Consulta: subjectFacets:"Stress response"
Registros recuperados: 17
Data/hora: 01/06/2024 11:52:54

Expression of stress-related genes in zebrawood (Astronium fraxinifolium, Anacardiaceae) seedlings following germination in microgravity
Provedor de dados: 74
Autores: Inglis,Peter W.; Ciampi,Ana Y.; Salomão,Antonieta N.; Costa,Tânia da S.A.; Azevedo,Vânia C.R.
Seeds of a tropical tree species from Brazil, Astronium fraxinifolium, or zebrawood, were germinated, for the first time in microgravity, aboard the International Space Station for nine days. Following three days of subsequent growth under normal terrestrial gravitational conditions, greater root length and numbers of secondary roots was observed in the microgravity-treated seedlings compared to terrestrially germinated controls. Suppression subtractive hybridization of cDNA and EST analysis were used to detect differential gene expression in the microgravity-treated seedlings in comparison to those initially grown in normal gravity (forward subtraction). Despite their return to, and growth in normal gravity, the subtracted library derived from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microgravity; Stress response; Germination; Suppression subtractive hybridization; Zebrawood.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100014

The many ways of coping with pressure
Provedor de dados: 5
Autores: Oger, Philippe M.; Jebbar, Mohamed.
The current paper reviews strategies employed by microorganisms from the deep biosphere, especially piezophiles (from the greek piezo = to press and philo = love), to cope with high hydrostatic pressure (HHP) prevailing in these biotopes. The aim of this review is not to constitute an exhaustive report of our current knowledge on the physiology of piezophiles, as recent reviews have covered part of this subject in detail (Abe, 2007; Lauro and Bartlett, 2008; Michiels et al., 2008; Simonato et al., 2006). Rather, we illustrate here, via a few chosen examples, where we stand in our understanding of the mechanisms employed by microorganisms from the depths of our planet to cope with HHP. (C) 2010 Institut Pasteur. Published by Elsevier Masson SAS. All rights...
Tipo: Text
Palavras-chave: Adaptation and evolution; Archaea; Deep biosphere; High hydrostatic pressure; Piezophile; Stress response.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00027/13797/11043.pdf

Are coping styles consistent in the teleost fish Sparus aurata through sexual maturation and sex reversal?
Provedor de dados: 5
Autores: Castanheira, Maria Filipa; Paramo, Sonia Martinez; Figueiredo, F.; Cerqueira, Marco; Millot, Sandie; Oliveira, Catarina C. V.; Martins, Catarina I. M.; Conceicao, Luis E. C.
Individual differences in behaviour and physiological responses to stress are associated with evolutionary adaptive variation and thus raw material for evolution. In farmed animals, the interest in consistent trait associations, i.e. coping styles, has increased dramatically over the last years. However, one of limitations of the available knowledge, regarding the temporal consistency, is that it refers always to short-term consistency (usually few weeks). The present study used an escape response during a net restraining test, previously shown to be an indicative of coping styles in seabream, to investigate long-term consistency of coping styles both over time and during different life history stages. Results showed both short-term (14 days) consistency...
Tipo: Text
Palavras-chave: Individual variation; Personality; Farm animals; Production; Sperm quality; Stress response.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00334/44485/44198.pdf

Quantitative genetic variation for post-stress cortisol and swimming performance in growth-selected and control populations of European sea bass (Dicentrarchus labrax)
Provedor de dados: 5
Autores: Vandeputte, Marc; Porte, J. D.; Auperin, B.; Dupont-nivet, M.; Vergnet, Alain; Valotaire, C.; Claireaux, Guy; Prunet, P.; Chatain, Beatrice.
Sea bass is a major species in Mediterranean aquaculture, and is now being subject to selective breeding programs for faster growth. In terrestrial species, it was demonstrated that fast growth may be linked to a correlated degradation of fitness traits. In this experiment, we evaluated 600 young sea bass from a factorial mating of 76 sires and 13 dams. The sires were from four genetic groups, wild (W), domesticated (D), and selected for growth (2 groups, M and P). The 600 offspring were submitted to two acute confinement stress challenges at 6 weeks intervals, and plasma cortisol at one hour post stress was measured. The same fish were also submitted to two swimming challenges at a 5 days interval, where the maximum sustained swimming speed (Umax) of each...
Tipo: Text
Palavras-chave: Aquaculture; Selective breeding; Stress response; Maximum sustained swimming speed; Heritability; Correlated response.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00308/41955/41218.pdf

Coping styles in farmed fish: consequences for aquaculture
Provedor de dados: 5
Autores: Castanheira, Maria Filipa; Conceicao, Luis E. C.; Millot, Sandie; Rey, Sonia; Begout, Marie-laure; Damsgard, Borge; Kristiansen, Tore; Hoglund, Erik; Overli, Oyvind; Martins, Catarina I. M.
Individual differences in physiological and behavioural responses to stressors are increasingly recognised as adaptive variation and thus raw material for evolution and fish farming improvements including selective breeding. Such individual variation has been evolutionarily conserved and is present in all vertebrate taxa including fish. In farmed animals, the interest in consistent trait associations, that is coping styles, has increased dramatically over the last years because many studies have demonstrated links to performance traits, health and disease susceptibility and welfare. This study will review (i) the main behavioural, neuroendocrine, cognitive and emotional differences between reactive and proactive coping styles in farmed fish; (ii) the...
Tipo: Text
Palavras-chave: Behavioural syndromes; Farm animals; Individual variation; Personality; Stress response.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00270/38097/37309.pdf

Microplastics induce dose-specific transcriptomic disruptions in energy metabolism and immunity of the pearl oyster Pinctada margaritifera
Provedor de dados: 5
Autores: Gardon, Tony; Morvan, Lucie; Huvet, Arnaud; Quillien, Virgile; Soyez, Claude; Le Moullac, Gilles; Le Luyer, Jeremy.
A combined approach integrating bioenergetics and major biological activities is essential to properly understand the impact of microplastics (MP) on marine organisms. Following experimental exposure of polystyrene microbeads (micro-PS of 6 and 10 μm) at 0.25, 2.5, and 25 μg L−1, which demonstrated a dose-dependent decrease of energy balance in the pearl oyster Pinctada margaritifera, a transcriptomic study was conducted on mantle tissue. Transcriptomic data helped us to decipher the molecular mechanisms involved in P. margaritifera responses to micro-PS and search more broadly for effects on energetically expensive maintenance functions. Genes related to the detoxification process were impacted by long-term micro-PS exposure through a decrease in...
Tipo: Text
Palavras-chave: Microbeads; Marine bivalve; Differentially expressed genes; Stress response; Energy-limited tolerance.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00640/75213/75351.pdf

Thermal biology of the deep-sea vent annelied Paralvinella grasslei: in vivo studies
Provedor de dados: 5
Autores: Cottin, D; Ravaux, J; Leger, N; Halary, S; Toullec, J; Sarradin, Pierre-marie; Gaill, F; Shillito, B.
The annelid Paralvinella grasslei is a deep-sea vent endemic species that colonizes the wall of active chimneys. We report here the first data on its thermal biology based on in vivo experiments in pressurized aquaria. Our results demonstrate that P. grasslei survives a 30 min exposure at 30 degrees C, and suggest that the upper thermal limit of this species is slightly above this temperature. The first signs of stress were noticed at 30 C, such as a significant increase in the animal's activity and the expression of HSP70 stress proteins. A preliminary investigation of the kinetics of stress protein expression surprisingly showed high levels of HSP70 proteins as late as 3.5 h after the heat shock. Finally, we provide here the first sequences for vent...
Tipo: Text
Palavras-chave: IPOCAMP; Annelids; Heat stress; Stress response; Heat shock proteins; Hydrothermal vents.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4570.pdf

Factors Involved in the Persistence of a Shiga Toxin-Producing Escherichia coil O157:H7 Strain in Bovine Feces and Gastro-Intestinal Content
Provedor de dados: 5
Autores: Segura, Audrey; Auffret, Pauline; Bibbal, Delphine; Bertoni, Marine; Durand, Alexandra; Jubelin, Gregory; Kerouredan, Monique; Brugere, Hubert; Bertin, Yolande; Forano, Evelyne.
Healthy cattle are the primary reservoir for O157:H7 Shiga toxin-producing E coil responsible for human food-borne infections. Because farm environment acts as a source of cattle contamination, it is important to better understand the factors controlling the persistence of E. coil O157:H7 outside the bovine gut. The E. coil O157:H7 strain MC2, identified as a persistent strain in French farms, possessed the characteristics required to cause human infections and genetic markers associated with clinical O157:H7 isolates. Therefore, the capacity of E. coil MC2 to survive during its transit through the bovine gastro-intestinal tract (GIT) and to respond to stresses potentially encountered in extra intestinal environments was analyzed. E. coil MC2 survived in...
Tipo: Text
Palavras-chave: Escherichia coli; STEC; EHEC; O157:H7; Biofilms; Stress response; Persistence.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00434/54588/55972.pdf

Impact of ecotourism on the fish fauna of Bonito region (Mato Grosso do Sul State, Brazil): ecological, behavioural and physiological measures
Provedor de dados: 83
Autores: Lima,Ana Carolina; Assis,Jorge; Sayanda,Diogo; Sabino,José; Oliveira,Rui F.
Bonito, in Mato Grosso do Sul State, is an important destination for ecotourism in Brazil and the main challenge of sustainable ecotourism here has been to accommodate and adjust the increase of tourism without compromising the ecological integrity of the local ecosystems. In this study we aimed to assess the environmental impact of ecotourism on the fish fauna of Sucuri River in Bonito by integrating ecological, behaviour and physiological criteria and using the fish species Crenicichla lepidota and Moenkhausia bonita as indicators. Two distinct sites were defined to collect data: (1) affected daily by ecotourism (Tourism) and (2) undisturbed (No Tourism). The "stationary point count" method was performed to assess variations in ecological parameters and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Behaviour; Cortisol; Crenicichla lepidota; Moenkhausia bonita; Stress response.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000100133

Physiological effects of gasoline on the freshwater fish Prochilodus lineatus(Characiformes: Prochilodontidae)
Provedor de dados: 83
Autores: Simonato,Juliana D.; Fernandes,Marisa N.; Martinez,Cláudia B. R.
The purpose of this work was to evaluate the effects of the water-soluble fraction of gasoline (WSFG) on the Neotropical freshwater fish Prochilodus lineatus. The WSFG was prepared by mixing gasoline in water (1:4) and animals were exposed for 6, 24 and 96h to 5% diluted WSFG or only to water. After exposure, blood was collected from the caudal vein and the gills were removed. The following parameters were analyzed: hematological (hemoglobin, hematocrit, number of red blood cells), osmo-ionic (plasma Na+, Cl- and K+ and plasma osmolarity), metabolic (total plasma proteins and glucose), endocrine (cortisol), density and distribution of chloride cells [CC] in the gills (immunohistochemistry), and branchial Na+/K+-ATPase (NKA) activity. Hemolysis was found to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chloride cells; Hematology; Hydrocarbons; Na+K+-ATPase; Osmoregulation; Stress response.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300683

Oxidative stress biomarkers and aggressive behavior in fish exposed to aquatic cadmium contamination
Provedor de dados: 83
Autores: Almeida,Jeane A.; Barreto,Rodrigo E.; Novelli,Ethel L. B.; Castro,Fabio J.; Moron,Sandro E.
The objective of this study was to investigate the possible link between cadmium exposure, hepatic markers of oxidative stress and aggressive behavior in Nile tilapia (Oreochromis niloticus). Fish were first exposed to 0.75 mg/L CdCl2 for 15 days (12 isolated fish for each group) and afterward a behavioral test was performed. Fish from the control and cadmium-exposed groups were paired for 1 h (6 pairs of fish per group) for determination of aggressiveness parameters. Immediately after the behavioral test, the animals were sacrificed and the liver was used to determine biochemical parameters. Cadmium decreased aggression in Nile tilapia. Subordinate animals exposed to cadmium showed decreased glutathione peroxidase (GSH-Px) activity compared to dominant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oreochromis niloticus; Stress response; Antioxidant enzymes; Social stress; Liver; Nile tilapia.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000100013

Exposure to 50-Hz electric fields on stress response initiated by infection with the protozoan parasite, Toxoplasma gondii, in mice
Provedor de dados: 19
Autores: Hori, Takuya; Harakawa, Shinji; Inoue, Noboru; Nagasawa, Hideyuki; Kariya, Tatsuya.
The present study was aimed to examine whether a 50-Hz electric field (EF) affects biological stress responses. Male C57BL/6J mice were exposed to either 50-Hz EF (45 kV/m) or given a sham exposure for 30 minutes per day for 1, 2 or 4 weeks and then either mock inoculated or inoculated with the protozoan parasite, Toxoplasma gondii (T. gondii) PLK strain. Survival rate was calculated in mice infected with T. gondii after exposure to EF and the serum antibody levels to heat shock protein 60 (Hsp-60), and mRNA levels for Hsp-60, IFN-γ and IL-12p35 in mouse peripheral leucocytes were determined. As a result, no statistically significant difference was found in survival rate between mice exposed to EF and those in the sham exposure group. When mice were...
Palavras-chave: 50-Hz; Electric field; Hsp-60; Stress response; Survival rate.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/997

Cloning and expression analysis of rubredoxin from cold-treated banana leaves
Provedor de dados: 85
Autores: Feng¹,RJ; Lu,LF; Yuan,KH; Cheng,P; Zhang,LL; Qi,JF; Ren,Y; Xu,XL; Zhang,XB; Zhou,LY; Zhang,YD.
A banana (Musa AAA, Cavendish subgroup cv. Brazil) cDNA encoding a putative rubredoxin-like protein (MaRd1) was obtained from total RNA isolated from cold-treated banana leaves using rapid amplification of cDNA ends (RACE) technique. MaRd1 cDNA contained 597 nucleotides encoding 198 amino acids in the open reading frame. MaRd1 protein showed 56% amino acid identity with that of Pyrococcus furiosus rubredoxin (P24297). A chloroplast transit peptide and a transmembrane region were detected at the N-terminus and the C-terminus, respectively, of the deduced amino acid sequence of MaRd1 gene. Southern blotting revealed the occurrence of at least two copies of MaRd1 in the banana genome. Real time quantitative RT-PCR analysis revealed that the expression of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rubredoxin; Expression; Banana; Stress response.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000200008

Effects of sedation during upper gastrointestinal endoscopy on endocrine response and cardiorespiratory function
Provedor de dados: 56
Autores: Yetkin,G.; Oba,S.; Uludag,M.; Paksoy,I.; Akgün,I.; Eren,N.
Upper gastrointestinal endoscopy is often accompanied by tachycardia which is known to be an important pathogenic factor in the development of myocardial ischemia. The pathogenesis of tachycardia is unknown but the condition is thought to be due to the endocrine response to endoscopy. The purpose of the present study was to investigate the effects of sedation on the endocrine response and cardiorespiratory function. Forty patients scheduled for diagnostic upper gastrointestinal endoscopy were randomized into 2 groups. While the patients in the first group did not receive sedation during upper gastrointestinal endoscopy, the patients in the second group were sedated with intravenous midazolam at the dose of 5 mg for those under 65 years or 2.5 mg for those...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stress response; Oxygen saturation; Sedation; Upper gastrointestinal endoscopy; Tachycardia.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200008

Absence of peripheral blood mononuclear cells priming in hemodialysis patients
Provedor de dados: 56
Autores: Santos,B.C.; Starobinas,N.; Barbuto,J.A.M.; Russo,M.; Schor,N.
As a consequence of the proinflammatory environment occurring in dialytic patients, cytokine overproduction has been implicated in hemodialysis co-morbidity. However, there are discrepancies among the various studies that have analyzed TNF-alpha synthesis and the presence of peripheral blood mononuclear cell (PBMC) priming in this clinical setting. We measured bioactive cytokine by the L929 cell bioassay, and evaluated PBMC TNF-alpha production by 32 hemodialysis patients (HP) and 51 controls. No difference in TNF-alpha secretion was observed between controls and HP (859 ± 141 vs 697 ± 130 U/10(6) cells). Lipopolysaccharide (5 µg/ml) did not induce any further TNF-alpha release, showing no PBMC priming. Paraformaldehyde-fixed HP PBMC were not cytotoxic to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokine; Stress response; Translational control; Transcriptional blockage; Priming; Membrane-anchored tumor necrosis factor.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200009

High pressure-sensitive gene expression in Lactobacillus sanfranciscensis
Provedor de dados: 56
Autores: Vogel,R.F.; Pavlovic,M.; Hörmann,S.; Ehrmann,M.A.
Lactobacillus sanfranciscensis is a Gram-positive lactic acid bacterium used in food biotechnology. It is necessary to investigate many aspects of a model organism to elucidate mechanisms of stress response, to facilitate preparation, application and performance in food fermentation, to understand mechanisms of inactivation, and to identify novel tools for high pressure biotechnology. To investigate the mechanisms of the complex bacterial response to high pressure we have analyzed changes in the proteome and transcriptome by 2-D electrophoresis, and by microarrays and real time PCR, respectively. More than 16 proteins were found to be differentially expressed upon high pressure stress and were compared to those sensitive to other stresses. Except for one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High pressure; Lactobacillus sanfranciscensis; Proteome; Transcriptome; Stress response.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800013

Brief daily postpartum separations from the litter alter dam response to psychostimulants and to stress
Provedor de dados: 56
Autores: Silveira,P.P.; Benetti,C. da Silva; Portella,A.K.; Diehl,L.A.; Molle,R. Dalle; Lucion,A.B.; Dalmaz,C.
Neonatal handling induces several behavioral and neurochemical alterations in pups, including decreased responses to stress and reduced fear in new environments. However, there are few reports in the literature concerning the behavioral effects of this neonatal intervention on the dams during the postpartum period. Therefore, the aim of the current study was to determine if brief postpartum separation from pups has a persistent impact on the dam's stress response and behavior. Litters were divided into two neonatal groups: 1) non-handled and 2) handled [10 min/day, from postnatal day (PND) 1 to 10]. Weaning occurred at PND 21 when behavioral tasks started to be applied to the dams, including sweet food ingestion (PND 21), forced swimming test (PND 28), and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feeding behavior; Neonatal handling; Psychostimulants; Stress response; Dam behavior.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000500426