Consulta: subjectFacets:"Spodoptera litura"
Registros recuperados: 2
Data/hora: 01/06/2024 13:44:44

Recombinant expression and refolding of the c-type lysozyme from Spodoptera litura in E. coli
Provedor de dados: 69
Autores: Kim,Jong-Wan; Yoe,Jeehyun; Lee,Gil Ho; Yoe,Sung Moon.
The chicken-type lysozyme of the insect Spodoptera litura (SLLyz) is a polypeptide of 121 amino acids containing four disulfide bridges and 17 rare codons and participates in innate defense as an anti-bacterial enzyme. The recombinant S. litura lysozyme (rSLLyz) expressed as a C-terminal fusion protein with glutathione S-transferase (GST) in Rosetta(DE3) Singles. The protein was produced as an inclusion body which was solubilized in 8 M urea, renatured by on-column refolding, and purified by reversed-phase chromatography to 95% purity. The purified rSLLyz demonstrated antibacterial activity against B. megaterium confirmed by inhibition zone assay. The overexpression and refolding strategy described in this study will provide a reliable technique for...
Tipo: Journal article
Palavras-chave: Antibacterial activity; Inclusion body; Lysozyme; On-column refolding; Recombinant expression; Spodoptera litura.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300006

Effect of PONNEEM# on Spodoptera litura (Fab.) and its compatibility with Trichogramma chilonis Ishii
Provedor de dados: 52
Autores: Packiam,Soosaimanickam Maria; Ignacimuthu,Savarimuthu.
Antifeedant and growth regulating activities of PONNEEM, an oil formulation containing neem and pungam (karanj) oils were evaluated along with individual neem and karanj oils and Nimbicidine, a commercial neem-based pesticide against fourth instar larvae of Spodoptera litura (Fab.). Among all the treatments, PONNEEM recorded the maximum antifeedant activity (88.6%) at 0.6%. Neem and karanj individual treatments as well as PONNEEM extended larval duration compared to control. Pupal weight and fecundity were significantly reduced and pupal period was greatly increased by PONNEEM treatment compared to other treatments. The PONNEEM was found to be compatible with Trichogramma chilonis Ishii, an egg parasitoid of many lepidopteran pests, at 0.15, 0.3 and 0.5%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oil formulation; Spodoptera litura; Antifeedant activity; Growth regulating activity; Trichogramma chilonis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200016