite markers
Provedor de dados: 63
Autores: Castro,Domingos Augusto Mendonça; Costa,Tatiana Santos; Cardoso,Ariane Santos; Ramos,Helaine Christine Cancela; López,Jorge Alberto; Diniz,Leandro Eugenio Cardamone.
ABSTRACT Buriti (Mauritia flexuosa L.f), a palm tree native to South America and widely distributed in Brazil, displays significant ecological, economic and biotechnological importance. However, the disorderly extractivism and environmental degradation of its endemic areas are leading to reductions in, and/or extinction of the buriti palm tree, causing ecological imbalance with significant economic losses for rural communities and genetic diversity. Consequently, populational genetic diversity studies have become relevant as a strategy for conserving the species. Therefore, this study evaluated the genetic structure of 10 populations from the Lençóis Maranhenses region, in the state of Maranhão, Brazil, using microsatellite markers. Results indicated that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Buriti; SSR; Preservation; Diversity; Variability.
Ano: 2022
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162022000101101

CTAB methods for DNA extraction of sweetpotato for microsatellite analysis
Provedor de dados: 63
Autores: Borges,Aline; Rosa,Mariana Silva; Recchia,Gustavo Henrique; Queiroz-Silva,Jurema Rosa de; Bressan,Eduardo de Andrade; Veasey,Elizabeth Ann.
Microsatellite markers have proved to be useful in genetic diversity assessments of sweetpotato (Ipomoea batatas) but practical DNA extraction methods to ensure good quality and quantity DNA for these studies are yet to be established. This study compares the efficiency of three modified methodologies for DNA extraction of six sweetpotato landraces using the CTAB extraction buffer in regard to quantity and purity of DNA quantification and microsatellite band patterns. All methodologies yielded satisfactory results, but the method based in leaf tissue macerated in liquid nitrogen was deemed more adequate because of its simplicity and lower cost. However, the method based in dry leaf tissue was considered more advantageous, first because elicits...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ipomoea batatas; SSR; DNA isolation; Landraces; Protocol.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000400015

Structure of genetic diversity of Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae) populations in Brazilian crops and locations
Provedor de dados: 63
Autores: Fontes,Fernanda von Hertwig Mascarenhas; Colombo,Carlos Augusto; Lourenção,André Luiz.
Bemisia tabaci (Genn.) represents a complex of cryptic species that causes losses of many valuable crops. Even though there are differences in their ability to colonize hosts, transmit phytovirus and develop resistance to insecticides, the genetic differentiation of the insect populations is important for the adoption of control measures. Therefore, the genetic diversity of B. tabaci populations in economically important crops in Brazilian locations was characterized through a microsatellite analysis. Eight microsatellite markers were used for the analysis of eight populations, three in Solanum tuberosum (States of São Paulo, Minas Gerais and Bahia), two in Glycine max (States of São Paulo and Mato Grosso), one in Phaseolus vulgaris and Brassica oleracea...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; Biotype B; Population genetics; Silverleaf whitefly.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000100007

การใช้เทคนิค Simple sequence repeat (SSR) ตรวจ polymorphism ของ DNA ในข้าวโพด
Provedor de dados: 106
Autores: Chaba Jampatong; Chatpong Balla; Gunyavee Saipun.
12 ill., 3 tables
Palavras-chave: Corn; Maize; DNA polymorphism; SSR; ข้าวโพด; โพลีมอร์ฟิสซึม; ดีเอนเอ; เทคนิคเอสเอสอาร์; การสกัด.
Ano: 2004
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/4212

Identificación de QTLs para carotenos en el genoma de yuca, (Manihot esculenta Crantz), y validación en poblaciones S1
Provedor de dados: 109
Autores: Morillo C,Ana Cruz; Morillo C,Yacenia; Ceballos L,Hernán.
El contenido de b-caroteno en yuca (Manihot esculenta Crantz) es importante para suplir las deficiencias de vitamina A en regiones donde esta raíz es la principal fuente de alimento. Las familias de esta especie utilizadas para el análisis molecular del contenido de carotenos fueron: GM 708, GM 734 y CM 9816. Se utilizó el Análisis de Grupos Segregantes (Bulked Segregant Analysis, BSA por su sigla en inglés) para la evaluación de 800 marcadores microsatélites. Para determinar la asociación con el color del parénquima de la raíz se hizo un análisis de correlación y regresión simple entre los datos fenotípicos y los genotípicos de cada marcador. Se hizo, además, un análisis de QTLs con estas familias y se validó su presencia en descendencias S1. Se encontró...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carotenos; Manihot esculenta Crantz; Mapeo genético; QTLs; SSR.
Ano: 2013
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122013000300002

Mapeo genético y análisis de QTL para carotenos en una población s1 de yuca
Provedor de dados: 109
Autores: Marín Colorado,Jaime Alberto; Ramírez,Hernando; Fregene,Martin.
La población S1 de la variedad tailandesa de yuca MTAi8 (AM320), la cual presenta patrones de segregación definidos para el contenido de carotenos totales (Beta-caroteno), se sometió a un análisis de agrupamiento segregante (BSA = Bulk Segregant Analysis) empleando 700 marcadores moleculares tipo microsatélites o SSR. Se generaron 25 grupos de ligamiento identificando 3 QTL mayores asociados con una región del genoma de yuca con el contenido de carotenos totales. Tres marcadores SSR explicaron el 37.2% (NS109), 32% (rSSRY251) y 27.7% (rSSRY313) de la varianza fenotipica total, situados en el grupo de ligamiento D fuertemente asociados con el contenido de carotenos totales en la familia AM320 S1, se estableció una fuerte correlación positiva entre color de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta Crantz; Caroteno; QTL; SSR.
Ano: 2009
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122009000100003

Transferability of heterologous primers in Brycon falcatus
Provedor de dados: 36
Autores: PENHA,Diego dos Santos; SOUZA,Felipe Pinheiro de; LIMA,Ed Christian Suzuki de; URREA-ROJAS,Angela Maria; PANDOLFI,Victor César Freitas; YAMACHITA,Andrei Lincoln; POVH,Jayme Aparecido; LEITE,Natalia Gonçalves; PEREIRA,Ulisses de Pádua; LOPERA-BARRERO,Nelson Mauricio.
ABSTRACT The genusBryconcomprises fish species of significant socioeconomic and biological importance in Brazil. Despite that, the genetic knowledge about these species is scarce, especially regardingBrycon falcatus. Thus, the objective of this study was to evaluate the transferability of heterologous microsatellite primers inB. falcatus for the first time. Heterologous primers obtained from B. opalinus, B. hilarii, B. insignis, B. orbignyanus, B. amazonicus, Prochilodus argenteus, Prochilodus lineatus, Piaractus mesopotamicus, and Colossoma macropomum were evaluated. The primers that showed the best amplification patterns were applied to a sample of 22 individuals and the genetic parameters were calculated. Nine primers displayed satisfactory...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bryconidae; Cross amplification; Genetic conservation; Genetic variability; SSR.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000300232

Inferring mating system parameters in forage peanut, Arachis pintoi, for Brazilian Amazon conditions
Provedor de dados: 36
Autores: OLIVEIRA,Jônatas Chagas de; RUFINO,Polinar Bandeira; AZÊVEDO,Hellen Sandra Freires da Silva; SOUSA,Adna Cristina Barbosa de; ASSIS,Giselle Mariano Lessa de; SILVA,Lucielio Manoel da; SEBBENN,Alexandre Magno; CAMPOS,Tatiana de.
ABSTRACT The search for alternatives to increase productivity and sustainability of livestock production in the Amazon region without increasing deforestation is challenging. Mixed pastures of grasses with forage peanut (Arachis pintoi) have shown positive economic impacts. However, gaps in the knowledge of the reproductive biology of A. pintoi have limited the development of new cultivars adapted to the environmental variations in the Brazilian Amazon. Pasture consortiums of Brachiaria humidicola with forage peanuts (cv. Mandobi) resulted in a 42% increase in weight gain productivity. New cultivars better adapted to the Amazon climate should bring even greater gains. We evaluated the mating system in twenty A. pintoi accessions, and approximately 40...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reproductive system; Arachis pintoi; SSR; Crossing rate.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000400277

Cross-amplification and characterization of microsatellite markers in species of Manihot Mill. (Euphorbiaceae) endemic to the Brazilian Cerrado
Provedor de dados: 39
Autores: Miranda,Kássia Marques Corrêa; Guimarães,Rejane Araújo; Silva,Marcos José da; Oliveira,Patrícia Rasteiro Ordiale; Ribeiro,Thania Gonçalves; Mendes,Thainara Policarpo; Telles,Mariana Pires de Campos; Soares,Thannya Nascimento.
ABSTRACT The genus Manihot Mill. contains about 120 species of which about 104 occur in Brazil. We tested the cross-amplification of ten microsatellite markers developed for Manihot esculenta in 15 species of Manihot endemic to the Brazilian Cerrado. We also evaluated the genetic diversity of Manihot irwinii, M. orbicularis, and M. purpureocostata. Ten pairs of primers were amplified among 14 species of Manihot. The percentage of polymorphic loci per species varied from 70 to 100 %. Nine markers showed amplification and polymorphism when evaluated on polyacrylamide gel. The markers were combined to form three sets for multiplex genotyping for genetic diversity analysis, and showed 51, 75, and 75 alleles in M. irwinii, M. orbicularis, and M....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Polymorphism; SSR; Transferability wild cassava.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000400772

Discovery and characterization of SSR markers in Eugenia uniflora L. (Myrtaceae) using low coverage genome sequencing
Provedor de dados: 42
Autores: SARZI,DEISE S.; JUSTOLIN,BEATRIZ; SILVA,CLARIANE DA; LEMOS,RAFAEL P.M.; STEFENON,VALDIR MARCOS.
Abstract: Eugenia uniflora L. (Myrtaceae) is a tree species widely distributed in South America suffering the effects of the exploitation of natural populations. In this study, we employed low coverage sequencing of the E. uniflora genome for mining of SSR markers. The de novo assembly generated 2,601 contigs with an average length of 1139 bp and spans 3.15 Mb. A total of 76 dimer, 33 trimer and two compound SSR loci were identified. Twelve selected SSR loci were employed to genotype 30 individuals from two natural populations. A total of 73 alleles were detected (mean A= 6.1) were observed, the mean effective number of alleles was Ae = 3.91, mean Ho was 0.23 and mean HE was 0.70). The mean Wright’s within population fixation index was FIS = 0.66 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Next generation sequencing; Pitanga; Population genetics; SSR; Surinam-cherry.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100625

Transferability and characterization of microsatellite markers in five Bromeliaceae species belonging to the subfamilies Pitcairnioideae and Bromelioideae
Provedor de dados: 49
Autores: Miranda,Fabio Demolinari de; Gontijo,Andreia Barcelos Passos Lima; Santiliano,Fabiano Costa; Favoreto,Fernanda Campanharo; Soares,Taís Cristina Bastos.
Microsatellite markers previously developed for Pitcairnia albiflos Herb. and Pitcairnia geysksii L.B.Sm. were used in cross-amplification tests of five other Bromeliaceae species. Ten (76.9%) out of the 13 evaluated pair of primers had positive results for some of the species tested. The number of polymorphic alleles ranged between two and four in most species. Ho values ranged between zero, in Pitcairnia flammea Lindl. (PaA05), Aechmea ramosa Mart ex Schult &amp;amp; Schult and Billbergia horrida Regel (PaC05), and one in Billbergia euphemiae E. Morren (PaA05, PaA10, PaC05 and PaD07). This study showed that microsatellite markers developed for P. albiflos and P. geysksii effectively amplified the DNA samples of Pitcairnia flammea, Aechmea nudicaulis (L.)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cross-amplification; SSR; Genetic diversity; Bromeliads; Conservation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032012000300032

Capacidade combinatória, divergência genética entre linhagens de milho e correlação com heterose
Provedor de dados: 53
Autores: Paterniani,Maria Elisa Ayres Guidetti Zagatto; Guimarães,Paula de Souza; Lüders,Reginaldo Roberto; Gallo,Paulo Boller; Souza,Anete Pereira de; Laborda,Prianda Rios; Oliveira,Karine Miranda.
Objetivou-se avaliar o desempenho de híbridos simples de milho (Zea mays L.), obtidos de cruzamentos dialélicos entre linhagens divergentes, estimar a capacidade de combinação das linhagens e verificar se a divergência genética entre as linhagens, obtida por marcadores moleculares, é correlacionada com a heterose dos híbridos simples no campo. Trinta e seis híbridos resultantes de um dialelo parcial foram avaliados em Campinas e em Mococa e as 12 linhagens parentais somente em Campinas, a fim de se calcular a heterose dos híbridos. O delineamento experimental empregado foi o de blocos ao acaso, com três repetições e 2 testemunhas comerciais. Avaliaram-se os caracteres: altura da planta (AP), altura da espiga (AE) e massa de grãos (MG). Análises de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays; Dialelo parcial; Produtividade; AFLP; SSR.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052008000300012

Novel Microsatellite Markers for Brazilian Mangrove Oysters ( Crassostrea gasar ) and their Cross-Amplification in Crassostrea rhizophorae
Provedor de dados: 52
Autores: Baldez,Renata do Socorro Corrêa; Melo,Mauro André Damasceno; Sampaio,Iracilda; Tagliaro,Claudia Helena.
ABSTRACT A microsatellite CT/GT enriched genomic library was developed for Crassostrea gasar and twelve new polymorphic loci were isolated and characterized. The markers were successfully amplified from 25 individuals of Crassostrea gasar and 11 cross-amplified individuals of Crassostrea rhizophorae. There was no evidence of linkage between loci in either species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; Genetic markers; Cupped oyster; Population structure.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100426

Morphological, Chemical and Molecular Characterization of ‘Old olive’ (Olea europaea L.) from Montenegro
Provedor de dados: 52
Autores: Adakalic,Mirjana; Lazovic,Biljana.
ABSTRACT Thousands of years of tradition of olive cultivation in Montenegro is witnessed with the ‘Old olive’ estimated to be more than 2000 years old. ‘Old olive’ draws attention to its genetic potential and the relation to the closest grown olive cultivars. Therefore, the aim of this paper was to give the basic description of ‘Old olive’ (through 20 morphological and 37 chemical properties) as well as to determine its DNA profile and to examine the closeness with major olive cultivar grown, cv. ‘Žutica’. The obtained data showed the differences between examined samples. ‘Old olive’ started flowering (27. May) more than 10 days later than cv. ‘Žutica’ (14. May), and began ripening 15 days later (20th October and 5th October, respectively). From 21...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ‘Old olive’ tree; Morphology; Oil; SSR; Cv. ‘Žutica’; Montenegro.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100432

Cross-species amplification of microsatellite loci developed for Passiflora edulis Sims. in related Passiflora Species
Provedor de dados: 52
Autores: Oliveira,Gilmara Alvarenga Fachardo; Pádua,Juliano Gomes; Costa,Juliana Leles; Jesus,Onildo Nunes de; Carvalho,Fabiana Moraes de; Oliveira,Eder Jorge de.
The aim of this study was to evaluate the selected 41 SSR markers developed for yellow passion fruit (Passiflora edulis f. flavicarpa Sims.) for their transferability to 11 different Passiflora species. Twenty-one SSR were successfully amplified in 10 wild species of passion fruit producing 101 bands. All the markers were amplifiable for at least one species. The mean transferability was 68,8%, ranging from 15,4% (primer PE11) to 100 % (PE13, PE18, PE37, PE41 and PE88). Transferability was higher for the species from the Passiflora subgenus than for those from the Decaloba and Dysosmia subgenus. The results indicated a high level of nucleotide sequence conservation of the primer regions in the species evaluated, and consequently, they could potentially be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Transferability; SSR; Passion fruit.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500009

Genetic Characterization of Old Grapevines collected in Oases of the Atacama Desert
Provedor de dados: 117
Autores: Poblete,Ingrid; Pinto,Manuel; de Andrés,María Teresa; Hinrichsen,Patricio.
Old grapevine (Vitis vinifera L.) accessions are a source of genes that could be rescued for use per se or in modern breeding programs. The first step in this rescuing is collecting and characterizing the germplasm from a particular region. This study presents the genetic characterization of 21 grapevine accessions collected from the Atacama Desert in the far North of Chile. Characterization was based on 12 microsatellites (Simple Sequence Repeats, or SSRs) supplemented with Amplified Fragment Length Polymorphic (AFLP) markers. Most of the collected accessions produced red berries and shared the genetic characteristics of the cv. País, an old genotype found throughout America. However, among those red-berried accessions, one showed a severe abortive...
Tipo: Journal article
Palavras-chave: SSR; AFLP; Atacama Desert; Germplasm; ‘Listán Prieto’.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000300020

Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers
Provedor de dados: 118
Autores: Alem,Diego; Narancio,Rafael; Díaz Dellavalle,Paola; Rebuffo,Mónica; Zarza,Rodrigo; Dalla Rizza,Marco.
Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive process of isolating Simple Sequence Repeats (SSR, also called microsatellites), the possibility of using microsatellites already identified in related species is highly attractive. The aim of this work was the identification and validation of transferable microsatellite markers in L. corniculatus, and using those markers to study the genetic variability among four cultivars. Each cultivar of L. corniculatus was represented by 15 genotypes. Ten microsatellite...
Tipo: Journal article
Palavras-chave: Genetic variability; Lotus japonicus; SSR.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000300015

Identification and characterization of an original grapevine cultivar (Vitis vinifera) found in Chile
Provedor de dados: 118
Autores: Gonzalez,Alvaro S; Massera,Franco; Moscoso,Daniel; Hinrichsen,Patricio; Montenegro,Gloria; Laucou,Valerie; Lacombe,Thierry; Boursiquot,Jean-Michel; Pszczólkowski,Philippo.
Currently, many monovarietal vineyards present a minority of mixed cultivars. Some of these cultivars are unknown and could represent an opportunity to discover new and potentially useful genotypes both for research and production purposes. In a 'Carmenère' vineyard planted in 1994 in Palmilla (Colchagua Valley, Chile), a minority presence of other cultivars was found. The present work covers for the first time the identification and characterization of one of these cultivars, which presented a very poor fruit set. Through classic ampelography, it was not possible to associate the studied cultivar with any known cultivar in Chile. However, through a simple sequence repeats (SSR) comparison against the database of the INRA Germplasm Repository "Domaine de...
Tipo: Journal article
Palavras-chave: Ampelography; Chile; Coulure; Molecular markers; Poor fruit set; SSR; Triploid.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000200016

Diversidade genética por marcadores moleculares em Fusarium oxysporum f. sp. cubense no Estado de Santa Catarina
Provedor de dados: 65
Autores: Silva,Cristiane Maria da; Hinz,Robert Harri; Stadnik,Marciel João; Pereira,Adriana; Tcacenco,Fernando Adami.
O mal-do-panamá, causado por Fusarium oxysporum f. sp. cubense (FOC), é um dos principais problemas fitossanitários da bananicultura. O uso de cultivares resistentes é o método preferencialmente recomendado para o seu controle, sendo a avaliação da diversidade genética do patógeno necessária no desenvolvimento de estratégias de manejo da doença a longo prazo. Assim, este trabalho teve como objetivo estudar a variabilidade genética de isolados de FOC por marcadores moleculares RAPD e SSR. Foram avaliados 64 isolados coletados em regiões produtoras do estado de Santa Catarina, sendo que 100% deles foram patogênicos à bananeira da cv. 'Enxerto'. As análises de conglomerados com esses marcadores revelaram variabilidade entre os isolados amostrados. As técnicas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Banana; Doença; Fungo; RAPD; SSR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001200007

A new, harmless, high-throughput endosperm-based DNA extraction method for wheat
Provedor de dados: 65
Autores: Ma,Zhihui; Wang,Yuquan; Wei,Wenhui; Ru,Zhengang.
ABSTRACT:  In this study, a non-destructive, high-throughput, endosperm-based DNA extraction method was developed. To verify the non-destructive nature of this method, a germination test was performed on 288 seeds after sampling their endosperm, which gave a seedling emergence rate that was higher (97.6%) than that of the control group (92%). To confirm the feasibility of the new method, DNA was extracted from plants of a BC1F2 population by two different methods, namely, from endosperm using our rapid, high-throughput method (ER-DNA) and from young leaves emerging from the same sampled seed using the CTAB method (LC-DNA). The ER-DNA was undetectable by agarose gel electrophoresis, but was found to be an adequate replacement for LC-DNA for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wheat; Triticum aestivum; SSR; Segregation population.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000900200

Transferability of heterologous microsatellite primers in Brycon gouldingi
Provedor de dados: 65
Autores: Souza,Felipe Pinheiro de; Lima,Ed Christian Suzuki de; Leite,Natalia Gonçalves; Urrea-Rojas,Angela Maria; Yamachita,Andrei Lincoln; Pandolfi,Victor César Freitas; Lopera-Barrero,Nelson Mauricio.
ABSTRACT:  Brycon gouldingi is a species of neotropical fish of socioeconomic and environmental importance in the Tocantins-Araguaia Basin. Genetic studies on this species are still limited, making it difficult to evaluate the population structure and genetic diversity in natural and captive stocks. Here, we aimed to evaluate the transferability of heterologous microsatellite primers in B. gouldingi. A total of 30 primers for eight species were evaluated: Brycon hilarii, Brycon opalinus, Brycon cephalus, Brycon orbignyanus, Prochilodus lineatus, Prochilodus argenteus, Piaractus mesopotamicus, and Colossoma macropomum. The primers that showed the best amplification patterns were applied to 20 specimens of B. gouldingi, and their genetic parameters were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Cross-amplification; Molecular markers; SSR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100406

Variabilidade genética entre acessos de açaizeiro utilizando marcadores microssatélites
Provedor de dados: 64
Autores: Oliveira,Maria do Socorro Padilha de; Santos,João Bosco dos; Amorim,Edson Perito; Ferreira,Daniel Furtado.
Conduziu-se este trabalho, com o objetivo de caracterizar a variabilidade genética entre 116 acessos de açaizeiro da coleção de germoplasma da Embrapa Amazônia Oriental por marcadores microssatélites (SSR). As reações foram efetuadas com base em 116 amostras de DNA, utilizando sete primers SSR. Os níveis de polimorfismo e as estimativas das distâncias genéticas de Roger foram determinados pelas frequências alélicas e agrupado pelo método UPGMA. Os sete locos SSR revelaram 42 alelos com média de 6 alelos por loco. O conteúdo de informação de polimorfismo (PIC) variou de 0,60 a 0,86 com média de 0,75 e as heterozigosidades observada (Ho) e esperada (He) foram de 0,54 e 0,75, respectivamente. A distância genética média entre todos os acessos foi de 0,61,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Euterpe oleracea; Germoplasma; Polimorfismo; Diversidade genética; SSR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000500025

Identificação de variantes somaclonais em bananeiras 'Prata Anã', utilizando técnicas moleculares e citogenéticas
Provedor de dados: 64
Autores: Guimarães,Nilson César Castanheira; Torga,Paula Pereira; Resende,Eliane Cristina de; Chalfun Júnior,Antônio; Paiva,Edílson; Paiva,Luciano Vilela.
Variação somaclonal é uma variação fenotípica de origem genética, ou seja, uma variação cromossômica que se torna herdável nas gerações seguintes, ou epigenética, que é uma variação transitória devido ao estresse fisiológico que o material sofre, quando submetido ao cultivo in vitro. Um problema específico envolvendo a variação somaclonal em bananeiras 'Prata Anã' foi observado em Andradas, Minas Gerais, em plantas oriundas de micropropagação. A maior dificuldade na separação dos indivíduos normais e variantes é que os caracteres morfológicos, que são inerentes a este tipo de variação, só se tornam evidentes quando a planta está adulta, o que impossibilita a eliminação dos indivíduos variantes ainda em viveiro. Com o objetivo de identificar, ainda em...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Variação somaclonal; Bananeira; SSR; RAPD; Citogenética.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542009000200013