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Molecular investigation of tRNA genes integrity and its relation to pathogenicity islands in Shiga toxin-producing Escherichia coli (STEC) strains
Provedor de dados: 74
Autores: Novais,Rogério Carlos; Chaves,Marcela Cassin; Gonzalez,Alice Gonçalves Martins; Andrade,João Ramos Costa.
tRNA genes are known target sites for the integration of pathogenicity islands (PAI) and other genetic elements, such as bacteriophages, into bacterial genome. In most STEC (Shiga toxin-producing Escherichia coli), the PAI called LEE (locus of enterocyte effacement) is related to bacterial virulence and is mostly associated to the tRNA genes selC and pheU. In this work, we first investigated the relationship of LEE with tRNA genes selC and pheU in 43 STEC strains. We found that 28 strains (65%) had a disrupted selC and/or pheU. Three of these strains (637/1, 650/5 and 654/3) were chosen to be submitted to a RAPD-PCR technique modified by the introduction of specific primers (corresponding to the 5'end of genes selC and pheU) into the reaction, which we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pathogenicity islands; TRNA; STEC; RAPD-PCR; E.coli.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400020

Prevalence and Characterization of Shiga Toxin-Producing and Enteropathogenic Escherichia coli in Shellfish-Harvesting Areas and Their Watersheds
Provedor de dados: 5
Autores: Baliere, Charlotte; Rince, Alain; Blanco, Jorge; Dahbi, Ghizlane; Harei, Josee; Vogeleer, Philippe; Giard, Jean-christophe; Mariani-kurkdfian-, Patricia; Gourmelon, Michele.
Tipo: Text
Palavras-chave: STEC; EPEC; Shellfish; Water; MLST; PFGE; Biofilms.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00298/40904/39949.pdf

Les Escherichia Coli potentiellement pathogenes dans l’environnement littoral : cas des STEC et des EPEC
Provedor de dados: 5
Autores: Baliere, Charlotte.
The contamination of coastal areas by potentially pathogenic enteric bacteria is of concern for the sustainability of some uses, such as shellfish farming, recreational shellfish harvesting and bathing. The contamination of these environments may occur through the land-spreading of livestock wastes, animal feces deposited on pastures, wastewaters from slaughterhouses or from municipal wastewater treatment plants. The presence of these bacteria in coastal environment may present a potential risk to human health. In fact, shellfish-borne outbreaks or human infections may occur by the consumption of shellfish from contaminated areas or the ingestion of water during bathing in contaminated waters, respectively. To date, few studies focusing on the presence and...
Tipo: Text
Palavras-chave: STEC; EPEC; Coquillages; Diversité; Virulence; Persistance; STEC; EPEC; Shellfish; Diversity; Virulence; Persistence.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00312/42322/41653.pdf

Fecal contamination in coastal areas: An engineering approach
Provedor de dados: 5
Autores: Pommepuy, Monique; Hervio Heath, Dominique; Caprais, Marie-paule; Gourmelon, Michele; Le Saux, Jean-claude; Le Guyader, Soizick.
Introduction : "The occurrence of pathogenic microorganisms in seawater or in shellfish could exist anytime sewage from human or animal origin would be discharged to the coast" (Metcalf, 1982). According to the diseases occurring in the human population or in animals, pathogens might be present in recreational waters or in shellfish. Thus, the presence of human enteric viruses (norovirus, astrovirus, rotavirus, hepatitisAvirus (HAV)) and pathogenic bacteria (Salmonella, Listeria monocytogenes, Shiga-toxin-producing Escherichia coli (STEC), Vibrio cholerae, Vibrio parahaemolyticus, etc.) has been reported in coastal areas for a long time (Colwell, 1978; Metcalf, 1978; Melnick et al., 1979; Grimes, 1991; Bosch et al., 2001;Kong et al., 2002). These...
Tipo: Text
Palavras-chave: Estimating model; Infection dose; Microorganisms; STEC; Bacteria; Virus; Fecal contamination; Pathogenic agent; Marine sediment; Estuarine water; Coastal area; Marine water.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1207.pdf

Factors Involved in the Persistence of a Shiga Toxin-Producing Escherichia coil O157:H7 Strain in Bovine Feces and Gastro-Intestinal Content
Provedor de dados: 5
Autores: Segura, Audrey; Auffret, Pauline; Bibbal, Delphine; Bertoni, Marine; Durand, Alexandra; Jubelin, Gregory; Kerouredan, Monique; Brugere, Hubert; Bertin, Yolande; Forano, Evelyne.
Healthy cattle are the primary reservoir for O157:H7 Shiga toxin-producing E coil responsible for human food-borne infections. Because farm environment acts as a source of cattle contamination, it is important to better understand the factors controlling the persistence of E. coil O157:H7 outside the bovine gut. The E. coil O157:H7 strain MC2, identified as a persistent strain in French farms, possessed the characteristics required to cause human infections and genetic markers associated with clinical O157:H7 isolates. Therefore, the capacity of E. coil MC2 to survive during its transit through the bovine gastro-intestinal tract (GIT) and to respond to stresses potentially encountered in extra intestinal environments was analyzed. E. coil MC2 survived in...
Tipo: Text
Palavras-chave: Escherichia coli; STEC; EHEC; O157:H7; Biofilms; Stress response; Persistence.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00434/54588/55972.pdf

Factores de riesgo de infección por cepas de Escherichia coli shigatoxigénicas en gatos y perros
Provedor de dados: 78
Autores: Bentancor,A; Agostini,A; Rumi,M.V; Degregorio,O.J.
El Síndrome Urémico Hemolítico causado por Escherichia coli shigatoxigénico, STEC, es endémico en Argentina. Si bien es una enfermedad de Transmisión Alimentaria, se postula que el contagio persona-persona tiene alto impacto en nuestro país. Dada la estrecha relación personasanimales de compañía en los centros urbanos, esta ruta ha sido evaluada. El objetivo del trabajo es comunicar los factores de riesgo asociados a la portación-infección por STEC en animales de compañía de Buenos Aires, Argentina, proporcionando información útil para evaluar el riesgo de transmisión de este patógeno. Se recolectaron hisopados rectales de 149 gatos y 450 perros desde abril de 2005 a marzo de 2006. Se elaboró una ficha epidemiológica para cada animal. Se realizó...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: STEC; Síndrome Urémico Hemolítico; Factores de riesgo; Gatos; Perros.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982008000100001

Contaminación de carne molida con cepas de Escherichia coli shigatoxigénico (STEC) provenientes de comercios minoristas de San Martín, Buenos Aires, categorizados según nivel socioeconómico
Provedor de dados: 78
Autores: Miccio,L; Rumi,M.V; Llorente,P; Bentancor,A.B.
Escherichia coli shigatoxigénico (STEC) produce enfermedades de transmisión alimentaria, desde diarreas leves a Síndrome Urémico Hemolítico, enfermedad de impacto en Argentina. Dentro de los alimentos implicados se destaca la carne bovina insuficientemente cocida. La contaminación de la carne molida y su relación con los estratos socioeconómicos no ha sido estudiada. El objetivo de este trabajo fue identificar en muestras de carne cepas STEC y establecer su perfil de virulencia, considerando la zona socioeconómica de procedencia y la persistencia de la contaminación en el local de venta. Se seleccionó el 30% de las bocas de expendio habilitadas en cada nivel socioeconómico. Se analizaron 72 muestras provenientes de 36 carnicerías de San Martín, Buenos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: STEC; Síndrome Urémico Hemolítico; Carne; Enfermedad transmitida por alimentos; Nivel socioeconómico.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982011000100004

Escherichia coli in Chicken Carcasses in Southern Brazil: Absence of Shigatoxigenic (STEC) and Isolation of Atypical Enteropathogenic (aEPEC)
Provedor de dados: 89
Autores: Cerutti,MF; Vieira,TR; Zenato,KS; Werlang,GO; Pissetti,C; Cardoso,M.
ABSTRACT The aim of this study was to investigate the presence of shiga toxin-producing Escherichia coli (STEC) and atypical enteropathogenic Escherichia coli (aEPEC) in frozen chicken carcasses sold at stores in southern Brazil. Typical E. coli colonies were enumerated in 246 chicken carcasses, and the presence of stx1, stx2, eae genes was investigated in their rinse liquid and in E. coli strains isolated from those carcasses. Strains of E. coli were also investigated for the presence of bfp gene. A median of 0.6 cfu.g-1(ranging from &lt;0.1 to 242.7 cfu.g-1) of typical E. coli colonies was found in the carcasses. Shiga toxin-encoding genes (stx1 and stx2) were not detected, indicating that the chicken carcasses were negative for STEC. The intimin protein...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken meat; Enteropathogenic Escherichia coli; EPEC; Shigatoxigenic Escherichia coli; STEC.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100310

Prevalence of Shiga Toxin-Producing and Enteropathogenic Escherichia coli in Wild and Pet Birds in Iran
Provedor de dados: 89
Autores: Koochakzadeh,A; Askari Badouei,M; Zahraei Salehi,T; Aghasharif,S; Soltani,M; Ehsan,MR.
ABSTRACT The aim of this study was to investigate the prevalence of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) strains and to identify the stx gene types in wild captive and companion birds. In total,657 E. coli isolates from 219 birds belonging to 38 different species were investigated for the presence of STEC and EPEC strains. It was shown that five birds (2.28%) carried strains positive for one or more of the virulence factors investigated. The results indicated that 1.8% (n=4) and 0.45% (n=1) of the birds carried STEC and EPEC strains, respectively. All STEC strains harbored the stx2f and eae genes and this finding reveals the role of other birds, in addition to pigeons, as reservoirs of STEC. The only EPEC strain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EPEC; STEC; Stx2f; Pet birds; Wild birds.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000400445

Caracterização molecular e fenotípica de estirpes de Escherichia coli produtoras de shiga-toxina (STEC) não-O157 de fezes e carcaças bovinas
Provedor de dados: 45
Autores: Carvalho,A.F.; Miyashiro,S.; Nassar,A.F.C.; Noda,A.; Gabriel,D.T.; Baldassi,L.
Foram coletados 100 suabes retais e 100 suabes de carcaças bovinas em matadouros do estado de São Paulo, e um total de 326 estirpes de E. coli foram identificadas, sendo 163 de amostras retais e 163 de amostras de carcaça. Todos os isolados submetidos à PCR para detecção dos genes das toxinas Stx1 e Stx2 foram identificados como não-O157 e fenotipados pelo teste da citotoxicidade em células Vero. Das 26 estirpes que apresentaram apenas o gene stx1, das 56 que apresentaram apenas o gene stx2 e das 30 estirpes que apresentaram ambos os genes, 17 (65,4%), 42 (75%) e 22 (73,3%), respectivamente, foram positivas ao teste de citotoxicidade. Não houve diferença estatística entre os três perfis genéticos e na positividade ao teste de citotoxicidade. Os resultados...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Escherichia coli; STEC; Fezes; Carcaça.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400014

Virulence genes in Escherichia coli isolated from feces and urine of cats
Provedor de dados: 45
Autores: Caliman,M.W.; Marin,J.M.
Determinadas linhagens de Escherichia coli comportam-se como patógenos em gatos, causando doenças gastrointestinais e extraintestinais. Neste estudo, foram utilizadas 205 cepas de E. coli isoladas de amostras de fezes provenientes de 19 gatos diarreicos e de 21 gatos não diarreicos, e três amostras de urina provenientes de gatos com infecção do trato urinário (ITU). Essas cepas foram testadas pela técnica de reação em cadeia da polimerase com múltiplos iniciadores para a detecção da presença de genes codificadores de adesinas (pap, sfa e afa), assim como para a detecção dos genes produtores da toxina Shiga-like (stx1 e stx2) e do gene da intimina (eae). Oitenta e dois isolados possuíam genes codificadores de adesinas, dos quais 11 apresentaram o gene pap,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pet; Cat; ExPEC; STEC; Adhesins; Virulence gene.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000401287

Fate of non O157 Shiga toxigenic Escherichia coli in composted cattle manure
Provedor de dados: 45
Autores: Gonçalves,V.P.; Marin,J.M.
To determine the fate of Shiga toxigenic Escherichia coli (STEC) non-O 157 in composted manure from naturally colonized cattle, fresh manure was obtained from three cows carrying non-O157 STEC strains possessing the stx2 gene. Two composting systems were used: a 0.6m deep cave opened in the soil and an one meter high solid manure heap in a pyramidal architecture. Every day, for the 10 first days, and every five days for a month, one manure sample from three different points in both systems was collected and cultured to determine the presence of E. coli and the presence of the stx 2 gene in the cells. The temperature was verified at each sampling. STEC non-O157 E. coli cells survived for 8, 25 and 30 days at 42, 40 and 38ºC, respectively, in the deep cave...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial reduction; Manure; STEC; Environment; Composting system.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000400001

Isolation of Shiga toxigenic Escherichia coli from butcheries in Taquaritinga city, State of São Paulo, Brazil
Provedor de dados: 58
Autores: Rodolpho,Daniela; Marin,José Moacir.
Shiga toxigenic Escherichia coli (STEC) has been implicated as the cause of several human diseases. Samples (ground beef, grinding-machines and human hands) from 23 butcheries were assayed for E. coli using standard microbiological methods, and 287 isolates were submitted to polymerase chain reaction for the detection of stx 1, stx 2 and eae genes. Four STEC isolates were recovered, two from ground beef and two from grinding-machines; all harbored the stx 2 gene and were negative for the eae gene. All E. coli isolates including the four STEC were screened for antibiotic resistance. High levels of resistance against several antimicrobial agents were detected; those most commonly observed were to tetracycline (76.6%), amoxicillin (64.1%) and cephalothin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; STEC; Stx 2; Antibiotic resistance; Ground beef; Cattle.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400004

Captive wild birds as reservoirs of enteropathogenic E. coli (EPEC) and Shiga-toxin producing E. coli (STEC)
Provedor de dados: 58
Autores: Sanches,Lilian Aparecida; Gomes,Marcelo da Silva; Teixeira,Rodrigo Hidalgo Friciello; Cunha,Marcos Paulo Vieira; Oliveira,Maria Gabriela Xavier de; Vieira,Mônica Aparecida Midolli; Gomes,Tânia Aparecida Tardelli; Knobl,Terezinha.
ABSTRACT Psittacine birds have been identified as reservoirs of diarrheagenic Escherichia coli, a subset of pathogens associated with mortality of children in tropical countries. The role of other orders of birds as source of infection is unclear. The aim of this study was to perform the molecular diagnosis of infection with diarrheagenic E. coli in 10 different orders of captive wild birds in the state of São Paulo, Brazil. Fecal samples were analyzed from 516 birds belonging to 10 orders: Accipitriformes, Anseriformes, Columbiformes, Falconiformes, Galliformes, Passeriformes, Pelecaniformes, Piciformes, Psittaciformes and Strigiformes. After isolation, 401 E. coli strains were subjected to multiplex PCR system with amplification of genes eae and bfp...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diarrheagenic E. coli; Atypical EPEC; STEC; Avian diseases.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400760

Shigatoxigenic and atypical enteropathogenic Escherichia coli in fish for human consumption
Provedor de dados: 58
Autores: Cardozo,Marita Vedovelli; Borges,Clarissa Araújo; Beraldo,Lívia Gerbasi; Maluta,Renato Pariz; Pollo,Andressa Souza; Borzi,Mariana Monezi; dos Santos,Luis Fernando; Kariyawasam,Subhashinie; Ávila,Fernando Antônio de.
ABSTRACT Shigatoxigenic and enteropathogenic Escherichia coli with virulence and multidrug resistance profile were isolated from Nile tilapia. This study finding is of great importance to public health because they help understand this pathogen epidemiology in fish and demonstrate how these animals can transmit E. coli related diseases to humans.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: EPEC; Fish; MLST; STEC; Virulence genes.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400936

Prevalence and characteristics of Shiga toxin-producing Escherichia coli (STEC) strains in ground beef in São Paulo, Brazil
Provedor de dados: 58
Autores: Bergamini,Alzira Maria Morato; Simões,Marise; Irino,Kinue; Gomes,Tânia Aparecida Tardelli; Guth,Beatriz Ernestina Cabilio.
This study aims to assess the prevalence of Shiga toxin-producing Escherichia coli (STEC) in ground beef collected in two cities located in the State of São Paulo, Brazil. A total of 250 samples of raw ground beef were collected in local grocery stores during the period of March to December 2002 in the cities of Ribeirão Preto (114 samples) and Campinas (136 samples), São Paulo State, Brazil. The samples were processed according to standard methods. The resulting 591 E.coli colonies were screened for STEC by hybridization assays using the specific DNA probes, stx1,stx2 and eae. Further characterization of STEC isolates included the search for the ehxA sequence, detection of enterohemolysin and expression of Shiga toxin using the Vero cell assay. STEC...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Shiga toxin; STEC; Serotypes; Ground beef.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300032

Antibiotic resistance and integrons in Shiga toxin-producing Escherichia coli (STEC)
Provedor de dados: 58
Autores: Colello,Rocío; Etcheverría,Analía I.; Conza,Jose A. Di; Gutkind,Gabriel O.; Padola,Nora L.
Shiga toxin-producing Escherichia coli (STEC) cause hemorrhagic colitis (HC) and hemolytic-uremic syndrome in humans (HUS). Cattle are the main reservoir of STEC and transmission to humans occurs through contaminated food and water. Antibiotics are used in pig production systems to combat disease and improve productivity and play a key role in the dissemination of antibiotic resistance genes to the bacteria. Integrons have been identified in resistant bacteria allowing for the acquisition and dissemination of antibiotic resistance genes. STEC strains isolated from humans and animals have developed antibiotic resistance. In our laboratory, 21 non-157 STEC strains isolated from pigs were analyzed to detect class 1 and 2 integrons by PCR. Eight carried...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: STEC; Integrons; Multiresistance; Antibiotics; Public health.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100001

Isolation of Shiga toxin-producing Escherichia coli (STEC) serotype 091:H21 from a child with diarrhea in Porto Alegre city, RS, Brazil
Provedor de dados: 58
Autores: Cantarelli,Vlademir; Nagayama,Kenichi; Takahashi,Akira; Honda,Takeshi; Cauduro,Paulo F.; Dias,Cícero A.G.; Mezzari,Adelina; Brodt,Teresa.
We describe the isolation of one strain of Shiga toxin-producing Escherichia coli O91:H21 from a child with diarrhea in the city of Porto Alegre, RS, Brazil. Considering the pathogenic potential of STEC, these organisms should be looked for more carefully among our population.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Enterohemorrhagic E. coli; STEC; Enteroaggregative E. coli; Enteropathogenic E. coli; Diarrhea.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400005

Survey of Minas frescal cheese from Southwest Minas Gerais for virulence factors and antimicrobial resistance in Escherichia coli isolates
Provedor de dados: 65
Autores: Okura,Mõnica Hitomi; Marin,José Moacir.
The soft cheese Minas frescal is one of the most popular cheese in Brazil, which is typically manufactured in small dairy farms under unsatisfactory hygiene conditions. To assess the risk involved in consumption of this cheese, virulence markers were investigated in 330 Escherichia coli strains isolated from 30 Minas frescal cheeses inspected by official government agency (SIF - serviço de inspeção federal), from 50 cheeses not inspected by SIF and 31 cheeses not inspected by SIF with spice added, all of them collected in the southwest of Minas Gerais State. The E. coli isolates were screened for the presence of Shiga toxin-encoding (stx 1 and stx 2), intimin (eae) genes and for the presence of (pap, sfa, afa) genes related to adhesion in epithelial cells....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Cheese; STEC; ExPEC; Antimicrobial resistance; Minas frescal.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000801506

Occurrence of non-O157 Shiga toxin-producing Escherichia coli in dogs with diarrhea
Provedor de dados: 65
Autores: Paula,Cleber Jacob Silva de; Marin,José Moacir.
Shiga toxigenic Escherichia coli (STEC) and Attaching and effacing E. coli (AEEC) have been associated with diarrhea illness in dogs. From January to December 2006, 92 E. coli isolates from 25 diarrheic dogs were analyzed, by screening for the presence of Shiga toxin-producing (stx 1 and stx 2) and intimin (eae) genes. Twelve isolates were detected by PCR to harbor the Shiga toxin genes (7 the stx 1 (7.6%); 5 the stx 2 (5.4%); and none both of them). Nine (9.8%) of the E. coli isolates studied were eae positive non Shiga toxin-producing. Thirteen (62.0%) isolates, carrying stx or eae gene, also showed a hemolysin production. The strains with virulence genes were also examined for resistance to 12 antimicrobial agents. Resistances to cephalothin (85.7%),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; STEC; Eae gene; Antimicrobial resistance.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000600029