Consulta: subjectFacets:"Stereology"
Registros recuperados: 31
Data/hora: 14/06/2024 20:08:03

Comparison of dietary phospholipids and neutral lipids: effects on gut, liver and pancreas histology in Atlantic cod (Gadus morha L.) larvae
Provedor de dados: 5
Autores: Wold, P. -a.; Hoehne-reitan, K.; Cahu, Chantal; Zambonino, Jose-luis; Rainuzzo, J.; Kjorsvik, E.
The aim of the present study was to compare effects of dietary n-3 highly unsaturated fatty acids (HUFA) being incorporated in the phospholipid (PL) or in the neutral lipid (NL) fraction of the larval feed, on larval growth and histology of digestive organs in Atlantic cod (Gadus morhua L.) larvae. Three isoproteic and isolipidic diets, labelled according to the percentage of n-3 docosahexaenoic acid and eicosapentaenoic acid contained in NL1 or in PL1 and PL3 of the diets, were fed to cod larvae from 17 days post hatching (dph) to 45 dph. In the liver, hepatocytes and their nuclei were smaller in NL1 larvae compared with the PL larvae; the mitochondrial membrane structures were less dense and the amount of lipids observed in the liver was significantly...
Tipo: Text
Palavras-chave: Stereology; Phospholipids; Nutrition; Lipid class; Larvae; Histology; Diet; Cod.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6777.pdf

Gametogenesis, reproductive investment, and spawning behavior of the Pacific giant oyster Crassostrea gigas: evidence of an environment-dependent strategy
Provedor de dados: 5
Autores: Enriquez-diaz, M.; Pouvreau, Stephane; Chavez-villalba, J.; Le Pennec, M.
The progress of gametogenesis was studied in oysters Crassostrea gigas having the same origin (Tremblade), but cultured during 1 year in two distinctive French marine areas, the Baie des Veys and Marennes-Ol,ron. We assessed seasonal changes in the reproduction cycle on the basis of stereological techniques to estimate reproductive investment and measurement of gonad evolution area by quantitative histology. From a qualitative point of view, both oyster groups presented typical reproductive stages, but showed differential timing, in particular during the sequence of spawning and duration of the re-absorption stage. Oysters in Baie des Veys had a single partial spawning in August and a re-absorption stage that extended until winter. Oysters in...
Tipo: Text
Palavras-chave: Stereology; Reproductive investment; Pacific oysters; Image analysis; Gametogenesis; Crassostrea gigas.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-7300.pdf

Effects of Preweaning Polysensorial Enrichment upon Development of the Parietal Cortical Plate of Undernourished Rats: A Stereological Study
Provedor de dados: 77
Autores: González,Héctor; Adaro,Luis; Hernández,Alejandro; Fernández,Víctor.
This investigation was undertaken in order to quantify the effects of early polysensorial enrichment on the development of cortical pyramids, located in the parietal cortex of rats simultaneously submitted to protein-energy undernutrition. A short period of stimulation during suckling significantly decreases the cellular density in the cortical plate (phylogenetic-ontogenetic evolutionary index). Results suggest that the cerebral cortex develops according to a sophisticated neuronal network, which exhibits a notable degree of structural specificity, regulated by genetic and environmental clues. The most obvious prediction is that environmental influences are probably transduced as a structural expression in the developing parietal cortical plate.
Tipo: Journal article
Palavras-chave: Environmental enrichment; Cortical gradients; Undernutrition; Parietal cortex; Stereology.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400016

EFFECT OF CADMIUM ON RAT MAXILLARY MOLAR JUNCTIONAL EPITHELIUM DURING LACTATION: EFECTO DEL CADMIO SOBRE EL EPITELIO DE LA ZONA DE UNIÓN MAXILO-MOLAR DE RATAS DURANTE LA LACTANCIA
Provedor de dados: 77
Autores: Ribas,Paulo; Lopes,Ruberval A.; Sala,Miguel A.; Ribas,Lucia M.R.; de Mattos,Maria da Glória C.; Semprini,Marisa; Watanabe,Ii-Sei; Regalo,Simone C.H.
Cadmium in the air, drinking water and food has the potential to affect the health of people, mainly those who live in highly industrialized regions. Cadmium affects placental functions, can cross the placental barrier and directly disturbs fetal development. It is also excreted into milk. The organism is particularly susceptible to cadmium exposure at perinatal period. The effect on rat maxillary molar junctional epithelium a continuous exposure to drinking water containing low level of cadmium during lactation was studied. Female rats received drinking water ad libitum containing 300 mg/l CdCl2 throughout the whole lactation. Control animals received a similar volume of water without cadmium. Lactent rats (21 day-old) were killed by lethal dose of...
Tipo: Journal article
Palavras-chave: Oral mucosae; Junctional epithelium; Cadmium; Lactation; Morphometry; Stereology.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000400003

Determination of Lateral Ventricle and Brain Volume in Children with Stereological Method Using MRI
Provedor de dados: 77
Autores: Karacan,Keziban; Kosar,Mehmet Ilkay; Çimen,Mehmet; Solak,Orhan; Sahin,Bünyamin.
Although brain development is most active during the intrauterina period of life, the processes of myelination and arborization affect the structure of the brain throughout childhood and adolescence. Brain development is also very active in the early years of a child's life, and continues to be so for approximately 15 years after gestation. Volumetric changes in the brain are effected by sex. Understanding the variability of human brain volume during development is important for the interpretation of childhood neuroimaging studies. Hence the aim of this study is to determine the effects of sex difference on brain volume (BV), lateral ventricle volume (LV) and the ratio of brain volume and lateral ventricle volumes as a percentage (RLBV%) of 90 healthy...
Tipo: Journal article
Palavras-chave: Brain; Lateral ventricles; Magnetic resonance imaging; Stereology.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100035

High Concentration of Reticular Fibers in Periurethral Region of the Prostatic Urethra from Patients with Benign Prostatic Hyperplasia as Revealed by Stereology
Provedor de dados: 77
Autores: Manaia,Jorge H. M; Costa,Waldemar S; Sampaio,Francisco J. B; Figueiredo,Marcelo A; Cardoso,Gilberto P; Babinski,Marcio A.
The goal of this investigation was to analyze and quantify changes of the reticular fibers in the prostatic urethra of patients with benign prostatic hyperplasia (BPH) and compare with a control group. Prostatic urethra tissue samples were obtained from ten patients (age range 65 to 79 years, mean 66) with clinical symptoms of bladder outlet obstruction who had undergone open prostatectomy. The ten control group samples (urethral tissue samples from the transitional zone) were collected from prostates obtained during autopsy of accidental death adults of less than 25 years. The Vv of the reticular fibers was determined with stereologic methods from 25 random fields per sample using the point-count method with a M-42 grid test system. The quantitative data...
Tipo: Journal article
Palavras-chave: Prostatic urethra; Reticular fibers; Stereology.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300065

Effect of Sodium Cyclamate on the Rat Fetal Exocrine Pancreas: a Karyometric and Stereological Study
Provedor de dados: 77
Autores: Martins,Alex Tadeu; Santos,Fabiano de Sant´Ana dos; Scannavino,Fábio Luiz Ferreira; Pires,Juliana Rico; Zuza,Elizângela Partata; Junior,João Armando Padovani; Azoubel,Reinaldo; Di Mateo,Miguel Angel Sala; Lopes,Ruberval Armando.
The cyclamate, a sweetner substance derived from N-cyclo-hexyl-sulfamic acid, is largely utilized as a non-caloric artificial edulcorant in foods and beverages as well as in the pharmaceutical industry. The objective of this study was to evaluate karyometric and stereological alterations in the rat fetal pancreas resulting from the intraperitoneal administration of sodium cyclamate. The exocrine pancreas of ten fetuses of rats were evaluated, five treated and five controls chosen at random, in which five rats that received from the 10th to 14th days of pregnancy an intraperitoneal daily injection of sodium cyclamate at 60 mg/Kg of body weight during 5 days. At the 20th day of gestation, the animals were removed and weighed, as were their placentas; the...
Tipo: Journal article
Palavras-chave: Sodium cyclamate; Exocrine pancreas; Karyometry; Stereology.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300038

MORPHOMETRICAL AND STEREOLOGICAL ANALYSIS OF THE SUPERIOR CERVICAL GANGLION OF Rattus norvegicus SUBMITTED TO CHRONIC TREATMENT WITH CORTISOL
Provedor de dados: 77
Autores: Cortez,C. M.; Costa,W. S.; Babinski,M. A.; Chagas,M. A.
The present work investigates the corticoid action on the growth of the superior cervical ganglion of the rat and describes the cortisol effect during early stages of development. The study was based on morphometric and stereological analysis of the perikarya. Eight rats were treated intraperitoneally with cortisol (1mg/Kg/day) during 36 days. Treatment was initiated in the 8th day after birth and was withdrawn one day before the sacrifice. There was a significant difference (&lt;=P0,05) for the neural mean diameter between the control group (16.78 ± 1.11mm) and treated animals (15.84 ±0.99mm). The decrease of perikarya neuronal diameter was also demonstrated by stereological methods. Morphometrical findings may suggest alterations in superior cervical...
Tipo: Journal article
Palavras-chave: Morphometry; Stereology; Glucocorticoid; Sympathetic ganglion; Rat.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000300006

Morphometric Analysis of Hemicerebellar Asymmetryn with Central Vertigo Cases: A Stereological Study
Provedor de dados: 77
Autores: Nuket Gocmen-Mas,H; Karabekir,Selim; Yilmaz Kusbeci,Ozge; Sahin,Bunyamin; Ertekin,Tolga; Bas,Orhan; Canan Yazici,A; Senan,Sevda.
We aimed to evaluate the relevant methods of stereology to estimate hemicerebellar asymmetry according to sex in both adult right handed vertigo cases and comparing with healthy cases. The study included 14 adult control subjects and 18 patients with vertigo. The volumes of the cerebellar hemispheres were determined by MRI using the point-counting approach of stereological methods. The mean (±SD) of the right cerebellar hemispheres in the patients with vertigo were 52.49±5.42 cm3 in males, 50.11±4.02 cm3 in females. The mean (±SD) of the left cerebellar hemispheres in the patients with vertigo were 53.11±3.70 cm3 in males, 49.73±4.69 cm3 in females. There was not significant quantitative evidence detected in terms of cerebella asymmetry between sagittal...
Tipo: Journal article
Palavras-chave: Cavalier principle; Stereology; Hemicerebellar asymmetry; Magnetic resonance imaging; Vertigo.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200048

Effect of Photoperiod and Melatonin on Volume and Cellular Parameters of Testis in Pinealectomized Rats
Provedor de dados: 77
Autores: Akosman,Murat Sirri; Özdemir,Vural; Taskiran,Nihal; Akalan,Mehmet Aydin; Bülbül,Aziz.
Mammalian reproductive axis is regulated by the combination of three fundamental tissues of neuroendocrine system including hypothalamus, hypophysis and gonads. In recent years, pineal gland has been included in this axis. The aim of the present study was to investigate the effect of 12L (Light):12D (Dark) photoperiod and melatonin administration (0.5 mg/kg/day; subcutaneously) on testicular volume and cellular parameters of testis at the pinealectomized (PE) rats. For this aim, twelve adult rats were firstly pinealectomized and then divided into two groups as GI and GII randomly. The GI rats served as control group and received only normal saline, whereas GII rats were the melatonin administered group. It was found that the total testicular volume,...
Tipo: Journal article
Palavras-chave: Cavalieri Method; Melatonin; Optical Fractionator; Pinealectomy; Stereology; Testis.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300046

ENALAPRIL AND VERAPAMIL ATTENUATE THE AORTIC WALL REMODELING IN NITRIC OXIDE DEFICIENCY
Provedor de dados: 77
Autores: Meirelles Pereira,Leila Maria; Gonçalves Bezerra,Daniele; Mandarim-de-Lacerda,Carlos Alberto.
Forty mature male rats (Wistar strain) were maintained alive for 40 days, separated in four groups of five rats each (Control, L-NAME, L-NAME + Enalapril, L-NAME + Verapamil). Three aortic wall parameters were analyzed, as follows: the Q A(SMN) (the number of the tunica media smooth muscle nuclear profiles per area), the S V(lamellae) (surface density of the aortic lamellae), and the aortic wall thickness (AWT). Rats of the L-NAME group showed high blood pressure and cardiac hypertrophy. The AWT, the Q A(SMN) and the S V(lamellae) showed the same tendency among the groups. The increased AWT, or the reduced Q A(SMN) and the S V(lamellae) in l-NAME rats were not completely prevented by the administration of enalapril or verapamil. Hypertension causes the AWT...
Tipo: Journal article
Palavras-chave: Nitric oxide; Aorta; Enalapril; Verapamil; Stereology.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000100002

Effect of Sodium Cyclamate on the Rat Fetal Liver: A Karyometric and Stereological Study
Provedor de dados: 77
Autores: Martins,Alex Tadeu; Azoubel,Reinaldo; Lopes,Ruberval A.; di Matteo,Miguel A. Sala; de Arruda,José Germano Ferraz.
The cyclamate, a sweetner substance derived from N-cyclo-hexyl-sulfamic acid, is largely utilized as a non-caloric artificial edulcorant in foods and beverages as well as in the pharmaceutical industry. The objective of this study was to evaluate fetal hepatic karyometric and stereological alterations in the rat fetal liver resulting from the intraperitoneal administration of sodium cyclamate. The livers of ten rats were evaluated, five treated and five controls chosen at random, in which five rats that received from the 10th to 14th days of pregnancy an intraperitoneal daily injection of sodium cyclamate at 60 mg/Kg of body weight during 5 days. At the 20th day of gestation, the animals were removed and weighed, as were their placentas, on a precision...
Tipo: Journal article
Palavras-chave: Sodium cyclamate; Fetal liver; Karyometry; Stereology.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000300005

Effects of Oral Nicotine on Rat Liver Stereology
Provedor de dados: 77
Autores: Valenca,Samuel Santos; Gouveia,Lucas; Alves Pimenta,Wagner; Porto,Luís Cristóvão.
Nicotine is the more abundant component in cigarette smoke. Because nicotine is first metabolized in the liver, our aim was to investígate the effects of nicotine on this organ by biochemical and stereological methods. Male Wistar rats were treated with oral nicotine (ON) diluted in drinking water during 32 days. The control group was treated with drinking water in the same period. Rats were sacrificed 24 hours after last day, the blood was collected and the liver was removed. Lipidogram was performed by enzymatic method and collagen fibers, fat globules and hepatocytes were count in the liver by stereological methods. We observed in control group preserved hepatocytes, with no presence of inflammatory cells. However in the ON group was possible to observe...
Tipo: Journal article
Palavras-chave: Nicotine; Rat; Liver; Stereology.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400037

End-To-Side Loop Neurorrhaphy: Axonal Comparative Stereological Study
Provedor de dados: 77
Autores: Boer,Nagib Pezati; Viterbo,Fausto; Faleiros,Humberto Regis; Batigalia,Fernando; Boer,Ana Luísa Ricci; Carvalho Jr,Altair Antonio.
Accidents or diseases can affect the peripheral part of the nervous system, which raises clinical and surgical therapies, among others. In this context, the technique of end-to-side neurorrhaphy is a treatment option, yet its modification loop needs some additional efficacy studies. The purpose of this study was to compare, among rats, stereological results (axons volume density) after end-to-side neurorrhaphy and after end-to-side loop neurorrhaphy. Thirty Wistar rats were used, divided into six groups (five animals per group), consisting of two control groups (for the fibular and tibial nerves), two study groups for the fibular nerve (one with an end-to-side neurorrhaphy, and the other with an end-to-side loop neurorrhaphy) and two study groups for the...
Tipo: Journal article
Palavras-chave: Nerve; Axon; Stereology; Neurorrhaphy.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000300032

Beneficial Effects of Angiotensin II AT1 Blocker on Cardiovascular Adverse Remodeling Due to Nitric Oxide Synthesis Blockade
Provedor de dados: 77
Autores: Fernandes-Santos,Caroline; Mendonça,Leonardo de Souza; Mandarim-de-Lacerda,Carlos Alberto.
We studied with morphological tools the effects of different doses of Losartan upon the cardiovascular remodeling in nitric oxide deficient rats. At 15 weeks of age, thirty Wistar rats were separated in six groups: control (C), L-NAME (LN), and four groups were LN was given plus Losartan at different doses (1, 5, 20 and 40 mg/kg/day). The L-NAME was given for 9 weeks, the Losartan administration starting on the 2nd week of experiment. We studied the heart, thoracic aorta and superior mesenteric artery with light microscopy and stereology. The blood pressure (BP) increased since the first week of L-NAME administration, the Losartan treatment at doses of 20 and 40 mg/kg/day was efficient to reduce BP after the 7th week of treatment. The cardiac adverse...
Tipo: Journal article
Palavras-chave: Losartan; Angiotensin II; L-NAME; Hypertension; Renin-angiotensin system; Stereology; L-NAME.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000400003

Evaluation of Lumbar Vertebral Body and Disc: A Stereological Morphometric Study
Provedor de dados: 77
Autores: Gocmen-Mas,Nuket; Karabekir,Hamit; Ertekin,Tolga; Edizer,Mete; Canan,Yazici; Duyar,Izzet.
Anterior access to the L1-L5 vertebrae and disc spaces can be technically challenging, frequently requiring the use of an approach to a surgeon for an adequate exposure. The technique is used for lesion excision, corpectomy, vertebral body reconstruction with cages, realignment, and/or plating or screwing. For a successful anterior approach and a suitable instrumental design via screw, adequate morphometric knowledge about body of lumbar vertebrae and disc spaces and standardized volumetric data are also required for neurosurgeons. We aimed morphometric and volumetric evaluation of lumbar bodies and discs to contribute to a safe anterior approach during surgery. We evaluated vertebral body and disc morphometry using stereology in right-handed 25 adult...
Tipo: Journal article
Palavras-chave: Lumbar vertebra; Body; Morphometry; Volumetry; Stereology; Anterior access.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300028

Which Method is Gold Standard for Determination of Thyroid Volume?
Provedor de dados: 77
Autores: Erçikti,Nurcan; Acer,Niyazi; Apaydi,Nihal; Güven,Inanç; Zararsiz,Gökmen.
Change of the thyroid gland volume is often the symptom of most common pathological conditions some thyroid diseases. The exact calculation for the thyroid volume is very important for the assessment and management of thyroid disorders. The volume of thyroid gland, using computed tomography (CT), ultrasound (USG) and magnetic resonance imaging (MRI) has been accessed in few studies published; however a gold standard method has not yet been determined. The purpose of this study was to estimate the volume of normal thyroid gland to define an optimal correction factor therefore was to compare different techniques using the CT. We used computed tomography images obtained from 8 cadavers (2 females, 6 males) to calculate the thyroid volumes. In the present...
Tipo: Journal article
Palavras-chave: Thyroid volume; Actual volume; Correction factor; Stereology.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200011

High-Intensity Interval Training Beneficial Effects in Diet-Induced Obesity in Mice: Adipose Tissue, Liver Structure, and Pancreatic Islets
Provedor de dados: 77
Autores: Motta,Victor F; Aguila,Marcia B; Mandarim-de-Lacerda,Carlos A.
The study aimed to evaluate the impact of high-intensity interval training (HIIT) on adipose tissue, pancreatic islets and liver in mice fed high-fat diet. C57BL/6 male mice were fed one of two diets: standard chow (Lean group - LE) or a high-fat diet (Obese group ­ OB). After the first 12-weeks, the animals were divided into non-trained (LE-NT and OB-NT), trained groups (LE-T and OB-T), and started the exercise protocol. The HIIT protocol in the trained animals (LE-T and OB-T) compared to their counterparts (LE-NT and OB-NT) led to a reduction in size of the pancreatic islets (LE-T vs. LE-NT -40 %, OB-T vs. OB-NT -22 %) and to an increase in insulin immunodensity in pancreatic islet (LE-T vs. LE-NT +35 %, OB-T vs. OB-NT +31 %). Apart from the above...
Tipo: Journal article
Palavras-chave: HIIT; Obesity; Insulin Resistance; Hepatic Steatosis; Stereology.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200042

Kidney anatomy, histology and histometric traits associated to renosomatic index in Gymnotus inaequilabiatus (Gymnotiformes: Gymnotidae)
Provedor de dados: 83
Autores: Fernandes,Carlos Eurico; Marcondes,Sandrieley Fernanda; Galindo,Gizela Melina; Franco-Belussi,Lilian.
ABSTRACT The Gymnotus inaequilabiatus is a Neotropical fish widely distributed in marginal areas of bays. The aim of this study was to describe the main histological and histopathological traits in the head and exocrine kidney. Here, histometric and structural density techniques were associated with renosomatic index (RSI). The kidney was processed for light microscopy. Lipofuscin and hemosiderin content were visually estimated in the melonomacrophages centers (MMCs). All the biometric body variables were correlated with RSI, especially the kidney weight and gross lesions count. The general architecture of head and exocrine kidney was similar to that described for other teleost species. MMCs were prevalent in both portions and correlated with RSI in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Pathology; Renal morphology; Stereology; Teleost.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000500207

The impact of long-term consumption of diets enriched with olive, cottonseed or sesame oils on kidney morphology: A stereological study
Provedor de dados: 42
Autores: HASSANZADEH-TAHERI,MOHAMMADMEHDI; HASSANZADEH-TAHERI,MAHSA; JAHANI,FARNAZ; ERFANIAN,ZAHRA; MOODI,HESAM; HOSSEINI,MEHRAN.
Abstract: To date, most of studies have only focused on metabolic effects of dietary oils while recent evidence proposes that they can influence kidneys structure. Therefore, the aim of this study was to evaluate the impact of long-term consumption of olive, cottonseed and sesame oils on renal morphology in rats. 70 male Wistar rats randomly assigned into seven equal groups and treated with standard diet (control), the standard diet enriched with 10% or 20% (W/W) of either olive oil (OLI10%, OLI20%), cottonseed oil (COT10%, COT20%) or sesame oil (SES10%, SES20%) for 5 months. Quantitative features of the kidney including kidney and cortex volumes and the number of glomeruli were analyzed stereologically. Moreover, kidney sections histologically were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cottonseed oil; Histopathology; Kidney; Olive oil; Sesame oil; Stereology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300702

The quantification of testicular cells during the postnatal development in two Caviomorph rodents: the guinea pig (Cavia porcellus) and the cutia (Dasyprocta agouti)
Provedor de dados: 42
Autores: SIMÕES,LUCIANA S.; SASAHARA,TAIS H.C.; FAVARON,PHELIPE O.; MARQUES JÚNIOR,RICARDO; OLIVEIRA,MOACIR F. DE; MACHADO,MÁRCIA R.F.; MIGLINO,MARIA ANGELICA.
ABSTRACT The germinative, Sertoli and Leydig cells of two caviomorph rodents (Cavia porcellus and Dasyprocta agouti) were counted as well as the estimation of the total volume of the testis and the total volume of seminiferous tubules and interstitium in prepubertal, pubertal and adult animals. The number of spermatogonia, spermatocytes and spermatids cells increased during the pubertal phase in both rodents, notably the spermatid cells. The spermatocyte and spermatid slightly decreased in the adult of both rodents, but the increment in spermatogonia cells number was seen, mainly in cutias. The number of Sertoli cells increased in pubertal rodents, but in the adult the number reduced. Substantial number of Leydig cells was counted in pubertal and adult...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Germinative cells; Hystricomorpha; Reproduction; Stereology.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401745

Disector Z-axis mechanical method for stereology
Provedor de dados: 42
Autores: Xavier-Vidal,Ricardo.
The purpose of this work is to calibrate a not expensive microscope to be applied in Optical Disector estimation. The evaluation of "Z-axis" bias and the "Z-axis" calibration were made utilizing a cover slip and a "manual digimatic outside micrometer scale" (Mitutoyo, Japan). Calibrating the cover slip we performed the calibration of the "Z-axis" of a microscope. In the cover slip two lines were painted with different colors using a pen glass. A blue line was painted on the up surface and another line (red) on the bottom surface of the cover slip forming a cross. Two metal rings with one palette welded in each were adapted in the microscope. Other palette was welded in the gross focuses in order to restrict the route of the fine focuses (Z-axis study)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stereology; Disector; Optical disector; Morphometry; Z-axis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000200028

Stereological tools in biomedical research
Provedor de dados: 42
Autores: Mandarim-de-Lacerda,Carlos A.
Stereological studies are more and more frequent in literature, particularly in the development/evolution, pathology, and neurosciences areas. The stereology challenge is to understand the structural inner threedimensional arrangement based on the analysis of the structure slices only showing two-dimensional information. Cavalieri and Scherle's methods to estimate volume, and Buffon's needle problem, are commented in the stereological context. A group of actions is needed to appropriately quantify morphological structures (unbiased and reproducibly), e.g. sampling, isotropic and uniform randomly sections (Delesse's principle), and updated stereological tools (disector, fractionator, nucleator, etc). Through the correct stereology use, a quantitative study...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphometry; Stereology; Volume density; Surface density; Length density; Disector; Fractionator.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652003000400006

Long lasting structural changes in primary motor cortex after motor skill learning: a behavioural and stereological study
Provedor de dados: 48
Autores: MORALES,PAOLA.
Many motor skills, once acquired, are stored over a long time period, probably sustained by permanent neuronal changes. Thus, in this paper we have investigated with quantitative stereology the generation and persistence of neuronal density changes in primary motor cortex (MI) following motor skill learning (skilled reaching task). Rats were trained a lateralised reaching task during an "early" (22-31 days oíd) or "late" (362-371 days oíd) postnatal period. The trained and corresponding control rats were sacrificed at day 372, immediately after the behavioural testing. The "early" trained group preserved the learned skilled reaching task when tested at day 372, without requiring any additional training. The "late" trained group showed a similar capacity to...
Tipo: Journal article
Palavras-chave: Motor cortex; Plasticity; Persistence of structural changes; Aging; Motor skill learning (skilled reaching task); Stereology; Rat.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000400005

Testicular morphology of adult wistar rats treated with Rudgea viburnoides (Cham.) Benth. leaf infusion
Provedor de dados: 52
Autores: Monteiro,Juliana Castro; Matta,Sérgio Luis Pinto da; Predes,Fabrícia Souza; Paula,Tarcízio Antônio Rego de.
The present study was carried out to investigate the effects of Rudgea viburnoides infusion on the body biometry and testicular morphometry and stereology of adult Wistar rats. Two groups received the infusion daily at the concentration of 3 or 6 mg/mL for 40 days. The control group received only water. Neither the biometrical parameters nor the tubular diameter and the height of the seminiferous epithelium showed any significant alterations in the treated animals. Leydig cells stereology did not show any significant alterations in the treated animals. These results indicated that R. viburnoides did not cause alterations in body biometry and testicular morphometry.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plant; Congonha-de-bugre; Morphometry; Stereology; Leydig cell.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100013

Beneficial effects of exercise training (treadmill) on insulin resistance and nonalcoholic fatty liver disease in high-fat fed C57BL/6 mice
Provedor de dados: 56
Autores: Marques,C.M.M.; Motta,V.F.; Torres,T.S.; Aguila,M.B.; Mandarim-de-Lacerda,C.A.
C57BL/6 mice develop signs and symptoms comparable, in part, to the human metabolic syndrome. The objective of the present study was to evaluate the effects of exercise training on carbohydrate metabolism, lipid profile, visceral adiposity, pancreatic islet alterations, and nonalcoholic fatty liver disease in C57BL/6 mice. Animals were fed one of two diets during an 8-week period: standard (SC, N = 12) or very high-fat (HF, N = 24) chow. An exercise training protocol (treadmill) was then established and mice were divided into SC and HF sedentary (SC-Sed, HF-Sed), exercised groups (SC-Ex, HF-Ex), or switched from HF to SC (HF/SC-Sed and HF/SC-Ex). HF/HF-Sed mice had the greatest body mass (65% more than SC/SC-Sed; P &lt; 0.0001), and exercise reduced it by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Obesity; Lipid metabolism; Metabolic syndrome; Aerobic training; Fatty liver; Stereology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500008

Effect of praziquantel administration on hepatic stereology of mice infected with Schistosoma mansoni and fed a low-protein diet
Provedor de dados: 56
Autores: Barros,L.A.; Costa-Silva,M.; Biolchini,C.L.; Neves,R.H.; Machado-Silva,J.R.
A study was undertaken to investigate the effect of administering praziquantel (PZQ), focusing on the liver stereological findings of malnourished mice infected with Schistosoma mansoni. Thirty female Swiss Webster mice (age: 21 days; weight: 8-14 g) were fed either a low-protein diet (8%) or standard chow (22% protein) for 15 days. Five mice in each group were infected with 50 cercariae each of the BH strain (Brazil). PZQ therapy (80 mg/kg body weight, per day) was started on the 50th day of infection and consisted of daily administration for 5 days. Volume density (hepatocytes, sinusoids and hepatic fibrosis) was determined by stereology using a light microscope. Body weight gain and total serum albumin levels were always lower in undernourished mice....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Schistosoma mansoni; Low-protein diet; Stereology; Praziquantel.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900007

Microglia and neurons in the hippocampus of migratory sandpipers
Provedor de dados: 56
Autores: Diniz,C.G.; Magalhães,N.G.M.; Sousa,A.A.; Santos Filho,C.; Diniz,D.G.; Lima,C.M.; Oliveira,M.A.; Paulo,D.C.; Pereira,P.D.C.; Sherry,D.F.; Picanço-Diniz,C.W.
The semipalmated sandpiper Calidris pusilla and the spotted sandpiper Actitis macularia are long- and short-distance migrants, respectively. C. pusilla breeds in the sub-arctic and mid-arctic tundra of Canada and Alaska and winters on the north and east coasts of South America. A. macularia breeds in a broad distribution across most of North America from the treeline to the southern United States. It winters in the southern United States, and Central and South America. The autumn migration route of C. pusilla includes a non-stop flight over the Atlantic Ocean, whereas autumn route of A. macularia is largely over land. Because of this difference in their migratory paths and the visuo-spatial recognition tasks involved, we hypothesized that hippocampal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hippocampus; Neurons; Microglia; Shorebirds; Stereology; Morphometry.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000100603

Small-scale environmental enrichment and exercise enhance learning and spatial memory of Carassius auratus, and increase cell proliferation in the telencephalon: an exploratory study
Provedor de dados: 56
Autores: Abreu,C.C.; Fernandes,T.N.; Henrique,E.P.; Pereira,P.D.C.; Marques,S.B.; Herdeiro,S.L.S.; Oliveira,F.R.R.; Magalhães,N.G.M.; Anthony,D.C.; Melo,M.A.D.; Guerreiro-Diniz,C.; Diniz,D.G.; Picanço-Diniz,C.W.
Carassius auratus is a teleost fish that has been largely used in behavioral studies. However, little is known about potential environmental influences on its performance of learning and memory tasks. Here, we investigated this question in C. auratus, and searched for potential correlation between exercise and visuospatial enrichment with the total number of telencephalic glia and neurons. To that end, males and females were housed for 183 days in either an enriched (EE) or impoverished environment (IE) aquarium. EE contained toys, natural plants, and a 12-hour/day water stream for voluntary exercise, whereas the IE had none of the above. A third plus-maze aquarium was used for spatial and object recognition tests. Different visual clues in 2 of its 4 arms...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carassius auratus; Spatial learning and memory; Object recognition; Stereology; Telencephalon; Environmental enrichment.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500602

Early effects of estrogen on the rat ventral prostate
Provedor de dados: 56
Autores: García-Flórez,M.; Oliveira,C.A.; Carvalho,H.F.
Complex interactions between androgen and estrogen (E2) regulate prostatic development and physiology. We analyzed the early effects of a high single dose of E2 (25 mg/kg body weight) and castration (separately or combined) on the adult 90-day-old male Wistar rat ventral prostate. Androgen levels, prostate weight, and the variation in the relative and absolute volume of tissue compartments and apoptotic indices were determined for 7 days. Castration and exogenous E2 markedly reduced ventral prostate weight (about 50% of the control), with a significant reduction in the epithelial compartment and increased stroma. The final volume of the epithelium was identical at day 7 for all treatments (58.5% of the control). However, E2 had an immediate effect, causing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Estrogen; Prostate; Stereology; Tissue kinetics.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400002

Exendin-4 effects on islet volume and number in mouse pancreas
Provedor de dados: 60
Autores: Khorsandi,Layasadat; Nejad-Dehbashi,Fereshteh.
The aim of this study was to evaluate Exendin-4 (EX-4) effects on islet volume and number in the mouse pancreas. Thirty-two healthy adult male NMRI mice were randomly divided into control and experimental groups. EX-4 was injected intraperitoneally (i. p.) at doses of 0.25 (E1 group), 0.5 (E2 group), and 1 µg/kg (E3 group), twice a day for 7 consecutive days. One day after the final injection, the mice were sacrificed, and the pancreas from each animal dissected out, weighed, and fixed in 10% formalin for measurement of pancreas and islet volume, and determination of islet number by stereological assessments. There was a significant increase in the weight of pancreases in the E3 group. Islet and pancreas volumes in E1 and E2 groups were unchanged compared...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exendin-4/effects; Exendin-4/experimental study; Pancreas/effects fo Exendin-4; Islet/hypertrophy; Stereology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400014