Consulta: subjectFacets:"Sialic acid"
Registros recuperados: 7
Data/hora: 15/06/2024 16:25:38

Involvement of a Host Erythrocyte Sialic Acid Content in Babesia bovis Infection
Provedor de dados: 19
Autores: TAKABATAKE, Noriyuki; OKAMURA, Masashi; YOKOYAMA, Naoaki; OKUBO, Kazuhiro; IKEHARA, Yuzuru; IGARASHI, Ikuo; 横山, 直明; 五十嵐, 郁男.
Host sialic acid (SA) has recently been suggested to play an important role in erythrocyte (RBC) infection by Babesia spp. The present study attempted to further determine the specific type of SAs important in the RBC invasion. Bovine RBC was found to bear abundant α2-3-linked SA residues but not α2-6-linked SA in nature, confirmed by flow cytometric analysis of the neuraminidase (Nm)-treated RBCs. Lectin-blot analyses revealed the removal of α2-3-linked SAs from the 97-, 33-, and 31-kDa bands by the Nm treatment. Addition of the Nm-treated RBCs into an in vitro culture of B. bovis resulted in a decreased population of the parasitized RBCs. The thin smear samples from the cultures were then observed under a confocal laser scanning microscope after staining...
Palavras-chave: Babesia bovis; Erythrocyte; Invasion; Sialic acid.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4166

Development of a novel method to evaluate sialylation of glycoproteins and analysis of gp96 sialylation in Hela, SW1990 and A549 cell lines
Provedor de dados: 48
Autores: Liang,Yangui; Hua,Qiang; Pan,Pengwei; Yang,Jie; Zhang,Qi.
BACKGROUND: Glycoproteins play a critical role in the cellular activities of eukaryotes. Sialic acid is typically the outermost monosaccharide of glycolipids and glycoproteins, and is necessary for normal development. RESULTS: A strategy based on avidin-biotin affinity was established to enrich sialylated glycoproteins from HeLa cervical carcinoma, SW1990 pancreatic adenocarcinoma, and A549 lung adenocarcinoma cells. Using HPLC-MS/MS, western blot, real-time PCR, and enzyme-linked immunosorbent assay, gp96 was identified in all three cell lines. No significant difference in the protein expression of gp96 was detected at the whole cell level, but the amount of bioti-nylated gp96 in SW1990 cells was 30-40 % lower than that in A549 and HeLa cells, and the...
Tipo: Journal article
Palavras-chave: Click chemistry; Sialic acid; Gp96; Sialyltransferase.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100052

Sperm and Egg Jelly Coat from Sea Urchin Lytechinus variegatus Collected in Rio de Janeiro Contain Distinct Sialic Acid-Rich Polysaccharides
Provedor de dados: 52
Autores: Valle,Gabrielle M.; Cinelli,Leonardo P.; Todeschini,Adriane R.; Brito-Gitirana,Lycia de; Vilela-Silva,Ana-Cristina E.S.; Mourão,Paulo A.S.
This work found the occurrence of a distinct sialic acid-rich polysaccharide in the sperm surface of the sea urchin Lytechinus variegatus, which differed significantly from a similar molecule found in the egg jelly. The sperm polysaccharide extracted by protease digestion was purified using anion exchange chromatography and characterized using agarose gel electrophoresis, gas chromatography/mass spectrometry and NMR spectroscopy. This polysaccharide was highly sulfated and composed almost exclusively of N-acetylneuraminic acid. In contrast, the sialic acid-rich polysaccharide from the egg jelly was composed of N-glycolylneuraminic acid and contains several other hexoses in its structure. This new molecule could help to characterize in further detail the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sea urchin; Sperm; Egg jelly coat; Fertilization; Sialic acid; Sulfated polysaccharide.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400617

Structure-function analysis of two variants of mumps virus hemagglutinin-neuraminidase protein
Provedor de dados: 55
Autores: Santos-López,Gerardo; Scior,Thomas; Borraz-Argüello,María del Tránsito; Vallejo-Ruiz,Verónica; Herrera-Camacho,Irma; Tapia-Ramírez,José; Reyes-Leyva,Julio.
A point mutation from guanine (G) to adenine (A) at nucleotide position 1081 in the hemagglutinin-neuraminidase (HN) gene has been associated with neurovirulence of Urabe AM9 mumps virus vaccine. This mutation corresponds to a glutamic acid (E) to lysine (K) change at position 335 in the HN glycoprotein. We have experimentally demonstrated that two variants of Urabe AM9 strain (HN-A1081 and HN-G1081) differ in neurotropism, sialic acidbinding affinity and neuraminidase activity. In the present study, we performed a structure-function analysis of that amino acid substitution; the structures of HN protein of both Urabe AM9 strain variants were predicted. Based on our analysis, the E/K mutation changes the protein surface properties and to a lesser extent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hemagglutinin-neuraminidasa; Mumps virus; Paramyxovirus; 3D structure prediction; Sialic acid.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000100007

Association between dental-oral health in young adults and salivary glutathione, lipid peroxidation and sialic acid levels and carbonic anhydrase activity
Provedor de dados: 56
Autores: Öztürk,L.K.; Furuncuoğlu,H.; Atala,M.H.; Uluköylü,O.; Akyüz,S.; Yarat,A.
The aim of the present study was to evaluate the relationship between salivary oxidative stress and dental-oral health. Healthy young adults, matched for gender and age, with (N = 21, 10 men, mean age: 20.3 ± 1 years) and without (N = 16, 8 men, mean age: 21.2 ± 1.8 years) caries were included in this study. The World Health Organization (WHO) caries diagnostic criteria were used for determining the decayed, missing, filled teeth (DMFT) index. The oral hygiene and gingival status were assessed using the simplified oral hygiene index and gingival index, respectively. Unstimulated salivary total protein, glutathione (GSH), lipid peroxidation and total sialic acid levels, carbonic anhydrase activity, and salivary buffering capacity were determined by standard...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glutathione; Lipid peroxidation; Sialic acid; Carbonic anhydrase; Dental-oral health.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100003

Sialic acid changes in Dalton's lymphoma-bearing mice after cyclophosphamide and cisplatin treatment
Provedor de dados: 56
Autores: Nicol,B.M.; Prasad,S.B.
Sialic acid changes in Dalton's lymphoma cells and other tissues of 10-12-week-old Swiss albino mice were investigated in relation to tumour growth in vivo and following cyclophosphamide (ip, 200 mg/kg body weight) or cisplatin (ip, 8 mg/kg body weight) treatment. Three to four animals of both sexes were used in each experimental group. The sialic acid level of tumour cells (0.88 µmol/g) increased with tumour progression (1.44-1.59 µmol/g; P&lt;=0.05) in mice. Sialic acid concentration in other tissues (liver, kidney, testes and brain) also increased (~40, 10, 30 and 58%, respectively) in the tumour-bearing hosts as compared with that in the respective tissues of normal mice. In vivo cyclophosphamide or cisplatin treatment resulted in an overall decrease...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sialic acid; Cisplatin; Cyclophosphamide; Dalton's lymphoma.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000500006

Prevalence of Group B Streptococcus serotypes III and V in pregnant women of Rio de Janeiro, Brazil
Provedor de dados: 58
Autores: Soares,Georgia Cristina Tavolaro; Alviano,Daniela Sales; Santos,Gabriela da Silva; Alviano,Celuta Sales; Mattos-Guaraldi,Ana Luiza; Nagao,Prescilla Emy.
GBS serotypes III and V were the most prevalent in pregnant women and exhibited resistance to tetracycline, clindamycin and sulfamethoxazole/trimethoprim. Serotype III showed high sialic acid content and PFGE analysis discerned 33 heterogeneous profiles. Phenotypic and genotypic characterization could be relevant to control GBS infections unaffected by intra-partum chemoprophylaxis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Group B Streptococcus; Antimicrobial susceptibility; Sialic acid; PFGE.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300032