Consulta: subjectFacets:"Sarcoplasmic reticulum calcium ATPase"
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Data/hora: 03/06/2024 06:22:04

Inhibition of the sarcoplasmic reticulum Ca2+ pump with thapsigargin to estimate the contribution of Na+-Ca2+ exchange to ventricular myocyte relaxation
Provedor de dados: 56
Autores: Bassani,R.A.; Bassani,J.W.M.
Relaxation in the mammalian ventricle is initiated by Ca2+ removal from the cytosol, which is performed by three main transport systems: sarcoplasmic reticulum Ca2+-ATPase (SR-A), Na+-Ca2+ exchanger (NCX) and the so-called slow mechanisms (sarcolemmal Ca2+-ATPase and mitochondrial Ca2+ uptake). To estimate the relative contribution of each system to twitch relaxation, SR Ca2+ accumulation must be selectively inhibited, usually by the application of high caffeine concentrations. However, caffeine has been reported to often cause changes in membrane potential due to NCX-generated inward current, which compromises the reliability of its use. In the present study, we estimated integrated Ca2+ fluxes carried by SR-A, NCX and slow mechanisms during twitch...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Myocardium; Relaxation; Calcium fluxes; Sodium-calcium exchanger; Sarcoplasmic reticulum calcium ATPase; Caffeine.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200014