Consulta: subjectFacets:"Sulfated macromolecules"
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Analysis of two drying methods on the yield and activity of sulfated polysaccharides extracted from Halymenia sp. (Rhodophyceae) - doi: 10.4025/actascibiolsci.v34i1.6961
Provedor de dados: 3
Autores: Rodrigues, José Ariévilo Gurgel; Rede Nordeste de Biotecnologia - RENÓRBIO; Vanderlei, Edfranck de Sousa Oliveira; Universidade Federal do Ceará - UFC; Quinderé, Ana Luíza Gomes; Universidade Federal do Ceará - UFC; Queiroz, Ismael Nilo Lino de; Universidade Federal do Ceará - UFC; Bessa, Érika Façanha; Universidade Federal do Ceará - UFC; Benevides, Norma Maria Barros; Universidade Federal do Ceará - UFC.
Sulfated polysaccharides (SPs) have attracted growing interest for various biotechnological applications. We evaluated the efficiency of two methods of drying SPs (M I and II) extracted from Halymenia sp Rhodophyceae in order to compare the yield, purification and anticoagulant activity. The total SPs (TSPs) were first extracted with papain in 100 mM sodium acetate (pH 5.0) containing cysteine and EDTA (5 mM). The TSPs obtained were dried in an oven (M I) or lyophilized (M II) and then examined by ion exchange chromatography (DEAE-cellulose) using the NaCl gradient technique. The fractions were analyzed by 0.5% agarose gel electrophoresis and the in vitro anticoagulant activity was evaluated by the activated partial thromboplastin time test using normal...
Palavras-chave: 2.08.01.00-9 Halymeniales; Sulfated macromolecules; Drying methods; Blood coagulation 2.08.01.00-9.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6961

Polissacarídeos sulfatados isolados das clorofíceas Caulerpa racemosa e Caulerpa cupressoides – extração, fracionamento e atividade anticoagulante - doi: 10.4025/actascibiolsci.v32i2.5923
Provedor de dados: 3
Autores: Rodrigues, José Ariévilo Gurgel; REDE NORDESTE DE BIOTECNOLOGIA; Vanderlei, Edfranck de Sousa Oliveira; UNIVERSIDADE FEDERAL DO CEARÁ; Quinderé, Ana Luíza Gomes; UNIVERSIDADE FEDERAL DO CEARÁ; Fontes, Bruno Pedrosa; UNIVERSIDADE FEDERAL DO CEARÁ; Benevides, Norma Maria Barros; UNIVERSIDADE FEDERAL DO CEARÁ.
A incidência de doenças cardiovasculares e os efeitos adversos da heparinoterapia têm motivado a busca por novos agentes terapêuticos e os polissacarídeos sulfatados (PS) de algas marinhas têm sido reportados como fontes alternativas para tal. Objetivou-se avaliar o potencial anticoagulante dos PS totais (PST) isolados e fracionados das clorofíceas Caulerpa racemosa e Caulerpa cupressoides. Inicialmente, os PST foram extraídos com papaína em tampão acetato de sódio 0,1 M (pH 5,0) contendo cisteína 5 mM e EDTA 5 mM, seguidos de fracionamento em coluna de troca iônica de DEAE-celulose com gradiente de NaCl. As frações obtidas foram analisadas por eletroforese em gel de agarose a 0,5% e a atividade anticoagulante, mensurada pelo tempo de tromboplastina...
Tipo: Bioprospecção
Palavras-chave: 2.08.00.00-2 algas verdes; Macromoléculas sulfatadas; Coagulação sanguínea; TTPA Química de macromoléculas green algae; Sulfated macromolecules; Blood coagulation; APTT.
Ano: 2010
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5923

Anticoagulant activity of a sulfated polysaccharide isolated from the green seaweed Caulerpa cupressoides
Provedor de dados: 52
Autores: Rodrigues,José Ariévilo Gurgel; Vanderlei,Edfranck de Sousa Oliveira; Bessa,Érika Façanha; Magalhães,Francisco de Araújo; Paula,Regina Célia Monteiro de; Lima,Vilma; Benevides,Norma Maria Barros.
The aim of this study was to evaluate certain molecular characteristics of a sulfated polysaccharide (SPs) with anticoagulant properties, isolated from Caulerpa cupressoides (Chlorophyta). Crude SPs were extracted by proteolytic digestion (papain), followed by ion-exchange chromatography on a DEAE-cellulose column. The fractions obtained were analyzed for molecular mass, 0.5% agarose gel electrophoresis and chemical composition. The activated partial thromboplastin time (APTT) test was applied using normal human plasma and standard heparin (HEP) (193 IU mg-1). The yield was ~ 3%, and the chromatography procedure separated the material into three different SP fractions (F I, F II and F III), eluted at the concentrations of 0.50, 0.75 and 1.00 M of NaCl,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marine algae; Sulfated macromolecules; Chemical analysis; APTT test.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400007

Antimicrobial effect of a crude sulfated polysaccharide from the red seaweed Gracilaria ornata
Provedor de dados: 52
Autores: Amorim,Rodrigo das Neves dos Santos; Rodrigues,José Ariévilo Gurgel; Holanda,Márjory Lima; Quinderé,Ana Luíza Gomes; Paula,Regina Célia Monteiro de; Melo,Vânia Maria Maciel; Benevides,Norma Maria Barros.
The aim of this study was to determine the yield, chemical composition, specific rotation (SR), infrared (IR) spectroscopy and the effect on bacterial growth of a crude sulfated polysaccharide (SP) from the red marine alga G. ornata (Go). Go-1 (25°C), Go-2 (80°C), and Go-3 (80°C) were sequentially extracted and yielded 9.2%. The contents of sulfate (5.88-10.3%) and proteins (0.1-3.7%) were small. The values of SR were [µ]D20°f -19.0, -51.0, and -56.5, respectively. IR spectrums showed the presence of galactose-4 sulfate and absence of 3,6-anydrogalactose-2 sulfate, galactose-6 sulfate and galactose-2 sulfate. SR and IR techniques confirmed SPs. Go-3 was tested on the growth of bacteria (Bacillus subtilis, Staphylococcus aureus, Enterobacter aerogens,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhodophyta; Sulfated macromolecules; Chemical analysis; Antimicrobial.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200001