Consulta: subjectFacets:"Technetium-99m"
Registros recuperados: 34
Data/hora: 05/06/2024 10:36:12

Synthesis and antimicrobial evaluation of two peptide LyeTx I derivatives modified with the chelating agent HYNIC for radiolabeling with technetium-99m
Provedor de dados: 80
Autores: Fuscaldi,Leonardo Lima; Santos,Daniel Moreira dos; Pinheiro,Natália Gabriela Silva; Araújo,Raquel Silva; Barros,André Luís Branco de; Resende,Jarbas Magalhães; Fernandes,Simone Odília Antunes; Lima,Maria Elena de; Cardoso,Valbert Nascimento.
Abstract  Background  Current diagnostic methods and imaging techniques are not able to differentiate septic and aseptic inflammation. Thus, reliable methods are sought to provide this distinction and scintigraphic imaging is an interesting option, since it is based on physiological changes. In this context, radiolabeled antimicrobial peptides have been investigated as they accumulate in infectious sites instead of aseptic inflammation. The peptide LyeTx I, from the venom of Lycosa erythrognatha, has potent antimicrobial activity. Therefore, this study aimed to synthesize LyeTx I derivatives with the chelating compound HYNIC, to evaluate their antimicrobial activity and to radiolabel them with 99mTc.  Methods  Two LyeTx I derivatives, HYNIC-LyeTx I...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Septic and aseptic inflammation; Differential diagnosis; Antimicrobial peptides LyeTx I derivatives; MALDI-ToF-MS RP-HPLC; Technetium-99m; HYNIC EDDA Tricine.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100312

Effects of fenoprofen on the labeling of blood constituents with technetium-99m, the morphology of red blood cells and the plasmid
Provedor de dados: 52
Autores: Pereira,Marcia de Oliveira; Rocha,Gabrielle de Souza; Lombardi,Simone dos Santos; Geller,Mauro; Pereira,Mário José; Santos-Filho,Sebastião David; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
The aim of this work was to evaluate the effect of fenoprofen on the labeling of blood constituents with technetium-99m, on the morphology of red blood cells and on the plasmid DNA. Blood samples from Wistar rats were incubated with fenoprofen and the assay of labeling of blood constituents with technetium-99m (99mTc) was performed. Blood cells, plasma, soluble and insoluble fractions of blood cells and plasma were separated. The radioactivity in each fraction was counted and percentage of incorporated radioactivity (%ATI) was determined. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of the red blood cells (RBC) was evaluated. Plasmid (pBSK) was incubated with fenoprofen with stannous chloride, and agarose gel...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Technetium-99m; Blood; Morphology; Plasmid; Fenoprofen.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700022

Study of gels of molybdenum with cerium in the preparation of generators of 99Mo - 99mTc
Provedor de dados: 52
Autores: Moraes,Vanessa; Marczewski,Bárbara; Dias,Carla Roberta; Osso Junior,João Alberto.
99mTc has ideal nuclear properties for organ imaging in nuclear medicine, and it is obtained from the 99Mo-99mTc generator. Four different types of generators are available: chromatographic that uses 99Mo from fission of uranium; MEK solvent extraction; Tc2O7 sublimation; gel chromatographic. This work presents the preparation of gel generators of molybdenum with cerium and characterization of the gels: mass ratio between molybdenum and cerium, structure, size of particles and elution percentage of 99mTc after irradiating the gels. Eight gels were prepared at the same temperature of 50 ºC with concentrations of NaOH of 2 and 4 mol/L, mass ratio of 0.31 and 0.38 and final pH of 3.5 and 4.5. The analysis of the results proved that these gels are not adequate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radioisotopes; Technetium-99m; Molybdenum-99; Gel generator.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700007

Effect of an Arctium lappa (burdock) extract on the labeling of blood constituents with technetium-99m and on the morphology of the red blood cells
Provedor de dados: 52
Autores: Neves,Rosane de Figueiredo; Moreno,Silvana Ramos Farias; Rebello,Bernardo Machado; Caldas,Luiz Querino de Araújo; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario; Medeiros,Aldo da Cunha.
Arctium lappa (burdock) has been used to treat inflammatory processes. Blood constituents labeled with technetium-99m (99mTc) have been utilized in nuclear medicine. It was evaluated the influence of a burdock extract on the labeling of blood constituents with 99mTc and on the morphometry of red blood cells. Blood samples from Wistar rats were incubated with burdock extract and the radiolabeling procedure was carried out. Plasma and blood cells, soluble and insoluble fractions of plasma and blood cells were separated. The radioactivity in each fraction was counted and the percentages of radioactivity (%ATI) were determined. Morphology and morphometric (perimeter/area ratio) measurements of red blood cells (RBC) were performed. The incubation with burdock...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arctium lappa; Technetium-99m; Blood constituents; Morphometry.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600021

Effect of the extract of Ricinus communis L. on the osmotic fragility, labeling of red blood cells with Technetium-99m and morphology of the cells
Provedor de dados: 52
Autores: Mousinho,Kristiana Cerqueira; Correia,Marília Bezerra Libório; Silva,Jailson Oliveira da; Magnata,Simey de Souza Leão Pereira; Souza,Ivone Antônia de; Catanho,Maria Teresa Jansem de Almeida.
The aim of this study was to evaluate the influence of the proteic extract of R. communis on the cell physiology by the osmotic fragility, labeling of the blood elements with the 99mTc and cell morphology. To evaluate the osmotic fragility, the blood samples of the Wistar rats were incubated with the concentrations of R. communis and with the solutions of NaCl (0.4; 0.7; 0.9%). In the labeling of the blood elements procedure, the rat blood was treated with a solution of Tc-99m and TCA at 5%, determining the rate of radioactivity (%ATI) in the plasma (P) and in the red blood cells (RBC). The soluble and insoluble fractions of the plasma were also evaluated. The cells morphology submitted to the extract was evaluated by the optical microscopy (x40). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ricinus communis L.; Labeling of red blood cells; Osmotic fragility; Technetium-99m; Morphology of cells.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600008

Effects of Chrysobalanus icaco on the labeling of blood constituents with technetium-99m and on the shape of the red blood cells
Provedor de dados: 52
Autores: Presta,Giuseppe Antonio; Santos-Filho,Sebastião David; Paoli,Severo de; Giani,Tania Santos; Maiworm,Adalgisa Ieda; Brandão-Neto,José; Medeiros,Aldo da Cunha; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
Chrysobalanus icaco (abajeru; C.icaco) is recommended in the treatment of diabetes and other clinical disorders. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this study was to verify the effects of an abajeru extract on the labeling of blood constituents with 99mTc and on the shape of red blood cells (RBC). Blood samples(Wistar rats) were incubated with abajeru extract and the labeling of blood constituents with 99mTc and morphology of RBC were carried out. The results showed significant (P&lt;0.05) alteration of labeling of blood constituents with 99mTc and the morphometry (perimeter/area ratio) of the RBC in presence of the extract. These data suggest that this abajeru extract could alter the labeling of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Red blood cells; Technetium-99m; Morphometry; Chrysobalanus icaco.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600018

The male reproductive system and the effect of an extract of a medicinal plant (Hypericum perforatum) on the labeling process of blood constituents with technetium-99m
Provedor de dados: 52
Autores: Santos-Filho,Sebastião David; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
Hypericum perforatum (hiperico) is a plant that has been used to treat diseases and also inhibits rat and human vas deferens contractility. In nuclear medicine, stannous chloride (SnCl2) is used as a reducing agent to obtain radiopharmaceuticals labeling with technetium-99m. As the SnCl2 seems to have adverse effects related with the reproductive performance of male rabbits as well as the human consumption of hiperico might affect sexual function. In the present work, consistent results show significant changes on the blood constituents labeled by technetium-99m obtained from young rats under the effect of an hiperico extract as opposed to blood samples equally treated taken from elderly rat.. Supposedly, this extract could protect the male reproductive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stannous chloride; Young animals; Elderly animals; Sexual function; Technetium-99m; Hypericum perforatum.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600011

Evaluation of possible failure of the mononuclear phagocyte system after total splenectomy in rats
Provedor de dados: 52
Autores: Marques,Ruy Garcia; Petroianu,Andy; Oliveira,Márcia Betânia Nunes de; Bernardo-Filho,Mário; Portela,Margareth Crisóstomo.
Young and adult Wistar rats were submitted to total splenectomy and compared to animals not submitted to any surgical manipulation in order to evaluate the phagocytic function of spleen. The animals were infected with Escherichia coli labeled with technetium-99m and killed 20 minutes later. Liver, lung, spleen and a blood clot sample were taken. No significant differences were found in the percentage of bacterial radioactivity uptake in mononuclear phagocyte system (MPS) organs in young and adult splenectomized rats. However, phagocytosis index by macrophages of MPS organs was smaller in splenectomized animals than in control group. Splenectomized rats were associated with a higher blood bacterial radioactivity uptake than animals of the control group...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spleen; Splenectomy; Technetium-99m; Escherichia coli; Phagocytosis; Mononuclear phagocyte system.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000200006

Effects of Cinnamomum zeylanicum treatment on radiolabeling of blood bonstituents and morphology of red blood cells in Wistar rats
Provedor de dados: 52
Autores: Benarroz,Mônica Oliveira; Rocha,Gabrielle de Souza; Pereira,Márcia Oliveira; Geller,Mauro; Fonseca,Adenilson de Souza da; Presta,Giuseppe Antonio; Bernardo-Filho,Mario.
The aim of this study was to evaluate the effect of in vivo treatment with an aqueous cinnamon extract on the labeling of blood constituents with 99mTc and on the morphology of red blood cells from Wistar rats. Animals were treated with cinnamon extract at different doses and for different periods of time. As controls, animals treated with 0.9% NaCl. Labeling of blood constituents with 99mTc was performed. Plasma, blood cells and insoluble fractions were isolated. Radioactivity in each fraction was counted and the percentage of radioactivity (%ATI) was calculated. Also, blood smears were prepared to morphological analysis of red blood cells from. Data showed that in vivo cinnamon extract did not significantly (p&gt;0.05) modify the %ATI of blood...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood constituents; Cinnamomum zeylanicum; Technetium-99m.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700023

Effect of tripanosomicide benznidazole (Rochagan®) on the biodistribution of sodium pertechnetate (Na99mTcO4) in Wistar rats
Provedor de dados: 52
Autores: Barbosa,Vanessa Santos de Arruda; Holanda,Maria de Carvalho Xavier; Silva,Roseane Pereira da; Oliveira,Daniel Pereira de; Silva Júnior,Maurício Ferreira da; Oliveira,Elias Herculano de; Spyrides,Maria Helena Constantino; Medeiros,Aldo Cunha.
Benznidazole, a drug with specific anti-Trypanosoma cruzi activity, is used in the treatment of Chagas' disease. The radiopharmaceutical sodium pertechnetate (Na99mTcO4) is used to obtain diagnostic images of the stomach, thyroid, parathyroids, salivary glands, brain and in the study of esophageal reflux and blood flow. This study aimed at evaluating in vivo the influence of benznidazole treatment on the sodium pertechnetate biodistribution in Wistar rats. The percentage of radioactivity per gram (%ATI/g) of various organs (brain, heart, esophagus, stomach, small intestine, large intestine, spleen, liver, muscle and blood) was determined. Comparing the treated rats with the controls, we observed that sodium pertechnetate biodistribution did not change when...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antiparasite; Radiopharmaceutical; Technetium-99m; Benznidazole; Biodistribution; Trypanosoma cruzi.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700028

Preparation of crotalus venom radiolabeled with technetium-99m as a tool for biodistribution study
Provedor de dados: 52
Autores: Pujatti,Priscilla Brunelli; Simal,Carlos Jorge Rodrigues; Santos,Raquel Gouvêa dos.
Technetium-99m (99mTc) has been the radionuclide of choice for nuclear medicine procedures and experimental research. Because of its optimal nuclear properties, 99mTc is suitable for high efficiency detection with the advantage of reduced radiological waste. Crotalus venom (CV) has been shown to reduce tumors in clinical studies and tissue distribution studies are very important for clinical use. The goal of this work was to obtain CV labeled with 99mTc which preserves its biological activity. After labeling, biological activity was assessed by hemolytic activity evaluation. Labeled and crude venom caused indirect hemolysis provided that the incubation medium contained an exogenous source of lecithin. High yield radiolabeled-CV was obtained and biological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Technetium-99m; Labeling; Hemolytic activity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700002

The effect of an extract from Ganoderma lucidum (reishi) on the labeling of blood constituents with technetium-99m and on the survival of Escherichia coli
Provedor de dados: 52
Autores: Agostinho,Raquel Terra; Santos-Filho,Sebastião David; Fonseca,Adenilson de Souza da; Missailidis,Sotiris; Bernardo-Filho,Mario.
This study evaluated effects of an aqueous extract of Ganoderma lucidum (reishi) on the labeling of blood constituents with technetium-99m (99mTc) and on the survival of cultures of Escherichia coli treated with stannous chloride. Blood samples from Wistar rats were treated with reishi extract, radiolabeling procedure was performed, plasma (P), blood cells (BC) and insoluble (IF) and soluble (SF) fractions of P and BC were separated. The radioactivity was counted for the determination of the percentages of radioactivity (%ATI). Cultures of Escherichia coli AB1157 were treated with stannous chloride in the presence and absence of reishi extract. Blood samples and bacterial cultures treated with NaCl 0.9% were used as controls. Data indicated that reishi...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood constituents; Escherichia coli; Ganoderma lucidum; Stannous chloride; Technetium-99m.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700025

An extract of a formula used in the traditional chinese medicine (Buzhong Yi Qi Wan) alters the labeling of blood constituents with technetium-99m
Provedor de dados: 52
Autores: Giani,Tania Santos; Paoli,Severo de; Presta,Giuseppe Antonio; Maiworm,Adalgisa Ieda; Santos-Filho,Sebastião David; Brandão-Neto,José; Medeiros,Aldo da Cunha; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
Buzhong Yi Qi Wan (Buzhong) is a medicinal herb widely used in Traditional Chinese Medicine to treat the digestive and circulatory systems. Red blood cell and plasma proteins labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this work was to investigate the effects of an aqueous Buzhong extract on the labeling of blood constituents with 99mTc. Heparinized blood (Wistar rats) was incubated in vitro with different Buzhong extract concentrations and 99mTc-labeling was performed. Plasma (P) and blood cells (BC) were separated and soluble (SF-P, SF-BC) and insoluble (IF-P, IF-BC) fractions were isolated. The radioactivity on blood constituents was determined and the percentage of incorporated radioactivity (%ATI) was calculated....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Technetium-99m; Red blood cell; Oxidant agent; Radiobiocomplex; Plasma proteins; Buzhong Yi Qi Wan.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600013

Effect of an extract of Centella asiatica on the biodistribution of sodium pertechnetate (Na99mTcO4) and on the fixation of radioactivity on blood constituents
Provedor de dados: 52
Autores: Diniz,Claudia Leite; Carmo,Fernanda Santos do; Almeida,Danielle Santos de; Santos-Filho,Sebastião David; Missailidis,Sotiris; Fonseca,Adenilson de Souza da; Bernardo Filho,Mario.
This study evaluates the effects of an acute treatment with a Centella asiatica (CA) extract on the biodistribution of the radiopharmaceutical Na99mTcO4 and on the fixation of technetium-99m on blood constituents. Wistar rats were treated with CA extract and, 1 hour after, Na99mTcO4 was administered; organs/tissues were withdrawn and weighted. The radioactivity was counted to calculate the percentage of activity per gram (%ATI/g). Also, blood samples were withdrawn, plasma (P), blood cells (BC), insoluble fraction (IF) and soluble fractions of P and BC were isolated and the radioactivity was counted to calculate the percentage of activity (%ATI). Data indicated that the acute treatment with CA extract changed significantly (p&lt;0.05) the %ATI/g in several...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodistribution; Centella asiatica; Blood; Plasma; Technetium-99m.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700035

Effect of a peel passion fruit flour (Passiflora edulis f. flavicarpa) extract on the labeling of blood constituents with technetium-99m and on the morphology of red blood cells
Provedor de dados: 52
Autores: Rebello,Bernardo Machado; Moreno,Silvana Ramos Farias; Ribeiro,Camila Godinho; Neves,Rosane de Figueiredo; Fonseca,Adenilson de Souza da; Caldas,Luis Querino de Araújo; Bernardo-Filho,Mario; Medeiros,Aldo da Cunha.
Passiflora edulis f. flavicarpa (maracuja) is a fruit consumed in Brazil and worldwide. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The effect of P. flavicarpa extract on the radiolabeling of blood constituents and on red blood cells morphology was evaluated. Blood samples from Wistar rats was incubated with P. flavicarpa extract. After that, the labeling of blood constituents with 99mTc was carried out. Samples of plasma and blood cells were precipitated with trichloroacetic acid to isolate the soluble and insoluble fractions of plasma and blood cells. The radioactivity in each fractions was counted and the percentage of radioactivity was determined. Blood smears were also prepared to morphological evaluation and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Labeling; Blood; Passiflora edulis f. flavicarpa; Radiobiocomplexes; Technetium-99m.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600019

Effects of clove (Caryophyllus aromaticus L.) on the labeling of blood constituents with technetium-99m and on the morphology of red blood cells
Provedor de dados: 52
Autores: Paoli,Severo de; Giani,Tania Santos; Presta,Giuseppe Antonio; Pereira,Marcia Oliveira; Fonseca,Adenilson de Souza da; Brandão-Neto,José; Medeiros,Aldo da Cunha; Santos-Filho,Sebastião David; Bernardo-Filho,Mário.
Clove (Caryophyllus aromaticus L.) has been used for clinical procedures. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this work was to evaluate the effects of clove extract on the labeling blood constituents with 99mTc and on the morphology of red blood cells. Blood samples were incubated with clove, stannous chloride and 99mTc. Plasma, blood cells, insoluble fractions of plasma and blood cells were separated. The radioactivity was counted and percentage of radioactivity (%ATI) to each blood fraction was calculated. The shape and morphometric parameter (perimeter/area ratio) were evaluated. Clove extract altered significantly (p&lt;0.05) the %ATI of blood constituents and the shape of red blood cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Technetium-99m; Blood constituents; Red blood cells; Morphology; Caryophyllus aromaticus L.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600022

An aqueous extract of Vitex agnus castus alters the labeling of blood constituents with technetium-99m
Provedor de dados: 52
Autores: Costa,Maria Regina de Macedo; Ribeiro,Camila Godinho; Santos-Filho,Sebastião David; Neves,Rosane de Figueiredo; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario; Catanho,Maria Teresa Jansem de Almeida.
The development of experimental assays to study properties of herbal medicine is worthwhile. Vitex agnus castus (VAC) is utilized in popular medicine and some actions have been attributed to its extract. Blood cells (BC) and plasma proteins are labeled with technetium-99m (Tc-99m) and have been used in nuclear medicine, as in basic research. This procedure uses a reducing agent and stannous ion is utilized. There are reports that drugs can alter this labeling process. The aim of this work was to evaluate the influence of an aqueous extract of VAC on the labeling of blood constituents with Tc-99m. Blood was incubated with VAC, stannous chloride and Tc-99m, as sodium pertechnetate, and centrifuged. Samples of BC and plasma were separated, aliquots of BC and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidant properties; Plasma proteins; Red blood cells; Technetium-99m; Vitex agnus-castus.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600023

Evaluation of the effect of an extract of sabugueiro (Sambucus australis) on the labeling of blood constituents with technetium-99m
Provedor de dados: 52
Autores: Ribeiro,Camila Godinho; Rebello,Bernardo Machado; Neves,Rosane de Figueiredo; Santos-Filho,Sebastião David; Fonseca,Adenilson de Souza da; Medeiros,Aldo da Cunha; Bernardo-Filho,Mario; Catanho,Maria Teresa Jansem de Almeida.
Sambucus australis (sabugueiro) has been used to treat inflammatory and rheumatologic disorders. Blood constituents labeled with technetium-99m (99mTc) have been used in nuclear medicine to obtain diagnostic images. The aim of this work was to evaluate the effect of a sabugueiro extract on the labeling of blood cells with 99mTc. Blood samples from Wistar rats were incubated with sabugueiro extract and the radiolabeling assay of blood constituents was carried out. After centrifugation, samples of plasma and blood cells were separated. Aliquots of plasma and blood cells were precipitated with trichloroacetic acid and centrifuged to isolate soluble and insoluble fractions. The radioactivity in each fraction was counted and the percentage of activity (%ATI)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sambucus australis; Blood constituents; Technetium-99m.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600020

Consequences of the magnetic field, sonic and radiofrequency waves and intense pulsed light on the labeling of blood constituents with technetium-99m
Provedor de dados: 52
Autores: Meyer,Patricia Froes; Santos-Filho,Sebastião David; Ronzio,Oscar Ariel; Bonelli,Ludmila; Fonseca,Adenilson de Souza da; Costa,Iris do Ceu Clara; Brandão Neto,José; Medeiros,Aldo da Cunha; Bernardo-Filho,Mario.
Sources of magnetic field, radiofrequency and audible sonic waves and pulsed light have been used in physiotherapy to treat different disorders. In nuclear medicine, blood constituents(Bl-Co) are labeled with technetium-99m (99mTc) are used. This study evaluated the consequences of magnetic field, radiofrequency and audible sonic waves and intense pulsed light sources on the labeling of Bl-Co with 99mTc. Blood from Wistar rats was exposed to the cited sources. The labeling of Bl-Co with 99mTc was performed. Blood not exposed to the physical agents was used(controls). Data showed that the exposure to the different studied sources did not alter significantly (p&gt;0.05) the labeling of Bl-Co. Although the results were obtained with animals, the data suggest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood constituents; Magnetic field; Sonic and radiofrequency waves; Technetium-99m.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600014

Experimental models using radionuclides for assessment of the effects of natural drugs
Provedor de dados: 52
Autores: Moreno,Silvana Ramos Farias; Carvalho,Jorge José de; Rebello,Bernardo; Rocha,Emely Kazan; Timóteo,Margareth de Oliveira; Olej,Beni; Bernardo Filho,Mário; Caldas,Luiz Querino de Araújo.
The aim of this study was to evaluate the effect of the extracts of Nectandra membranacea (N. membranacea), Ginkgo biloba (EGb) and Passiflora (PEF) on the morphology of red blood cells (RBC), on the biodistribution of sodium pertechnetate (99mTcO4Na), on the morphology of duodenum and on the labeling of blood constituents (BC, IF-P, IF-BC) with technetium-99m (Tc-99m). Morphometry studies also were performed. The results show that EGb promotes alteration of the labeling of BC, IF-P and IF-BC (p&lt;0.05). The N. membranacea extract does not promote significant alteration of the radiolabeling, and PEF extract alters the IF-P labeling. N. membranacea, EGb and PEF extracts were able to alter the RBC morphology (P&lt;0.05). N. membranacea extract and EGb...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nectandra membranacea (N. membranacea); Ginkgo biloba (EGb); Passiflora edulis flavicarpa (PEF); Blood constituents (BC) labeling; Technetium-99m; Biodistribution.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700027

Is nuclear medicine really safe?
Provedor de dados: 52
In nuclear medicine, radiation absorbed dose estimates calculated by standard models at the whole body or organ are very low. At cellular level, however, the heterogeneity of radionuclide distributions of radiation dose patterns may be significant. We present here absorbed doses at cellular level and evaluate their possible impact on the usually assumed risk/benefit relationships in nuclear medicine studies. The absorbed dose values calculated are surprisingly high, and are difficult to interpret. In the present study, we show calculated doses at the cellular level and discuss possible biological consequences, for two radiopharmaceuticals labelled with technetium-99m: human serum albumin microspheres used for pulmonary scintigrapies and HMPAO used to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Technetium-99m; Absorbed doses; Biological effects.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500016

Ultrastructural analysis of kidney, liver and duodenum isolated from treated rats with Ginkgo Biloba extract and effects of this medicinal plant on the biodistribution of the padiopharmaceutical sodium pertechnetate
Provedor de dados: 52
Autores: Moreno,Silvana Ramos Farias; Carvalho,Jorge José de; Nascimento,Ana Lúcia; Arnobio,Adriano; Olej,Beni; Timóteo,Margareth de Oliveira; Caldas,Luiz Querino de Araújo; Bernardo Filho,Mário.
Ginkgo biloba extract (EGb) has been used to treat memory and concentration deficits, acts as platelet activating factor antagonism and prevents against damages caused by free radicals. EGb is a standardized extract that contains 24% flavonoids and 6% terpenoids. The aim of this work was to evaluate the possible influence of an EGb on the ultrastructure of some organs isolated from rats and on the biodistribution of sodium pertechnetate (99mTcO4Na). The animals were treated with EGb and after six days, received 99mTcO4Na. The organs were isolated and fixed for ultrastructural analysis. The results showed that EGb has modified the ultrastructure of kidney, liver and duodenum and altered the biodistribution of 99mTcO4Na (P&lt;0.05). It is speculated that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ginkgo biloba; Technetium-99m; Biodistribution; Morphology; Ultrastructure.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700030

In Vitro study of Vellozia pusilla pohl (Velloziaceae), a Brazilian plant species: antitumoral activity and labeling of blood elements
Provedor de dados: 52
Autores: Dantas,Ana Letícia Almeida; Valente,Lígia Maria Marino; Morais,Lilia Aparecida Salgado de; Feliciano,Glaucio; Bernardo-Filho,Mário.
Vellozia pusilla Pohl is a species of the botanic family Velloziaceae that occurs in the subtropical regions of South America and, although it lives under conditions of high solar irradiation and low water availability, shows great longevity. The methanol extract of roots, stem and leaf sheaths of this species showed an antitumoral activity through the inhibition of the enzyme Topoisomerase I when analyzed by an in vitro bioassay employing DNA repair or recombination deficient mutants of the yeast Saccharomyces cerevisiae. In the evaluation of the effect of Vellozia pusilla methanol extract on the labeling of RBC, blood of mice was treated with 99mTc tracer solutions. The percentage of radioactivity (%ATI) bound to plasma (P) and blood cells (BC) was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drug interaction; Antitumoral activity; Technetium-99m; Blood elements; Velloziaceae.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700008

Effect of an extract of Artemisia vulgaris L. (Mugwort) on the in vitro labeling of red blood cells and plasma proteins with technetium-99m
Provedor de dados: 52
Autores: Terra,Danielle Amorim; Amorim,Lúcia de Fátima; Catanho,Maria Tereza Jansen de Almeida; Fonseca,Adenilson de Souza da; Santos-Filho,Sebastião David; Brandão-Neto,José; Medeiros,Aldo da Cunha; Bernardo-Filho,Mario.
The aim of this work was to evaluate the effect of an extract of the Artemisia vulgaris L. (mugwort) on the labeling of blood constituents with technetium-99m (99mTc). Blood samples from Wistar rats were incubated with a mugwort extract and the radiolabeling of blood constituents was carried out. Plasma and blood cells were separated by centrifugation. Aliquots of plasma and blood cells were also precipitated with trichloroacetic acid and centrifuged to isolate soluble and insoluble fractions of plasma and blood cells. Radioactivity in each fraction was counted and the percentages of radioactivity (%ATI) was calculated. Mugwort extract decreased significantly (p&lt;0.05) the %ATI on the blood compartments and on the blood cells proteins (insoluble...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artemisia vulgaris L.; Technetium-99m; Blood constituents; Labeling.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600015

Acetylsalicylic acid and labeling of blood constituents with technetium-99m
Provedor de dados: 52
Autores: Fonseca,Adenilson de Souza da; Frydman,Jacques Natan Grinapel; Rocha,Vanessa Câmara da; Bernardo-Filho,Mario.
Acetylsalicylic acid is the drug most used an anti-inflammatory agent and for secondary prevention of thrombotic phenomenon. Drugs can modify the labeling of blood constituents with technetium-99m (99mTc). The aim of this work was to evaluate the effect of in vitro or in vivo assays with acetylsalicylic acid on the labeling of the blood constituents with 99mTc. In vitro assay was performed with samples of whole blood from Wistar rats incubated with acetylsalicylic acid (1.0 mg/ml) for one hour before the 99mTc-labeling process. For in vivo assay, Wistar rats were treated with acetylsalicylic acid (1.5 mg/kg) during one hour, and the whole blood was withdrawn for the 99mTc-labeling process. Saline was used in control groups. Data showed that the fixation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetylsalicylic Acid; Blood Constituents; Technetium-99m.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700024

Effects of chronic sucralose sweetener on the labeling of blood constituents with technetium-99m, morphology of red blood cells and the biodistribution of sodium pertechnetate in rats
Provedor de dados: 52
Autores: Rocha,Gabrielle de Souza; Pereira,Marcia de Oliveira; Benarroz,Mônica de Oliveira; Frydman,Jacques Natan Grinapel; Garcia-Pinto,Angélica Beatriz; Pereira,Mário José; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
This work evaluates effects of the sweetener with sucralose on the labeling of blood constituents with technetium-99m (99mTc), on the morphology of red blood cells (RBC) and on the biodistribution of sodium pertechnetate in Wistar rats. Animals were treated with sweetener for 8 days. Blood samples were withdrawn and the assay of labeling of blood constituents with 99mTc was performed. Blood cells (BC) and plasma (P) were isolated. Aliquots of BC and P were also precipitated, soluble and insoluble fractions separated. The radioactivity in each fraction was counted and percentage of incorporated radioactivity (%ATI) determined. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of the RBC was evaluated under optical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodistribution; Blood; Morphology; Sweetener; Technetium-99m.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700021

Effect of medicinal plants on the parasitemia of Trypanosoma cruzi and on the biodistribution of sodium pertechnetate (Na99mTcO4)
Provedor de dados: 52
Autores: Silva,Roseane Pereira da; Holanda,Cecília Maria de Carvalho Xavier; Barbosa,Vanessa Santos de Arruda; Oliveira,Daniel Pereira de; Lima,Natália Alves; Câmara,Antônia Cláudia Jácome da; Medeiros,Aldo da Cunha; Constantino,Maria Helena Spyrides.
Artemisia vulgaris (AV) is an antihelmintic and antimalarial drug; Aloe vera(babosa) acts as antidiabetic, laxative and anti-inflammatory;Benznidazole (BZ) is a trypanocidal of Trypanosoma cruzi (TC). Technetium-99m (99mTc) has been used in nuclear medicine to obtain diagnostic images. This study evaluated the plant effects in TC parasitemia and on the biodistribution of 99mTc in mice. Twenty mice were infected by TC. At the peak of parasitemia, 5 mice received babosa; 5 received AV and 5 received BZ. The parasitemia was determined at 0, 2, 4 and 6 h of drugs administration. Five infected mice without drugs, 5 mice without TC and the group treated with AV, received 99mTc. The radioactivity was calculated. Infected mice that received babosa reduced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aloe vera; Artemisia vulgaris; Technetium-99m; Trypanosoma cruzi; Parasitemia; Biodistribution.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700034

Influence of an extract of Juglans regia on the growth of Escherichia coli, on the electrophoretic profile of plasmid DNA and on the radiolabeling of blood constituents
Provedor de dados: 52
Autores: Santos-Filho,Sebastião David; Diniz,Claudia Leite; Carmo,Fernanda Santos do; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
The aim of this work was to study the influence of a walnut (Juglans regia) extract on the growth of Escherichia coli (E. coli) AB1157, on the plasmid DNA topology and on the labeling of blood constituents with technetium-99m (99mTc). An E. coli AB1157 culture, in stationary phase, was incubated with walnut and the growth of the culture was evaluated by optical density at 600 nm for 7 hours. Plasmid DNA samples were incubated with SnCl2 in presence or absence of walnut for 40 minutes, 0.8% agarose gel electrophoresis was performed, the gel was stained and the plasmid topological forms were visualized. Blood samples from Wistar rats were incubated with walnut extract and an assay of labeling of blood constituents with technetium-99m (99mTc) was performed....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; DNA; Electrophoresis; Stannous chloride; Technetium-99m; Juglans regia.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700026

Uncaria tomentosa extract: evaluation of effects on the in vitro and in vivo labeling of blood constituents with technetium-99m
Provedor de dados: 52
Autores: Moreno,Silvana Ramos Farias; Carvalho,Jorge José de; Nascimento,Ana Lúcia; Olej,Beni; Rocha,Emely Kazan; Arnobio,Adriano; Bernardo-Filho,Mário; Caldas,Luiz Querino de Araújo; Honeycut,Hayden.
The influence (in vivo and in vitro) of an Uncaria tomentosa extract (Cats claw) on the labeling of red blood cells (RBCs) and plasma and cellular proteins with technetium-99m (Tc-99m) was evaluated. For the in vivo treatment, animals were treated with Cats claw. For the in vitro treatment, heparinized blood was incubated with Cats claw before the addition of stannous chloride (SnCl2) and Tc-99m. Samples of plasma (P) and RBCs were separated and also precipitated with trichloroacetic acid. The soluble and insoluble fractions of P and RBCs were isolated. The analysis of the results of the in vivo study, indicates that there is no significant alteration on the uptake of Tc-99m by the blood constituents, but it significantly decrease (p&lt;0.05) the labeling...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Uncaria tomentosa; Technetium-99m; Radiolabeling; Blood constituents.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700024

Novel Tc-99m radiotracers for brain imaging
Provedor de dados: 52
Autores: Boschi,Alessandra; Pasquali,Micol; Uccelli,Licia; Duatti,Adriano.
A novel class of Tc-99m complexes able to cross the blood brain barrier has been investigated and described here. These compounds are formed by reacting the bis-substituted nitrido precusors [99mTc(N)(PS)2] (PS = phosphino-thiol ligand) with triethylborane (BEt3) under strictly anhydrous conditions and using non-aqueous solvents. The molecular structure of these tracers was not fully established, but preliminary experimental evidence suggests that they result from the interaction of the Lewis base [99mTc(N)(PS)2] with the Lewis acid BEt3, which leads to the formation of the novel -B-N&lt;FONT FACE=Symbol&gt;º&lt;/FONT&gt; Tc- adduct. After purification and recovery in a physiological solution, the new borane-nitrido Tc-99m derivatives were injected in rats...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain imaging; Nitrido complexes; Technetium-99m.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600005

Alternative chromatographic system for the quality control of lipophilic technetium-99m radiopharmaceuticals such as [99mTc(MIBI)6]+
Provedor de dados: 56
Autores: Faria,D.P.; Buchpiguel,C.A.; Marques,F.L.N.
Knowledge of the radiochemical purity of radiopharmaceuticals is mandatory and can be evaluated by several methods and techniques. Planar chromatography is the technique normally employed in nuclear medicine since it is simple, rapid and usually of low cost. There is no standard system for the chromatographic technique, but price, separation efficiency and short time for execution must be considered. We have studied an alternative system using common chromatographic stationary phase and alcohol or alcohol:chloroform mixtures as the mobile phase, using the lipophilic radiopharmaceutical [99mTc(MIBI)6]+ as a model. Whatman 1 modified phase paper and absolute ethanol, Whatman 1 paper and methanol:chloroform (25:75), Whatman 3MM paper and ethanol:chloroform...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiopharmaceuticals; Technetium-99m; Quality control; Chromatography.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000007

Evaluation of an anti-carcinoembryonic monoclonal antibody suitable for immunoscintigraphy
Provedor de dados: 56
Autores: Moraes,D.W.; Lima,E.N.P.; Prado,I.B.; Carneiro,C.R.W.
An anti-carcinoembryonic antigen (CEA) monoclonal antibody (mAb 6D1.1) was evaluated in vitro and in vivo to determine its suitability as a tracer for immunoscintigraphy of colorectal carcinomas. Determination of mAb affinity for CEA showed a constant of association of 0.63 ± 0.11 x 109 M-1. Binding of technetium-99m (99mTc)-6D1.1, labeled by a direct method, to human cultured lineages was highly specific. Binding to only CEA-positive LS-174T cells resulted in a saturable curve inhibited by pre-incubation with unlabeled mAb. No binding at all was observed for the human lineages MeWo (melanoma) or ZR75-30 (breast carcinoma), neither of them expressing CEA cells. Intravenous injection of 99mTc-6D1.1 into nude mice xenografted with human LS-174T tumors...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monoclonal antibody; Carcinoembryonic antigen; Colorectal carcinoma; Immunoscintigraphy; Technetium-99m.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800006

Protection of plasmid DNA by a Ginkgo biloba extract from the effects of stannous chloride and the action on the labeling of blood elements with technetium-99m
Provedor de dados: 56
Autores: Moreno,S.R.F.; Freitas,R.S.; Rocha,E.K.; Lima-Filho,G.L.; Bernardo-Filho,M.
Ginkgo biloba extract (EGb) is a phytotherapeutic agent used for the treatment of ischemic and neurological disorders. Because the action of this important extract is not fully known, assays using different biological systems need to be performed. Red blood cells (RBC) are labeled with technetium-99m (Tc-99m) and used in nuclear medicine. The labeling depends on a reducing agent, usually stannous chloride (SnCl2). We assessed the effect of different concentrations of EGb on the labeling of blood constituents with Tc-99m, as sodium pertechnetate (3.7 MBq), and on the mobility of a plasmid DNA treated with SnCl2 (1.2 µg/ml) at room temperature. Blood was incubated with EGb before the addition of SnCl2 and Tc-99m. Plasma (P) and RBC were separated and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Ginkgo biloba extract; Blood element labeling; Technetium-99m; Stannous chloride; Plasmid DNA.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200015

Radiolabeling of cidofovir with technetium-99m and biodistribution studies
Provedor de dados: 60
Autores: Arribada,Raquel Gregorio; Pereira,Nara Caroline; Cardoso,Valbert Nascimento; Cunha Júnior,Armando da Silva; Barros,André Luís Branco de.
Radiolabeling cidofovir with technetium-99m (99mTc-CDV) is an innovative procedure that enables real-time monitoring of the drug. Essays were performed in vitro, showing high radiolabel stability within 24 h. Blood clearance, biodistribution studies, and scintigraphic images were performed in healthy mice in order to evaluate the profile of the drug in vivo. 99mTc-CDV showed biphasic blood circulation time and significant kidney uptake, indicating that 99mTc-CDV is preferentially eliminated by the renal route. Bones also showed important uptake throughout the experiment. In summary, cidofovir was successfully labeled with technetium-99m and might be used in further studies to track the drug.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cidofovir; Radiotracer; Technetium-99m; Biodistribution; Scintigraphic imaging.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100570