Consulta: subjectFacets:"Suppression subtractive hybridization"
Registros recuperados: 8
Data/hora: 06/06/2024 14:17:35

Analysis of gene expression in Kalanchoe daigremontiana leaves during plantlet formation under drought stress
Provedor de dados: 69
Autores: Zhong,Tianxiu; Zhu,Chen; Zeng,Huiming; Han,Liebao.
Background: Kalanchoe daigremontiana is an attractive model system for the study of the molecular mechanisms of somatic embryogenesis and organogenesis competence due to its formation of plantlets with adventitious roots on the leaf margins that are derived from somatic embryos. The suppression subtractive hybridization technique was used to investigate gene expression during asexual reproduction. Leaves from plants subjected to drought stress provided the source of &amp;#8216;Tester&amp;#8217; DNA, and leaves from plants grown under normal conditions provided the &amp;#8216;Driver&amp;#8217; DNA for subtractive hybridization. Results: A total of 481 high quality ESTs were generated, which clustered into 390 unigenes. Of these unigenes, 132 grouped into 12...
Tipo: Journal article
Palavras-chave: Bioinformatics analysis; Real time PCR; Suppression subtractive hybridization.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600004

Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.)
Provedor de dados: 69
Autores: Tang,Yue Yi; Wang,Chuan Tang; Yang,Guan Pin; Feng,Tong; Gao,Hua Yuan; Wang,Xiu Zhen; Chi,Xiao Yuan; Xu,Ya Long; Wu,Qi; Chen,Dian Xu.
To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2ºC and 15ºC, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4%) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6%) showed no similarity to either hypothetical or known...
Tipo: Journal article
Palavras-chave: Chilling response; Peanut; Real-time quantitative PCR; Suppression subtractive hybridization.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500005

Identification of phase relative genes in tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis (Gracilariales, Rhodophyta)
Provedor de dados: 69
Autores: Ren,Xueying; Zhang,Xuecheng; Sui,Zhenghong.
Genes differentially expressed between tetrasporophytes and female gametophytes of Gracilaria/Gracilariopsis lemaneiformis were isolated by suppression subtractive hybridization (SSH) and screened by dot-blot macro-arrays. Different expression profiles of selected clones based on the results of dot-blot macro-arrays were verified using virtual Northern blots. Totally, 14 phase relative cDNAs had been isolated and sequence identified. Among them, seven cDNAs were respectively homologous to crucial metabolic enzymes, Rab GTPase, RP42 homolog, and two hypothetical proteins, while the rest did not have significant hits in the databases examined. The results of virtual Northern blots revealed that 11 cDNAs were differentially expressed between the two samples,...
Tipo: Journal article
Palavras-chave: Female gametophyte; Gracilaria/Gracilariopsis lemaneiformis; Suppression subtractive hybridization; Tetrasporophyte; Virtual Northern blot.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200006

Expression of stress-related genes in zebrawood (Astronium fraxinifolium, Anacardiaceae) seedlings following germination in microgravity
Provedor de dados: 74
Autores: Inglis,Peter W.; Ciampi,Ana Y.; Salomão,Antonieta N.; Costa,Tânia da S.A.; Azevedo,Vânia C.R.
Seeds of a tropical tree species from Brazil, Astronium fraxinifolium, or zebrawood, were germinated, for the first time in microgravity, aboard the International Space Station for nine days. Following three days of subsequent growth under normal terrestrial gravitational conditions, greater root length and numbers of secondary roots was observed in the microgravity-treated seedlings compared to terrestrially germinated controls. Suppression subtractive hybridization of cDNA and EST analysis were used to detect differential gene expression in the microgravity-treated seedlings in comparison to those initially grown in normal gravity (forward subtraction). Despite their return to, and growth in normal gravity, the subtracted library derived from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microgravity; Stress response; Germination; Suppression subtractive hybridization; Zebrawood.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100014

Differential expression of metallothionein type-2 homologues in leaves and roots of Black pepper (Piper nigrum L)
Provedor de dados: 74
Autores: Alex,Susan M.; Dicto,Jose; Purushothama,M.G.; Manjula,S.
Black pepper (Piper nigrum L.), member of the family Piperaceae is indigenous to India and is one of the most widely used spices in the world. In this paper we report the results of our attempts to identify a set of genes differentially expressed in the leaves of Piper nigrum, which could facilitate targeted engineering of this valuable crop. A PCR-based Suppression Subtractive Hybridization (SSH) technique was used to generate a leaf-specific subtracted cDNA library of Piper nigrum. A tester population of leaf cDNA was subtracted with a root derived driver cDNA. The efficiency of subtraction was confirmed by PCR analysis using the housekeeping gene actin. On sequence analysis, almost 30% of the clones showed homology to metallothionein type-2 gene. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Piper nigrum; Suppression subtractive hybridization; Metallothionein type-2; Real-time PCR analysis; Northern blotting.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300024

Study of interactions between flat oyster, Ostrea edulis, and the parasite Bonamia ostreae using flow cytometry and suppression subtractrive hybridization (SSH)
Provedor de dados: 5
Autores: Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Gagnaire, Beatrice; Chollet, Bruno; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting flat oysters Ostrea edulis. Control of bonamiosis requires a good knowledge of the parasite life cycle including host-parasite interactions. In this study, we investigated interactions between parasites and haemocytes by flow cytometry and Suppression Subtractive Hybridization (SSH). In both approaches, analyses were performed after 2 hours of contact between haemocytes and parasites purified from highly infected flat oysters. Flow cytometry analyses consisted in testing haemocyte activities including esterase activities, reactive oxygen species (ROS) production and phagocytosis after contact with live parasites and parasites inactivated by...
Tipo: Text
Palavras-chave: SSH; Suppression subtractive hybridization; Cytometry; Bonamia ostreae; Parasite; Ostrea edulis; Oyster.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/acte-3367.pdf

The identification of genes from the oyster Crassostrea gigas that are differentially expressed in progeny exhibiting opposed susceptibility to summer mortality
Provedor de dados: 5
Autores: Huvet, Arnaud; Herpin, Amaury; Degremont, Lionel; Labreuche, Yannick; Samain, Jean-francois; Cunningham, Charles.
Summer mortality associated with juveniles of the oyster Crassostrea gigas is probably the result of a complex interaction between the host, pathogens and environmental factors. Genetic variability in the host appears to be a major determinant in its sensitivity to summer mortality. Previously, divergent selection criteria based on summer survival have been applied to produce oyster families with resistant and susceptible progeny. In this paper, we describe the use of suppression subtractive hybridization to generate 150 C gigas clones that were differentially regulated between resistant and susceptible F2 progeny. The nucleotide sequence of these clones was determined. In 28%, the inferred amino sequence was found to match the products of known genes, 14%...
Tipo: Text
Palavras-chave: Suppression subtractive hybridization; Oysters; Differentially regulated genes; Bivalves; Bacterial challenge.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-651.pdf

Differential gene expression, induced by salicylic acid and Fusarium oxysporum f. sp. lycopersici infection, in tomato
Provedor de dados: 86
Autores: Amaral,Daniel Oliveira Jordão do; Lima,Marleide Magalhães de Andrade; Resende,Luciane Vilela; Silva,Márcia Vanusa da.
The objective of this work was to determine the transcript profile of tomato plants (Lycopersicon esculentum Mill.), during Fusarium oxysporum f. sp. lycopersici infection and after foliar application of salicylic acid. The suppression subtractive hybridization (SSH) technique was used to generate a cDNA library enriched for transcripts differentially expressed. A total of 307 clones was identified in two subtractive libraries, which allowed the isolation of several defense-related genes that play roles in different mechanisms of plant resistance to phytopathogens. Genes with unknown roles were also isolated from the two libraries, which indicates the possibility of identifying new genes not yet reported in studies of stress/defense response. The SSH...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lycopersicon esculentum; CDNA libraries; Complementary DNA; Gene expression; Salicylic acids; Suppression subtractive hybridization.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000800010