Consulta: subjectFacets:"Somatic embryos"
Registros recuperados: 9
Data/hora: 06/06/2024 01:35:16

An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis
Provedor de dados: 69
Autores: Sharry,Sandra; Cabrera Ponce,José Luis; Herrera Estrella,Luis; Rangel Cano,Rosa María; Lede,Silvia; Abedini,Walter.
A highly efficient somatic embryogenesis system and subsequent plant regeneration of chinaberry (Melia azedarach L.) was developed. Plants were regenerated from indirect somatic embryogenesis induction. Novel features of this improved protocol, include: a) Embryogenic callus induction with no addition of 2, 4-D in the culture media; b) Somatic embryos differentiation was achieved by using high concentration of cytokinins (BAP 10 mg/L) and adenine; c) 100% conversion of somatic embryos to plants was practically obtained and 100% of plants survived under greenhouse conditions; d) Addition of putrescine improved somatic embryos germination. The amount of somatic embryos produced by the pathway of indirect somatic embryogenesis was 447 per gram of fresh weight...
Tipo: Journal article
Palavras-chave: Callus induction; Clonal forestry; Embryos conversion; Micropropagation; Persian lilac; Somatic embryos.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300003

Preservation of endangered Tunisian grapevine cultivars using embryogenic cultures
Provedor de dados: 69
Autores: Bouamama,Badra; Jardak,Rahma; Salem,Asma Ben; Ghorbel,Abdelwahed; Mliki,Ahmed.
The preservation of embryogenic lines derived from several endangered local grapevine cultivars was studied. Embryogenic calluses were obtained from immature anthers of eight cultivars, sampled on both fruity-cuttings and field grown vines. Anthers at the 'separated flower' stage, derived from fruity-cuttings, resulted in an increased induction of somatic embryogenesis, compared to those derived from the field. Pro-embryogenic calluses were induced on Chée and Pool (1987) basal medium, supplemented with 9 µM of 2,4-dichlorophenoxyacetic acid (2,4-D) and 11.35 µM of thidiazuron (TDZ) under dark conditions. Different anther zones (filament, abaxial, adaxial, lateral zones and entire anthers) were involved in somatic embryogenesis induction. The percentages...
Tipo: Journal article
Palavras-chave: Forced anthers; Long-term maintenance; Somatic embryos; Vitis vinifera.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200006

Direct somatic embryogenesis and organogenesis pathway of plant regeneration can seldom occur simultaneously within the same explant of sorghum
Provedor de dados: 30
Autores: Girijashankar, V.
Palavras-chave: Somatic embryos; Transgenics; Somatic embryogenesis; Auxins; Regeneration; Protocols; Sorghum; Cytokinins; Embryonic development; Transgenic plants.
Ano: 2007
URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3300

Histological changes in banana explants, cv. Nanicão (Musa spp., Group AAA), submitted to different auxins for induction of somatic embryogenesis
Provedor de dados: 88
Autores: FILIPPI,SILVIA BALBÃO; APPEZZATO-DA-GLÓRIA,BEATRIZ; RODRIGUEZ,ADRIANA PINHEIRO MARTINELLI.
The present work analyzes the behavior of banana explants, cv. Nanicão (Musa spp. Group AAA) regarding somatic embryogenesis induction treatments with several auxins. Longitudinal segments of shoot meristematic apices of micropropagated banana plantlets cultivated and rooted in vitro were introduced in culture medium containing dicamba, picloram, 2,4-D or NAA in different concentrations. Explant samples were collected at 0, 7 and 10 days and prepared for light microscopy. Histological sections were used for comparison of the histological changes occurring after induction treatment with different auxins. Embryogenic response was observed only in treatments with picloram or dicamba, with distinct embryogenic regions observed at 14 and 21 days in culture,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Histology; Somatic embryos; Dicamba; Picloram; Banana.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000500015

Plant regeneration and histological study of the somatic embryogenesis of sugarcane (Saccharum spp.) cultivars RB855156 and RB72454 - doi: 10.4025/actasciagron.v36i1.16342
Provedor de dados: 1
Autores: Alcantara, Giovana Bomfim de; Universidade Federal do Paraná; Dibax, Roberson; Universidade Federal do Paraná; Bespalhok Filho, João Carlos; Universidade Federal do Paraná; Daros, Edelclaiton; Universidade Federal do Paraná.
The development of an efficient regeneration system is crucial for the genetic transformation of sugarcane. The aims of this study were to regenerate plants from somatic embryos and analyze the origin and division pattern of cells during the different embryonic developmental stages of the sugarcane cultivars RB855156 and RB72454. For both cultivars, the best results for embryogenic callus induction were obtained with explant cultures on culture medium containing 13.5 µM 2,4-dichlorophenoxyacetic acid (2,4-D) followed by callus culture on solid medium with 4.5 µM 2,4-D. A higher rate of shoot induction was observed in RB855156 with the addition of 8.9 and 17.8 µM benzylaminopurine (BAP); this higher rate was also observed in RB72454 with the addition of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.00.00.00-4; 5.01.03.00-8; 5.01.03.05-9; Tissue culture; Somatic embryos; Anatomy; 2 4-D; Poaceae Ciências Agrárias  Fitotecnia  Melhoramento Vegetal.
Ano: 2014
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/16342

Somatic embryogenesis and plant regeneration from callus cultures of Cleome rosea Vahl
Provedor de dados: 52
Autores: Simões,Claudia; Albarello,Norma; Callado,Cátia Henriques; Castro,Tatiana Carvalho de; Mansur,Elisabeth.
This paper describes a protocol for the efficient vegetative propagation of Cleome rosea by somatic embryogenesis. Leaf and stem explants from nursery-grown seedlings of C. rosea were cultivated on Murashige and Skoog (MS) medium supplemented with indole-3-acetic acid (IAA), a -naphthaleneacetic acid (NAA), 4-amino-3,5,6-trichloropicolinic acid (picloram) or 2,4-dichlorophenoxyacetic acid (2,4-D). Nodular calli were produced from both explant types in the presence of 4.5 and 9.0 µM 2,4-D. Embryo development and maturation were achieved when calli from stem explants were transferred to media containing a ten-fold reduction of 2,4-D concentration initially used (0.45 and 0.90 µM). Leaf-derived calli did not form embryos with the same treatments. The highest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Auxin; Embriogenic callus; Histology; Phytagel; Somatic embryos.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300024

Influence of Silver Nitrate on Somatic Embryogenesis Induction in Arabica Coffee (Coffea arabica L.)
Provedor de dados: 52
Autores: Rojas-Lorz,Laura; Arrieta-Espinoza,Griselda; Valdez-Melara,Marta; Pereira,Luiz Filipe Protasio; Gatica-Arias,Andrés.
Abstract The influence of silver nitrate (AgNO3), benzyladenine (BAP), and indole-3-acetic acid (IAA) on low frequency somatic embryogenesis (LFSE) induction in Caturra and Catuaí arabica coffee was evaluated. For the Caturra cultivar, the production of somatic embryos was significantly increased by adding AgNO3 to the semisolid culture medium. The highest average number of somatic embryos for this cultivar was obtained using 6.6 μM BAP, 2.85 μM IAA, and 40 μM AgNO3. In contrast, for the Catuaí cultivar, the highest average number of somatic embryos was obtained using semisolid medium supplemented with 8.8 μM BAP, and 2.85 μM IAA. Using these protocols, somatic embryos were directly induced using leaf sections of in vitro plants of both coffee cultivars...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffee; Silver nitrate; Somatic embryos; Plant growth regulators; Tissue culture.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100408

Morpho and Cytological Differentiation of Calli of Eucalyptus grandis x Eucalyptus urophylla During Somatic Embryogenesis
Provedor de dados: 52
Autores: Batista,Tânia Regna; Mendonça,Evânia Galvão; Pádua,Marlúcia Souza; Stein,Vanessa Cristina; Paiva,Luciano.
ABSTRACT The aim of this study was to induce and analyze embryogenic calli from two types of explants (leaves and meristems) of the hybrid Eucalyptus grandis x Eucalyptus urophylla. Leaves and meristems of plants kept in a nursery were disinfected and inoculated in Petri dishes containing MS culture medium supplemented with different concentrations of the growth regulator dicamba (1.13, 4.52, and 9.04 µM) and without it. At 60 days of culturing, the calli were analyzed by scanning electron microscopy and at 90 days were evaluated by light microscopy in regard to the embryogenic characteristics of the cells. Different type of calli were induced in leaf explants, designated as Type I with light yellow coloring, Type II with dark yellow coloring, and Type III...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomical analyses; Dicamba; Eucalyptus; Somatic embryos; Ultrastructural analyses.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100430

2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L.
Provedor de dados: 65
Autores: Alves,Ivanilda dos Santos; Carmazini,Valéria Cristina Barbosa; Santos,Cosme Damião dos; Almeida,Julieta Andrea Silva de.
ABSTRACT:  This study evaluated the effect of different concentrations of 2-isopentenyladenine (2-iP) on the direct somatic embryogenesis capacity of the Mundo Novo cultivar of Coffea arabica. Leaf explants were cultivated with half the MS salt concentration and the addition of sucrose (20gL-1) and 2-iP (0; 2.5; 5; 7.5 and 10µM). The 2-iP doses of 7.5 and 10µM produced the greatest responses with respect to the percentage of explants with embryogenic structures and the size of the embryogenic structures. However, the greatest production of somatic embryos occurred on the explants treated with 10µM of 2-iP, followed by 7.5µM, whereas their production was absent or reduced with 0 and 5µM, respectively.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cultivar Mundo Novo; 2-iP; Somatic embryos; Embryogenic structures.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100403