Consulta: subjectFacets:"Scanning electron microscopy"
Registros recuperados: 73
Data/hora: 01/06/2024 04:47:29

Cytokinin preconditioning enhances multiple shoot regeneration in Pongamia pinnata (L.) Pierre - a potential, non-edible tree seed oil source for biodiesel
Provedor de dados: 69
Autores: Belide,Srinivas; Sajjalaguddam,Ramgopal Rao; Paladugu,Anitha.
An efficient, highly reproducible protocol for multiple shoot induction and plant regeneration of Pongamia pinnata has been successfully developed using cotyledonary node explants. This study also demonstrates that preconditioning of explant stimulates production of multiple shoots from cotyledonary nodes of P. pinnata. The highest direct shoot regeneration (90%) with an average of 18.4 ï¿½ 3.1 shoots/explant were obtained when cotyledonary node explants were excised from seedlings germinated on Murashige and Skoog (MS) media supplemented with benzyladenine (BA) 1 mg l-1, and subsequently cultured on MS media with 1 mgl-1 thidiazuron (TDZ). Scanning electron microscope observations of cotyledonary node (CN) explants excised from pre-conditioned and normal...
Tipo: Journal article
Palavras-chave: Benzyladenine; Cotyledonary node; Multiple shoot induction; Scanning electron microscopy; Thidiazuron.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600003

Micropropagation of Homalomena pineodora Sulaiman &amp;amp; Boyce (Araceae): a new species from Malaysia
Provedor de dados: 75
Autores: Stanly,Christine; Bhatt,Arvind; Sulaiman,Baharuddin; Keng,Chan Lai.
Homalomena pineodora (family Araceae) is a species found to have impressive foliage characteristics which remain evergreen throughout the year. Therefore, H. pineodora can be grown as an ornamental plant. Generally H. pineodora needs 3-5 years to propagate and multiply. However, the demand for new ornamental plants is increasing worldwide and the quality of planting material is a basic need for boosting productivity. Therefore an efficient micropropagation protocol for large-scale production of H. pineodora was developed. In vitro shoot cultures were initiated from the rhizomatous buds on MS basal medium. The best conditions for propagating H. pineodora was found to be MS medium supplemented with 3% sucrose and 0.5 mg L-1 BA (6-benzyladenine) under 24 h of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Homalomena pineodora; Acclimatization; Scanning electron microscopy; Cytokinin; In vitro culture; Shoot proliferation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362012000100007

Updating Geographic Distribution of Artemia urmiana GÜNTHER,    1890 (Branchiopoda: Anostraca) in Europe: An Integrated and Interdisciplinary Approach
Provedor de dados: 13
Autores: Abatzopoulos, Th. J.; Amat, F.; Baxevanis, A. D.; Belmonte, G.; Hontoria, F.; Maniatsi, S.; Moscatello, S.; Mura, G.; Shadrin, N. V.
Artemia urmiana (a species previously considered endemic of Lake Urmia, NW Iran) has been found in Lake Koyashskoe, a hypersaline lake on the Black Sea coast of the Crimean peninsula (Ukraine). Therefore, this is the first record of A. urmiana in Europe which updates its distribution. The species identification was based on an integrated and interdisciplinary approach using discriminant analysis of the morphometric characters, scanning electron microscopy, and molecular profile analysis. The data derived from the above mentioned approaches converge to significant similarity of the population under investigation with A. urmiana. The updated geographic distribution of the species, deriving from the present report, asks for additional contribution of other...
Tipo: Journal Contribution
Palavras-chave: Salt lakes; Scanning electron microscopy; Discriminant analysis; Molecular analysis; Biogeography.
Ano: 2009
URL: http://repository.ibss.org.ua/dspace/handle/99011/1069

Etude du biofilm microalgal des vasières intertidales : dynamique spatio-temporelle à micro-échelle et performances photosynthétiques
Provedor de dados: 5
Autores: Herlory, Olivier.
The microalgal biofilm, which is present on sediment surface, is the primary production unit on bare intertidal mudlfats. In order to improve our understanding of this primary production system, a study was undertaken at microscale to analyse the structural and functional properties of this biofilm. In the first part of this work, a detailed descriptive analysis lead to the characterisation of the spatiotemporal dynamics of the physical structure of the biofilm. It turns out that the biofilm exhibits its own dynamics at the sediment surface (as a discrete compartment in the top 200 µm), but still has a functional relationship with the total available biomass (PCB) distributed within the top 1 cm (linear relationship between the maximum size of the biofilm...
Tipo: Text
Palavras-chave: PAM Fluorometry; Scanning electron microscopy; Photosynthesis; Spatio temporal dynamic; Micro scale; Microphytobenthic biofilm; Intertidal mudflats; Fluorimétrie PAM; Microscopie électronique à balayage; Photosynthèse; Dynamique spatio temporelle; Micro échelle; Biofilm microphytobenthique; Vasière intertidal.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/these-2138.pdf

Polystyrene microbeads modulate the energy metabolism of the marine diatom Chaetoceros neogracile
Provedor de dados: 5
Autores: Seoane, Marta; González-fernández, Carmen; Soudant, Philippe; Huvet, Arnaud; Esperanza, Marta; Cid, Ángeles; Paul-pont, Ika.
Due to the growing concern about the presence of microplastics (MP) in the environment, the number of studies evaluating the toxicity of these small persistent particles on different marine species has increased in recent years. Few studies have addressed their impact on marine phytoplankton, a subject of great concern since they are primary producers of the aquatic food web. The aim of this study is to unravel the cytotoxicity of 2.5 μg mL−1 unlabelled amino-modified polystyrene beads of different sizes (0.5 and 2 μm) on the marine diatom Chaetoceros neogracile. In addition to traditional growth and photosynthesis endpoints, several physiological and biochemical parameters were monitored every 24 h in C. neogracile cells by flow cytometry during their...
Tipo: Text
Palavras-chave: Microalgae; Microplastic; Flow cytometry; Cytotoxicity; Scanning electron microscopy.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00492/60415/63850.pdf

Mineral phase in shell repair of Manila clam Venerupis philippinarum affected by brown ring disease
Provedor de dados: 5
Autores: Trinkler, Nolwenn; Bardeau, Jean-francois; Marin, Frederic; Labonne, Maylis; Jolivet, Aurelie; Crassous, Philippe; Paillard, Christine.
The mineral phase of shell repair in the Manila clam Venerupis philippinarum affected by brown ring disease (BRD) was characterised at various scales and at various stages of shell repair by confocal Raman microspectrometry and scanning electron microscopy. Spherulitic and quadrangular aragonite microstructures associated with polyene pigments were clearly observed. Von Kossa staining showed that at the beginning of shell repair, hemocytes are filled with insoluble calcium carbonate salts in all fluids and then are transported toward the extrapallial fluids and the repair sites. Our analyses suggest that after a Vibrio tapetis attack and BRD deposit some clams rapidly cover the deposit, resulting in a modification in the microstructure, which could be...
Tipo: Text
Palavras-chave: Aragonite; Hemocytes; Shell microstructure; Calcium carbonate; Biomineralization; Mollusc; Raman microspectrometry; Scanning electron microscopy.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00029/14065/11301.pdf

Comparison of the physical and geotechnical properties of gas-hydrate-bearing sediments from offshore India and other gas-hydrate-reservoir systems
Provedor de dados: 5
Autores: Winters, W. J.; Wilcox-cline, R. W.; Long, P.; Dewri, S. K.; Kumar, P.; Stern, L.; Kerr, L.
The sediment characteristics of hydrate-bearing reservoirs profoundly affect the formation, distribution, and morphology of gas hydrate. The presence and type of gas, porewater chemistry, fluid migration, and subbottom temperature may govern the hydrate formation process, but it is the host sediment that commonly dictates final hydrate habit, and whether hydrate may be economically developed. In this paper, the physical properties of hydrate-bearing regions offshore eastern India (Krishna-Godavari and Mahanadi Basins) and the Andaman Islands, determined from Expedition NGHP-01 cores, are compared to each other, well logs, and published results of other hydrate reservoirs. Properties from the hydrate-free Kerala-Konkan basin off the west coast of India are...
Tipo: Text
Palavras-chave: Physical properties; Gas hydrate; Porosity; Atterberg limits; Consolidation; Permeability; Shear strength; Scanning electron microscopy.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00289/40036/38799.pdf

Recovery in culture of viable but nonculturable Vibrio parahaemolyticus: regrowth or resuscitation?
Provedor de dados: 5
Autores: Coutard, Francois; Crassous, Philippe; Droguet, Michael; Gobin, Eric; Colwell, Rita; Pommepuy, Monique; Hervio Heath, Dominique.
The objective of this study was to explore the recovery of culturability of viable but nonculturable ( VBNC) Vibrio parahaemolyticus after temperature upshift and to determine whether regrowth or resuscitation occurred. A clinical strain of V. parahaemolyticus Vp5 was rendered VBNC by exposure to artificial seawater ( ASW) at 4 degrees C. Aliquots of the ASW suspension of cells ( 0.1, 1 and 10 ml) were subjected to increased temperatures of 20 degrees C and 37 degrees C. Culturability of the cells in the aliquots was monitored for colony formation on a rich medium and changes in morphology were measured by scanning ( SEM) and transmission ( TEM) electron microscopy. Samples of VBNC cells were fixed and examined by SEM, revealing a heterogeneous population...
Tipo: Text
Palavras-chave: Transmission electron microscopy; Scanning electron microscopy; Resuscitation; Regrowth; Vibrio parahaemolyticus; VBNC state.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3117.pdf

Bioprotective Effect of Lactococcus piscium CNCM I-4031 Against Listeria monocytogenes Growth and Virulence
Provedor de dados: 5
Autores: Saraoui, Taous; Leroi, Francoise; Chevalier, Frederique; Cappelier, Jean-michel; Passerini, Delphine; Pilet, Marie-france.
Listeria monocytogenes is a Gram-positive pathogen occurring in many refrigerated ready-to-eat foods. It is responsible for foodborne listeriosis, a rare but severe disease with a high mortality rate (20–30%). Lactococcus piscium CNCM I-4031 has the capacity to prevent the growth of L. monocytogenes in contaminated peeled and cooked shrimp and in a chemically defined medium using a cell-to-cell contact-dependent mechanism. To characterize this inhibition further, the effect of L. piscium was tested on a collection of 42 L. monocytogenes strains. All strains were inhibited but had different sensitivities. The effect of the initial concentration of the protective and the target bacteria revealed that the inhibition always occurred when L. piscium had reached...
Tipo: Text
Palavras-chave: Biopreservation; Lactococcus piscium; Listeria monocytogenes; Co-culture; Cell ratio; Scanning electron microscopy; Virulence.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00449/56065/57588.pdf

Comparative Scanning Electron Microscopy of Platelets and Fibrin Networks of Human and Differents Animals
Provedor de dados: 77
Autores: Pretorius,E; Vieira,W. A; Oberholzer,H. M; Auer,R. E. J.
The purpose of the present study was to compare the platelet and fibrin network ultrastructure of humans to eight different animal species in order to determine the differences between human and animal platelet and fibrin morphology, and to determine whether the animals studied differ in their platelet and fibrin morphology, and whether these differences can be observed by scanning electron microscopy. Platelets and fibrin networks play an important role both in the coagulation process as well as physiologically in allergic processes and immunological mechanisms. The thickness of human fibrin networks were compared to mouse (Mus musculus), equine (Equus caballus), vervet monkey (Chlorocebus aethiops previously Cercopithecus aethiops), oryx (Oryx gazella),...
Tipo: Journal article
Palavras-chave: Scanning electron microscopy; Platelets; Fibrin networks; Human; Animals.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000100013

Preservation of Haemal Nodes Fixed by Means of Two Different Aldehyde Fixatives: a Scanning Electron Microscopic Study
Provedor de dados: 77
Autores: Guerrero,F; Cerutti,P; Marcaccini,A.
Glutaraldehyde is the fixative most commonly used in electron microscope studies of biological tissues, however it is often necessary to use samples which were not fixed in this fixative, even with the usual uncertainty of the results that may be obtained. The fixation is the more delicate step of the sample processing. Therefore in this work, the quality of preservation of haemal nodes fixed with two classic aldehyde fixatives: formaldehyde and glutaraldehyde we have compared under the scanning electron microscope. Our results showed that both fixatives were successful in preserving the morphology of haemal nodes components; however glutaraldehyde conferred satisfactory results mainly in the preservation of parenchymal cells, whereas formaldehyde was...
Tipo: Journal article
Palavras-chave: Scanning electron microscopy; Fixation; Fixative; Haemal node; Glutaraldehyde; Formaldehyde.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200059

CHORDAE TENDINEAE ARCHITECTURE IN THE PAPILLARY MUSCLE INSERTION
Provedor de dados: 77
Autores: Wang Gusukuma,Luciana; Prates,José Carlos; Smith,Ricardo Luiz.
Chordae tendineae architecture and their junction with papillary muscle were studied with scanning electron microscopy, comparing human and swine hearts. Papillary muscles with the corresponding chordae tendineae were obtained from human and pig (Sus scrofa) hearts after exposition of the left ventricle cavity, fixed with 4% formaldehyde. The specimens were sectioned in small samples and divided in four groups, a control one and the others submitted to digestion in a solution of 2.5% sodium hypochlorite in saline solution during 5, 10 and 15 minutes respectively. The specimens were washed and fixed in 1% osmium tetroxide in cacodylate buffer, kept in 1% tannic acid for 30 minutes, dehydrated and critical point dried, mounted and sputtered with gold. The...
Tipo: Journal article
Palavras-chave: Chordae tendineae; Architecture; Human; Pigs; Scanning electron microscopy.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000400005

Scanning Electron Microscopy of the Gastrointestinal Tract of Nile Perch (Lates niloticus, Linneaus, 1758)
Provedor de dados: 77
Autores: Namulawa,Victoria Tibenda; Kato,Charcles Drago; Nyatia,Edward; Rutaisire,Justus; Britz,Peter.
The morphology of the gastrointestinal tract of adult Nile perch was described using standard SEM procedures. Investigations revealed the presence of cardiform teeth in the oral cavity, goblet cells and finger print-like microridges on the hard palate and oesophagus lumenal surface. Elaborate patterns and bacterial cells were observed on the stomach lumenal surface and intense foldings in the intestinal region. These observations provide a better understanding of the morphology of the gut in Nile perch and how it is suited for its digestive function.
Tipo: Journal article
Palavras-chave: Gastrointestinal tract; Lates niloticus; Scanning electron microscopy.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300047

Three-dimensional Characteristics of the Interface Epithelium-Connective Tissue Surface of Fingeres Lamina Propria of Cebus apella Monkey: Scanning Electron Microscopy Study
Provedor de dados: 77
Autores: Watanabe,Ii-sei; Guimarães,Juliana Plácido; Iyomasa,Mamie Misuzaki; Miglino,Maria Angélica; Lopes,Marília Gabriela de Oliveira; Consentino,Márcia; Sosthines,Kronka; da Silva,Marcelo Cavenaghi Pereira.
In the present paper were analysed the three-dimensional characteristics of the interface epithelium-connective tissue surface of finger prints of Cebus apella monkey employing the scanning electrón microscopic methods. The connective tissue core (CTC) and epithelial papillae were examined verifying the three-dimensional configuration of the tissue projections. The samples were fixed in Bouin solsution for histologic preparations and in modified Karnovsky for examine to observe in scanning electrón microscopy. After treatment in the 10% NaOH solution during 3 to 5 day s, the surface of finger prints revealed a distribution of CTC of lamina propria in situ showing original three-dimensional SEM images. The linear and circular dispositions CTC, and the...
Tipo: Journal article
Palavras-chave: Skin; Finger; Collagen fibers; Cebus apella; Scanning electron microscopy.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000200013

The Carpal Joint of the Donkey (Equus asinus): Morphological Investigation
Provedor de dados: 77
Autores: Alsafy,Mohamed A. M; El-Gendy,Samir A.A; Abou-Ahmed,Howaida M.
The current study has been achieved to be an essential resource for all veterinary practitioners that deal with the anatomy of the carpal joint of the donkey. Ten adult donkeys of both sexes were used in the current study. The topographical approach to the carpal joint was investigated in this study. Radiography and computed tomography (CT) of the carpus delineated the articulations of the carpal joint: radiocarpal, intercarpal, and carpometacarpal. The carpal ligaments were well delineated and the carpal canal was demonstrated with its content such as superficial digital flexor tendon (SDFT) and deep digital flexor tendon (DDFT). The contrast radiography visualized that the radiocarpal joint outpouched proximal to the accessory carpal bone by large...
Tipo: Journal article
Palavras-chave: Carpal joint; Donkey; Radiography; Computed tomography; Scanning electron microscopy.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300023

Gill Morphology in Two Bottom Feeder Mediterranean Sea Fishes: Grey Gurnard Fish (Eutrigla gurnardus, Linnaeus, 1758) and Striped Red Mullet Fish (Mullus barbatus surmuletus, Linnaeus, 1758) by Scanning Electron Microscopy
Provedor de dados: 77
Autores: Abumandour,Mohamed M. A; El-Bakary,Neveen E. R.
The purpose of the current investigation was to describe the gill morphology of two bottom feeders Mediterranean Sea fishes: Striped red mullet fish (M. surmuletus) and grey gurnard fish (E. gurnardus) that showed the same feeding habits. The gill system of the grey gurnard consisted of four pairs of gill arches while consisted of three pairs of gill arches in the striped red mullet. Our study focused on the scanning electron microscopical (SEM) features of the gills, where some differences in the gill arches and gill rakers surface in both species were observed. Our results marked that there was an interbranchial septum carrying a four transverse elevated crest in grey gurnard while in striped red mullet carrying a median longitudinal elevated crest....
Tipo: Journal article
Palavras-chave: Grey gurnard fish; Striped red mullet fish; Scanning electron microscopy; Gill raker; Gill arch; Gill filament.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100014

Vestured pits: a diagnostic character in the secondary xylem of Myrtales
Provedor de dados: 16
Autores: Jansen, S.; Pletsers, A.; Rabaey, D.; Lens, F.
Vestures are small projections from the secondary cell wall associated with tracheary elements of the secondary xylem. They are usually associated with bordered pits and characterize various angiosperm families, including important timber species such as Dipterocarpaceae and Eucalyptus trees. The micromorphology and distribution of vestures were studied in 22 species representing all families within the order Myrtales based on light and scanning electron microscopy. Vestures are consistently present near the outer pit aperture of bordered vessel pits, suggesting the synapomorphic character of this feature for the entire order. It is unclear in which geological period this feature originated in the evolution of the pre-Myrtalean lineages. In some species...
Tipo: Article / Letter to the editor
Palavras-chave: Vestures; Wood anatomy; Bordered pits; Myrtales; Scanning electron microscopy; Light microscopy; Tracheary elements; 42.48; 42.56.
Ano: 2008
URL: http://www.repository.naturalis.nl/record/498037

The phylogenetic significance of vestured pits in Boraginaceae
Provedor de dados: 16
Autores: Rabaey, D.; Lens, F.; Smets, E.; Jansen, S.
The bordered pit structure in tracheary elements of 105 Boraginaceae species is studied using scanning electron microscopy to examine the systematic distribution of vestured pits. Forty-three species out of 16 genera show a uniform presence of this feature throughout their secondary xylem. Most vestures are small, unbranched and associated with the outer pit aperture of bordered intervessel pits. The feature is likely to have originated independently in the distantly related subfamilies Boraginoideae (tribe Lithospermeae) and Ehretioideae. The distribution of vestures in Ehretia agrees with recent molecular phylogenies: (1) species with vestured pits characterise the Ehretia I group (incl. Rotula), and (2) species with non-vestured pits belong to the...
Tipo: Article / Letter to the editor
Palavras-chave: Boraginaceae; Boraginoideae; Bordered pit; Ehretioideae; Scanning electron microscopy; Vestures; 42.56.
Ano: 2010
URL: http://www.repository.naturalis.nl/record/430079

Sodiomyces alkalinus, a new holomorphic alkaliphilic ascomycete within the Plectosphaerellaceae
Provedor de dados: 16
Autores: Grum-Grzhimaylo, A.A.; Debets, A.J.M.; Diepeningen, A.D. van; Georgieva, M.L.; Bilanenko, E.N.
In this study we reassess the taxonomic reference of the previously described holomorphic alkaliphilic fungus Heleococcum alkalinum isolated from soda soils in Russia, Mongolia and Tanzania. We show that it is not an actual member of the genus Heleococcum (order Hypocreales) as stated before and should, therefore, be excluded from it and renamed. Multi-locus gene phylogeny analyses (based on nuclear ITS, 5.8S rDNA, 28S rDNA, 18S rDNA, RPB2 and TEF1-alpha) have displayed this fungus as a new taxon at the genus level within the family Plectosphaerellaceae, Hypocreomycetidae, Ascomycota. The reference species of actual Heleococcum members showed clear divergence from the strongly supported Heleococcum alkalinum position within the Plectosphaerellaceae, sister...
Tipo: Article / Letter to the editor
Palavras-chave: Alkaliphilic fungi; Growth; Heleococcum alkalinum; Molecular phylogeny; Scanning electron microscopy; Taxonomy.
Ano: 2013
URL: http://www.repository.naturalis.nl/record/532154

Micromorphology in Mycale taxonomy (Mycalidae, Poecilosclerida, Demospongiae), with the description of two new micracanthoxea-bearing species
Provedor de dados: 16
Autores: Carballo, José Luis; Hajdu, Eduardo.
Two new species of Mycale with micracanthoxeas are described: Mycale (Aegogropila) lilianae n. sp., mainly characterized by the presence of toxas as large as 300 µm, and Mycale (Carmia) urizae n. sp., the first Mycale with micracanthoxeas described from Africa, characterized by the presence of anisochelae-III with the falx projecting downwards from the middle basal portion of the frontal alae of the head, and by the presence of two morphologically distinct size classes of toxas, with toxas-II being notably oxea-shaped. A discussion of all species of Mycale with micracanthoxeas is included. It is postulated that a comprehensive assessment of the distribution of this character, as well as of other micromorphological features of Mycale spicules, may shed...
Tipo: Article / Letter to the editor
Palavras-chave: Poecilosclerida; Mycalidae; Mycale; Taxonomy; Micromorphology; Phylogeny; Microscleres; Scanning electron microscopy.
Ano: 1998
URL: http://www.repository.naturalis.nl/record/534412

Reestablishment of Pycreus section Tuberculati (Cyperaceae)
Provedor de dados: 16
Autores: Reynders, M.; Goetghebeur, P.
Pycreus sect. Tuberculati was created by Chermezon to contain a single species with derived nutlets: P. divulsus, a Malagasy endemic. Kükenthal transferred this species to his new section Muricati. However, a detailed study of the nutlet epidermis shows P. divulsus is not closely related to the other species Kükenthal placed in the latter section. In addition P. divulsus subsp. africanus is upgraded to the species level based on its larger, smooth nutlets and African continental range.
Tipo: Article / Letter to the editor
Palavras-chave: Africa; Cyperaceae; Pycreus sect. Tuberculati; Pycreus africanus; Pycreus divulsus; Scanning electron microscopy; Taxonomy.
Ano: 2010
URL: http://www.repository.naturalis.nl/record/525530

Floral development in three species of Impatiens (Balsaminaceae)
Provedor de dados: 16
Autores: Caris, P.L.; Geuten, K.P.; Janssens, S.B.; Smets, E.
The floral morphological and developmental patterns in three species of Impatiens (Balsaminaceae), namely I. columbaria, I. hawkeri, and I. niamniamensis, were studied to contribute to a better understanding of floral evolution in the genus. Strangely enough, the highly diverse floral morphology and ontogeny of this horticulturally important genus have never been studied thoroughly (e.g., using scanning electron microscopic techniques). We discuss the position and the developmental sequence of the different perianth members. We hypothesized that in the course of evolution, the anterolateral sepals become reduced and that a morphocline can be recognized going from species with five sepals, over species with rudimentary sepals that fuse postgenitally with...
Tipo: Article / Letter to the editor
Palavras-chave: Balsaminaceae; Ericales; Floral development; Floral evolution; Floral morphology; Impatiens; Scanning electron microscopy; 42.40.
Ano: 2006
URL: http://www.repository.naturalis.nl/record/422332

Unravelling species boundaries in the Aspergillus viridinutans complex (section Fumigati): opportunistic human and animal pathogens capable of interspecific hybridization
Provedor de dados: 16
Autores: Hubka, V.; Barrs, V.; Dudová, Z.; Sklenář, F.; Kubátová, A.; Matsuzawa, T.; Yaguchi, T.; Horie, Y.; Nováková, A.; Frisvad, J.C.; Talbot, J.J.; Kolařík, M.
Although Aspergillus fumigatus is the major agent of invasive aspergillosis, an increasing number of infections are caused by its cryptic species, especially A. lentulus and the A. viridinutans species complex (AVSC). Their identification is clinically relevant because of antifungal drug resistance and refractory infections. Species boundaries in the AVSC are unresolved since most species have uniform morphology and produce interspecific hybrids in vitro. Clinical and environmental strains from six continents (n = 110) were characterized by DNA sequencing of four to six loci. Biological compatibilities were tested within and between major phylogenetic clades, and ascospore morphology was characterised. Species delimitation methods based on the multispecies...
Tipo: Article / Letter to the editor
Palavras-chave: Aspergillus felis; Aspergillus fumigatus; Invasive aspergillosis; Mating-type genes; Multispecies coalescence model; Neosartorya udagawae; Scanning electron microscopy; Soil fungi.
Ano: 2018
URL: http://www.repository.naturalis.nl/record/652411

Floral Ontogeny in Scirpus, Eriophorum and Dulichium (Cyperaceae), with Special Reference to the Perianth
Provedor de dados: 16
Autores: Vrijdaghs, A.; Caris, P.; Goetghebeur, P.; Smets, E.
Background and Aims Based on molecular phylogenetic analysis, it has been suggested recently that the Cyperaceae comprises only two subfamilies: the Mapanioideae and the Cyperoideae. In most flowers of the Cyperoideae, the whorl of inner stamens is reduced, resulting in tetracyclic flowers. In the more primitive (scirpoid) genera within the Cyperoideae, the perianth consists of two polysymmetric whorls, whereas the perianth parts in the more derived genera have been subject to modifications and/or reduction. Comparative studies of the many silky hairs of Eriophorum and of the eight bristles of Dulichium have given rise to much discussion about their homology.  Methods The spikelet and floral ontogeny in freshly collected inflorescences was investigated...
Tipo: Article / Letter to the editor
Palavras-chave: Dulichium arundinaceum (L.) Britton; Eriophorum latifolium Hoppe; Floral ontogeny; Perianth; Scirpoid flower; Scirpus sylvaticus L; Scanning electron microscopy.
Ano: 2005
URL: http://www.repository.naturalis.nl/record/407290

Comparative Wood Anatomy of the Primuloid Clade (Ericales s.l.)
Provedor de dados: 16
Autores: Lens, F.; Jansen, S.; Caris, P.; Serlet, L.; Smets, E.
The wood structure of 78 species from 27 genera representing the woody primuloids (Maesaceae, Myrsinaceae, and Theophrastaceae) was investigated using light microscopy (LM) and scanning electron microscopy (SEM). Results indicated that the ray structure, the nature of mineral inclusions, and the occurrence of breakdown areas in rays can be used to separate the three primuloid families from each other. Within Ericales, the presence of exclusively multiseriate rays is synapomorphic for Myrsinaceae and Theophrastaceae, and the occurrence of breakdown areas in rays is synapomorphic for Myrsinaceae. Within Myrsinaceae, the wood structure of the mangrove genus Aegiceras differs because it has short vessel elements that are storied, non-septate fibers, a...
Tipo: Article / Letter to the editor
Palavras-chave: Primuloids; Ericales s.l.; Maesaceae; Myrsinaceae; Theophrastaceae; Wood anatomy; Comparison; Light microscopy; Scanning electron microscopy.
Ano: 2005
URL: http://www.repository.naturalis.nl/record/407307

Floral Ontogeny in Ficinia and Isolepis (Cyperaceae), with Focus on the Nature and Origin of the Gynophore
Provedor de dados: 16
Autores: Vrijdaghs, H.; Goetghebeur, P.; Muasya, A.M.; Caris, P.; Smets, E.
! Background and Aims The generic delimitations of Ficinia and Isolepis, sister genera in the Cypereae, are blurred. Typical Ficinia flowers have a lobed gynophore, which envelops the base of the nutlet, whereas in Isolepis the character is considered to be absent. Some former species of Isolepis, lacking the gynophore, were recently included in Ficinia. The floral ontogeny of representative taxa in Ficinia and Isolepis were investigated with the aim of evaluating the origin and nature of the gynophore in the Cypereae. ! Methods The spikelet and floral ontogeny in inflorescences collected in the field was investigated using scanning electron microscopy (SEM) and light microscopy (LM). ! Key Results SEM images of Isolepis setacea and I. antarctica, Ficinia...
Tipo: Article / Letter to the editor
Palavras-chave: Ficinia; Floral ontogeny; Gynophore; Isolepis; Scanning electron microscopy.
Ano: 2005
URL: http://www.repository.naturalis.nl/record/407286

Synaphridae of Madagascar (Araneae: Araneoidea): A New Family Record for the Afrotropical Region
Provedor de dados: 16
Autores: Miller, J.A.
The spider family Synaphridae is reported for the first time from Madagascar. Three new species are described. Africepheia gen. nov. is established for A. madagascariensis sp. nov.; two species of Synaphris Simon, 1894 are described: S. schlingeri sp. nov. and S. toliara sp. nov. Diagnostic characteristics for the family and putative synapomorphies are discussed, elucidated by scanning electron microscope images representing both Malagasy genera. This research highlights the connection between large scale collecting efforts in biodiversity hotspots like Madagascar and advances in systematics.
Tipo: Article / Letter to the editor
Palavras-chave: Taxonomy; Synapomorphy; Scanning electron microscopy; Africepheia; Cepheia; Synaphris; 42.74.
Ano: 2007
URL: http://www.repository.naturalis.nl/record/306631

Morphological regional differences of epithelial cells along the midgut in Diatraea saccharalis Fabricius (Lepidoptera: Crambidae) larvae
Provedor de dados: 82
Autores: Pinheiro,Daniela O.; Quagio-Grassiotto,Irani; Gregório,Elisa A.
The sugarcane borer, Diatraea saccharalis Fabricius, is a pest to sugarcane and many other crops. This work aims to characterize morphological variability in the epithelial cells (columnar, goblet and regenerative) along the midgut of D. saccharalis larvae. Fragments of the midgut (anterior, middle and posterior regions) were fixed and processed by light and scanning electron microscopy. There are both cytochemical and ultrastructural differences in the morphology of the epithelial cells, depending on their localization along the midgut. The apical surface of columnar cells shows an increase in both number and size of the apical protrusions from the anterior to the posterior midgut regions. There is an increase in the amount of PAS-positive (Periodic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphology; Sugarcane borer; Histochemistry; Scanning electron microscopy; Light microscopy.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2008000400009

Stomatal analysis of citrus somatic hybrids obtained by protoplast fusion
Provedor de dados: 86
Autores: Costa,Maria Angélica Pereira de Carvalho; Almeida,Weliton Antonio Bastos de; Mourão Filho,Francisco de Assis Alves; Mendes,Beatriz Madalena Januzzi; Rodriguez,Adriana Pinheiro Martinelli.
The objective of this work was to evaluate leaf epidermis morphological characteristics of three citrus somatic hybrids, compared to their parents. Parental and somatic hybrid young leaves were collected and processed for scanning electron microscope observations. Citrus polyploid hybrids have fewer stomata per area and these are larger compared to their diploid parental parents. No differences in internal arrangement of the stomatal cells were detected between parental plants and somatic hybrids. Additional studies may determine if these differences will influence physiological behavior of the plants in the field.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stoma; Somatic hybridization; Scanning electron microscopy; Polyploidy.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000300015

Intestinal integrity and performance of broiler chickens fed a probiotic, a prebiotic, or an organic acid
Provedor de dados: 89
Autores: Fernandes,BCS; Martins,MRFB; Mendes,AA; Milbradt,EL; Sanfelice,C; Martins,BB; Aguiar,EF; Bresne,C.
This study aimed at evaluating the intestinal integrity, using light and scanning electron microscopy (SEM), and the performance of broiler chickens fed additives alternative to antimicrobials. A total of 1080 male chicks were distributed according to a completely randomized experimental design, with six treatments with six replicates of 30 birds each. The following treatments were evaluated: basal diet (control), basal diet supplemented with an antimicrobial, basal diet supplemented with a probiotic, basal diet supplemented with a prebiotic, basal diet with a symbiotic, and basal diet supplemented with organic acids. Weight gain, feed intake, feed conversion ratio and livability were recorded when broiler chickens were 10, 21, 35, and 42 days old. On day...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scanning electron microscopy; Morphometrics; Intestinal mucosa; Intestinal wall.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000400012

Chemical-Mineralogical Characterization of Magnetic Materials from Magnetic Soils of the Southern Espinhaço Mountain Chain and of the Upper Jequitinhonha Valley, State of Minas Gerais, Brazil
Provedor de dados: 90
Autores: Silva,Alexandre Christófaro; Souza,Solange de; Fabris,José Domingos; Barbosa,Maurício Soares; Barral,Uidemar Morais; Costa,Roberto Vial.
ABSTRACT In the Southern Espinhaço Mountain Chain and in the Upper Jequitinhonha Valley, magnetic soils, in different pedogenetic stages, are found to be forming over intrusions of basic lithology. The essential chemical and mineralogical properties of samples from magnetic soil profiles from those two physiographic environments in the state of Minas Gerais, Brazil, are reported. Three of the pedons (Rhodic Kandiustox – RKox, Rhodic Haplustox – RHox, and Typic Argiustoll - TAoll) were identified as being indeed developed over basic rocks; the fourth pedon (Typic Haplustox - THox) is currently forming on an acidic rock. Particle size and routine chemical analyses were performed on samples from all horizons of the four selected soil profiles. For a deeper...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Iron oxides; X ray diffractometry; Mössbauer spectroscopy; Scanning electron microscopy; Energy dispersive spectroscopy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100305

Ultrastructure of the antennal sensilla of Alabama argillacea (Hübner, 1823) (Lepidoptera: Erebidae)
Provedor de dados: 92
Autores: Santos,Andrezo Adenilton; Wanderley-Teixeira,Valéria; Cruz,Glaucilane dos Santos; Navarro,Daniela Maria do Amaral Ferraz; Alves,Luiz Carlos; Brayner,Fábio André; Teixeira,Álvaro Aguiar Coelho.
Abstract Insects have several types of sensilla, the characterization of which has been fundamental to understanding the mechanisms of sensory perception in different species. This study aimed to describe the ultrastructure of the sensilla present on the antennae of Alabama argillacea (Hübner, 1823) (Lepidoptera: Erebidae), an important pest of cotton (Gossypium hirsutum L.) crops, as well as their possible variation between sexes. To do this, the antennae of males and females of A. argillacea were analyzed using scanning electron microscopy. Sensilla morphometry was assessed using photomicrographs, from which the lengths and basal and apical diameters of sensilla were measured using the ImageJ program. Seven types of sensilla were identified on the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antenna; Cotton leafworm; Morphometry; Scanning electron microscopy.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262019000200141

Survey of the leg exocrine glands in termites(Isoptera)
Provedor de dados: 92
Autores: Soares,Helena Xavier; Costa-Leonardo,Ana Maria.
A survey of the leg exocrine glands in the termite workers of 16 species of the families Kalotermitidae and Termitidae was carried out through scanning electron microscope. Glandular openings were not found in the legs of Anoplotermes sp., Ruptitermes sp. (Apicotermitinae, Termitidae) or Glyptotermes planus (Kalotermitidae), but they are present, spread over the ventral surface of the first, second and third tarsomeres of other Termitidae such as Armitermes euamignathus, Cornitermes cumulans, Nasutitermes coxipoensis, Rhynchotermes nasutissimus, Syntermes nanus, Embiratermes festivellus (Nasutitermitinae), Amitermes beaumonti, Hoplotermes amplus, Microcerotermes sp., Neocapritermes opacus, Orthognathotermes sp., Spinitermes brevicornutus and Termes sp....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exocrine gland; Leg; Morphology; Scanning electron microscopy; Termite.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262002000100001

Morphology, distribution and abundance of antennal sensilla of the oyster mushroom fly, Coboldia fuscipes (Meigen) (Diptera: Scatopsidae)
Provedor de dados: 92
Autores: Zhang,Zhilin; Li,Xinlian; Chen,Lizhen; Wang,Lihua; Lei,Chaoliang.
ABSTRACT We investigated the distribution, morphology and abundance of antennae sensilla of Coboldia fuscipes (Meigen) using scanning electron microscopy. Antennae of C. fuscipes consisted of scape, pedicel, and flagellum with eight flagellomeres. Antennal scape and pedicel had only one type of sensillum, i.e., sensilla chaetica. Significant differences were found between the number and distribution of these sensilla. Four types of morphologically distinct sensilla on the flagellum were identified, including sensilla chaetica, sensilla trichoidea, sensilla coeloconica, and sensilla basiconica (three subtypes). Significant differences were found in the abundance and distribution of sensilla among the antennal flagella and diverse flagellomeres in both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antennae; Flagellum; Scanning electron microscopy.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262016000100008

Use of scanning electron microscopy for the evaluation of intestinal epithelium integrity
Provedor de dados: 96
Autores: Gomide Junior,Marcio Heber; Sterzo,Elton Vinícius; Macari,Marcos; Boleli,Isabel Cristina.
This work reports the use of scanning electron microscopy to evaluate intestinal epithelium loss in broilers. Intestinal mucosa of male and female chicks submitted to prolonged period post-hatching of water and feed deprivation was evaluated. Two segments of the duodenum, jejunum and ileum were taken per bird, and routinely processed by scanning electron microscopy. Six distinct degrees of epithelium loss were determined: degree 0, normal villi without apparent extrusion; degree 1, villi presenting small areas of epithelium loss; degree 2, villi without epithelium at the apex; degree 3, villi without epithelium at the apical region; degree 4, villi without epithelium in the upper half; degree 5, lack of epithelium in the whole villus; degree 6, loss of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broilers; Fasting; Intestinal epithelium loss; Scanning electron microscopy.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982004000600017

Origin of microaggregates in soils with ferralic horizons
Provedor de dados: 63
Autores: Cooper,Miguel; Vidal-Torrado,Pablo; Chaplot,Vincent.
Microaggregates that characterize ferralic soils have been hypothesized to have physical, geochemical and/or biological origins. Despite of many studies, the hierarchy between these processes that form microaggregates has seldom been reported. The objective of this work was to study the genesis of microaggregates in a sequence of Ferralic Nitisols developed on Quaternary red clayey sediments and diabase in Piracicaba (SP), Brazil. This issue was tackled by combining optical microscopy, image analysis, scanning electron microscopy and elemental iron quantifications by X-ray fluorescence. Micromorphological investigations showed three different types of microaggregates: (i) oval microaggregates with well sorted quartz grains in their interior; (ii) oval...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitisol; Soil genesis; Micromorphology; Scanning electron microscopy.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000300009

External development of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in the subterranean termite Heterotermes tenuis
Provedor de dados: 63
Autores: Moino Jr.,Alcides; Alves,Sérgio Batista; Lopes,Rogério Biaggioni; Neves,Pedro Manuel Oliveira Janeiro; Pereira,Roberto Manoel; Vieira,Solange Aparecida.
The subterranean termite Heterotermes tenuis is one of the main pests of sugarcane and eucalyptus in Brazil, and the use of entomopathogenic fungi, alone or associated to chemicals, is an efficient and environmentally favorable method for its control. Studies related to the fungal development on these insects are important due to the effect of insect behavior on entomopathogens. The objective of this work was to describe the external development of Beauveria bassiana and Metarhizium anisopliae on H. tenuis using Scanning Electron Microscopy (SEM), determining the duration of the different phases of fungal infection. Two fixation techniques for preparing SEM samples were also evaluated. Worker specimens of H. tenuis were inoculated with a 1 x 10(9) conidia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insecta; Isoptera; Microbial control; Scanning electron microscopy.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000200010

Organogenesis from internodal segments of yellow passion fruit
Provedor de dados: 63
Autores: Biasi,Luiz Antonio; Falco,Maria Cristina; Rodriguez,Adriana P. M.; Mendes,Beatriz M. Januzzi.
Organogenesis induction from internodal segments of yellow passionfruit (Passiflora edulis f. flavicarpa) was obtained in a medium supplemented with 1.0-4.0 mg L-1 benzylaminopurine (BAP). Callus proliferation and adventitious shoot development were analyzed by light and scanning electron microscopy. Callus proliferation and adventitious shoots were observed simultaneously and continuously, with asynchronous development of shoots in the explants. Proliferation of callus was observed in the explant cut ends in all treatments, including the control. The results show that concentrations of 1.0 or 2.0 mg L-1 BAP gave better rate of organogenesis. Higher concentrations were efficient for the induction of adventitious buds and, were, however, detrimental for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passiflora edulis f. flavicarpa; Adventitious shoots; Light microscopy; Scanning electron microscopy; Benzylaminopurine.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000400010

Citrus sinensis leaf petiole and blade colonization by Xylella fastidiosa: details of xylem vessel occlusion
Provedor de dados: 63
Autores: Alves,Eduardo; Leite,Breno; Pascholati,Sérgio Florentino; Ishida,Maria Lúcia; Andersen,Peter Craig.
Citrus variegated chlorosis (CVC), caused by Xylella fastidiosa, is an important disease of citrus in Brazil. X. fastidiosa is restricted to xylem vessels of plants and knowledge regarding xylem colonization is still limited. Our goal was to verify how this bacterium colonizes and spreads within xylem vessels of sweet orange Citrus sinensis cv. Pêra. Petioles and pieces of leaf blades from naturally infected plant exhibiting characteristic symptoms were prepared for light microscopy (LM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and immunogold labeling (IGL). Petioles from healthy plants were used as control. IGL results, using an antibody against wall hemicelluloses, revealed that the pit membrane of vessels was altered....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ultrastructure; Scanning electron microscopy; Transmission electron microscopy; Immunolabel.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000200011

Notes on the last instar larva and pupa of Hemiargus hanno (Stoll) (Lepidoptera, Lycaenidae, Polyommatinae)
Provedor de dados: 104
Autores: Duarte,Marcelo; Almeida,Gisele Luziane de; Casagrande,Mirna Martins; Mielke,Olaf H.H.
Larvae of Hemiargus hanno (Stoll, 1790) (Lepidoptera, Lycaenidae, Polyommatinae) are for the first time reported to be attended under field conditions by ants of the formicine genus Brachymyrmex Mayr, 1868 (Myrmelachistini). Structural features of the last instar larva and pupa of H. hanno are described and illustrated with the aid of scanning electron microscopy (SEM).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lycaenidae; Polyommatinae; Hemiargus; Brachymyrmex; Immature stages; Scanning electron microscopy; Morphology; Myrmecophily.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000400008

Morphology and development of leaf papillae in the Pilotrichaceae
Provedor de dados: 39
Autores: Duarte-Silva,Ana Gabriela; Carvalho-Silva,Micheline; Câmara,Paulo Eduardo Aguiar Saraiva.
Sporophyte morphology has been traditionally used in taxonomic studies of pleurocarpous mosses. However, the gametophyte morphology of the Pilotrichaceae still holds interesting and under-investigated characters. Here, we conducted an in-depth investigation of the morphology and development of Pilotrichaceae leaf papillae and assessed their usefulness in inferring the evolutionary history of the family. Specimens were observed under scanning electron microscopy, and we identified seven morphological types of papillae, as well as two different pathways of development. Our results suggest that papilla morphology and development are phylogenetically informative in the Pilotrichaceae.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gametophyte; Hookeriales; Pleurocarpous moss; Outgrowths; Scanning electron microscopy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000400013

Scanning electron microscopy of the leaf epidermis of Merostachys Spreng. (Poaceae: Bambusoideae)
Provedor de dados: 39
Autores: Gomes,Doria Maria Saiter; Neves,Léa de Jesus.
This study presents data on leaf micromorphology of 13 species of Merostachys Spreng. (Poaceae: Bambusoideae), a neotropical genus with numerous endemic species. Greatest species richness is found in Central and Southern Brazil, with 41 of the 46 species described. Species were collected in the field at the localities: Itatiaia National Park and Tijuca National Park, Rio de Janeiro State; Fontes do Ipiranga State Park, São Paulo state; Santa Teresa, Espírito Santo State and Rio Doce State Park, Minas Gerais State. Dried plant material came from the herbarium at the São Paulo Botany Institute. The presence of prickles, silica bodies and macro hairs may be especially useful in delimiting species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bamboo; Leaf; Merostachys; Poaceae; Scanning electron microscopy.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062009000200023

Comparative leaf anatomy of some species of Abies and Picea (Pinaceae)
Provedor de dados: 39
Autores: Ghimire,Balkrishna; Lee,Chunghee; Yang,Jongcheol; Heo,Kweon.
ABSTRACTA number of conifer species are still lacking anatomical data, which is significant because morphological and anatomical data are essential for systematic study. Leaf anatomy was studied in selected species of Abiesand Piceausing light and scanning electron microscopy. Both genera were found to have typical coniferous and highly xerophytic leaves with sunken stomata and an epidermis covered by a thick cuticle. In the genus Abies, species can be differentiated by the nature of the lignified hypodermis and the number and position of resin ducts. Abies firma and A. holophylla have a continuous hypodermis whereas in A. koreana and A. nephrolepis the hypodermis is discontinuous and represented by isolated cells or groups of four or five cells. On the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abies; Leaf anatomy; Picea; Scanning electron microscopy; Taxonomy.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000300346

Pollen morphology and its taxonomic significance in the genus Bomarea Mirb. (Alstroemeriaceae) - II. Subgenus Bomarea
Provedor de dados: 39
Autores: Sarwar,Abul Khayer Mohammad Golam; Hoshino,Yoichiro; Araki,Hajime.
Pollen morphology of 52 species (out of c. 79) of the Bomarea subgenus Bomarea was examined using light microscopy and scanning electron microscopy (SEM), or using SEM alone. The studied species of Bomarea were stenopalynous, characterized by large, oblate, monosulcate monads with reticulate exine sculpture in most species. Wide variation was observed in quantitative palynological features. The studied taxa were divided into four major groups based on exine ornamentation observed under SEM: microreticulate, reticulate, coarsely rugulate, and psilate-perforate. The reticulate exine sculpture may be a plesiomorphic character state for the genus Bomarea, and the coarsely rugulate and finely rugulate-perforate or psilate-perforate exine sculptures may have...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agglomerative hierarchical clustering; Bomarea; Exine sculpture; Infrageneric classification; Scanning electron microscopy.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000400586

Pollen morphology of Centaurea L. (Asteraceae) in Iran
Provedor de dados: 39
Autores: Shabestari,Elaheh Sadat Bagheri; Attar,Farideh; Riahi,Hosein; Sheidai,Masoud.
The genus Centaurea L. is one of the largest genera in the family Asteraceae. The number of taxa included in this genus ranges from 500 to 600 worldwide. Iran is one of the major centers of diversity for this genus. Pollen morphology has proved useful in the systematics of Asteraceae, as well as in that of some of its genera and species. The pollen morphology of 19 taxa of the genus Centaurea was investigated in detail by light and scanning electron microscopy. Examination under light microscopy showed pollen grains to be tricolporate, isopolar, radially symmetrical, oblate spheroidal, prolate spheroidal, subprolate, tectum perforate and scabrate. Based on the exine ornamentation observed under scanning electron microscopy, we identified two types of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Centaurea; Palynology; Exine ornamentation; Light microscopy; Scanning electron microscopy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000400004

Pollen morphology and its taxonomic significance in the genus Bomarea Mirb. (Alstroemeriaceae) - I. Subgenera Baccata, Sphaerine, and Wichuraea
Provedor de dados: 39
Autores: Sarwar,Abul Khayer Mohammad Golam; Hoshino,Yoichiro; Araki,Hajime.
ABSTRACTPollen morphology of 24 of the 33 species of three Bomareasubgenera, Baccata, Sphaerine, andWichuraea, was examined by light microscopy (LM) and scanning electron microscopy (SEM), or SEM alone. The studied species ofBomarea were stenopalynous, characterized by large, monosulcate monads with reticulate exine sculpture in most species. Opercula-like structures were present on the sulcus in B.huanucoand B.involucrosa. Differences in pollen size, exine thickness, and exine sculpture were observed. The studied taxa were divided into four major groups based on exine ornamentation observed under SEM: microreticulate, reticulate, coarsely rugulate, or finely rugulate-perforate. Pollen characters alone did not appear to correlate clearly with the current...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bomarea; Exine sculpture; Infrageneric classification; Opercula-like structures; Scanning electron microscopy.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000300425

Morphology and viability of pollen grains from passion fruit species (Passiflora spp.)
Provedor de dados: 39
Autores: Soares,Taliane Leila; Jesus,Onildo Nunes de; Souza,Everton Hilo de; Santos-Serejo,Janay Almeida dos; Oliveira,Eder Jorge de.
The characterization and viability of pollen grains are useful tools to guide crosses in breeding programs. The objective of this study was to describe the morphological patterns and viability of pollen grains from five accessions of Passiflora edulis f. flavicarpa O. Deg. and five accessions of Passiflora setacea DC. Pollen morphology descriptions were made using light microscopy and scanning electron microscopy, whereas the viability analysis was performed by in vitro germination and histochemical analysis (Lugol's solution and 2,3,5-triphenyltetrazolium chloride). Pollen grains assessed for germination were inoculated in culture medium containing Ca(NO3).4H2O (0.03%), Mg(SO4).7H2O (0.02%), KNO3 (0.01%), H3BO3 (0.01%), sucrose (15%) and agar (0.8%)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro germination; Histochemistry; Scanning electron microscopy; Passifloraceae; Pollen tube.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000400018

Ecophysiological, anatomical and ultrastructural characteristics of Vitex polygama Cham. (Verbenaceae) submitted to different concentrations of fluoride
Provedor de dados: 3
Autores: Rossi, Zélia Terezinha Teixeira; Castro, Evaristo Mauro de; Pereira, Giuslan Carvalho; Barbosa, João Paulo Rodrigues Alves Delfino; Carvalho, Graziele Wolff de Almeida; Rossi, Marco Aurélio de Lima Silva; Pereira, Fabrício José.
Fluorides released during the manufacture of aluminum, fertilizers, glass and ceramics are considered to be highly toxic to plants, causing damages in relatively small concentrations. This study aimed to evaluate fluoride (F) accumulation and its effects on Vitex polygama Cham. (Verbenaceae) exposed to simulated rain containing potassium fluoride in concentrations of 5, 10, 15 and 20 mg L-1. Vitex polygama presented no visual symptoms of foliar injury and accumulated a relatively small amount of F-, significant only for the treatments with higher concentrations of the pollutant.  F- promoted an increase in non-photochemical quenching (qN) and in the coefficient due to non-photochemical extinction (NPQ), which indicates higher dissipation of radiant energy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2.03.00.00-0; 2.03.02.03-7; 2.03.03.00-9 chlorophyll a fluorescence; Potassium fluoride; Scanning electron microscopy; Transmission electron microscopy; Biomonitoring studies Botânica; Anatomia Vegetal; Fisiologia Vegetal.
Ano: 2016
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31400

Effect of ultrasonication of Switchgrass on fermentable sugar production and biomass physical structure
Provedor de dados: 111
Autores: Sharma, Rajat; Sharma-Shivappa, Ratna R.; Boyette, Michael D.; Stikeleather, Larry F.
Pretreatment of lignocellulosic biomass for enhancing sugar yields has been studied extensively over the years.  Conventional methods employed to preprocess biomass to make it conducive for enzymatic hydrolysis have been hampered by issues like poor energy efficiency and production of undesirable by-products.  Ultrasonication, which involves the treatment of biomass through ultrasonic sound waves in a liquid medium without additional chemicals, is believed to have potential for biomass pretreatment.  In this study the effects of ultrasonication on switchgrass, a potential feedstock for bioethanol production due to its high cellulosic content, were investigated.  Results of compositional analysis and scanning electron microscopy conducted to visualize...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Panicum virgatum; Amplitude; Pretreatment; Hydrolysis; Scanning electron microscopy.
Ano: 2013
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/2549

Handling sticky Resin by Stingless Bees: Adhesive Properties of Surface Structures
Provedor de dados: 42
Autores: GASTAUER,MARKUS; CAMPOS,LUCIO A.O.; WITTMANN,DIETER.
Many Stingless Bees (Hymenoptera: Meliponini) like Tetragonisca angustula collect resin to defend their nests against intruders like ants or Robber Bees. Small portions of resin are attached to intruders bodies and extremities causing their immobilization. It has been observed that resin is removed easily from the bee's mandible but adheres strongly to the intruder's cuticle. We tested the hypothesis that resin sticks lesser to the mandibles of Stingless Bees than to the surface of intruders due to special surface structures or adhesive properties of these structures. The surface structures of the mandible of T. angustula and the trochanter of Camponotus sericeiventris were studied by scanning electron microscopy. To measure adhesion properties, selected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Measuring adhesion forces; Scanning electron microscopy; Surface properties; Cuticle; Ultrastructure.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000301189

Microscopic aspects of electrosensory system on the partially euryhaline lesser guitarfish
Provedor de dados: 42
Autores: RANGEL,BIANCA S.; WOSNICK,NATASCHA; AMORIM,ALBERTO F.; K. JUNIOR,JOSÉ ROBERTO; RICI,ROSE E.G.
ABSTRACT The electrosensory system on elasmobranchs consists of subcutaneous electroreceptor organs known as ampullae of Lorenzini. The present study investigated the ampullae of Lorenzini morphology of the lesser guitarfish Zapteryx brevirostris, using light microscopy and scanning electron microscopy. The pore number found in the ventral skin surface is much higher than that found in the dorsal portion, characteristic of species that inhabit the euphotic zone. Under light microscopy it was possible to observe that the wall canal consists of a single layer of squamous epithelial cells. The canal features distal expansion, where the ampullae are located with up to six alveoli. The sensory epithelium of ampullae is composed by cubic cells, with oval...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elasmobranch; Ampullae of Lorenzini; Electroreceptors; Light microscopy; Scanning electron microscopy.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100037

Post-seminal development and morphoanatomy of vegetative and reproductive organs in Stevia rebaudiana (Bert.) Bertoni (Asteraceae)
Provedor de dados: 42
Autores: ROSSI,MÔNICA L.; SOUZA,EVERTON H.; GRANER,ERIKA M.; ALMEIDA,MARCÍLIO DE; MARTINELLI,ADRIANA P.
Abstract Stevia rebaudiana belongs to the Asteraceae family with high economic and medicinal potential. This article describes and illustrates morphological and histological aspects of leaves and reproductive organs, and the germination process, to provide detailed information on this species and to contribute to taxonomic, phylogenetic and pharmacobotanical projects. The fruit is a cypsela, small, simple, dry, indehiscent, monospermic, light or dark colored, with aristate pappus, and the seed presents a spatulate axile embryo. Germination is phaneroepigeal with a pivotal root system and many absorbing root hairs. The leaves are simple, elliptical to obovate, with two types of trichomes (glandular and tector), with a short petiole, exhibiting an opposite...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomy; Germination; Light microscopy; Morphology; Scanning electron microscopy.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502167

Comparative pollen morphological analysis in the subgenera Passiflora and Decaloba
Provedor de dados: 42
Autores: SOARES,TALIANE L.; JESUS,ONILDO N.; SOUZA,EVERTON H.; ROSSI,MÔNICA L.; OLIVEIRA,EDER J.
ABSTRACT The genus Passiflora is the most diversified of the Passifloraceae, and its palynology presents wide morphological variability. The objective of the study was to evaluate the pollen morphology of 18 Passiflora species in order to identify informative pollinic characteristics to contribute to the taxonomic classification of the genus. The morphology of the pollen grains and the exine structure were investigated using light microscopy and scanning electron microscopy. Differences in the pollen morphology were found in the studied species, mainly in terms of shape, pollen aperture and ornamentation pattern of the exine. Most of the species belonging to the subgenus Passiflora presented ornamented 6-syncolpate pollen grains with an oblate-spheroidal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passifloraceae; Pollen grains; Scanning electron microscopy; Exine; Morphological characterization.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502381

Effect of morphological heterogeneity of somatic embryos of Melia azedarach on conversion into plants
Provedor de dados: 47
Autores: Vila,Silvia; Gonzalez,Ana; Rey,Hebe; Mroginski,Luis.
Embryogenic cultures were initiated from immature Melia azedarach (Meliaceae) zigotic embryos. Explants were induced on Murashige and Skoog (1962) medium with 4.54 &amp;#956;M thidiazuron or 0.45 &amp;#956;M dichlorophenoxyacetic acid. After 6 weeks of culture on induction medium, somatic embryos were categorized in four morphological classes based on the presence of single or fused embryos and if they remained united or not to the original explant; that were evaluated histologically. The somatic embryos of every category were transferred, in groups or individually, on a 1/4 MS medium. Bipolar embryos, the more typically normal ones, had well defined shoot and root apical meristems and produced single plants; subcultured individually their conversion was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Somatic embryo morphology; Conversion; Histology; Scanning electron microscopy.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000100002

Potassium silicate and calcium silicate on the resistance of soybean to Phakopsora pachyrhizi infection
Provedor de dados: 53
Autores: Cruz,Maria Fernanda; Rodrigues,Fabrício Ávila; Diniz,Ana Paula Cardoso; Moreira,Maurilio Alves; Barros,Everaldo Gonçalves.
The control of Asian Soybean Rust (ASR), caused by Phakopsora pachyrhizi, has been difficult due to the aggressiveness of the pathogen and the lack of resistant cultivars. The objective of this study was to evaluate the effects of spray of potassium silicate (PS) and soil amendment with calcium silicate (CS) on soybean resistance to ASR. The PS solution was sprayed to leaves 24 hours prior to fungal inoculation while CS was amended to the soil at thirty-five days before sowing. The infection process of P. pachyrhizi was investigated by scanning electron microscopy. The uredia on leaves of plants sprayed with PS were smaller and more compact than those observed on the leaves of plants grown in soil amended with CS or in soil non-amended with CS (control...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Biotrophic fungi; Scanning electron microscopy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052013000400008

Early somatic embryogenesis in Heliconia chartacea Lane ex Barreiros cv. Sexy Pink ovary section explants
Provedor de dados: 52
Autores: Ulisses,Cláudia; Camara,Terezinha Rangel; Willadino,Lilia; Albuquerque,Cynthia Cavalcanti de; Brito,Júlio Zoé de.
The present work evaluated the development of embryogenic callus from transversal ovary sections. The experiments were carried out under two experimental regimes using combinations of IAA (0; 5.71; 8.56; 11.42; 14.27μM) and 2,4-D (0; 13.57; 18.10; 22.62μM) or combinations of 2,4-D with BA (0; 4.43; 6.65; 8.87; 11.09μM). Assessments were made of anatomical aspects of the callus and for the presence of embryogenic structures using cytochemical and histological analyses and stereomicroscopic and scanning electronic microscopic observations. Treatments with 2,4-D and IAA produced friable calluses demonstrating cellular acquisition of morphogenetic competence as well as the formation of pro-embryogenic sectors. The expression of embryogenic program could be...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Callogenesis; Growth regulator; Histology; Scanning electron microscopy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100002

Bubaline Diaphragm Matrix: Development and Clinical Assessment into Cattle Abdominal Hernia Repair
Provedor de dados: 52
Autores: Vora,Shruti Dineshbhai; Kumar,Vineet; Asodiya,Foram Arvindbhai; Singh,Vivek Kumar; Fefar,Dhaval Tribhovanbhai; Gajera,Harsukh Popatbhai.
Abstract The purpose of the study was to develop a xenogenic bubaline diaphragm matrix (BDM) for abdominal hernia repair. A fresh diaphragm was decellularized using aqueous sodium dodecyl sulfate (SDS) solutions (0.5-4% w/v) over a period. Acellularity was confirmed histologically and characterized by Masson’s trichrome staining, scanning electron microscopy (SEM), DNA quantification, agarose gel electrophoresis, and Fourier-transform infrared spectroscopy. The BDM was used for clinical abdominal hernia repair in six cattle. Clinical, hemato-biochemical and antioxidant parameters were evaluated to assess biocompatibility of xenogenic BDM. Histologically, the diaphragm treated with 2% SDS for 48 h showed complete acellularity and orderly arranged collagen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biocompatibility; Bubaline diaphragm matrix; DNA quantification; Spectroscopy; Scanning electron microscopy.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100309

Production of grape juice powder obtained by freeze-drying after concentration by reverse osmosis
Provedor de dados: 52
Autores: Gurak,Poliana Deyse; Cabral,Lourdes Maria Correa; Rocha-Leão,Maria Helena.
This study aimed to evaluate the freeze-drying process for obtaining grape juice powder by reverse osmosis using 50% grape juice pre-concentrated (28.5 °Brix) and 50% hydrocolloids (37.5% maltodextrin and 12.5% arabic gum). The morphology of the glassy food showed the absence of crystalline structure, which was the amorphous wall that protected the contents of the powder. The samples were stored in clear and dark containers at room temperature, evaluated for their physical (X-ray diffraction) for 65 days and chemical (polyphenol content) stability for 120 days. During the storage time in plastic vessels, samples remained physically stable (amorphous) and the phenolic concentration was constant, indicating the potentiality of this technique to obtain a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenolic compounds; Maltodextrin; Arabic gum; X-ray diffraction; Scanning electron microscopy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600017

Structural analysis of Castanea sativa Mill. leaves from different regions in the tree top
Provedor de dados: 52
Autores: Pinto,Teresa Maria; Anjos,Maria do Rosário; Martins,Nuno Miguel; Gomes-Laranjo,José; Ferreira-Cardoso,Jorge; Peixoto,Francisco.
The aim of this work was to perform the histological characterization of the C. sativa leaves of three Portuguese cultivars to establish comparison among the leaves of the different quadrants in accord and with the cardinal points of the tree top and among different cultivars of this species, using light microscopy (OM) and scanning electron microscopy (SEM). Measurements were also carried out for the leaf tissue thickness, stomatal density, leaf area in the four tree top quadrants. The leaves turned to the North had lesser thickness of mesophyll mainly due to lower amount of palisade parenchyma. The stomatal density was significantly lower in these leaves, unlike the leaf area that has the highest expression.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C. sativa; Leaf anatomy; Palisade parenchyma; Spongy parenchyma; Stomatal density; Scanning electron microscopy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100016

In vitro inhibition of adhesion of Escherichia coli strains by Xylitol
Provedor de dados: 52
Autores: Silva,Annelisa Farah da; Suzuki,Érika Yoko; Ferreira,Aline Siqueira; Oliveira,Murilo Gomes; Silva,Sílvio Silvério da; Raposo,Nádia Rezende Barbosa.
The present study aimed to evaluate xylitol's antimicrobial and anti-adherence activities on Escherichia coli (ATCC 8739) and on another clinical strain enteropathogenic E. coli (EPEC). In vitro minimum inhibitory concentration (MIC) test and adhesion assays were performed using 0.5, 2.5 and 5.0% xylitol. It was found that xylitol did not have antimicrobial properties on these strains. The scanning electron microscopy (SEM) demonstrated that the slides treated with xylitol had a significant reduction in the number of bacilli and the inhibition of microbial adhesion was probably the xylitol's mechanism of action. Xylitol could be a possible alternative on the control of E. coli infections.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial adhesion; Escherichia coli; Scanning electron microscopy; Xylitol.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200003

Studies of the small bowel surface by scanning electron microscopy in infants with persistent diarrhea
Provedor de dados: 56
Autores: Fagundes-Neto,U.; De Martini-Costa,S.; Pedroso,M.Z.; Scaletsky,I.C.A.
We describe the ultrastructural abnormalities of the small bowel surface in 16 infants with persistent diarrhea. The age range of the patients was 2 to 10 months, mean 4.8 months. All patients had diarrhea lasting 14 or more days. Bacterial overgrowth of the colonic microflora in the jejunal secretion, at concentrations above 10(4) colonies/ml, was present in 11 (68.7%) patients. The stool culture was positive for an enteropathogenic agent in 8 (50.0%) patients: for EPEC O111 in 2, EPEC O119 in 1, EAEC in 1, and Shigella flexneri in 1; mixed infections due to EPEC O111 and EAEC in 1 patient, EPEC O119 and EAEC in 1 and EPEC O55, EPEC O111, EAEC and Shigella sonnei in 1. Morphological abnormalities in the small bowel mucosa were observed in all 16 patients,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Persistent diarrhea; Small bowel; Scanning electron microscopy; Bacterial proliferation; Infants.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200006

High-impact exercise in rats prior to and during suspension can prevent bone loss
Provedor de dados: 56
Autores: Yanagihara,G.R.; Paiva,A.G.; Gasparini,G.A.; Macedo,A.P.; Frighetto,P.D.; Volpon,J.B.; Shimano,A.C.
High-impact exercise has been considered an important method for treating bone loss in osteopenic experimental models. In this study, we investigated the effects of osteopenia caused by inactivity in femora and tibiae of rats subjected to jump training using the rat tail suspension model. Eight-week-old female Wistar rats were divided into five groups (n=10 each group): jump training for 2 weeks before suspension and training during 3 weeks of suspension; jump training for 2 weeks before suspension; jump training only during suspension; suspension without any training; and a control group. The exercise protocol consisted of 20 jumps/day, 5 days/week, with a jump height of 40 cm. The bone mineral density of the femora and tibiae was measured by double...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone; Mechanical properties; Bone mineral density; Scanning electron microscopy; Jump; Rats.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300605

In vitro biofilm forming potential of Streptococcus suis isolated from human and swine in China
Provedor de dados: 58
Autores: Dawei,Guo; Liping,Wang; Chengping,Lu.
Streptococcus suis is a swine pathogen and also a zoonotic agent. The formation of bioﬁlms allows S. suis to become persistent colonizers and resist clearance by the host immune system and antibiotics. In this study, biofilm forming potentials of various S. suis strains were characterized by confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM) and tissue culture plates stained with crystal violet. In addition, the effects of five antimicrobial agents on biofilm formation were assayed in this study. S. suis produced biofilms on smooth and rough surface. The nutritional contents including glucose and NaCl in the growth medium modulated biofilm formation. There was a significant difference in their biofilm-forming ability among all 46...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilm; Streptococcus suis; Scanning electron microscopy; Confocal laser scanning microscopy; Microplate assay; Antimicrobial agents.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300021

Formation of biofilms by Staphylococcus aureus on stainless steel and glass surfaces and its resistance to some selected chemical sanitizers
Provedor de dados: 58
Autores: Marques,Simone Cristina; Rezende,Jaíne das Graças Oliveira Silva; Alves,Lizandra Aparecida de Freitas; Silva,Belami Cássia; Alves,Eduardo; Abreu,Luiz Ronaldo de; Piccoli,Roberta Hilsdorf.
The objectives of this work were to verify the capability of Staphylococcus aureus of forming bio-film on stainless steel and glass surfaces; to evaluate the efficiency of sodium dichloroisocyanurate, hydrogen peroxide and peracetic acid in inactivating Staphylococcus aureus cells adhered onto these surfaces; and to visualize biofilm development by scanning electron microscopy before and after sanitizer treatment. The surfaces studied consisted of 10x20mm chips immersed in Petri dishes containing BHI broth inoculated with S. aureus ATCC 25923. Biofilm formation was observed after 15-day incubation, when the cells were removed using the swab technique, followed by Baird Parker agar plating. Also, the efficiency of the chemical sanitizers on the chip...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biofilm; Stainless steel; Glass; Staphylococcus aureus; Sanitizers; Scanning electron microscopy.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300029

Structure of xanthan gum and cell ultrastructure at different times of alkali stress
Provedor de dados: 58
Autores: Luvielmo,Márcia de Mello; Borges,Caroline Dellinghausen; Toyama,Daniela de Oliveira; Vendruscolo,Claire Tondo; Scamparini,Adilma Regina Pippa.
Abstract The effect of alkali stress on the yield, viscosity, gum structure, and cell ultrastructure of xanthan gum was evaluated at the end of fermentation process of xanthan production by Xanthomonas campestris pv. manihotis 280-95. Although greater xanthan production was observed after a 24 h-alkali stress process, a lower viscosity was observed when compared to the alkali stress-free gum, regardless of the alkali stress time. However, this outcome is not conclusive as further studies on gum purification are required to remove excess sodium, verify the efficiency loss and the consequent increase in the polymer viscosity. Alkali stress altered the structure of xanthan gum from a polygon-like shape to a star-like form. At the end of the fermentation,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Xanthomonas campestris; Xanthan production; Alkali stress; Transmission microscopy; Scanning electron microscopy.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100102

Effect of fungicide on Fusarium verticillioidesmycelial morphology and fumonisin B1 production
Provedor de dados: 58
Autores: Miguel,Tatiana de Á.; Bordini,Jaqueline G.; Saito,Gervásio H.; Andrade,Célia G.T. de J.; Ono,Mario A.; Hirooka,Elisa Y.; Vizoni,Édio; Ono,Elisabete Y.S.
The effect of fludioxonil + metalaxyl-M on the mycelial morphology, sporulation and fumonisin B1 production by Fusarium verticillioides 103 F was evaluated. Scanning electron microscopy analysis showed that the fungicide caused inhibition of hyphal growth and defects on hyphae morphology such as cell wall disruption, withered hyphae, and excessive septation. In addition, extracellular material around the hyphae was rarely observed in the presence of fludioxonil + metalaxyl-M. While promoting the reduction of mycelial growth, the fungicide increased sporulation of F. verticillioides compared to the control, and the highest production occurred on the 14th day in the treatments and on the 10th day in the control cultures. Fumonisin B1production in the culture...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxigenic fungi; Mycotoxin; Scanning electron microscopy; Electron micrographs; Extracellular material.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100293

Architecture and relevance of several strongly adhered biofilms over a polyester imide (PEI) surface
Provedor de dados: 58
Autores: Soncini-Júnior,Giancarlo; Franchetti,Sandra Mara Martins; Marconato,José Carlos.
A microscopical study was undertaken to analyze the eventual fungi adhesion over a polyester imide surface in enameled copper wires. Scanning electron microscopy allowed to observe in these adhered biofilms, a high amount of pigments, hyphae and its enzymatic arsenal probably acting in the surface of the polymer. Due to the highly aromatic nature of this polyester and the residual phenol derivates still present in the reticulated polymer, an antifungal activity could be expected, but no significant changes were noted in fungal growing and in the adhesion process. Additionally, the wires showed completely failure in all insulation properties. The studies aimed to understand and evaluate the great fungi potential that could be explored in biodeterioration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fungi; Biofilms; Hyphae; Pigments; Adhesion; Scanning electron microscopy; Polymeric surface; Polyester-imide; Enameled copper wires.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500036

Leaf Surface Scanning Electron Microscopy of 16 Mulberry Genotypes (Morus spp.) with Respect to their Feeding Value in Silkworm (Bombyx mori L.) Rearing
Provedor de dados: 117
Autores: Singhal,B.K; Baqual,M.F; Khan,M.A; Bindroo,B.B; Dhar,A.
Mulberry (Morus spp.) is the only silkworm (Bombyx mori L.) food plant. In Indian sub tropics, S-146 is the only popular and ruling mulberry genotype for silkworm rearing. As a result, mulberry leaf availability is always the limiting factor, and therefore, sub tropics are contributing less than 1% of the country’s total silk production compared with more than 60% under tropical conditions. Besides climatic conditions, this is due to a very limited number of mulberry genotypes available in this region for silkworm rearing. However, in the mean time, 15 mulberry genotypes viz. &amp;#8216;Tr-10&amp;#8217;,&amp;#8216;Chinese White&amp;#8217;,&amp;#8216;K-2&amp;#8217;,&amp;#8216;Sujanpur...
Tipo: Journal article
Palavras-chave: Scanning electron microscopy; Stomata; Trichomes; Idioblasts; Leaf yield; Sericulture.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000200001

Essential oils and whole milk in the control of soybean powdery mildew
Provedor de dados: 65
Autores: Perina,Fabiano José; Alves,Eduardo; Pereira,Ricardo Borges; Lucas,Gilvaine Ciavareli; Labory,Claudia Regina Gontijo; Castro,Hilário Antonio de.
This research aimed to evaluate the potential of essential oils (EOs) and cow's whole milk (CWM) in order to control soybean powdery mildew and to estimate the most effective concentrations of these natural products in reducing the disease severity on soybean plants. Three experiments were carried out: The first experiment evaluated and selected the most effective treatments to reduce the severity of soybean powdery mildew under greenhouse conditions; the second experiment evaluated the effect of CWM and EOs of citronella, lemongrass, eucalyptus, cinnamon and tea tree on the pathogen through the ultrastructure analysis of soybean leaflets infected by Erysiphe diffusa using the scanning electron microscope (SEM) and light microscope (LM) technology. In the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Erysiphe diffusa; Glycine max; Essential oil as biocontrol; Scanning electron microscopy; Effective concentrations.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013001100003

Ultrastructural morphology and morphometry of the normal corneal endothelium of adult crossbred pig
Provedor de dados: 65
Autores: Tamayo-Arango,Lynda Jhailú; Baraldi-Artoni,Silvana Martinez; Laus,José Luiz; Vicenti,Felipe Antonio Mendes; Pigatto,João Antonio; Abib,Fernando Cesar.
Corneal endothelium constitutes a monolayer of polygonal cells. The integrity and health of this layer are essential for the maintenance of normal corneal transparency. This study reported by the first time in a detailed way the ultrastructural morphology and morphometry of the corneal endothelium in normal adult crossbred pigs by using scanning electron microscopy (SEM). A regular pattern of polygonal cells, with predominantly hexagonal cells and clear cell borders, was observed. An oval nucleus that bulges in the centre of the cell, cilia (2-4) in a few peripheral cells, openings of the pinocytotic vesicles, microvilli, borders bars and interdigitated cell borders were observed. The mean endothelial cell area was significantly higher (P&lt;0.05) in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cornea endothelium; Pig; Scanning electron microscopy.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100018

The use of scaning electron microscopy in postvaccinal evaluation of tracheal epithelium of Coturnix coturnix japonica
Provedor de dados: 65
Autores: Santin,Elizabeth; Lima,Fabiana S.; Paulillo,Antônio C.; Nakaghi,Laura S. O.; Maiorka,Alex.
This study aimed at evaluating the use of scanning electron microscopy in the study of the post-vaccinal respiratory reaction of the tracheal epithelium of quails (Coturnix coturnix japonica) immunized against Newcastle disease. A number of 36 quails were distributed into four groups: T1 &lt;FONT FACE=Symbol&gt;¾&lt;/FONT&gt; control birds (non-vaccinated); T2 &lt;FONT FACE=Symbol&gt;¾&lt;/FONT&gt; birds vaccinated with Ulster 2C strain; t3 &lt;FONT FACE=Symbol&gt;¾&lt;/FONT&gt; birds vaccinated with B1 strain; t4 &lt;FONT FACE=Symbol&gt;¾&lt;/FONT&gt; birds vaccinated with LaSota strain. Regardless the experimental group, birds did not show detectable clinical signs of post-vaccinal respiratory reaction. However, the analysis of tracheal fragments by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coturnix coturnix japonica; Newcastle disease; Scanning electron microscopy; Respiratory post-vaccinal reaction.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000100019

In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential
Provedor de dados: 64
Autores: Rodrigues,Fabíola Rebouças; Almeida,Weliton Antonio Bastos de; Ledo,Carlos Alberto da Silva; Soares,Taliane Leila; Rossi,Mônica Lanzoni; Santana,José Raniere Ferreira de.
ABSTRACT Vernonia condensata Baker has been traditionally used in folk medicine for the treatment of several inflammatory and infectious processes. Overexploitation of this plant species has drastically reduced its population in its natural habitat (Cerrado). Therefore, tissue culture tools, such as somatic embryogenesis, can be used as an alternative method for rapid and large-scale plant regeneration. The objectives of this study were to induce callogenesis in Vernonia condensata from different types of explants and to evaluate the structural aspects of the development of pro-embryogenic masses of this species by means of histological analyses. The formation of calli was induced from leaf explants and internodal segments, which were inoculated in EME...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alumã; In vitro culture of calli; Anatomical analysis; Scanning electron microscopy; Growth regulators.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542020000100201

Morphology and pollen viability of Lolium multiflorum Lam.
Provedor de dados: 64
Autores: Nunes,Renata de Castro; Bustamante,Fernanda de Oliveira; Techio,Vânia Helena; Mittelmann,Andréa.
Study and characterization of pollen grains are essential for different areas, especially taxonomy, genetic improvement, phylogeny, and paleobotany. As yet, there are no reports on pollen morphology of genotypes of naturalized Lolium multiflorum Lam., introduced cultivars or breeding populations, diploid or polyploid. Ten genotypes of annual ryegrass (L. multiflorum) were evaluated for the viability of pollen grains using propionic carmine and Alexander's stains, while morphology was assessed by the acetolysis technique. Measures of polar axis (P), equatorial diameter (E), exine thickness, and analysis of pollen grains were obtained by scanning electron microscopy (SEM). All genotypes showed high rate of pollen viability (&gt; 89%) for both stains. There...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scanning electron microscopy; Ryegrass; Acetolysis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000200006