Consulta: subjectFacets:"Toxoplasma gondii"
Registros recuperados: 104
Data/hora: 11/06/2024 06:38:04

Environmental risk factors for canine toxoplasmosis in a deprived district of Botucatu, SP, Brazil
Provedor de dados: 80
Autores: Camossi,LG; Faccioli,PY; Menozzi,BD; Daher,SR; Langoni,H.
Toxoplasmosis is a worldwide zoonosis caused by Toxoplasma gondii that can infect a large variety of animals, including humans. The present study aimed to evaluate the frequency of anti-T.gondii antibodies in dogs from a peripheral district of Botucatu and to establish the association among some epidemiological variables in order to evaluate risk factors for toxoplasmosis infection. Serum samples from dogs were screened using an indirect fluorescent antibody (IFA) test. Anti-T.gondii antibody prevalence was 56%. The highest titer was 1024 (1.79%) and the most frequent titers were 16 (57.14%) and 64 (33.93%). The chi-square (X²) test revealed significant association among variables such as dog access to street, ingestion of raw meat and presence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Dogs; IFA; Risk factors.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300006

Prevalence and risk factors for human toxoplasmosis in a rural community
Provedor de dados: 80
Autores: Marques,JM; da Silva,DV; Correia,NAB; Velásquez,G; da Silva,RC; Langoni,H; da Silva,AV.
Toxoplasma gondii infection may lead to important pathological questions, especially in rural areas, where several sources of infection exist. Therefore, it is important to determine risk factors in order to establish adequate prophylactic measures. The present study aimed to assess the prevalence and risk factors involved in human toxoplasmosis infection in a rural community, in Eldorado, Mato Grosso do Sul State, Brazil. This community was composed of 185 farms - with 671 inhabitants - from which 20 were randomly chosen. In these farms, blood samples were collected from rural workers, who also answered a risk factor questionnaire. Serum samples were analyzed by means of direct agglutination test for the detection of anti-Toxoplasma gondii antibodies....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Prevalence; Risk factors; Rural community.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400010

Cytomegalovirus and Toxoplasma gondii seroprevalence in a Brazilian liver transplant waiting
Provedor de dados: 80
Autores: Almeida,R. A. M. B.; Silva,G. F.; Llanos,J. C.; Winckler,C. C.; Gomes,M. R. B.; Biagioni,D. S.; Paula,R. C. C.; Meira,D. A.; Bakonyi,A.
Cytomegalovirus (CMV) disease is a major cause of morbidity and mortality in solid organ transplantation. Disseminated toxoplasmosis after liver transplantation is a rare but fatal event. Serologic screening of the donor and the recipient is essential to prophylactic management, early diagnosis and therapeutic strategies to minimize the consequences of these infections. The aim of the present study was to determine the seroprevalence of CMV and Toxoplasma gondii (TG) in a Brazilian liver transplant waiting list (LTWL). Serological data were collected from 44 candidates on the LTWL between May 2003 and November 2004. Serological investigation of antibodies IgM and IgG against CMV (anti-CMV) and TG (anti-T. gondii) was performed using fluorometry commercial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytomegalovirus; Liver transplantation; Toxoplasmosis; Toxoplasma gondii; Seroprevalence; Waiting list.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400016

Measurement of IL-10 serum levels in BALB/c mice treated with beta-1,3 polyglucose or sulfadiazine and acutely infected by Toxoplasma gondii
Provedor de dados: 80
Autores: Picka,M. C. M.; Calvi,S. A.; Lima,C. R. G.; Santos,I. A. T.; Marcondes-Machado,J.
Acute infection by Toxoplasma gondii leads to suppression of cell-mediated immunity, facilitating chronic infection. One of the causes of immunosuppression is Interleukin-10 (IL-10) production. Glucan is used to stimulate phagocytosis. Our objective was to study IL-10 induction in male BALB/c mice with acute T. gondii BTU-2 strain infection, glucan immunostimulation, and sulfadiazine treatment. Animals were distributed into 7 groups: G1: infected with T. gondii; G2: infected with T. gondii and treated with sulfadiazine; G3: infected with T. gondii and immunostimulated with glucan; G4: infected with T. gondii, immunostimulated with glucan, and treated with sulfadiazine; G5: imunostimulated with glucan; G6: treated with saline; and G7: treated with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Interleukin-10; Glucan; Immune response; Sulfadiazine.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400012

Detection of Toxoplasma gondii in the milk of experimentally infected Wistar female rats
Provedor de dados: 80
Autores: Costa,VM; Langoni,H.
The milk of experimentally infected rats was investigated for the presence and possible transmission of Toxoplasma gondii. Wistar (Rattus norvegicus) female rats were divided into three groups and orally inoculated with bradyzoites. Polymerase chain reaction (PCR) was employed to detect the parasite in the milk. Transmission to the offspring was verified by indirect immunofluorescence antibody test (IFAT), modified agglutination test (MAT), bioassay tests and PCR. Rat milk samples were PCR-positive, pups were serum-reactive to T. gondii and tissue samples also presented positive DNA results through PCR.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Parasite in milk; Female rats; PCR; Bioassay.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200016

Serological profile of anti-Toxoplasma gondii antibodies in apparently healthy dogs of the city of Botucatu, São Paulo State, Brazil
Provedor de dados: 80
Autores: Langoni,H.; Modolo,J. R.; Pezerico,S. B.; Silva,R. C.; Castro,A. P. B.; Silva,A. V. da; Padovani,C. R.
Toxoplasmosis is a zoonosis caused by Toxoplasma gondii, a coccidian protozoan of worldwide distribution. The seroprevalence in canine population can be an alternative for measuring T. gondii urban spreading. A total of 780 blood samples from dogs were collected, during the yearly anti-rabies campaign, carried out by the Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Husbandry (FMVZ), São Paulo State University, UNESP, together with the county health authorities, in August 1999. Using Indirect Fluorescent Antibody Test (IFAT) for detecting antibodies anti-T. gondii in the sera samples, we observed that 258 dogs (33.1%) were positive. The associations between the serological results and the epidemiological...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Toxoplasma gondii; Dog; Serology.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000100012

Seroepidemiological analysis of toxoplasmosis in college students
Provedor de dados: 80
Autores: Rodrigues,Jaqueline Polizeli; Frei,Fernando; Navarro,Italmar Teodorico; Silva,Luciana Pereira; Marcelino,Monica Yonashiro; Andrade-Junior,Heitor Franco de; Faria,Carolina Arruda de; Santos,Marislene; Ribeiro-Paes,João Tadeu.
Background Toxoplasmosis is a zoonosis caused by an obligate intracellular parasite, Toxoplasma gondii, which affects warm-blooded animals including humans. Its prevalence rates usually vary in different regions of the planet. Methods In this study, an analysis of the seroprevalence of toxoplasmosis among Brazilian students was proposed by means of IgG specific antibodies detection. The presence of anti-Toxoplasma gondiiantibodies by indirect fluorescent antibody test (IFAT) was also evaluated in order to compare it with enzyme-linked immunosorbent assay (ELISA) and to assess the use of 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and o-phenylenediamine dihydrochloride chromogens. Results The IFAT method showed a seroprevalence of 22.3%. These...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Toxoplasmosis; Enzyme-linked immunosorbent assay; Indirect fluorescent antibody test; Seroprevalence; Epidemiology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100311

Detection of antibodies to Toxoplasma gondii in wild animals in Brazil
Provedor de dados: 80
Autores: Silva,Rodrigo Costa da; Machado,Gustavo Puglia; Cruvinel,Tatiane Morosini de Andrade; Cruvinel,Ciro Alexandre; Langoni,Helio.
Background Toxoplasmosis is a worldwide zoonosis caused by an obligate intracellular protozoan parasite,Toxoplasma gondii, that affects all warm-blooded animals, including wild animals. The increased number of cases of parasitic infections is mainly due to the destruction of environmental conservation areas, which is driving wild animals out of their habitats and towards urban areas. In this study, the occurrence of T. gondii infection was investigated by the modified agglutination test (MAT) in 26 different species of run over and injured wild animals that were treated at a Brazilian university veterinary hospital, from June 2007 to August 2008.Findings Of the studied animals, six (23.1%; CI95% 11.1-42.2%) had T. gondii antibodies, with titers equal to 10...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Natural infection; Antibodies; Wild animals; MAT.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200603

Toxoplasmosis, leptospirosis and brucellosis in stray dogs housed at the shelter in Umuarama municipality, Paraná, Brazil
Provedor de dados: 80
Autores: Dreer,Márcia Küster de Paula; Gonçalves,Daniela Dib; Caetano,Isabel Cristina da Silva; Gerônimo,Edson; Menegas,Paulo Henrique; Bergo,Danilo; Lopes-Mori,Fabiana Maria Ruiz; Benitez,Aline; Freitas,Julio Cesar de; Evers,Fernanda; Navarro,Italmar Teodorico; Martins,Lisiane de Almeida.
Background : Leptospirosis, toxoplasmosis and brucellosis are diseases with worldwide distribution. Among stray dogs, these zoonoses are facilitated by direct contact with other animal species, by the habit of scavenging garbage and hunting in search of food, drinking standing water, smelling other animals' urine, licking female genitalia and the sexual act itself. The objective of this study was to detect antibodies anti-Toxoplasma gondii, anti-Leptospira spp., anti-Brucella canis and anti-Brucella abortus in stray dogs housed in shelters at Umuarama city, Paraná, Brazil. In order to detect toxoplasmosis, indirect immunofluorescence assay (IFA) was performed, agglutination microscopic (MAT) test for leptospirosis and agar gel immunodiffusion (AGID) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira spp; Brucella abortus; Brucella canis; Toxoplasma gondii; Zoonosis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100313

Non-association between anti-Toxoplasma gondii antibodies and ABO blood group system
Provedor de dados: 80
Autores: Rodrigues,ACF; Uezato,S; Vono,MB; Pandossio,T; Spegiorin,LCJF; Oliani,AH; Vaz Oliani,DCM; Brandão de Mattos,CC; de Mattos,LC.
Toxoplasma gondii infects humans through the gastrointestinal tract (GIT), which elicits humoral immune response with specific antibodies. The expression of the ABO blood group glycoconjugates also occurs in this same system and may influence the human susceptibility of infection by T. gondii. The aim of the present study was to investigate the association between ABO blood group phenotypes and the presence of anti-T. gondii antibodies. Data - including age, results of serology tests for T. gondii infection and ABO blood group phenotypes - were assembled from the medical records of 1,006 pregnant women attended in the Base Hospital of the Medical School of São José do Rio Preto, Brazil, between 2001 and 2004. The chi-square test was used to compare the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human blood groups; Toxoplasma gondii; Toxoplasmosis; ABO blood group; Pregnant women.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200009

Hypertrophy of NADH-diaphorase positive myenteric neurons in rat jejunum after acute infection caused by Toxoplasma gondii
Provedor de dados: 80
Autores: Pereira,LS; Silva,AV; Araújo,EJA; Sant'Ana,DMG.
Toxoplasmosis, a globally distributed feline-associated zoonosis caused by the protozoan Toxoplasma gondii, affects birds and mammals, including humans. This study assesses the consequences of acute T. gondii infection for NADH-diaphorase positive myenteric neurons in rat jejunum. Ten male Wistar rats (Rattus norvegicus) were divided into two groups: G1 (n = 5) and G2 (n = 5). Animals from G2 were orally inoculated with 500 genotype III (M7741) T. gondii oocysts. Twenty-four hours after inoculation, the animals were euthanized and had their jejuna removed, through laparotomy, and measured (length and width) to calculate their areas. Intestinal segments were submitted to NADH-diaphorase histochemistry to evidence the most metabolically active subpopulation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Jejunum; Myenteric plexus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200011

Serological survey of Toxoplasma gondii infection amoug Urban (Manila) and Suburban (Dasmariñas,Cavite) Residents, Philippines
Provedor de dados: 19
Autores: Salibay, C. C.; Dungca, J. Z.; Claveria, Florencia G.
Serological survey of Toxoplasma gondii infection was carried out in 68 urban (Manila) and 72 suburban (Dasmariñas, Cavite) residents, comprising 69 females and 71 males, age 16-56 years old. Using a survey questionnaire, the name and address, gender, age, weight, height, civil status, cat exposure, and additional information like history of pregnancy and current reproductive status of females were obtained. The presence of anti-T.gondii antibodies was confirmed using the Toxocell latex agglutination test. The pooled population revealed 38 (27.1%) seropositive (sero+) cases, with higher infection rate among the suburban (30.6%) and female (32.0%) respondents. Differences in percent infection between the study groups, sexes and respondents with normal,...
Palavras-chave: Serological assay; Toxoplasma gondii; Urban and suburban dwellers; Philippines.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1835

Apical membrane antigen 1 is a cross-reactive antigen between Neospora caninum and Toxoplasma gondii, and the anti-NcAMA1 antibody inhibits host cell invasion by both parasites
Provedor de dados: 19
Autores: Zhang, Houshuang; Compaore, Muller K.A.; Lee, Eung-goo; Liao, Min; Zhang, Guohong; Sugimoto, Chihiro; Fujisaki, Kozo; Nishikawa, Yoshifumi; Xuan, Xuenan.
The cross-reactive antigens of Neospora caninum and Toxoplasma gondii are important in the exploration to determine the common mechanisms of parasite-host interaction. In this study, a gene encoding N. caninum apical membrane antigen 1 (NcAMA1) was identified by immunoscreening of a N. caninum tachyzoite cDNA expression library with antisera. from mice immunized with recombinant T gondii apical membrane antigen 1 (TgAMA 1). NcAMA1 was encoded by an open reading frame of 1695 bp, which encoded a protein of 564 amino acids. The single-copy NcAMA1 gene was interrupted by seven introns. NcAMA1 showed 73.6% amino acid identity to TgAMA1. Mouse polyclonal antibodies raised against the recombinant NcAMA1 (rNcAMA1) recognized a 69-kDa native parasite protein by...
Palavras-chave: Neospora caninum; Toxoplasma gondii; Apical membrane antigen 1; Cross-reactive; Invasion.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1034

Characterization of a novel thrombospondin-related protein in Toxoplasma gondii
Provedor de dados: 19
Autores: Kawase, Osamu; Nishikawa, Yoshifumi; Bannai, Hiroshi; Matsuo, Tomohide; Xuan, Xuenan.
Toxoplasma gondii is an obligate intracellular protozoan parasite that invades a wide range of host cells. Upon encountering host cells, the parasite releases a large variety of proteins from secretory organelles, such as micronemes, rhoptries and dense granules. The secretion of microneme protein is essential for parasite invasion. We found that a secreted protein with an altered thrombospondin repeat of Toxoplasma gondii (TgSPATR) was a novel microneme protein, which was different from known microneme proteins that carry thrombospondin repeat domains. TgSPATR was secreted in response to an intra-parasitic elevation of Ca2+ and probably secreted during early stages of parasite invasion. Thus, we suggested that TgSPATR, new member of microneme secretory...
Palavras-chave: Ca2+-dependent secretion; Microneme protein; Secreted protein with an alteredthrombospondin repeat; Toxoplasma gondii.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2819

Enzyme-linked immunosorbent assays using recombinant TgSAG2 and NcSAG1 to detect Toxoplasma gondii and Neospora caninum-specific antibodies in domestic animals in Turkey
Provedor de dados: 19
Autores: Zhou, Mo; Cao, Shinuo; Sevinc, Ferda; Sevinc, Mutlu; Ceylan, Onur; Liu, Mingming; Wang, Guanbo; Moumouni, Paul Franck Adjou; Jirapattharasate, Charoonluk; Suzuki, Hiroshi; Nishikawa, Yoshifumi; Xuan, Xuenan.
Considering the scarce information on occurrences of Toxoplasma gondii and Neospora caninum in domestic animals from Turkey, the aim of this study was to investigate the seroprevalence of these parasite infections in cattle, horses, sheep, goats and dogs in Turkey. The specific antibodies against T gondii and N. caninum were detected by iELISAs based on the recombinant TgSAG2 or NcSAG1 in a total of 2,039 serum samples from eleven provinces. The seroprevalence of T. gondii infections was 46.3%, 4.0%, 20.0%, 12.9% and 19.8%, that of N. caninum infections was 0.3%, 7.4%, 2.1%, 3.2% and 16.6% in the horses, cattle, sheep, goats and dogs, respectively. These results indicated that T gondii and N. caninum infections are prevalent in Turkish domestic animals.
Palavras-chave: ELISA; Neospora caninum; Toxoplasma gondii; Turkey.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4422

Toxoplasma gondii: Antibody-independent Binding of Human Complement Subcomponent Clq to the Parasite
Provedor de dados: 19
Autores: Omata, Yoshitaka; Yonemasu, Kunio; Sasaki, Takako; Saito, Atsushi; Nakabayashi, Toshio; Suzuki, Naoyoshi.
Palavras-chave: Antibody-independent binding; Clq; Toxoplasma gondii.
Ano: 1992
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/175

Role of a 65-kDa Heat Shock Protein in Protective Immunity against Toxoplasma Infection
Provedor de dados: 19
Autores: Himeno, Kunisuke; Nagasawa, Hideyuki; Hisaeda, Hajime.
Palavras-chave: Toxoplasma gondii; HSP65; Protective immunity.
Ano: 1993
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/189

Characterization and Check of Identity of Different Strains of Toxoplasma gondii by Enzymatic Profiling
Provedor de dados: 19
Autores: Hassl, Andreas; Manafi, Mohammed; Sommer, Regina; Kundi, M.; Aspöｃｋ, Horst.
Palavras-chave: Toxoplasma gondii; Strain differentiation; Enzyme profile; Zymogram analysis; API enzyme research kit.
Ano: 1994
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/204

Isolation of the Plastid DNA of Toxoplasma gondii
Provedor de dados: 19
Autores: Munro, Sian C.; Johnson, Alan M.
Palavras-chave: Toxoplasma gondii; Plastid; Extrachromosomal DNA; Isolation techniques.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/326

Host Cholesterol Synthesis Contributes to Growth of Intracellular Toxoplasma gondii in Macrophages
Provedor de dados: 19
Autores: NISHIKAWA, Yoshifumi; IBRAHIM, Hany M.; KAMEYAMA, Kyohko; SHIGA, Ikumi; HIASA, Jun; XUAN, Xuenan; 西川, 義文; 玄, 学南.
The intracellular protozoan Toxoplasma gondii lacks the ability to synthesize sterol and scavenges cholesterol from the lowdensity lipoprotein receptor (LDLR) pathway of its host to facilitate replication. Sterol biosynthesis inhibitors, however, have a demonstrated anti-Toxoplasma effect. In this study, we examined the host mevalonate pathway as a novel source of cholesterol for T. gondii and its effects on parasite growth in macrophages. Parasite growth did not significantly change in the absence of LDLR or when LDL was exogenously supplemented. Lovastatin and compactin, both inhibitors of hydroxymethylglutaryl-CoA (HMG-CoA) reductase in the mevalonate pathway, significantly inhibited T. gondii growth in both wild-type and LDLR-knockout macrophages....
Palavras-chave: Cholesterol; Lipid metabolism; Macrophage; Parasitology; Toxoplasma gondii.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3583

Production of Transgenic Mice Carrying p30 Gene Encoding Major Surface Antigen of Toxoplasma gondii
Provedor de dados: 19
Autores: Seng, Seyha; Maki, Yoshiyuki; Yokoyama, Minesuke; Inoue, Noboru; Igarashi, Ikuo; Nagasawa, Hideyuki; Horiuchi, Motohiro; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi; Toyoda, Yutaka; 井上, 昇; 五十嵐, 郁男; 長澤, 秀行.
Palavras-chave: Toxoplasma gondii; SAG-1 (p30); Transgenic mice.
Ano: 1996
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/254

Characterization of a Toxoplasma gondii calcium calmodulin-dependent protein kinase homolog
Provedor de dados: 19
Autores: Kato, Kentaro; Sugi, Tatsuki; Takemae, Hitoshi; Takano, Ryo; Gong, Haiyan; Ishiwa, Akiko; Horimoto, Taisuke; Akashi, Hiroomi.
Background: Toxoplasma gondii is an obligate intracellular parasite of the phylum Apicomplexa and a major pathogen of animals and immunocompromised humans, in whom it causes encephalitis. Understanding the mechanism of tachyzoite invasion is important for the discovery of new drug targets and may serve as a model for the study of other apicomplexan parasites. We previously showed that Plasmodium falciparum expresses a homolog of human calcium calmodulin-dependent protein kinase (CaMK) that is important for host cell invasion. In this study, to identify novel targets for the treatment of Toxoplasma gondii infection (another apicomplexan parasite), we sought to identify a CaMK-like protein in the T. gondii genome and to characterize its role in the...
Palavras-chave: Calcium calmodulin-dependent protein kinase homolog; GAP45; Phosphorylation; T. gondii CaMK-related kinase; Toxoplasma gondii.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4447

The impact of the host CCR5, TLR2 and TLR11 on production of nitric oxide, IL-6, IL-12 and growth of Toxoplasma gondii
Provedor de dados: 19
Autores: Ibrahim, H. M.; Nishikawa, Y.
The current study investigated the impact of the CCR5, TLR2 and TLR11 on production of nitric oxide (NO), IL-6 and IL-12 and growth of T. gondii, high virulent RH strain and avirulent PLK strain. All examined knockout macrophages infected with PLK strain produced significant lower levels of IL-12. On the other hand, TLR2 and TLR11 macrophages infected with RH strain produced significantly reduced levels of IL-12 as compared to wild-type macrophages. TLR2 and TLR11 macrophages infected with PLK strain significantly inhibited the production of IL-6 when compared to wild-type macrophages. Interestingly, significant reduction in the IL-6 and NO production was observed in TLR2 macrophages infected with PLK strain as compared to wild-type macrophages or to the...
Palavras-chave: Toxoplasma gondii; CCR5; TLR2; TLR11.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4495

Cloning and characterization of aspartic protease 3 of Toxoplasma gondii
Provedor de dados: 19
Autores: Kamyingkird, K.; Goo, Y.K.; Cao, S.; Adjou Moumouni, P.K.; Aboge, G.O.; Yamagishi, J.; Terkawi, M.A.; Masatani, T.; Yu, L.; Nishikawa, N.; Xuan, X.
Palavras-chave: Aspartic protease; Toxoplasma gondii.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4093

Structural and Molecular Differences in the Host Parasite Relationship between the Enteric (Coccidian) and Exoenteric Forms of Toxoplasma gondii
Provedor de dados: 19
Autores: Ferguson, D. J. P.
Palavras-chave: Toxoplasma gondii; Host/parasite relationship; Immunocytochemistry; Electron microscopy.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/328

Evaluation of novel oocyst wall protein candidates of Toxoplasma gondii
Provedor de dados: 19
Autores: Salman, Doaa; Okuda, Liria Hiromi; Ueno, Akio; Dautu, George; Zhang, Feifei; Igarashi, Makoto.
Oocyst stage of Toxoplasma gondii is characterized by a durable wall that confers a strong protection to this protozoan parasite in face of harsh environmental conditions. Thus, it is considered the key for transmission of T. gondii. Analysis of oocyst wall composition is mandatory therefore; the aim of this study was to identify novel T. gondii oocyst wall proteins and test their use in detection of these oocysts in environmental samples. Five candidates of novel T. gondii oocyst wall proteins (TgOWPs) were identified and named TgOWP8 through TgOWP12. Recombinant protein of TgOWP8 was expressed in E. coli using glutathione S-transferase as fusion protein. Polyclonal antibody was produced and validated by indirect immunofluorescence antibody assay (IFA)....
Palavras-chave: T. gondii oocyst wall protein; Toxoplasma gondii.
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4541

Expression of P30, The Major Surface Antigen of Toxoplasma gondii in Baculovirus-insect System and The Evaluation of Immune Response Induced by P30
Provedor de dados: 19
Autores: Chen, Xiao-Guang; Ma, Xin; Wu, Kun; Peng, Hong-Juan; Shen, Shu-Man; Liu, Guo-Zhang; Chan, Siu-Ming.
Palavras-chave: Toxoplasma gondii; Major surface antigen; Baculovirus; Expression system; Immune.
Ano: 1998
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/275

Dextran sulfate inhibits acute Toxoplama gondii infection in pigs
Provedor de dados: 19
Autores: Kato, Kentaro; Murata, Yuho; Horiuchi, Noriyuki; Inomata, Atsuko; Terkawi, Mohamad Alaa; Ishiwa, Akiko; Ogawa, Yohsuke; Fukumoto, Shinya; Matsuhisa, Fumikazu; Koyama, Kenji.
Background: Toxoplasma gondii is a highly prevalent protozoan that can infect all warm-blooded animals, including humans. Its definitive hosts are Felidae and its intermediate hosts include various other mammals and birds, including pigs. It is found in the meat of livestock which is a major source of human infection. Hence the control of toxoplasmosis in pigs is important for public health. We previously showed that dextran sulfate (DS), especially DS10 (dextran sulfate MW 10 kDa), is effective against T. gondii infection both in vitro and in mice. In this study, we asked whether DS affects T. gondii infection of pigs, one of the main animal sources of toxoplasmosis transmission to humans. Methods: Fourteen-day-old male pigs (n = 10) were infected with T....
Palavras-chave: Dextran sulfate; Intermediate host; Pig; Public health; Toxoplasma gondii.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4446

原虫感染症の発症予防と早期診断法の分子免疫学的開発研究
Provedor de dados: 19
Autores: 鈴木, 直義.
平成5年度科学研究費補助金(試験研究B)研究成果報告書
Palavras-chave: Cat; Isospora felis; Toxoplasma gondii.
Ano: 1994
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1758

Expression of 65 000 MW Heat-shock Protein in SCID Mice Infected with Toxoplasma gondii after Transplantation of Mouse Fetal Thymus
Provedor de dados: 19
Autores: Nagasawa, Hideyuki; Hisaeda, Hajime; Ishikawa, Hiroyuki; Maekawa, Yoichi; Sakai, Tohru; Himeno, Kunisuke; 長澤, 秀行.
Palavras-chave: Heat shock protein; HSP65; Toxoplasma gondii; Protozoa; SCID mice; Protective immunity.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/229

Serological Status to Natural Toxoplasma gondii Infection in Mixed Flocks of Sheep and Goats in Bangladesh
Provedor de dados: 19
Autores: Samad, Md Abdus; Rahman, K. M. B.; Basher, S. A.
Palavras-chave: Toxoplasma gondii; Latex agglutination; Seropositive.
Ano: 1993
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/181

Involvement of Host Defense Mechanisms against Toxoplasma gondii Infection in Anhedonic and Despair-Like Behaviors in Mice
Provedor de dados: 19
Autores: Mahmoud, Motamed Elsayed; Fereig, Ragab; Nishikawa, Yoshifumi.
Toxoplasma gondii is a pathogen relevant to psychiatric disorders. We recently showed that reactivation of chronic T. gondii infection induced depression-like behaviors in mice. Furthermore, it has been hypothesized that depression-like behaviors are mediated via a host defense mechanism against invading pathogens; proximate mechanisms of this behavioral hypothesis remain unclear. In the present study, we investigate the contribution of indoleamine 2,3-dioxygenase (IDO), inflammation, and interferon gamma (IFN-gamma) to anhedonic and despair-related behaviors in T. gondii-infected mice by using sucrose preference and forced-swim tests, respectively. First, we confirmed that BALB/c mice exhibited both sickness and depression-like behaviors during acute...
Palavras-chave: Toxoplasma gondii; 1-methyl tryptophan; Minocycline; Interferon gamma; Sickness behavior; Anhedonic behavior; Despair-like behavior.
Ano: 2017
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4507

Synthetic retinoid Am80 controls growth of intracellular Toxoplasma by inhibiting acquisition and synthesis of cholesterol in macrophages
Provedor de dados: 19
Autores: Ihara, F.; Nshikawa, Y.
Palavras-chave: Toxoplasma gondii; Cholesterol; Triacylglycerols.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4091

Toxoplasma gondii: DNA vaccination with genes encoding antigens MIC2, M2AP, AMA1 and BAG1 and evaluation of their immunogenic potential
Provedor de dados: 19
Autores: Dautu, George; Munyaka, Biscah; Carmen, Gabriella; Zhang, Guohong; Omata, Yoshitaka; Xuan, Xuenan; Igarashi, Makoto.
A combination of antigenic regions of microneme proteins have been previously reported as being protective against chronic toxoplasmosis. In this work, we evaluated immune responses induced by immunizing BALB/c and C57BL/6 mice intradermally with plasmid DNA encoding the protein sequences of Toxoplasma gondii AMA1, MIC2, M2AP and BAG1. Mice immunized with the AMA1 gene developed high levels of serum IgG2a and c antibodies as well as cellular immune responses associated with IFN-γ synthesis suggesting a modulated Th1 type of response. Immunization with the AMA1 gene resulted in a partial but significant protection against the acute phase of toxoplasmosis compared to MIC2, M2AP and BAG1 genes. Therefore, the AMA1 gene appears to generate a strong specific...
Palavras-chave: Toxoplasma gondii; DNA; Deoxyribonucleic acid; DNA immunization; MIC2; Microneme protein-2; M2AP; MIC2 associated protein; AMA1; Apical membrane antigen-1; BAG1; Bradyzoite antigen-1; IgG; Immunoglobulin G.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1031

Peroxiredoxin 3 promotes IL-12 production from macrophages and partially protects mice against infection with Toxoplasma gondii
Provedor de dados: 19
Autores: Fereig, Ragab M.; Nishikawa, Yoshifumi.
Toxoplasmosis remains a life-threatening infection of humans and various domestic and wild animals worldwide. It is caused by the obligatory intracellular protozoan parasite Toxoplasma gondii. Peroxiredoxins (Prxs) are a family of antioxidant enzymes that protect cells from oxidative stress from hydroperoxides. In the recent years, several studies have reported the potential use of T. gondii-derived enzymes in triggering protective immunity against T. gondii infection. Therefore, this study was conducted to investigate the immunogenicity and protective efficacy of TgPrx3. In vitro stimulation of peritoneal macrophages with recombinant TgPrx3 protein fused to glutathione-S transferase (TgPrx3-GST) enhanced IL-12p40 production, indicating the...
Palavras-chave: Toxoplasma gondii; Peroxiredoxin 3; Vaccine; Immunization.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4569

Seroprevalences of Toxoplasma gondii and Neospora caninum in Pet Rabbits in Japan
Provedor de dados: 19
Autores: SALMAN, Doaa; OOHASHI, Eiji; MOHAMED, Adel Elsayed Ahmed; ABD EL-MOTTELIB, Abd El-Raheem; OKADA, Tadashi; IGARASHI, Makoto; 五十嵐, 慎.
The potential contamination of Toxoplasma gondii and Neospora caninum oocysts in the human environment is a concern from the public health viewpoint. However, estimation of their seroprevalences in humans cannot be performed in a manner that distinguishes between oocysts and tissue cysts as a source of infection. Rabbits are considered popular pet animals in Japan that can acquire natural infections by the aforementioned parasites only through the ingestion of oocysts. Therefore, this study was conducted to estimate the seroprevalences of T. gondii and N. caninum in pet rabbits in Japan as an indicator of the possible oocyst contamination in the environment surrounding human beings. Serum samples of 337 rabbits were examined by different serological...
Palavras-chave: Neospora caninum; Rabbit; Seroprevalence; Toxoplasma gondii.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3936

The Influence of the regulation of Toxoplasma gondii TgMIC2 transgene on host cell infection
Provedor de dados: 19
Autores: Buates, S.; Xuan, Xuenan; Igarashi, Makoto; Sugimoto, Chihiro; Inoue, Noboru; 玄, 学南; 五十嵐, 慎; 井上, 昇.
Toxoplasma gondii microneme protein 2 (TgMIC2), an apically stored adhesin, was shown to be a key participant involved in the initial attachment and invasion to a host cell. In this study, we had established the clonal line of T. gondii tachyzoites which over-expressed TgMIC2 using the tetracycline repressor (TetR)-based inducible gene expression system. The TgMIC2 over-expression significantly reduced tachyzoite propagation in vitro (p &lt; 0.002) and significantly reduced the parasite virulence in mice (p = 0.002). The over-expression of exogenous TgMIC2 caused the increase of mRNA expression level of endogenous TgMIC2. Wild type parasites mainly expressed TgMIC2115 protein, the microneme store and the surface form of TgMIC2. Over-expression of TgMIC2...
Palavras-chave: Toxoplasma gondii; TgMIC2; Over-expression.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2236

Serodiagnosis of Toxoplasma gondii in ducks from Behera Governorate, Egypt
Provedor de dados: 19
Autores: AbouLaila, M; El-Bahy, N; Hilali, M; Yokoyama, N; Igarashi, I; 横山, 直明; 五十嵐, 郁男.
Toxoplasma gondii is an important zoonotic parasite. The diagnosis of infection in animals is an important tool to prevent human infection. In the present study, Toxoplasma gondii was diagnosed by using the modified agglutination test (MAT) in ducks from Behera Governorate, Egypt. The prevalence of Toxoplasma gondii was 13.9%. The prevalence of Toxoplasma gondii was the highest in the Native breed (17.65%) and in 6-8-months age group (19.4%). End-point titer of 1:25, 1:50, and 1:100 were recorded in 61.9%, 19.05%, and 19.05% of the positive samples, respectively. Duck meat is one source of the Toxoplasma gondii infection for human in Egypt.
Palavras-chave: Toxoplasma gondii; Modified agglutination test (MAT); Ducks; Egypt.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3128

Toxoplasma gondii modulates neutral lipid metabolism in macrophage J774 cells
Provedor de dados: 19
Autores: Kameyama, K; Nishimura, M; Ybañez, R.D.; Hiasa, J; Xuan, X; Nishikawa, Y; 玄, 学南; 西川, 義文.
Palavras-chave: Toxoplasma gondii; Cholesterol; Triacylglycerols; Lipid bodies.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3129

Seroprevalence and epidemiology of Toxoplasma gondii in farm animals in different regions of Egypt
Provedor de dados: 19
Autores: Fereig, Ragab M.; Mahmoud, Hassan Y.A.H.; Mohamed, Samy G.A.; AbouLaila, Mahmoud Rezk; Abdel-Wahab, Azza; Osman, Salama Ahmed; Zidan, Sherif Abdallah; El-Khodary, Sabry A.; Mohamed, Adel Elsayed Ahmed; Nishikawa, Yoshifumi.
Toxoplasmosis is a cosmopolitan protozoan disease that has been recorded in a wide range of vertebrate hosts, including humans. In response to the paucity of available data, this study was undertaken to comprehensively establish the seroprevalence of Toxoplasma gondii among various farm animals in different localities of Egypt. The latex agglutination test and TgGRA7-based enzyme-linked immunosorbent assay were used to screen the investigated animals for anti-T. gondii IgG antibodies. When only samples with simultaneously positive results for both the latex agglutination test and the TgGRA7-based ELISA were considered positive, 174 (26.7%) of 652 serum samples from different animals were seropositive. The prevalence of antibodies according to species was:...
Palavras-chave: Egypt; Seroprevalence; Toxoplasma gondii; Toxoplasmosis.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4545

Serodiagnosis of ovine toxoplasmosis in Mongolia by an enzyme-linked immunosorbent assay with recombinant Toxoplasma gondii matrix antigen 1
Provedor de dados: 19
Autores: Tumurjav, Buyannemekh; Terkawi, Mohamad Alaa; Zhang, Houshuang; Zhang, Guohong; Jia, Honglin; Goo, Youn-Kyoung; Yamagishi, Junya; Nishikawa, Yoshifumi; Igarashi, Ikuo; Sugimoto, Chihiro; Xuan, Xuenan.
Toxoplasma gondii matrix antigen 1 (TgMAG1), known as the 65-kDa protein, which is abundantly expressed in both bradyzoites and tachyzoites, was evaluated as a candidate for the development of a diagnostic reagent for ovine toxoplasmosis. The TgMAG1 gene was expressed in Escherichia coli as a fusion protein with glutathione S-transferase (GST),and the recombinant TgMAG1 (rTgMAG1) was tested in an enzyme-linked immunosorbent assay (ELISA). The ELISA with rTgMAG1 showed a highly specific reaction with sera from mice experimentally infected with T. gondii but not with the closely related Neospora caninum. The antibodies to TgMAG1 were detectable from the acute to the chronic infectious stages in a mouse model. A total of 175 serum samples collected from sheep...
Palavras-chave: ELISA; Mongolia; Sheep; TgMAG1; Toxoplasma gondii.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3018

新規の原虫由来活性ペプチドによる宿主の非特異的免疫賦活性用機序に関する研究
Provedor de dados: 19
Autores: 鈴木, 直義.
平成6年度科学研究費補助金(一般研究A)研究成果報告書03404013
Palavras-chave: Cat; Isopora felis; Toxoplasma gondii.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1759

Characterization of a leucine aminopeptidase from Toxoplasma gondii
Provedor de dados: 19
Autores: Jia, Honglin; Nishikawa, Yoshifumi; Luo, Yuzi; Yamagishi, Junya; Sugimoto, Chihiro; Xuan, Xuenan.
The M17 family leucine aminopeptidase (LAP) hydrolyze amino acids from the N-terminus of peptides. Many LAPs from parasitic protozoa including Plasmmodium, Trypanosoma and Leishmania, have been intensely investigated because of their crucial roles in parasite biology. In this study, a functional recombinant Toxoplasma gondii LAP (rTgLAP) was expressed in Escherichia coli and its enzymatic activity against synthetic substrates for aminopeptidase, as well as the cellular localization was determined. Our results indicated that TgLAP is a functional aminopeptidase in the cytoplasma of T. gondii.
Palavras-chave: Toxoplasma gondii; Leucine aminopeptidase; Enzymatic activity.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2822

Identification and characterization of cross-reactive antigens from Neospora caninum and Toxoplasma gondii
Provedor de dados: 19
Autores: Liao, Min; Xuan, Xuenan; Huang, Xiaohong; Shirafuji, Hiroaki; Fukumoto, Shinya; Hirata, Haruyuki; Suzuki, Hiroshi; Fujisaki, Kozo.
Murine monoclonal antibodies (mAbs) against Neospora caninum tachyzoites were produced to identify the cross-reactive antigens between N. caninum and Toxoplasma gondii. Ten mAbs recognizing cross-reactive antigens of both parasites were obtained and tentatively classified into 6 different groups based on their reactivity patterns in an indirect fluorescent antibody test and Western blot analysis. Three mAbs in group I recognized antigens located on the surface of parasites with molecular masses ranging from 28 to 76 kDa; one mAb in group 2 recognized antigens located on interior organelles of parasites with a molecular mass of 50 kDa; one mAb in group 3 recognized antigens located on interior organelles of parasites with molecular masses of 35 kDa and 14...
Palavras-chave: Neospora caninum; Toxoplasma gondii; Cross-reactive antigen; Protein disulfide isomerase; Heat-shock protein; Ribosomal protein 1.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/816

Identification of ribosomal phosphoprotein P0 of Neospora caninum as a potential common vaccine candidate for the control of both neosporosis and toxoplasmosis
Provedor de dados: 19
Autores: Zhang, Houshuang; Lee, Eung-goo; Liao, Min; Compaore, Muller K.A.; Zhang, Guohong; Kawase, Osamu; Fujisaki, Kozo; Sugimoto, Chihiro; Nishikawa, Yoshifumi; Xuan, Xuenan.
The characterization of the cross-reactive antigens of two closely related apicomplexan parasites, Neospora caninum and Toxoplasma gondii, is important to elucidate the common mechanisms of parasite–host interactions. In this context, a gene encoding N. caninum ribosomal phosphoprotein P0 (NcP0) was identified by immunoscreening of a N. caninum tachyzoite cDNA expression library with antisera from mice immunized with T. gondii tachyzoites. The NcP0 was encoded by a gene with open reading frame of 936 bp, which encoded a protein of 311 amino acids. The NcP0 gene existed as a single copy in the genome and was interrupted by a 432 bp intron. The NcP0 showed 94.5% amino acid identity to T. gondii P0 (TgP0). Anti-recombinant NcP0 (rNcP0) sera recognized a...
Palavras-chave: Neospora caninum; Toxoplasma gondii; Ribosomal phosphoprotein P0; Cross-reactive.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1035

Expression of Babesia gibsoni thrombospondin-related adhesive protein in Toxoplasma gondii and evaluation of its antigenicity and immunogenicity
Provedor de dados: 19
Autores: Kumagai, A.; Zhang, G.; Jia, H.; Nakamura, C.; Zhang, H.; Goo, Y-K.; Aboge, G. A.; Terkawi, M. A.; Zhou, J.; Nishikawa, Y.; Xuan, X.
A gene encoding Babesia gibsoni thrombospondin-related adhesive protein (BgTRAP), known as a vaccine candidate, was stably expressed in Toxoplasma gondii (Tg/BgTRAP). The molecular weight and the antigenic reaction of recombinant BgTRAP expressed by the Tg/BgTRAP were similar to the original ones expressed by B. gibsoni. To evaluate the antigenicity of the recombinant BgTRAP expressed by T. gondii, the lysates of the recombinant parasite tachyzoites were intraperitoneally injected into mice. The serum collected from Tg/BgTRAP-immunized mouse could react to B. gibsoni parasites, while the serum collected from wild-type T. gondii tachyzoites (Tg/wt)-immunized mice did not. These results indicate that T. gondii could provide a new tool to produce foreign...
Palavras-chave: Toxoplasma gondii; Babesia gibsoni; BgTRAP; Expression; Vaccine.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4496

Sequence Analysis of Three Major Antigens (P30, P23 and P22) of Virulent and Avirulent Strains of Toxoplasma gondii
Provedor de dados: 19
Autores: Maki, Yoshiyuki; Seng, Seyha; Kato, Mihoko; Hoshi, Yukihiro; Igarashi, Ikuo; Nagasawa, Hideyuki; Toyoda, Yutaka; Suzuki, Naoyoshi.
Palavras-chave: Toxoplasma gondii; SAG-1 (P30); CDNA.
Ano: 1996
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/250

DENDRITIC CELLS AND HOST RESISTANCE TO TOXOPLASMA GONDII
Provedor de dados: 19
Autores: Makala, Levi H. C.; Nagasawa, Hideyuki.
Palavras-chave: Toxoplasma gondii; IL-12; IFN-γ; Dendritic cells; Host resistance; Mouse.
Ano: 2003
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/634

Detection of Collagen-cross Reactive Antigenic Components in Toxoplasma gondii
Provedor de dados: 19
Autores: Omata, Yoshitaka; Inoue, Noboru; Yonematsu, Kunio; Claveria, Florencia G.; Igarashi, Ikuo; Saito, Atsushi; Suzuki, Naoyoshi; 井上, 昇; 五十嵐, 郁男.
Palavras-chave: Collagen; Cross-reactive antigens; Toxoplasma gondii.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/223

Immunogenicity of Toxoplasma gondii Heat Shock Protein 70
Provedor de dados: 19
Autores: Miller, C. M.; Smith, N. C.; Luton, K.; Dobbin, C. A.; Johnson, Alan M.
Palavras-chave: Toxoplasma gondii; Heat shock protein; Vaccination; Immune response.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/325

Incoordenação de membros posteriores em equinos associados à toxoplasmose - relato de caso
Provedor de dados: 195
Autores: Santos, João Paulo de Almeida Ferreira dos; Nasciutti, Nayara Resende; Oliveira, Patricia Magalhães de; Anjos, Carolina; Garcia, Felipe Gonçalves.
O Toxoplasma gondii é um parasita que tem como hospedeiro definitivo todos os felídeos, e como hospedeiros intermediários os mamíferos, dentre eles o próprio felídeo, o homem e o equino. A contaminação nos herbívoros ocorre pela ingestão de gramíneas e ração contaminadas pelo oocisto. Foi atendido no Hospital Veterinário da Universidade Federal de Uberlândia um equino, macho, quarto de milha, de dois anos e meio de idade, com histórico de fezes pastosas, emagrecimento e incoordenação nos membros posteriores. Ficava com uma égua em um piquete, que morreu dez dias antes com os mesmo sinais clínicos, sendo a alimentação a base de pasto e sal mineral e água em cocho. No hemograma foi constatado anemia, sendo também realizado diagnóstico diferencial para anemia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cavalo; Elisa; Toxoplasma gondii.
Ano: 2015
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/25234

Detection of a high prevalence of antibodies against Toxoplasma gondii in cattle in Northern and Midwestern Brazil
Provedor de dados: 101
Autores: Barbosa da Silva,Jenevaldo; Nunes de Santana Castro,Gustavo; Nunes dos Santos,Priscilla; Henrique da Fonseca,Adivaldo; Henrique da Silva Lima,Danillo; dos Anjos Bomjardim,Henrique; dos Santos Belo Reis,Alessandra; de Oliveira Soares,Susiane; Diomedes Barbosa,José.
The aim of this study was to determine the prevalence of Toxoplasma gondii in cattle from the Northern and Midwestern regions of Brazil. Serum samples were collected from 1789 animals and tested by indirect enzyme-linked immunosorbent assay (ELISA). The overall prevalence of T. gondii was 83.40% (1492/1789). The prevalence rates of T. gondii-seropositive animals observed in the states of Pará, Tocantins and, Mato Grosso were 87.45%, 87.79% and 73.06%, respectively. The detection of high prevalence rates of T. gondii in cattle deserves special attention because they are the main source of high biological value protein for humans. This finding indicates the need for further studies on the risk that these animals may pose to public health.
Tipo: Journal article
Palavras-chave: Cattle; ELISA; IFAT; Toxoplasma gondii.
Ano: 2015
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2015000100008

Nivel de conocimiento sobre toxoplasmosis en propietarios y su asociación con la seroprevalencia en Felis catus en La Habana
Provedor de dados: 101
Autores: Grandía,Raiden; Entrena,Ángel; Cruz,Jeddú; Ginorio,Dora; Domenech,Ingrid; Alfonso,Abdulahi; Perdomo,Loreley; Chi,Lourdes; Burón,Miriam.
En ocasiones, la prevalencia de Toxoplasma gondii está asociada con la baja percepción del riesgo de infección de este protozoo en la población humana, lo cual pudiera también influir negativamente sobre sus mascotas. Por ello, se trazó como objetivo determinar el nivel de conocimiento sobre toxoplasmosis en propietarios y su asociación con la seroprevalencia en Felis catus en La Habana. Se entrevistaron los 300 propietarios de los gatos muestreados para obtener respuestas a 10 preguntas relacionadas con: etiología, transmisión, sistema de órganos afectados, diagnóstico y control. Se realizó un análisis de frecuencia para determinar el nivel de conocimiento general y por temáticas (para el primero se asignaron 10 puntos a cada respuesta correcta, 100 en...
Tipo: Journal article
Palavras-chave: Desconocimiento; Propietarios; Toxoplasma gondii; Seroprevalencia; Felis catus.
Ano: 2013
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2013000200008

DETECTION OF INFECTION WITH Toxoplasma gondii IN MANATEES (Trichechus inunguis) OF THE PERUVIAN AMAZON
Provedor de dados: 37
Autores: DELGADO,PATRICK MATHEWS; PEREA,NOFRE SANCHEZ; MATHEWS DELGADO,JONH PAUL; GARCIA,CLAUDIA BIFFI; MALHEIROS,ANTONIO FRANCISCO; GARCIA DAVILA,CARMEN ROSA.
The Amazonian manatee (Trichechus inunguis) is an aquatic mammal that inhabits freshwater environments and is endemic to the Amazon Basin. The presence of Toxoplasma gondii antibodies was investigated in 19 manatees in one rescue unit in the northern region of Peru. Antibodies to T. gondii were detected in 12 (63.2 %) of 19 animals by using the modified agglutination test (titer, 1:25), and no association between sex and age of the animals and the presence of T. gondii antibodies was observed (p &lt; 0.05). The results suggest a contamination by T. gondii oocysts in the aquatic environment where these animals live.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazonian manatee; Peruvian Amazon; Serology; Toxoplasma gondii.
Ano: 2013
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2013000100017

Neosporosis y toxoplasmosis en la liebre europea (Lepus europaeus) en la provincia de La Pampa (Argentina)
Provedor de dados: 38
Autores: Baldone,Valeria Natalia; Fuchs,Lumila Ivana; Rojas,María del Carmen; Fort,Marcelo Cristian; Venturini,Cecilia; Giménez,Hugo Daniel.
El objetivo de este trabajo fue determinar la prevalencia a Neospora caninum (Nc) y Toxoplasma gondii (Tg) y conocer el periodo reproductivo de la liebre europea (Lepus europaeus) en la provincia de La Pampa (Argentina). Para ello se muestrearon 106 liebres procedentes de acopiadores de la provincia de La Pampa. Los sueros fueron analizados por un ensayo inmunoenzimático de competición para determinar anticuerpos a Nc y para Tg se realizó una prueba de hemaglutinación indirecta. Se encontraron 66 hembras, de las cuales 13 estaban preñadas, y 40 machos (p=0,01). Sobre un total de 44 sueros analizados para Nc, 5 (11,4%) dieron positivo. De los 106 sueros estudiados para Tg ninguno fue positivo.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animales silvestres; Ensayo inmunoenzimático; Hemaglutinación indirecta; Neospora caninum; Toxoplasma gondii.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572009000400009

High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil
Provedor de dados: 42
Autores: Belfort,RN; Nussenblatt,Veronique; Rizzo,Luiz; Muccioli,Cristina; Silveira,Claudio; Nussenblatt,Robert; Khan,Assis; Sibley,L. David; Belfort-Jr.,Rubens.
Toxoplasmosis is the most common cause of infectious uveitis in Brazil, with a higher frequency in the South of the country. We have collected samples from porcine tongue and diaphragm obtained in both large and small abattoirs and used molecular biological technique to determine the prevalence of infection and RFLP analysis to type the parasites. Seventeen out of 50 (34%) samples from the diaphragm and 33 out of 50 (66%) samples from the tongue demonstrated a positive PCR reaction for T. gondii and restriction analysis of four of the positive samples revealed that all had a type I genotype at SAG2. However, when other unlinked loci were analyzed, these strains had a type III genotype at markers BTUB, SAG3, and GRA6. One of the strains (8T) had a type II...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ocular toxoplasmosis; Toxoplasma gondii; Genotypes.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000100013

Acidification of the parasitophorous vacuole containing Toxoplasma gondii in the presence of hydroxyurea
Provedor de dados: 42
Autores: Carvalho,Cristiane S.; Melo,Edésio J.T.
Toxoplasma gondii multiplies within parasitophorous vacuole that is not recognized by the primary no oxidative defense of host cells, mainly represented by the fusion with acidic organelles. Recent studies have already shown that hydroxyurea arrested the intracellular parasites leading to its destruction. In the present work we investigated the cellular mechanism involved in the destruction of intracellular Toxoplasma gondii. Fluorescent vital stains were used in order to observe possible acidification of parasitophorous vacuole-containing Toxoplasma gondii in presence of hydroxyurea. Vero cells infected with tachyzoites were treated with hydroxyurea for 12, 24 or 48 hours. Fluorescence, indicative of acidification, was observed in the parasitophorous...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydroxyurea; Toxoplasma gondii; Parasitophorous vacuole.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000300008

Detection of Toxoplasma gondii by PCR and mouse bioassay in commercial cuts of pork from experimentally infected pigs
Provedor de dados: 45
Autores: Tsutsui,V.S.; Freire,R.L.; Garcia,J.L.; Gennari,S.M.; Vieira,D.P.; Marana,E.R.M.; Prudêncio,L.B.; Navarro,I.T.
The distribution of T. gondii in commercial cuts of pork (ham, tenderloin, spareribs and arm picnic) by PCR and bioassay from experimentally infected pigs, was evaluated. Eighteen mixed breed pigs were divided into two groups (G). The G1 animals (n=10) were infected with 4 x10(4) oocysts of the T. gondii VEG strain and the G2 animals (n=8) were used as control. Pigs of both groups were slaughtered at 59th day after infection, and meat samples were collected for bioassay and PCR. All animals from G1 were positive by at least one or both tests, and all control animals were negative. T. gondii was identified in pork by mouse bioassay and PCR in 27/40 (67.5%) and in 9/40 (22.5%) of the evaluated samples, respectively. There were no statistical differences in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pork meat; Mouse bioassay; PCR; Toxoplasma gondii.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000100006

Anticorpos em bovinos (Bos indicus e Bos taurus) e bubalinos (Bubalus bubalis) inoculados com oocistos de Toxoplasma gondii. Estudo comparativo
Provedor de dados: 45
Autores: Oliveira,F.C.R.; Costa,A.J.; Sabatini,G.A.
Três animais de cada espécie (Bos indicus, Bos taurus e Bubalus bubalis) foram inoculados, via oral, com 2×10(5) oocistos de Toxoplasma gondii. Seis outros animais, dois de cada espécie, foram mantidos como testemunhas. A resposta de anticorpos avaliada por meio da reação de imunofluorescência indireta iniciou-se a partir do quinto dia pós-inoculação (DPI) nos zebuínos e bubalinos, e no sétimo DPI nos taurinos. Os títulos sorológicos nos taurinos permaneceram elevados até o final do experimento (70º DPI), alcançando níveis máximos (1:16.384) entre o 42º e 49º DPI. Nos zebuínos e bubalinos o maior título de anticorpos anti-Toxoplasma foi de 1:256. A resposta de anticorpos mais ou menos acentuada não está necessariamente relacionada à sensibilidade ao T....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovino; Bubalino; Toxoplasma gondii; Anticorpo.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400007

Occurrence of antibodies to Neospora caninum and Toxoplasma gondii in dairy cattle from the northern region of the Paraná State, Brazil
Provedor de dados: 45
Autores: Ogawa,L.; Freire,R.L.; Vidotto,O.; Gondim,L.F.P.; Navarro,I.T.
Three-hundred and eighty-five serum samples were taken from dairy cows on 90 farms in 12 counties from the northern region of the Paraná State, Brazil. The samples were analyzed by IFAT for the detection of anti-Neospora caninum and anti-Toxoplasma gondii IgG antibodies. Forty-five (12%) samples were seropositive to N. caninum, while 102 (26%) samples were seropositive to T. gondii. Only four animals were seropositive to both coccidia. No significant difference was observed between the N. caninum serology and any of the variables studied, such as dairy cattle management, milk production, reproductive problems, feeding, and presence of dogs, cats and rodents. These data suggest that neosporosis is present among dairy cattle in the studied geographic region...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dairy cattle; Seroepidemiology; Neospora caninum; Toxoplasma gondii.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000300006

Toxoplasma gondii and Neospora caninum serological status of different canine populations from Uberlândia, Minas Gerais
Provedor de dados: 45
Autores: Mineo,T.W.P.; Silva,D.A.O.; Näslund,K.; Björkman,C.; Uggla,A.; Mineo,J.R.
Este trabalho teve como objetivo analisar a ocorrência de anticorpos contra Toxoplasma gondii e Neospora caninum em cães da cidade de Uberlândia, MG. As taxas de reatividade sorológica dos animais frente a esses parasitos foram comparadas em relação à sua origem e condições de vida. Um total de 369 amostras de soro canino foi obtido de três grupos de cães: (A) 213 pacientes do Hospital Veterinário da Universidade Federal de Uberlândia, (B) 62 pacientes de clínicas veterinárias particulares da cidade e (C) 94 cães errantes da cidade. As amostras foram analisadas para a presença de anticorpos contra os parasitos por meio de ELISA. Os resultados mostraram soropositividades de 30,3% para T. gondii, 9,2% para N. caninum e 5,7% para ambos os parasitos. O grupo...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dog; Serum profile; Toxoplasma gondii; Neospora caninum.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000300022

Vaccination of pigs with Toxoplasma gondii antigens incorporated in immunostimulating complexes (iscoms)
Provedor de dados: 45
Autores: Freire,R.L.; Navarro,I.T.; Bracarense,A.P.F.R.L.; Gennari,S.M.
Immunity to Toxoplasma gondii was studied in pigs, after vaccination with T. gondii antigens incorporated into immunostimulating complexes. Nine pigs (group 1 - G1) were inoculated subcutaneously with T. gondii iscoms (LIV-5 sample) and three doses were given at 21 and 13 day-intervals. The results were compared in other three groups of nine pigs each: animals in group 2 (G2) were immunized with the LIV-5 antigens without Quil A, animals in group 3 (G3) were inoculated with tachyzoites of RH T. gondii isolate, and animals in group 4 (G4) received no vaccination. Four animals were neither vaccinated nor challenged with T. gondii (group 5 - G5). Thirty days after vaccination, pigs were challenged orally with 5´10(4) oocysts at AS-28 T. gondii isolate....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Pig; Vaccine; Iscom.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000400002

Avaliação sorológica para Toxoplasma gondii pela imunofluorescência indireta e detecção do vírus da imunodeficiência felina pela nested PCR em felinos selvagens
Provedor de dados: 45
Autores: Rivetti Jr.,A.V.; Caxito,F.A.; Resende,M.; Lobato,Z.I.P.
Nineteen sera and blood samples from wild feline kept in captivity were tested for Toxoplasma gondii antibody and presence of feline immunodeficiency virus (FIV) DNA, respectively. Eighteen (94.7%) of the them were seropositive for toxoplasma. However, the only negative animal, a Leopardus pardalis, was the only FIV positve. These results suggest that the infection by FIV may have compromised its immune system and interfered with antibody production for toxoplasma.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Feline; Wild; Virus; Immunodeficiency; Toxoplasma gondii.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000500038

Toxoplasma gondii in milk of naturally infected dairy ewes on west mesoregion of Santa Catarina state, Brazil
Provedor de dados: 45
Autores: Ossani,R.A.; Borges,H.A.T.; Souza,A.P.; Sartor,A.A.; Miletti,L.C.; Federle,M.; Moura,A.B.
ABSTRACT This study aimed to detect Toxoplasma gondii in the milk of dairy sheep in the Western mesorregion of state of Santa Catarina by bioassay (22 milk samples from eight ewes seropositive; IFA ≥256) and PCR [for the detection of agent in the brains of mice inoculated on bioassay and directly from milk (108 samples from 42 seropositive ewes (IFA, ≥64) in different lactation periods)]. T. gondii DNA was detected in mice brains inoculated with milk from eight sheep (a sample of the 45th day of lactation and seven in the collection of 90th day) and directly from the milk in samples of the second collection (90 days) in five animals. Taking into account both assays, from a total of 42 ewes in lactation and seropositive for T. gondii, 30.95% (13/42) of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Milk sheep; PCR; Santa Catarina state.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501294

Genotyping of Toxoplasma gondii isolates from naturally infected Gallus domesticus in Santa Catarina state, Brazil
Provedor de dados: 45
Autores: Trevisani,N.; Barros,L.D.; Vieira-Neto,A.; Sartor,A.A.; Souza,A.P.; Garcia,J.L.; Moura,A.B.
ABSTRACT Toxoplasmosis is a widespread zoonosis that can infect warm-blooded animals including birds and humans, and chickens are considered to be indicators of environmental contamination. In Brazil, Toxoplasma gondii has a non-clonal population structure composed of three lineages (I, II, and III), presenting high recombination, and resulting in wide genetic diversity. This study aimed to genetically characterize T. gondii isolates from naturally infected chickens (Gallus domesticus) in Santa Catarina state, southern Brazil region. Sera from 133 free-range chickens were analyzed by an immunofluorescence assay (IFA) to detect IgG antibodies against T. gondii. Brain and heart from 30 positive animals, based on IFA (≥ 1:64), were used to isolate the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Gallus domesticus; Genotyping; Brazil.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000100139

Seroprevalence of toxoplasma gondii infection in goats in the five northwestern provinces of China
Provedor de dados: 45
Autores: Wang,Y.; Qin,X.; Chen,Y.; Xing,J.; Gao,J.; Qiu,D.
RESUMO O objetivo do presente estudo foi investigar a soroprevalência de infecção com Toxoplasma gondii (T. gondii) em cabras em cinco províncias do noroeste da China. Soroprevalência foi determinado usando o kit de teste de ensaio de imunoabsorção enzimática (ELISA). A soroprevalência geral foi 21.23% (197/928). Análise estatística revlou que diferenças significativas foram observadas em fêmeas (P= 0.048, OR= 0.567, 95% CI= 0.309 a 1.041) e nos grupos ≥ 2 (P= 0.002, OR= 0.330, 95% CI= 0.224 a 0.488). Nenhuma diferença estatisticamente significativa foi observada entre diferentes províncias. Nossos resultados indicam que a infecção com T. gondii, que pode ter implicações importantes sobre a saúde pública, teve diferenças significativas em sexo e idade, mas...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Toxoplasma gondii; Seroprevalence; Goat; Five northwestern provinces.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000100277

Prevalência da infecção por Toxoplasma gondii em galinhas criadas extensivamente em Rio Bonito, Rio de Janeiro
Provedor de dados: 45
Autores: Casartelli-Alves,L.; Ferreira,L.C.; Vicente,R.T.; Millar,P.R.; Oliveira,R.V.C.; Amendoeira,M.R.R.; Schubach,T.M.P.; Menezes,R.C.
The prevalence of T. gondii infection was investigated in free-range chickens for human consumption in the county of Rio Bonito, Rio de Janeiro, Brazil. Thus, a search for antibodies to T. gondii by indirect fluorescent antibody test was done in the serum of 220 chickens from twenty two farms. Titres equal or greater than 16 were considered positive. The prevalence of toxoplasmic infection found in chickens was 27.6% (61/220). There were positive cases in eighteen (77.2%) farms. The more frequent titre was 16 (12.5%) and the highest was 4096 (0.4%). The high prevalence of T. gondii in chickens and the high percentage of farms with positive cases indicate environmental contamination in the studied area showing the possibility of human and animal infection.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Galinha; Toxoplasma gondii; Reação de imunofluorescência indireta; Prevalência.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000500042

Risk factors for infection by Toxoplasma gondii in herds of goats in Ceará, Brazil
Provedor de dados: 45
Autores: Cavalcante,A.C.R.; Carneiro,M.; Gouveia,A.M.G.; Pinheiro,R.R.; Vitor,R.W.A.
In order to identify possible risk factors for T. gondii infection in goat herds in Ceará, Brazil, 2362 serum samples were tested by ELISA. The serological prevalence was 25.1%. The risk factors identified for Toxoplasma gondii infection in goat herds were age, number of cats, use of wooden feeding troughs and absence of feeding troughs. Goats older than 37 months had 2.01 (CI 95%; 1.55 - 2.61) higher risk of infection than younger animals. Greater risk of infection was observed in farms with more than 10 cats (OR = 1.73; CI 95%; 1.01 - 3.33). The use of wooden feeding troughs represented a high probability of infection (OR = 7.81; CI 95%; 1.66 - 36.67). The lack of feeding troughs also represented a high probability of infection (OR = 5.50; CI 95%; 1.24 -...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Goat; Prevalence; Toxoplasma gondii; Risk factor; Ceará.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000100006

Toxoplasmosis in emperor tamarin (Saguinus imperator): case report
Provedor de dados: 62
Autores: EPIPHANIO,Sabrina; CATÃO-DIAS,José Luiz; GUIMARÃES,Marcelo Alcindo de Barros Vaz.
A case of toxoplasmosis in an adult male emperor tamarin (Saguinus imperator) is reported. The primate was found dead and no clinical sign was noticed before death. Pathological findings included moderate to severe interstitial pneumonia, multifocal necrotizing hepatitis and multifocal to coalescing necrotizing lymphadenitis. Immunohistochemistry assays (strepto-avidin-biotin-peroxidase) performed on paraffin embedded tissues (lung, lymph nodes, liver, spleen, heart, intestine and adipose tissue) were strongly positive for Toxoplasma gondii.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Toxoplasma gondii; Primates; Saguinus imperator; Immunohistochemistry.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000200003

Ocorrência de anticorpos antitoxoplasma em gatos infectados naturalmente pelo vírus da imunodeficiência dos felinos
Provedor de dados: 62
Autores: LUCAS,Sílvia Regina Ricci; HAGIWARA,Mitika Kuribayashi; RECHE Jr.,Archivaldo; GERMANO,Pedro Manuel Leal.
Foram avaliadas, pela técnica de imunofluorescência indireta, a freqüência e a magnitude dos títulos de anticorpos antitoxoplasma (Toxoplasma gondii, Nicolle; Manceaux, 1909), em gatos infectados naturalmente pelo vírus da imunodeficiência dos felinos (VIF). Utilizaram-se 115 amostras de soro sangüíneo de gatos negativos ao vírus da leucemia felina que foram divididas em 3 grupos. Os 22 animais do grupo I eram positivos ao VIF. Os 58 animais que compuseram o grupo II eram doentes porém negativos na pesquisa de anticorpos anti-VIF e os 35 felinos do grupo III eram hígidos e negativos ao VIF. Os resultados obtidos permitiram concluir que a freqüência de anticorpos antitoxoplasma foi maior no grupo I do que nos grupos II e III. A análise estatística mostrou...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vírus da imunodeficiência felina; Toxoplasma gondii; Toxoplasmose felina.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961998000100008

Freqüência de anticorpos IgG anti-Toxoplasma gondii em soros de cães errantes da cidade de Salvador-Bahia, Brasil
Provedor de dados: 62
Autores: Barbosa,Marcus Vinícius Fróes; Guimarães,José Eugênio; Almeida,Maria Ângela Ornelas; Gondim,Luís Fernando Pita; Regis,Gustavo Barreto.
O Toxoplasma gondii é um coccídio intestinal intracelular obrigatório dos felídeos, de distribuição cosmopolita, descoberto em 1908 por Nicolle &amp;amp; Manceaux. O primeiro relato na espécie canina ocorreu em 1910, na Itália e, no Brasil em 1911. Objetivando-se avaliar a freqüência deste parasito, na população de cães errantes da cidade de Salvador-Ba, foram coletadas 225 amostras de sangue, de animais provenientes de 10 distritos sanitários. Os soros foram submetidos a reação de imunofluorescência indireta (RIFI) para detecção de anticorpos IgG anti-Toxoplasma gondii, utilizando-se a cepa AS28. Foram detectados 143 amostras positivas, representando uma freqüência de 63,55.00%. As freqüências nos distritos sanitários foram as seguintes: Itapagipe 33,33%;...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Toxoplasma gondii; Cães; Imunofluorescência indireta; Freqüência.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000600010

Toxoplasma gondii: I. Avaliação da virulência de oito amostras
Provedor de dados: 62
Autores: MITSUKA,Regina; BECKNER DA SILVA,Andrea Cristina; NAVARRO,Italmar Teodorico; BREGANÓ,José Wander; VIDOTTO,Odilon.
Oito amostras de T. gondii - LIV IV, LIV V e S 11 isoladas de suínos, RH e VPS de seres humanos, AS 28 de camundongo, HV III de cão e CN de gato - foram inoculadas em camundongos suíços albinos e em coelhos com o objetivo de avaliar a virulência e a patogenicidade. As oito amostras apresentaram-se altamente virulentas para camundongos, matando todos os animais que receberam inóculo, via intraperitoneal, de 10(4) taquizoítas, entre 6,0 e 7,8 dias, em média, após a inoculação. As amostras isoladas mais recentemente, LIV V e HV III (DL50 de 7 e 15 taquizoítas, respectivamente) foram as mais virulentas. A amostra RH foi a que apresentou a menor virulência, com DL50 de 3.160 taquizoítas. A amostra LIV V também se mostrou mais virulenta para coelhos, porém, como...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Patogenicidade; Animais de laboratório.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961998000100005

Evaluation of the performance of the modified direct agglutination test (MAT) for detection of Toxoplasma gondii antibodies in dogs
Provedor de dados: 62
Autores: Cañón Franco,William Alberto; Bergamaschi,Denise Pimentel; Richtzenhain,Leonardo José; Nogueira,Yeda; Camargo,Luís Marcelo Aranha; Souza,Silvio Luís Pereira de; Gennari,Solange Maria.
Toxoplasmosis is a zoonosis that has been the subject of study in Brazil and worldwide. The dogs are sentinels for the infection and can carry Toxoplasma gondii in the environment. Seroepidemiological surveys of these animals are an important tool in the surveillance and control of the disease and inform decision-making in health programs. In this study the performance of the Modified Agglutination Test (MAT) in the serodiagnosis of canine toxoplasmosis is evaluated and compared to the indirect immunofluorescent-antibody test (IFAT). A sample of 157 dog sera from the county of Monte Negro, Rondônia, with 76.40% positive reactions for Toxoplasma gondii (IFAT =16) was analyzed using the MAT (=25), presenting sensitivity of 85.00% (Confidence Interval 95.00%:...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Toxoplasma gondii; Dog; Agglutination tests; Sensitivity and specificity.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000600009

Toxoplasma gondii: II. Caracterização antigênica de taquizoítos de oito amostras
Provedor de dados: 62
Autores: MITSUKA,Regina; NAVARRO,Italmar Teodorico; BECKNER DA SILVA,Andrea Cláudia; BREGANÓ,José Wander; ALFIERI,Amauri; JANKEVICIUS,José Vitor; VIDOTTO,Odilon.
Oito amostras de T. gondii &lt;FONT FACE="Symbol"&gt;-&lt;/FONT&gt; LIV IV, LIV V e S 11 isoladas de suínos, RH e VPS de seres humanos, AS 28 de camundongo, HV III de cão e CN de gato &lt;FONT FACE="Symbol"&gt;-&lt;/FONT&gt; foram analisadas com o objetivo de verificar a existência de possíveis diferenças na resposta imune quando inoculadas em coelhos. Através da técnica de ELISA, não foram constatadas diferenças entre as oito amostras estudadas. Todas as amostras reagiram de forma semelhante com soros homólogos e heterólogos. A suspensão antigênica, constituída de extrato celular total, mostrou-se eficiente no ELISA teste indireto, já que os soros positivos reagiram fortemente e os soros negativos não apresentaram reação contra os antígenos testados. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Antígenos; ELISA teste indireto; Western blot.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961998000300003

Serological survey of antibodies to Toxoplasma gondii in food animals from São Paulo state, Brazil
Provedor de dados: 62
Autores: Meireles,Luciana Regina; Galisteo Jr.,Andrés Jimenez; Andrade Jr.,Heitor Franco de.
Toxoplasmosis is one of the most prevalent parasitic infections of man and livestock, and its transmission has usually been attributed to ingestion of undercooked or raw meat from infected livestock, with the infection rate in those animals being an important risk predictor of human disease, high in Brazil and São Paulo State. Looking for this public health problem, we tested serum samples from cattle, goat, sheep and chicken from the State of São Paulo, Brazil, for IgG antibodies to Toxoplasma gondii by enzyme-linked immunosorbent assay (ELISA). Antibodies to Toxoplasma gondii were found in 31.00% (62/200) of sheep, 17.00% (34/200) of goat and 11.00% (22/200) of cattle, without positive sample in chicken. Despite differences in feeding habits of each...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Food animals; Seroprevalence; ELISA.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000400005

Evaluation of experimental Toxoplasma gondii (Nicolle and Manceaux, 1909) infection in pigs by bioassay in mice and polymerase chain reaction
Provedor de dados: 62
Autores: Yai,Lucia Eiko Oishi; Vianna,Manoella Compostrini Barreto; Soares,Rodrigo Martins; Cortez,Adriana; Freire,Roberta Lemos; Richtznhain,Leonardo José; Gennari,Solange Maria.
The aim of the present experiment was to standardize a nested polymerase chain reaction (nPCR) for the detection of Toxoplasma gondii in tissues of experimentally infected pigs and to compare the performance of nPCR with the standard isolation technique, the bioassay in mice. Comparison between the two methods was done testing eight 4 month-old pigs orally inoculated with 5 x 10(4) oocysts of Toxoplasma gondii (AS-28 strain) and three non-infected pigs at the same age, kept as control. All animals were euthanatized 47 days after infection and samples of brain, heart, tongue and retina were collected from each animal for analysis by nPCR and bioassay in mice. By using the bioassay, Toxoplasma gondii was detected in 4 infected pigs, being two in the retina,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Pig; NestedPCR; Bioassay; Diagnosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000300010

The protective effects of Baypamun®HK in mice experimentally infected with Toxoplasma gondii
Provedor de dados: 62
Autores: Cunha,Eva Laurice Pereira; Silva,Rodrigo Costa da; Da Silva,Aristeu Vieira; Langoni,Hélio.
The present work aimed to verify the effects of Baypamun®HK on the survival of albinic mice, experimentally infected with Toxoplasma gondii RH strain, treated or not with sulfadiazine-pirimetamine, as well as on the formation and the number of brain cysts. Four groups of 20 mices were inoculated with 10(5) tachizoites, subcutaneously, and undergone to different treatments. Serum tests were carried out by indirect immunofluorescence and samples of brain, liver and lung were collected from animals who died for cytological and brain cysts examinations. After 60 days, all survivors were sacrificed. Isolated Baypamun®HK didn't present a protective action against the infection by T. gondii, but its association sulfadiazine-pirimetamine, a specific treatment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Baypamun®HK; Indirect immunofluorescence; Mice.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000300004

Congenital toxoplasmosis in a reference center of Paraná, Southern Brazil
Provedor de dados: 55
Autores: Capobiango,Jaqueline Dario; Breganó,Regina Mitsuka; Navarro,Italmar Teodorico; Rezende Neto,Claudio Pereira; Casella,Antônio Marcelo Barbante; Mori,Fabiana Maria Ruiz Lopes; Pagliari,Sthefany; Inoue,Inácio Teruo; Reiche,Edna Maria Vissoci.
This study describes the characteristics of 31 children with congenital toxoplasmosis children admitted to the University Hospital of Londrina, Southern Brazil, from 2000 to 2010. In total, 23 (85.2%) of the mothers received prenatal care but only four (13.0%) were treated for toxoplasmosis. Birth weight was &lt;2500 g in 37.9% of the infants. During the first month of life, physical examination was normal in 34.5%, and for those with clinical signs and symptoms, the main manifestations were hepatomegaly and/or splenomegaly (62.1%), jaundice (13.8%), and microcephaly (6.9%). During ophthalmic examination, 74.2% of the children exhibited injuries, 58.1% chorioretinitis, 32.3% strabismus, 19.4% microphthalmia, and 16.2% vitreitis. Anti-Toxoplasma gondii IgM...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Congenital toxoplasmosis; Chorioretinitis; Diagnosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000400364

Molecular diagnosis of symptomatic toxoplasmosis: a 9-year retrospective and prospective study in a referral laboratory in São Paulo, Brazil
Provedor de dados: 55
Autores: Camilo,Lilian Muniz; Pereira-Chioccola,Vera Lucia; Gava,Ricardo; Meira-Strejevitch,Cristina da Silva; Vidal,Jose Ernesto; Mattos,Cinara Cássia Brandão de; Frederico,Fábio Batista; De Mattos,Luiz Carlos; Spegiorin,Lígia Cosentino Junqueira Franco; Murata,Fernando Henrique Antunes; Ferreira,Marina Neves; Barbosa,Deusenia Machado Ulisses; Gonçalves,Fausto da Silva; Dias,Cristiane Moraes; Catelan,Marcia Wakai; Siqueira,Rubens Camargo; Previato,Mariana; Barbosa,Amanda Pires; Cavallini,Danilo.
ABSTRACT Symptomatic forms of toxoplasmosis are a serious public health problem and occur in around 10-20% of the infected people. Aiming to improve the molecular diagnosis of symptomatic toxoplasmosis in Brazilian patients, this study evaluated the performance of real time PCR testing two primer sets (B1 and REP-529) in detecting Toxoplasma gondii DNA. The methodology was assayed in 807 clinical samples with known clinical diagnosis, ELISA, and conventional PCR results in a 9-year period. All samples were from patients with clinical suspicion of several features of toxoplasmosis. According to the minimum detection limit curve (in CT), REP-529 had greater sensitivity to detect T. gondii DNA than B1. Both primer sets were retrospectively evaluated using 515...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Symptomatic toxoplasmosis; Diagnosis; Real-time polymerase chain reaction.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600638

Serum levels of neurotrophic factors in active toxoplasmic retinochoroiditis
Provedor de dados: 55
Autores: Cordeiro,Cynthia Azeredo; Vieira,Erica Leandro Marciano; Rocha,Natália Pessoa; Castro,Vinicius Monteiro; Oréfice,Juliana Lambert; Barichello,Tatiana; Costa,Rogerio Alves; Oréfice,Fernando; Young,Lucy; Teixeira,Antonio Lucio.
Abstract Toxoplasmic retinochoroiditis (TR) is the most common identifiable cause of posterior uveitis in Brazil. Response to treatment and clinical presentation may vary significantly. We assessed serum levels of brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), nerve growth factor (NGF), neurotrophin (NT)-3, and NT-4/5 in patients with active TR, before and after TR treatment.  Methods: Twenty patients with active lesion and 15 healthy controls were enrolled in the study. Serum concentration of neurotrophic factors was determined by enzyme-linked immunosorbent assay.  Results: BDNF levels were significantly higher in patients before treatment when compared with controls (p = 0.0015). There was no significant...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Retinochoroiditis; Toxoplasma gondii; Brain-derived neurotrophic factor.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000200176

Is chronic toxoplasmosis a risk factor for diabetes mellitus? A systematic review and meta-analysis of case-control studies
Provedor de dados: 55
Autores: Majidiani,Hamidreza; Dalvand,Sahar; Daryani,Ahmad; Galvan-Ramirez,Ma de la Luz; Foroutan-Rad,Masoud.
ABSTRACT  Introduction: The global protozoan parasite, Toxoplasma gondii, infects many warm-blooded animals and humans by employing different transmission routes. There have been some recent studies on the probable relevance of infectious agents and diabetes. Therefore, we conducted a systematic review and meta-analysis to identify the possible association between chronic toxoplasmosis and diabetes mellitus.  Methods: This study was conducted following the general methodology recommended for systematic reviews and meta-analysis. Nine English literature databases (Google scholar, PubMed, Scopus, Web of science, Science Direct, Ovid, ProQuest, IngentaConnect, and Wiley Online Library) were searched, up to January 2016. Random effects model was used to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Diabetes mellitus; Systematic review; Meta-analysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000600605

Postnatal acquired toxoplasmosis patients in an infectious diseases reference center
Provedor de dados: 55
Autores: Silva,Cassius Schnell Palhano; Neves,Elizabeth de Souza; Benchimol,Eliezer Israel; Moraes,Danielle Ribeiro de.
Infection caused by Toxoplasma gondii, toxoplasmosis, is one of the most frequent zoonoses in the world; it normally affects both genders equally. Humans are one of several possible intermediate hosts, and the disease is oligosymptomatic in most cases. Vertical transmission is an important cause of fetal malformation and sequels in newborns. Approximately 10% of postnatal cases present multiple manifestations, ranging from low fever and mild lymphadenopathy to severe encephalitis. In moderate cases, lesions such as retinochoroiditis may emerge during acute infection or even years later. We analyzed 313 cases of toxoplasmosis from 1992 to 2004, including 261 acute cases. Most patients were women (68.1%), and 39% of these were pregnant. Among acute infection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasmosis; Toxoplasma gondii; Survey; Clinical presentation; Retinochoroiditis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500016

Toxoplasma gondii infection in pregnancy
Provedor de dados: 55
Autores: Lopes,Fabiana Maria Ruiz; Gonçalves,Daniela Dib; Mitsuka-Breganó,Regina; Freire,Roberta Lemos; Navarro,Italmar Teodorico.
Toxoplasmosis is caused by an intracellular protozoan, Toxoplasma gondii, which has a wide geographical distribution. The main infection routes are ingestion of cysts from raw or badly-cooked meat, ingestion of oocysts from substrates contaminated with the feces of infected felines and congenital transmission by tachyzoites. The congenital form results in a severe systemic disease, because if the mother is infected for the first time during gestation, she can present a temporary parasitemia that will infect the fetus. Many of the clinical symptoms are seen in congenitally-infected children, from a mild disease to serious signs, such as mental retardation. Early diagnosis during the pregnancy is highly desirable, allowing prompt intervention in cases of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasmosis; Toxoplasma gondii; Congenital transmission; Pregnant women.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000500011

Effect of citrinin and in association with aflatoxin B1 on the infectivity and proliferation of Toxoplasma gondii in vitro
Provedor de dados: 55
Autores: Herzog-Soares,Joanna D'Arc A; Freire,Ronald Bastos.
Macrophages exposed to 10 mug/mL citrinin (CTR) or 0.01 mug CTR mixed with 0.04 mug aflatoxin B1 (AFB1) for a period of 2 h at 37ºC, were infected with 10(6) Toxoplasma gondii tachyzoites/muL. The parasites were treated with mycotoxins (2 h at 37ºC) before being added to the macrophage culture. The number of tachyzoites was quantified 2, 24, 48, 72 and 96 h after infection. During the first 2 hours, 59% infectivity was observed in the control. After exposure to CTR or the mixture of toxins (CTR-AFB1), macrophages were infected with 77.5% and 75% of the inoculated tachyzoites, respectively. Similarly, 72.3% of the cells were infected when cultured together with previously treated parasites. The treatment with CTR-AFB1 gave rise to 2.9 times more tachyzoites...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aflatoxins; Imunossuspression; Macrophages; Citrinin; Toxoplasma gondii.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000100007

Expression of extracellular matrix components and their receptors in the central nervous system during experimental Toxoplasma gondii and Trypanosoma cruzi infection
Provedor de dados: 56
Autores: Silva,A.A.; Roffê,E.; Lannes-Vieira,J.
Alterations in extracellular matrix (ECM) expression in the central nervous system (CNS) usually associated with inflammatory lesions have been described in several pathological situations including neuroblastoma and demyelinating diseases. The participation of fibronectin (FN) and its receptor, the VLA-4 molecule, in the migration of inflammatory cells into the CNS has been proposed. In Trypanosoma cruzi infection encephalitis occurs during the acute phase, whereas in Toxoplasma infection encephalitis is a chronic persisting process. In immunocompromised individuals such as AIDS patients, T. cruzi or T. gondii infection can lead to severe CNS damage. At the moment, there are no data available regarding the molecules involved in the entrance of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Trypanosoma cruzi; Central nervous system; Extracellular matrix.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500013

The use of confocal laser scanning microscopy to analyze the process of parasitic protozoon-host cell interaction
Provedor de dados: 56
Autores: de-Souza,W.; de-Carvalho,T.U.; de-Melo,E.T.; Soares,C.P.; Coimbra,E.S.; Rosestolato,C.T.; Ferreira,S.R.; Vieira,M.
In this communication we review the results obtained with the confocal laser scanning microscope to characterize the interaction of epimastigote and trypomastigote forms of Trypanosoma cruzi and tachyzoites of Toxoplasma gondii with host cells. Early events of the interaction process were studied by the simultaneous localization of sites of protein phosphorylation, revealed by immunocytochemistry, and sites of actin assembly, revealed by the use of labeled phaloidin. The results obtained show that proteins localized in the interaction sites are phosphorylated. The process of formation of the parasitophorous vacuole was monitored by labeling the host cell surface with fluorescent probes for lipids (PKH26), proteins (DTAF) and sialic acid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Confocal laser scanning microscopy; Host cell-parasite interaction; Trypanosoma cruzi; Toxoplasma gondii.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100015

Effect of hydroxyurea on the intracellular multiplication of Toxoplasma gondii, Leishmania amazonensis and Trypanosoma cruzi
Provedor de dados: 56
Autores: Melo,E.J.T.; Beiral,H.J.
Toxoplasma gondii, Leishmania amazonensis and Trypanosoma cruzi are obligate intracellular parasites that multiply until lysis of host cells. The present study was undertaken to evaluate the effect of hydroxyurea (an inhibitor of cell division at the G1/S phase) on the multiplication of L. amazonensis, T. gondii, and T. cruzi in infected host cells. Infected cells were treated with hydroxyurea (4 mM) for 48 h. Hydroxyurea arrested intracellular multiplication of all infective forms of the parasites tested. In treated cultures, the percent of infected host cells decreased (50-97%) and most intracellular parasites were eliminated. Ultrastructural observations showed no morphologic change in host cells while intracellular parasites presented drastic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Toxoplasma gondii; Trypanosoma cruzi; Leishmania amazonensis; Hydroxyurea.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100009

Induction of cell-mediated immunity during early stages of infection with intracellular protozoa
Provedor de dados: 56
Autores: Gazzinelli,R.T.; Talvani,A.; Camargo,M.M.; Santiago,H.C.; Oliveira,M.A.P.; Vieira,L.Q.; Martins,G.A.; Aliberti,J.C.S.; Silva,J.S.
Toxoplasma gondii and Trypanosoma cruzi are intracellular parasites which, as part of their life cycle, induce a potent cell-mediated immunity (CMI) maintained by Th1 lymphocytes and IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;. In both cases, induction of a strong CMI is thought to protect the host against rapid parasite multiplication and consequent pathology and lethality during the acute phase of infection. However, the parasitic infection is not eliminated by the immune system and the vertebrate host serves as a parasite reservoir. In contrast, Leishmania sp, which is a slow growing parasite, appears to evade induction of CMI during early stages of infection as a strategy for surviving in a hostile environment (i.e., inside the macrophages which are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Toxoplasma gondii; Trypanosoma cruzi; Macrophages; Cytokines; Nitric oxide.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100012

Prevalence of antibodies against Toxoplasma gondii and Neospora caninum in dogs
Provedor de dados: 58
Autores: Langoni,Helio; Fornazari,Felipe; Silva,Rodrigo Costa da; Monti,Elis Talita; Villa,Fausto Baptista.
Toxoplasmosis and neosporosis are parasitic diseases of global importance. The present study had the objective to determine the influence of age, sex and breed in the prevalence of antibodies against both diseases in dogs from Brotas city, São Paulo State, Brazil. Blood samples of 342 dogs were collected, and the age, sex and breed of each animal were recorded. The serological diagnosis for toxoplasmosis and neosporosis were performed using the immunofluorescent antibody test (IFAT). The Fischer's test was used to calculate the association probability of the variables, with a = 5%. For toxoplasmosis the prevalence of antibodies was 26.9% (CI 95% 22.4-31.8%), and for neosporosis 4.97% (CI 95% 3.1-7.8%). The statistical analysis revealed a higher risk of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Neospora caninum; IFAT.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400043

Clinical, parasitological and obstetric observations in pregnant bitches with experimental toxoplasmosis
Provedor de dados: 65
Autores: Bresciani,Katia Denise Saraiva; Toniollo,Gilson Hélio; Costa,Alvimar José da; Sabatini,Gustavo Adolfo; Moraes,Flávio Ruas de.
Eight pregnant mixed breed bitches, serologically negative for Toxoplasma gondii, were divided into three groups. Group I : bitches 01 and 02 (1.0 x 10(7) tachyzoites of Toxoplasma gondii, subcutaneous route); Group II: bitches 03, 04 and 05 (1.5 x 10(4) oocysts, oral route); Group III: bitches 06, 07 and 08 (as control). Clinical and obstetric examinations were conducted twice a day on each bitch, and weekly ultra-sonografic evaluations were performed until the end of pregnancy. From the 2nd day the bitches presented clinical alterations such as fever, nasal flowing, lachrymation, prostration, lymphadenopathy, premature parturition, abortion and fetal death. In Group I, Toxoplasma gondii was isolated from the bitches urine (day 10) and saliva (day 18)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasmosis; Toxoplasma gondii; Pregnant bitches; Pathology.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000600020

Molecular detection of protozoa of the Sarcocystidae family in sheep from the State of Rio Grande do Sul, Brazil
Provedor de dados: 65
Autores: Portella,Luiza Pires; Cadore,Gustavo Cauduro; Sangioni,Luis Antonio; Alves,Marta Elena Machado; Chemeris,Raiza; Brum,Larissa Picada; Vogel,Fernanda Silveira Flores.
ABSTRACT: Infections caused by protozoa belonging to the Sarcocystidae family have worldwide distribution and are common in ruminants, leading to considerable economic losses. This study evaluates Sarcocystis spp.,  Toxoplasma gondii and Neospora caninum infections in sheep from Southwest region of Rio Grande do Sul, Brazil. Myocardium samples of 80 sheep raised on extensive system were collected. Tissue cysts were detected by direct examination and presence of infective agents was confirmed by PCR. Macroscopic evaluation did not reveal changes, but direct microscopic examination showed cysts in 76.2% (61/80, 95% CI: 66.9 - 85.9) samples, and all cysts were morphologically similar to those caused by  Sarcocystis tenella or Sarcocystis arieticanis. PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sarcocystis spp.; Toxoplasma gondii; Neospora caninum; PCR; Diagnosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901613

Clínica e hematologia de Bos indicus, Bos taurus e Bubalus bubalis inoculados com oocistos de Toxoplasma gondii (Apicomplexa: Toxoplasmatinae)
Provedor de dados: 65
Autores: Oliveira,Francisco Carlos Rodrigues de; Costa,Alvimar José da; Sabatini,Gustavo Adolfo.
Três animais de cada espécie (Bos indicus, Bos taurus e Bubalus bubalis) foram inoculados, via oral, com 2,0 x 10(5) oocistos de Toxoplasma gondii. Seis outros animais, dois de cada espécie, foram mantidos como testemunhas. As alterações clínicas surgidas a partir do 3º dia após inoculação (DAI) foram: hipertermia, taquicardia, taquipnéia, anorexia, prostração, corrimento nasal e lacrimejamento. Estes sinais foram mais evidentes nos taurinos, espécie que apresentou, ainda, diarréia, fotofobia e conjuntivite. Foi possível isolar T. gondii da corrente sangüínea em todas as espécies. Nos taurinos, a partir do 5º DAI até o final do experimento, o parasito foi isolado de todas as amostras de sangue colhidas semanalmente, com exceção do 14º, 35º e 63º DAI. Os...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasmose; Toxoplasma gondii; Clínica; Hematologia; Bovino; Búfalo.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000400010

Detecção de anticorpos para Toxoplasma gondii em soro de suínos criados e abatidos em frigoríficos da região da grande Porto Alegre-RS, Brasil
Provedor de dados: 65
Autores: Fialho,Cristina Germani; Araujo,Flávio Antônio Pacheco de.
No presente trabalho, objetivou-se contribuir com dados sobre a freqüência de sororeagentes para Toxoplasma gondii em suínos criados e abatidos na Região da Grande Porto Alegre e fornecer subsídios sobre a importância da transmissão deste protozoário, por suínos. Foram coletadas amostras de 240 suínos em frigoríficos da região. A freqüência de anticorpos anti- Toxoplasma gondii, determinada através da técnica de hemaglutinação indireta, foi de 20 % de soros iguais ou superiores a diluição 1:64. Na técnica de imunofluorescência indireta, foram encontrados 33,75% de soro com diluição iguais a 1:16 ou superiores.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Suínos; Hemaglutinação indireta; Imunofluorescência indireta.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000500016

Occurrence of Toxoplasma gondii antibodies in lowland tapirs maintained ex situ in Brazil and Paraguay
Provedor de dados: 65
Autores: Gondim,Maria Fernanda Naegeli; Acosta,Igor da Cunha Lima; Rangel,Maria Cristina Valdetaro; Soares,Herbert Sousa; Gennari,Solange Maria; Dubey,Jitender Prakash; Cubas,Zalmir Silvino; Moraes,Wanderlei de; Chaparro,Francisco Javier Sosa; Barreto,Francisco Candido Cardoso; Rossi Junior,João Luiz.
ABSTRACT: Lowland Tapir ( Tapirus terrestris ) is the second largest South American land mammal. It is strictly herbivorous and its exposure to  Toxoplasma gondii should be indicative of environmental contamination by oocysts.In the present study antibodies to  T. gondii in 47 Brazilian tapirs maintained ex situ in 10 Brazilian and in one Paraguayan institution were sought in serum samples by the modified agglutination test (MAT ≥25). None of the animals presented clinical signs during the study. From 47 animals 35 (74.5%) were positive with titers of 25 in 8, 50 in 6, 100 in 12, 200 in 5, 400 in 1 and 800 in 3. One animal had samples collected on twice, and 19 were born in captivity. There was no association between occurrence of T. gondii antibodies and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ex situ; Lowland tapir; Tapirus terrestris; Toxoplasma gondii; Seroprevalence.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300502

The incidence and productive significance of ovine toxoplasmosis in Southern Brazil
Provedor de dados: 65
Autores: Oliveira,Fernando Caetano de; Oliveira,Plínio Aguiar de; Cunha Filho,Nilton Azevedo da; Aguiar,Cintia Lidiane Guidotti; Pappen,Felipe Geraldo; Ruas,Jeronimo Lopes; Farias,Nara Amélia da Rosa.
ABSTRACT: This research aimed to determine incidence of  Toxoplama gondii infection in Corriedale sheep in southern Brazil during pregnancy and the extent of associated losses. Blood samples were collected from 411 Corriedale sheep in two flocks at different locations in the state of Rio Grande do Sul (Brazil). Presence of  T. gondii was diagnosed by the Indirect Fluorescent Antibody Technique (IFAT). Seroprevalence of  T. gondii during pre-mating was 20.2%, with no significant difference between the two flocks studied. Infection by  T.  gondii was influenced by ewes'age (P≤0.05). The pre-mating seroprevalence did not influence either returning to estrus or pregnancy rates. Weaning rate was significantly higher in pre-positive dams compared to negative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Ewe; Pregnancy; Losses.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901618

Soroprevalência do toxoplasma gondii, em suínos, bovinos, ovinos e eqüinos, e sua correlação com humanos, felinos e caninos, oriundos de propriedades rurais do norte do Paraná-Brasil
Provedor de dados: 65
Autores: Garcia,João Luis; Navarro,Italmar Teodorico; Ogawa,Liza; Oliveira,Rosângela Claret de.
O Toxoplasma gondii, agente da toxoplasmose, encontra-se distribuído mundialmente. No presente estudo, determinou-se a prevalência do T. gondii nas espécies suína, bovina, ovina e eqüina oriundas de fazendas do município de Jaguapitã , através da imunofluorescência indireta para detecção de anticorpos da classe - IgG, considerando-se sororeagentes aqueles títulos maiores ou iguais a 1:64. Verificou-se uma prevalência de 24% em 267 amostras de suínos, 25,8% em 400 amostras de bovinos, 51,8% em 228 amostras de ovinos e 12,1% em 173 amostras de eqüinos. Com relação às faixas etárias, apenas os ovinos e suínos apresentaram diferenças estatísticas significantes, aumentando a soropositividade com as idades, porém, não se verificaram diferenças estatisticamente...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soroepidemiologia; Soroprevalência; Toxoplasma gondii; Suínos; Bovinos; Ovinos; Eqüinos.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781999000100017

Possibilidade da transmissão congênita de Toxoplasma gondii em ovinos através de seguimento sorológico no município de Rosário do Sul, RS, Brasil
Provedor de dados: 65
Autores: Silva,Karin Lucianne Monti de Vasconcellos; La Rue,Mário Luiz de.
No presente trabalho, descrevem-se os resultados da detecção de anticorpos anti-T. gondii (IgG e IgM) em amostras seqüenciais de soro de cordeiros recém-nascidos e de suas mães, sem aparentes sinais clínicos da infecção. Os anticorpos IgG foram quantificados pelos testes de imunofluorescência indireta (IFI) e hemaglutinação indireta (HAI). Os anticorpos IgM foram detectados pelo método de hemaglutinação indireta 2-mercaptoetanol (HAI/ 2-ME), utilizando "kits" comerciais. Foram considerados reagentes títulos maiores ou iguais a 16, na hemaglutinação, e 20 na imunofluorescência. Coletou-se soro de 247 ovelhas e cordeiros e, após três meses, somente dos cordeiros (n=120). Do total de animais, 175 eram de uma propriedade e 72, de outra propriedade, ambas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Ovelhas; Transmissão congênita; IgG; IgM; Rosário do Sul.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000300025

Toxoplasmose ocular em cães jovens inoculados com Toxoplasma gondii
Provedor de dados: 65
Autores: Abreu,Cláudia Bonini de; Navarro,Italmar Teodorico; Reis,Antônio Carlos Farias dos; Souza,Mírian Siliane Batista de; Machado,Roberto; Marana,Elizabete Regina Marangoni; Prudêncio,Leonardo Begale; Mattos,Marcos Rodrigues; Tsutsui,Vinícius Suehiro.
A toxoplasmose é uma doença zoonótica de distribuição mundial que pode infectar uma grande variedade de espécies animais e o homem. No presente trabalho, foram avaliados nove cães jovens inoculados experimentalmente com três cepas diferentes de T. gondii em que se observaram através de microscopia direta e indireta, alterações oftálmicas em sete deles, confirmadas através de retinografia e histopatologia. Com os resultados obtidos, observou-se que as alterações oftálmicas na toxoplasmose adquirida nos cães apareceram já ao término da terceira semana pós-inoculação, mostrando a precocidade das alterações. No exame direto do olho, sete cães apresentaram áreas de hiperrefletividade. Alterações como papiledema, exsudato peripapilar e diminuição da pigmentação...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasmose; Toxoplasma gondii; Toxoplasmose ocular; Cães.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000500011

Soroprevalência do Toxoplasma gondii em galinhas (Gallus gallus domesticus) de criações domésticas, oriundas de propriedades rurais do Norte do Paraná, Brasil
Provedor de dados: 65
Autores: Garcia,João Luis; Navarro,Italmar Teodorico; Ogawa,Liza; Marana,Elisabete Regina Marangoni.
O Toxoplasma gondii é um protozoário que pode infectar todos os animais homeotérmicos. No presente trabalho, verificou-se a ocorrência de anticorpos anti-T. gondii em galinhas de criações domésticas (fundo de quintal), oriundas de propriedades rurais localizadas no município de Jaguapitã, Estado do Paraná, Brasil. Estudos prévios demostraram que o parasita está amplamente disseminado nas diferentes espécies de animais domésticos, de companhia e no homem nessas mesmas propriedades. Os soros foram submetidos à reação de Imunofluorescência Indireta, sendo a positividade considerada para àqueles títulos maiores ou iguais a 16. Foram estudados 155 soros , sendo que 16 (10,3%) foram reagentes à toxoplasmose, e 139 (89,7%) não reagentes. Os títulos mais...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasmose em aves; Soroprevalência; Soroepidemiologia; Toxoplasma gondii.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000100020

Soroepidemiologia da toxoplasmose em gatos e cães de propriedades rurais do município de Jaguapitã, estado do Paraná, Brasil
Provedor de dados: 65
Autores: Garcia,João Luis; Navarro,Italmar Teodorico; Ogawa,Liza; Oliveira,Rosângela Claret de.
O presente estudo determinou a soroprevalência da toxoplasmose em gatos e cães localizados na zona rural do município de Jaguapitã, Estado do Paraná, através da reação de imunofluorescência indireta para detecção de anticorpos anti-Toxoplasma gondii da classe IgG, considerando-se reagentes títulos maiores ou iguais a 1:16. Observou-se uma prevalência de 73 % em 163 soros felinos e 84,1% em 189 soros caninos, sendo que nas duas espécies não houve diferença em relação ao sexo. Em relação aos felinos, o título mais freqüente foi 1: 4096 (28,6%) e o maior título encontrado foi de 1:65536 (0,8%), apresentando diferenças estatísticas com relação ás faixas etárias, e com aumento da soropositividade com a idade. Os títulos mais freqüentes nos caninos foram 1:64...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasmose; Soroprevalência; Cães; Gatos; Toxoplasma gondii.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781999000100018

Comportamento imunológico e antigênico de cinco amostras de Toxoplasma gondii inoculadas em gatos
Provedor de dados: 65
Autores: Navarro,Italmar Teodorico; Vidotto,Odilon; Silva,Andréa Carlos Bekner da; Mitsuka,Regina; Jankevicius,José Vitor; Shida,Paula Namie; Cortês,José de Angelis.
A biologia do Toxoplasma gondii demonstra que o gato é o hospedeiro completo, responsável pela disseminação do parasito. Assim, dois gatos domésticos foram imunizados com cada uma das amostras, VPS (humano), LIV-IV e LIV-V (suíno), CPL (caprino) e CN (felino) de T. gondii. Foram utilizados taquizoítas vivos em inóculos endovenosos de 2x10(6) (1° inóculo} e 4x10(7) (2° inóculo - 35 dias após), exceto a amostra VPS, onde 1 gato morreu no 10° dia com sinais clínicos agudos da doença, nas outras amostras, nenhum sinal clínico foi constatado durante os 6 meses de observação. O nível de anticorpos na imunização foi acompanhado através da reação de imunofluorescência indireta (IFI) com conjugado anti-IgG de felino. Os títulos de anticorpos obtidos no 20° dia...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Antígeno; Gatos.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781998000300017

Serum occurrence of anti-Toxoplasma gondii antibodies in dairy cows slaughtered in an abattoir for human consume
Provedor de dados: 65
Autores: Macedo,Madlaine Frigo Silveira Barbosa de; Macedo,Cesar Augusto Barbosa de; Barros,Luiz Daniel de; Martins,Guilherme Felipelli; Sandeski,Ligia Mara; Zulpo,Dauton Luiz; Cunha,Ivo Alexandre Leme da; Taroda,Alessandra; Cardim,Sérgio Tosi; Garcia,João Luis.
Toxoplasma gondii is a worldwide parasite recognized as one of the main zoonosis in human beings. The present study aimed to evaluate serology of T. gondii from dairy cows slaughtered in an abattoir for human consume. Serum samples from 120 dairy cows (60 pregnant and 60 non-pregnant) were collected, and indirect fluorescent antibody test (IFAT) was performed to detect anti-T. gondii antibodies by considering positive animals with titers ≥50. Serologic results from cows showed 29.1% (35/120), which 29 (48.3%) e 6 (10,0%) were from pregnant and non-pregnant cows, respectively. This revealed a risk 8.4 times-higher of positively in pregnant than non-pregnant cows (OR=8.4, 2.91&lt;OR&lt;25.6, P&lt;0.0001). There was a statistical difference in the anti-T....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Dairy cattle; Antibody occurrence.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000600019

Soroepidemiologia de Toxoplasma gondii em gatos domiciliados atendidos em clínicas particulares de Porto Alegre, RS, Brasil
Provedor de dados: 65
Autores: Pinto,Luciane Dubina; Araujo,Flávio Antônio Pacheco de; Stobb,Neusa Saltiél; Marques,Sandra Márcia Tietz.
Com o objetivo de contribuir com dados sobre freqüência de anticorpos para Toxoplasma em felinos domiciliados da cidade de Porto Alegre, Rio Grande do Sul, soros de 245 animais foram testados pelas técnicas sorológicas de Hemaglutinação Indireta (HAI) e Imunofluorescência Indireta (IFI). Informações sobre o animal, tais como raça, sexo, idade, acesso ou não à rua e tipo de alimentação, foram avaliadas por meio de questionário epidemiológico e análise estatística para correlacionar com os resultados obtidos na sorologia. Os resultados dos testes mostraram que os valores encontrados foram de 26,9% (HAI) e 37,9% (IFI), inferindo que esses gatos em algum momento de sua vida foram fonte de contaminação ambiental, sendo eliminadores potenciais de oocistos,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Gatos; Hemaglutinação Indireta; Imunofluorescência Indireta.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000800030

Dinâmica populacional de células mielóides durante o curso de inflamação crônica em camundongos C57BL/6 infectados com Toxoplasma gondii
Provedor de dados: 65
Autores: Fernandes,Bárbara Cruz Tavares de Macedo; Amendoeira,Maria Regina Reis; Freire,Ronald Bastos.
Fagócitos mononucleares (Mø) e polimorfo-nucleares neutrófilos (PMN) foram analisados durante o curso da infecção por Toxoplasma gondii em camundongos C57 BL/6, fêmeas, portadores de inflamações crônicas focais induzidas por material inerte. A dinâmica populacional de células mielóides dirigidas ao suporte inerte foi medida a partir do quarto dia após inoculação intraperitonial de 90-100 cistos de T. gondii, ao longo de vinte dias, e comparada com o percentual relativo de leucócitos sangüíneos. Observou-se uma diminuição do percentual de PMN e um aumento significativo de Mø no sangue dos animais infectados. A infecção assintomática por T. gondii em camundongos resultou em um maior esforço do sistema mielóide na produção de células destinadas a combater...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Células mielóides; Infecção crônica; Leucócitos.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000600024