Consulta: subjectFacets:"Species identification"
Registros recuperados: 19
Data/hora: 06/06/2024 16:59:15

Etiología, patrón espacio-temporal y análisis filogenético de Armillaria spp., en árboles de durazno [Prunus persica (L.) Batsch.] en el sur del Estado de México.
Provedor de dados: 32
Autores: Elías Román, Rubén Damián.
La pudrición de raíces por Armillaria es responsable de la mortalidad significativa de árboles de duraznos en plantaciones del Estado de México, México. En 15 huertas monitoreadas, el promedio en mortalidad de árboles fue 9.7, 15.3 y 20.3% y el área afectada fue 23.2, 24.7 y 28.3% durante 2009, 2010 y 2011, respectivamente. Los portainjertos empleados (‘Nemaguard’ y ‘Criollo de la Goleta’) en la región fueron susceptibles a la enfermedad. Las especies de Armillaria se identificaron mediante la secuenciación de dos regiones de ADN ribosomal (5.8S partial-ITS2-LSU D-dominios y 3' LSU parcial-IGS1) y el gen del factor de elongación-1α (tef-1), las secuencias de ADN se compararon con las especies de Armillaria en el GenBank y se realizaron análisis...
Palavras-chave: Filogenia; Identificación de especies; Portainjertos; Patogenicidad; Enfermedades de raíz; Phylogeny; Species identification; Rootstocks; Pathogenicity; Armillaria root disease; Doctorado; Fruticultura.
Ano: 2013
URL: http://hdl.handle.net/10521/1856

Electronic Field Guides and User Communities in the Eco-informatics Revolution
Provedor de dados: 7
Autores: Stevenson, R. D.; University of Massachusetts Boston; robert.stevenson@umb.edu; Haber, William A; Missouri Botanical Garden; whaber@racsa.co.cr; Morris, Robert; UMASS Boston; ram@cs.umb.edu.
The recognition that taxonomy is central to the conservation of biodiversity has reestablished the critical role of taxonomy in biology. However, many of the tools taxonomists produce for the identification and characterization of species, e.g., dichotomous keys, have been difficult to use and largely ignored by the general public in favor of field guides, which are essentially browsable picture guides. We review the role of field guides in species identification and discuss the application of a host of digital technologies to produce user-friendly tools for identification that are likely to greatly enhance species identification in the field by nonspecialists. We suggest that wider adoption of the citizen science model and the use of electronic field...
Tipo: Peer-Reviewed Reports
Palavras-chave: Bioinformatics; Birding; Citizen science; Ecoinformatics; Field biology; Field guides; Species identification; Taxonomic keys; Taxonomy.
Ano: 2003

Discrimination of Shark species by simple PCR of 5S rDNA repeats
Provedor de dados: 74
Autores: Pinhal,Danillo; Gadig,Otto BF; Wasko,Adriane P; Oliveira,Claudio; Ron,Ernesto; Foresti,Fausto; Martins,Cesar.
Sharks are suffering from intensive exploitation by worldwide fisheries leading to a severe decline in several populations in the last decades. The lack of biological data on a species-specific basis, associated with a k-strategist life history make it difficult to correctly manage and conserve these animals. The aim of the present study was to develop a DNA-based procedure to discriminate shark species by means of a rapid, low cost and easily applicable PCR analysis based on 5S rDNA repeat units amplification, in order to contribute conservation management of these animals. The generated agarose electrophoresis band patterns allowed to unequivocally distinguish eight shark species. The data showed for the first time that a simple PCR is able to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chondrichthyes; PCR; Species identification; 5S rDNA; Sharks.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200033

Variação geográfica no período de nidificação do jacaré-paguá, Paleosuchus palpebrosus, no Brasil.
Provedor de dados: 14
Autores: CAMPOS, Z.; SANAIOTTI, T.; MAGNUSSON, W.
O objetivo deste estudo é divulgar os avanços de conhecimento da biologia da reprodução de jacaré-paguá, Paleosuchus palpebrosus, nos diferentes ambientes da floresta alagada da Amazônia e nos riachos do entorno do Pantanal. Os resultados apresentados encontram-se alinhados ao Objetivo de Desenvolvimento Sustentável das Nações Unidas n° 15 - Vida Terrestre, no que diz respeito à sua Meta 15.1.- assegurar a conservação, recuperação e uso sustentável de ecossistemas terrestres e de água doce interiores e seus serviços, em especial florestas, zonas úmidas, montanhas e terras áridas, em conformidade com as obrigações decorrentes dos acordos internacionais (Nações Unidas Brasil, 2022).
Tipo: Folhetos
Palavras-chave: Selo ODS 15; Jacaré; Preservação da Natureza; Animal Selvagem; Caiman; Habitat preferences; Species identification.
Ano: 2022
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1148401

Genetic diversity, paraphyly and incomplete lineage sorting of mtDNA, ITS2 and microsatellite flanking region in closely related Heliopora species (Octocorallia)
Provedor de dados: 5
Autores: Yasuda, Nina; Taquet, Coralie; Nagai, Satoshi; Fortes, Miguel; Fan, Tung-yung; Harii, Saki; Yoshida, Terutoyo; Sito, Yuta; Nadaoka, Kazuo.
Examining genetic diversity and lineage sorting of different genes in closely related species provide useful information for phylogenetic analyses and ultimately for understanding the origins of biodiversity. In this study, we examined inter- and intraspecific genetic variation in internal transcribed spacer 2 (ITS2), partial mitochondrial gene (mtMutS), and nuclear microsatellite flanking region in two closely related octocoral species (Heliopora coerulea, HC-A and HC-B). These species were recently identified in a population genetic study using microsatellite markers. The two species have different reproductive timing, which ecologically promotes lineage sorting. In this study, we examined whether species boundaries could be detected by the commonly used...
Tipo: Text
Palavras-chave: ITS2; Concerted evolution; MtMutS; Secondary structure; Species identification; Speciation.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00667/77932/80144.pdf

Fishes from the hydrothermal vents and cold seeps - An update
Provedor de dados: 5
Autores: Biscoito, Manuel; Segonzac, Michel; Almeida, Armando; Desbruyeres, Daniel; Geistdoerfer, Patrick; Turnipseed, Mary; Van Dover, Cindy.
Introduction : Since the discovery of animal communities in oceanic hydrothermal vents in 1977 and in deep-sea cold seeps in 1984 (Londsdale, 1977; Paull et al., 1984) fishes have been regularly observed in association with these chemosynthetically-driven communities, but in most cases they are difficult to catch and therefore species identification can only rely on images taken by the diving vehicles. Ichthyological information pertaining to species inhabiting the deep-sea hydrothermal vents and cold seeps is mentioned in over 30 papers and even in the more detailed and updated lists (Geistdoerfer, 1991; 1996; 1999; Sibuet &amp; Olu, 1998; Tunnicliffe, 1991) there are species missing. The situation is even less clear concerning the bathyal species...
Tipo: Text
Palavras-chave: Fishes; Species identification; Hydrothermal vent.
Ano: 2002
URL: http://archimer.ifremer.fr/doc/2002/publication-896.pdf

Premieres remarques sur les peuplements de l'etage bathyal de la basse Adriatique
Provedor de dados: 5
Autores: Bombace, G; Froglia, C.
Exploratory trawl fishing was performed in the Southern Adriatic Sea. Comparison of bionomic characteristics and bottom fauna of Southern Adriatic with Southern Tyrrenian ones, led to the following observations: a bathymetric expansion of the bathyl biocoenosis horizons, absence of spp which are very abundant in Southern Tyrrenian bathyal biocoenosis, reduction of mean size for some spp, a general impoverishment of the bathyal biocoenosis itself, presence of new sp for the Adriatic sea.
Tipo: Text
Palavras-chave: South Adriatic; Species identification; Bathyal fauna; Trawled species; Biocenosis.
Ano: 1973
URL: http://archimer.ifremer.fr/doc/1973/publication-2158.pdf

Authenticating the origin of different shrimp products on the Tunisian markets by PCR/RFLP method
Provedor de dados: 5
Autores: Besbes, Nadia; Jerome, Marc; Berge, Jean-pascal; Sadok, Saloua.
This study describes a polymerase chain reaction using restriction fragment length polymorphism (PCR-RFLP) assay based on the 16S rRNA mitochondrial gene to identify commercial food products of wide range of Penaeidae and Pandalidae shrimp species commercialised in the Tunisian market. Phylogenetic analyses on 16S rRNA mitochondrial gene were used to study the relationships among the considered species. Penaeidae shrimp species was easily differentiated and confirmed by direct sequencing, showing a genetic distance of 0.34 with respect to Pandalidae species. A rapid and reliable PCR method using restriction fragment length polymorphism (RFLP) with three restriction enzymes (HpyCH4III/ MboI / AluI) was optimized for unambiguous differentiation of shrimp...
Tipo: Text
Palavras-chave: Shrimps; Species identification; 16S rRNA mtDNA; PCR-RFLP.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00303/41445/40637.pdf

Proposals for the collection of multifrequency acoustic data
Provedor de dados: 5
Autores: Korneliussen, R; Diner, Noel; Ona, E; Berger, Laurent; Fernandes, P.
Acoustic surveys are used to estimate the abundance and distribution of many fish species, and have been based traditionally on data collected at a single acoustic frequency. Although it has been known for some time that the use of additional frequencies can provide information on the nature of the acoustic target, the knowledge and technology required to combine the so-called "multifrequency data" in an appropriate manner has been limited. The use of several transducers of different frequencies is now common on board research vessels and fishing vessels, so multifrequency data are often collected. In order for these data to be combined appropriately, their physical and spatial characteristics from each frequency should be as similar as possible. We detail...
Tipo: Text
Palavras-chave: Species identification; Multifrequency acoustics; Data collection.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-5286.pdf

Development of a qPCR Method for the Identification and Quantification of Two Closely Related Tuna Species, Bigeye Tuna (     Thunnus obesus     ) and Yellowfin Tuna (     Thunnus albacares     ), in Canned Tuna
Provedor de dados: 5
Autores: Bojolly, Daline; Doyen, Perine; Le Fur, Bruno; Christaki, Urania; Verrez-bagnis, Veronique; Grard, Thierry.
Bigeye tuna (Thunnus obesus) and yellowfin tuna (Thunnus albacares) are among the most widely used tuna species for canning purposes. Not only substitution but also mixing of tuna species is prohibited by the European regulation for canned tuna products. However, as juveniles of bigeye and yellowfin tunas are very difficult to distinguish, unintentional substitutions may occur during the canning process. In this study, two mitochondrial markers from NADH dehydrogenase subunit 2 and cytochrome c oxidase subunit II genes were used to identify bigeye tuna and yellowfin tuna, respectively, utilizing TaqMan qPCR methodology. Two different qPCR-based methods were developed to quantify the percentage of flesh of each species used for can processing. The first one...
Tipo: Text
Palavras-chave: Tuna; Authentication; Quantification; Canned products; TaqMan; QPCR; Species identification; Bigeye tuna (Thunnus obesus); Yellowfin tuna (Thunnus albacares).
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00371/48217/48403.pdf

Current Methods for Seafood Authenticity Testing in Europe: is there a Need for Harmonisation?
Provedor de dados: 5
Autores: Griffiths, Andrew M.; Sotelo, Carmen G.; Mendes, Rogerio; Perez-martin, Ricardo I.; Schroeder, Ute; Shorten, Marc; Silva, Helena A.; Verrez-bagnis, Veronique; Mariani, Stefano.
Mislabelling of food products has recently received a great deal of public scrutiny, but it remains unclear exactly what methods are being utilised in laboratories testing the authenticity of foods. In order to gain insight into the specific area of the analysis of seafood, a questionnaire focusing on the taxonomic groups typically analysed and the techniques utilised was sent to over one hundred accredited laboratories across the UK, Ireland, Spain, Portugal, France and Germany. Forty-five responded positively, demonstrating significant differences in both the species analysed and methods utilised among the countries included in the survey. Indeed, a diversity of methods was employed across laboratories and efforts to harmonise and/or standardise testing...
Tipo: Text
Palavras-chave: Food testing; Forensically informative nucleotide sequencing; Species identification; DNA barcoding; Fisheries.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00188/29918/28383.pdf

Development of in situ hybridisation using 16S rRNA gene to monitor black-lip pearl oyster, Pinctada margaritifera, larvae in plankton samples
Provedor de dados: 5
Autores: Thomas, Yoann; Belliard, Corinne; Garen, Pierre; Gueguen, Yannick; Montagnani, Caroline.
In French Polynesia, the black-lip pearl oyster Pinctada margaritifera has been farmed to produce pearls since the 1980s, forming the basis of a major industry. The sustainability of this activity relies on spat collection in the lagoons. However, pearl oyster spat can be difficult to identify for the evaluation of stock variations. It is especially hard to distinguish Pinctada spp. larvae at a very early stage of development. In the present study, a whole- mount in situ hybridisation (ISH) technique was developed to allow the discrimination of larvae of closely-related pearl oyster species found in the French Polynesian atolls. Using specific ribosomal 16S-DNA sequence data, we were able to successfully differentiate between Pinctada margaritifera and...
Tipo: Text
Palavras-chave: Whole larvae in situ hybridisation; 16S rRNA; Plankton; Species identification; Pearl oyster; Pinctada margaritifera.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00035/14603/11923.pdf

A method for reducing uncertainty in estimates of fish-school frequency response using data from multifrequency and multibeam echosounders
Provedor de dados: 5
Autores: Berger, Laurent; Poncelet, Cyrille; Trenkel, Verena.
Fish schools can be insonified simultaneously with multifrequency echosounders (e.g. Simrad EK60s) and a multibeam echosounder (e.g. Simrad ME70). This paper presents a method for combining these data to improve estimates of the relative frequency response r(f) of fish schools. Values of r(f) are now commonly used to classify echoes in fishery surveys. The data from the roll- and pitch-stabilized, high-resolution ME70 are used to correct beam-width effects in the multifrequency EK60 data. First, knowing the exact position and orientation of the transducers and the position of the vessel, the echoes are placed into a common geographic coordinate system. Then, the EK60 data are rejected if they do not include a significant percentage of the fish school...
Tipo: Text
Palavras-chave: Species identification; Scomber scombrus; Sardina pilchardus; Pelagic fish; Acoustics.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6590.pdf

Neural network-based species identification in venom-interacted cases in India
Provedor de dados: 80
Autores: Maheshwari,R.; Kumar,V.; Verma,H. K.
India is home to a number of venomous species. Every year in harvesting season, a large number of productive citizens are envenomed by such species. For efficient medical management of the victims, identification of the aggressor species as well as assessment of the envenomation degree is necessary. Species identification is generally based on the visual description by the victim or a witness and is therefore quite likely to be erroneous. Symptomatic identification remains the only available method. In a previous published work, the authors proposed a classification table for snake species based on manifested symptoms applicable in Indian subcontinent. The classification table serves the purpose to a great deal but as a manual method it demands human...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bites and stings; Symptoms; Species identification; Neural network.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400008

Cytogenetical and morphological features reveal significant differences among Venezuelan and Brazilian samples of Mugil curema (Teleostei: Mugilidae)
Provedor de dados: 83
Autores: Nirchio,Mauro; Cipriano,Roger; Cestari,Margarete; Fenocchio,Alberto.
Karyotype of M. curema from the Gulf of Mexico and Brazil have been reported as possessing chromosome complement with 2n=28 and FN=48, whereas specimens from Venezuela has been reported as possessing a diploid number 2n=24 and a conserved FN (48). Although at first sight this variation suggests the presence of a chromosomal intraspecific (interpopulational) variability, the possibility that we are dealing with two different species was examined. This work revisit the karyotypes of M. curema from Venezuela and Brazil, including new data on C-banding, and NOR localization, and compares morphologic characteristics of samples from both localities. Thus, besides diploid number, the constitutive heterochromatin distribution and NORs location, mark other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Karyotype; C-band; NOR-banding; Meristic and morphometric features; Species identification.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252005000100006

Benefits from ecological study methods to taxonomy of enchytraeids in southern Mata Atlântica
Provedor de dados: 86
Autores: Schmelz,Rüdiger Maria; Peña,Rut Maria Collado de la; Römbke,Jörg.
The objective of this work was to determine how taxonomy benefited from the ecological quantitative and site-based sampling methods in enchytraeids studies. Enchytraeids (small relatives of earthworms) were sampled in different phases of rain forest regeneration in the southern Mata Atlântica in Paraná, Brazil. The research combined ecological and taxonomic work, because enchytraeids are poorly studied and difficult to identify, and many new species were expected. The provision of large numbers of specimens enabled the test of species diagnoses by investigating the ranges of character variations in a larger series of specimens. Simplified species diagnoses adapted to the local conditions that allowed the identification of all specimens, juveniles included,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clitellata; Enchytraeidae; Oligochaeta; South America; Species identification.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000800009

DNA barcodes for soil animal taxonomy
Provedor de dados: 86
Autores: Rougerie,Rodolphe; Decaëns,Thibaud; Deharveng,Louis; Porco,David; James,Sam W.; Chang,Chih-Han; Richard,Benoit; Potapov,Mikhail; Suhardjono,Yayuk; Hebert,Paul D.N.
The biodiversity of soil communities remains very poorly known and understood. Soil biological sciences are strongly affected by the taxonomic crisis, and most groups of animals in that biota suffer from a strong taxonomic impediment. The objective of this work was to investigate how DNA barcoding - a novel method using a microgenomic tag for species identification and discrimination - permits better evaluation of the taxonomy of soil biota. A total of 1,152 barcode sequences were analyzed for two major groups of animals, collembolans and earthworms, which presented broad taxonomic and geographic sampling. Besides strongly reflecting the taxonomic impediment for both groups, with a large number of species-level divergent lineages remaining unnamed so far,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Collembola; Cryptic diversity; DNA barcoding; Oligochaeta; Species identification; Taxonomic crisis; Taxonomic impediment.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000800002

Total protein electrophoresis and RAPD fingerprinting analysis for the identification of Aeromonas at the species level
Provedor de dados: 58
Autores: Delamare,Ana Paula Longaray; Artico,Liane de Oliveira; Grazziotin,Felipe Gobbi; Echeverrigaray,Sergio; Costa,Sérgio Olavo Pinto da.
Fifteen well-defined strains of Aeromonas of thirteen species were analyzed by SDS protein electrophoretic analysis (SDS-PAGE) and random amplified polymorphic DNA analysis (RAPD). The comparison between the patterns obtained by both methods allowed differentiating all the strains. Clusters formed by the unweighted pair group method with arithmetic averages applied to protein data correlates with the genetic and biochemical information about the species. The results show that protein fingerprinting has the potential to differentiate Aeromonas species, but the low qualitative variation indicates that this technique is not efficient for the characterization of strains within a species. Conversely, RAPD fingerprinting allows the identification of strains but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aeromonas; Molecular markers; Species identification.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400016

Brazilian ground beef authentication by multiplex polymerase chain reaction
Provedor de dados: 65
Autores: Oliveira,Andrey Carlos do Sacramento de; Pedroso,Silvia Cristina da Silva; Cardilli,Diogo José; Leite,Fábio Pereira Leivas; Ferreira,Gabrielle Virgínia Lopes; Silva,Andréia Silva da; Roos,Talita Bandeira; Moraes,Carina Martins de; Sousa,Roberta Sales; Monteiro,Roseli do Socorro Dias.
ABSTRACT: The aim of the present study was to assess the efficacy ofmultiplex PCR in detecting the adulterationof commercially available ground beefvia addition and/orsubstitution ofground buffalo meat. Experimentally adulterated ground beefsamples were prepared in triplicate, and dilutions of DNA from Bos taurus and Bubalusbubalis were prepared to determine the detection limit of the method. Concurrently, 91 ground meatsamples sold as “ground beef” were collected from differentstores in northern Brazil andanalyzed bymultiplex PCR. Buffalo DNA was detected in 17.5% of the collected ground meat samples.Our results showed that multiplex PCR is an efficient method for detectingthe incorporation of groundbuffalo meatatpercentages ranging from 10 to 100% and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Species identification; Bos taurus; Bubalusbubalis; DNA; Adulteration.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200652