Consulta: subjectFacets:"Snake venom"
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Data/hora: 09/06/2024 08:20:18

Humoral response and neutralization capacity of sheep serum inoculated with natural and Cobalt 60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768)
Provedor de dados: 79
Autores: Netto,D. P.; Chiacchio,S. B.; Bicudo,P. L.; Alfieri,A. A.; Nascimento,N.
The aim of this work was to investigate antigen irradiation on crotalic antivenom and the capacity of sheep as serum producers. Twelve sheep in two groups of six were inoculated with Crotalus durissus terrificus venom. One group was inoculated with natural venom (NV) and the other with Cobalt 60 gamma-irradiated venom (IrV). Three antigen doses were given to the animals at monthly intervals for immunization. The toxic activity of the venom was assessed by LD50 determination in mice. Blood samples were collected weekly analyses of serum neutralization capacity and potency. At the end of the experiment, the animals were challenged with a LD50 for sheep showed no signs of envenoming. These results showed that toxicity of the irradiated venom was 4.4 times...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gamma irradiated crotalic antisera; Snake venom; ELISA; Sheep; Crotalus durissus terrificus; Cobalt60.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000200009

Action of cobra venom on the renal cortical tissues: electron microscopic studies
Provedor de dados: 79
Autores: RAHMY,T. R.
The effect of intramuscular (IM) injection of a sub-lethal dose of Naja haje cobra venom (0.015 mug/gm body weight) on the ultrastructure of renal cortical tissues of rabbits was studied at different time intervals after envenomation. Succinic dehydrogenase (SDH) enzyme activity was also detected in the renal cortical tubules. Three hours after venom injection, slight changes were seen in visceral cells and glomerular endothelia. The cortical tubular epithelia revealed an increase of lysosomal structures, cytoplasmic vacuolization, and nuclear irregularity. Severe ultrastructural changes were recorded 6 hours after envenomation, including hypertrophied parietal cells, blebbed visceral epithelial cells, and foot process disorganization. Dilated glomerular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Nephrotoxicity; Kidney; Ultrastructure.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302001000100007

An evaluation by rat colon anastomosis of the efficacy of fibrin glue derived from snake venom
Provedor de dados: 79
Autores: LEITE,C.V.S.; NARESSE,L.E.; ARANTES,H.L.; LOPES,A.F.; THOMAZINI-SANTOS,I.A.; GIANNINI,M.J.S; MERCADANTE,M.C.; BARRAVIERA,B.; KOBAYASI,S.
The objective of this study was to evaluate the efficacy of fibrin adhesive made up of snake venom and bubaline fibrinogen by rat colon anastomosis. Eighty rats were randomly assigned into 2 experimental groups: GI control (anastomosis with extramucous interrupted suture) and GII (repair suture + fibrin glue). The animals were studied at the following 4 times: T0 – preoperative, T1 – 7th day postoperative, T2 – 14th day postoperative, and T3 – 21 st day postoperative. The macroscopic characteristics of the intestinal segment open and closed anastomosis and the bursting strength of the anastomosed segments were observed at each of the above times. The results showed that the anastomosed segments coapted and there was no difference in the bursting strength...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibrin glue; Experimental healing; Bursting strength; Experimental anastomosis; Snake venom; Colon anastomosis.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302000000200004

ACUTE-PHASE REACTIONS, INCLUDING CYTOKINES, IN PATIENTS BITTEN BY BOTHROPS AND CROTALUS SNAKES IN BRAZIL
Provedor de dados: 79
Autores: BARRAVIERA,B.; LOMONTE,B.; TARKOWSKI,A.; HANSON,L. Å.; MEIRA,D.A.
Thirty-one patients bitten by venomous snakes in Botucatu area (State of São Paulo - Brazil), sixteen by Bothrops spp. and fifteen by Crotalus durissus terrificus, were studied. The group comprised twenty-nine males and two females, ranging from fourteen to sixty-three years of age (mean 33 ± 15). Leukocytosis with neutrophilia and lymphopenia, increase of mucoproteins and C-reactive protein, decrease of total serum protein and albumin, were observed on the first day after the accident. In addition, increased serum levels of the cytokines IL-6 and IL-8, but not of IL-1&lt;FONT FACE="Symbol"&gt;b&lt;/FONT&gt; and TNF-&lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt;, were observed. The alterations were generally more intense in patients bitten by Crotalus durissus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Acute-phase reactions; Cytokines; C-reactive protein; Inflammation.
Ano: 1995
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301995000100003

Surgical adhesives
Provedor de dados: 79
Autores: THOMAZINI-SANTOS,I. A.; BARRAVIERA,S. R. C. S.; MENDES-GIANNINI,M. J. S.; BARRAVIERA,B.
The authors have performed a literature review of surgical adhesives, such as cyanoacrylate, collagen gelatin, and fibrin glue. They have included different types of commercial and non-commercial fibrin sealants and have reported on the different components in these adhesives, such as fibrinogen, cryoprecipitate, bovine thrombin, and thrombin-like fraction of snake venom.
Tipo: Info:eu-repo/semantics/review
Palavras-chave: Fibrin glue; Surgical adhesives; Collagen gelatin; Cyanoacrylates; Fibrinogen; Cryoprecipitate; Bovine thrombin; Thrombin-like fraction; Snake venom.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302001000200002

Antitumor and biochemical effects of Echis coloratus crude venom on Ehrlich ascites carcinoma cells in vivo
Provedor de dados: 79
Autores: MADY,E. A.
The search for snake venom antitumor efficacy has attracted the interest of scientists since the beginning of last century. Snake venom possesses a wide spectrum of biological activities. In this study, we evaluated the effect of Echis coloratus crude venom on the evolution of Ehrlich ascites carcinoma cells (EAC). Normal and EAC-bearing mice were treated with 0.2 mg/kg body weight of crude venom. Crude venom was seen to suppress tumor growth by significantly decreasing EAC cell count and cell viability (p&lt;0.01). There was also a significant increase in survival time of the venom-treated tumor-bearing mice (52.3%, p&lt;0.05) in comparison to the non-treated tumor-bearing counterparts. The study of venom effect and/or tumor inoculation on some important...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Echis coloratus; Ehrlich ascites carcinoma; Tumor growth; Biochemistry.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000200008

Snake venom galactoside-binding lectins: a structural and functional overview
Provedor de dados: 80
Autores: Sartim,Marco A.; Sampaio,Suely V.
AbstractSnake venom galactoside-binding lectins (SVgalLs) comprise a class of toxins capable of recognizing and interacting with terminal galactoside residues of glycans. In the past 35 years, since the first report on the purification of thrombolectin from Bothrops atrox snake venom, several SVgalLs from Viperidae and Elapidae snake families have been described, as has progressive improvement in the investigation of structural/functional aspects of these lectins. Moreover, the advances of techniques applied in protein-carbohydrate recognition have provided important approaches in order to screen for possible biological targets. The present review describes the efforts over the past 35 years to elucidate SVgalLs, highlighting their structure and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; C-type lectin; Galactoside-binding protein; Carbohydrate recognition domain; Glycoconjugates; Inflammatory response; Antitumor activity; Bactericidal activity; Mitogenic activity; Platelet aggregation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100213

Identification and partial purification of an anticoagulant factor from the venom of the Iranian snake Agkistrodon halys
Provedor de dados: 80
Autores: Ghorbanpur,M; Zare Mirakabadi,A; Zokaee,F; Zolfagarrian,H.
An anticoagulant factor was purified from the venom of the Iranian snake Agkistrodon halys by gel filtration on Sephadex G-50 and ion-exchange chromatography on DEAE-Sepharose. In the final stage of purification, the percentage recovery of purified anticoagulant factor was found to be 83%. The purified anticoagulant factor revealed a single protein band in SDS-polyacrylamide electrophoresis under reducing conditions and its molecular weight was about 22 kDa. The purified peptide did not show any effect on casein, BApNA or plasma.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Agkistrodon halys; Anticoagulant factor; Chromatography.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100010

Cytotoxicity of Southeast Asian snake venoms
Provedor de dados: 80
Autores: Jamunaa,A; Vejayan,J; Halijah,I; Sharifah,SH; Ambu,S.
Cytotoxicity of venoms from eleven medically important snakes found in Southeast Asia (Naja kaouthia, Naja siamensis, Naja sumatrana, Ophiophagus hannah, Bungarus candidus, Bungarus fasciatus, Enhydrina schistosa, Calloselasma rhodostoma, Trimeresurus purpureomaculatus and Tropidolaemus sumatranus) was determined, based on the MTS cytotoxicity assay, which determines the survival of viable cells in monolayer MDCK and Vero cell cultures upon exposure to the snake venoms. Snake venom toxicity was expressed as the venom dose that killed 50% of the cells (CTC50) under the assay conditions. Venoms of C. rhodostoma (2.6 µg/mL, 1.4 µg/mL) and O. hannah were the most cytotoxic (3.8 µg/mL, 1.7 µg/mL) whereas N. siamensis venom showed the least cytotoxicity (51.9...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Snake venom; Vipers; Elapids; Kraits.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200004

Unique heterologous fibrin biopolymer with hemostatic, adhesive, sealant, scaffold and drug delivery properties: a systematic review
Provedor de dados: 80
Autores: Buchaim,Daniela Vieira; Cassaro,Claudia Vilalva; Shindo,João Vitor Tadashi Cosin; Coletta,Bruna Botteon Della; Pomini,Karina Torres; Rosso,Marcelie Priscila de Oliveira; Campos,Leila Maria Guissoni; Ferreira Jr,Rui Seabra; Barraviera,Benedito; Buchaim,Rogério Leone.
Abstract Fibrin biopolymers, previously referred as “fibrin glue” or “fibrin sealants”, are natural biomaterials with diverse applications on health. They have hemostatic, adhesive, sealant, scaffold and drug delivery properties and have become widely used in medical and dental procedures. Historically, these biomaterials are produced from human fibrinogen and human or animal thrombin, and the possibility of transmission of infectious diseases by human blood is not ruled out. In the 1990s, to overcome this problem, a new heterologous biomaterial composed of a thrombin-like enzyme purified from Crotalus durissus terrificus venom and a cryoprecipitate rich in fibrinogen extracted from buffaloes Bubalus bubalis blood has been proposed. Therefore, a systematic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibrin sealant; Snake venom; Thrombin-like enzyme; Cryoprecipitate coagulum.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100207

Cytotoxic and inflammatory potential of a phospholipase A2 from Bothrops jararaca snake venom
Provedor de dados: 80
Autores: Cedro,Rafhaella C. A.; Menaldo,Danilo L.; Costa,Tássia R.; Zoccal,Karina F.; Sartim,Marco A.; Santos-Filho,Norival A.; Faccioli,Lúcia H.; Sampaio,Suely V.
Abstract  Background: Snake venom phospholipases A2 (PLA2s) have been reported to induce myotoxic, neurotoxic, hemolytic, edematogenic, cytotoxic and proinflammatory effects. This work aimed at the isolation and functional characterization of a PLA2 isolated from Bothrops jararaca venom, named BJ-PLA2-I.  Methods and Results: For its purification, three consecutive chromatographic steps were used (Sephacryl S-200, Source 15Q and Mono Q 5/50 GL). BJ-PLA2-I showed acidic characteristics, with pI~4.4 and molecular mass of 14. 2 kDa. Sequencing resulted in 60 amino acid residues that showed high similarity to other Bothrops PLA2s, including 100% identity with BJ-PLA2, an Asp49 PLA2 previously isolated from B. jararaca venom. Being an Asp49 PLA2, BJ-PLA2-I...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops jararaca; Phospholipase A2; Inflammation; Cytotoxicity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100324

Heterologous fibrin sealant derived from snake venom: from bench to bedside – an overview
Provedor de dados: 80
Autores: Ferreira Jr,Rui Seabra; Barros,Luciana Curtolo de; Abbade,Luciana Patrícia Fernandes; Barraviera,Silvia Regina Catharino Sartori; Silvares,Maria Regina Cavariani; Pontes,Leticia Gomes de; Santos,Lucilene Delazari dos; Barraviera,Benedito.
Abstract Hemostatic and adhesive agents date back to World War II, when homologous fibrin sealant came onto scene. Considering that infectious diseases can be transmitted via human blood, a new heterologous fibrin sealant was standardized in the 1990s. Its components were a serine protease (a thrombin-like enzyme) extracted from the venom of Crotalus durissus terrificus snakes and a fibrinogen-rich cryoprecipitate extracted from the blood of Bubalus bubalis buffaloes. This new bioproduct has been used as a coagulant, sealant, adhesive and recently as a candidate scaffold for mesenchymal stem cells and bone and cartilage repair. This review discusses the composition of a new heterologous fibrin sealant, and cites published articles related to its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibrin sealant; Snake venom; Cryoprecipitate coagulum; Thrombin-like enzyme; Buffaloes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100204

Functional and biological insights of rCollinein-1, a recombinant serine protease from Crotalus durissus collilineatus
Provedor de dados: 80
Autores: Boldrini-França,Johara; Pinheiro-Junior,Ernesto Lopes; Arantes,Eliane Candiani.
ABSTRACT  Background: The prevalent class of snake venom serine proteases (SVSP) in Viperidae venoms is the thrombin-like enzymes, which, similarly to human thrombin, convert fibrinogen into insoluble fibrin monomers. However, thrombin-like serine proteases differ from thrombin by being unable to activate factor XIII, thus leading to the formation of loose clots and fibrinogen consumption. We report the functional and biological characterization of a recombinant thrombin-like serine protease from Crotalus durissus collilineatus, named rCollinein-1.  Methods: Heterologous expression of rCollinein-1 was performed in Pichia pastoris system according to a previously standardized protocol, with some modifications. rCollinein-1 was purified from the culture...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Serine proteases; Thrombin-like enzymes; Coagulation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100306

The modular nature of bradykinin-potentiating peptides isolated from snake venoms
Provedor de dados: 80
Autores: Sciani,Juliana Mozer; Pimenta,Daniel Carvalho.
Abstract Bradykinin-potentiating peptides (BPPs) are molecules discovered by Sergio Ferreira – who found them in the venom of Bothrops jararaca in the 1960s – that literally potentiate the action of bradykinin in vivo by, allegedly, inhibiting the angiotensin-converting enzymes. After administration, the global physiological effect of BPP is the decrease of the blood pressure. Due to this interesting effect, one of these peptides was used by David Cushman and Miguel Ondetti to develop a hypotensive drug, the widely known captopril, vastly employed on hypertension treatment. From that time on, many studies on BPPs have been conducted, basically describing new peptides and assaying their pharmacological effects, mostly in comparison to captopryl. After...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararaca; Venom; Snake venom; Bradykinin-potentiating peptides; BPP; Modules.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100208

BaltDC: purification, characterization and infrared spectroscopy of an antiplatelet DC protein isolated from Bothrops alternatus snake venom
Provedor de dados: 80
Autores: Matias,Mariana Santos; Sousa,Bruna Barbosa de; Pereira,Déborah Fernanda da Cunha; Dias,Edigar Henrique Vaz; Mamede,Carla Cristine Neves; Queiroz,Mayara Ribeiro de; Silva,Anielle Christine Almeida; Dantas,Noelio Oliveira; Soares,Andreimar Martins; Costa,Júnia de Oliveira; Oliveira,Fábio de.
Abstract  Background: Snake venoms are a complex mixture of proteins, organic and inorganic compounds. Some of these proteins, enzymatic or non-enzymatic ones, are able to interact with platelet receptors, causing hemostatic disorders. The possible therapeutic potential of toxins with antiplatelet properties may arouse interest in the pharmacological areas. The present study aimed to purify and characterize an antiplatelet DC protein from Bothrops alternatus snake venom.  Methods: The protein, called BaltDC (DC protein from B. alternatus snake venom), was purified by a combination of ion-exchange chromatography on DEAE-Sephacel column and gel filtration on Sephadex G-75. The molecular mass was estimated by polyacrylamide gel electrophoresis in the presence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops alternatus; DC protein; Platelet aggregation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100324

Rattlesnake Crotalus molossus nigrescens venom induces oxidative stress on human erythrocytes
Provedor de dados: 80
Autores: Meléndez-Martínez,David; Muñoz,Juan Manuel; Barraza-Garza,Guillermo; Cruz-Peréz,Martha Sandra; Gatica-Colima,Ana; Alvarez-Parrilla,Emilio; Plenge-Tellechea,Luis Fernando.
Abstract  Background  Globally, snake envenomation is a well-known cause of death and morbidity. In many cases of snakebite, myonecrosis, dermonecrosis, hemorrhage and neurotoxicity are present. Some of these symptoms may be provoked by the envenomation itself, but others are secondary effects of the produced oxidative stress that enhances the damage produced by the venom toxins. The only oxidative stress effect known in blood is the change in oxidation number of Fe (from ferrous to ferric) in hemoglobin, generating methemoglobin but not in other macromolecules. Currently, the effects of the overproduction of methemoglobin derived from snake venom are not extensively recorded. Therefore, the present study aims to describe the oxidative stress induced by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Attenuated total reflectance-Fourier transform infrared spectroscopy; Crotalus molossus nigrescens; Venom; Snake venom; Methemoglobin; Oxidative stress; Oxyhemoglobin.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100311

Actiflagelin, a new sperm activator isolated from Walterinnesia aegyptia venom using phenotypic screening
Provedor de dados: 80
Autores: El-Aziz,Tarek Mohamed Abd; Khoury,Sawsan Al; Jaquillard,Lucie; Triquigneaux,Mathilde; Martinez,Guillaume; Bourgoin-Voillard,Sandrine; Sève,Michel; Arnoult,Christophe; Beroud,Rémy; Waard,Michel De.
Abstract  Background Sperm contains a wealth of cell surface receptors and ion channels that are required for most of its basic functions such as motility and acrosome reaction. Conversely, animal venoms are enriched in bioactive compounds that primarily target those ion channels and cell surface receptors. We hypothesized, therefore, that animal venoms should be rich enough in sperm-modulating compounds for a drug discovery program. Our objective was to demonstrate this fact by using a sperm-based phenotypic screening to identify positive modulators from the venom of Walterinnesia aegyptia.  Methods Herein, as proof of concept that venoms contain interesting compounds for sperm physiology, we fractionated Walterinnesia aegyptia snake venom by RP-HPLC and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Walterinnesia aegyptia; Bioactive compounds; Fertility; Sperm motility; Venomics; Tandem mass spectrometry; De novo sequencing; Edman degradation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100301

Molecular cloning of a hyaluronidase fromBothrops pauloensisvenom gland
Provedor de dados: 80
Autores: Castanheira,Letícia Eulalio; Rodrigues,Renata Santos; Boldrini-França,Johara; Fonseca,Fernando PP; Henrique-Silva,Flávio; Homsi-Brandeburgo,Maria I; Rodrigues,Veridiana M.
Background Hyaluronate is one of the major components of extracellular matrix from vertebrates whose breakdown is catalyzed by the enzyme hyaluronidase. These enzymes are widely described in snake venoms, in which they facilitate the spreading of the main toxins in the victim’s body during the envenoming. Snake venoms also present some variants (hyaluronidases-like substances) that are probably originated by alternative splicing, even though their relevance in envenomation is still under investigation. Hyaluronidases-like proteins have not yet been purified from any snake venom, but the cDNA that encodes these toxins was already identified in snake venom glands by transcriptomic analysis. Herein, we report the cloning and in silicoanalysis of the first...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alternative splicing; Hyaluronidase-like; Snake venom.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200330

Antibacterial potential of a basic phospholipase A2(VRV-PL-VIIIa) from Daboia russelii pulchella (Russell's viper) venom
Provedor de dados: 80
Autores: Sudharshan,Shivalingaiah; Dhananjaya,Bhadrapura Lakkappa.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bactericidal; Antibiotics; Drug; Human pathogenic bacteria.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100322

Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature
Provedor de dados: 80
Autores: Tasima,Lídia J.; Serino-Silva,Caroline; Hatakeyama,Daniela M.; Nishiduka,Erika S.; Tashima,Alexandre K.; Sant’Anna,Sávio S.; Grego,Kathleen F.; Morais-Zani,Karen de; Tanaka-Azevedo,Anita M.
Abstract  Background: Crotalus durissus is considered one of the most important species of venomous snakes in Brazil, due to the high mortality of its snakebites. The venom of Crotalus durissus contains four main toxins: crotoxin, convulxin, gyroxin and crotamine. Venoms can vary in their crotamine content, being crotamine-negative or -positive. This heterogeneity is of great importance for producing antivenom, due to their different mechanisms of action. The possibility that antivenom produced by Butantan Institute might have a different immunorecognition capacity between crotamine-negative and crotamine-positive C. durissus venoms instigated us to investigate the differences between these two venom groups.  Methods: The presence of crotamine was analyzed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rattlesnake; Snake venom; Toxins; Venom variation; Antivenom.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100307

In vivo evaluation of homeostatic effects of Echis carinatus snake venom in Iran
Provedor de dados: 80
Autores: Salmanizadeh,Hossein; Babaie,Mahdi; Zolfagharian,Hossein.
Background The venom of the family Viperidae, including the saw-scaled viper, is rich in serine proteinases and metalloproteinases, which affect the nervous system, complementary system, blood coagulation, platelet aggregation and blood pressure. One of the most prominent effects of the snake venom of Echis carinatus (Ec) is its coagulation activity, used for killing prey. Materials and methods Subfractions F1A and F1B were isolated from Ec crude venom by a combination of gel chromatography (Sephadex G-75) and ion exchange chromatography on a DEAE-Sepharose (DE-52). These subfractions were then intravenously (IV) injected into NIH male mice. Blood samples were taken before and after the administration of these subfractions. Times for prothrombin, partial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Procoagulant activity; Blood coagulation; Echis carinatus; Chromatography; LD50.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100302

Determination of in vivo toxicity and in vitro cytotoxicity of venom from the Cypriot blunt-nosed viper Macrovipera lebetina lebetina and antivenom production
Provedor de dados: 80
Autores: Nalbantsoy,A; Karabay-Yavasoglu,NU; Sayim,F; Deliloglu-Gurhan,I; Gocmen,B; Arikan,H; Yildiz,MZ.
The venomous Levantine viper, Macrovipera lebetina lebetina is endemic to Cyprus. The objective of this study was to investigate in vitro cytotoxicity, in vivo lethality, and antivenom production followed by a re-immunization schedule in mice against Macrovipera lebetina lebetina venom. The LD50 value was estimated as 7.58 mg/kg within 24 hours by different venom doses administrated intraperitoneally in mice. Freund's complete and incomplete adjuvants were used for first and second immunization of mice in antivenom production. A cell-based assay was performed to determine the effects of Macrovipera lebetina lebetina venom and antivenom neutralizing potency on L929 cell viability. The snake venom toxicity and cytotoxicity were examined and comparison of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Macrovipera lebetina lebetina; LD50; Lethality; Snake venom; Antivenom.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200011

Snake venom derived fibrin adhesive in dog uterine healing: biomechanical study
Provedor de dados: 80
Autores: Moraes,J. R. E.; Camplesi,A. C.; Correia,P. H. A.; Shimano,A. C.; Ferraro,G. C.; Moraes,F. R.
Healing is a complex process with many interfering factors. The objective of this work was to evaluate regeneration strength of non-pregnant adult dog uterus when snake venom derived fibrin adhesive is used to reinforce hysterorrhaphy. Maximum limit and rigidity were analyzed. Twenty uterine horns from 10 dogs were hysterotomized and distributed into 2 equal groups. Hysterorrhaphy was performed using the Shimieden-Cushing double layer suture. In one group, animals received snake venom derived fibrin adhesive as reinforcement. Although neither variable was significantly different, our results showed higher rigidity values in the adhesive group. This can be attributed to the adhesive’s effect on organ elasticity or to more granulation tissue formed in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomechanical properties; Healing; Dog uterus; Fibrin adhesive; Snake venom.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200005

Antiviral activity of animal venom peptides and related compounds
Provedor de dados: 80
Autores: Mata,Élida Cleyse Gomes da; Mourão,Caroline Barbosa Farias; Rangel,Marisa; Schwartz,Elisabeth Ferroni.
Abstract Viruses exhibit rapid mutational capacity to trick and infect host cells, sometimes assisted through virus-coded peptides that counteract host cellular immune defense. Although a large number of compounds have been identified as inhibiting various viral infections and disease progression, it is urgent to achieve the discovery of more effective agents. Furthermore, proportionally to the great variety of diseases caused by viruses, very few viral vaccines are available, and not all are efficient. Thus, new antiviral substances obtained from natural products have been prospected, including those derived from venomous animals. Venoms are complex mixtures of hundreds of molecules, mostly peptides, that present a large array of biological activities and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antiretroviral agents; Antiviral agents; HIV; Scorpion venom; Snake venom; Amphibian venom; Insect venom; Marine animal peptides.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100200

Preparation and neutralization efficacy of IgY antibodies raised against Deinagkistrodon acutus venom
Provedor de dados: 80
Autores: Liu,Jinhua; He,Qiyi; Wang,Wenwen; Zhou,Bin; Li,Bo; Zhang,Yingfeng; Luo,Cong; Chen,Diancheng; Tang,Jia; Yu,Xiaodong.
Abstract  Background  The five-paced pit viper (Deinagkistrodon acutus), endemic to China and northern Vietnam, is responsible for most snakebites in the Chinese territory. Antivenom produced from horses is the main treatment for snakebites, but it may cause numerous clinical side effects and have other disadvantages involved in their production such as the welfare of animals. The present study was conducted aiming to develop an alternative antibody (IgY) from the egg yolk of leghorn chickens immunized with snake venom.  Methods  IgY from the egg yolk of white leghorn chickens previously immunized intramuscularly with D. acutus venom was extracted by water, precipitated by ammonium sulfate and purified by affinity chromatographic system. IgY was identified...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IgY antibody; Egg yolk; Snake venom; Snakebite; Deinagkistrodon acutus; Venom neutralization.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100310

Pharmacological and partial biochemical characterization of Bmaj-9 isolated from Bothrops marajoensis snake venom
Provedor de dados: 80
Autores: Galbiatti,C; Rocha,T; Randazzo-Moura,P; Ponce-Soto,LA; Marangoni,S; Cruz-Höfling,MA; Rodrigues-Simioni,L.
Bmaj-9, a basic PLA2 (13679.33 Da), was isolated from Bothrops marajoensis snake venom through only one chromatographic step in reversed phase HPLC on ¼-Bondapak C-18 column. The amino acid composition showed that Bmaj-9 had a high content of Lys, His, and Arg, typical of a basic PLA2. The sequence of Bmaj-9 contains 124 amino acid residues with a pI value of 8.55, such as DLWQWGQMIL KETGKLPFSY YTAYGCYCGW GGRGGKPKAD TDRCCFVHDC, revealing a high homology with Asp49 PLA2 from other snake venoms. It also exhibited a pronounced phospholipase A2 activity when compared with crude venom. In chick biventer cervicis preparations, the time for 50% and 100% neuromuscular paralysis was respectively (in minutes): 110 ± 10 (1 µg/mL); 40 ± 6 and 90 ± 2 (5 µg/mL); 30 ± 3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops marajoensis; Snake venom; Asp49 PLA2; Neuromuscular blockade.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100008

Purification and enzymatic characterization of a novel metalloprotease from Lachesis muta rhombeata snake venom
Provedor de dados: 80
Autores: Cordeiro,Francielle Almeida; Coutinho,Bárbara Marques; Wiezel,Gisele Adriano; Bordon,Karla de Castro Figueiredo; Bregge-Silva,Cristiane; Rosa-Garzon,Nathalia Gonsales; Cabral,Hamilton; Ueberheide,Beatrix; Arantes,Eliane Candiani.
Abstract  Background: Lachesis muta rhombeata (Lmr) is the largest venomous snake in Latin America and its venom contains mainly enzymatic components, such as serine and metalloproteases, L-amino acid oxidase and phospholipases A2. Metalloproteases comprise a large group of zinc-dependent proteases that cleave basement membrane components such as fibronectin, laminin and collagen type IV. These enzymes are responsible for local and systemic changes, including haemorrhage, myonecrosis and inflammation. This study aimed the isolation and enzymatic characterization of the first metalloprotease (Lmr-MP) from Lmr venom (LmrV).  Methods and results: Lmr-MP was purified through two chromatographic steps and submitted to enzymatic characterization. It showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lachesis muta rhombeata; Metalloprotease; Proteases; Snake venom.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100323

New insights into the structural characteristics of irradiated crotamine
Provedor de dados: 80
Autores: Oliveira,Karina Corleto; Spencer,Patrick Jack; Ferreira Jr,Rui Seabra; Nascimento,Nanci.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gamma radiation; Structural modifications; Crotamine; Snake venom; Crotalus durissus terrificus.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100321

Effect of Bothrops alternatus snake venom on macrophage phagocytosis and superoxide production: participation of protein kinase C
Provedor de dados: 80
Autores: Setubal,SS; Pontes,AS; Furtado,JL; Kayano,AM; Stábeli,RG; Zuliani,JP.
Envenomations caused by different species of Bothrops snakes result in severe local tissue damage, hemorrhage, pain, myonecrosis, and inflammation with a significant leukocyte accumulation at the bite site. However, the activation state of leukocytes is still unclear. According to clinical cases and experimental work, the local effects observed in envenenomation by Bothrops alternatus are mainly the appearance of edema, hemorrhage, and necrosis. In this study we investigated the ability of Bothrops alternatus crude venom to induce macrophage activation. At 6 to 100 ¼g/mL, BaV is not toxic to thioglycollate-elicited macrophages; at 3 and 6 ¼g/mL, it did not interfere in macrophage adhesion or detachment. Moreover, at concentrations of 1.5, 3, and 6 ¼g/mL...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops; Macrophages; Phagocytosis; Superoxide; Protein kinase C; Inflammation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400010

Maintaining rear-fanged snakes for venom production: an evaluation of mortality and survival rates for Philodryas olfersii and P. patagoniensis in captivity
Provedor de dados: 80
Autores: Braz,HB; Rocha,MMT; Furtado,MFD.
This study evaluates the mortality and average survival rates of captive female Philodryas olfersii and Philodryas patagoniensis snakes maintained for venom production. Also, two factors likely to reduce captive survival were studied - body condition at admission and seasonality. Mortality peaks occurred during the second month in captivity. More than half the individuals were dead at the end of the third month. This suggests that the first three months in captivity are the most critical in terms of survival and adaptation. Females collected and admitted during spring and summer lived less time than those collected in autumn and winter. As gravidity and egg-laying occur during spring and summer, we suggest that the lower survival rates in these seasons may...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal husbandry; Snake venom; Seasonal variation; Body mass index.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200006

Subproteome of Lachesis muta rhombeata venom and preliminary studies on LmrSP-4, a novel snake venom serine proteinase
Provedor de dados: 80
Autores: Wiezel,Gisele A; Bordon,Karla CF; Silva,Ronivaldo R; Gomes,Mário SR; Cabral,Hamilton; Rodrigues,Veridiana M; Ueberheide,Beatrix; Arantes,Eliane C.
Abstract  Background: Lachesis muta rhombeata is one of the venomous snakes of medical importance in Brazil whose envenoming is characterized by local and systemic effects which may produce even shock and death. Its venom is mainly comprised of serine and metalloproteinases, phospholipases A2 and bradykinin-potentiating peptides. Based on a previously reported fractionation of L. m. rhombeata venom (LmrV), we decided to perform a subproteome analysis of its major fraction and investigated a novel component present in this venom.  Methods: LmrV was fractionated through molecular exclusion chromatography and the main fraction (S5) was submitted to fibrinogenolytic activity assay and fractionated by reversed-phase chromatography. The N-terminal sequences of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bushmaster; Snake venom; SVSP; Kallikrein-like; Plasminogen activator; Kininogenase; Lectin; Protease; Envenomation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100307

Anti-Trimeresurus albolabris venom IgY antibodies: preparation, purification and neutralization efficacy
Provedor de dados: 80
Autores: Duan,Hai-long; He,Qi-yi; Zhou,Bin; Wang,Wen-wen; Li,Bo; Zhang,Ying-zheng; Deng,Qiu-ping; Zhang,Ying-feng; Yu,Xiao-dong.
Abstract  Background  Snakebite incidence in southwestern China is mainly attributed to one of the several venomous snakes found in the country, the white-lipped green pit viper Trimeresurus albolabris. Since antivenom produced from horses may cause numerous clinical side effects, the present study was conducted aiming to develop an alternative antivenom antibody (immunoglobulin Y - IgY) from leghorn chickens.  Methods  IgY in egg yolk from white leghorn chicken previously injected with T. albolabris venom was extracted by water, precipitated by ammonium sulfate and purified by affinity chromatographic system. IgY was identified by SDS-PAGE, ELISA and Western blot, and its neutralizing assay was conducted on mice.  Results  Chickens injected multiple times...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IgY; Egg yolk; Snake venom; Trimeresurus albolabris; LD50.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100316

Effect of fibrin glue derived from snake venom on the viability of autogenous split-thickness skin graft
Provedor de dados: 80
Autores: Rahal,S.C.; Amaral,M.S.P.; Pai,V.D.; Barraviera,S.R.C.S.; Caporal,E.H.G; Crocci,A.J.
The aim of this study was to analyze the effect of snake venom derived from fibrin glue on the viability of split-thickness skin graft. Nine crossbreed dogs were used. Full-thickness skin segments measuring 4 x 4 cm were bilaterally excised from the proximal radial area on each dog. A split-thickness skin graft was harvestedfrom left lateral thoracic area using a freehand graft knife, and was secured to the left recipient bed using several simple interrupted sutures of 3-0 nylon (sutured graft). A split-thickness skin graft was harvested from the right lateral thoracic area using a graft knife. Fibrin glue derived from snake venom was applied to the recipient bed, and 8 equidistant simple interrupted sutures of 3-0 nylon were used to secure the skin graft...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibrin glue; Skin graft; Snake venom; Dogs.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200006

Use of fibrin glue derived from snake venom in the repair of deep corneal ulcers: experimental study in dogs (Canis familiaris, Linnaeus, 1758)
Provedor de dados: 80
Autores: Sampaio,R. L.; Ranzani,J. J. T.; Brandão,C. V. S.; Thomazini-Santos,I. A.; Barraviera,B.; Barraviera,S. R. C. S.; Giannini,M. J. S. M.
Fibrin glue has been researched as an alternative method for tissue synthesis and is known for its capability to promote hemostasis at the application site, good approximation of wound edges and fast healing. The current study consisted in the application of fibrin glue derived from snake venom as treatment for experimental corneal ulcers. Twenty-one dogs had their corneas experimentally prepared through lamellar keratectomy (of standardized diameter and depth). Animals were divided into seven groups of three animals each. Six experimental groups were periodically evaluated and collection was carried out on the 1st, 3rd, 7th, 15th, 30th and 60th post-operative days, whereas one control group was evaluated throughout the experiment. Analyses consisted in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibrin glue; Snake venom; Cornea; Corneal ulcer; Keratoplasty.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400014

Clinical and morphological evaluation of snake venom derived fibrin glue on the tendon healing in dogs
Provedor de dados: 80
Autores: Ferraro,G. C.; Moraes,J. R. E.; Pereira,G. T.; Moraes,F. R.; Bueno de Camargo,M. H.
The aim of this study was to evaluate the effect of snake venom derived fibrin glue on the healing of the deep digital flexor tendon, during three periods. The tendon of the 2nd digit of 30 thoracic limbs of dogs was partially sectioned for glue application. Biopsies were performed 7, 15, and 30 days post surgery for the clinical and morphological study of tendons. Analysis of the results showed that 73.3% of the tendons showed stump retraction and 16.6% moderate to excessive adherence, which affected sliding. There was a significant difference in the number of inflammatory cells among the three studied periods, being the highest on day 15. The morphological analysis revealed a typical tendon healing process with a lower level of inflammation in the acute...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dog; Fibrin glue; Snake venom; Healing; Tendon.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400005

Protein profile analysis of Malaysian snake venoms by two-dimensional gel electrophoresis
Provedor de dados: 80
Autores: Vejayan,J; Shin Yee,L; Ponnudurai,G; Ambu,S; Ibrahim,I.
Snake venoms comprise a highly complex mixture of proteins, which requires for their characterization the use of versatile two-dimensional electrophoresis techniques. In the present study, venoms obtained from eight snakes (Ophiophagus hannah, Naja kaouthia, Naja sumatrana, Bungarus fasciatus, Trimeresurus sumatranus, Tropidolaemus wagleri, Enhydrina schistosa and Calloselasma rhodostoma) commonly found in Malaysia were separated based on two independent properties, isoelectric point (pI) and molecular weight (MW). Many differences in snake venoms at the inter-family, inter-subfamily, inter-genus and inter-species levels were revealed. Notably, proteins from individuals of the Viperidae family - Trimeresurus sumatranus, Tropidolaemus wagleri and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Protein profile analysis; Two-dimensional gel electrophoresis; SDS-PAGE.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400013

Mechanisms of virus resistance and antiviral activity of snake venoms
Provedor de dados: 80
Autores: Rivero,JVR; de Castro,FOF; Stival,AS; Magalhães,MR; Carmo Filho,JR; Pfrimer,IAH.
Viruses depend on cell metabolism for their own propagation. The need to foster an intimate relationship with the host has resulted in the development of various strategies designed to help virus escape from the defense mechanisms present in the host. Over millions of years, the unremitting battle between pathogens and their hosts has led to changes in evolution of the immune system. Snake venoms are biological resources that have antiviral activity, hence substances of significant pharmacological value. The biodiversity in Brazil with respect to snakes is one of the richest on the planet; nevertheless, studies on the antiviral activity of venom from Brazilian snakes are scarce. The antiviral properties of snake venom appear as new promising therapeutic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Viruses; Antiviral agents.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400005

In vitro hemolytic activity of Bothrops lanceolatus (fer-de-lance) venom
Provedor de dados: 80
Autores: Martins,LJ; de Araújo,PMF; Bon,C; Hyslop,S; de Araújo,AL.
Bothrops lanceolatus venom contains a variety of enzymatic and biological activities. The present work investigated the hemolytic activity of this venom and its phospholipase A2 (PLA2). Bothrops lanceolatus venom (6.7 µg/mL) caused indirect hemolysis of cow, horse, rat and sheep erythrocytes, with horse erythrocytes being the most sensitive; no direct hemolysis was observed. Hemolysis in sheep erythrocytes was concentration-dependent (5-11.7 µg/mL) and markedly attenuated by heating the venom for 30 minutes at ≥ 40°C and by the PLA2 inhibitor p-bromophenacyl bromide. An acidic PLA2 (5 µg/mL) purified from B. lanceolatus venom also caused hemolysis. This PLA2 showed immunoprecipitin lines with antivenom against B. lanceolatus, which suggests that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops lanceolatus; Hemolytic activity; Phospholipase A2; Snake venom.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300011

Tumor cytotoxicity of leucurolysin-B, a P-III snake venom metalloproteinase from Bothrops leucurus
Provedor de dados: 80
Autores: Gabriel,LM; Sanchez,EF; Silva,SG; Santos,RG.
Although it has been demonstrated that venoms and toxins from some snakes are able to influence the growth of tumor cells, few antitumoral compounds from Bothrops leucurus venom have been characterized. Leucurolysin-B (leuc-B) is a metalloproteinase class P-III isolated from B. leucurus which possesses an ECD-disintegrin domain. Both ECD-disentegrin and RGD-disintegrin are able to bind to cell surface integrins and inhibit their adherence to their natural ligands. In the present study, the potential efficacy and the cytotoxic effects of leuc-B on glioblastoma, breast cancer and melanoma cell lines were analyzed. The effect of leuc-B on cancer cell survival was evaluated and its 50% inhibitory concentration (IC50) was determined. Morphological alterations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Leucurolysin-B; ECD-disintegrin; Tumor; In vitro study.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100004

Cross-neutralization of the coagulant activity of Crotalus durissus terrificus venom from the northeast of Argentina by bivalent bothropic antivenom
Provedor de dados: 80
Autores: Rodríguez,JP; Gay,CC; Fusco,LS; Gauna,MC; Acosta,OC; Leiva,LC.
Cross-neutralization of Crotalus durissus terrificus venom coagulant activity was tested using bivalent horse antivenom against Bothrops alternatus and Bothrops diporus venoms. Our in vitro and in vivo experiments showed that bothropic antivenom neutralizes the thrombin-like activity of crotalic snake venom and this cross-reaction was demonstrated by immunoassays either with whole venom or a purified thrombin-like enzyme. These results suggest common antigenic properties and, consequently, similar molecular structure among venom thrombin-like enzymes. Besides, they provide information that could be further used in the development of new antivenom formulations.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thrombin-like enzyme; Snake venom; Antivenoms; Immunological cross-reaction.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100015

Efficacy of tannins from Mimosa pudica and tannic acid in neutralizing cobra (Naja kaouthia) venom
Provedor de dados: 80
Autores: Sia,FY; Vejayan,J; Jamuna,A; Ambu,S.
In the present study, the effectiveness of Mimosa pudica tannins (MPT) in neutralizing the lethality of Naja kaouthia venom was compared with commercially derived tannins. Preincubation of MPT with N. kaouthia venom maintained 100% survival of mice after 24 hours. The mouse group in which there was no preincubation, no protection against the effects of the venom was observed. M. pudica tannin was found to be more effective in neutralizing the lethality of N. kaouthia venom when compared to commercial tannic acid. Two protein spots were missing in the two-dimensional gel electrophoresis (2-DE) of the MPT treated mouse indicating the down-regulation of venom proteins. The results from this study indicated that tannins obtained from M. pudica are better than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant extract; Snake venom; Therapy; Tannin; Phospholipase A2.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100006

Enzymatic and biochemical characterization of Bungarus sindanus snake venom acetylcholinesterase
Provedor de dados: 80
Autores: Ahmed,M; Latif,N; Khan,RA; Ahmad,A; Rocha,JBT; Mazzanti,CM; Bagatini,MD; Morsch,VM; Schetinger,MRC.
This study analyses venom from the elapid krait snake Bungarus sindanus, which contains a high level of acetylcholinesterase (AChE) activity. The enzyme showed optimum activity at alkaline pH (8.5) and 45ºC. Krait venom AChE was inhibited by substrate. Inhibition was significantly reduced by using a high ionic strength buffer; low ionic strength buffer (10 mM PO4 pH 7.5) inhibited the enzyme by 1. 5mM AcSCh, while high ionic strength buffer (62 mM PO4 pH 7.5) inhibited it by 1 mM AcSCh. Venom acetylcholinesterase was also found to be thermally stable at 45ºC; it only lost 5% of its activity after incubation at 45ºC for 40 minutes. The Michaelis-Menten constant (Km) for acetylthiocholine iodide hydrolysis was found to be 0.068 mM. Krait venom...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetylcholinesterase; Inhibition; Krait; Ionic strength; Acetylthiocholine iodide; Bungarus sindanus; Snake venom.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200014

Effect of snake venom derived fibrin glue on the tendon healing in dogs: clinical and biomechanical study
Provedor de dados: 80
Autores: Ferraro,G. C.; Moraes,J. R.; Shimano,A. C.; Pereira,G. T.; Moraes,F. R.; Bueno de Camargo,M. H.
The aim of this study was to evaluate the effect of snake venom derived fibrin glue on the strength oftendon healing in dogs. The deep digital flexor tendon of the 5th digit of 24 thoracic limbs was partially sectioned for adhesive application. On the 7th, 15th, and 30th postoperative day tendons segments were removed for the clinical and biomechanical study. Results indicated that 62.5% of the tendons showed stump retraction and 20.8% moderate to excessive adherence, which affected gliding. The biomechanical evaluation showed that, over time, tendon healing gained progressive resistance for maximum traction and permanent deformations with satisfactory results on the 15th day for rigidity and resilience compared to the other two studied periods. Snake...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Fibrin glue; Mechanical properties; Snake venom; Healing; Tendon.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300005

Development of a sensitive enzyme immunoassay (ELISA) for specific identification of Lachesis acrochorda venom
Provedor de dados: 80
Autores: Núñez Rangel,V; Fernández Culma,M; Rey-Suárez,P; Pereañez,JA.
The snake genus Lachesis provokes 2 to 3% of snakebites in Colombia every year. Two Lachesis species, L. acrochorda and L. muta, share habitats with snakes from another genus, namely Bothrops asper and B. atrox. Lachesis venom causes systemic and local effects such as swelling, hemorrhaging, myonecrosis, hemostatic disorders and nephrotoxic symptoms similar to those induced by Bothrops, Portidium and Bothriechis bites. Bothrops antivenoms neutralize a variety of Lachesis venom toxins. However, these products are unable to avoid coagulation problems provoked by Lachesis snakebites. Thus, it is important to ascertain whether the envenomation was caused by a Bothrops or Lachesis snake. The present study found enzyme linked immunosorbent assay (ELISA)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Lachesis; Lachesis acrochorda; Enzyme-linked immunosorbent assay.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200007

Antiophidian properties of plant extracts against Lachesis muta venom
Provedor de dados: 80
Autores: De Paula,RC; Sanchez,EF; Costa,TR; Martins,CHG; Pereira,PS; Lourenço,MV; Soares,AM; Fuly,AL.
Snakebites comprise a serious health problem in several countries due to their global incidence, which exceeds 2.5 million per year, and the elevated number of victim fatalities. To counteract envenomations, antivenoms have been used regularly for more than a century. Apart from side effects including anaphylactic reactions, antivenoms are not able to efficiently neutralize local tissue damage, which contributes to increasing the severity and morbidity observed in patients. This fact, in turn, may be responsible for economic hardship, particularly in rural populations of developing countries. In the present work, we evaluated the antiophidian properties of 12 Brazilian plant extracts against the hemolytic, coagulant, hemorrhagic and proteolytic effects of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Lachesis muta; Plant extract; Antivenom; Biological activities.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200012

BjussuLAAO-II induces cytotoxicity and alters DNA methylation of cell-cycle genes in monocultured/co-cultured HepG2 cells
Provedor de dados: 80
Autores: Machado,Ana Rita Thomazela; Aissa,Alexandre Ferro; Ribeiro,Diego Luis; Ferreira Jr.,Rui Seabra; Sampaio,Suely Vilela; Antunes,Lusânia Maria Greggi.
Abstract  Background: The use of animal venoms and their toxins as material sources for biotechnological applications has received much attention from the pharmaceutical industry. L-amino acid oxidases from snake venoms (SV-LAAOs) have demonstrated innumerous biological effects and pharmacological potential against different cancer types. Hepatocellular carcinoma has increased worldwide, and the aberrant DNA methylation of liver cells is a common mechanism to promote hepatic tumorigenesis. Moreover, tumor microenvironment plays a major role in neoplastic transformation. To elucidate the molecular mechanisms responsible for the cytotoxic effects of SV-LAAO in human cancer cells, this study aimed to evaluate the cytotoxicity and the alterations in DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Epigenetics; GADD45A; CCND1; CDKN1A.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100305

Bothrops snake venoms and their isolated toxins, an L-amino acid oxidase and a serine protease, modulate human complement system pathways
Provedor de dados: 80
Autores: Ayres,Lorena Rocha; Récio,Alex dos Reis; Burin,Sandra Mara; Pereira,Juliana Campos; Martins,Andrea Casella; Sampaio,Suely Vilela; Castro,Fabíola Attié de; Pereira-Crott,Luciana Simon.
Background Activation of the complement system plays an important role in the regulation of immune and inflammatory reactions, and contributes to inflammatory responses triggered by envenomation provoked byBothrops snakes. The present study aimed to assess whether Bothrops jararacussuand Bothrops pirajai crude venoms and their isolated toxins, namely serine protease (BjussuSP-I) and L-amino acid oxidase (BpirLAAO-I), modulate human complement system pathways.Methods Lyophilized venom and toxin samples solubilized in phosphate buffered saline were diluted in appropriate buffers to evaluate their hemolytic activity on the alternative and classical pathways of the complement system. Venom- and toxin-treated normal human serum was added to the erythrocyte...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararacussu; Bothrops pirajai; Chemotaxis; Complement system; Kinetic microassay; L-amino acid oxidase; Serine protease; Snake venom.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100336

The red seaweed Plocamium brasiliense shows anti-snake venom toxic effects
Provedor de dados: 80
Autores: Silva,Geisiane Alves da; Domingos,Thaisa Francielle Souza; Fonseca,Rainiomar Raimundo; Sanchez,Eladio Flores; Teixeira,Valéria Laneuville; Fuly,André Lopes.
Background Snakebite is considered a neglected tropical disease by the World Health Organization. In Brazil, about 70% of the envenomation cases are caused by Bothrops snakes. Its venom may provoke hemorrhage, pain, necrosis, hemolysis, renal or cardiac failure and even death in victims. Since commercial antivenom does not efficiently neutralize the local toxic effects of venoms, natural products have been tested in order to provide alternative or complementary treatment to serum therapy. Therefore, the present study aimed to evaluate the ability of the seaweed Plocamium brasiliense and its active derivatives to neutralize hemorrhagic, edematogenic, hemolytic, coagulant and proteolytic activities of B. jararaca venom. Methods Specimens of P. brasiliense...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararaca; Snake venom; Plocamium brasiliense; Seaweed; Antivenom; Bioprospecting.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100312

Kinetic investigations and stability studies of two Bothrops L-amino acid oxidases
Provedor de dados: 80
Autores: Costa,Tássia R.; Carone,Sante E. I.; Tucci,Luiz F. F.; Menaldo,Danilo L.; Rosa-Garzon,Nathalia G.; Cabral,Hamilton; Sampaio,Suely V.
Abstract  Background: L-amino acid oxidases isolated from snake venoms (SV-LAAOs) are enzymes that have great therapeutic potential and are currently being investigated as tools for developing new strategies to treat various diseases, including cancer and bacterial infections. The main objective of this study was to make a brief evaluation of the enzymatic stability of two Bothrops LAAOs, one isolated from Bothrops jararacussu (BjussuLAAO-II) and the other from Bothrops moojeni (BmooLAAO-I) venoms.  Methods and results: The enzymatic activity and stability of both LAAOs were evaluated by microplate colorimetric assays, for which BjussuLAAO-II and BmooLAAO-I were incubated with different L-amino acid substrates, in the presence of different ions, and at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops; L-amino acid oxidase; Enzymatic stability.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100327

A bradykinin-potentiating peptide (BPP-10c) from Bothrops jararaca induces changes in seminiferous tubules
Provedor de dados: 80
Autores: Gilio,Joyce M; Portaro,Fernanda CV; Borella,Maria I; Lameu,Claudiana; Camargo,Antonio CM; Alberto-Silva,Carlos.
Background The testis-specific isoform of angiotensin-converting enzyme (tACE) is exclusively expressed in germ cells during spermatogenesis. Although the exact role of tACE in male fertility is unknown, it clearly plays a critical function in spermatogenesis. The dipeptidase domain of tACE is identical to the C-terminal catalytic domain of somatic ACE (sACE). Bradykinin potentiating peptides (BPPs) from snake venoms are the first natural sACE inhibitors described and their structure-activity relationship studies were the basis for the development of antihypertensive drugs such as captopril. In recent years, it has been showed that a number of BPPs - including BPP-10c - are able to distinguish between the N- and C-active sites of sACE, what is not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bradykinin-potentiating peptide; Snake venom; Angiotensin-converting enzyme; Testis; Seminiferous epithelium.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100316

Bordonein-L, a new L-amino acid oxidase from Crotalus durissus terrificus snake venom: isolation, preliminary characterization and enzyme stability
Provedor de dados: 80
Autores: Bordon,Karla C. F.; Wiezel,Gisele A.; Cabral,Hamilton; Arantes,Eliane C.
BackgroundCrotalus durissus terrificus venom (CdtV) is one of the most studied snake venoms in Brazil. Despite presenting several well known proteins, its L-amino acid oxidase (LAAO) has not been studied previously. This study aimed to isolate, characterize and evaluate the enzyme stability of bordonein-L, an LAAO from CdtV.Methods The enzyme was isolated through cation exchange, gel filtration and affinity chromatography, followed by a reversed-phase fast protein liquid chromatography to confirm its purity. Subsequently, its N-terminal amino acid sequence was determined by Edman degradation. The enzyme activity and stability were evaluated by a microplate colorimetric assay and the molecular mass was estimated by SDS-PAGE using periodic acid-Schiff...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Crotalus durissus terrificus; L-amino acid oxidase; Rattlesnake; Enzyme activity; Enzyme stability; Chromatography; Snake venom; Yellow venom; Stabilization.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100335

Autologous or heterologous fibrin sealant scaffold: which is the better choice?
Provedor de dados: 80
Autores: Ferreira,Rui Seabra.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Fibrin sealant; Snake venom; Scaffold; Stem cell.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200103

General characterization of venom from the Moroccan snakes Macrovipera mauritanica and Cerastes cerastes
Provedor de dados: 80
Autores: Oukkache,N; Lalaoui,M; Ghalim,N.
Ophidian envenomation accidents constitute a serious public health problem in many countries around the globe. Over 5 million such accident cases occur each year causing more than 100,000 deaths. In Africa, more than 20,000 deaths per year are registered while 400,000 envenomation victims retain severe and permanent functional sequelae. In Morocco, snakebites are frequent and of greater severity in children. They occur mostly in rural areas. The incidence of these bites remains poorly understood and vastly underestimated. The epidemiological data are not well known due to the absence of a national registry, whereas a significant proportion of envenomations receive only traditional treatment methods in non-medical intensive care. This prompted us to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerastes cerastes; Macrovipera mauritanica; Snake venom; Characterization; Biological activities; Cross-reaction.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400009

Venomics and antivenomics of the poorly studied Brazil’s lancehead, Bothrops brazili (Hoge, 1954), from the Brazilian State of Pará
Provedor de dados: 80
Autores: Sanz,Libia; Pérez,Alicia; Quesada-Bernat,Sarai; Diniz-Sousa,Rafaela; Calderón,Leonardo A.; Soares,Andreimar M.; Calvete,Juan J.; Caldeira,Cleópatra A. S.
ABSTRACT  Background: The Brazil’s lancehead, Bothrops brazili, is a poorly studied pit viper distributed in lowlands of the equatorial rainforests of southern Colombia, northeastern Peru, eastern Ecuador, southern and southeastern Venezuela, Guyana, Suriname, French Guiana, Brazil, and northern Bolivia. Few studies have been reported on toxins isolated from venom of Ecuadorian and Brazilian B. brazili. The aim of the present study was to elucidate the qualitative and quantitative protein composition of B. brazili venom from Pará (Brazil), and to carry out a comparative antivenomics assessment of the immunoreactivity of the Brazilian antibothropic pentavalent antivenom [soro antibotrópico (SAB) in Portuguese] against the venoms of B. brazili and reference...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops brazili; Venomics; Third-generation antivenomics; Brazilian antibothropic polyvalent antivenom.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100308

Purification and functional characterization of two fibrinogenolytic enzymes from Bothrops alternatus venom
Provedor de dados: 80
Autores: Costa,J. O.; Petric,C. B.; Hamaguchi,A.; Homsi-Brandeburgo,M. I.; Oliveira,C. Z.; Soares,A. M.; Oliveira,F.
Two fibrinogenolytic enzymes, Bothrops alternatus metalloprotease isoform (BaltMP)-I and II, were purified from Bothrops alternatus venom using Diethylaminoethyl (DEAE) Sephacel, Sephadex G-75 and Heparin-Agarose column chromatography. Purified BaltMP-I and II ran as single protein bands on analytical polyacrylamide gel electrophoresis and showed molecular weights of 29000 and 36000, respectively, under reducing conditions in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). BaltMP-II, but not BaltMP-I, displayed blood-clotting activity in bovine plasma, which was about 10-fold higher than that of the crude venom. Both enzymes were proteolytically active against bovine fibrinogen as substrate. When fibrinogen and each enzyme were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops alternatus; Metalloproteases; Functional characterization; Fibrinogenolytic activity; Defibrinogenation in vivo.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300007

Bothrops alternatus snake venom induces apoptosis of skeletal muscle cells in a rabbit model
Provedor de dados: 45
Autores: Santos,W.G.; Silveira,T.L.; Fiúza,A.T.L.; Botelho,A.F.M.; Gonçalves,I.N.N.; Ferreira,E.; Soto-Blanco,B.; Melo,M.M.
RESUMO Este estudo objetiva reportar a ocorrência de apoptose in vivo induzida pelo veneno da serpente Bothrops alternatus em células musculares esqueléticas. Cinco coelhos machos, adultos, receberam 150μg/kg de veneno no músculo vasto lateral, enquanto outros cinco animais receberam 0,1% de BSA diluído em PBS no mesmo local. Após 12 horas, os animais foram eutanasiados, e amostras do local de inoculação foram coletadas para análise histopatológica. Foram evidenciadas necrose e hemorragia nas células musculares. Além disso, a análise imuno-histoquímica para identificação de caspase-3 ativada revelou marcações granulares e agregadas no citoplasma das células musculares, compatíveis com o processo de apoptose. Este é o primeiro relato que confirma o veneno...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Snake venom; Botropic venom; Apoptosis; Toxicology; Rabbit.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100349

Isolation and biological characterization of a basic phospholipase A2 from Bothrops jararacussu snake venom
Provedor de dados: 47
Autores: Maruñak,S.L.; Leiva,L; Garcia Denegri,M.E.; Teibler,P; Acosta De Pérez,O.
A phospholipase A2 has been isolated from Bothrops jararacussu venom from snakes that inhabit the northeast region of Argentina. The present study describes in vivo and in vitro biological activities of phospholipase A2 from B. jararacussu as well as isolation details of its. Venom was obtained by milking of adult snakes which were housing in wood reptile cages of varying dimensions in heated (20-30ºC) rooms. Snakes received a weekly diet of mice and water was available ad libitum for drinking and soaking. The enzyme was purified by gel filtration on a Sephadex G-75 column followed by ion exchange chromatography on a SP-Sephadex C25 column. The major peak belonging to proteins was retained in the cation exchanger and then eluted using a concentration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops jararacussu; Phospholipase A2; Isolation.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300001

Effect of suramin on myotoxicity of some crotalid snake venoms
Provedor de dados: 56
Autores: Arruda,E.Z.; Silva,N.M.V.; Moraes,R.A.M.; Melo,P.A.
We investigated the protective effect of suramin, an enzyme inhibitor and an uncoupler of G protein from receptors, on the myotoxic activity in mice of different crotalid snake venoms (A.c. laticinctus, C.v. viridis, C.d. terrificus, B. jararacussu, B. moojeni, B. alternatus, B. jararaca, L. muta). Myotoxicity was evaluated in vivo by injecting im the venoms (0.5 or 1.0 mg/kg) dissolved in physiological saline solution (0.1 ml) and measuring plasma creatine kinase (CK) activity. Two experimental approaches were used in mice (N = 5 for each group). In protocol A, 1 mg of each venom was incubated with 1.0 mg suramin (15 min, 37ºC, in vitro), and then injected im into the mice at a dose of 1.0 mg/kg (in vivo). In protocol B, venoms, 1.0 mg/kg, were injected...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Snake venom; Myotoxicity; Creatine kinase; Suramin.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600013

Anti-ophidian activity of Bredemeyera floribunda Willd. (Polygalaceae) root extract on the local effects induced by Bothrops jararacussu venom
Provedor de dados: 56
Autores: Alves,N.T.Q.; Ximenes,R.M.; Jorge,R.J.B.; Silveira,J.A.M.; Santos,J.V.A.; Rodrigues,F.A.P.; Costa,P.H.S.; Xavier Jr.,F.A.F.; Evangelista,J.S.A.M.; Havt,A.; Soares,V.C.G.; Toyama,M.H.; Oliveira,A.N.A.; Araújo,R.M.; Alves,R.S.; Monteiro,H.S.A.
Bredemeyera floribunda roots are popularly used to treat snakebites in the semiarid region of Northeast Brazil, and previous studies indicate the anti-ophidian actions of triterpenoid saponins found in its roots. To assess B. floribunda root extract (BFRE) activity against the effects of Bothrops jararacussu venom (BjuV), antiphospholipasic, antiproteolytic, antihemorrhagic, antinecrotic, and anti-edematogenic activities were investigated in mice. Phytochemical analysis revealed the presence of saponins, flavonoids, and sugars, with rutin and saccharose being the major constituents of BFRE. Acute toxicity was determined and BFRE was nontoxic to mice. Phospholipase A2 and proteolytic activities induced by BjuV were inhibited in vitro by BFRE at all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bredemeyera floribunda; Snakeroot; Snake venom; Rutin; Antihemorrhagic; Anti-edematogenic.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100608

Distribution of 131I-labeled Bothrops erythromelas venom in mice
Provedor de dados: 56
Autores: Vasconcelos,C.M.L.; Valença,R.C.; Araújo,E.A.; Modesto,J.C.A.; Pontes,M.M.; Brazil,T.K.; Guarnieri,M.C.
Bothrops erythromelas is responsible for many snake bites in northeastern Brazil. In the present study we determined the in vivo distribution of the venom following its subcutaneous injection into mice. B. erythromelas venom and albumin were labeled individually with 131I by the chloramine T method, and separated in a Sephacryl® S-200 column. The efficiency of labeling was 68%. Male Swiss mice (40-45 g), which had been provided with drinking water containing 0.05% KI over a period of 10 days prior to the experiment, were inoculated dorsally (sc) with 0.3 ml (2.35 x 105 cpm/mouse) of 131I-venom (N = 42), 131I-albumin or 131I (controls, N = 28 each). Thirty minutes and 1, 3, 6, 12, 18 and 24 h after inoculation, the animals were perfused with 0.85% NaCl and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops erythromelas; Snake venom; Protein iodination.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300017

Neutralizing capacity of a new monovalent anti-Bothrops atrox antivenom: comparison with two commercial antivenoms
Provedor de dados: 56
Three horse-derived antivenoms were tested for their ability to neutralize lethal, hemorrhagic, edema-forming, defibrinating and myotoxic activities induced by the venom of Bothrops atrox from Antioquia and Chocó (Colombia). The following antivenoms were used: a) polyvalent (crotaline) antivenom produced by Instituto Clodomiro Picado (Costa Rica), b) monovalent antibothropic antivenom produced by Instituto Nacional de Salud-INS (Bogotá), and c) a new monovalent anti-B. atrox antivenom produced with the venom of B. atrox from Antioquia and Chocó. The three antivenoms neutralized all toxic activities tested albeit with different potencies. The new monovalent anti-B. atrox antivenom showed the highest neutralizing ability against edema-forming and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bothrops atrox; Snake venom; Antivenom; Neutralization; Antioquia; Chocó.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300011

Behavioral effects of Bj-PRO-7a, a proline-rich oligopeptide from Bothrops jararaca venom
Provedor de dados: 56
Autores: Turones,L.C.; Cruz,K.R. da; Camargo-Silva,G.; Reis-Silva,L.L.; Graziani,D.; Ferreira,P.M.; Galdino,P.M.; Pedrino,G.R.; Santos,R.; Costa,E.A.; Ianzer,D.; Xavier,C.H.
The heptapeptide Bj-PRO-7a, isolated and identified from Bothrops jararaca (Bj) venom, produces antihypertensive and other cardiovascular effects that are independent on angiotensin converting enzyme inhibition, possibly relying on cholinergic muscarinic receptors subtype 1 (M1R). However, whether Bj-PRO-7a acts upon the central nervous system and modifies behavior is yet to be determined. Therefore, the aims of this study were: i) to assess the effects of acute administration of Bj-PRO-7a upon behavior; ii) to reveal mechanisms involved in the effects of Bj-PRO-7a upon locomotion/exploration, anxiety, and depression-like behaviors. For this purpose, adult male Wistar (WT, wild type) and spontaneous hypertensive rats (SHR) received intraperitoneal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bj-PRO-7a; Snake venom; Neuroactive compounds; Anxiety; Depression; Behavior.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001100606

Isolation and characterization of a serine proteinase with thrombin-like activity from the venom of the snake Bothrops asper
Provedor de dados: 56
Autores: Pérez,A.V; Rucavado,A; Sanz,L; Calvete,J.J; Gutiérrez,J.M.
A serine proteinase with thrombin-like activity was isolated from the venom of the Central American pit viper Bothrops asper. Isolation was performed by a combination of affinity chromatography on aminobenzamidine-Sepharose and ion-exchange chromatography on DEAE-Sepharose. The enzyme accounts for approximately 0.13% of the venom dry weight and has a molecular mass of 32 kDa as determined by SDS-PAGE, and of 27 kDa as determined by MALDI-TOF mass spectrometry. Its partial amino acid sequence shows high identity with snake venom serine proteinases and a complete identity with a cDNA clone previously sequenced from this species. The N-terminal sequence of the enzyme is VIGGDECNINEHRSLVVLFXSSGFL CAGTLVQDEWVLTAANCDSKNFQ. The enzyme induces clotting of plasma...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Snake venom; Bothrops asper; Serine proteinase; Thrombin-like serine proteinase; Defibrin(ogen)ation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000100003

Alternagin-C, a disintegrin-like protein from the venom of Bothrops alternatus, modulates alpha2ß1 integrin-mediated cell adhesion, migration and proliferation
Provedor de dados: 56
Autores: Selistre-de-Araujo,H.S.; Cominetti,M.R.; Terruggi,C.H.B.; Mariano-Oliveira,A.; De Freitas,M.S.; Crepin,M.; Figueiredo,C.C.; Morandi,V.
The alpha2ß1 integrin is a major collagen receptor that plays an essential role in the adhesion of normal and tumor cells to the extracellular matrix. Alternagin-C (ALT-C), a disintegrin-like protein purified from the venom of the Brazilian snake Bothrops alternatus, competitively interacts with the alpha2ß1 integrin, thereby inhibiting collagen binding. When immobilized in plate wells, ALT-C supports the adhesion of fibroblasts as well as of human vein endothelial cells (HUVEC) and does not detach cells previously bound to collagen I. ALT-C is a strong inducer of HUVEC proliferation in vitro. Gene expression analysis was done using an Affimetrix HU-95A probe array with probe sets of ~10,000 human genes. In human fibroblasts growing on collagen-coated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alpha2ß1 integrin; Disintegrin; Snake venom; Adhesion; Gene expression; Extracellular matrix.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000007