Consulta: subjectFacets:"Synthetic biology"
Registros recuperados: 4
Data/hora: 01/06/2024 13:58:51

Synthetic and minimalist vectors for Agrobacterium tumefaciens-mediated transformation of fungi
Provedor de dados: 74
Autores: Nora,Luísa Czamanski; Gonçales,Relber Aguiar; Martins-Santana,Leonardo; Ferreira,Beatriz Henriques; Rodrigues,Fernando; Silva-Rocha,Rafael.
Abstract We present a collection of minimalist binary vectors for transformation through ATMT applicable to several fungi species. pLUO plasmid binary vectors consist of a reporter module containing fluorescent proteins, mCherry or eGFP, flanked by a multiple cloning site and a transcription terminator site. They also present a synthetic gene allowing resistance to Hygromicin B flanked by alternate promoters, one for yeast and another for filamentous fungi. Left and right borders were added for Agrobacterium tumefaciens recognition, and a minimal broad-host range RK2 replication origin. Transformation was validated in the pathogenic fungus Paracoccidioides lutzii. Hence, we developed an efficient and reliable molecular tool for fungal transformation:...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Synthetic biology; Fungi; Agrobacterium tumefaciens; Transformation; Vectors.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300395

Biologia sintética.
Provedor de dados: 14
Autores: VASCONCELOS, M. J. V. de; FIGUEIREDO, J. E. F.
A Biologia Sintética, em franca expansão nos dias de hoje, consiste no uso de bioinformática e técnicas de engenharia genética e bioquímica com o objetivo de desenhar circuitos biológicos modulares, por meio do redirecionamento ou construção de novas rotas metabólicas e a criação de organismos artificiais, visando maximizar o seu funcionamento. Não se trata de uma técnica específica, mas de um conceito que pode utilizar diferentes abordagens de edição genômica para alcançar a meta final. Esse documento tem como objetivo fornecer uma visão abrangente desse novo ramo da biologia, de modo a ampliar a nossa percepção sobre as possibilidades futuras que podem ser vislumbradas sobre o desenvolvimento e as aplicações dessa nova abordagem científica.
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Genética; Gene; Synthetic biology; Genetics.
Ano: 2015
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1037794

Molecular biology of mycoplasmas: from the minimum cell concept to the artificial cell
Provedor de dados: 42
Autores: CORDOVA,CAIO M.M.; HOELTGEBAUM,DANIELA L.; MACHADO,LAÍS D.P.N.; SANTOS,LARISSA DOS.
ABSTRACT Mycoplasmas are a large group of bacteria, sorted into different genera in the Mollicutes class, whose main characteristic in common, besides the small genome, is the absence of cell wall. They are considered cellular and molecular biology study models. We present an updated review of the molecular biology of these model microorganisms and the development of replicative vectors for the transformation of mycoplasmas. Synthetic biology studies inspired by these pioneering works became possible and won the attention of the mainstream media. For the first time, an artificial genome was synthesized (a minimal genome produced from consensus sequences obtained from mycoplasmas). For the first time, a functional artificial cell has been constructed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma; Molecular biology; Microbial genetics; Synthetic biology; Gene transfer techniques.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200599

Synthetic Biology: opportunities for Chilean bioindustry and education
Provedor de dados: 48
Autores: Federici,Fernán; Rudge,Timothy J; Pollak,Bernardo; Haseloff,Jim; Gutiérrez,Rodrigo A.
In an age of pressing challenges for sustainable production of energy and food, the new field of Synthetic Biology has emerged as a promising approach to engineer biological systems. Synthetic Biology is formulating the design principles to engineer affordable, scalable, predictable and robust functions in biological systems. In addition to efficient transfer of evolved traits from one organism to another, Synthetic Biology offers a new and radical approach to bottom-up engineering of sensors, actuators, dynamical controllers and the biological chassis they are embedded in. Because it abstracts much of the mechanistic details underlying biological component behavior, Synthetic Biology methods and resources can be readily used by interdisciplinary teams to...
Tipo: Journal article
Palavras-chave: Synthetic biology; Bioengineering; Education; Engineering; Biotechnology.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400010