Consulta: subjectFacets:"Test de cytodétection"
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Data/hora: 01/06/2024 07:45:11

Etude de pré-validation du test de cytodétection des toxines diarrhéiques (acide okadaïque) dans les coquillages contaminés
Provedor de dados: 5
Autores: Le Gal, Dominique; Marcaillou-le Baut, Claire.
A cytodetection test of diarrhoeic phycotoxins was developped by both IFREMER and Nantes University. Its principle is based on cell morphological changes of KB strain, induced by inhibition of protein-phosphatases involved in cytoskeleton building. This test has been shown sensitive, fast and specific for protein phosphatase inhibitors like diarrhoeic toxins (specialy okadaic acid in France). It shows a high repeatability and reproducibility with pure toxin for the cell strain is highly stable (minimal active concentration or MAC = 125 ng OA.ml-1) In order to evaluate the efficiency oftransfering.the method to coastallaboratories, the assay was subjected to a prevalidation study. The sampling, consisting of five species of shellfish, was carried out during...
Tipo: Text
Palavras-chave: Phycotoxine; Acide okadaïque; Test de cytodétection; Prévalidation; Phycotoxin; Okadaïc acid; Cytodetection assay; Prevalidation.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/00138/24909/23002.pdf