Consulta: subjectFacets:"Screening system"
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Data/hora: 07/06/2024 11:09:07

A DNA pooling based system to detect Escherichia coli virulence factors in fecal and wastewater samples
Provedor de dados: 58
Autores: Luz María Chacón,J; Lizeth Taylor,C; Carmen Valiente,A; Irene Alvarado,P; Ximena Cortés,B.
The availability of a useful tool for simple and timely detection of the most important virulent varieties of Escherichia coli is indispensable. To this end, bacterial DNA pools which had previously been categorized were obtained from isolated colonies as well as selected in terms of utilized phenotype; the pools were assessed by two PCR Multiplex for the detection of virulent E. coli eaeA, bfpA, stx1, stx2, ipaH, ST, LT, and aatA genes, with the 16S gene used as DNA control. The system was validated with 66 fecal samples and 44 wastewater samples. At least one positive isolate was detected by a virulent gene among the 20 that were screened. The analysis of fecal samples from children younger than 6 years of age detected frequencies of 25% LT positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Virulence genes; Wastewater; Fecal; Screening system; Escherichia coli.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400012