Consulta: subjectFacets:"Septic shock"
Registros recuperados: 10
Data/hora: 06/06/2024 05:27:05

NMR Structure of rALF-Pm3, an Anti-Lipopolysaccharide Factor from Shrimp: Model of the Possible Lipid A-Binding Site
Provedor de dados: 5
Autores: Yang, Yinshan; Boze, Helene; Chemardin, Patrick; Padilla, Andre; Moulin, Guy; Tassanakajon, Anchalee; Pugniere, Martine; Roquet, Francoise; Destoumieux Garzon, Delphine; Gueguen, Yannick; Bachere, Evelyne; Aumelas, Andre.
The anti-lipopolysaccharide factor ALF-Pm3 is a 98-residue protein identified in hemocytes from the black tiger shrimp Penaeus monodon. It was expressed in Pichia pastoris from the constitutive glyceraldehyde-3-phosphate dehydrogenase promoter as a folded and N-15 uniformly labeled rALF-Pm3 protein. Its 3D structure was established by NMR and consists of three alpha-helices packed against a four-stranded beta-sheet. The C-34-C-55 disulfide bond was shown to be essential for the structure stability. By using surface plasmon resonance, we demonstrated that rALF-Pm3 binds to LPS, lipid A and to OM(R)-174, a soluble analogue of lipid A. Biophysical studies of rALF-Pm3/LPS and rALF-Pm3/OM(R)-174 complexes indicated rather high molecular sized aggregates, which...
Tipo: Text
Palavras-chave: Ultracentrifugation; Surface plasmon resonance; Septic shock; Structure; NMR; Lipid A; Lipopolysaccharide; Anti lipopolysaccharide factor.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6320.pdf

Fluid therapy on patients in septic shock: literature review
Provedor de dados: 195
Autores: Cardoso, Natalia Alves; Sobrinho, Celso Braga.
Defined by the Systemic Inflammatory Response Syndrome (SIRS), which is secondary to an infectious agent, sepsis is a common affection in dogs and cats, being related to high morbidity and mortality rates in these species. The pathogenesis is multifactorial and complex. Hemodynamic instability is being identified as a key factor for the high mortality rate, since it causes hypoxia and subsequent multiple organ dysfunction. The treatment consists of goal-oriented therapy and the primary objective is to restore the circulatory balance through the infusion of large amounts of fluid. However, choosing the adequate fluid therapy is still very controversial. Thus, the objective of this review is to describe the pathophysiology of sepsis and to compare the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sepsis; Septic shock; Fluid resuscitation; Colloids and crystalloids sepse; Choque séptico; Fluidoterapia; Coloides e cristaloides.
Ano: 2018
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/37711

Propolis derivatives inhibit the systemic inflammatory response and protect hepatic and neuronal cells in acute septic shock
Provedor de dados: 55
Autores: Korish,Aida Abdelhamid; Arafa,Maha Mohamed.
BACKGROUND: Severe pathogenic infection triggers excessive release of cytokines as part of the massive inflammatory response associated with septic shock. OBJECTIVES: To investigate the protective effect of caffeic acid phenethye ester (CAPE) against lipopolysaccharide (LPS) induced endotoxemia, hepatic and neuronal damage and the associated systemic inflammatory response (SIR). METHODS: Fifty male Wister rats were divided into: control, LPS, and CAPE+LPS groups. Plasma concentrations of various cytokines, including TNF-α, IL-1α, IL-1β, IL-6, IL-4, IL-10, and sICAM-1 were evaluated. In addition, the histopathological changes in the hepatic and neural cells were assessed. RESULTS: The LPS group showed high inflammatory cytokines and sICAM-1 levels...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipopolysaccharides; Septic shock; Systemic inflammatory response syndrome; Cytokines.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400006

Vibrio vulnificus infection in São Paulo, Brazil: case report and literature review
Provedor de dados: 55
Autores: Araujo,Maria Rita Elmor de; Aquino,Caroline; Scaramal,Elton; Ciola,Claudete Sílvia; Schettino,Guilherme; Machado,Marcel Cerqueira Cesar.
Non cholera Vibrio may cause conjunctivitis, wound infection, gastroenteritis and serious sepsis. Transmission to men is through contact with skin, mucosa or wounds exposed to marine water, and consumption of certain barely cooked or raw seafood, more frequently in the summer. This is one of the first cases of severe infection related to Vibrio vulnificus described in Brazil. The patient was an old man, who ingested seafood in Guarujá, a seashore city near São Paulo, 3 days before hospitalization. He was admitted to the emergency room in an ill state with septic shock. On 2 sets of blood culture a highly virulent microorganism was isolated, Vibrio vulnificus, which leads to sepsis and frequently to death in susceptible patients. The objective of this...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Vibrio vulnificus; Septic shock; Risk factors.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200029

Short-lasting systemic and regional benefits of early crystalloid infusion after intravenous inoculation of dogs with live Escherichia coli
Provedor de dados: 56
Autores: Garrido,A.G.; Poli de Figueiredo,L.F.; Cruz Jr.,R.J.; Silva,E.; Rocha e Silva,M.
We investigated the systemic and regional hemodynamic effects of early crystalloid infusion in an experimental model of septic shock induced by intravenous inoculation with live Escherichia coli. Anesthetized dogs received an intravenous infusion of 1.2 x 10(10) cfu/kg live E. coli in 30 min. After 30 min of observation, they were randomized to controls (no fluids; N = 7), or fluid resuscitation with lactated Ringer's solution, 16 ml/kg (N = 7) or 32 ml/kg (N = 7) over 30 min and followed for 120 min. Cardiac index, portal blood flow, mean arterial pressure, systemic and regional oxygen-derived variables, blood lactate, and gastric PCO2 were assessed. Rapid and progressive cardiovascular deterioration with reduction in cardiac output, mean arterial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiac output; Escherichia coli; Gas tonometry; Portal blood flow; Septic shock.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600009

Furosemide is associated with acute kidney injury in critically ill patients
Provedor de dados: 56
Autores: Levi,T.M.; Rocha,M.S.; Almeida,D.N.; Martins,R.T.C.; Silva,M.G.C.; Santana,N.C.P.; Sanjuan,I.T.; Cruz,C.M.S.
Acute kidney injury (AKI) is common in critically ill patients. Diuretics are used without any evidence demonstrating a beneficial effect on renal function. The objective of the present study is to determine the incidence of AKI in an intensive care unit (ICU) and if there is an association between the use of furosemide and the development of AKI. The study involved a hospital cohort in which 344 patients were consecutively enrolled from January 2010 to January 2011. A total of 132 patients (75 females and 57 males, average age 64 years) remained for analysis. Most exclusions were related to ICU discharge in the first 24 h. Laboratory, sociodemographic and clinical data were collected until the development of AKI, medical discharge or patient death. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acute renal failure; Critically ill patients; Furosemide; Septic shock.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900006

Meningoencephalitis caused by a zygomycete fungus (Basidiobolus) associated with septic shock in an immunocompetent patient: 1-year follow-up after treatment
Provedor de dados: 56
Autores: Auxiliadora-Martins,M.; Alkmim-Teixeira,G.C.; Machado-Viana,J.; Nicolini,E.A.; Martins-Filho,O.A.; Bellissimo-Rodrigues,F.; Carlotti Jr.,C.G.; Basile-Filho,A.
Zygomycosis is an infection caused by opportunistic fungi of the Zygomycetes class, specifically those from the Mucorales and Entomophthorales orders. It is an uncommon disease, mainly restricted to immunocompromised patients. We report a case of a 73-year-old male patient with a history of fever (39°C) lasting for 1 day, accompanied by shivering, trembling, and intense asthenia. The patient was admitted to the intensive care unit with complex partial seizures, and submitted to orotracheal intubation and mechanical ventilation under sedation with midazolam. The electroencephalogram showed evidence of non-convulsive status epilepticus. There is no fast specific laboratory test that permits confirmation of invasive fungal disease. Unless the physician...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Zygomycete; Septic shock; Status epilepticus; Meningoencephalitis; ICU setting.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000800013

13CO2 recovery fraction in expired air of septic patients under mechanical ventilation
Provedor de dados: 56
Autores: Martins,M.A.; Coletto,F.A.; Martins-Filho,O.A.; Marchini,J.S.; Basile-Filho,A.
The continuous intravenous administration of isotopic bicarbonate (NaH13CO2) has been used for the determination of the retention of the 13CO2 fraction or the 13CO2 recovered in expired air. This determination is important for the calculation of substrate oxidation. The aim of the present study was to evaluate, in critically ill patients with sepsis under mechanical ventilation, the 13CO2 recovery fraction in expired air after continuous intravenous infusion of NaH13CO2 (3.8 µmol/kg diluted in 0.9% saline in ddH2O). A prospective study was conducted on 10 patients with septic shock between the second and fifth day of sepsis evolution (APACHE II, 25.9 ± 7.4). Initially, baseline CO2 was collected and indirect calorimetry was also performed. A primer of 5 mL...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Recovery fraction; 13CO2; Stable isotope; Septic shock; Intensive care setting.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700003

Systemic and regional hemodynamic effects of enalaprilat infusion in experimental normotensive sepsis
Provedor de dados: 56
Autores: Rahal,L.; Garrido,A.G.; Cruz Jr.,R.J.; Rocha e Silva,M.; Poli-de-Figueiredo,L.F.
Angiotensin-converting enzyme inhibitors have been shown to improve splanchnic perfusion in distinct shock states. We hypothesized that enalaprilat potentiates the benefits of early fluid resuscitation in severe experimental sepsis, particularly in the splanchnic region. Anesthetized and mechanically ventilated mongrel dogs received an intravenous infusion of live Escherichia coli over a period of 30 min. Thereafter, two interventions were performed: fluid infusion (normal saline, 32 mL/kg over 30 min) and enalaprilat infusion (0.02 mg kg-1 min-1 for 60 min) in randomized groups. The following groups were studied: controls (fluid infusion, N = 4), E1 (enalaprilat infusion followed by fluid infusion, N = 5) and E2 (fluid infusion followed by enalaprilat...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiac output; Escherichia coli; Enalaprilat; Portal vein blood flow; Septic shock.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000900008

Blockade of the action of nitric oxide in human septic shock increases systemic vascular resistance and has detrimental effects on pulmonary function after a short infusion of methylene blue
Provedor de dados: 56
Autores: Weingartner,R.; Oliveira,E.; Oliveira,E.S.; Sant'Anna,U.L.; Oliveira,R.P.; Azambuja,L.A.; Friedman,G.
To investigate the role of nitric oxide in human sepsis, ten patients with severe septic shock requiring vasoactive drug therapy and mechanical ventilation were enrolled in a prospective, open, non-randomized clinical trial to study the acute effects of methylene blue, an inhibitor of guanylate cyclase. Hemodynamic and metabolic variables were measured before and 20, 40, 60, and 120 min after the start of a 1-h intravenous infusion of 4 mg/kg of methylene blue. Methylene blue administration caused a progressive increase in mean arterial pressure (60 [55-70] to 70 [65-100] mmHg, median [25-75th percentiles]; P&amp;lt;0.05), systemic vascular resistance index (649 [479-1084] to 1066 [585-1356] dyne s-1 cm-5 m-2; P&amp;lt;0.05) and the left ventricular stroke...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vasodilation; Myocardial depression; Septic shock; Methylene blue; Nitric oxide; Guanylate cyclase.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200009